Virus Res 2017 Jun 12;237:27-36. Epub 2017 May 12.

Department of Pediatrics, Affiliated Hospital of Yanan University, China. Electronic address:

Enterovirus71(EV71), the etiological agent of hand-foot-and-mouth disease, has increasingly become a public health challenge around the world. Type I interferons (IFNs) are an important family of cytokines that regulate innate and adaptive immune responses to pathogens.These pathways are tightly regulated by the host to prevent an inappropriate cellular response, but viruses can modulate these pathways to proliferate and spread. Read More

Arch Virol 2017 Apr 25. Epub 2017 Apr 25.

Department of Pediatrics, The Affiliated Hospital of Qingdao University, No. 59, Haier Road, Qingdao, 266000, China.

The 2'5'-oligoadenylate synthetase (OAS) is an interferon (IFN)-induced protein that plays an important role in the antiviral action of IFN, with OAS3 being one of the four OAS classes (OAS1, OAS2, OAS3, OASL). The effect of OAS on several infectious viral diseases has been reported; however, a study of the effect of OAS3 on enterovirus 71 (EV71) is lacking. The purpose of this study was to evaluate the association of the OAS3 rs1859330 G/A genetic polymorphism with susceptibility and severity of EV71 infection. Read More

Sci Rep 2017 Apr 19;7:45630. Epub 2017 Apr 19.

Laboratory Medicine Center, Children's Hospital, Chongqing Medical University, Chongqing, 400014, China.

Epidemiology and etiology of hand, foot, and mouth disease (HFMD) based on large sample size or evaluation of detection for more enterovirus serotypes are not well investigated in Chongqing of China. 45,616 suspect HFMD patients were prospectively enrolled among whom 21,615 were laboratory confirmed HFMD cases over a 5-year period (January 2011 to December 2015). Their epidemiological, clinical, and laboratory data were extracted and stratified by month, age, sex, disease severity, and enterovirus serotype. Read More

Exp Ther Med 2017 Apr 7;13(4):1403-1407. Epub 2017 Feb 7.

Department of First Pediatric Internal Medicine, Cangzhou Central Hospital, Cangzhou, Hebei 061000, P.R. China.

The aim of this study was to examine the effects of phentolamine on severe hand, foot and mouth disease (HFMD) combined with pulmonary edema (PE). From May 2008 to December 2012, 53 children with severe HFMD plus PE were enrolled in the treatment group, receiving phentolamine intravenously at a loading dose of 5 µg/kg/min. The control group comprised 52 children with the same disease who did not receive phentolamine infusion. Read More

Exp Ther Med 2017 Feb 23;13(2):364-377. Epub 2016 Dec 23.

Department of Clinical Virology, School of Medicine, University of Crete, Heraklion 71003, Greece.

The '2nd Workshop on Paediatric Virology', which took place on Saturday the 8th of October 2016 in Athens, Greece, provided an overview on recent views and advances on Paediatric Virology. Emphasis was given to HIV-1 management in Greece, a country under continuous financial crisis, hepatitis B vaccination in Africa, treatment options for hepatitis C virus in childhood, Zika virus in pregnancy and infancy, the burden of influenza on childhood, hand-foot-mouth disease and myocarditis associated with Coxsackie viruses. Other general topics covered included a critical evaluation of Paediatric Accident and Emergency viral infections, multimodality imaging of viral infections in children, surgical approaches of otolaryngologists to complex viral infections, new advances in the diagnosis and treatment of viral conjunctivitis and novel molecular diagnostic methods for HPV in childhood. Read More

Cutis 2017 Jan;99(1):33-36

School of Public Affairs and Administration and the Departments of Dermatology, Pathology, and Pediatrics, Rutgers University New Jersey Medical School, Newark, USA.

Onychomadesis is the proximal separation of the nail plate from the nail matrix due to a temporary cessation of nail growth. It can occur in children of all ages including neonates. Diagnosis is made clinically through palpation and inspection of the nail plate. Read More

JAAD Case Rep 2017 Jan 2;3(1):49-52. Epub 2017 Feb 2.

