300,158 results match your criteria Oxidative medicine and cellular longevity[Journal]


Nonalcoholic-Fatty-Liver-Disease and Nonalcoholic Steatohepatitis: Successful Development of Pharmacological Treatment Will Depend on Translational Research.

Digestion 2018 Dec 7:1-7. Epub 2018 Dec 7.

Background: Nonalcoholic-fatty-liver-disease/nonalcoholic steatohepatitis (NAFLD/NASH) is expected to become the leading liver disease worldwide. Typical liver-related complications are fibrosis, cirrhosis, and the development of hepatocellular cancer (HCC) with the need for liver transplantation. Up to now there is no approved pharmacotherapy. Read More

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December 2018

Establishment of a Mouse Model of Premature Ovarian Failure Using Consecutive Superovulation.

Cell Physiol Biochem 2018 7;51(5):2341-2358. Epub 2018 Dec 7.

Key Laboratory of the Model Animal Research, Animal Core Facility of Nanjing Medical University, Nanjing Medical University, Nanjing,

Background/aims: This study investigated the effect of consecutive superovulation on the ovaries and established a premature ovarian failure (POF) model in mice.

Methods: The mouse POF model was induced by 5-15 consecutive superovulation treatments with pregnant mare serum gonadotropin (PMSG), human chorionic gonadotropin (HCG) and prostaglandin F2α (PGF2α). Normal adult mice were compared with mice displaying natural ovarian aging. Read More

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December 2018

Inhibition of Glutathione Synthesis via Decreased Glucose Metabolism in Stored RBCs.

Cell Physiol Biochem 2018 7;51(5):2172-2184. Epub 2018 Dec 7.

Department of Immunology, Binzhou Medical University, Yantai,

Background/aims: Although red blood cells (RBCs) transfusions can be lifesaving, they are not without risk. RBCs storage is associated with the abnormal metabolism of glutathione (GSH), which may increase the risk of the oxidative damage of RBCs after transfusion. The responsible mechanisms remain unknown. Read More

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December 2018

In Vivo Assessment of Combined Effects of Glibenclamide and Losartan in Diabetic Rats.

Med Princ Pract 2018 Dec 10. Epub 2018 Dec 10.

Objective: Diabetic complications involve multiple pathological pathways, including hyperglycemia-induced oxidative stress and inflammation. Combination therapy is usually employed to improve treatment outcomes and to lower potential adverse effects. In this study, we evaluated the effects of antidiabetic and antihypertensive agents, glibenclamide (GLI) and losartan (LT), on diabetes-associated metabolic changes in rats. Read More

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December 2018

Pseudoginsenoside-F11 attenuates cognitive impairment by ameliorating oxidative stress and neuroinflammation in d‑galactose-treated mice.

Int Immunopharmacol 2018 Dec 8;67:78-86. Epub 2018 Dec 8.

Department of Pharmacology, Shenyang Pharmaceutical University, Shenyang, PR China. Electronic address:

Oxidative stress and neuroinflammation are thought to be the two key early events during the process of mild cognitive impairment (MCI). Therefore, effective regulation of oxidative stress and neuroinflammation is an important aspect of preventing and improving MCI. We previously found that pseudoginsenoside-F11 (PF11), an ocotillol-type saponin, markedly reduced cognitive impairment in APP/PS1 mice and oAβ-injected mice. Read More

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December 2018

Cadmium induces apoptotic program imbalance and cell cycle inhibitor expression in cultured human astrocytes.

Environ Toxicol Pharmacol 2018 Dec 3;65:53-59. Epub 2018 Dec 3.

Department of Pharmacology, Faculty of Science, Mahidol University, Bangkok, Thailand; Translational Medicine Graduate Program, Faculty of Medicine Ramathibodi Hospital, Mahidol University, Bangkok, Thailand. Electronic address:

Cadmium is a highly neurotoxic heavy metal impairing neurogenesis and induces neurodegenerative disorders. Toxic concentrations of cadmium induce astrocytic apoptosis by depleting intracellular glutathione levels, elevating intracellular calcium levels, altering mitochondria membrane potentials, and activating JNK and PI3K/Akt signaling pathways. Cadmium suppresses cell proliferation in kidney epithelial cells, lung fibroblasts, and primary myelocytes; however, cadmium's effects on proteins regulating oxidative stress, apoptosis, and cell proliferation in astrocytes are less known. Read More

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December 2018

cDNA cloning and expression analysis of glutaredoxin (Grx) 2 in the Pacific white shrimp Litopenaeus vannamei.

