Search Our Scientific Publications & Authors

J Vis Exp 2019 Apr 6(146). Epub 2019 Apr 6.

Baylor Global Health, Baylor College of Medicine; Department of Medicine, Baylor College of Medicine;

An uptick in recent pandemics (Ebola, Zika, MERS, influenza, etc.) underlines the need for a more 'nimble,' coordinated response that addresses a multitude of issues ranging from transportation, access, facilities, equipment, and communication to provider training. To address this need, we have developed an innovative, scalable, logistics-enhanced, mobile, laboratory facility for emergencies and epidemics in resource-constrained global settings. Read More

Int J Trichology 2019 Mar-Apr;11(2):82-85

Department of Microbiology, IGMC, Shimla, Himachal Pradesh, India.

White piedra is a superficial fungal infection of the hair shaft, caused by , a noncandidal yeast characterized by the presence of numerous, discrete, asymptomatic nodules attached to the infected hair shafts. White piedra is considered a disease of tropical regions and occasionally reported from temperate countries. Although such as is well known to cause cutaneous infections, it has been reported as a co-isolate for white piedra along with . Read More

Front Immunol 2019 29;10:706. Epub 2019 Mar 29.

Department of Molecular Virology and Microbiology, Baylor College of Medicine, Houston, TX, United States.

Recent studies of human sera showed that the majority of the respiratory syncytial virus (RSV) neutralizing antibodies are directed against pre-fusion conformation of the fusion (F) protein of RSV and revealed the importance of pre-fusion antigenic site Ø specific antibodies. However, detailed analysis of multiple antigenic site-specific competitive antibody responses to RSV F protein and their contribution to virus clearance in humans are lacking. We prospectively enrolled a cohort of RSV infected hematopoietic cell transplantation (HCT) adults ( = 40). Read More

J Infect Dis 2019 Apr 15. Epub 2019 Apr 15.

Department of Molecular Virology and Microbiology, Baylor College of Medicine, Houston, Texas.

Polymers (Basel) 2018 Dec 15;10(12). Epub 2018 Dec 15.

Institute for Optoelectronic Systems and Microtechnology (ISOM) and ETSI Industriales, Universidad Politécnica de Madrid, José Gutiérrez Abascal, 2, 28006 Madrid, Spain.

Polymers in highly confined geometries can display complex morphologies including ordered phases. A basic component of a theoretical analysis of their phase behavior in confined geometries is the knowledge of the number of possible single-chain conformations compatible with the geometrical restrictions and the established crystalline morphology. While the statistical properties of unrestricted self-avoiding random walks (SAWs) both on and off-lattice are very well known, the same is not true for SAWs in confined geometries. Read More

JAMA Pediatr 2019 Apr 1. Epub 2019 Apr 1.

Department of Emergency Medicine, Massachusetts General Hospital, Harvard Medical School, Boston.

Importance: Rhinovirus infection in early life, particularly with allergic sensitization, is associated with higher risks of developing recurrent wheeze and asthma. While emerging evidence links different rhinovirus species (eg, rhinovirus C) to a higher severity of infection and asthma exacerbation, to our knowledge, little is known about longitudinal associations of rhinovirus C infection during infancy with subsequent morbidities.

Objective: To examine the association of different viruses (respiratory syncytial virus [RSV], rhinovirus species) in bronchiolitis with risks of developing recurrent wheeze. Read More

Nat Ecol Evol 2019 04 18;3(4):561-569. Epub 2019 Mar 18.

Laboratory of Vector Control Research, Unit Transmission Reservoir and Pathogen Diversity, Institute Pasteur of Guadeloupe, Les Abymes, Guadeloupe, France.

Zika virus (ZIKV), discovered in the Zika Forest of Uganda in 1947, is a mosquito-borne flavivirus related to yellow fever, dengue and West Nile viruses. From its discovery until 2007, only sporadic ZIKV cases were reported, with mild clinical manifestations in patients. Therefore, little attention was given to this virus before epidemics in the South Pacific and the Americas that began in 2013. Read More

Pediatr Infect Dis J 2019 Apr;38(4):355-361

From the Department of Pediatrics.

