24 results match your criteria Pfluegers Archiv : European journal of physiology[Journal]

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Acute exercise activates nuclear factor (NF)-kappaB signaling pathway in rat skeletal muscle.

FASEB J 2004 Oct;18(13):1499-506

Department of Kinesiology, University of Wisconsin, Madison, Wisconsin, USA.

Two studies were performed to investigate the effects of an acute bout of physical exercise on the nuclear protein kappaB (NF-kappaB) signaling pathway in rat skeletal muscle. In Study 1, a group of rats (n=6) was run on the treadmill at 25 m/min, 5% grade, for 1 h or until exhaustion (Ex), and compared with a second group (n=6) injected with two doses of pyrrolidine dithiocarbamate (PDTC, 100 mg/kg, i.p. Read More

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October 2004
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Characteristics of a low affinity passive Ca2+ influx component in rat parotid gland basolateral plasma membrane vesicles.

J Membr Biol 1998 Mar;162(2):139-45

Secretory Physiology Section, Gene Therapy and Therapeutics Branch, National Institute of Dental Research, National Institutes of Health, Bethesda, Maryland 20892, USA.

We have previously reported the presence of two Ca2+ influx components with relatively high (KCa = 152 +/- 79 microM) and low (KCa = 2.4 +/- 0.9 mM) affinities for Ca2+ in internal Ca2+ pool-depleted rat parotid acinar cells [Chauthaiwale et al. Read More

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cAMP modulation of a Ca2+-dependent K+ conductance in rat submandibular acinar cells.

Authors:
T Ishikawa

Am J Physiol 1997 Mar;272(3 Pt 1):G454-62

Department of Molecular Physiology, National Institute for Physiological Sciences, Okazaki, Japan.

Rat submandibular acinar cells exhibit a tetraethylammonium (TEA)-insensitive, Ca2+-activated K+ conductance, which may play an important role in Ca2+-dependent fluid secretion by this gland (T. Ishikawa, M. Murakami, and Y. Read More

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Identification of a cDNA/protein leading to an increased Pi-uptake in Xenopus laevis oocytes.

J Membr Biol 1997 Mar;156(1):19-24

Institute of Physiology, University of Zürich, Switzerland.

In a previous report we documented an increased Na(+)-dependent transport of inorganic phosphate (P(i)) in Xenopus laevis oocytes injected with mRNA isolated from rabbit duodenum (Yagci et al., Pfluegers Arch. 422:211-216, 1992; ref 24). Read More

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cGMP-activating peptides do not regulate electrogenic electrolyte transport in principal cells of rat CCD.

Am J Physiol 1996 Dec;271(6 Pt 2):F1158-65

Westfälische Wilhelms-Universität Münster, Germany.

K+ channels in the basolateral membrane of rat cortical collecting duct (CCD) are regulated by a cGMP-dependent protein kinase (J. Hirsch and E. Schlatter. Read More

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December 1996

Analysis of respiratory water--a new method for evaluation of myocardial energy metabolism.

J Appl Physiol (1985) 1996 Nov;81(5):2115-22

Institute of Experimental Surgery, Heinrich Heine University, Düsseldorf, Germany.

Aerobic ATP synthesis via oxidative phosphorylation causes a proportional production of respiratory water. Thus the amount of respiratory water produced at a given time should be a reliable measure of the current ATP demand of the mammalian myocardium. Respiratory water from isolated rabbit hearts was labeled by using the stable oxygen isotope 18O. Read More

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November 1996

Gated, ion-selective channels observed with patch pipettes in the absence of membranes: novel properties of a gigaseal.

Authors:
F Sachs F Qin

Biophys J 1993 Sep;65(3):1101-7

State University of New York, Buffalo 14124.