Department of Surgery, Section of Dermatology, Dartmouth Hitchcock Medical Center, Lebanon, New Hampshire.

Virus Genes 2017 Jun 7;53(3):352-356. Epub 2017 Feb 7.

BGI-Shenzhen, Shenzhen, 518083, China.

Enterovirus 96 (EV-96) is a recently described member of the species Enterovirus C and is associated with paralysis and myelitis. In this study, using metagenomic sequencing, we identified a new enterovirus 96 strain (EV-96-SZ/GD/CHN/2014) as the sole pathogen causing hand, foot, and mouth disease (HFMD). A genomic comparison showed that EV-96-SZ/GD/CHN/2014 is most similar to the EV-96-05517 strain (85% identity), which has also been detected in Guangdong Province. Read More

Medicine (Baltimore) 2017 Jan;96(4):e5827

aDepartment of Pharmacy, Kaohsiung Chang Gung Memorial Hospital, Taiwan bDepartment of Pediatrics and Kawasaki Disease Center, Kaohsiung Chang Gung Memorial Hospital and Chang Gung University College of Medicine, Taiwan cDepartment of Public Health, College of Health Sciences, Kaohsiung Medical University, Kaohsiung, Taiwan.

Infants who are exposed to the rhinovirus or respiratory syncytial virus are at a higher risk of subsequently developing wheezing or asthma. This study aims to determine whether preschoolers with a history of symptomatic enterovirus infection are at an increased risk of developing allergic diseases or not.We used data from the Taiwan National Health Insurance Research Database from 1999 to 2006 for this nationwide population-based cohort study. Read More

Pediatr Infect Dis J 2017 May;36(5):530-532

From the *URMITE " Unité de Recherche en Maladies Infectieuses et Tropicales Emergentes", UM63, Inserm 1095, CNRS 7278, IRD 198, Institut Hospitalo-Universitaire Méditerranée-Infection, Aix-Marseille University, Marseille, France; †Childcare Centers, Nice, France; ‡Laboratoire de Virologie, Centre National de Référence des Enterovirus et Parechovirus-laboratoire associé, CHU de Clermont-Ferrand, Clermont-Ferrand, France; §Department of Pediatric Cardiac Surgery, La Timone Children's Hospital, Aix-Marseille University, Marseille, France; ¶Department of Pediatric Emergency Medicine, North Hospital, Marseille, France; ‖Department of Pediatrics, Foundation Lenval Children's Hospital, Nice, France; **Laboratory of Foundation Lenval Children's Hospital, Nice, France; and ††IRD French Institute of Research and Development, EHESP French School of Public Health, EPV UMR_D 190 "Emergence des Pathologies Virales," Institut Hospitalo-Universitaire Méditerranée-Infection, Aix Marseille Université, Marseille, France.

We report the investigation methods for the diagnosis of an epidemic and culture-negative Kingella kingae endocarditis complicating a severe outbreak of hand, foot and mouth disease in a childcare center. The diagnosis was confirmed by polymerase chain reaction testing performed from cardiac tissue. Our findings argue for the systematic investigation of K. Read More

Indian J Pediatr 2017 Mar 12;84(3):216-218. Epub 2016 Dec 12.

Department of Pediatrics, Vinayaka Missions Medical College, Karaikal, Puducherry, 609609, India.

Objective: To describe the clinical profile of Hand Foot and Mouth Disease (HFMD) like illness in a cluster of cases.

Methods: Children presenting with papulovesicular skin lesions with or without oral lesions were clinically examined, treated and followed up.

Results: Out of 21 cases, 18 (85. Read More

Biomed Res Int 2016 20;2016:5697571. Epub 2016 Oct 20.

Department of Pediatrics, The Second Affiliated Hospital of Wenzhou Medical University, Wenzhou, China.

