2,628,050 results match your criteria Peptides [Journal]


An affinity peptide exerts antiviral activity by strongly binding nervous necrosis virus to block viral entry.

Fish Shellfish Immunol 2018 Dec 3. Epub 2018 Dec 3.

State Key Laboratory of Biocontrol, Institute of Aquatic Economic Animals and Guangdong Provincial Key Laboratory of Improved Variety Reproduction in Aquatic Economic Animals, School of Life Sciences, Sun Yat-sen University, Guangzhou, China. Electronic address:

Nervous necrosis virus (NNV) causes viral nervous necrosis (VNN), a disease that leads to almost 100% mortality among larvae and juvenile fish, severely affecting the aquaculture industry. VNN vaccines based on inactivated viruses or virus-like particles (VLPs) are unsuitable for fish fry with immature adaptive immune systems. Here, we applied an anti-NNV strategy based on affinity peptides (AFPs). Read More

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December 2018
1 Read

The novel cationic cell-penetrating peptide PEP-NJSM is highly active against Staphylococcus epidermidis biofilm.

Int J Biol Macromol 2018 Dec 3. Epub 2018 Dec 3.

Laboratory of Molecular and Cellular Screening Processes, Centre of Biotechnology of Sfax, University of Sfax, Road Sidi Mansour km 6, PO BOX 1177, 3018 Sfax, Tunisia.

A cationic cell-penetrating peptide PEP-NJSM was identified in human virus proteomes by a screening of charge clusters in protein sequences generating cell penetrating peptides (CPP). PEP-NJSM was selectively active against Gram positive Staphylococcus epidermidis as antibacterial agent with MIC value of 128 μM compared to the Gram-negative Pseudomonas aeruginosa strain with MIC value exceeded 512 μM. The selected peptide exhibited an important anti-biofilm activity even at sub-MIC levels. Read More

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December 2018
1 Read

Bioactivity of soy-based fermented foods: A review.

Biotechnol Adv 2018 Dec 3. Epub 2018 Dec 3.

College of Food Science and Technology, Yunnan Agricultural University, Kunming 650201, China. Electronic address:

For centuries, fermented soy foods have been dietary staples in Asia and, now, in response to consumer demand, they are available throughout the world. Fermentation bestows unique flavors, boosts nutritional values and increases or adds new functional properties. In this review, we describe the functional properties and underlying action mechanisms of soy-based fermented foods such as Natto, fermented soy milk, Tempeh and soy sauce. Read More

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December 2018
1 Read

A Radical Approach to Enzymatic β-Thioether Bond Formation.

J Am Chem Soc 2018 Dec 6. Epub 2018 Dec 6.

Ribosomally-synthesized and post-translationally-modified peptides (RiPPs) are an emerging class of natural products that harbor diverse chemical functionalities, usually introduced via the action of a small number of tailoring enzymes. We have been interested in RiPP biosynthetic gene clusters that encode unusual metalloenzymes, as these may install yet unknown alterations. Using a new bioinformatic search strategy, we recently identified an array of unexplored RiPP gene clusters that are quorum sensing-regulated and contain one or more uncharacterized radical S-adenosylmethionine (RaS) metalloenzymes. Read More

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December 2018
1 Read

Appearance of di- and Tripeptides in Human Plasma after a Protein Meal does not Correlate with PEPT1 Substrate Selectivity.

Mol Nutr Food Res 2018 Dec 6:e1801094. Epub 2018 Dec 6.

F. Rohm, Dr. H. Daniel, Dr. B. Spanier, Nutritional Physiology, Technical University of Munich, Freising, Germany.

Scope: PEPT1 function is well understood, yet little is known about its contribution towards the absorption of dietary amino acids in the form of di- and tripeptides. In the present human study, we investigated changes in plasma concentrations of a representative oligopeptide panel after meat intake.

Methods And Results: Based on an LC-MS/MS method for quantitative analysis of a panel of selected di- and tripeptides in biological samples, we describe the kinetics of plasma changes of peptides derived from a widely accessible dietary protein source. Read More

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December 2018

Isolation and identification of endogenous RFRP1 and RFRP3 in the mouse hypothalamus.

J Neuroendocrinol 2018 Dec 6:e12668. Epub 2018 Dec 6.

Department of Biological Functions of Food, Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, Olsztyn, Poland.

