2,113 results match your criteria Physiological genomics[Journal]


The Cardiac Endothelial Cell Transcriptome in Neonatal, Adult, and Remodeling Hearts.

Physiol Genomics 2019 Apr 12. Epub 2019 Apr 12.

University of Antwerp, Belgium.

Background - Cardiac microvascular endothelial cells (CMVECs) are the most numerous cells in the myocardium and orchestrate cardiogenesis during development, regulate adult cardiac function, and modulate pathophysiology of heart failure. It has been shown that the transcriptome of CMVECs differs from other endothelial cell types, but transcriptomic changes in cardiac endothelial cells during cardiac maturation and cardiac remodeling have not been studied earlier. Methods and Results - CMVECs were isolated from rat hearts based on CD31 expression and were immediately processed for RNA sequencing. Read More

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http://dx.doi.org/10.1152/physiolgenomics.00002.2019DOI Listing

Identification of suitable reference microRNA for qPCR analysis in pediatric inflammatory bowel disease.

Physiol Genomics 2019 Apr 12. Epub 2019 Apr 12.

Lurie Children's Hospital of Chicago, United States.

Pediatric inflammatory bowel disease (IBD) accounts for 10-15% of IBD and is associated with considerable morbidity for patients. Dysregulated microRNAs (miRNA), small non-coding RNA molecules that modulate gene expression, have been the target of research in IBD diagnosis, surveillance and therapy. Proper selection of reference genes, which are a prerequisite for accurate measurement of miRNA expression, are currently lacking. Read More

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http://dx.doi.org/10.1152/physiolgenomics.00126.2018DOI Listing
April 2019
1 Read

Genome-wide association study identifies the PLAG1-OXR1 region on BTA14 for carcass meat yield in cattle.

Physiol Genomics 2019 Mar 29. Epub 2019 Mar 29.

Laboratory of Molecular Biology and Bovine Breeding, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, China, China.

Carcass meat yield is an important carcass trait that contributes to the production efficiency and economic benefits in beef cattle. It is therefore critical to identify QTLs associated with carcass traits to enable selection. Our previous studies have identified several causal variants within the pleomorphic adenoma gene 1 ( PLAG1) and coiled-coil-helix-coiled-coil-helix domain containing 7 ( CHCHD7) genes on BTA14 for carcass traits in Chinese Simmental. Read More

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http://dx.doi.org/10.1152/physiolgenomics.00112.2018DOI Listing
March 2019
5 Reads

Profiling expression changes of genes associated with temperature and sex during high temperature induced masculinization in the Nile tilapia brain.

Physiol Genomics 2019 Mar 29. Epub 2019 Mar 29.

College of Animal Science and Veterinary Medicine, Shandong Agricultural University, China.

Fish sex-determining mechanisms can be classified as genotypic (GSD), temperature (TSD), or genotypic plus temperature effects (GSD+TE). Previous studies have shown that culturing water temperature during thermosensitive periods (TSP) could affect the expression of many genes in the gonad in some fish. However, few studies have focused on gene expression changes in the brain after temperature treatment during TSP in fish species. Read More

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http://dx.doi.org/10.1152/physiolgenomics.00117.2018DOI Listing
March 2019
1 Read

Salt-sensitive transcriptome of isolated kidney distal tubule cells.

Physiol Genomics 2019 Apr 15;51(4):125-135. Epub 2019 Mar 15.

Division of Nephrology and Hypertension, Department of Medicine, Oregon Health & Science University , Portland, Oregon.

In the distal kidney tubule, the steroid hormone aldosterone regulates sodium reabsorption via the epithelial sodium channel (ENaC). Most studies seeking to identify ENaC-regulating aldosterone-induced proteins have used transcriptional profiling of cultured cells. To identify salt-sensitive transcripts in an in vivo model, we used low-NaCl or high-NaCl diet to stimulate or suppress endogenous aldosterone, in combination with magnetic- and fluorescence-activated cell sorting to isolate distal tubule cells from mouse kidney for transcriptional profiling. Read More

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http://dx.doi.org/10.1152/physiolgenomics.00119.2018DOI Listing

Gene expression variability - the other dimension in transcriptome analysis.

Physiol Genomics 2019 Mar 15. Epub 2019 Mar 15.

ERIBA, University of Groningen, UMC Groningen, Netherlands.

Transcriptome sequencing is a powerful technique to study molecular changes that underlie the differences in physiological conditions and disease progression. A typical question that is posed in such studies is finding genes with significant changes between sample groups. In this respect expression variability is regarded as a nuisance factor that is primarily of technical origin and complicates the data analysis. Read More

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http://dx.doi.org/10.1152/physiolgenomics.00128.2018DOI Listing
March 2019
1 Read

Genetic and genomic evidence for an important role of the Na/H exchanger 3 in blood pressure regulation and angiotensin II-induced hypertension.

Physiol Genomics 2019 Apr 8;51(4):97-108. Epub 2019 Mar 8.

Laboratory of Receptor and Signal Transduction, Department of Pharmacology and Toxicology; Division of Nephrology, Internal Medicine; Cardiovascular and Renal Research Center; The University of Mississippi Medical Center , Jackson, Mississippi.