This study aimed to evaluate the clinical efficacy and safety of using the traditional Chinese herbal medicine Scutellaria baicalensis for the treatment of severe HFMD in 725 patients aged >1 year in a multicenter, retrospective analysis. The patients were divided into the S. baicalensis and ribavirin groups, and the temperatures, presence or absence of skin rashes and oral lesions, nervous system (NS) involvement, and viral loads of the patients, as well as the safety of the treatments, were evaluated. Read More

Korean J Pediatr 2016 Oct 17;59(10):395-401. Epub 2016 Oct 17.

Department of Pediatrics, Ulsan University Hospital, University of Ulsan College of Medicine, Ulsan, Korea.

Since the outbreak of the enterovirus 71 (EV71) infection in Malaysia in 1997, large epidemics of EV71 have occurred in the Asia-Pacific region. Many children and infants have died from serious neurological complications during these epidemics, and EV71 infection has become a serious public health problem in these areas. EV71 infection causes hand, foot and mouth disease (HFMD) in children, and usually resolves spontaneously. Read More

PLoS One 2016 2;11(11):e0165659. Epub 2016 Nov 2.

Department of Medical Microbiology, Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, Malaysia.

Enterovirus A71 (EV-A71) is one of the main causative agents of hand, foot and mouth disease (HFMD). Unlike other enteroviruses that cause HFMD, EV-A71 is more frequently associated with severe neurological complications and fatality. To date, no effective licensed antivirals are available to combat EV-A71 infection. Read More

J Clin Virol 2016 Nov 19;84:90-95. Epub 2016 Oct 19.

Institute of Virology and Antiviral Therapy, Jena University Hospital, Jena, Germany.

Infection by Enterovirus A71 (EV-A71) is an important cause of hand, foot, and mouth disease (HFMD). Outbreaks including severe cases with neurological and cardiopulmonary complications have been reported particularly from Southeast Asia. In Europe, the epidemiology of EV-A71 is not well understood. Read More

Infect Dis (Lond) 2017 Apr 29;49(4):312-314. Epub 2016 Sep 29.

c Department of Infectious Diseases and Dermatology , Xuhui Central Hospital , Shanghai , China.

BMC Infect Dis 2016 Sep 29;16(1):524. Epub 2016 Sep 29.

State Key Laboratory of Infectious Disease Prevention and Control, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, 102206, China.

Background: Literature shows inconsistency in meteorological effects on Hand, foot, and mouth disease (HFMD) in different cities. This multi-city study aims to investigate the meteorological effects on pediatric HFMD occurrences and the potential effect modification by geographic factors.

Methods: Based on daily time-series data in eight major cities in Guangdong, China during 2009-2013, mixed generalized additive models were employed to estimate city-specific meteorological effects on pediatric HFMD. Read More

J Med Virol 2017 May 11;89(5):801-808. Epub 2016 Oct 11.

Faculty of Medicine, Department of Pediatrics, Center of Excellence in Clinical Virology, Chulalongkorn University, Bangkok, Thailand.

Human rhinovirus (RV) most often causes mild upper respiratory tract infection. Although RV is routinely isolated from the respiratory tract, few studies have examined RV in other types of clinical samples. The prevalence of RV was examined in 1,294 stool samples collected mostly from children with acute gastroenteritis residing in Bangkok and Khon Kaen province of Thailand between January 2010 and October 2014. Read More

Arch Virol 2016 Dec 21;161(12):3463-3471. Epub 2016 Sep 21.

Department of Pediatric Infectious Diseases, Children's Hospital of Dongguan, Dongguan, Guangdong, China.

Enteroviruses (EVs) are the etiological agents involved in most cases of hand, foot and mouth disease (HFMD) and herpangina (HA). Information on the epidemiology profiles of EVs in China is very limited, as the present surveillance system of China focuses on CAV16 and EV71, and no published data are available in Dongguan. The aim of this study is to determine the prevalence of EVs among patients with HFMD and HA in Dongguan, China, during 2015. Read More

Arch Virol 2016 Nov 12;161(11):3073-80. Epub 2016 Aug 12.

