12,240 results match your criteria Pfluegers Archiv : European journal of physiology[Journal]


Role of K channels in maintaining the synchrony of spontaneous Ca transients in the mural cells of rat rectal submucosal arterioles.

Pflugers Arch 2019 Apr 13. Epub 2019 Apr 13.

Department of Cell Physiology, Nagoya City University Graduate School of Medical Sciences, Nagoya, Japan.

Mural cells in precapillary arterioles (PCAs) generate spontaneous Ca transients primarily arising from the periodic release of Ca from sarcoendoplasmic reticulum (SR/ER). The Ca release induces Ca-activated chloride channel (CaCC)-dependent depolarisations that spread to neighbouring mural cells to develop the synchrony of their Ca transients. Here, we explored the roles of K channels in maintaining the synchrony of spontaneous Ca transients. Read More

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http://dx.doi.org/10.1007/s00424-019-02274-3DOI Listing

Intermittent normobaric hypoxia facilitates high altitude acclimatization by curtailing hypoxia-induced inflammation and dyslipidemia.

Pflugers Arch 2019 Apr 13. Epub 2019 Apr 13.

Defence Research and Development Organisation, Defence Institute of Physiology and Allied Sciences (DIPAS), Lucknow Road, Timarpur, New Delhi, 110054, India.

Intermittent hypoxic training (IHT) is a discrete cost-effective method for improving athletic performance and high altitude acclimatization. Unfortunately, IHT protocols widely vary in terms of hypoxia severity, duration, and number of cycles affecting physiological outcomes. In the present study, we evaluated the efficacy of a moderate normobaric IHT protocol (12% FiO for 4 h, 4 days) on acclimatization to high altitude (3250 m). Read More

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http://dx.doi.org/10.1007/s00424-019-02273-4DOI Listing

Sarcomeric mutations in cardiac diseases.

Pflugers Arch 2019 Apr 11. Epub 2019 Apr 11.

Department of Physiology and Biophysics, College of Medicine, University of Illinois, 835 S. Wolcott Rm E202 (MC901), Chicago, IL, 60612-7342, USA.

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http://dx.doi.org/10.1007/s00424-019-02275-2DOI Listing

Titin mutations and muscle disease.

Pflugers Arch 2019 Mar 27. Epub 2019 Mar 27.

Department of Cellular and Molecular Medicine, University of Arizona, MRB 325. 1656 E Mabel Street, Tucson, AZ, 85724-5217, USA.

The introduction of next-generation sequencing technology has revealed that mutations in the gene that encodes titin (TTN) are linked to multiple skeletal and cardiac myopathies. The most prominent of these myopathies is dilated cardiomyopathy (DCM). Over 60 genes are linked to the etiology of DCM, but by far, the leading cause of DCM is mutations in TTN with truncating variants in TTN (TTNtvs) associated with familial DCM in ∼ 20% of the cases. Read More

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http://link.springer.com/10.1007/s00424-019-02272-5
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http://dx.doi.org/10.1007/s00424-019-02272-5DOI Listing
March 2019
8 Reads

Quantification of cardiac pumping mechanics in rats by using the elastance-resistance model based solely on the measured left ventricular pressure and cardiac output.

Pflugers Arch 2019 Mar 23. Epub 2019 Mar 23.

Department of Physiology, College of Medicine, National Taiwan University, No. 1, Sec. 1, Jen-Ai Road, Taipei, 100, Taiwan.

The cardiac pumping mechanics can be characterized by both the maximal systolic elastance (E) and theoretical maximum flow (Q), which are generated using an elastance-resistance model. The signals required to fit the elastance-resistance model are the simultaneously recorded left ventricular (LV) pressure and aortic flow (Q), followed by the isovolumic LV pressure. In this study, we evaluated a single-beat estimation technique for determining the E and Q by using the elastance-resistance model based solely on the measured LV pressure and cardiac output. Read More

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http://dx.doi.org/10.1007/s00424-019-02270-7DOI Listing

Normal increases in insulin-stimulated glucose uptake after ex vivo contraction in neuronal nitric oxide synthase mu (nNOSμ) knockout mice.

Pflugers Arch 2019 Mar 21. Epub 2019 Mar 21.

Institute of Health and Sport (IHeS), Victoria University, Melbourne, Australia.

Nitric oxide (NO) is involved in skeletal muscle glucose uptake during exercise and also in the increase in insulin sensitivity after exercise. Given that neuronal nitric oxide synthase (NOS) isoform mu (nNOSμ) is a major isoform of NOS in skeletal muscle, we examined if the increase in skeletal muscle insulin-stimulated glucose uptake 3.5 h following ex vivo contraction of extensor digitorum longus (EDL) is reduced in muscles from nNOSμ and nNOSμ mice compared with nNOSμ mice. Read More

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http://dx.doi.org/10.1007/s00424-019-02268-1DOI Listing

Receptor for advanced glycation end-products modulates lung development and lung sensitivity to hyperoxic injury in newborn mice.

Pflugers Arch 2019 Mar 16. Epub 2019 Mar 16.

Department of Cardiac Surgery, Middle German Heart Center, University Hospital Halle (Saale), Martin Luther University Halle-Wittenberg, Ernst-Grube-Str. 40, 06120, Halle (Saale), Germany.