The sodium (Na)/hydrogen (H) exchanger 3 (NHE3) and sodium-potassium adenosine triphosphatase (Na/K-ATPase) are two of the most important Na transporters in the proximal tubules of the kidney. On the apical membrane side, NHE3 primarily mediates the entry of Na into and the exit of H from the proximal tubules, directly and indirectly being responsible for reabsorbing ~50% of filtered Na in the proximal tubules of the kidney. On the basolateral membrane side, Na/K-ATPase serves as a powerful engine driving Na out of, while pumping K into the proximal tubules against their concentration gradients. Read More

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http://dx.doi.org/10.1152/physiolgenomics.00122.2018DOI Listing
April 2019
1 Read

Chronic hypoxia changes gene expression profile of primary rat carotid body cells: consequences on the expression of NOS isoforms and ET-1 receptors.

Physiol Genomics 2019 Apr 1;51(4):109-124. Epub 2019 Mar 1.

Laboratorio de Neurobiología, Facultad de Ciencias Biológicas, Pontificia Universidad Católica de Chile , Santiago Chile.

Sustained chronic hypoxia (CH) produces morphological and functional changes in the carotid body (CB). Nitric oxide (NO) and endothelin-1 (ET-1) play a major role as modulators of the CB oxygen chemosensory process. To characterize the effects of CH related to normoxia (Nx) on gene expression, particularly on ET-1 and NO pathways, primary cultures of rat CB cells were exposed to 7 days of CH. Read More

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http://dx.doi.org/10.1152/physiolgenomics.00114.2018DOI Listing

Obesity "complements" preeclampsia.

Physiol Genomics 2019 Mar 4;51(3):73-76. Epub 2019 Feb 4.

Veterinary Clinical Sciences, School of Veterinary Medicine, Louisiana State University , Baton Rouge, Louisiana.

Preeclampsia (PE) is a devastating adverse outcome of pregnancy. Characterized by maternal hypertension, PE, when left untreated, can result in death of both mother and baby. The cause of PE remains unknown, and there is no way to predict which women will develop PE during pregnancy. Read More

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http://dx.doi.org/10.1152/physiolgenomics.00102.2018DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6459374PMC
March 2019
7 Reads

Activation of the cannabinoid receptor 2 increases renal perfusion.

Physiol Genomics 2019 Mar 1;51(3):90-96. Epub 2019 Feb 1.

Department of Pharmaceutical Sciences, College of Pharmacy, University of Tennessee Health Science Center , Memphis, Tennessee.

Acute kidney injury (AKI) is an increasing clinical problem that is associated with chronic kidney disease progression. Cannabinoid receptor 2 (CB2) activation has been shown to mitigate some of the deleterious tubular effects due to AKI, but its role on the renal vasculature has not been fully described. In this study, we investigated the effects of our novel CB2 receptor agonist, SMM-295, on renal vasculature by assessing cortical perfusion with laser Doppler flowmetry and changes in luminal diameter with isolated afferent arterioles. Read More

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http://dx.doi.org/10.1152/physiolgenomics.00001.2019DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6459373PMC

Role of gut metabolism of adrenal corticosteroids and hypertension: clues gut-cleansing antibiotics give us.

Physiol Genomics 2019 Mar 25;51(3):83-89. Epub 2019 Jan 25.

Division of Kidney Diseases and Hypertension, Rhode Island Hospital, Warren Alpert Medical School of Brown University , Providence, Rhode Island.

Intestinal bacteria can metabolize sterols, bile acids, steroid hormones, dietary proteins, fiber, foodstuffs, and short chain fatty acids. The metabolic products generated by some of these intestinal bacteria have been linked to a number of systemic diseases including obesity with Type 2 diabetes mellitus, some forms of inflammation, and more recently, systemic hypertension. In this review, we primarily focus on the potential role selected gut bacteria play in metabolizing the endogenous glucocorticoids corticosterone and cortisol. Read More

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http://dx.doi.org/10.1152/physiolgenomics.00115.2018DOI Listing
March 2019
9 Reads

Clock gene expression is altered in veterans with sleep apnea.

Physiol Genomics 2019 Mar 18;51(3):77-82. Epub 2019 Jan 18.

Department of Medicine, Division of Nephrology, University of Florida , Gainesville, Florida.

Clock gene dysregulation has been shown to underlie various sleep disorders and may lead to negative cardio-metabolic outcomes. However, the association between sleep apnea (SA) and core clock gene expression is unclear. We performed a cross-sectional analysis of 49 Veterans enrolled in a study of SA outcomes in veterans with chronic kidney disease, not selected for SA or sleep complaints. Read More

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http://dx.doi.org/10.1152/physiolgenomics.00091.2018DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6459375PMC
March 2019
2 Reads

microRNA-146a-5p association with the cardiometabolic disease risk factor TMAO.

Physiol Genomics 2019 Feb 11;51(2):59-71. Epub 2019 Jan 11.

US Department of Agriculture, Agricultural Research Service Western Human Nutrition Research Center, Obesity and Metabolism Unit, Davis, California.

Trimethylamine-N-oxide (TMAO), a microbial choline metabolism byproduct that is processed in the liver and excreted into circulation, is associated with increased atherosclerotic lesion formation and cardiovascular disease risk. Genetic regulators of TMAO levels are largely unknown. In the present study, we used 288 mice from a genetically heterogeneous mouse population [Diversity Outbred (DO)] to determine hepatic microRNA associations with TMAO in the context of an atherogenic diet. Read More

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http://dx.doi.org/10.1152/physiolgenomics.00079.2018DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6397334PMC
February 2019
6 Reads

Gene expression effects of lithium and valproic acid in a serotonergic cell line.