Background: Molecular diagnostic methods enhance the sensitivity and broaden the spectrum of detectable respiratory viruses in febrile infants ≤90 days of life. We describe the occurrence of respiratory viruses in this population, as well as the rates of serious bacterial infection (SBI) and respiratory viral coinfection with regard to viral characteristics.

Methods: This was a prospective observational cohort study performed in the emergency department that included previously healthy febrile infants ≤90 days of life. Read More

BMC Pharmacol Toxicol 2019 02 22;20(1):14. Epub 2019 Feb 22.

National Institute of Respiratory Diseases "Ismael Cosío Villegas", 14080, Mexico City, Mexico.

AbstractFollowing publication of the original article [1], the author reported their given name have been erroneously tagged as their family names. Read More

Front Microbiol 2019 6;10:35. Epub 2019 Feb 6.

Institute of Evolutionary Ecology and Conservation Genomics, University of Ulm, Ulm, Germany.

High dispersal rates are known to homogenize host's population genetic structure in panmictic species and to disrupt host local adaptation to the environment. Long-distance dispersal might also spread micro-organisms across large geographical areas. However, so far, to which extent selection mechanisms that shape host's population genetics are mirrored in the population structure of the enteric microbiome remains unclear. Read More

Allergy 2019 Jan 17. Epub 2019 Jan 17.

Department of Emergency Medicine, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts.

J Allergy Clin Immunol 2019 Jan 14. Epub 2019 Jan 14.

Department of Emergency Medicine, Massachusetts General Hospital, Harvard Medical School, Boston, Mass.

Cad Saude Publica 2019 10;35(1):e00172417. Epub 2019 Jan 10.

Centro de Salud Familiar Piedra del Águila, Angol, Chile.

BMC Pharmacol Toxicol 2019 Jan 11;20(1). Epub 2019 Jan 11.

National Institute of Respiratory Diseases "Ismael Cosío Villegas", 14080, Mexico City, Mexico.

Background: Fibrosis is a response to chronic liver disease that results in excessive accumulation of extracellular matrix proteins and formation of scar tissue. Fibrosis represents a clinical challenge of worldwide significance. Several studies have demonstrated that many natural products and herbal medicines have activity against liver fibrosis, and extracts of milk thistle such as silymarin and silybin are the natural compounds most commonly prescribed for liver diseases. Read More

Pediatrics 2019 Feb 7;143(2). Epub 2019 Jan 7.

Departments of Molecular Virology and Microbiology and Pediatrics, Baylor College of Medicine, Houston, Texas

Endocrinol Diabetes Nutr 2019 Jan 6;66(1):41-48. Epub 2018 Jul 6.

Servicio de Endocrinología, Hospital Universitario Marqués de Valdecilla, Instituto de Investigación Marqués de Valdecilla (IDIVAL), Universidad de Cantabria, , Santander (Cantabria), España.

Introduction: The need for parathyroidectomy to treat asymptomatic patients with primary hyperparathyroidism is controversial. The aim of this study was to assess the impact of parathyroidectomy vs. surveillance on skeletal outcomes such as bone mineral density (BMD) and incident fractures. Read More

J Infect Dis 2018 Dec 25. Epub 2018 Dec 25.

Emergency Medicine, Massachusetts General Hospital, Harvard Medical School, Boston, MA.

The relation of nasopharyngeal microbiota to the clearance of respiratory syncytial virus (RSV) in infants hospitalized for bronchiolitis is not known. In a multicenter cohort, we found 106 of 557 (19%) infants hospitalized with RSV bronchiolitis had the same RSV subtype 3 weeks later (i.e. Read More

Clin Infect Dis 2018 Dec 11. Epub 2018 Dec 11.

Institute for Medical Immunology, Université libre de Bruxelles, Charleroi, Belgium.

Medwave 2018 Oct 16;17(6):e7288. Epub 2018 Oct 16.

Consulta Externa de Nutrición Clínica, Hospital Médica Sur, Tlalpan, México. Address: Puente de Piedra 150, Toriello Guerra, Tlalpan, Ciudad de México, México, CP: 14050. Email:

Introduction: Chronic diseases are on the rise and are associated with weight gain. Multidisciplinary strategies are required for its control.

Methods: The design was descriptive, observational and retrospective. Read More

Pancreatology 2019 Jan 23;19(1):122-126. Epub 2018 Nov 23.

Gastroclinica WGO Training Center, Tibás, San José, Costa Rica.