Although RFamide-related peptide (RFRP) preproprotein sequence is known in mice, until now the molecular structure of the mature, functional peptides processed from the target precursor molecule has not been determined. In the present study we purified endogenous RFRP1 and RFRP3 peptides from mouse hypothalamic tissue extracts using immunoaffinity column conjugated with specific antibodies against the mouse C-terminus of RFRP-1 and RFRP-3. Employing liquid chromatography coupled with mass spectrometry (LC-MS) we demonstrated, that RFRP1 consists of 15 and RFRP3 of 10 amino acid residues (ANKVPHSAANLPLRF-NH and SHFPSLPQRF-NH , respectively). Read More

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December 2018
1 Read

Introduction of D-Amino Acids in Minimalistic Peptide Substrates by an S-Adenosyl-L-Methionine Radical Epimerase.

Angew Chem Int Ed Engl 2018 Dec 6. Epub 2018 Dec 6.

ETH Zürich, Department of Biology, Vladimir-Prelog-Weg 4, 8093, Zürich, SWITZERLAND.

Post-translational modifying enzymes from the S-adenosyl-L-methionine (AdoMet) radical superfamily garner attention due to their ability to accomplish challenging biochemical reactions. Among them, a family of AdoMet radical epimerases catalyze irreversible L- to D-amino acid transformations of diverse residues, including 18 sites in the complex sponge-derived polytheonamide toxins. These enzymes are from the proteusin family of ribosomally synthesized and post-translationally modified peptide natural products and act on precursor proteins with relatively large N-terminal leaders and highly variable core sequences. Read More

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December 2018
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Annotation of the Giardia proteome through structure-based homology and machine learning.

Gigascience 2018 Dec 6. Epub 2018 Dec 6.

Walter & Eliza Hall Institute of Medical Research, Melbourne, Australia.

Background: Large-scale computational prediction of protein structures represents a cost-effective alternative to empirical structure determination with particular promise for non-model organisms and neglected pathogens. Conventional sequence-based tools are insufficient to annotate the genomes of such divergent biological systems. Conversely, protein structure tolerates substantial variation in primary amino acid sequence, and is thus a robust indicator of biochemical function. Read More

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December 2018
2 Reads

Fluorogenic probes for disease-relevant enzymes.

Chem Soc Rev 2018 Dec 6. Epub 2018 Dec 6.

Key Laboratory for Advanced Materials and Joint International Research Laboratory of Precision Chemistry and Molecular Engineering, Feringa Nobel Prize Scientist Joint Research Center, School of Chemistry and Molecular Engineering, East China University of Science and Technology, 130 Meilong Rd., Shanghai 200237, P. R. China.

Traditional biochemical methods for enzyme detection are mainly based on antibody-based immunoassays, which lack the ability to monitor the spatiotemporal distribution and, in particular, the in situ activity of enzymes in live cells and in vivo. In this review, we comprehensively summarize recent progress that has been made in the development of small-molecule as well as material-based fluorogenic probes for sensitive detection of the activities of enzymes that are related to a number of human diseases. The principles utilized to design these probes as well as their applications are reviewed. Read More

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December 2018

SecScan: a general approach for mapping disulfide bonds in synthetic and recombinant peptides and proteins.

Chem Commun (Camb) 2018 Dec 6. Epub 2018 Dec 6.

Department of Biochemistry, University of Maastricht, Cardiovascular Research Institute Maastricht (CARIM), Universiteitssingel 50, 6229 ER, Maastricht, The Netherlands.

Selenocysteine scanning (SecScan) is a novel technique to map disulfide networks in proteins independent of structure-based distance information and mass spectrometry. SecScan applies systematic substitution of single Cys by Sec in combination with NMR spectroscopy for reliable and unambiguous determination of disulfide bond networks. Read More

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December 2018
1 Read

Effects of sacubitril/valsartan on neprilysin targets and the metabolism of natriuretic peptides in chronic heart failure: a mechanistic clinical study.

Eur J Heart Fail 2018 Dec 6. Epub 2018 Dec 6.

Inserm UMR-S 942, Lariboisière Hospital, Paris, France.

Aim: This study aimed at evaluating the effects of sacubitril/valsartan on neprilysin (NEP), and the metabolism of natriuretic peptides in heart failure (HF) and providing additional mechanistic information on the mode of action of the drug.

Methods And Results: We enrolled 73 chronic HF patients who were switched from angiotensin-converting enzyme inhibitor or angiotensin receptor blocker to sacubitril/valsartan. In addition to clinical and echocardiographic assessment, plasma biomarkers were measured at baseline, day 30 and day 90 after initiation of treatment. Read More

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December 2018

M918: A novel cell penetrating peptide for effective delivery of HIV-1 Nef and Hsp20-Nef proteins into eukaryotic cell lines.