Fish Shellfish Immunol 2018 Dec 8. Epub 2018 Dec 8.

Hainan Provincial Key Laboratory for Functional Components Research and Utilization of Marine Bio-resources, Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou, 571101, People's Republic of China. Electronic address:

Glutaredoxin (Grx) is a class molecule oxidoreductase, which can regulate the redox state of proteins and plays a key role in antioxidant defense. However, the informations of Grx cDNA sequences and their functions are lack in decapod crustacea. In the present study, the cDNA of LvGrx 2 was cloned from the Pacific white shrimp, Litopenaeus vannamei. Read More

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December 2018

Protein S-glutathionylation: The linchpin for the transmission of regulatory information on redox buffering capacity in mitochondria.

Chem Biol Interact 2018 Dec 8. Epub 2018 Dec 8.

Protein S-glutathionylation reactions are a ubiquitous oxidative modification required to control protein function in response to changes in redox buffering capacity. These reactions are rapid and reversible and are, for the most part, enzymatically mediated by glutaredoxins (GRX) and glutathione S-transferases (GST). Protein S-glutathionylation has been found to control a range of cell functions in response to different physiological cues. Read More

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December 2018

Effects of Carvacrol, Thymol and essential oils containing such monoterpenes on wound healing: a systematic review.

J Pharm Pharmacol 2018 Dec 7. Epub 2018 Dec 7.

Programa de Pós-graduação em Ciências Aplicadas à Saúde, Universidade Federal de Sergipe, Lagarto, Sergipe, Brazil.

Objectives: The treatment of wounds accounts for a considerable fraction of health expenses as well as serious socioeconomic problems. The use of natural substances stands out as a source of new therapeutic discoveries for the wound healing. Thus, this review compiled scientific findings on the applicability of carvacrol and thymol, or essential oils containing at least one of these compounds, for the treatment of wounds. Read More

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December 2018
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Combination of Whole Grape Seed Flour and Newly Isolated Kefir Lactic Acid Bacteria Reduces High-Fat (HF)-induced Hepatic Steatosis.

Mol Nutr Food Res 2018 Dec 11:e1801040. Epub 2018 Dec 11.

J.-H. Kwon, H. G. Lee, K.-H. Seo, H. Kim, Department of Food and Nutrition, Hanyang University, Seoul.

Scope: This study aimed to determine the separate and combined effect of a prebiotic (Catechin-rich wine grape seed flour, GSF) and a probiotic (newly isolated kefir lactic acid bacteria, LAB) on hepatic steatosis of obese mice.

Methods And Results: High-fat (HF)-induced obese mice were fed HF diet containing 6% microcrystalline cellulose (MCC, control), 10% GSF, orally administrated LAB, or combination of GSF and LAB for 9 weeks. There was a significant reduction of body weight gain, and liver weights, plasma insulin concentrations, and HOMA-IR in all experimental groups compared to control. Read More

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December 2018

Prognostic implications of markers of the metabolic phenotype in human cutaneous melanoma.

Br J Dermatol 2018 Dec 7. Epub 2018 Dec 7.

Instituto de Investigación Hospital 12 de Octubre, Universidad Complutense de Madrid, Madrid, Spain.

Background: Reprogramming of energy metabolism to enhanced aerobic glycolysis has been defined as a hallmark of cancer.

Objective: To investigate the role of the mitochondrial proteins, β-subunit of the H -ATP synthase (β-F1-ATPase), and heat-shock protein 60 (HSP60), and the glycolytic markers, glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and pyruvate kinase M2 (PKM2), as well as the bioenergetic cellular (BEC) index, in melanoma progression.

Material And Methods: The expression of energy metabolism proteins were assessed on a set of different melanoma cells representing the natural biological history of the disease: primary cultures of melanocytes, radial (WM35) and vertical (WM278) growth phases, and poorly (C81-61-PA) and highly (C8161-HA) aggressive melanoma cells. Read More

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December 2018
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Microglial cell activation and senescence are characteristic of the pathology FXTAS.

Mov Disord 2018 Dec 10. Epub 2018 Dec 10.

MIND Institute, UC Davis Medical Center, Sacramento, California, USA.

