Search Our Scientific Publications & Authors

Viruses 2019 Feb 13;11(2). Epub 2019 Feb 13.

School of Public Health (Shenzhen), Sun Yat-sen University, Guangdong 518107, China.

Antigen-specific T lymphocytes play a critical role in controlling viral infections. However, we report here that preexisting virus-specific T cell responses also contribute to promoting adenovirus (Ad) infection. Previously, we found that CD14+ monocytes from Ad-seropositive individuals exhibited an increased susceptibility to Ad infection, when compared with that of Ad-seronegative individuals. Read More

PLoS One 2019 19;14(2):e0211079. Epub 2019 Feb 19.

Central Virology Laboratory, Ministry of Health, Chaim Sheba Medical Center, Ramat-Gan, Israel.

Respiratory viral infections constitute the majority of samples tested in the clinical virology laboratory during the winter season, and are mainly diagnosed using molecular assays, namely real-time PCR (qPCR). Therefore, a high-quality extraction process is critical for successful, reliable and sensitive qPCR results. Here we aimed to evaluate the performance of the newly launched eMAG compared to the fully automated MagNA PURE 96 (Roche, Germany) and to the semi-automated easyMAG (bioMerieux, France) extraction platforms. Read More

Clin Lab 2019 Jan;65(1)

Background: Human bocavirus (HBoV) is known to cause lower respiratory tract infections (LRTI) in children and may result in substantial morbidity and mortality. The aim of this study was to determine HBoV prevalence among hospitalized infants and small children with acute LRTI in Zagreb, Croatia, as well as to evaluate HBoV DNA quantity in samples in relation to the patients' age and co-infection with other respiratory viruses.

Methods: During winter season 2016/2017, a total of 295 children younger than three years of age who were admitted to hospitals with LRTI were tested for the presence of HBoV, respiratory syncytial virus (RSV), adenovirus (ADV), parainfluenza virus (PIV) types 1 to 3, and human metapneumovirus (HMPV). Read More

BMJ 2019 Feb 13;364:l231. Epub 2019 Feb 13.

Department of Pediatrics, Østfold Hospital Trust, Grålum, Norway.

Objective: To determine whether infection with human enterovirus or adenovirus, both common intestinal viruses, predicts development of coeliac disease.

Design: Case-control study nested within Norwegian birth cohort recruited between 2001 and 2007 and followed to September 2016.

Setting: Norwegian population. Read More

Diagn Microbiol Infect Dis 2019 Jan 17. Epub 2019 Jan 17.

Department of Medicine and Surgery, University of Parma, Viale A. Gramsci 14, 43126 Parma, Italy.

Acute respiratory tract infections (ARTIs) are among the leading causes of morbidity and mortality in children. The viral etiology of ARTIs was investigated over 3 years (October 2012-September 2015) in 2575 children in Parma, Italy, using indirect immunofluorescent staining of respiratory samples for viral antigens, cell culture, and molecular assays. Respiratory viruses were detected in 1299 cases (50. Read More

Viruses 2019 Feb 8;11(2). Epub 2019 Feb 8.

Department of Microbiology and Immunology, Western University, London, ON N6A 3K7, Canada.

Viruses are obligate intracellular parasites that alter many cellular processes to create an environment optimal for viral replication. Reprogramming of cellular metabolism is an important, yet underappreciated feature of many viral infections, as this ensures that the energy and substrates required for viral replication are available in abundance. Human adenovirus (HAdV), which is the focus of this review, is a small DNA tumor virus that reprograms cellular metabolism in a variety of ways. Read More

Biomedicines 2019 Feb 2;7(1). Epub 2019 Feb 2.

Department of Medicine, State University of New York, Upstate Medical University, Syracuse, NY 13210, USA.

We would like to thank Dr. Atkinson for his comments on our review article on the manifestations of adenoviral infections in humans. [. Read More

Sci Rep 2019 Jan 30;9(1):929. Epub 2019 Jan 30.

Department of Laboratory Medicine, Royal Infirmary of Edinburgh, Edinburgh, UK.

Numerous viruses can cause upper respiratory tract infections. They often precede serious lower respiratory tract infections. Each virus has a seasonal pattern, with peaks in activity in different seasons. Read More

Bone Marrow Transplant 2019 Jan 29. Epub 2019 Jan 29.

Division of Hematology/Oncology, The University of North Carolina at Chapel Hill, Chapel Hill, NC, USA.