The receptor for advanced glycation end-products is mainly expressed in type I alveolar epithelial cells but its importance in lung development and response to neonatal hyperoxia is unclear. Therefore, our study aimed at the analysis of young wildtype and RAGE knockout mice which grew up under normoxic or hyperoxic air conditions for the first 14 days followed by a longer period of normoxic conditions. Lung histology, expression of lung-specific proteins, and respiratory mechanics were analyzed when the mice reached an age of 2 or 4 months. Read More

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http://dx.doi.org/10.1007/s00424-019-02267-2DOI Listing

Sweet taste receptors as a tool for an amplifying pathway of glucose-stimulated insulin secretion in pancreatic β cells.

Pflugers Arch 2019 Apr 15;471(4):655-657. Epub 2019 Mar 15.

Department of Physiology & Obesity-mediated Disease Research Center, Keimyung University School of Medicine, 1095 Dalgubeoldaero, Dalseogu, Daegu, 42601, South Korea.

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http://dx.doi.org/10.1007/s00424-019-02271-6DOI Listing

Moving beyond simple answers to complex disorders in sarcomeric cardiomyopathies: the role of integrated systems.

Pflugers Arch 2019 Mar 8. Epub 2019 Mar 8.

Department of Biomedical Engineering, University of Arizona, Tucson, AZ, USA.

The classic clinical definition of hypertrophic cardiomyopathy (HCM) as originally described by Teare is deceptively simple, "left ventricular hypertrophy in the absence of any identifiable cause." Longitudinal studies, however, including a seminal study performed by Frank and Braunwald in 1968, clearly described the disorder much as we know it today, a complex, progressive, and highly variable cardiomyopathy affecting ~ 1/500 individuals worldwide. Subsequent genetic linkage studies in the early 1990s identified mutations in virtually all of the protein components of the cardiac sarcomere as the primary molecular cause of HCM. Read More

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http://dx.doi.org/10.1007/s00424-019-02269-0DOI Listing
March 2019
1 Read

Correction to: Four novel interaction partners demonstrate diverse modulatory effects on voltage-gated Ca2.2 Ca channels.

Pflugers Arch 2019 Feb 22. Epub 2019 Feb 22.

Center of Bioscience, Institute for Molecular Physiology and Genetics, 84005, Bratislava, Slovakia.

The article was originally published with one author missing. The name of the co-author Roman Moravcik was inadvertently omitted. His name and affiliation have now been added to the author list. Read More

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http://dx.doi.org/10.1007/s00424-019-02265-4DOI Listing
February 2019
1 Read
4.101 Impact Factor

Exercise physiology: future opportunities and challenges.

Pflugers Arch 2019 Mar;471(3):381

Section for Cell Biology and Physiology, Department of Biology, University of Copenhagen, Universitetsparken 13, 2100, Copenhagen Ø, Denmark.

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http://dx.doi.org/10.1007/s00424-019-02263-6DOI Listing
March 2019
1 Read

Three perspectives on the molecular basis of hypercontractility caused by hypertrophic cardiomyopathy mutations.

Authors:
James A Spudich

Pflugers Arch 2019 Feb 15. Epub 2019 Feb 15.

Department of Biochemistry, Stanford University School of Medicine, Stanford, CA, 94305, USA.

Several lines of evidence suggest that the primary effect of hypertrophic cardiomyopathy mutations in human β-cardiac myosin is hypercontractility of the heart, which leads to subsequent hypertrophy, fibrosis, and myofilament disarray. Here, I describe three perspectives on the molecular basis of this hypercontractility. The first is that hypercontractility results from changes in the fundamental parameters of the actin-activated β-cardiac myosin chemo-mechanical ATPase cycle. Read More

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http://dx.doi.org/10.1007/s00424-019-02259-2DOI Listing
February 2019
1 Read

The functional impact of G protein-coupled receptor 142 (Gpr142) on pancreatic β-cell in rodent.

Pflugers Arch 2019 Apr 15;471(4):633-645. Epub 2019 Feb 15.

Department of Clinical Science, SUS, Division of Islet Cell Physiology, University of Lund, Jan Waldenströmsgata 35, Building 91, Floor 11, SE-205 02, Malmö, Sweden.

We have recently shown that the G protein-coupled receptor 142 (GPR142) is expressed in both rodent and human pancreatic β-cells. Herein, we investigated the cellular distribution of GPR142 within islets and the effects of selective agonists of GPR142 on glucose-stimulated insulin secretion (GSIS) in the mouse islets and INS-1832/13 cells. Double-immunostaining revealed that GPR142 immunoreactivity in islets mainly occurs in insulin-positive cells. Read More

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http://dx.doi.org/10.1007/s00424-019-02262-7DOI Listing

Altered force generation and cell-to-cell contractile imbalance in hypertrophic cardiomyopathy.

Pflugers Arch 2019 Feb 11. Epub 2019 Feb 11.

Molecular and Cell Physiology, Hannover Medical School, Carl-Neuberg-Str. 1, 30625, Hannover, Germany.

Hypertrophic cardiomyopathy (HCM) is mainly caused by mutations in sarcomeric proteins. Thirty to forty percent of identified mutations are found in the ventricular myosin heavy chain (β-MyHC). A common mechanism explaining how numerous mutations in several different proteins induce a similar HCM-phenotype is unclear. Read More

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http://dx.doi.org/10.1007/s00424-019-02260-9DOI Listing
February 2019
1 Read

Deregulated renal magnesium transport during lipopolysaccharide-induced acute kidney injury in mice.