Physiol Genomics 2019 Feb 21;51(2):43-50. Epub 2018 Dec 21.

Carney Centre for Pharmacogenomics, Department of Pathology and Biomedical Science, University of Otago , Christchurch , New Zealand.

Valproic acid (VPA) and lithium are widely used in the treatment of bipolar disorder. However, the underlying mechanism of action of these drugs is not clearly understood. We used RNA-Seq analysis to examine the global profile of gene expression in a rat serotonergic cell line (RN46A) after exposure to these two mood stabilizer drugs. Read More

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http://dx.doi.org/10.1152/physiolgenomics.00069.2018DOI Listing
February 2019
1 Read

Differential circRNA expression profiles in latent human cytomegalovirus infection and validation using clinical samples.

Physiol Genomics 2019 Feb 21;51(2):51-58. Epub 2018 Dec 21.

Department of Clinical Laboratory, the Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University , Wenzhou, Zhejiang , China.

Human cytomegalovirus (HCMV) is an opportunistic prototypic beta-herpesvirus that can cause severe and even fatal diseases in immune-naive newborns and immunocompromised adults. Host-virus interactions occurring at the transcriptional and posttranscriptional levels are critical for establishing an HCMV latent or lytic infection, but the mechanisms remain poorly understood. Herein, we investigated the expression of circRNAs in human leukemia monocytes (THP-1 cells) latently infected with HCMV and explored the diagnostic value of circRNAs in children with HCMV infection. Read More

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http://dx.doi.org/10.1152/physiolgenomics.00096.2018DOI Listing
February 2019
1 Read

Identification of molecular signatures of cystic fibrosis disease status with plasma-based functional genomics.

Physiol Genomics 2019 Jan 12;51(1):27-41. Epub 2018 Dec 12.

Division of Endocrinology, Department of Pediatrics, Medical College of Wisconsin , Milwaukee, Wisconsin.

Although cystic fibrosis (CF) is attributed to dysfunction of a single gene, the relationships between the abnormal gene product and the development of inflammation and progression of lung disease are not fully understood, which limits our ability to predict an individual patient's clinical course and treatment response. To better understand CF progression, we characterized the molecular signatures of CF disease status with plasma-based functional genomics. Peripheral blood mononuclear cells (PBMCs) from healthy donors were cultured with plasma samples from CF patients ( n = 103) and unrelated, healthy controls ( n = 31). Read More

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https://www.physiology.org/doi/10.1152/physiolgenomics.00109
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http://dx.doi.org/10.1152/physiolgenomics.00109.2018DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6383551PMC
January 2019
20 Reads

Metabolic fitness in relation to genetic variation and leukocyte DNA methylation.

Physiol Genomics 2019 Jan 7;51(1):12-26. Epub 2018 Dec 7.

Department of Movement Sciences, KU Leuven, Leuven , Belgium.

Metabolic syndrome (MetS) is a highly prevalent condition causing increased risk of several life-threatening diseases. MetS has a pronounced hereditary basis but is also influenced by environmental factors, partly through epigenetic mechanisms. In this study, the five phenotypes underlying MetS were incorporated into a continuous score for metabolic fitness (MF), and associations with both genotypic variation and leukocyte DNA methylation were investigated. Read More

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http://dx.doi.org/10.1152/physiolgenomics.00077.2018DOI Listing
January 2019
1 Read

ATP2A2 rs3026468 does not associate with quadriceps contractile properties and acute muscle potentiation in humans.

Physiol Genomics 2019 Jan 7;51(1):10-11. Epub 2018 Dec 7.

School of Kinesiology, Faculty of Health Sciences, the University of Western Ontario , London , Canada.

The ATP2A2 gene encodes the SERCA protein required for active calcium reuptake to the sarcoplasmic reticulum in cardiac and slow-twitch skeletal muscle. The ATP2A2 rs3026468 variant has been associated with voluntary strength phenotypes in humans but requires further validation. Here we investigated a homogenous cohort of 80 young, healthy, active Caucasian males who were assessed for maximal isometric strength, voluntary activation, stimulated contractile properties, and muscle potentiation in the quadriceps. Read More

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http://dx.doi.org/10.1152/physiolgenomics.00085.2018DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6383552PMC
January 2019
3 Reads

Aging, melatonin biosynthesis, and circadian clockworks in the gastrointestinal system of the laboratory mouse.

Physiol Genomics 2019 Jan 16;51(1):1-9. Epub 2018 Nov 16.

Department of Biology, University of Kentucky , Lexington, Kentucky.

The gastrointestinal (GI) system is vital in its capacities for nutrient and water uptake, immune function, metabolism and detoxification, and stem-cell derived regeneration. Of significance to human health are a myriad of GI disorders associated with aging that integrate with the circadian clock. Here we present data from three groups of mice: young (3 mo old), middle aged (12 mo old), and old aged (24 mo old). Read More

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http://dx.doi.org/10.1152/physiolgenomics.00095.2018DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6383550PMC
January 2019
3 Reads

Impact of PYROXD1 deficiency on cellular respiration and correlations with genetic analyses of limb-girdle muscular dystrophy in Saudi Arabia and Sudan.

Physiol Genomics 2018 Nov 31;50(11):929-939. Epub 2018 Aug 31.

Division of Pediatric Neurology, Department of Pediatrics, University of Florida College of Medicine , Gainesville, Florida.