Background: Fragile X-associated tremor/ataxia syndrome (FXTAS) is a late-onset neurodegenerative disorder associated with premutation alleles of the FMR1 gene. Expansions of more than 200 CGG repeats give rise to fragile X syndrome, the most common inherited form of cognitive impairment. Fragile X-associated tremor/ataxia syndrome is characterized by cerebellar tremor and ataxia, and the presence of ubiquitin-positive inclusions in neurons and astrocytes. Read More

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December 2018
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Downregulation of long noncoding RNA H19 rescues hippocampal neurons from apoptosis and oxidative stress by inhibiting IGF2 methylation in mice with streptozotocin-induced diabetes mellitus.

J Cell Physiol 2018 Dec 7. Epub 2018 Dec 7.

Department of Neurosurgery, The First Hospital of Jilin University, Changchun, China.

The diabetes mellitus (DM)-induced reduction of neurogenesis in the hippocampus is consequently accompanied by cognitive decline. The present study set out to define the critical role played by long noncoding RNA H19 (lncRNA H19) in the apoptosis of hippocampal neurons, as well as oxidative stress (OS) in streptozotocin (STZ)-induced DM mice through regulation of insulin-like growth factor 2 (IGF2) methylation. The expression of lncRNA H19 in the hippocampal neurons and surviving neurons were detected. Read More

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December 2018

The methanolic extract of Guibourtia tessmannii (caesalpiniaceae) and selenium modulate cytosolic calcium accumulation, apoptosis and oxidative stress in R2C tumour Leydig cells: Involvement of TRPV1 channels.

Andrologia 2018 Dec 10:e13216. Epub 2018 Dec 10.

Department of Physiology, School of Medical Sciences, Health Campus, Universiti Sains Malaysia, Kelantan, Malaysia.

This study evaluated the effects of the methanolic extract of Guibourtia tessmannii (GT) and selenium (Se) on cell viability, intracellular calcium concentration ([Ca ] ), apoptosis and oxidative stress through transient receptor potential vanilloid 1 (TRPV1) channel activity in CCL-97 (R2C) tumour Leydig cells. The cells were divided into nine groups and treated as follows: (a)-Control, (b)-Capsazepine (CPZ, 0.1 mM, a TRPV1 channel blocker), (c)-Capsaicin (CAP, 0. Read More

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December 2018

An Oxygen-Vacancy-Rich Semiconductor-Supported Bifunctional Catalyst for Efficient and Stable Zinc-Air Batteries.

Adv Mater 2018 Dec 9:e1806761. Epub 2018 Dec 9.

Department of Chemical Engineering, Waterloo Institute for Nanotechnology, Waterloo Institute for Sustainable Energy, University of Waterloo, Waterloo, Ontario, N2L 3G1, Canada.

The highly oxidative operating conditions of rechargeable zinc-air batteries causes significant carbon-support corrosion of bifunctional oxygen electrocatalysts. Here, a new strategy for the catalyst support design focusing on oxygen vacancy (OV)-rich, low-bandgap semiconductor is proposed. The OVs promote the electrical conductivity of the oxide support, and at the same time offer a strong metal-support interaction (SMSI), which enables the catalysts to have small metal size, high catalytic activity, and high stability. Read More

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December 2018

Protective effects of plant-derived natural products on renal complications.

J Cell Physiol 2018 Dec 7. Epub 2018 Dec 7.

Biotechnology Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, Iran.

Diabetic nephropathy is the leading cause of renal failure worldwide. This debilitating disorder has several underlying pathophysiologic mechanisms, and therefore a variety of pharmacologic agents have been developed to prevent or treat diabetic nephropathy; however, synthetic drugs may possess unfavorable side effects. In response to this, the global use of herbal-based pharmacologic agents is increasing among diabetic patients. Read More

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December 2018

Toll-like receptor 2 regulates metabolic reprogramming in gastric cancer via superoxide dismutase 2.

Int J Cancer 2018 Dec 11. Epub 2018 Dec 11.

Department of General Surgery, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, 200080, China.

Toll-like receptors (TLRs) play critical roles in host defence following recognition of conserved microbial- and host-derived components, and their dysregulation is a common feature of various inflammation-associated cancers, including gastric cancer (GC). Despite the recent recognition that metabolic reprogramming is a hallmark of cancer, the molecular effectors of altered metabolism during tumorigenesis remain unclear. Here, using bioenergetics function assays on human GC cells, we reveal that ligand-induced activation of TLR2, predominantly through TLR1/2 heterodimer, augments both oxidative phosphorylation (OXPHOS) and glycolysis, with a bias towards glycolytic activity. Read More

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December 2018

Effect of Ginkgo biloba seed exopleura extract and chitosan coating on the postharvest quality of Ginkgo seed.