Pflugers Arch 2019 Apr 6;471(4):619-631. Epub 2019 Feb 6.

Institute of Experimental and Clinical Pharmacology and Toxicology, Friedrich-Alexander-Universität Erlangen-Nürnberg (FAU), Fahrstr. 17, 91054, Erlangen, Germany.

Magnesium (Mg) abnormalities during sepsis have been reported, but the underlying mechanisms during acute inflammation are poorly understood. We hypothesized that a decrease in GFR and/or changes in transporters or channels for Mg could be responsible for the observed Mg abnormalities. Therefore, we studied the metabolism of Mg in a murine model of endotoxemia. Read More

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http://dx.doi.org/10.1007/s00424-019-02261-8DOI Listing
April 2019
2 Reads

Mitochondrial dynamics in exercise physiology.

Pflugers Arch 2019 Feb 1. Epub 2019 Feb 1.

Division of Cardiocirculatory Signaling, National Institute for Physiological Sciences (NIPS), National Institutes of Natural Sciences, Aichi, 444-8787, Japan.

A growing body of evidence suggests that exercise shows pleiotropic effects on the maintenance of systemic homeostasis through mitochondria. Dysregulation of mitochondrial dynamism is associated with metabolic inflexibility, resulting in many of the metabolic diseases and aging. Studies have suggested that exercise prevents and delays the progression of mitochondrial dysfunction by improving mitochondrial metabolism, biogenesis, and quality control. Read More

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http://dx.doi.org/10.1007/s00424-019-02258-3DOI Listing
February 2019
1 Read

Hereditary heart disease: pathophysiology, clinical presentation, and animal models of HCM, RCM, and DCM associated with mutations in cardiac myosin light chains.

Pflugers Arch 2019 Jan 31. Epub 2019 Jan 31.

Department of Molecular and Cellular Pharmacology, University of Miami Miller School of Medicine, 1600 NW 10th Ave., Miami, FL, 33136, USA.

Genetic cardiomyopathies, a group of cardiovascular disorders based on ventricular morphology and function, are among the leading causes of morbidity and mortality worldwide. Such genetically driven forms of hypertrophic (HCM), dilated (DCM), and restrictive (RCM) cardiomyopathies are chronic, debilitating diseases that result from biomechanical defects in cardiac muscle contraction and frequently progress to heart failure (HF). Locus and allelic heterogeneity, as well as clinical variability combined with genetic and phenotypic overlap between different cardiomyopathies, have challenged proper clinical prognosis and provided an incentive for identification of pathogenic variants. Read More

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http://dx.doi.org/10.1007/s00424-019-02257-4DOI Listing
January 2019
2 Reads
4.101 Impact Factor

SGK1-dependent stimulation of vascular smooth muscle cell osteo-/chondrogenic transdifferentiation by interleukin-18.

Pflugers Arch 2019 Jan 31. Epub 2019 Jan 31.

Department of Internal Medicine and Cardiology, Charité - Universitätsmedizin Berlin, Campus Virchow-Klinikum, Augustenburgerplatz 1, 13353, Berlin, Germany.

The serum- and glucocorticoid-inducible kinase 1 (SGK1) is a key regulator of osteo-/chondrogenic transdifferentiation and subsequent calcification of vascular smooth muscle cells (VSMCs). The phenotypical transdifferentiation of VSMCs is associated with increased interleukin-18 (IL-18) levels and generalized inflammation. Therefore, the present study investigated the possible involvement of SGK1 in IL-18-induced vascular calcification. Read More

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http://link.springer.com/10.1007/s00424-019-02256-5
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http://dx.doi.org/10.1007/s00424-019-02256-5DOI Listing
January 2019
4 Reads

Renal ischemia-reperfusion injury impairs renal calcium, magnesium, and phosphate handling in mice.

Pflugers Arch 2019 Jan 26. Epub 2019 Jan 26.

Institute of Experimental and Clinical Pharmacology and Toxicology, Friedrich-Alexander-Universität Erlangen-Nürnberg (FAU), Fahrstr. 17, D-91054, Erlangen, Germany.

Fibroblast growth factor 23 (FGF23) levels are elevated in patients with acute kidney injury (AKI). The consequences on renal Ca, Mg, and P regulatory mechanisms are unknown. We hypothesized that renal ischemia-reperfusion (I/R) injury alters the expression of important renal Ca, Mg, and P transport proteins. Read More

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http://dx.doi.org/10.1007/s00424-019-02255-6DOI Listing
January 2019
5 Reads

Exercise training-induced changes in metabolic syndrome parameters, carotid wall thickness, and thyroid function in middle-aged women with subclinical hypothyroidism.

Pflugers Arch 2019 Mar 18;471(3):479-489. Epub 2019 Jan 18.

Department of Physical Education, College of Physical Education, Keimyung University, 1095 Dalgubeuldaero, Dalseo-gu, Daegu, 42601, South Korea.

This study analyzed the differences in effects of a 12-week combination of exercise training program with resistance training and aerobic exercises on the risk factors of metabolic syndrome, carotid wall thickness, and thyroid function, between subclinical hypothyroidism patients and obese groups, in middle-aged women. Subjects consisted of either 20 middle-aged women in the subclinical hypothyroidism (SCH) group or 20 obese (body mass indices [BMI], ≥ 25 kg/m) women without hypothyroidism in the obese (OB) group. The body composition, blood lipid factors, hormones associated with thyroid functions, blood pressure (BP), and carotid intima-media thickness were measured, while physical fitness was ascertained. Read More

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http://dx.doi.org/10.1007/s00424-019-02254-7DOI Listing
March 2019
6 Reads

Rab8a is involved in membrane trafficking of Kir6.2 in the MIN6 insulinoma cell line.