Curr HIV Res 2018 Dec 5. Epub 2018 Dec 5.

Pilot Nano-Biotechnology Department, Pasteur Institute of Iran, Tehran. Iran.

Background: HIV-1 Nef protein is a possible attractive target in development of therapeutic HIV vaccines including protein-based vaccines. The most important disadvantage of protein-based vaccines is their low immunogenicity which can be improved by heat shock proteins (Hsps) as an immunomodulator, and cell penetrating peptides (CPPs) as a carrier.

Methods: In this study, the HIV-1 Nef and Hsp20-Nef proteins were generated in E. Read More

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December 2018
3 Reads

A computational and experimental study to develop E-selectin targeted peptides for molecular imaging applications.

Future Med Chem 2018 Dec 6. Epub 2018 Dec 6.

Aix Marseille Univ, CNRS, Cent Marseille, ISM2, Marseille, France.

Aim: E-selectin is overexpressed on angiogenic and inflamed endothelium. Molecules binding to E-selectin with high affinity and specificity enable its use as a molecular imaging biomarker.

Material & Methods: The interactions of four different peptides (i. Read More

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December 2018

Rapid and Accurate Structure-Based Therapeutic Peptide Design using GPU Accelerated Thermodynamic Integration.

Proteins 2018 Dec 6. Epub 2018 Dec 6.

Donnelly Centre for Cellular and Biomolecular Research, University of Toronto, Toronto M5S 3E1, Canada.

Peptide-based therapeutics are an alternative to small molecule drugs as they offer superior specificity, lower toxicity and easy synthesis. Here we present an approach that leverages the dramatic performance increase afforded by the recent arrival of GPU accelerated thermodynamic integration (TI). GPU TI facilitates very fast, highly accurate binding affinity optimization of peptides against therapeutic targets. Read More

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December 2018

Two ancient neuropeptides, PACAP and AVP, modulate motivated behavior at synapses in the extrahypothalamic brain: a study in contrast.

Cell Tissue Res 2018 Dec 5. Epub 2018 Dec 5.

Section on Molecular Neuroscience, Laboratory of Cellular and Molecular Regulation, NIMH-IRP, Building 49, Room 5A-32, 9000 Rockville Pike, Bethesda, MD, USA.

We examine evolutionary aspects of two primordial neuropeptides, arginine vasopressin (AVP) and pituitary adenylate cyclase-activating polypeptide (PACAP); the distribution of AVP and PACAP and their receptors in mammals; AVP and PACAP release patterns relevant to their roles in neuroendocrine control in brain and periphery; and finally the intricate interlocking of homeostatic and allostatic regulation created by extrahypothalamic AVP and PACAP projections to brain circuit nodes important in controlling appetitive, avoidance and aggressive motor responses. A cardinal feature of peptide neurotransmission important in regulatory control of organismic responses and emphasized in this review, is that neuropeptides are released from large dense-core vesicles docked not only within axonal varicosities and dendrites but also at presynaptic nerve terminal sites, along with small clear synaptic vesicles, at active zones. Peptide transmitter nerve terminals, from hypothalamic and other projections, are distributed widely to multiple brain areas important in integrative control of behavior. Read More

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December 2018

Involvement of natriuretic peptide system in C2C12 myocytes.

Mol Cell Biochem 2018 Dec 5. Epub 2018 Dec 5.

Sohyaku Innovative Research Division, Mitsubishi Tanabe Pharma Corporation, 2-2-50, Kawagishi, Toda, Saitama, 335-8505, Japan.

The natriuretic peptide system, a key regulator of cGMP signaling, comprises three types of natriuretic peptides, osteocrin/musclin (OSTN), and their natriuretic peptide receptors. Although this system plays important roles in many organs, its physiological roles in skeletal muscle have not been clearly described. In the present study, we investigated the role of the natriuretic peptide system in C2C12 myocytes. Read More

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December 2018
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Polypeptide-affined interpenetrating hydrogels with tunable physical and mechanical properties.

Biomater Sci 2018 Dec 6. Epub 2018 Dec 6.

School of Chemical and Biomolecular Engineering, The University of Sydney, Sydney, NSW 2006, Australia.