J Sci Food Agric 2018 Dec 10. Epub 2018 Dec 10.

Co-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing, 210037, P. R. China.

Background: The effects of Ginkgo biloba seed exopleura extract (GSEE) and chitosan (CH) coating on the preservation of ginkgo seeds were investigated.

Results: Results showed that chitosan could alleviate the decay rate, and that CH combined with GSEE treatment (CH-GSEE) further inhibited the development of seed decay due to the additional antifungal activity of GSEE. The nutrient contents, including water, starch, and soluble protein, were maintained by both CH-GSEE and CH treatment. Read More

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December 2018

Cationic Pb2 Dumbbells Stabilized in the Highly Covalent Lead Nitridosilicate Pb2Si5N8.

Angew Chem Int Ed Engl 2018 Dec 7. Epub 2018 Dec 7.

Ludwig-Maximilians-Universität München, Department Chemie Lehrstuhl f. Anorganische Festkörperchemie, Butenandtstr. 5-13, 81377, München, GERMANY.

Due to the weak oxidative force of N2, nitrides are only typically formed with the less electronegative metals. Meeting this challenge, we here present Pb2Si5N8, the first nitridosilicate containing highly electron-affine cations of a metal from the right side of the Zintl border. By using advanced synchrotron X-ray diffraction, the crystal structure was determined from a tiny single crystal of 1×3×3 μm3 in size, revealing a significantly different bonding situation compared to all other nitridosilicates known so far. Read More

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December 2018

Coenzyme Q protects against β-cell toxicity induced by pravastatin treatment of hypercholesterolemia.

J Cell Physiol 2018 Dec 7. Epub 2018 Dec 7.

Department of Structural and Functional Biology, Biology Institute, State University of Campinas, Campinas, SP, Brazil.

New onset of diabetes is associated with the use of statins. We have recently demonstrated that pravastatin-treated hypercholesterolemic LDL receptor knockout (LDLr ) mice exhibit reductions in insulin secretion and increased islet cell death and oxidative stress. Here, we hypothesized that these diabetogenic effects of pravastatin could be counteracted by treatment with the antioxidant coenzyme Q (CoQ ), an intermediate generated in the cholesterol synthesis pathway. Read More

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December 2018

Impact of Oxidative Metabolism on the Cytotoxic and Genotoxic Potential of Genistein in Human Colon Cancer Cells.

Mol Nutr Food Res 2018 Nov 22:e1800635. Epub 2018 Nov 22.

Department of Food Chemistry and Toxicology, University of Vienna, Vienna, Austria.

Scope: Genistein (GEN) is known to be genotoxic via targeting topoisomerase-II (TOPII). Oxidative metabolism of GEN is shown to generate hydroxylated metabolites with catecholic structures. The present study focuses on the impact of oxidative metabolism of GEN, exemplified for 3'-hydroxygenistein (3'-OH-GEN) and 6-hydroxygenistein (6-OH-GEN), on topoisomerase interference and the resulting genotoxic potential in HT-29 human colon carcinoma cells. Read More

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November 2018

Dopamine, oxidative stress and protein-quinone modifications in Parkinson's and other neurodegenerative diseases.

Angew Chem Int Ed Engl 2018 Dec 11. Epub 2018 Dec 11.

Universita degli Studi di Pavia, Chimica, Via Taramelli 12, 27100, Pavia, ITALY.

Dopamine (DA) is the most important catecholamine in the brain, as it is the most abundant and the precursor of other neurotransmitters. Degeneration of nigrostriatal neurons of substantia nigra pars compacta in Parkinson's disease represents the best-studied link between DA neurotransmission and neuropathology. Catecholamines are reactive molecules that are handled through complex control and transport systems. Read More

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December 2018

Cardiac metabolic modulation upon low-carbohydrate low-protein ketogenic diet in diabetic rats studied in vivo using hyperpolarized C pyruvate, butyrate, and acetoacetate probes.

Diabetes Obes Metab 2018 Dec 11. Epub 2018 Dec 11.