Limited data exist on characteristics of central nervous system viruses (CNS-V) in allogeneic hematopoietic stem cell transplant (HCT) recipients. Between 2007 and 2015, the Center for International Blood and Marrow Transplant Research (CIBMTR) received information on 27,532 patients undergoing HCT. Of these, centers reported 165 HCT recipients with CNS-V detected in cerebrospinal fluid within 6 months after HCT. Read More

Eur J Clin Microbiol Infect Dis 2019 Jan 29. Epub 2019 Jan 29.

Division of Pulmonary and Critical Care Medicine, Department of Medicine, Samsung Medical Center, Sungkyunkwan University School of Medicine, Ilwon-ro 81, Gangnam-gu, Seoul, Republic of Korea.

Human adenovirus (HAdV) is a common pathogen causing respiratory infections with outbreaks reported in the military and community. However, little information is available on the shedding kinetics. We performed a prospective study of immunocompetent adults confirmed with HAdV respiratory infection by multiplex real-time PCR during an outbreak of HAdV-55. Read More

J Pediatr Hematol Oncol 2019 Jan 25. Epub 2019 Jan 25.

Pediatric Infection.

Background: Acute viral respiratory infections are common causes of febrile episodes in children. There are still limited data about distribution of acute viral respiratory infections in children with cancer.

Objective: The first aim of this study was to evaluate the viral etiology and seasonality of acute viral respiratory infection in pediatric patients with cancer in a 3-year study. Read More

Antiviral Res 2019 Mar 21;163:50-58. Epub 2019 Jan 21.

University College London, London, UK.

Background: Double-stranded (ds) DNA virus infections often occur concomitantly in immunocompromised patients. We performed a systematic search of published in vitro activity for nine approved and investigational antivirals to understand the spectrum of in vitro activity against dsDNA viruses.

Methods: A literature search was performed (PubMed and the WoS Core Collection) using keywords related to: 1) targeted approved/developmental antivirals (acyclovir, artesunate, brincidofovir, cidofovir, cyclopropavir (filociclovir), foscarnet, ganciclovir, letermovir, and maribavir); 2) pathogenic dsDNA viruses; 3) in vitro activity. Read More

Arch Virol 2019 Jan 21. Epub 2019 Jan 21.

Department of Molecular Medicine, School of Medicine, University of Colima, Avenue Universidad 333, Colonia Las Víboras, CP 28040, Colima, Mexico.

Adenovirus 5 (Ad-5) infection is a common cause of acute respiratory infections and the main vector used in gene therapy. There are few studies on the relationship of Ad-5 to obesity. In the present study, we evaluated the chronic effects of Ad-5 infection on golden (Syrian) hamsters fed either a balanced diet (BD) or a high-fat diet (HFD). Read More

J Clin Virol 2019 Jan 11;112:1-9. Epub 2019 Jan 11.

Department of Ophthalmology, Faculty of Medicine and Graduate School of Medicine, Hokkaido University, Sapporo, Japan; Department of Ophthalmology, Health Sciences University of Hokkaido, Sapporo, Japan. Electronic address:

Adenoviral epidemic keratoconjunctivitis (EKC) presents as severe conjunctival inflammations involving the cornea that can lead to the development of corneal opacities and blurred vision, which can persist for months. EKC is highly contagious and responsible for outbreaks worldwide, therefore accurate diagnosis and rapid containment are imperative. EKC is caused by a number of types within Human adenovirus species D (HAdV-D): 8, 37 and 64 (formerly known as 19a) and these types were considered the major causes of EKC for over fifty years. Read More

Int J Infect Dis 2019 Jan 9;80:66-72. Epub 2019 Jan 9.

Istituto Zooprofilattico Sperimentale della Sicilia, Palermo, Italy.

Objectives: Enteric viruses are responsible for foodborne and waterborne infections affecting a large number of people. Data on food and water viral contamination in the south of Italy (Sicily) are scarce and fragmentary. The aim of this study was to evaluate the presence of viral contamination in food, water samples, and surface swabs collected in Sicily METHODS: The survey was conducted on 108 shellfish, 23 water samples (seawater, pipe water, and torrent water), 52 vegetables, one peach and 17 berries, 11 gastronomic preparations containing fish products and/or raw vegetables, and 28 surface swabs. Read More

Braz J Microbiol 2019 Jan 30;50(1):133-137. Epub 2018 Nov 30.