Pflugers Arch 2019 Jan 10. Epub 2019 Jan 10.

Department of Pharmacology, Faculty of Medicine, Saga University, Saga, 849-8501, Japan.

Although ATP-sensitive K (K) channels play an important role in the secretion of insulin by pancreatic beta cells, the mechanisms that regulate the intracellular transport of K channel subunit proteins (i.e., Kir6. Read More

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http://dx.doi.org/10.1007/s00424-018-02252-1DOI Listing
January 2019
2 Reads

Roles of myokines in exercise-induced improvement of neuropsychiatric function.

Pflugers Arch 2019 Mar 9;471(3):491-505. Epub 2019 Jan 9.

Department of Pharmacology and Hypoxia-related Disease Research Center, Inha University School of Medicine, Room 1015, 60th Anniversary Hall, 100, Inha-ro, Nam-gu, Incheon, 22212, Republic of Korea.

Exercise is a well-known non-pharmacological intervention to improve brain functions, including cognition, memory, and motor coordination. Contraction of skeletal muscles during exercise releases humoral factors that regulate the whole-body metabolism via interaction with other non-muscle organs. Myokines are muscle-derived effectors that regulate body metabolism by autocrine, paracrine, or endocrine action and were reportedly suggested as "exercise factors" that can improve the brain function. Read More

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http://link.springer.com/10.1007/s00424-019-02253-8
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http://dx.doi.org/10.1007/s00424-019-02253-8DOI Listing
March 2019
13 Reads

The exocrine pancreas is an extracardiac source of atrial natriuretic peptide.

Pflugers Arch 2019 Jan 8. Epub 2019 Jan 8.

CONICET-Instituto de Inmunología, Genética y Metabolismo (INIGEM), Universidad de Buenos Aires, Buenos Aires, Argentina.

Previous studies have shown that atrial natriuretic peptide (ANP) regulates exocrine pancreatic function in health and disease. As extracardiac sources of ANP have been identified and ANP-like immunoreactivity has been reported in the exocrine pancreas, in the present work we sought to establish whether ANP was produced in the rat exocrine pancreas and if conditions like fasting/feeding or acute pancreatitis were reflected on ANP expression. By using RT-PCR, immunoblotting, and immunofluorescence microscopy assays, it was found that both mRNA and protein ANP were present in the acinar cells of the exocrine pancreas. Read More

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http://dx.doi.org/10.1007/s00424-018-02247-yDOI Listing
January 2019
3 Reads

Ablation of catalase promotes non-alcoholic fatty liver via oxidative stress and mitochondrial dysfunction in diet-induced obese mice.

Pflugers Arch 2019 Jan 7. Epub 2019 Jan 7.

Department of Physiology & Obesity-mediated Disease Research Center, Keimyung University School of Medicine, 1095 Dalgubeoldae-Ro, Dalseo-Gu, Daegu, 42601, South Korea.

Hydrogen peroxide (HO) produced endogenously can cause mitochondrial dysfunction and metabolic complications in various cell types by inducing oxidative stress. In the liver, oxidative and endoplasmic reticulum (ER) stress affects the development of non-alcoholic fatty liver disease (NAFLD). Although a link between both stresses and fatty liver diseases has been suggested, few studies have investigated the involvement of catalase in fatty liver pathogenesis. Read More

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http://link.springer.com/10.1007/s00424-018-02250-3
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http://dx.doi.org/10.1007/s00424-018-02250-3DOI Listing
January 2019
8 Reads

Visualizing the regulation of SLC34 proteins at the apical membrane.

Pflugers Arch 2019 Apr 6;471(4):533-542. Epub 2019 Jan 6.

Department of Biomedical Engineering, Laboratory for Fluorescence Dynamics, University of California at Irvine, Irvine, CA, USA.

The cloning of the renal NaPi-2a (SLC34A1) and NaPi-2c (SLC34A3) phosphate transporters has made it possible to characterize the molecular and biophysical regulation of renal proximal tubular reabsorption of inorganic phosphate (Pi). Dietary factors, such as Pi and K, and several hormones and phosphatonins, including parathyroid hormone (PTH), fibroblast growth factor 23 (FGF23), and glucocorticoids, regulate the transporters through various transcriptional, translational, and post-translational mechanisms that involve acute trafficking via endocytosis or exocytosis, interactions with PDZ domain proteins, lipid microdomains, and diffusion and clustering in the apical brush border membrane. The visualization of these trafficking events by means of novel microscopy techniques that includes fluorescence lifetime imaging microscopy (FLIM), Förster resonance energy transfer (FRET), fluctuation correlation spectroscopy (FCS), and modulation tracking (MT), is the primary focus of this review. Read More

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http://dx.doi.org/10.1007/s00424-018-02249-wDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6436987PMC
April 2019
1 Read

Protective efficacy of dinitrosyl iron complexes with reduced glutathione in cardioplegia and reperfusion.

Pflugers Arch 2019 Apr 6;471(4):583-593. Epub 2019 Jan 6.

Laboratory for Myocardial Metabolism, National Medical Research Center for Cardiology, 3rd Cherepkovskaya Str., 15A, Moscow, Russian Federation, 121552.