Background: Serous cystadenomas are benign lesions of the pancreas. Usually they are diagnosed incidentally on cross-sectional imaging studies. Endosonography is a valuable tool in the diagnosis and follow-up of these cystic lesions. Read More

Sci Rep 2018 Nov 26;8(1):17346. Epub 2018 Nov 26.

Department of Plant Science, Rothamsted Research, Harpenden, Hertfordshire, AL5 2JQ, UK.

Seeds exhibit wide variation in the fatty acid composition of their storage oil. However, the genetic basis of this variation is only partially understood. Here we have used a multi-parent advanced generation inter-cross (MAGIC) population to study the genetic control of fatty acid chain length in Arabidopsis thaliana seed oil. Read More

World Allergy Organ J 2018 12;11(1):26. Epub 2018 Nov 12.

1Health Economics Research Group, University of Cartagena, Campus Piedra de Bolívar, Cartagena, Colombia.

Background: Asthma is one of the most common chronic respiratory conditions worldwide. Asthma-related economic burden has been reported in Latin America, but knowledge about its economic impact to the Colombian health care system and the influence of disease severity is lacking. This study estimated direct medical costs and health care resource utilization (HCRU) in patients with asthma according to severity in Colombia. Read More

Pulm Pharmacol Ther 2018 12 3;53:116-120. Epub 2018 Nov 3.

Department of Experimental Medicine and Surgery, Chair of Respiratory Medicine, University of Rome 'Tor Vergata', Via Montpellier, 100133, Rome, Italy.

Background: Quitting smoking is key for patients with Chronic Obstructive Pulmonary Disease (COPD). Standard recommendations for quitting smoking are implemented for COPD as well. Varenicline Tartrate (VT) is the most effective drug to help quit smoking, but few studies have analysed its effectiveness. Read More

J Asthma 2018 Nov 5:1-9. Epub 2018 Nov 5.

d Unidad de Investigación , Hospital Médica Sur , Puente de Piedra 150, Colonia Toriello Guerra , Ciudad de México , CP. 14050 , México.

Objective: To identify and characterize asthma with blood eosinophilia in adults.

Methods: This cross-sectional study consisted of 164 asthma patients, aged 18 years or older. Multivariate analyses by logistic regression were performed to identify clinical characteristics and biomarkers associated with asthma with blood eosinophilia (defined as asthma and a peripheral blood eosinophil count ≥400 cells/mm). Read More

Vaccine 2018 Dec 30;36(52):8069-8078. Epub 2018 Oct 30.

Novavax, Inc., Gaithersburg, MD, USA. Electronic address:

Human respiratory syncytial virus (RSV) is the leading cause of severe lower respiratory tract infections in newborns, young children, elderly, and immune-compromised. The RSV fusion (F) glycoprotein is a major focus of vaccine development and the target of palivizumab (Synagis®) which is licensed as an immuno-prophylactic for use in newborn children at high risk of infection. However, clinical use of a narrowly targeted monoclonal antibodies leads to the generation of escape mutant strains that are fully resistant to neutralization by the antibody. Read More

Gac Med Mex 2018;154(4):509-519

Departamento de Cirugía de Cabeza y Cuello. Secretaría de Salud, Instituto Nacional de Cancerología, Ciudad de México, México.

Los pacientes afectados por el cáncer diferenciado de tiroides habitualmente presentan un curso clínico favorable, ya que la piedra angular del tratamiento es la cirugía; a pesar de esto, algunos pueden desarrollar un ominoso desenlace, debido a las características clinico-patológicas de esta enfermedad. El tratamiento óptimo aún es controvertido, en especial respecto a la extensión de la cirugía, indicaciones de radioyodo y la supresión de la hormona estimulante de la tiroides. La correcta evaluación de los riesgos, antes y después de la cirugía, facilita un selectivo enfoque del tratamiento; destacando la relevancia de revisar el impacto de la medicina nuclear en la correcta evaluación, tratamiento y seguimiento de los pacientes que padecen esta neoplasia. Read More

J Allergy Clin Immunol 2019 Apr 18;143(4):1371-1379.e7. Epub 2018 Sep 18.

Department of Emergency Medicine, Massachusetts General Hospital, Harvard Medical School, Boston, Mass.