Novel hydrogels with tunable mechanical properties similar to human soft tissue have increasing applications in biomedicine, soft robotics, and biocompatible electronics. However, most of these materials require multiple-step fabrication, are not robust, and compromise bioactivity. Thus, aiming to address these shortfalls, herein, we report a versatile hydrogel system with tunable properties and a facile one-pot fabrication process. Read More

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December 2018
4 Reads

Celiac Disease: A Review of Current Concepts in Pathogenesis, Prevention, and Novel Therapies.

Front Pediatr 2018 21;6:350. Epub 2018 Nov 21.

The Diabetes and Celiac Disease Unit, Department of Clinical Sciences, Lund University, Malmö, Sweden.

Our understanding of celiac disease and how it develops has evolved significantly over the last half century. Although traditionally viewed as a pediatric illness characterized by malabsorption, it is now better seen as an immune illness with systemic manifestations affecting all ages. Population studies reveal this global disease is common and, in many countries, increasing in prevalence. Read More

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November 2018
6 Reads

Needle lost in the haystack: multiple reaction monitoring fails to detect candidate protein biomarkers in plasma and urine samples from individuals with syphilis.

F1000Res 2018 19;7:336. Epub 2018 Mar 19.

HIV/STI Unit, Institute of Tropical Medicine, Antwerp, 2000, Belgium.

Current syphilis diagnostic strategies are lacking a sensitive manner of directly detecting antigens. A diagnostic test that could directly detect antigens in individuals with syphilis would be of considerable clinical utility, especially for the diagnosis of reinfections and for post-treatment serological follow-up. In this study, 11 candidate biomarker proteins were chosen according to their physiochemical characteristics, specificity and predicted abundance. Read More

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March 2018
1 Read

Deep sequencing analysis of toad skin glands and partial biochemical characterization of its cutaneous secretion.

J Venom Anim Toxins Incl Trop Dis 2018 29;24:36. Epub 2018 Nov 29.

2Laboratory of genetics - LABGEN, Institute of Genetics and Biochemistry, Campus Umuarama, Federal University of Uberlândia, Avenida Pará, Uberlândia, MG 1720 Brazil.

Background: Animal poisons and venoms are sources of biomolecules naturally selected. toads are widespread in the whole Brazilian territory and they have poison glands and mucous gland. Recently, protein from toads' secretion has gaining attention. Read More

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November 2018
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Identification of a HIV Gp41-Specific Human Monoclonal Antibody With Potent Antibody-Dependent Cellular Cytotoxicity.

Front Immunol 2018 16;9:2613. Epub 2018 Nov 16.

AIDS Institute, Department of Microbiology, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong, Hong Kong.

Antibody-Dependent Cellular Cytotoxicity (ADCC) is a major mechanism of protection against viral infections . Identification of HIV-1-specific monoclonal antibodies (mAbs) with potent ADCC activity may help develop an effective HIV-1 vaccine. In present study, we isolated such human mAb, designated E10, from an HIV-1-infected patient sample by single B cell sorting and single cell PCR. Read More

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November 2018

The plasma peptidome.

Clin Proteomics 2018 1;15:39. Epub 2018 Dec 1.

1Ryerson Analytical Biochemistry Laboratory (RABL), Department of Chemistry and Biology, Faculty of Science, Ryerson University, 350 Victoria St, Toronto, ON Canada.

Background: It may be possible to discover new diagnostic or therapeutic peptides or proteins from blood plasma using LC-ESI-MS/MS to identify, with a linear quadrupole ion trap to identify, quantify and compare the statistical distributions of peptides cleaved ex vivo from plasma samples from different clinical populations.

Methods: A systematic method for the organic fractionation of plasma peptides was applied to identify and quantify the endogenous tryptic peptides from human plasma from multiple institutions by C18 HPLC followed nano electrospray ionization and tandem mass spectrometry (LC-ESI-MS/MS) with a linear quadrupole ion trap. The endogenous tryptic peptides, or tryptic phospho peptides (i. Read More

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December 2018

Mechanisms of deformable nanovesicles based on insulin-phospholipid complex for enhancing buccal delivery of insulin.

Int J Nanomedicine 2018 9;13:7319-7331. Epub 2018 Nov 9.

State Key Laboratory of Bioactive Substance and Function of Natural Medicines, Institute of Materia Medica, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100050, China,

Background: Non-injectable delivery of peptides and proteins are not feasible due to its large molecular, high hydrophilic and gastrointestinal degradation. Therefore, proposing a new method to solve this problem is a burning issue.

Purpose: The objective of this study was to propose a novel protein delivery strategy to vanquish the poor efficacy of buccal mucosa delivery systems for protein delivery and then investigate the detailed mechanisms of the enhanced buccal delivery of protein, using insulin as a model drug. Read More

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November 2018

A study on screening and antitumor effect of CD55-specific ligand peptide in cervical cancer cells.