Next-generation sequencing is commonly used to screen for pathogenic mutations in families with Mendelian disorders, but due to the pace of discoveries, gaps have widened for some diseases between genetic and pathophysiological knowledge. We recruited and analyzed 16 families with limb-girdle muscular dystrophy (LGMD) of Arab descent from Saudi Arabia and Sudan who did not have confirmed genetic diagnoses. The analysis included both traditional and next-generation sequencing approaches. Read More

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http://dx.doi.org/10.1152/physiolgenomics.00036.2018DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6293114PMC
November 2018
13 Reads
1 Citation
2.374 Impact Factor

Sex-specific differences in primary neonatal murine lung fibroblasts exposed to hyperoxia in vitro: implications for bronchopulmonary dysplasia.

Physiol Genomics 2018 Nov 31;50(11):940-946. Epub 2018 Aug 31.

Department of Pediatrics, Section of Neonatology, Baylor College of Medicine , Houston, Texas.

Bronchopulmonary dysplasia (BPD) is a chronic lung disease of the neonate characterized by impaired alveolarization and vascular growth. BPD is more common in premature male infants, but the reasons underlying sexually dimorphic outcomes are not known. It is thought that alterations in fibroblast phenotype in response to environmental stress such as hyperoxia contribute to BPD. Read More

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https://www.physiology.org/doi/10.1152/physiolgenomics.00075
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http://dx.doi.org/10.1152/physiolgenomics.00075.2018DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6293119PMC
November 2018
12 Reads

Effect of high-fat diet feeding and associated transcriptome changes in the peak lactation mammary gland in C57BL/6 dams.

Physiol Genomics 2018 Dec 19;50(12):1059-1070. Epub 2018 Oct 19.

Department of Dairy Sciences, University of Wisconsin , Madison, Wisconsin.

Maternal consumption of a high-fat diet (HFD) during pregnancy has established adverse effects on the developing neonate. In this study, we aimed to investigate the effect of an HFD on the murine mammary gland during midlactation. Female C57BL/6J mice were placed on either a low-fat diet (LFD/10% fat) or HFD (60% fat) from 3 wk of age through peak lactation (lactation day 11/L11). Read More

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https://www.physiology.org/doi/10.1152/physiolgenomics.00052
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http://dx.doi.org/10.1152/physiolgenomics.00052.2018DOI Listing
December 2018
5 Reads

Comparative gene array analyses of severe elastic fiber defects in late embryonic and newborn mouse aorta.

Physiol Genomics 2018 Nov 12;50(11):988-1001. Epub 2018 Oct 12.

Department of Mechanical Engineering and Materials Science, Washington University , St. Louis, Missouri.

Elastic fibers provide reversible elasticity to the large arteries and are assembled during development when hemodynamic forces are increasing. Mutations in elastic fiber genes are associated with cardiovascular disease. Mice lacking expression of the elastic fiber genes elastin ( Eln), fibulin-4 ( Efemp2), or lysyl oxidase ( Lox) die at birth with severe cardiovascular malformations. Read More

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https://www.physiology.org/doi/10.1152/physiolgenomics.00080
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http://dx.doi.org/10.1152/physiolgenomics.00080.2018DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6293116PMC
November 2018
7 Reads
2.374 Impact Factor

Transcriptomic analysis of the development of skeletal muscle atrophy in cancer-cachexia in tumor-bearing mice.

Physiol Genomics 2018 Dec 5;50(12):1071-1082. Epub 2018 Oct 5.

Integrative Muscle Metabolism Laboratory, Exercise Science Research Center, Department of Health, Human Performance and Recreation, University of Arkansas , Fayetteville, Arkansas.

Cancer-cachexia (CC) is a wasting condition directly responsible for 20-40% of cancer-related deaths. The mechanisms controlling development of CC-induced muscle wasting are not fully elucidated. Most investigations focus on the postcachectic state and do not examine progression of the condition. Read More

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https://www.physiology.org/doi/10.1152/physiolgenomics.00061
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http://dx.doi.org/10.1152/physiolgenomics.00061.2018DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6337023PMC
December 2018
8 Reads

Identifying SNPs and candidate genes for three litter traits using single-step GWAS across six parities in Landrace and Large White pigs.

Physiol Genomics 2018 Dec 5;50(12):1026-1035. Epub 2018 Oct 5.

College of Animal Science and Technology, Sichuan Agricultural University , Chengdu, Sichuan , China.

Total number born (TNB), number born alive (NBA), and litter weight born alive (LWB) are critically important traits in pig production. The sow's parity is one of the major factors influencing litter traits. Because of monogenic or polygenic contributions and the presence of temporal gene effects in different sows' parities, it is difficult to clarify the biological and genetic background. Read More

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https://www.physiology.org/doi/10.1152/physiolgenomics.00071
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http://dx.doi.org/10.1152/physiolgenomics.00071.2018DOI Listing
December 2018
5 Reads

Proteome and transcriptome profiling of equine myofibrillar myopathy identifies diminished peroxiredoxin 6 and altered cysteine metabolic pathways.

Physiol Genomics 2018 Dec 5;50(12):1036-1050. Epub 2018 Oct 5.

Department of Population Health and Reproduction, University of California Davis , Davis, California.