Medicine Department, Clinical Virology Laboratory, Infectious Diseases Unit, Sao Paulo Federal University, Rua Pedro de Toledo, 781, 15 andar, Sao Paulo, Brazil.

In 2007, the new polyomaviruses WUPyV and KIPyV were identified in patients with acute respiratory infections. The aim of this study was to investigate these viruses in hospitalized patients with severe acute respiratory infection (SARI). A retrospective study was conducted with 251 patients, from April 2009 to November 2010, using nasopharyngeal aspirates, naso- and oropharyngeal swab samples from hospitalized patients (children < 12 years and adults) who had SARI within 7 days of the onset of symptoms, including fever (> 38. Read More

Indian J Pediatr 2019 Jan 14. Epub 2019 Jan 14.

Department of Pediatrics, T.N. Medical College & B.Y.L. Nair Hospital, Mumbai, India.

Objective: To determine the frequency of respiratory pathogens in infants diagnosed with acute lower respiratory tract infections.

Methods: A prospective cross-sectional observational study was conducted in infants hospitalized with a diagnosis of acute lower respiratory tract infection (ALRTI), in a tertiary care hospital in a metropolitan city of Western India. Nasopharyngeal swabs were analyzed by multiplex real time polymerase chain reaction, for 18 viruses and 3 bacteria (H. Read More

J Med Virol 2019 Jan 13. Epub 2019 Jan 13.

Virology Laboratory, Infectious Diseases Division, Hospital de Clinicas, Universidade Federal do Parana, Curitiba, Brazil.

Acute gastroenteritis (AGE) remains a global public health concern and Nigeria is one of the two countries accounting for 42% of global under-5 deaths attributable to gastroenteritis. This study aimed to determine the prevalence, seasonality, and risk factors of enteric viruses (EVs) in children with AGE in Ogun State, Nigeria. Stool samples collected from children under-5 from three different hospitals between February 2015 and April 2017 were analyzed using molecular methods for the presence of four EVs (group A rotavirus [RVA], norovirus [NoV], human astrovirus [HAstV], and human adenovirus [HAdV]). Read More

Zhonghua Er Ke Za Zhi 2019 Jan;57(1):27-32

Beijing Key Laboratory of Pediatric Respiratory Infectious Diseases, Key Laboratory of Major Diseases in Children, Ministry of Education, National Clinical Research Center for Respiratory Diseases, National Key Discipline of Pediatrics (Capital Medical University), Beijing Pediatric Research Institute, Beijing Children's Hospital, Capital Medical University, National Center for Children's Health, Beijing 100045, China.

To investigate the predominant genotypes and epidemiological characteristics of human adenovirus (HAdV) in pediatric community-acquired pneumonia (CAP) in China. This was a repeated cross sectional study. Between November 2014 and November 2016, nasopharyngeal aspirates (NPAs) or throat swabs from each hospitalized pediatric patients diagnosed as CAP in 12 hospitals in Northern and Southern China were collected. Read More

BMC Infect Dis 2019 Jan 9;19(1):36. Epub 2019 Jan 9.

Department of Respiratory Medicine, Children's Hospital of Chongqing Medical University, Chongqing, 400014, China.

Background: Human adenovirus type 3 (HAdV-3) and 7 (HAdV-7) cause significant morbidity and develop severe complications and long-term pulmonary sequelae in children. However, epidemiologic reports have suggested that nearly all highly severe or fatal adenoviral diseases in children are associated with HAdV-7 rather than HAdV-3. Here, we conduct in-depth investigations to confirm and extend these findings through a comprehensive series of assays in vitro and in vivo as well as clinical correlates. Read More

JACC Basic Transl Sci 2018 Dec 31;3(6):728-740. Epub 2018 Dec 31.

Department of Medicine/Cardiology, University of Washington, Seattle, Washington.

A novel myosin heavy chain 7 mutation (E848G) identified in a familial cardiomyopathy was studied in patient-specific induced pluripotent stem cell-derived cardiomyocytes. The cardiomyopathic human induced pluripotent stem cell-derived cardiomyocytes exhibited reduced contractile function as single cells and engineered heart tissues, and genome-edited isogenic cells confirmed the pathogenic nature of the E848G mutation. Reduced contractility may result from impaired interaction between myosin heavy chain 7 and cardiac myosin binding protein C. Read More

Health Sci Rep 2018 Aug 18;1(8):e59. Epub 2018 Jul 18.