Department of Pediatric Infection, The Second Affiliated Hospital of Wenzhou Medical University, 109th Xueyuanxi Road, Wenzhou, Zhejiang, China.

Hand, foot, and mouth disease (HFMD) is a common infectious enterovirus disease, occurring mostly in infants and children younger than 7 years with potentially fatal complications. Therefore, we evaluated the clinical efficacy and safety of recombinant human interferon (IFN)-α2b spray for treating mild HFMD in 400 patients in a randomized, open, controlled clinical trial. The patients were randomized to the IFN-α2b spray and placebo groups, and their temperature, skin rash, oral lesions, and appetite were monitored, while pathogen levels and safety were evaluated with a 7-day follow-up. Read More

BMC Infect Dis 2016 Aug 9;16:399. Epub 2016 Aug 9.

Laboratory of Emerging Infectious Diseases and Division of Laboratory Medicine, Zhujiang Hospital, Southern Medical University, No. 253 Gong ye da dao zhong, Guangzhou, 510282, People's Republic of China.

Background: Enterovirus (EV)-related hand, foot, and mouth disease/herpangina (HFMD/HA) has been prevalent in Guangdong Province, China, since 2010.

Methods: Clinical data for EV-related HFMD/HA inpatients admitted to the Department of Paediatrics of Zhujiang Hospital from 2010 to 2013 were retrospectively reviewed. The corresponding EV serotypes were also determined by reverse transcription-polymerase chain reaction or BLAST analysis of the sequenced partial lengths of the viral protein1/5'-untranslated region. Read More

Chin J Integr Med 2016 Jul 9. Epub 2016 Jul 9.

Department of Pediatrics, Kaifeng Children's Hospital, Kaifeng, Henan Province, 475003, China.

Objective: To evaluate the effificacy and safety of Chinese medicine (CM) plus Western medicine (WM) in the treatment of pediatric patients with severe hand, foot and mouth disease (HFMD) by conducting a prospective, controlled, and randomized trial.

Methods: A total of 451 pediatric patients with severe HFMD were randomly assigned to receive WM therapy alone (224 cases, WM therapy group) or CM [Reduning Injection ( ) or Xiyanping Injection ()] plus WM therapy (227 cases, CM plus WM therapy group) for 7-10 days, according to a web-based randomization system. The primary outcome was fever clearance time, which was presented as temperature decreased half-life time. Read More

Virol J 2016 Jun 24;13:111. Epub 2016 Jun 24.

Department of Microbiology and Immunology, Yong Loo Lin School of Medicine, National University of Singapore, Centre for Life Sciences, 28 Medical Drive, #03-05, Singapore, 117456, Singapore.

Background: Recurrence of hand, foot and mouth disease (HFMD) pandemics continues to threaten public health. Despite increasing awareness and efforts, effective vaccine and drug treatment have yet to be available. Probiotics have gained recognition in the field of healthcare worldwide, and have been extensively prescribed to babies and young children to relieve gastrointestinal (GI) disturbances and diseases, associated or not with microbial infections. Read More

Cutis 2016 May;97(5):E20-1

Department of Dermatology, Kaohsiung Veterans General Hospital, Taiwan.

Singapore Med J 2017 Apr 1;58(4):189-195. Epub 2016 Jun 1.

Khoo Teck Puat-National University Children's Medical Institute, National University Health System, Singapore.

Introduction: Enterovirus infections in childhood can be associated with significant neurological morbidity. This study aimed to describe the prevalence and range of neurological manifestations, determine the clinical characteristics and assess differences in clinical outcomes for Singaporean children diagnosed with enterovirus infections.

Methods: In this single-centre, case-control study, clinical data was collected retrospectively from patients admitted to National University Hospital, Singapore, from August 2007 to October 2011 and diagnosed with enterovirus infection, based on the enterovirus polymerase chain reaction test, or cultures from throat and rectal swabs or cerebrospinal fluid samples. Read More

J Pediatr 2016 Jul 2;174:274. Epub 2016 May 2.