Drug Des Devel Ther 2018 13;12:3899-3912. Epub 2018 Nov 13.

Department of Genetics and Cell Biology, Basic Medical College, Qingdao University, Qingdao 266071, People's Republic of China,

Background: To improve the targeting ability of antitumor drugs, we identified the antigens with high expression on the surface of tumor cells associated with tumor escape, such as the complement regulatory protein CD55 molecule, which is also known as the decay accelerating factor. In this study, phage display technology was used to screen and identify CD55-specific ligand peptide (CD55sp) bound to CD55 molecule on the surface of cervical cancer HeLa cells. We then explored the role of this peptide in inhibiting the growth of cervical cancer cells in vitro. Read More

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November 2018

Effective nose-to-brain delivery of exendin-4 via coadministration with cell-penetrating peptides for improving progressive cognitive dysfunction.

Sci Rep 2018 Dec 5;8(1):17641. Epub 2018 Dec 5.

Laboratory of Drug Delivery Systems, Faculty of Pharmaceutical Sciences, Kobe Gakuin University, 1-1-3 Minatojima, Chuo-ku, Kobe, Hyogo, 650-8586, Japan.

In a recent study, we demonstrated the potential of a cell-penetrating peptide (CPP) penetratin to deliver the peptide drug insulin to the brain via nasal administration, and its pharmacological effect on the mild cognitive dysfunction in senescence-accelerated mouse (SAMP8). However, the therapeutic potential of intranasal insulin administration was attenuated when applied to the aged SAMP8 with severe cognitive dysfunction. The present study, therefore, aimed to overcome the difficulty in treating severe cognitive dysfunction using insulin by investigating potential alternatives, glucagon-like peptide-1 (GLP-1) receptor agonists such as exendin-4. Read More

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December 2018

Endogenous toxins and the coupling of gregariousness to conspicuousness in Argidae and Pergidae sawflies.

Sci Rep 2018 Dec 5;8(1):17636. Epub 2018 Dec 5.

SNSB - Zoologische Staatssammlung München, Münchhausenstr. 21, 81247, Munich, Germany.

Phytophagous insects tend to be either cryptic and solitary, or brightly colored and gregarious, as a defense against vertebrate predators. Here, we tested whether potent defensive chemicals produced de novo by larvae of Argidae and Pergidae sawflies have influenced the evolutionary relationship between larval appearance and levels of gregariousness. Phylogeny-based correlation analyses indicated only a weak trend for solitary species to be cryptic, and for gregarious ones to be conspicuous. Read More

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December 2018

Novel and conventional approaches for the analysis of quantitative proteomic data are complementary towards the identification of seminal plasma alterations in infertile patients.

Mol Cell Proteomics 2018 Dec 5. Epub 2018 Dec 5.

Molecular Biology of Reproduction and Development, Human Genetics Unit, Faculty of Medicine, August Pi i Sunyer Biomedical Research Institute (IDIBAPS), University of Barcelona, and Hospital Clinic, Spain

Our aim was to define seminal plasma proteome signatures of infertile patients categorized according to their seminal parameters using TMT-LC-MS/MS. To that extent, quantitative proteomic data was analyzed following two complementary strategies: (i) the conventional approach based on standard statistical analyses of relative protein quantification values; and (ii) a novel strategy focused on establishing stable-protein pairs. By conventional analyses, the abundance of some seminal plasma proteins was found to be positively correlated with sperm concentration. Read More

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December 2018

Investigating the RAS can be a fishy business: interdisciplinary opportunities using Zebrafish.

Clin Sci (Lond) 2018 Dec 5;132(23):2469-2481. Epub 2018 Dec 5.

University of Edinburgh/BHF Centre for Cardiovascular Science, Queen's Medical Research Institute, The University of Edinburgh, 47, Little France Crescent, Edinburgh EH16 4TJ, U.K.

The renin-angiotensin system (RAS) is highly conserved, and components of the RAS are present in all vertebrates to some degree. Although the RAS has been studied since the discovery of renin, its biological role continues to broaden with the identification and characterization of new peptides. The evolutionarily distant zebrafish is a remarkable model for studying the kidney due to its genetic tractability and accessibility for imaging. Read More

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December 2018

Validation of extracellular ligand-receptor interactions by Flow-TriCEPS.

BMC Res Notes 2018 Dec 5;11(1):863. Epub 2018 Dec 5.