National Institute for Communicable Diseases of the National Health Laboratory Service Centre for Respiratory Diseases and Meningitis Johannesburg South Africa.

Aim: To investigate the prevalence of human bocavirus (hBoV), human coronaviruses (hCoV), and human polyomaviruses (hPyV) among patients with severe acute respiratory illness (SARI), in South Africa.

Methods: The study included 680 South African patients randomly selected in age-defined categories from hospitalised patients enrolled through SARI surveillance during 2012 to 2013. A multiplex reverse transcription real-time polymerase chain reaction assay was used to detect hBoV; hCoV-OC43, hCoV-229E, hCoV-NL63, and hCoV-HKU1; and Washington University hPyV (hPyV-WU) and Karolinska Insitute hPyV (hPyV-KI), in respiratory tract specimens collected from patients with SARI. Read More

BMC Ophthalmol 2019 Jan 7;19(1). Epub 2019 Jan 7.

Department of Ophthalmology, Faculty of Medicine, Tottori University, 36-1 Nishicho Yonago City, Tottori, Japan.

Background: Of the 10 patients with adenoviral type 54 keratoconjunctivitis examined at Nojima Hospital, 2 developed stellate keratitis and mutton-fat keratic precipitates (KPs) following acute symptoms.

Case Presentation: We encountered 10 cases of epidemic keratoconjunctivitis from August to October 2017. All patients were adults with a mean age of 60. Read More

J Med Virol 2019 Jan 4. Epub 2019 Jan 4.

Laboratório de Saneamento, Universidade Federal do Espírito Santo, Vitória, Espírito Santo, Brazil.

Acute gastroenteritis (AGE) is a significant cause of child mortality worldwide. In Brazil, despite the reduction in infant mortality achieved in recent years, many children still die because of undiagnosed AGE. The prevalence, viral load, and circulating genotypes of rotavirus A (RVA), human adenovirus (HAdV), and norovirus GII (NoV GII) were investigated in children with AGE during 12 months in Vitoria, Espírito Santo, Southeastern Brazil. Read More

J Clin Virol 2019 Feb 18;111:19-23. Epub 2018 Dec 18.

Department of Medical Microbiology, St. Olavs Hospital, Trondheim University Hospital, Norway; Department of Clinical and Molecular Medicine, Norwegian University of Science and Technology, Norway.

Background: Human adenovirus (HAdV) is a double-stranded DNA virus associated with respiratory tract infections (RTI) in children. Using polymerase chain reaction (PCR) tests, HAdV often is detected together with other virus species, even in healthy controls.

Objectives: The aim of this study was to compare molecular detection of HAdV with culture, and to examine the associations of various methods to RTI. Read More

J Infect Public Health 2018 Dec 17. Epub 2018 Dec 17.

National Institute of Public Health Research (INSPI), Guayaquil, Ecuador; Universidad Agraria del Ecuador, Guayaquil, Ecuador.

Background: Respiratory viral infections (RVI) are a leading cause of mortality worldwide. We compared the epidemiology and severity of RVI in Ecuador during 2009-2016.

Methods: Respiratory specimens collected within the national surveillance system were tested for influenza viruses, respiratory syncytial virus (RSV), adenovirus, parainfluenza virus, and human metapneumovirus. Read More

Rev Chil Pediatr 2018 Aug;89(4):521-524

Division of Pediatric Gastroenterology, Hepatology and Nutrition, Miller School of Medicine, University of Miami, USA.

Introduction: Scarlet fever is a common illness in pediatrics caused by group A beta-hemolytic strep tococcus (GABHS), which usually occurs after an episode of pharyngitis, and has an overall excellent prognosis. Hepatitis secondary to scarlet fever is a rare complication described in adults and even less frequently in children. Our objective was to describe a case of hepatitis secondary to scarlet fever in a pediatric patient. Read More

PLoS One 2018 14;13(12):e0209038. Epub 2018 Dec 14.

Department of Clinical Microbiology, Virology, and Laboratory for Molecular Infection Medicine Sweden (MIMS), Umeå University, Umeå, Sweden.

Human adenoviruses (HAdVs) are common pathogens that can cause respiratory, gastrointestinal, urogenital, and ocular infections. They are divided into seven species containing 85 genotypes. Straightforward typing systems might help epidemiological investigations. Read More

Rev Med Virol 2018 Dec 12:e2024. Epub 2018 Dec 12.