Disturbed homeostasis of nitric oxide (NO) is one of the causes of myocardial ischemia/reperfusion (I/R) injury during open-heart surgery. This study was designed to explore mechanisms of action of dinitrosyl iron complexes with reduced glutathione ({(GS)Fe(NO)}, DNIC-GS) added to crystalloid cardioplegia or reperfusion solution in isolated working rat hearts. Hearts of male Wistar rats were subjected to cardioplegic arrest by St. Read More

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http://dx.doi.org/10.1007/s00424-018-02251-2DOI Listing
April 2019
3 Reads

Four novel interaction partners demonstrate diverse modulatory effects on voltage-gated Ca2.2 Ca channels.

Pflugers Arch 2019 Jan 5. Epub 2019 Jan 5.

Center of Bioscience, Institute for Molecular Physiology and Genetics, 84005, Bratislava, Slovakia.

Voltage-gated Ca channels are embedded in a network of protein interactions that are fundamental for channel function and modulation. Different strategies such as high-resolution quantitative MS analyses and yeast-two hybrid screens have been used to uncover these Ca channel nanodomains. We applied the yeast split-ubiquitin system with its specific advantages to search for interaction partners of the Ca2. Read More

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http://dx.doi.org/10.1007/s00424-018-02248-xDOI Listing
January 2019
3 Reads
4.101 Impact Factor

Hibernating astronauts-science or fiction?

Pflugers Arch 2018 Dec 19. Epub 2018 Dec 19.

Animal Physiology, Faculty of Biology, Marburg University, Karl-von-Frisch-Straße 8, 35043, Marburg, Germany.

For long-duration manned space missions to Mars and beyond, reduction of astronaut metabolism by torpor, the metabolic state during hibernation of animals, would be a game changer: Water and food intake could be reduced by up to 75% and thus reducing payload of the spacecraft. Metabolic rate reduction in natural torpor is linked to profound changes in biochemical processes, i.e. Read More

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http://dx.doi.org/10.1007/s00424-018-2244-7DOI Listing
December 2018
1 Read

Phosphate transport: from microperfusion to molecular cloning.

Pflugers Arch 2019 Jan 19;471(1):1-6. Epub 2018 Dec 19.

Epithelial Research Group, Newcastle University, Institute for Cell and Molecular Biosciences, Newcastle, UK.

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http://dx.doi.org/10.1007/s00424-018-2245-6DOI Listing
January 2019
1 Read

Electrophysiology of the pancreatic islet β-cell sweet taste receptor TIR3.

Pflugers Arch 2019 Apr 14;471(4):647-654. Epub 2018 Dec 14.

Institute for Molecular and Cellular Regulation, Gunma University, Maebashi, Japan.

Over recent years, the presence of the sweet taste receptor TIR3 in rodent and human insulin-producing pancreatic islet β-cells was documented. The activation of this receptor by sweet-tasting sucralose mimics several biochemical and functional effects of D-glucose in the β-cells. The present study extends this analogy to the bioelectrical response of β-cells. Read More

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http://link.springer.com/10.1007/s00424-018-2237-6
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http://dx.doi.org/10.1007/s00424-018-2237-6DOI Listing
April 2019
10 Reads

Clinical aspects of the phosphate transporters NaPi-IIa and NaPi-IIb: mutations and disease associations.

Pflugers Arch 2019 Jan 13;471(1):137-148. Epub 2018 Dec 13.

Institute of Physiology, University of Zurich, Winterthurerstrasse 190, CH-8057, Zurich, Switzerland.

The Na-dependent phosphate transporter NaPi-IIa (SLC34A1) is mostly expressed in kidney, whereas NaPi-IIb (SLC34A2) has a wider tissue distribution with prominent expression in the lung and small intestine. NaPi-IIa is involved in renal reabsorption of inorganic phosphate (Pi) from urine, and patients with biallelic inactivating mutations in SLC34A1 develop hypophosphatemia, hypercalcemia, hypercalciuria and nephrocalcinosis, and nephrolithiasis in early childhood. Monoallelic mutations are frequent in the general population and may impact on the risk to develop kidney stones in adulthood. Read More

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http://dx.doi.org/10.1007/s00424-018-2246-5DOI Listing
January 2019
2 Reads

Type II Na-phosphate Cotransporters and Phosphate Balance in Teleost Fish.

Pflugers Arch 2019 Jan 12;471(1):193-212. Epub 2018 Dec 12.

Epithelial Research Group, Institute for Cell and Molecular Biosciences, Newcastle University, Newcastle upon Tyne, UK.

Teleost fish are excellent models to study the phylogeny of the slc34 gene family, Slc34-mediated phosphate (P) transport and how Slc34 transporters contribute P homeostasis. Fish need to accumulate P from the diet to sustain growth. Much alike in mammals, intestinal uptake in fish is partly a paracellular and partly a Slc34-mediated transcellular process. Read More

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http://dx.doi.org/10.1007/s00424-018-2239-4DOI Listing
January 2019
1 Read

Systemic network for dietary inorganic phosphate adaptation among three organs.

Pflugers Arch 2019 Jan 6;471(1):123-136. Epub 2018 Dec 6.

Department of Molecular Nutrition, Institute of Biomedical Sciences, University of Tokushima Graduate School, 3-18-15 Kuramoto-Cho, Tokushima City, 770-8503, Japan.