Functional Metabolism Group, Singapore Bioimaging Consortium, Agency for Science, Technology, and Research, Singapore.

Aims: To investigate the effects of long-term low-carbohydrate low-protein ketogenic diet (KD) on cardiac metabolism and diabetic cardiomyopathy status in lean diabetic Goto-Kakizaki (GK) rats.

Materials And Methods: Diabetic GK rats were fed with KD for 62 weeks. Cardiac function and metabolism were assessed using magnetic resonance imaging and C magnetic resonance spectroscopy ( C-MRS), at rest and under dobutamine stress. Read More

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December 2018

Uric acid and angiotensin II additively promote inflammation and oxidative stress in human proximal tubule cells by activation of toll-like receptor 4.

J Cell Physiol 2018 Dec 10. Epub 2018 Dec 10.

Department of Internal Medicine, University of Genoa and Ospedale Policlinico San Martino-IST, Genova, Italy.

Renal proximal tubular cells (PTECs) participate in several mechanisms of innate immunity, express toll-like receptors (TLRs), and proinflammatory cytokines. Hyperuricemia may be a promoter of inflammation and renal damage. Angiotensin II (Ang II) modulate immune and inflammatory responses in renal tubular cells. Read More

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December 2018

Atrial fibrillation in β-thalassemia patients with a focus on the role of iron-overload and oxidative stress: A review.

J Cell Physiol 2018 Dec 10. Epub 2018 Dec 10.

Department of Clinical Pharmacy, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran.

Cardiac complications including arrhythmia and especially atrial fibrillation (AF) are common causes of death in β-thalassemia patients. The main factor in the etiopathogenesis of these complications is iron overload, which results in increased oxidative stress. Although there is a known association between cardiac complications and iron overload in β-thalassemia patients, there is no comprehensive review on AF and excessive iron with a focus on oxidative stress in these patients. Read More

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December 2018

8:2 Fluorotelomer alcohol causes immunotoxicity and liver injury in adult male C57BL/6 mice.

Environ Toxicol 2018 Dec 7. Epub 2018 Dec 7.

College of Biotechnology and Bioengineering, Zhejiang University of Technology, Hangzhou, China.

8:2 Fluorotelomer alcohol (8:2 FTOH) is widely used in houseware and industrial goods and is ubiquitous in the surrounding environment. 8:2 FTOH has been linked to hepatoxicity, nephrotoxicity, and reproductive toxicity, as well as endocrine-disrupting effects. However, as of yet, the research regarding immunotoxicity of 8:2 FTOH remains largely limited. Read More

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December 2018
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Menadione perturbs oxidative stress biomarkers and testicular function indices of rats.

J Biochem Mol Toxicol 2018 Dec 10:e22282. Epub 2018 Dec 10.

Antioxidants, Redox Biology, and Toxicology Research Laboratory, Department of Medical Biochemistry, College of Health Sciences, Nile University of Nigeria, FCT-Abuja, Nigeria.

In this study, we evaluated the influence of menadione on the androgenic, oxidative stress biomarkers, and testicular function indices in rats. Rats (20) were randomized into four groups (A-D) of five rats each. Rats in groups A, B, C, and D received the vehicle of administration (olive oil), 25, 50, or 100 mg/kg body weight menadione intraperitoneally, respectively, for 7 days. Read More

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December 2018

Study of nuclear factor-2 erythroid related factor-2 activator, berberine, in paclitaxel induced peripheral neuropathy pain model in rats.

J Pharm Pharmacol 2018 Dec 10. Epub 2018 Dec 10.

Department of Pharmacology, Post Graduate Institute of Medical Education & Research (PGIMER), Chandigarh, India.

Objectives: The role of nuclear factor-2 erythroid related factor-2 (Nrf2) activator, berberine (BBR), has been established in rat model of streptozotocin induced diabetic neuropathy. Around 30-40% of cancer patients, on paclitaxel (PTX) chemotherapy develop peripheral neuropathy. The present study was contemplated with the aim of establishing the neuropathy preventive role of BBR, in paclitaxel induced peripheral neuropathy model in rats. Read More

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December 2018

Effect of experimentally induced hypertension on cerebellum of postmenopausal rat.

J Cell Physiol 2018 Dec 7. Epub 2018 Dec 7.

Department of Physiology, Faculty of Medicine, Minia University, Minia, Egypt.