Department of Pediatrics, Ministry of Health Denizli State Hospital, Denizli, Turkey.

Front Pharmacol 2016 29;7:82. Epub 2016 Mar 29.

Department of Pediatrics, National Cheng Kung University Hospital, College of Medicine, National Cheng Kung UniversityTainan, Taiwan; Department of Emergency Medicine, National Cheng Kung University Hospital, College of Medicine, National Cheng Kung UniversityTainan, Taiwan; Center of Infectious Disease and Signaling Research, National Cheng Kung UniversityTainan, Taiwan.

Enterovirus 71 (EV71) was implicated in a widespread outbreak of hand-foot-and-mouth disease (HFMD) across the Asia Pacific area since 1997 and has also been reported sporadically in patients with brain stem encephalitis. Neurogenic shock with pulmonary edema (PE) is a fatal complication of EV71 infection. Among inotropic agents, milrinone is selected as a therapeutic agent for EV71- induced PE due to its immunopathogenesis. Read More

Sci Rep 2016 Apr 7;6:24149. Epub 2016 Apr 7.

Division of Pediatric Infectious Diseases, Department of Pediatrics, Chang Gung Memorial and Children's Hospital, Linkuo, Taiwan.

Human enterovirus 71 (EV71) is a major causative agent of hand, foot, and, mouth disease, accounting for more than 65% of recent outbreaks. Following enteroviral infection, the host responses are crucial indicators for the development of a diagnosis regarding the clinical severity of EV71 infections. In this study, we implemented NanoString nCounter technology to characterize the responses of serum microRNA (miRNA) profiles to various EV71 infection diseases. Read More

Jpn J Infect Dis 2017 Jan 18;70(1):105-107. Epub 2016 Mar 18.

Department of Pediatrics, Nantan General Hospital.

Saffold cardiovirus (SAFV), first identified in a stool sample in 2007, is thought to be associated with respiratory disease and gastroenteritis. On the other hand, animal experiments suggested that the major viral load, following intraperitoneal inoculation of SAFV in mice, may be detected in the pancreas. However, until now, no cases of SAFV in patients with pancreatitis have been reported. Read More

BMC Infect Dis 2016 Mar 16;16:128. Epub 2016 Mar 16.

Laboratory of Emerging Infectious Diseases and Division of Laboratory Medicine, Zhujiang Hospital, Southern Medical University, No. 253 Gong Ye Da Dao Zhong, Guangzhou, 510282, China.

Background: A series of complications caused by enteroviruses, including meningitis, encephalitis, acute flaccid paralysis, acute cardiopulmonary failure, respiratory infection, and myocardial injury have been reported in hand, foot and mouth disease/herpangina (HFMD/HA). However, the complication of diarrhoea caused by enteroviruses has been neglected, and a summary of its clinical features and impact on HFMD/HA is unavailable.

Methods: We included inpatients with HFMD/HA admitted to the Paediatric Department of Zhujiang Hospital during 2009-2012. Read More

Diagn Microbiol Infect Dis 2016 May 5;85(1):36-41. Epub 2016 Feb 5.

Shanghai Rendu Biotechnology Co., Ltd., No. 590 Ruiqing Road, East Area ZhangjiangHigh-Tech Park, Pudong Shanghai, 201201, China.

Hand, foot, and mouth disease (HFMD) is a common infectious disease caused by a group of the human enteroviruses (HEV), including coxsackievirus A16 (CA16) and enterovirus 71 (EV71). In recent years, another HEV-A serotype, CA6 or CA10, has emerged to be one of the major etiologic agents that can induce HFMD worldwide. The objective of this study is to develop specific, sensitive, and rapid methods to help diagnose HEV and CA16 specifically by using simultaneous amplification testing (SAT) based on isothermal amplification of RNA and real-time detection of fluorescence technique, which were named as SAT-HEV and SAT-CA16, respectively (SAT-HEV/SAT-CA16). Read More

J Emerg Med 2016 May 15;50(5):769-72. Epub 2016 Feb 15.