Background: A better understanding of bronchiolitis heterogeneity might help clarify its relationship with the development of recurrent wheezing and asthma.

Objectives: We sought to identify severe bronchiolitis profiles using a clustering approach and to investigate for the first time their association with allergy/inflammatory biomarkers, nasopharyngeal microbiota, and development of recurrent wheezing by age 3 years.

Methods: We analyzed data from a prospective, 17-center US cohort study of 921 infants (age <1 year) hospitalized with bronchiolitis (2011-2014 winters) with posthospitalization follow-up. Read More

BMC Infect Dis 2018 Sep 18;18(1):470. Epub 2018 Sep 18.

Centro de Ciências da Saúde, Laboratório de Micologia Médica e Molecular, Departamento de Análises Clínicas e Toxicológicas, Universidade Federal do Rio Grande do Norte, Rua Gal. Gustavo Cordeiro de Faria S/N, Petrópolis, Natal, Rio Grande do Norte, Brazil.

Background: Trichosporon species may colonize the skin, respiratory tract and gastrointestinal tract of human beings. The yeast is recognized as etiological agent of white piedra, a superficial mycosis. Nevertheless, immunocompromised hosts may develop invasive Trichosporonosis. Read More

J Infect Dis 2019 Jan;219(4):514-516

Department of Molecular Virology and Microbiology and Department of Pediatrics, Baylor College of Medicine, Houston, Texas.

Clin Infect Dis 2018 Sep 12. Epub 2018 Sep 12.

Institute for Medical Immunology, Université libre de Bruxelles, Charleroi, Belgium.

Background: Epidemiological studies conducted in low and high-income countries showed that infants exposed to maternal HIV but not infected themselves by the virus have a high risk of severe infections. Immune alterations during fetal life have been proposed as a possible mechanism.

Methods: This prospective study was conducted to assess the relative risk of hospitalization for infection in HIV-exposed uninfected (HEU) infants as compared to HIV-unexposed (HU) infants born in a high-income country (HIC). Read More

Aging Male 2018 Aug 25:1-13. Epub 2018 Aug 25.

a Department of Biochemical Research , Institute of Sanitary Research Fundación Jiménez Díaz , Madrid , Spain.

Aim: The aim of this work is to compare the effects of osteoprotegerin (OPG) and testosterone on bone quality in a model of orchidectomised (ORX) rats.

Methods: Three-month-old ORX or SHAM operated groups (n = 15 each group) were used. The SHAM and ORX groups received saline. Read More

BMC Public Health 2018 Aug 3;18(1):964. Epub 2018 Aug 3.

David Geffen School of Medicine at UCLA, Los Angeles, CA, USA.

Background: Many older Hispanics/Latinos are physically inactive and suffer the harmful health consequences associated with prolonged periods of inactivity. Negative age attributions that equate getting older with "slowing down" reinforce this inactive behavior. We implemented a community-based exercise intervention among insufficiently active older Hispanics/Latinos with a randomized trial of an attribution-retraining program, ¡Caminemos! (Let's Walk!), and measured the effect of the program on walking behavior. Read More

Pediatr Infect Dis J 2019 Mar;38(3):e59-e62

From the Department of Emergency Medicine, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts.

In this analysis of 2 prospective multicenter, multi-year cohorts of children hospitalized for bronchiolitis in the United States and Finland, 306 rhinovirus infections were genotyped. Rhinovirus-A and -C species were predominant in the US study, while rhinovirus-C species was predominant in the Finland study. In both cohorts, there were no significant between-species differences in clinical characteristics, including acute severity measures. Read More

Lancet Infect Dis 2018 Oct 18;18(10):e295-e311. Epub 2018 Jun 18.

Laboratory of Translational Immunology, University Medical Centre Utrecht, Utrecht, Netherlands; Department of Paediatrics, Wilhelmina Children's Hospital, University Medical Centre Utrecht, Utrecht, Netherlands; Respiratory Syncytial Virus Network (ReSViNET) Foundation, Zeist, Netherlands. Electronic address:

The global burden of disease caused by respiratory syncytial virus (RSV) is increasingly recognised, not only in infants, but also in older adults (aged ≥65 years). Advances in knowledge of the structural biology of the RSV surface fusion glycoprotein have revolutionised RSV vaccine development by providing a new target for preventive interventions. The RSV vaccine landscape has rapidly expanded to include 19 vaccine candidates and monoclonal antibodies (mAbs) in clinical trials, reflecting the urgency of reducing this global health problem and hence the prioritisation of RSV vaccine development. Read More

Acta Trop 2018 Sep 11;185:380-384. Epub 2018 Jun 11.