Equine myofibrillar myopathy (MFM) causes exertional muscle pain and is characterized by myofibrillar disarray and ectopic desmin aggregates of unknown origin. To investigate the pathophysiology of MFM, we compared resting and 3 h postexercise transcriptomes of gluteal muscle and the resting skeletal muscle proteome of MFM and control Arabian horses with RNA sequencing and isobaric tags for relative and absolute quantitation analyses. Three hours after exercise, 191 genes were identified as differentially expressed (DE) in MFM vs. Read More

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https://www.physiology.org/doi/10.1152/physiolgenomics.00044
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http://dx.doi.org/10.1152/physiolgenomics.00044.2018DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6337024PMC
December 2018
3 Reads

Portfolio analysis on preeclampsia and pregnancy-associated hypertension research funded by the National Heart, Lung, and Blood Institute.

Physiol Genomics 2018 Nov 28;50(11):982-987. Epub 2018 Sep 28.

National Heart, Lung, and Blood Institute, National Institutes of Health, Division of Cardiovascular Sciences, Vascular biology and Hypertension branch, Bethesda, Maryland.

Chronic hypertension and preeclampsia are the most common complications of pregnancy. To clarify the contributions of the National Heart, Lung, and Blood Institute (NHLBI) to the field and identify potential research gaps, we performed portfolio analysis of awards related to preeclampsia and pregnancy-associated hypertension. A list of National Institutes of Health (NIH)-funded awards between fiscal years 2008-present was obtained through an NIH RePORTER search using the following terms: "preeclampsia" and "pregnancy-associated hypertension. Read More

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http://dx.doi.org/10.1152/physiolgenomics.00093.2018DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6293118PMC
November 2018
3 Reads

Identification of loci affecting sexually dimorphic patterns for height and recurrent laryngeal neuropathy risk in American Belgian Draft Horses.

Physiol Genomics 2018 Dec 28;50(12):1051-1058. Epub 2018 Sep 28.

Department of Clinical Sciences, Cornell University , Ithaca, New York.

Equine recurrent laryngeal neuropathy (RLN) is a bilateral mononeuropathy with an unknown etiology. In Thoroughbreds (TB), we previously demonstrated that the haplotype association for height (LCORL/NCAPG locus on ECA3, which affects body size) and RLN was coincident. In the present study, we performed a genome-wide association scan (GWAS) for RLN in 458 American Belgian Draft Horses, a breed fixed for the LCORL/NCAPG risk alelle. Read More

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https://www.physiology.org/doi/10.1152/physiolgenomics.00068
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http://dx.doi.org/10.1152/physiolgenomics.00068.2018DOI Listing
December 2018
4 Reads

Identification of differentially expressed genes in broiler offspring under maternal folate deficiency.

Physiol Genomics 2018 Dec 21;50(12):1015-1025. Epub 2018 Sep 21.

School of Agriculture and Forestry Sciences, Linyi University, Linyi, China.

Folate plays an important role in DNA and RNA synthesis by donating methyl groups. To investigate the effects of maternal folate deficiency (FD) on the abdominal adipose transcriptome and on the accumulation of lipid droplets in the liver tissue of chicken offspring, differentially expressed genes (DEGs) of FD were identified with digital gene expression tag profiling. Ultramicroscopy suggested that the size of lipid droplets in hepatocytes increased with FD, while the lipid droplets population number was largely not affected. Read More

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http://dx.doi.org/10.1152/physiolgenomics.00086.2018DOI Listing
December 2018
4 Reads

Epigenetics of metabolic syndrome.

Physiol Genomics 2018 Nov 21;50(11):947-955. Epub 2018 Sep 21.

Department of Genetics, Washington University School of Medicine , Saint Louis, Missouri.

The dramatic increase in global prevalence of metabolic disease is inexplicable when considering only environmental or only genetic factors, leading to the need to explore the possible roles of epigenetic factors. A great deal of progress has been made in this interdisciplinary field in recent years, with many studies investigating various aspects of the metabolic syndrome and its associated epigenetic changes. Rodent models of metabolic diseases have been particularly illuminating because of the ability to leverage tools such as genetic and environmental modifications. Read More

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http://dx.doi.org/10.1152/physiolgenomics.00072.2018DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6293117PMC
November 2018
14 Reads

Adverse early life environment induces anxiety-like behavior and increases expression of FKBP5 mRNA splice variants in mouse brain.

Physiol Genomics 2018 Nov 21;50(11):973-981. Epub 2018 Sep 21.

Division of Neonatology, Department of Pediatrics, Medical College of Wisconsin , Milwaukee, Wisconsin.

Adverse early life environment (AELE) predisposes adult offspring toward anxiety disorders. Anxiety disorders are associated with prenatal injuries in key regions of the brain including prefrontal cortex (PFC), hippocampus (HP), and hypothalamus (HT). Injuries in these brain regions result in an impaired hypothalamus-pituitary-adrenal axis (HPA axis) and stress response. Read More

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https://www.physiology.org/doi/10.1152/physiolgenomics.00054
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http://dx.doi.org/10.1152/physiolgenomics.00054.2018DOI Listing
November 2018
17 Reads

Comparative genomic analysis of the human and nematode Caenorhabditis elegans uncovers potential reproductive genes and disease associations in humans.

Physiol Genomics 2018 Nov 21;50(11):1002-1014. Epub 2018 Sep 21.

Department of Biomedical Science, College of Life Science, CHA University, Seongnam-si, Gyeonggi-do, Republic of Korea.

Reproduction is an important biological process. However, studies of human reproduction at the molecular level are limited due to the difficulty of performing in vivo studies. Hence, a mechanistic understanding of human reproduction remains still poor. Read More

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http://dx.doi.org/10.1152/physiolgenomics.00063.2018DOI Listing
November 2018
1 Read

Salt stress in the renal tubules is linked to TAL-specific expression of uromodulin and an upregulation of heat shock genes.