Department of Emergency Medicine, Lehigh Valley Hospital and Health Network/University of South Florida, Morsani College of Medicine, Allentown, Pennsylvania.

Background: Acute cerebellar ataxia is a clinical syndrome with sudden onset of uncoordinated gait and normal mental status in young children. Although it has a benign clinical course, it often requires an exhaustive diagnostic work-up in order to rule out potentially life-threatening etiologies that present similarly. The wide differential encompasses causes from infections, brain masses, drugs, toxins, trauma, paraneoplastic syndromes, as well as hereditary or congenital disorders. Read More

Vaccine 2016 Feb 6;34(7):974-80. Epub 2016 Jan 6.

Institute of Health Policy and Management, National Taiwan University, Taipei, Taiwan. Electronic address:

Objectives: This study aimed to assess the disease burden and economic impacts of human nonpolio enteroviruses (NPEV) and enterovirus A71 (EV-A71) infection in Taiwan.

Materials And Methods: We included children under five years old (n=983,127-1,118,649) with ICD-9-CM codes 0740 (herpangina) or 0743 (hand-foot-and-mouth disease) from the 2006 to 2010 National Health Insurance Database. Severity of enterovirus infection was assessed from outpatient/emergency visits, hospitalization (with/without intensive care unit [ICU] admission), infection with severe complications, and death. Read More

Natl Med J India 2015 May-Jun;28(3):126-8

Department of Paediatrics, Sri Guru Ram Rai Institute of Medical & Health Sciences, Dehradun, Uttarakhand, India.

Background: Hand-foot-mouth disease (HFMD) is known to cause outbreaks around the world as well as in India. We report the clinical characteristics of an outbreak in Dehradun, Uttarakhand.

Methods: The study was done in the Department of Paediatrics of a tertiary care referral teaching hospital in northern India. Read More

Zhonghua Er Ke Za Zhi 2015 Aug;53(8):610-5

Objective: To analyze the genotype, epidemic pattern and the characteristics of the disease of enteroviruses during the epidemic season of hand, foot and mouth disease (HMFD) in children from 2013 to 2014 in Beijing to provide the scientific evidence for prevention and treatment of HFMD.

Method: During April to September in 2013 and March to October in 2014, a total of 977 throat swabs were collected from children who visited the Children's Hospital Affiliated to Capital Institute of Pediatrics, including 147 from patients with HFMD in 2013, 343 with HFMD, 201 with atypical HFMD, 83 with herpangina, 25 with fever with convulsions, 64 fever with rash and 114 with rash in 2014. Enteroviruses universal type (EV), Enteroviruses type 71 (EV71) and Coxsackievirus group A 16 (CA16) were detected by real-time RT-PCR respectively. Read More

Shock 2016 Jun;45(6):620-5

*Department of Emergency Medicine, National Taiwan University Hospital Yunlin Branch, Douliou †Department of Emergency Medicine, National Taiwan University Hospital, Taipei, Taiwan ‡Department of Critical Care Medicine, Hunan Children's Hospital §Department of Pediatrics, Hunan Provincial People's Hospital, Changsha, Hunan Province, China.

Objective: Our goal is to determine the prognostic value of serum N-terminal prohormone of brain natriuretic peptide (NT-proBNP), leukocytosis, and hyperglycemia in patients with severe hand, foot, and mouth disease (HFMD).

Design: This is a prospective cohort study conducted from March 2011 through October 2012 at Hunan Children's Hospital.

Setting: Hunan Children's Hospital, a large children's teaching hospital with 1,500-beds located in the Changsha region of Hunan Province in China. Read More

Curr Opin Pediatr 2016 Feb;28(1):107-13

aDepartment of Pediatrics bMolecular and Medical Pharmacology, David Geffen School of Medicine at UCLA, University of California, Los Angeles, California, USA.