Dualsystems Biotech A.G., Grabenstrasse 11a, 8952, Schlieren, Switzerland.

Objective: The advent of ligand-based receptor capture methodologies, allows the identification of unknown receptor candidates for orphan extracellular ligands. However, further target validation can be tedious, laborious and time-consuming. Here, we present a methodology that provides a fast and cost-efficient alternative for candidate target verification on living cells. Read More

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December 2018

Evaluation of ERAP1 Gene Single Nucleotide Polymorphism in Impressing the Inflammatory Cytokine Profile of Ankylosing Spondylitis Patients.

Iran J Allergy Asthma Immunol 2018 Oct 20;17(5):464-474. Epub 2018 Oct 20.

Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran AND Department of Immunology, School of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran AND Student Research Committee, Tabriz University of Medical Sciences, Tabriz, Iran.

Ankylosing spondylitis (AS), an autoinflammatory disease, has been associated with impaired Endoplasmic reticulum aminopeptidase (ERAP) 1 activity, which is involved in priming antigenic peptides. The purpose of this study was to evaluate if the genetic variant of ERAP1 gene could impress the inflammation status of the AS patients. For genotyping, 140 AS cases and 140 healthy controls were enrolled. Read More

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October 2018

Assessment of the Inhibitory Effects of Ficin-hydrolyzed Gelatin Derived from Squid (Uroteuthis duvauceli) on Breast Cancer Cell Lines and Animal Model.

Iran J Allergy Asthma Immunol 2018 Oct 14;17(5):436-452. Epub 2018 Oct 14.

Department of Marine Science and Technology, Science and Research Branch, Islamic Azad University, Tehran, Iran.

Marine novel natural products have been applied for cancer therapy. Enzyme-digested gelatin hydrolysates have proven to serve as promising sources of potent biologically active peptides. Potential anti-breast cancer properties of the extracted Ficin-digesterd gelatin hydrolysate from Indian squid (Uroteuthis duvauceli) was extensively characterized by cellular and animal models. Read More

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October 2018

Design of Linear and Cyclic Mutant Analogues of Dirucotide Peptide (MBP) against Multiple Sclerosis: Conformational and Binding Studies to MHC Class II.

Brain Sci 2018 Dec 4;8(12). Epub 2018 Dec 4.

Department of Chemistry, University of Patras, 26500 Patras, Greece.

Background: Multiple sclerosis (MS) is an autoimmune disorder of the central nervous system. MS is a T cell-mediated disease characterized by the proliferation, infiltration, and attack of the myelin sheath by immune cells. Previous studies have shown that cyclization provides molecules with strict conformation that could modulate the immune system. Read More

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December 2018

A Systematic Overview of Type II and III Toxin-Antitoxin Systems with a Focus on Druggability.

Toxins (Basel) 2018 Dec 4;10(12). Epub 2018 Dec 4.

The Research Institute of Pharmaceutical Sciences, College of Pharmacy, Seoul National University, Gwanak-gu, Seoul 08826, Korea.

Toxin-antitoxin (TA) systems are known to play various roles in physiological processes, such as gene regulation, growth arrest and survival, in bacteria exposed to environmental stress. Type II TA systems comprise natural complexes consisting of protein toxins and antitoxins. Each toxin and antitoxin participates in distinct regulatory mechanisms depending on the type of TA system. Read More

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December 2018

TDP-43 specific reduction induced by Di-hydrophobic tags conjugated peptides.

Bioorg Chem 2018 Nov 27;84:254-259. Epub 2018 Nov 27.

Key Laboratory of Bioorganic Phosphorus Chemistry and Chemical Biology (Ministry of Education), Department of Chemistry, Tsinghua University, Beijing 100084, China; Beijing Institute for Brain Disorders, Beijing 100069, China; Center for Synthetic and Systems Biology, Tsinghua University, Beijing 100084, China. Electronic address:

TAR DNA binding protein 43 (TDP-43) is a key target in amyotrophic lateral sclerosis (ALS) treatment. Here, based on hydrophobic tagging strategy, we designed and synthesized a series of single or double hydrophobic tags conjugated peptides D1-D8. Among them, it was found that D4 displayed strongest ability to induce TDP-43 degradation in cells. Read More

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November 2018
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In vitro effect of oxidized and reduced glutathione peptides on angiotensin converting enzyme purified from human plasma.

J Chromatogr B Analyt Technol Biomed Life Sci 2018 Nov 19;1104:190-195. Epub 2018 Nov 19.