Cerebellum seems to be a specific target for both the decrease of estrogen and hypertension in menopause. The aim of this study was to investigate the hypertension and menopause-induced changes in rat's cerebellar cortex and the possible mechanisms of these changes. Rats were divided into four groups: the sham-operated control (SC-group), the ovariectomized (OVX-group), the hypertensive (H-group), and the ovariectomized-hypertensive (OVX-H-group) group. Read More

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December 2018
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Jobelyn Attenuates Oxidative Stress and Improves Memory Performance in Mice Exposed to Anoxic Stress.

Authors:
Osarume Omorogbe

Drug Res (Stuttg) 2018 Dec 10. Epub 2018 Dec 10.

Neuropharmacology Unit, Department of Pharmacology and Therapeutics, University of Ibadan, Ibadan, Nigeria.

Objective: Chronic stress impairs memory by damaging brain cells via induction of oxidative stress. Hence, the use of agents with antioxidant activity is being investigated as potential drugs against chronic stress-induced memory decline. This study evaluated the effect of Jobelyn (JB), a potent antioxidant food supplement on memory deficit induced by anoxic stress in mice and on brain levels of biomarkers of oxidative stress. Read More

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December 2018

Mitochondrial Neuroglobin Is Necessary for Protection Induced by Conditioned Medium from Human Adipose-Derived Mesenchymal Stem Cells in Astrocytic Cells Subjected to Scratch and Metabolic Injury.

Mol Neurobiol 2018 Dec 8. Epub 2018 Dec 8.

Departamento de Nutrición y Bioquímica, Facultad de Ciencias, Pontificia Universidad Javeriana, Bogotá, D.C., Colombia.

Astrocytes are specialized cells capable of regulating inflammatory responses in neurodegenerative diseases or traumatic brain injury. In addition to playing an important role in neuroinflammation, these cells regulate essential functions for the preservation of brain tissue. Therefore, the search for therapeutic alternatives to preserve these cells and maintain their functions contributes in some way to counteract the progress of the injury and maintain neuronal survival in various brain pathologies. Read More

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December 2018

Inflammasome and Cancer.

Exp Suppl 2018 ;108:281-302

Integrative Breast Cancer Research Laboratory, Guangdong Provincial Hospital of Chinese Medicine, Guangzhou University of Chinese Medicine, Guangzhou, China.

The current chapter focuses on the role of inflammasome in cancer prevention and development. Emerging evidence suggested that inflammasome is closely correlated with elevated levels of IL-1β and IL-18, activation of NF-κB signaling, enhanced mitochondrial oxidative stress, and activation of autophagic process in cancer. Meanwhile, inflammasome component NOD-like receptors (NLRs) are also involved in carcinogenesis and closely correlated to chemoresponse and prognosis. Read More

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January 2018

A phenylboronate-based SERS nanoprobe for detection and imaging of intracellular peroxynitrite.

Mikrochim Acta 2018 Dec 10;186(1):11. Epub 2018 Dec 10.

Key Laboratory for Advanced Materials, Shanghai Key Laboratory of Functional Materials Chemistry, School of Chemistry and Molecular Engineering, East China University of Science and Technology, 130 Meilong Road, Shanghai, 200237, China.

A surface-enhanced Raman scattering (SERS) based nanoprobe was developed for detection and imaging of endogenous peroxynitrite in living cells. The probe was fabricated by assembling 3-mercaptophenylboronic acid pinacol ester onto the surface of gold nanoparticles (AuNPs). The detection of peroxynitrite is accomplished via measurement of the changes in the SERS spectra (at 882 cm) that are caused by the reaction between probe and peroxynitrite. Read More

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December 2018

Mitochondrial membrane transporters and metabolic switch in heart failure.

Heart Fail Rev 2018 Dec 10. Epub 2018 Dec 10.

Cardiovascular Diseases and Diabetes Biology group, Rajiv Gandhi Centre for Biotechnology (RGCB), Poojappura, Thycaud Post, Trivandrum, Kerala, 695014, India.

Mitochondrial dysfunction is widely recognized as a major factor for the progression of cardiac failure. Mitochondrial uptake of metabolic substrates and their utilization for ATP synthesis, electron transport chain activity, reactive oxygen species levels, ion homeostasis, mitochondrial biogenesis, and dynamics as well as levels of reactive oxygen species in the mitochondria are key factors which regulate mitochondrial function in the normal heart. Alterations in these functions contribute to adverse outcomes in heart failure. Read More

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December 2018

Phosphorus Influences the Interaction Between Toxigenic Microcystis and Chloramphenicol.