Purpose Of Review: Enteroviruses cause a wide variety of diseases with neurologic, respiratory, skin, and gastrointestinal findings. The purpose of this review is to clarify changes in the classification of enteroviruses, provide information about recent disease outbreaks, and to summarize progress toward the treatment and prevention of these infections.

Recent Findings: Enteroviruses are now classified into four distinct species. Read More

Biomed Res Int 2015 26;2015:802046. Epub 2015 Nov 26.

Department of Infectious Disease, Children's Hospital of Chongqing Medical University, Ministry of Education Key Laboratory of Child Development and Disorders, Chongqing Key Laboratory of Pediatrics, Chongqing 400014, China.

Background: Hand-foot-and-mouth disease (HFMD) is a disease that had similar manifestations to chickenpox, impetigo, and measles, which is easy to misdiagnose and subsequently causes delayed therapy and subsequent epidemic. To date, no study has been conducted to report the clinical and epidemiological characteristics of atypical HFMD.

Methods: 64 children with atypical HFMD out of 887 HFMD children were recruited, stool was collected, and viral VP1 was detected. Read More

Epidemiol Infect 2016 May 23;144(7):1512-9. Epub 2015 Nov 23.

Institute of Occupational Medicine and Industrial Hygiene, National Taiwan University College of Public Health,Taipei,Taiwan.

The community burden of enterovirus is often monitored through syndromic monitoring systems based on reported cases of enterovirus-related infection (EVI) diagnoses. The extent to which this is affected by under- and over-diagnosis has not been reported. In Taiwan, children often make more than one healthcare visit during an episode of infection. Read More

Indian Pediatr 2015 Aug;52(8):716

Department of Pediatrics, JIPMER, Puducherry, India.

Virus Genes 2015 Oct 25;51(2):290-3. Epub 2015 Aug 25.

Department of Pediatrics, Center of Excellence in Clinical Virology, Faculty of Medicine, Chulalongkorn University, Bangkok, 10330, Thailand.

The complete genomic sequences of 14 enterovirus 71 (EV71) strains isolated from children with hand, foot, and mouth disease in Thailand from 2012 to 2014 were determined and compared to enterovirus group A prototypes. Phylogenetic analysis revealed that 13 strains resembled the B5 subgroup, while one strain from a fatal case designated THA_1219 belonged to the C4 subgroup. Similarity plot and bootscan analyses suggested that THA_1219 underwent recombination in the P2 and P3 regions. Read More

J Med Virol 2016 Mar 9;88(3):408-16. Epub 2015 Nov 9.

Department of Infectious Diseases, Children's Hosptital of Chongqing Medical University, Yuzhong District, Chongqing, China.

Hand, foot, and mouth disease (HFMD) has become very common in children, with widespread occurrence across China. The aim of this study was to investigate the epidemiologic and etiologic characteristics of HFMD, including etiologic variations in Chongqing, China. An epidemiologic investigation was based on 3,472 patients who presented with HFMD manifestations and were admitted at the Children's Hospital of Chongqing Medical University between 2010 and 2013. Read More

Springerplus 2015 17;4:362. Epub 2015 Jul 17.

Department of Pediatrics, Faculty of Medicine, Center of Excellence in Clinical Virology, Chulalongkorn University, Bangkok, 10330 Thailand.

Background: Hand, food, and mouth disease (HFMD) is a highly contagious disease caused by enteroviruses infection. It is a health problem in young children under 5 years of age worldwide. The common causative agents are coxsackievirus A 16 (CA16) and enterovirus 71 (EV71). Read More

Pediatr Emerg Care 2017 Apr;33(4):230-233

From the *Department of Emergency Medicine, Royal Children's Hospital; †Murdoch Children's Research Institute; ‡Department of Paediatrics, Faculty of Medicine, Dentistry, and Health Sciences, University of Melbourne; and §Junior Medical Staff Department, St Vincent's Hospital, Victoria, Australia.