Inorganic phosphate (Pi) secretion from the salivary glands and dietary Pi are key Pi sources. The regulatory mechanisms of Pi homeostasis in the salivary glands are unknown. We investigated how salivary Pi concentrations are regulated by dietary Pi in mouse models. Read More

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http://dx.doi.org/10.1007/s00424-018-2242-9DOI Listing
January 2019
2 Reads

Substrates and inhibitors of phosphate transporters: from experimental tools to pathophysiological relevance.

Pflugers Arch 2019 Jan 7;471(1):53-65. Epub 2018 Dec 7.

Department of Toxicology, Veterinary Faculty, University of Zaragoza, Calle Miguel Servet, 177, E50013, Zaragoza, Spain.

The control of inorganic phosphate homeostasis is mediated through the activity of sodium-coupled Pi transporters located in the intestine, kidneys, and bone. To study these transporters in either the native tissue or after heterologous expression, it is very important to use specific inhibitors of the studied transporter, in order to know the corresponding relevance in the total Pi uptake and to differentiate from the activity of other transporters. Inhibitors are also necessary as drugs for treating Pi homeostasis disorders. Read More

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http://dx.doi.org/10.1007/s00424-018-2241-xDOI Listing
January 2019
4 Reads

Robustness of the non-neuronal cholinergic system in rat large intestine against luminal challenges.

Pflugers Arch 2019 Apr 1;471(4):605-618. Epub 2018 Dec 1.

Institute for Veterinary Physiology and Biochemistry, Justus Liebig University Giessen, Giessen, Germany.

Acetylcholine and atypical esters of choline such as propionyl- and butyrylcholine are produced by the colonic epithelium and are released when epithelial receptors for short-chain fatty acids (SCFA) are stimulated by propionate. It is assumed that the SCFA used by the choline acetyltransferase (ChAT), the central enzyme for the production of these choline esters, originate from the colonic lumen, where they are synthesized during the bacterial fermentation of carbohydrates. Therefore, it seemed to be of interest to study whether the non-neuronal cholinergic system in the colonic epithelium is affected by maneuvers intended to stimulate or to inhibit colonic fermentation by changing the intestinal microbiota. Read More

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http://dx.doi.org/10.1007/s00424-018-2236-7DOI Listing
April 2019
7 Reads
4.101 Impact Factor

Role of αKlotho and FGF23 in regulation of type II Na-dependent phosphate co-transporters.

Pflugers Arch 2019 Jan 1;471(1):99-108. Epub 2018 Dec 1.

Charles and Jane Pak Center for Mineral Metabolism and Clinical Research, University of Texas Southwestern Medical Center, 5323 Harry Hines Blvd., Dallas, TX, 75390, USA.

Alpha-Klotho is a member of the Klotho family consisting of two other single-pass transmembrane proteins: βKlotho and γKlotho; αKlotho has been shown to circulate in the blood. Fibroblast growth factor (FGF)23 is a member of the FGF superfamily of 22 genes/proteins. αKlotho serves as a co-receptor with FGF receptors (FGFRs) to provide a receptacle for physiological FGF23 signaling including regulation of phosphate metabolism. Read More

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http://dx.doi.org/10.1007/s00424-018-2238-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6324980PMC
January 2019
8 Reads

Expression and function of Slc34 sodium-phosphate co-transporters in skeleton and teeth.

Authors:
Laurent Beck

Pflugers Arch 2019 Jan 3;471(1):175-184. Epub 2018 Dec 3.

INSERM, UMR 1229, RMeS, Regenerative Medicine and Skeleton, Faculté de Chirurgie Dentaire, Université de Nantes, ONIRIS, 1 place Alexis Ricordeau, 44042, Nantes, France.

Under normal physiological condition, the biomineralization process is limited to skeletal tissues and teeth and occurs throughout the individual's life. Biomineralization is an actively regulated process involving the progressive mineralization of the extracellular matrix secreted by osteoblasts in bone or odontoblasts and ameloblasts in tooth. Although the detailed molecular mechanisms underlying the formation of calcium-phosphate apatite crystals are still debated, it is suggested that calcium and phosphate may need to be transported across the membrane of the mineralizing cell, suggesting a pivotal role of phosphate transporters in bone and tooth mineralization. Read More

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http://dx.doi.org/10.1007/s00424-018-2240-yDOI Listing
January 2019
1 Read

Allelic imbalance and haploinsufficiency in MYBPC3-linked hypertrophic cardiomyopathy.

Pflugers Arch 2018 Nov 20. Epub 2018 Nov 20.

Department of Molecular and Integrative Physiology, University of Michigan, 7220 MSRB III, 1150 W Medical Center Drive, SPC 0644, Ann Arbor, MI, 48109-5853, USA.

Mutations in cardiac myosin binding protein C (MYBPC3) represent the most frequent cause of familial hypertrophic cardiomyopathy (HCM), making up approximately 50% of identified HCM mutations. MYBPC3 is distinct among other sarcomere genes associated with HCM in that truncating mutations make up the vast majority, whereas nontruncating mutations predominant in other sarcomere genes. Several studies using myocardial tissue from HCM patients have found reduced abundance of wild-type MYBPC3 compared to control hearts, suggesting haploinsufficiency of full-length MYBPC3. Read More

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http://link.springer.com/10.1007/s00424-018-2226-9
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http://dx.doi.org/10.1007/s00424-018-2226-9DOI Listing
November 2018
14 Reads

Probiotics ameliorate renal ischemia-reperfusion injury by modulating the phenotype of macrophages through the IL-10/GSK-3β/PTEN signaling pathway.