Bull Environ Contam Toxicol 2018 Dec 7. Epub 2018 Dec 7.

College of Resources and Environmental Sciences, China Agricultural University, Beijing, 100193, China.

Microcystis growth and physiological responses to chloramphenicol (CAP)-stress were explored at different phosphorus (P) concentrations during 20-day exposure. Under CAP-stress, Microcystis exhibited (i) stronger total protein synthesis and antioxidant defenses at 5 mg/L P than 0.05-0. Read More

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December 2018

N-Acetylcysteine protects against intrauterine growth retardation-induced intestinal injury via restoring redox status and mitochondrial function in neonatal piglets.

Eur J Nutr 2018 Dec 10. Epub 2018 Dec 10.

College of Animal Science and Technology, Nanjing Agricultural University, No. 1, Weigang Road, Nanjing, 210095, Jiangsu, People's Republic of China.

Purpose: Intrauterine growth retardation (IUGR) is detrimental to the intestinal development of neonates, yet satisfactory treatment strategies remain limited. This study was, therefore, conducted using neonatal piglets as a model to investigate the potential of N-acetylcysteine (NAC) to alleviate intestinal damage caused by IUGR.

Methods: Seven normal birth weight (NBW) and fourteen IUGR neonatal male piglets were selected and then fed a basal milk diet (NBW-CON and IUGR-CON groups) or a basal milk diet supplemented with 1. Read More

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December 2018

Cytosolic lipid excess-induced mitochondrial dysfunction is the cause or effect of high fat diet-induced skeletal muscle insulin resistance: a molecular insight.

Mol Biol Rep 2018 Dec 8. Epub 2018 Dec 8.

Department of Pharmacognosy & Phytopharmacy, TIFAC CORE in Herbal Drugs, JSS College of Pharmacy (A Constituent College of JSS Academy of Higher Education & Research, Mysuru, Karnataka, India), P.B. 20, Rocklands, Ootacamund, Tamil Nadu, 643001, India.

Mitochondria play a central role in the energy homeostasis in eukaryotic cells by generating ATP via oxidative metabolism of nutrients. Excess lipid accumulation and impairments in mitochondrial function have been considered as putative mechanisms for the pathogenesis of skeletal muscle insulin resistance. Accumulation of lipids in tissues occurs due to either excessive fatty acid uptake, decreased fatty acid utilization or both. Read More

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December 2018

Ginsenoside Compound K Regulates Amyloid β via the Nrf2/Keap1 Signaling Pathway in Mice with Scopolamine Hydrobromide-Induced Memory Impairments.

J Mol Neurosci 2018 Dec 7. Epub 2018 Dec 7.

Laboratory of Molecular Pharmacology, Jilin Provincial Key Laboratory of BioMacromolecules of Chinese Medicine, Jilin Ginseng Academy, Changchun University of Chinese Medicine, Changchun, 130117, Jilin, China.

The objective of this study was to investigate the neuroprotective and antioxidant effects of ginsenoside compound K (CK) in a model of scopolamine hydrobromide-induced, memory-impaired mice. The role of CK in the regulation of amyloid β (Aβ) and its capacity to activate the Nrf2/Keap1 signaling pathway were also studied due to their translational relevance to Alzheimer's disease. The Morris water maze was used to assess spatial memory functions. Read More

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December 2018

Curcumin and quercetin synergistically attenuate subacute diazinon-induced inflammation and oxidative neurohepatic damage, and acetylcholinesterase inhibition in albino rats.

Environ Sci Pollut Res Int 2018 Dec 8. Epub 2018 Dec 8.

Department of Zoology, Science College, King Saud University, Riyadh, Saudi Arabia.

The ubiquitous use of diazinon (DZN, an organophosphorus insecticide) has increased the probability of occupational, public, and the ecosystem exposure; these exposures are linked to negative health outcomes. The flavonoids curcumin (CUR) and quercetin (QUE) exert significant anti-inflammatory and antioxidant activities against toxicants, including insecticides. However, it is unclear whether their combination enhances these activities. Read More

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December 2018

A Rat Eye Lens Model of Cataract Formation.

Authors:
Paul C Guest

Methods Mol Biol 2019 ;1916:311-318

Laboratory of Neuroproteomics, Department of Biochemistry and Tissue Biology, Institute of Biology, University of Campinas (UNICAMP), Campinas, SP, Brazil.