Objective: The aim of the study was to explore physician perceptions of the amount of fluid that demonstrates a successful "trial of fluids" (adequate fluid intake) in the emergency department in children who have had insufficient fluid intake at home.

Methods: This is a secondary analysis of a randomized placebo-controlled trial of viscous lidocaine versus placebo in children aged 6 months to 8 years with acute infectious ulcerative mouth conditions (gingivostomatitis, ulcerative pharyngitis, or hand foot and mouth disease) and poor oral fluid intake. We measured the amount of fluid ingested in 60 minutes after administration of the intervention and related physician perception of adequate intake to measured intake. Read More

Exp Ther Med 2015 Jun 3;9(6):2213-2218. Epub 2015 Apr 3.

Department of Pediatrics, Shilong People's Hospital, Dongguan, Guangdong 523320, P.R. China.

Immune reaction plays a crucial role in the regulation of the progression of Coxsackievirus A16 (CA16)-infected hand, foot and mouth disease (HFMD). However, no details of T-cell subset frequency or imbalance during the CA16 infection process have been revealed. In the present study, whether CA16-induced HFMD changes the frequency of different T-cell subsets and associated immune mediators was determined in children. Read More

Sci Rep 2015 Jun 30;5:11700. Epub 2015 Jun 30.

Department of Dermatology, Xinhua Hospital Affiliated to Shanghai Jiaotong University School of Medicine, Shanghai, China.

Since late 2012, coxsackievirus A6 (CVA6) has gradually become the predominant pathogen responsible for hand-foot-mouth disease (HFMD) in several provinces of China. A total of 626 patients diagnosed with HFMD in Shanghai, China from January 2012 to September 2013 were enrolled in this study. Of these, 292 CVA6 infected cases were subjected to clinical analyses. Read More

J Pediatr 2015 Jul;167(1):85

McMaster University, Hamilton, Ontario, Canada.

Zhongguo Dang Dai Er Ke Za Zhi 2015 Jun;17(6):602-6

Department of Pediatrics, Guilin People's Hospital, Guilin, Guangxi 541002, China.

Objective: To study the clinical values of basic vital signs in early identification of critical hand-foot-mouth disease (HFMD).

Methods: The clinical data of 358 children with severe HFMD [212 cases in stage 2 (central nervous system involvement) and 146 cases in stage 3 (earlier stage of cardiopulmonary failure, critical type)] were reviewed. The diagnostic values of peak temperature and duration of fever, as well as the heart rate (HR), respiratory rate (RR), systolic blood pressure (SBP), and diastolic blood pressure (DBP) in different age groups, for critical HFMD (stage 3) were analyzed using the receiver operating characteristic (ROC) curve. Read More

Curr Opin Pediatr 2015 Aug;27(4):486-91

aDepartment of Pediatrics bDepartment of Dermatology, University of North Carolina Hospitals, Chapel Hill, North Carolina, USA.

Purpose Of Review: Hand-foot-and-mouth disease (HFMD) is a common cause of viral rash in children with classic skin findings which are easily recognized by pediatricians. Recently, several atypical cutaneous manifestations of HFMD have been described. Awareness of these patterns may lead providers to appropriate diagnosis and management. Read More

Emerg Microbes Infect 2014 May 21;3(5):e35. Epub 2014 May 21.

Department of Biomedical Sciences and Pathobiology, Virginia-Maryland Regional College of Veterinary Medicine, Virginia Polytechnic Institute and State University , Blacksburg, VA 24061-0913, USA.

Vaccine development and pathogenesis studies for human enterovirus 71 are limited by a lack of suitable animal models. Here, we report the development of a novel neonatal gnotobiotic pig model using the non-pig-adapted neurovirulent human enterovirus 71 strain BJ110, which has a C4 genotype. Porcine small intestinal epithelial cells, peripheral blood mononuclear cells and neural cells were infected in vitro. Read More