Physiol Genomics 2018 Nov 14;50(11):964-972. Epub 2018 Sep 14.

The British Heart Foundation Centre of Excellence, Institute of Cardiovascular and Medical Sciences, College of Medical, Veterinary, and Life Sciences, University of Glasgow , Glasgow , United Kingdom.

Previously, our comprehensive cardiovascular characterization study validated Uromodulin as a blood pressure gene. Uromodulin is a glycoprotein exclusively synthesized at the thick ascending limb of the loop of Henle and is encoded by the Umod gene. Umod mice have significantly lower blood pressure than Umod mice, are resistant to salt-induced changes in blood pressure, and show a leftward shift in pressure-natriuresis curves reflecting changes of sodium reabsorption. Read More

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http://dx.doi.org/10.1152/physiolgenomics.00057.2018DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6293113PMC
November 2018
9 Reads

Dysbiosis signatures of gut microbiota in coronary artery disease.

Physiol Genomics 2018 Oct 7;50(10):893-903. Epub 2018 Sep 7.

Department of Gastroenterology, Shanghai Tenth People's Hospital, Tongji University School of Medicine , Shanghai , China.

Gut microbiota dysbiosis has been considered to be an important risk factor that contributes to coronary artery disease (CAD), but limited evidence exists about the involvement of gut microbiota in the disease. Our study aimed to characterize the dysbiosis signatures of gut microbiota in coronary artery disease. The gut microbiota represented in stool samples were collected from 70 patients with coronary artery disease and 98 healthy controls. Read More

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http://dx.doi.org/10.1152/physiolgenomics.00070.2018DOI Listing
October 2018
21 Reads
2.370 Impact Factor

Fetal growth restriction shortens cardiac telomere length, but this is attenuated by exercise in early life.

Physiol Genomics 2018 Nov 7;50(11):956-963. Epub 2018 Sep 7.

School of Health and Life Sciences, Federation University Australia , Victoria , Australia.

Background And Aims: Fetal and postnatal growth restriction cause a predisposition to cardiovascular disease (CVD) in adulthood. Telomeres are repetitive DNA-protein structures that protect chromosome ends, and the loss of these repeats (a reduction in telomere length) is associated with CVD. As exercise preserves telomere length and cardiovascular health, the aim of this study was to determine the effects of growth restriction and exercise training on cardiac telomere length and telomeric genes. Read More

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http://dx.doi.org/10.1152/physiolgenomics.00042.2018DOI Listing
November 2018
2 Reads

Sex-specific differences in primary neonatal murine lung fibroblasts exposed to hyperoxia in vitro: implications for bronchopulmonary dysplasia.

Physiol Genomics 2018 Nov 31;50(11):940-946. Epub 2018 Aug 31.

Department of Pediatrics, Section of Neonatology, Baylor College of Medicine , Houston, Texas.

Bronchopulmonary dysplasia (BPD) is a chronic lung disease of the neonate characterized by impaired alveolarization and vascular growth. BPD is more common in premature male infants, but the reasons underlying sexually dimorphic outcomes are not known. It is thought that alterations in fibroblast phenotype in response to environmental stress such as hyperoxia contribute to BPD. Read More

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http://dx.doi.org/10.1152/physiolgenomics.00075.2018DOI Listing
November 2018
5 Reads

Molecular and genetic aspects of guanylyl cyclase natriuretic peptide receptor-A in regulation of blood pressure and renal function.

Authors:
Kailash N Pandey

Physiol Genomics 2018 Nov 31;50(11):913-928. Epub 2018 Aug 31.

Department of Physiology, Tulane University Health Sciences Center, School of Medicine , New Orleans, Louisiana.

Natriuretic peptides (NPs) exert diverse effects on several biological and physiological systems, such as kidney function, neural and endocrine signaling, energy metabolism, and cardiovascular function, playing pivotal roles in the regulation of blood pressure (BP) and cardiac and vascular homeostasis. NPs are collectively known as anti-hypertensive hormones and their main functions are directed toward eliciting natriuretic/diuretic, vasorelaxant, anti-proliferative, anti-inflammatory, and anti-hypertrophic effects, thereby, regulating the fluid volume, BP, and renal and cardiovascular conditions. Interactions of NPs with their cognate receptors display a central role in all aspects of cellular, biochemical, and molecular mechanisms that govern physiology and pathophysiology of BP and cardiovascular events. Read More

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https://www.physiology.org/doi/10.1152/physiolgenomics.00083
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http://dx.doi.org/10.1152/physiolgenomics.00083.2018DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6293115PMC
November 2018
4 Reads

Neuronal (pro)renin receptor regulates deoxycorticosterone-induced sodium intake.

Physiol Genomics 2018 Oct 24;50(10):904-912. Epub 2018 Aug 24.

Pharmacology and Department of Physiology and Cell Biology, University of Nevada, Reno, School of Medicine , Reno, Nevada.

Increased sodium appetite is a physiological response to sodium deficiency; however, it has also been implicated in disease conditions such as congestive heart failure, kidney failure, and salt-sensitive hypertension. The central nervous system is the major regulator of sodium appetite and intake behavior; however, the neural mechanisms underlying this behavior remain incompletely understood. Here, we investigated the involvement of the (pro)renin receptor (PRR), a component of the brain renin-angiotensin system, in the regulation of sodium intake in a neuron-specific PRR knockout (PRRKO) mouse model generated previously in our laboratory. Read More

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http://dx.doi.org/10.1152/physiolgenomics.00065.2018DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6230870PMC
October 2018
3 Reads

Effects of placental growth factor deficiency on behavior, neuroanatomy, and cerebrovasculature of mice.