Department of Bioinformatics and Biotechnology, International Islamic University, Islamabad, Pakistan.

Respiratory infections, especially those of the lower respiratory tract, remain a foremost cause of mortality and morbidity of children greater than 5 years in developing countries including Pakistan. Ignoring these acute-level infections may lead to complications. Particularly in Pakistan, respiratory infections account for 20% to 30% of all deaths of children. Read More

Front Microbiol 2018 27;9:2919. Epub 2018 Nov 27.

Department of Microbiology, Public Health College, Harbin Medical University, Harbin, China.

Respiratory infections pose a great challenge in global health, and the prevalence of viral infection in adult patients has been poorly understood in northeast China. Harbin is one of the major cities in northeast China, and more than half of any given year in Harbin is occupied by winter. To reveal the viral etiology and seasonality in adult patients from Harbin, a 4-year consecutive survey was conducted in Harbin, China. Read More

Front Cell Infect Microbiol 2018 20;8:408. Epub 2018 Nov 20.

Division of Infectious Diseases, Department of Medicine, Duke University School of Medicine, Durham, NC, United States.

Human adenovirus (HAdV) infections are well-described after hematopoietic stem cell transplantation but less well understood in solid organ transplantation (SOT). We describe a case of disseminated HAdV type 21 infection 5 months after combined liver-kidney transplantation, expanding the limited literature describing this infection in the SOT population. Read More

PLoS Pathog 2018 12 10;14(12):e1007481. Epub 2018 Dec 10.

Heinrich Pette Institute, Leibniz Institute for Experimental Virology, Hamburg, Germany.

Cytomegaloviruses (CMVs) have a highly restricted host range as they replicate only in cells of their own or closely related species. To date, the molecular mechanisms underlying the CMV host restriction remain poorly understood. However, it has been shown that mouse cytomegalovirus (MCMV) can be adapted to human cells and that adaptation goes along with adaptive mutations in several viral genes. Read More

BMC Infect Dis 2018 Dec 7;18(1):634. Epub 2018 Dec 7.

Beijing Key Laboratory of Pediatric Respiratory Infection Diseases, Key Laboratory of Major Diseases in Children, Ministry of Education, National Clinical Research Center for Respiratory Diseases, National Key Discipline of Pediatrics (Capital Medical University), Beijing Pediatric Research Institute, Beijing Children's Hospital, Capital Medical University, National Center for Children's Health, Beijing, China.

Background: In adults, the emerging human adenovirus (HAdV) type 55 (HAdV-55) has been reported to cause more severe cases of adenovirus induced acute lower respiratory tract infections (ALRTIs) compared to other HAdV serotypes (HAdV-3, HAdV-7, HAdV-14). However, there is a dearth of comparative studies in children that address differences in the clinical epidemiological features between HAdV-55 and other HAdV serotypes that can also induce severe infection (such as HAdV-7).

Methods: We conducted a retrospective review of pediatric patients hospitalized at Beijing Children's Hospital with ALRTI from April 2008 to December 2013 who had adenovirus detected from nasopharyngeal or throat samples by PCR. Read More

Emerg Microbes Infect 2018 Dec 5;7(1):200. Epub 2018 Dec 5.

Beijing Institute of Biotechnology, 20 East Street, Beijing, 100071, China.

An apparent increase in the frequency of human adenovirus type 4 (HAdV-4) infections among general populations has been observed over the past 10 years. However, available epidemiological data that may reflect previous viral circulation and assist in predicting potential outbreaks are sparse, particularly in mainland China and Africa. In this study, a convenient neutralization assay for use in the surveillance of historical HAdV-4 infections was established based on a recombinant luciferase-expressing virus. Read More

Epigenetics 2018 Nov 21. Epub 2018 Nov 21.

a Institute for Virology, Friedrich-Alexander University Erlangen-Nürnberg , D-91054 Erlangen , Germany.

Apart from its well-documented role in long-term promoter silencing, the genome-wide distribution patterns of ~28 million methylated or unmethylated CpG dinucleotides, e. g. in the human genome, is in search of genetic functions. Read More

J Virol 2019 Feb 17;93(3). Epub 2019 Jan 17.

Department of Medical Biochemistry and Microbiology, Uppsala University, Uppsala, Sweden

Human adenovirus (HAdV) encodes a multifunctional DNA-binding protein pVII, which is involved in virus DNA packaging and extracellular immune signaling regulation. Although the pVII is an essential viral protein, its exact role in the virus life cycle and interplay with cellular proteins have remained to a large extent unclear. We have recently identified the cellular zinc finger protein 622 (ZNF622) as a potential pVII-interacting protein. Read More

J Biol Chem 2019 Jan 7;294(1):182-194. Epub 2018 Nov 7.