Gigaohm seals made between patch pipettes and hydrophobic substrates have a finite conductance which are cation-selective and capable of producing quantized gating indistinguishable from the gating of biological ion channels. The selectivity sequence and streaming potentials of these seals suggests the existence of a pore of similar dimensions to the nicotinic acetylcholine channel. The ionic selectivity of these seals appears similar to the seal selectivity observed with membrane patches (Fischmeister, R. Read More

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September 1993

Acetylcholine activates nonselective cation channels in guinea pig ileum through a G protein.

Authors:
R Inoue G Isenberg

Am J Physiol 1990 Jun;258(6 Pt 1):C1173-8

Department of Physiology, University of Cologne, Federal Republic of Germany.

Acetylcholine (ACh) depolarizes the membrane of mammalian intestinal myocytes by activating a nonselective cation channel (G. D. Benham, T. Read More

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Vasopressin and mineralocorticoid increase apical membrane driving force for K+ secretion in rat CCD.

Am J Physiol 1990 Jan;258(1 Pt 2):F199-210

Department of Physiology, University of Alabama, Birmingham 35294.

Cortical collecting ducts (CCD) from untreated Sprague-Dawley rats were perfused and bathed in vitro with modified Krebs-Ringer solutions. Arginine vasopressin (AVP;100 microU/ml) in the bathing solution hyperpolarized the transepithelial voltage (PDT, mV) from -2.3 +/- 0. Read More

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January 1990

D 600 block of L-type Ca2+ channel in vascular smooth muscle cells: comparison with permanently charged derivative, D 890.

Authors:
N Leblanc J R Hume

Am J Physiol 1989 Oct;257(4 Pt 1):C689-95

Department of Physiology, University of Nevada School of Medicine, Reno 89557-0046.

It has been reported that D 600 blocks the high-threshold Ca2+ channel (L-type) from the outside in isolated vascular and ileal smooth muscle cells of the rabbit (Y. Ohya, K. Terada, K. Read More

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October 1989

Energy dependence of enzyme release from hypoxic isolated perfused rat heart tissue.

J Appl Physiol (1985) 1988 Oct;65(4):1855-60

Division of Pharmacology, College of Pharmacy, University of Texas, Austin 78712.

There is a sudden release of intracellular constituents upon reoxygenation of isolated perfused hypoxic heart tissue (O2 paradox) or on perfusion with calcium-free medium after a period of hypoxia. Rat hearts were perfused by the method of Langendorff (Pfluegers Arch. 61: 291-332, 1895) with Krebs-Henseleit medium containing 10 mM glucose. Read More

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October 1988

Diphenylamine-2-carboxylate blocks Cl(-)-HCO3- exchange in Necturus gallbladder epithelium.

Am J Physiol 1987 Jul;253(1 Pt 1):C79-89

Intracellular microelectrode techniques were employed to study the effects of diphenylamine-2-carboxylate (DPC) on ion transport in Necturus gallbladder epithelium. Under control conditions, addition of DPC to the mucosal bathing solution caused a concentration-dependent, reversible hyperpolarization of both cell membranes with no measurable resistance changes. In addition, DPC caused the following effects, all consistent with inhibition of apical membrane Cl(-)-HCO3- exchange: fall in intracellular Cl- activity (aCli), increase in intracellular pH (pHi), reduction of the changes in aCli and pHi produced by lowering mucosal solution [Cl-], and reduction of the change in pHi produced by lowering mucosal solution [HCO3-]. Read More

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Supercharging: a method for improving patch-clamp performance.

Biophys J 1987 Jul;52(1):133-6

Patch-clamp performance can be improved without altering the normal headstage configuration described by (Hamill, O. P., A. Read More

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A uniform enzymatic method for dissociation of myocytes from hearts and stomachs of vertebrates.

Authors:
R Mitra M Morad

Am J Physiol 1985 Nov;249(5 Pt 2):H1056-60

A method is presented that consistently yields a large number of calcium-tolerant myocytes from mammalian, amphibian, and elasmobranch hearts and from mammalian stomach. The use of incubating solutions or cell harvesting techniques was not required. The time needed to isolate cells was shorter than previously reported values. Read More

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November 1985

Potentiometric measurements of stoichiometric and apparent affinity constants of EGTA for protons and divalent ions including calcium.