Pflugers Arch 2019 Apr 13;471(4):573-581. Epub 2018 Nov 13.

Department of Kidney Transplantation, Nephropathy Hospital, the First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, 710061, China.

After renal ischemic reperfusion injury, a series of pathological changes, such as impaired intestinal barrier function, intestinal flora, and endotoxin translocation, are caused by intestinal ischemia and hypoxia, which then trigger systemic inflammatory responses and affect the condition and prognosis of the patients. In this study, a rat model of ischemia-reperfusion injury was established by examining changes in renal function, intestinal barrier function, inflammatory index, oxidative stress, and macrophage phenotypes to evaluate the effect of probiotic VSL#3 on renal ischemia-reperfusion injury. The results showed that, after VSL#3 intervention, the levels of BUN, Scr, Cys C, PRO, and NGAL were all significantly decreased compared with the I/R group, while the value of Ccr showed a significant increase. Read More

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http://link.springer.com/10.1007/s00424-018-2213-1
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http://dx.doi.org/10.1007/s00424-018-2213-1DOI Listing
April 2019
14 Reads
4.100 Impact Factor

Endurance exercise protects skeletal muscle against both doxorubicin-induced and inactivity-induced muscle wasting.

Pflugers Arch 2019 Mar 13;471(3):441-453. Epub 2018 Nov 13.

Department of Applied Physiology and Kinesiology, University of Florida, Gainesville, FL, 32611, USA.

Repeated bouts of endurance exercise promotes numerous biochemical adaptations in skeletal muscle fibers resulting in a muscle phenotype that is protected against a variety of homeostatic challenges; these exercise-induced changes in muscle phenotype are often referred to as "exercise preconditioning." Importantly, exercise preconditioning provides protection against several threats to skeletal muscle health including cancer chemotherapy (e.g. Read More

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http://link.springer.com/10.1007/s00424-018-2227-8
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http://dx.doi.org/10.1007/s00424-018-2227-8DOI Listing
March 2019
31 Reads

Impact of high- and low-intensity resistance training on arterial stiffness and blood pressure in adults across the lifespan: a review.

Pflugers Arch 2019 Mar 13;471(3):467-478. Epub 2018 Nov 13.

Department of Exercise Sciences, Lindenwood University, Belleville, IL, 62226, USA.

Resistance training (RT) is performed for improvements in body composition in young healthy adults and for health benefits in middle-aged and older adults. Traditionally, RT is prescribed at moderate- to high-intensity to promote benefits on skeletal muscle mass and strength in middle-aged and older adults without considering the vascular effects. Recent evidence suggests that muscle strength may be more protective than muscle mass for cardiovascular disease prevention and that muscle strength can be importantly improved with low-intensity RT. Read More

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http://dx.doi.org/10.1007/s00424-018-2235-8DOI Listing
March 2019
4 Reads

SLC41A1 is essential for magnesium homeostasis in vivo.

Pflugers Arch 2018 Nov 12. Epub 2018 Nov 12.

Department of Physiology, Radboud Institute for Molecular Life Sciences, Radboud University Medical Center, Nijmegen, The Netherlands.

Solute carrier family 41 member A1 (SLC41A1) has been suggested to mediate magnesium (Mg) transport by several in vitro studies. However, the physiological function of SLC41A1 remains to be elucidated. In this study, cellular Mg transport assays combined with zebrafish slc41a1 knockdown experiments were performed to disclose SLC41A1 function and its physiological relevance. Read More

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http://dx.doi.org/10.1007/s00424-018-2234-9DOI Listing
November 2018
2 Reads

Modulation of K channel N-type inactivation by sulfhydration through hydrogen sulfide and polysulfides.

Pflugers Arch 2019 Apr 10;471(4):557-571. Epub 2018 Nov 10.

Center for Molecular Biomedicine, Department of Biophysics, Friedrich Schiller University Jena and Jena University Hospital, Hans-Knöll-Str. 2, 07745, Jena, Germany.

Fast N-type inactivation of voltage-gated K (Kv) channels is important in fine-tuning of cellular excitability. To serve diverse cellular needs, N-type inactivation is regulated by numerous mechanisms. Here, we address how reactive sulfur species-the gaseous messenger HS and polysulfides-affect N-type inactivation of the mammalian Kv channels Kv1. Read More

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http://link.springer.com/10.1007/s00424-018-2233-x
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http://dx.doi.org/10.1007/s00424-018-2233-xDOI Listing
April 2019
15 Reads

Endothelial prostacyclin protects the kidney from ischemia-reperfusion injury.

Pflugers Arch 2019 Apr 9;471(4):543-555. Epub 2018 Nov 9.

Division of Nephrology, Huashan Hospital, Fudan University, 12 Wulumuqi Road (middle), Shanghai, 200040, China.

Prostacyclin, or PGI, is a product of PGI synthase (PGIS), down-stream of cyclooxygenase pathway. PGI has been demonstrated to play an important role in maintaining renal blood flow. Non-steroidal anti-inflammatory drugs (NSAIDs) that inhibit cyclooxygenase are reported to increase the susceptibility of patients to acute kidney injury (AKI). Read More

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http://link.springer.com/10.1007/s00424-018-2229-6
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http://dx.doi.org/10.1007/s00424-018-2229-6DOI Listing
April 2019
8 Reads

Inhibitory effect of luminal saccharides on glucose absorption from an adjacent jejunal site in rats: a newly described intestinal neural reflex.