Van YüzüncüYıl University, Faculty of Science, Department of Chemistry, Van, Turkey.

Angiotensin converting enzyme (ACE, peptidyldipeptidase A, EC 3.4.15. Read More

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November 2018

Functionalization of hyaluronic acid hydrogels with ECM-derived peptides to control myoblast behavior.

Acta Biomater 2018 Nov 30. Epub 2018 Nov 30.

Weldon School of Biomedical Engineering, Purdue University, 206 S. Martin Jischke Dr., West Lafayette, IN, 47907, U.S.A. Electronic address:

Volumetric muscle loss (VML) occurs when skeletal muscle injury is too large for the body to fully self-repair. Typically, fibrotic tissue fills the void, which reduces muscle functionality and limb movement. Although a wide variety of natural and synthetic scaffolds have been studied with the purpose of providing the appropriate structural support, to date no scaffold has significantly restored muscle functionality after VML. Read More

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November 2018
1 Read

Identification of Expression and Function of the Glucagon-Like Peptide-1 Receptor in Colonic Smooth Muscle.

Peptides 2018 Nov 30. Epub 2018 Nov 30.

Department of Physiology and Biophysics, VCU Program in Enteric Neuromuscular Sciences, Virginia Commonwealth University, United States. Electronic address:

The insulinotropic effects of the incretin hormone, glucagon-like peptide-1 (GLP-1) are mediated via GLP-1 receptors (GLP-1R) present on pancreatic β cells. GLP-1 causes a decrease in the motility of stomach and intestine which involves both central and peripheral nervous systems. The expression and function of GLP-1R in gastrointestinal smooth muscle, however, are not clear. Read More

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November 2018

High doses of ANP and BNP exacerbate lipolysis in humans and the lipolytic effect of BNP is associated with cardiac triglyceride content in pigs.

Peptides 2018 Nov 30. Epub 2018 Nov 30.

Department of Clinical Biochemistry Copenhagen University Hospital, Rigshospitalet, Copenhagen, Denmark; Department of Clinical Medicine, Aarhus University Hospital, Aarhus, Denmark; Department of Cardiorenal physiology (Mayo Clinic, Rochester, Minnesota, USA; Copenhagen University, Denmark.

Drugs facilitating the cardioprotective effects of natriuretic peptides are introduced in heart failure treatment. ANP and BNP also stimulate lipolysis and increase circulating concentrations of free fatty acids (FFAs); an aspect, however, thought to be confined to primates. We examined the lipolytic effect of natriuretic peptide infusion in healthy young men and evaluated the effect in a porcine model of myocardial ischemia and reperfusion. Read More

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November 2018

Antimicrobial peptide KSL-W and analogues: Promising agents to control plant diseases.

Peptides 2018 Nov 30. Epub 2018 Nov 30.

LIPPSO, Department of Chemistry, University of Girona, Campus Montilivi, Girona, Spain. Electronic address:

Recent strong restrictions on the use of pesticides has prompted the search for safer alternatives, being antimicrobial peptides promising candidates. Herein, with the aim of identifying new agents, 15 peptides reported as plant defense elicitors, promiscuous, multifunctional or antimicrobial were selected and tested against six plant pathogenic bacteria of economic importance. Within this set, KSL-W (KKVVFWVKFK-NH) displayed high antibacterial activity against all the tested pathogens, low hemolysis and low phytotoxicity in tobacco leaves. Read More

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November 2018
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Antibacterial and anti-biofilm activity, and mechanism of action of pleurocidin against drug resistant Staphylococcus aureus.

Microb Pathog 2018 Nov 30. Epub 2018 Nov 30.

Department of Biomedical Science, Chosun University, Gwangju, 61452, South Korea; Research Center for Proteineous Materials, Chosun University, Gwangju, 61452, South Korea. Electronic address:

The abuse of antibiotics has resulted in the emergence of multi-drug-resistant bacteria. Staphylococcus aureus is a frequent cause of infections, and antibiotic-resistant S. aureus has become a serious problem. Read More

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November 2018

IN VITRO ANALYSIS OF SYNTHETIC PEPTIDES IN BLOCKING THE ENTRY OF DENGUE VIRUS.

Virus Res 2018 Nov 30. Epub 2018 Nov 30.