Physiol Genomics 2018 Oct 17;50(10):862-875. Epub 2018 Aug 17.

Department of Biomedical and Molecular Sciences, Queen's University , Kingston, Ontario , Canada.

Preeclampsia, a hypertensive syndrome occurring in 3-5% of human pregnancies, has lifelong health consequences for fetuses. Cognitive ability throughout life is altered, and adult stroke risk is increased. One potential etiological factor for altered brain development is low concentrations of proangiogenic placental growth factor (PGF). Read More

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http://dx.doi.org/10.1152/physiolgenomics.00076.2018DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6230869PMC
October 2018
5 Reads

Disparate effects of antibiotics on hypertension.

Physiol Genomics 2018 Oct 10;50(10):837-845. Epub 2018 Aug 10.

Program in Physiological Genomics, Microbiome Consortium, Center for Hypertension and Personalized Medicine, Department of Physiology and Pharmacology, University of Toledo College of Medicine and Life Sciences , Toledo, Ohio.

Gut microbiota are associated with a variety of complex polygenic diseases. The usage of broad-spectrum antibiotics by patients affected by such diseases is an important environmental factor to consider, because antibiotics, which are widely prescribed to curb pathological bacterial infections, also indiscriminately eliminate gut commensal microbiota. However, the extent to which antibiotics reshape gut microbiota and per se contribute to these complex diseases is understudied. Read More

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http://dx.doi.org/10.1152/physiolgenomics.00073.2018DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6230872PMC
October 2018
46 Reads
1 Citation
2.374 Impact Factor

Global transcriptional differences in myokine and inflammatory genes in muscle of mature steer progeny are related to maternal lactation diet and muscle composition.

Physiol Genomics 2018 Oct 3;50(10):884-892. Epub 2018 Aug 3.

Department of Animal Sciences, Purdue University , West Lafayette, Indiana.

Steer progeny suckled by cows fed a dried distillers grains and solubles (DDGS) diet the first 3 mo of lactation were heavier during feedlot finishing and had significantly lower marbling and larger longissimus muscles than steers suckled by cows fed a control diet (CON). These differences were profound in that progeny were managed and fed identically from weaning until finishing, and findings suggest that the suckling period established the developmental program of muscle composition. Here transcriptomes of longissimus muscle were measured by next-generation sequencing to investigate whether there were any developmental clues to the differences in marbling scores and muscle content between steers suckled by DDGS ( n = 5) vs. Read More

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https://www.physiology.org/doi/10.1152/physiolgenomics.00060
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http://dx.doi.org/10.1152/physiolgenomics.00060.2018DOI Listing
October 2018
3 Reads

Exploring miRNA-mRNA regulatory network in cardiac pathology in Na/H exchanger isoform 1 transgenic mice.

Physiol Genomics 2018 Oct 20;50(10):846-861. Epub 2018 Jul 20.

Department of Pediatrics, University of California San Diego , La Jolla, California.

Numerous studies have demonstrated that Na/H exchanger isoform 1 (NHE1) is elevated in myocardial diseases and its effect is detrimental. To better understand the involvement of NHE1, we have previously studied cardiac-specific NHE1 transgenic mice and shown that these mice develop cardiac hypertrophy, interstitial fibrosis, and cardiac dysfunction. The purpose of current study was to identify microRNAs and their mRNA targets involved in NHE1-mediated cardiac injury. Read More

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http://dx.doi.org/10.1152/physiolgenomics.00048.2018DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6230871PMC
October 2018
2 Reads

High-throughput profiling of the circulating proteome suggests sexually dimorphic corticosteroid signaling following ischemic stroke.

Physiol Genomics 2018 Oct 20;50(10):876-883. Epub 2018 Jul 20.

Valtari Bio Incorporated, Morgantown, West Virginia.

Increasing evidence suggests that there are innate differences between sexes with respect to stroke pathophysiology; however, the molecular mechanisms underlying these differences remain unclear. In this investigation, we employed a shotgun approach to broadly profile sex-associated differences in the plasma proteomes of a small group of male ( n = 6) and female ( n = 4) ischemic stroke patients. Peripheral blood was sampled during the acute phase of care, and liquid chromatography electrospray ionization mass spectrometry was used to quantify plasma proteins. Read More

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http://dx.doi.org/10.1152/physiolgenomics.00058.2018DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6230873PMC
October 2018
2 Reads

R213G polymorphism in SOD3 protects against bleomycin-induced inflammation and attenuates induction of proinflammatory pathways.

Physiol Genomics 2018 Sep 13;50(9):807-816. Epub 2018 Jul 13.

Developmental Lung Biology and Cardiovascular Pulmonary Research Laboratories, Departments of Pediatrics and Medicine, University of Colorado, Anschutz Medical Campus, Aurora, Colorado.