Pflugers Arch 2019 Apr 6;471(4):595-603. Epub 2018 Nov 6.

Department of Anatomy, Cell Biology and Physiology, American University of Beirut, P.O. Box 113-6044, Beirut, Lebanon.

Nutrients in the lumen of the small intestine are sensed by special cells in the epithelial lining. The ensuing neurohumoral reflexes affect gastrointestinal absorption/secretion, motility, and vascular perfusion. To study in vivo the effect of a monosaccharide (glucose) or polysaccharide (starch) present in the jejunum on glucose absorption from an adjacent part of the intestine and investigate the possible underlying mechanisms. Read More

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http://link.springer.com/10.1007/s00424-018-2230-0
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http://dx.doi.org/10.1007/s00424-018-2230-0DOI Listing
April 2019
16 Reads

Aquaporins in the lung.

Pflugers Arch 2019 Apr 5;471(4):519-532. Epub 2018 Nov 5.

Institute of General Physiology, Ulm University, Albert-Einstein-Allee 11, 89081, Ulm, Germany.

The lung is the interface between air and blood where the exchange of oxygen and carbon dioxide occurs. The surface liquid that is directly exposed to the gaseous compartment covers both conducting airways and respiratory zone and forms the air-liquid interface. The barrier that separates this lining fluid of the airways and alveoli from the extracellular compartment is the pulmonary epithelium. Read More

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http://link.springer.com/10.1007/s00424-018-2232-y
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http://dx.doi.org/10.1007/s00424-018-2232-yDOI Listing
April 2019
16 Reads

Physiological regulation of phosphate by vitamin D, parathyroid hormone (PTH) and phosphate (Pi).

Pflugers Arch 2019 Jan 5;471(1):83-98. Epub 2018 Nov 5.

Centre for Nephrology, University College London (UCL), Royal Free Campus, Rowland Hill Street, London, NW3 2PF, UK.

Inorganic phosphate (Pi) is an abundant element in the body and is essential for a wide variety of key biological processes. It plays an essential role in cellular energy metabolism and cell signalling, e.g. Read More

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http://link.springer.com/10.1007/s00424-018-2231-z
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http://dx.doi.org/10.1007/s00424-018-2231-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6326012PMC
January 2019
26 Reads

Old Friends, immunoregulation, and stress resilience.

Pflugers Arch 2019 Feb 1;471(2):237-269. Epub 2018 Nov 1.

Laboratory for Molecular Psychosomatics, Department of Psychosomatic Medicine and Psychotherapy, University Ulm, Albert-Einstein-Allee 23, 89081, Ulm, Germany.

There is a considerable body of evidence indicating that chronic adverse experience, especially chronic psychosocial stress/trauma, represents a major risk factor for the development of many somatic and affective disorders, including inflammatory bowel disease (IBD) and posttraumatic stress disorder (PTSD). However, the mechanisms underlying the development of chronic stress-associated disorders are still in large part unknown, and current treatment and prevention strategies lack efficacy and reliability. A greater understanding of mechanisms involved in the development and persistence of chronic stress-induced disorders may lead to novel approaches to prevention and treatment of these disorders. Read More

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http://link.springer.com/10.1007/s00424-018-2228-7
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http://dx.doi.org/10.1007/s00424-018-2228-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6334733PMC
February 2019
6 Reads

Coronary arterial vasculature in the pathophysiology of hypertrophic cardiomyopathy.

Pflugers Arch 2018 Oct 29. Epub 2018 Oct 29.

Department of Physiology and Biophysics and the Center for Cardiovascular Research, University of Illinois at Chicago, 835 S Wolcott Ave, Chicago, IL, 60612, USA.

Alterations in the coronary vascular system are likely associated with a mismatch between energy demand and energy supply and critical in triggering the cascade of events that leads to symptomatic hypertrophic cardiomyopathy. Targeting the early events, particularly vascular remodeling, may be a key approach to developing effective treatments. Improvement in our understanding of hypertrophic cardiomyopathy began with the results of early biophysical studies, proceeded to genetic analyses pinpointing the mutational origin, and now pertains to imaging of the metabolic and flow-related consequences of such mutations. Read More

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http://link.springer.com/10.1007/s00424-018-2224-y
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http://dx.doi.org/10.1007/s00424-018-2224-yDOI Listing
October 2018
23 Reads

Regulation of autophagic and mitophagic flux during chronic contractile activity-induced muscle adaptations.

Pflugers Arch 2019 Mar 27;471(3):431-440. Epub 2018 Oct 27.

Muscle Health Research Centre, Toronto, ON, Canada.

Autophagy and mitophagy are important for training-inducible muscle adaptations, yet it remains unclear how these systems are regulated throughout the adaptation process. Here, we studied autophagic and mitophagic flux in the skeletal muscles of Sprague-Dawley rats (300-500 g) exposed to chronic contractile activity (CCA; 3 h/day, 9 V, 10 Hz continuous, 0.1 ms pulse duration) for 1, 2, 5, and 7 days (N = 6-8/group). Read More

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http://link.springer.com/10.1007/s00424-018-2225-x
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http://dx.doi.org/10.1007/s00424-018-2225-xDOI Listing
March 2019
7 Reads