From the State Key Laboratory of Virology, Modern Virology Research Center, College of Life Sciences,

Viral infections still threaten human health all over the world, and many people die from viral diseases every year. However, there are no effective vaccines or drugs for preventing or managing most viral diseases. Thus, the discovery and development of broad-spectrum antiviral agents remain urgent. Read More

J Clin Virol 2018 Dec 1;109:35-40. Epub 2018 Nov 1.

Adolfo Lutz Institute, Virology Centre, Enteric Diseases Laboratory, São Paulo, SP, Brazil. Electronic address:

Background: In Brazil, limited data are available regarding the incidence and molecular epidemiology of Human Adenovirus (HAdV) in diarrheic disease, especially in the post rotavirus (RVA) vaccine era.

Objective: The aims of the study were to investigate the frequency of HAdV infections in patients with gastroenteritis during a 6-year period (2012-2017); conduct molecular typing of positive strains, and obtain further information on the HAdV seasonality.

Study Design: A total of 3003 fecal samples negative for both, RVA and Norovirus, were selected and tested for HAdV by PCR. Read More

Infect Genet Evol 2019 Jan 2;67:73-77. Epub 2018 Nov 2.

Department of Microbiology, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand; Center of Excellence in Emerging and Re-emerging Diarrheal Viruses, Chiang Mai University, Chiang Mai, Thailand. Electronic address:

Pigs have been assumed as a source of human viral infections. Surveillance of viruses in animals is essential to evaluate the risk to human and animal health and to determine economic impact. A number of studies focused mainly on well- known enteritis viruses such as porcine epidemic diarrhea virus (PEDV), transmissible gastroenteritis virus (TGEV), and porcine group A rotavirus (PRVA), however, little data is available for porcine adenovirus (PAdV). Read More

PLoS One 2018 26;13(10):e0202352. Epub 2018 Oct 26.

Institute of Virology and Microbiology, Centre for Biomedical Education and Research (ZBAF), School of Human Medicine, Faculty of Health, Witten/Herdecke University, Witten, Germany.

More than 70 human adenovirus types were identified divided into 7 different species (A-G). Diseases caused by human adenoviruses are type-dependent and can range from mild to severe respiratory infections, gastrointestinal infections or eye infections such as epidemic keratoconjunctivitis. Unfortunately there is no specific anti-adenovirus therapy available. Read More

BMC Genomics 2018 Oct 25;19(1):773. Epub 2018 Oct 25.

Key Laboratory for Medical Virology, National Health and Family Planning Commission, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Changping District, Beijing, 102206, China.

Background: Numerous protocols for viral enrichment and genome amplification have been created. However, the direct identification of viral genomes from clinical specimens using next-generation sequencing (NGS) still has its challenges. As a selected viral nucleic acid extraction method may determine the sensitivity and reliability of NGS, it is still valuable to evaluate the extraction efficiency of different extraction kits using clinical specimens directly. Read More

BMC Infect Dis 2018 Oct 22;18(1):529. Epub 2018 Oct 22.

Infection, Immunity and Inflammation, University of Leicester, Leicester, UK.

Background: Adenoviruses (AdV) are non-enveloped, double-stranded DNA viruses with multiple serotypes, which cause a variety of end-organ disease in both immunocompetent and immunocompromised individuals. Some adenoviruses can become latent in the mucosa-associated lymphoid tissue (e.g. Read More

Viruses 2018 10 18;10(10). Epub 2018 Oct 18.

Guangdong Provincial Key Laboratory of Tropical Disease Research, School of Public Health, Southern Medical University, Guangzhou 510515, China.

In 1955, Human adenovirus type 14 (HAdV-B14p) was firstly identified in a military trainee diagnosed as acute respiratory disease (ARD) in the Netherlands. Fifty years later, a genomic variant, HAdV-B14p1, re-emerged in the U.S. Read More

Clin Lab 2018 Oct;64(10):1777-1781

Background: The aim of this study was to determine the sensitivity and specificity of a novel immunochromatographic assay (ICA) kit, ALSONIC® Adeno (Alfresa Pharma Co., Osaka, Japan), for the detection of human adenovirus (HAdV) from throat swab samples based on the results of real-time PCR. The incubation time required for the novel assay kit (5 minutes) is shorter than that required for other ICA kits that are available in Japan. Read More

Clin Infect Dis 2018 Oct 17. Epub 2018 Oct 17.