Extracellular superoxide dismutase (EC-SOD), one of three mammalian SOD isoforms, is the sole extracellular enzymatic defense against superoxide. A known human single nucleotide polymorphism (SNP) in the matrix-binding domain of EC-SOD characterized by an arginine-to-glycine substitution at position 213 (R213G) redistributes EC-SOD from the matrix into extracellular fluids. We previously reported that knock-in mice harboring the human R213G SNP (R213G mice) exhibited enhanced resolution of inflammation with subsequent protection against fibrosis following bleomycin treatment compared with wild-type (WT) littermates. Read More

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http://dx.doi.org/10.1152/physiolgenomics.00053.2018DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6172610PMC
September 2018
26 Reads

Persistent pulmonary hypertension alters the epigenetic characteristics of endothelial nitric oxide synthase gene in pulmonary artery endothelial cells in a fetal lamb model.

Physiol Genomics 2018 Oct 13;50(10):828-836. Epub 2018 Jul 13.

Division of Neonatology, Department of Pediatrics, Medical College of Wisconsin , Milwaukee, Wisconsin.

Decreased expression of endothelial nitric oxide synthase (eNOS), a key mediator of perinatal transition, characterizes persistent pulmonary hypertension of the newborn (PPHN) in neonates and a fetal lamb model; the mechanisms are unclear. We investigated whether increased DNA CpG methylation at the eNOS promoter in estrogen response elements (EREs) and altered histone code together contribute to decreased eNOS expression in PPHN. We isolated pulmonary artery endothelial cells (PAEC) from fetal lambs with PPHN induced by prenatal ductus arteriosus constriction from 128 to 136 days gestation or gestation-matched twin controls. Read More

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http://dx.doi.org/10.1152/physiolgenomics.00047.2018DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6230868PMC
October 2018
10 Reads

Transcript profile distinguishes variability in human myogenic progenitor cell expansion capacity.

Physiol Genomics 2018 Oct 13;50(10):817-827. Epub 2018 Jul 13.

Division of Nutritional Sciences, Cornell University , Ithaca, New York.

Primary human muscle progenitor cells (hMPCs) are commonly used to understand skeletal muscle biology, including the regenerative process. Variability from unknown origin in hMPC expansion capacity occurs independently of disease, age, or sex of the donor. We sought to determine the transcript profile that distinguishes hMPC cultures with greater expansion capacity and to identify biological underpinnings of these transcriptome profile differences. Read More

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http://dx.doi.org/10.1152/physiolgenomics.00041.2018DOI Listing
October 2018
3 Reads

Influenza virus infection modulates the death receptor pathway during early stages of infection in human bronchial epithelial cells.

Physiol Genomics 2018 Sep 29;50(9):770-779. Epub 2018 Jun 29.

Allergy and Clinical Immunology Branch, Health Effects Laboratory Division, National Institute for Occupational Safety and Health, Centers for Disease Control and Prevention , Morgantown, West Virginia.

Host-viral interaction occurring throughout the infection process between the influenza A virus (IAV) and bronchial cells determines the success of infection. Our previous studies showed that the apoptotic pathway triggered by the host cells was repressed by IAV facilitating prolonged survival of infected cells. A detailed understanding on the role of IAV in altering the cell death pathway during early-stage infection of human bronchial epithelial cells (HBEpCs) is still unclear. Read More

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http://dx.doi.org/10.1152/physiolgenomics.00051.2018DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6388629PMC
September 2018
5 Reads

A mini review: Proteomics approaches to understand disused vs. exercised human skeletal muscle.

Physiol Genomics 2018 Sep 29;50(9):746-757. Epub 2018 Jun 29.

Division of Cardiology, Department of Medicine, University of California San Diego , La Jolla, California.

Immobilization, bed rest, or denervation leads to muscle disuse and subsequent skeletal muscle atrophy. Muscle atrophy can also occur as a component of various chronic diseases such as cancer, AIDS, sepsis, diabetes, and chronic heart failure or as a direct result of genetic muscle disorders. In addition to this atrophic loss of muscle mass, metabolic deregulation of muscle also occurs. Read More

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https://www.physiology.org/doi/10.1152/physiolgenomics.00043
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http://dx.doi.org/10.1152/physiolgenomics.00043.2018DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6172614PMC
September 2018
4 Reads

Genetics of guanylyl cyclase pathways in the cochlea and their influence on hearing.

Physiol Genomics 2018 Sep 29;50(9):780-806. Epub 2018 Jun 29.

Department of Biomedical Sciences, University of Minnesota Medical School , Duluth, Minnesota.

Although hearing loss is the most common sensory deficit in Western societies, there are no successful pharmacological treatments for this disorder. Recent experiments have demonstrated that manipulation of intracellular cyclic guanosine monophosphate (cGMP) concentrations can have both beneficial and harmful effects on hearing. In this review, we will examine the role of cGMP as a key second messenger involved in many aspects of cochlear function and discuss the known functions of downstream effectors of cGMP in sound processing. Read More

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http://dx.doi.org/10.1152/physiolgenomics.00056.2018DOI Listing
September 2018
5 Reads

Identification of nine novel loci related to hematological traits in a Japanese population.

Physiol Genomics 2018 Sep 29;50(9):758-769. Epub 2018 Jun 29.

Department of Human Functional Genomics, Advanced Science Research Promotion Center, Mie University, Tsu, Mie , Japan.

Recent genome-wide association studies have identified various genetic variants associated with hematological traits. Although it is possible that quantitative data of hematological traits are varied among the years examined, conventional genome-wide association studies have been conducted in a cross-sectional manner that measures traits at a single point in time. To address this issue, we have traced blood profiles in 4,884 Japanese individuals who underwent annual health check-ups for several years. Read More

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http://dx.doi.org/10.1152/physiolgenomics.00088.2017DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6172615PMC
September 2018
1 Read