Instituto Nacional de Antropología y Pensamiento Latinoamericano, Argentina; Universidad de Buenos Aires, Argentina.

This work aims to increase the information on the entero-parasitism in Holocene carnivores, by examining coprolites found in Patagonia. Molecular analysis was conducted following the Authenticity Criteria to Determine Ancient DNA sequences. The nucleotide sequences showed 99% of identity with the Control Region sequences of Lycalopex culpaeus (culpeo fox). Read More

J Eur Acad Dermatol Venereol 2019 Jan 28;33(1):e14-e16. Epub 2018 Jun 28.

Facultad de Ciencias, Universidad Nacional de San Antonio Abad del Cusco (UNSAAC), Cusco, Peru.

J Neurosurg Pediatr 2018 Sep 8;22(3):225-232. Epub 2018 Jun 8.

17Department of Neurosurgery, Baylor College of Medicine, Houston, Texas.

OBJECTIVE In children, the repair of skull defects arising from decompressive craniectomy presents a unique set of challenges. Single-center studies have identified different risk factors for the common complications of cranioplasty resorption and infection. The goal of the present study was to determine the risk factors for bone resorption and infection after pediatric cranioplasty. Read More

Dig Dis Sci 2018 10 4;63(10):2810-2812. Epub 2018 Jun 4.

Primary Systemic Vasculitides Clinic, Instituto Nacional de Enfermedades Respiratorias, Calzada de Tlalpan 4502, Col. Sección XVI, Tlalpan, C.P. 14080, Mexico City, Mexico.

Clin Exp Allergy 2018 Aug 4;48(8):1063-1067. Epub 2018 Jun 4.

Department of Emergency Medicine, Massachusetts General Hospital, Harvard Medical School, Boston, MA, USA.

Curr Allergy Asthma Rep 2018 05 9;18(5):33. Epub 2018 May 9.

Grupo de Alergología Clínica y Expermiental, IPS Universitaria, Universidad de Antioquia, Medellín, Colombia.

Purpose Of Review: Since omalizumab has been approved for urticaria, numerous randomized and real-life observational trials have been published. We reviewed the period January 2017-February 2018.

Recent Findings: Omalizumab is effective for the control of urticaria recalcitrant to antihistamines in different populations globally. Read More

J Infect Dis 2018 Jul;218(4):528-535

Department of Molecular Virology and Microbiology, Baylor College of Medicine, Houston, Texas.

Background: Other than influenza, little is known about the consequences of viral acute respiratory illness (ARI) on pregnant women and fetuses. Our objectives were to determine the frequency of ARI due to respiratory viruses and the associated clinical outcomes during pregnancy.

Methods: Pregnant women in their second or third trimester were enrolled if they reported having symptoms of ARI or were healthy within the preceding 2 weeks. Read More

J Nutr Biochem 2018 Oct 16;60:1-8. Epub 2018 Mar 16.

Translational Research Unit, Medica Sur Clinic & Foundation, Puente de Piedra 150, Toriello Guerra Tlalpan, C.P. 14050, Mexico City, Mexico. Electronic address:

Several microorganisms belonging to the intestinal microbiota act in an ecosystem responsible for maintaining the homeostasis and vital functions of human beings. From birth to old age the diversity of the intestinal microbiota may change due to environmental factors such as nutrition, immunity, diseases or the use of antibiotics leading to dysbiosis. Improvement in microbiota diversity can be achieved by modifying related risk factors through changes in lifestyle and a healthy diet. Read More

Aging Ment Health 2018 Apr 2:1-6. Epub 2018 Apr 2.

c Department of Medicine , David Geffen School of Medicine at UCLA and VA Greater Los Angeles Geriatric Research Education and Clinical Center , Los Angeles , CA , United States.

Objectives: We examined the prospective effects of an evidence-based exercise intervention on depressive symptoms in older Hispanics/Latinos and the potential synergistic effects (if any) of an attribution-retraining component to counter negative ascriptions to the aging process.