Division of Gastrointestinal Sciences, Christian Medical College, Vellore, Tamil Nadu, India.

Background: Rotavirus vaccine (RV) efficacy estimates in low-resource settings are lower than in developed countries. We detected co-infections in cases of severe rotavirus diarrhea in a RV efficacy trial to determine whether these negatively impacted RV efficacy estimates.

Methods: We performed TaqMan Array Card assays for enteropathogens on stools from rotavirus EIA positive diarrhea episodes and all severe episodes (Vesikari score >= 11), from a phase III vaccine efficacy trial of Rotavac®, a monovalent human-bovine (116E) rotavirus vaccine, carried out across three sites in India. Read More

Emerg Infect Dis 2018 11;24(11):2117-2119

Among students with influenza-like illness at a Pennsylvania college student health center during 2016-2017, 44 (15%) of 288 with respiratory specimens tested positive for human adenovirus (HAdV). HAdV-3, -7, and -4 predominated, and types clustered temporally. HAdV infection should be considered among college students with acute respiratory illness. Read More

Acta Clin Belg 2018 Oct 11:1-7. Epub 2018 Oct 11.

a Department of Laboratory Medicine, Medical Microbiology , Ghent University Hospital , Ghent , Belgium.

Objectives: The Seegene Allplex Respiratory panel was retrospectively challenged using a collection of quality control samples (QCMD) and clinical samples previously analysed with validated routine methods.

Methods: A collection of 111 samples [43 QCMD samples, 13 bronchoalveolar lavage fluids and 55 nasopharyngeal aspirates/swabs] was tested with Seegene Allplex. The clinical samples were tested previously using either FTD® Respiratory Pathogens 21 qPCR assay (Fast Track Diagnostics), an in-house multiplex PCR for Bordetella, or BioGX Sample-Ready Atypical pneumo panel (Becton Dickinson). Read More

Antiviral Res 2018 Nov 1;159:122-129. Epub 2018 Oct 1.

Department of Pediatrics, University of Alabama School of Medicine, Birmingham, AL, 35233, USA. Electronic address:

The search for new compounds with a broad spectrum of antiviral activity is important and requires the evaluation of many compounds against several distinct viruses. Researchers attempting to develop new antiviral therapies for DNA virus infections currently use a variety of cell lines, assay conditions and measurement methods to determine in vitro drug efficacy, making it difficult to compare results from within the same laboratory as well as between laboratories. In this paper, we describe the assessment of antiviral activity of a set of nucleoside analogs against BK polyomavirus, JC polyomavirus, Epstein-Barr virus, human herpesvirus 6B, and human herpesvirus 8 in an automated 384-well format and utilize qPCR assays to measure the accumulation of viral DNA. Read More

Antiviral Res 2018 Nov 1;159:104-112. Epub 2018 Oct 1.

Department of Pediatrics, University of Alabama School of Medicine, Birmingham, AL, 35233, United States. Electronic address:

The search for new compounds with a broad spectrum of antiviral activity is important and requires the evaluation of many compounds against several distinct viruses. Researchers attempting to develop new antiviral therapies for DNA virus infections currently use a variety of cell lines, assay conditions and measurement methods to determine in vitro drug efficacy, making it difficult to compare results from within the same laboratory as well as between laboratories. In this paper we describe a common assay platform designed to facilitate the parallel evaluation of antiviral activity against herpes simplex virus type 1, herpes simplex virus type 2, varicella-zoster virus, cytomegalovirus, vaccinia virus, cowpox virus, and adenovirus. Read More

J Med Virol 2019 Mar 28;91(3):392-400. Epub 2018 Oct 28.

Hunan Provincial People's Hospital, Children's Medical Center, Changsha, China.

To investigate the current genotypes of circulating human adenovirus (HAdV) strains, we molecularly genotyped HAdV in the nasopharyngeal aspirates (NPAs) of patients with acute lower respiratory tract infections (ALRTIs) and attempted to determine their associations with clinical symptoms. A total of 4751 NPA samples were collected from 4751 patients admitted to Hunan Provincial People's Hospital from September 2007 to March 2014, of which 447 (9.4%) samples were HAdV positive. Read More