Biochim Biophys Acta 1985 May;839(3):287-99

The stoichiometric affinity constants of H+, Ca2+, Mg2+ and Sr+ for the ligand EGTA were determined using a modified version of the pH metric method developed by Moisescu and Pusch (Moisescu, D.G. and Pusch, H. Read More

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Single voltage-dependent chloride-selective channels of large conductance in cultured rat muscle.

Biophys J 1983 Aug;43(2):237-41

Single-channel currents of an anion-selective channel in the plasma membrane of cultured rat muscle cells (myotubes) were recorded with the patch-clamp technique (Hamill, O.P., A. Read More

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The metabolism and hormonal responses of human eccrine sweat glands isolated by collagenase digestion.

Authors:
T Kealey

Biochem J 1983 Apr;212(1):143-8

1. Collagenase digestion of biopsies of human skin yields eccrine sweat glands that can be picked out under binocular light microscopy. The glands are viable as determined by the exclusion of Trypan Blue, the uptake of Methylene Blue, electron microscopy, the rate of lactate dehydrogenase release, ATP content and the rates of glucose oxidation and lactate release. Read More

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The influence of amino-reactive substances on contraction threshold of frog skeletal muscle.

J Membr Biol 1983 ;73(1):17-23

The action of the amino-reactive substances pyridoxal phosphate, 4-acetamido-4'-isothiocyanato-stilbene-2,2'-disulfonic acid and 2,4,6-trinitrobenzene sulfonic acid on the contraction threshold, taken as parameter for the initiation of contraction, was investigated in fibers of the sartorius muscle of the frog. The contraction threshold was shifted by 1 to 11 mV to more negative potentials with 1 to 20 mM PDP. Similar shifts from 2 to 17 mV were produced by 0. Read More

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Influence of chloride, potassium, and tetraethylammonium on the early outward current of sheep cardiac Purkinje fibers.

J Gen Physiol 1979 Feb;73(2):117-38

In voltage clamp studies of cardiac Purkinje fibers, a large early outward current is consistently observed during depolarizations to voltages more positive than -20 mV. After the outward peak of the current, the total membrane current declines slowly. Dudel et al. Read More

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February 1979

The influence of intercellular clefts on the electrical properties of sheep cardiac Purkinje fibers.

Biophys J 1979 Feb;25(2 Pt 1):217-34

A model of a 100 micrometers diameter Purkinje fiber with intercellular clefts was studied under voltage clamp conditions to examine the consequences of radial nonuniformity. Sodium and potassium conductances were distributed so that the surface and cleft membranes had similar channel density. Assuming that the model is appropriate, sodium current (and conductance) measured in the voltage clamp is grossly underestimated because of loss of voltage control of the cleft membrane. Read More

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February 1979

Influence of lithium upon the intracellular potential of frog skin epithelium.

Authors:
W Nagel

J Membr Biol 1977 Dec;37(3-4):347-59

The effect of Li upon the intracellular potential of frog skin (Rana esculenta) was investigated. In the range between 1 and 25 mM Li in the epithelial bathing solution, a semilogarithmic linear relationship between [Li] and intracellular potential under short circuit conditions was obtained. The intracellular potential at all [Li] is quantitatively sufficient to explain the previously reported accumulation of Li in the intracellular space of the frog skin epithelium (Leblanc, G. Read More

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December 1977

Proton fluxes associated with erythrocyte membrane anion exchange.

Authors:
M L Jennings

J Membr Biol 1976 Aug;28(2-3):187-205

Transient extracellular pH changes accompany the exchange of chloride for sulfate across the erythrocyte membrane. The direction of the extracellular pH change during chloride efflux and sulfate influx depends on experimental conditions. When bicarbonate is present, the extracellular pH drops sharply at the outset of the anion exchange and tends to follow the partial ionic equilibrium described by Wilbrandt (W. Read More

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