Method: We analyzed baseline, 1-, 12-, 24-month data collected from Hispanics/Latinos ≥ 60 years participating in an exercise intervention ("¡Caminemos!") across 27 senior centers (N = 572). All participants were given 4 weekly 1-hour group-based exercise classes targeting strength training, endurance, balance and flexibility. Read More

Mycoses 2018 Aug 13;61(8):543-548. Epub 2018 Jun 13.

Dermatology Resident, Fundación Universitaria Sanitas, Centro Dermatológico Federico Lleras Acosta, E.S.E., Bogotá, Colombia.

A cross-sectional descriptive study was conducted at a dermatology referral centre in Bogotá, to estimate the frequencies and aetiologies of mycoses in the population under 18 years of age attending the medical mycology laboratory over a 13-year period (2000-2012). A total of 1337 samples from 1221 patients were evaluated, involving direct examination and culture for 1279 samples, direct examination alone for 50 and culture alone for 8. During the study period, dermatophytosis was diagnosed via culture in 537 cases (40. Read More

PLoS One 2018 21;13(3):e0192731. Epub 2018 Mar 21.

Department of Evolutionary Ecology, Estación Biológica de Doñana (CSIC), Avda. Américo Vespucio 26, Seville, Spain.

Insular wildlife is more prone to extinction than their mainland relatives. Thus, a basic understanding of non-natural mortality sources is the first step in the development of conservation management plans. The Canary Islands are an important tourist destination due to their unique climate and rich scenery and biodiversity. Read More

Ecotoxicol Environ Saf 2018 Jul 20;156:216-222. Epub 2018 Mar 20.

Environmental and Computational Chemistry Group, Zaragocilla Campus. School of Pharmaceutical Sciences. University of Cartagena, Cartagena 130015, Colombia. Electronic address:

Atrazine and Glyphosate are herbicides massively used in agriculture for crop protection. Upon application, they are available to the biota in different ecosystems. The aim of this research was to evaluate the toxicity of Glyphosate and Atrazine based formulations (GBF and ABF, respectively). Read More

Eur Heart J Cardiovasc Imaging 2018 05;19(5):508-515

Department of Out patients Care, Echocardiography, Nuclear medicine National Instituto of Cardiology Ignacio Chávez, Juan Badiano No.1, Colonia Sección XVI, Tlalpan, Mexico City 14080, Mexico.

Aims: Ischaemic mitral regurgitation (IMR) is consequence of left ventricular (LV) remodelling after myocardial infarction. In some cases, the mitral valve enlarges to compensate for LV remodelling and tenting, improving its coaptation; a process termed 'plasticity'. We sought to identify clinical and echocardiographic factors associated with plasticity in patients with chronic inferior myocardial infarction (CII). Read More

J Infect Dis 2018 Mar;217(8):1247-1256

Department of Molecular Virology and Microbiology, Baylor College of Medicine, Houston, TX.

Background: Most respiratory syncytial virus (RSV) vaccine candidates include fusion (F) protein in different conformations. Antigenic site II found in the different F conformations is the target of palivizumab, the only US Food and Drug Administration approved monoclonal antibody (mAb). Serum palivizumab-like antibody (PLA) is a potential serologic correlate of immunity. Read More

Cutis 2017 Dec;100(6):448-450

Premier Dermatology, Abilene, Texas, USA.

The case of a 58-year-old man with a pruritic rash involving the pubic area that had been undiagnosed for 30 years is presented. At least 15 different primary care physicians and dermatologists evaluated the patient during this time period. Multiple treatments were unsuccessful and a definitive diagnosis was not rendered. Read More

Viral Immunol 2018 03 16;31(2):195-203. Epub 2018 Jan 16.

6 Department of Molecular Virology and Microbiology, Baylor College of Medicine , Houston, Texas.

Correlates of protection (CoPs) can play a significant role in vaccine development by assisting the selection of vaccine candidates for clinical trials, supporting clinical trial design and implementation, and simplifying tests of vaccine modifications. Because of this important role in vaccine development, it is essential that CoPs be defined by well-designed immunogenicity and efficacy studies, with attention paid to benefits and limitations. The respiratory syncytial virus (RSV) field is unique in that a great deal of information about the humoral response is available from basic research and clinical studies. Read More