Department of Microbiology and Immunology, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand. Electronic address:

Dengue fever is the most prevalent arthropod-borne viral disease, and no specific therapeutic or promising antiviral drug is available for its treatment. Peptide inhibitors are less toxic than synthetic compounds and have found proven effective against viral infections. Here, three peptides that mimic part of the E protein of the dengue virus (DENV) were synthesized and evaluated for their inhibitory activity against four serotypes of DENV in African green monkey kidney (Vero) and rhesus macaque (Macaca mulatta) monkey kidney (LLC-MK2) cell lines. Read More

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November 2018

Sacubitril/valsartan (LCZ696) significantly reduces aldosterone and increases cGMP circulating levels in a canine model of RAAS activation.

Eur J Pharm Sci 2018 Nov 30. Epub 2018 Nov 30.

Novartis Institutes for BioMedical Research, East Hanover, NJ, USA.

Simultaneous blockade of angiotensin receptors and enhancement of natriuretic peptides (NP) by the first-in-class angiotensin receptor neprilysin (NEP) inhibitor sacubitril/valsartan constitutes an effective approach to treating heart failure. This study examined the effects of sacubitril/valsartan (225 and 675 mg/day) vs. placebo, sacubitril (360 mg/day), valsartan (900 mg/day), and benazepril (5 mg/day) on the dynamics of the renin-angiotensin-aldosterone system (RAAS) and the NP system in dogs. Read More

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November 2018

Ghrelin serves as a signal of energy utilization and is involved in maintaining energy homeostasis in broilers.

Gen Comp Endocrinol 2018 Nov 30. Epub 2018 Nov 30.

College of Animal Science and Technology, Shandong Key Laboratory of Animal Biotechnology and Disease Control and Prevention, Shandong Agricultural University, No. 61 Daizong Street, Taian, 271018, PR China. Electronic address:

Ghrelin, one of the most important appetite regulating peptides, is involved in the regulation of energy homeostasis. The anorexia effect of ghrelin in chickens is contrary to that of ghrelin in mammals. In the present study, the effects of feeding status and dietary energy level on plasma total ghrelin levels and expression were studied in broilers. Read More

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November 2018

Editorial overview: gastrointestinal regulatory peptides.

Curr Opin Endocrinol Diabetes Obes 2018 Nov 30. Epub 2018 Nov 30.

Boston University School of Medicine, Section of Gastroenterology, Boston, Massachusetts, USA.

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November 2018

Crystal structure analysis and enzymatic characterization of γ-glutamyltranspeptidase from Pseudomonas nitroreducens.

Biosci Biotechnol Biochem 2018 Dec 3:1-8. Epub 2018 Dec 3.

b Department of Biotechnology , College of Life Sciences, Ritsumeikan University , Kusatsu, Shiga , Japan.

Theanine (γ-glutamylethylamide) is an amino acid analog that reduces blood pressure and improves immune responses. The ϒ-glutamyltranspeptidase (GGT) from Pseudomonas nitroreducens IFO12694 (PnGGT) has a unique preference for primary amines as ϒ-glutamyl acceptors over standard L-amino acids and peptides. This characteristic is useful for the synthesis of theanine. Read More

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December 2018

Optimising standards of care of heart failure in general practice the OSCAR-HF pilot study protocol.

Acta Cardiol 2018 Dec 3:1-9. Epub 2018 Dec 3.

a Department of Public Health and Primary Care , KU Leuven (KUL) , Leuven , Belgium.

Background: Heart failure (HF) imposes a burden for patients and health economics. General practitioners (GPs) are confronted with the broadest range of HF management. Although guidelines exist, they are not fully implemented in the Belgian health care system. Read More

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December 2018
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Molecular Mechanism of the Pin1-Histone H1 Interaction.

Biochemistry 2018 Dec 3. Epub 2018 Dec 3.

Pin1 is an essential Peptidyl-prolyl isomerase (PPIase) that catalyzes cis-trans prolyl isomerization in proteins containing pSer/Thr-Pro motifs. It has an N-terminal WW domain that targets these motifs and a C-terminal PPIase domain that catalyzes isomerization. Recently, Pin1 was shown to modify the conformation of phosphorylated histone H1 and stabilize the chromatin-H1 interaction by increasing its residence time. Read More

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December 2018

Correlation between Glyoxal-Induced DNA Cross-Links and Hemoglobin Modifications in Human Blood Measured by Mass Spectrometry.

Chem Res Toxicol 2018 Dec 3. Epub 2018 Dec 3.

Glyoxal is an oxoaldehyde generated from the degradation of glucose-protein conjugates and from lipid peroxidation in foods and in vivo, and it is also present in the environment (e.g. cigarette smoke). Read More

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December 2018
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