This chapter describes the use of lenses obtained from rats as a model of cataractogenesis. At the molecular level, this is visualized as reduced activity of oxidative reductive enzymes such as aldose reductase and increased proteolysis of lens structural proteins including vimentin. In this chapter, protocols for assessment of these two pathways are presented. Read More

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January 2019

Cryopreservation of Red Blood Cells.

Methods Mol Biol 2019 ;1916:233-238

Department of Chemical Engineering and Biotechnology, University of Cambridge, Cambridge, UK.

Glycerol and trehalose are widely used protective agents in the cryopreservation of red blood cells (RBCs). This chapter presents a protocol for use of these agents as cryoprotectants of RBCs, with post-thaw assessment of cell survival and cellular oxidative-reductive status. The main aim is to provide a framework for further studies aimed at improving RBC survival and function and to supply improved biomaterials for studies on RBC biochemistry, major operations, as well as those for urgent use in emergency room situations. Read More

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January 2019

MiR-15b-5p is Involved in Doxorubicin-Induced Cardiotoxicity via Inhibiting Bmpr1a Signal in H9c2 Cardiomyocyte.

Cardiovasc Toxicol 2018 Dec 7. Epub 2018 Dec 7.

Department of Cardiology, The Second Affiliated Hospital of Shantou University Medical College, Dongxia North Road, Shantou, 515041, Guangdong, People's Republic of China.

The wide use of anthracyclines represented by doxorubicin (DOX) has benefited cancer patients, yet the clinical application is limited due to its cardiotoxicity. Although numerous evidences have supported a role of microRNAs (miRNAs) in DOX-induced myocardial damage, the exact etiology and pathogenesis remain largely obscure. In this study, we focused on the role of miR-15b-5p in DOX-induced cardiotoxicity. Read More

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December 2018

Combined actions of blueberry extract and lithium on neurochemical changes observed in an experimental model of mania: exploiting possible synergistic effects.

Metab Brain Dis 2018 Dec 7. Epub 2018 Dec 7.

Programa de Pós-Graduação em Bioquímica e Bioprospecção, Centro de Ciências Químicas, Farmacêuticas e de Alimentos, Universidade Federal de Pelotas, Pelotas, RS, Brazil.

Bipolar disorder is a psychiatric disease characterized by recurrent episodes of mania and depression. Blueberries contain bioactive compounds with important pharmacological effects such as neuroprotective and antioxidant actions. The aim of this study was to investigate the effects of blueberry extract and/or lithium on oxidative stress, and acetylcholinesterase (AChE) and Na, K-ATPase activity in an experimental ketamine-induced model of mania. Read More

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December 2018

Aging and Hyperglycemia Intensify Dyslipidemia-Induced Oxidative Stress and Inflammation in Rats: Assessment of Restorative Potentials of ALA and EPA + DHA.

Inflammation 2018 Dec 10. Epub 2018 Dec 10.

Department of Biochemistry, CSIR-Central Food Technological Research Institute, Mysore, Karnataka, 570020, India.

Effect of aging and hyperglycemia on oxidative stress (OS) and inflammation in dyslipidemic conditions has not been elucidated. Hence, in this study, we assessed the implications of aging, hyperglycemia, and also the dietary effect of n-3 fatty acids (α-linolenic acid (ALA) and eicosapentaenoic acid (EPA) + docosahexaenoic acid (DHA)) on OS and inflammation in dyslipidemic rats. Dyslipidemia was induced in young and aged rats by feeding high-fat lard (HFL) diet. Read More

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December 2018

Interplay Between Metabolic Sensors and Immune Cell Signaling.

Exp Suppl 2018 ;109:115-196

Trident Academy of Creative Technology, Bhubaneswar, Odisha, India.

The immune system, like all other systems, responds to perturbations of the baseline, homeostatic functioning of immune cells. These perturbations come in the form of infection, tumors, autoantigens, and can occur after mismatched transplantation. During response, immune cells alter their metabolic activities. Read More

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January 2018
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2-Methoxyestradiol attenuates liver fibrosis in mice: implications for M2 macrophages.

Naunyn Schmiedebergs Arch Pharmacol 2018 Dec 7. Epub 2018 Dec 7.

Department of Anatomy and Embryology, Faculty of Veterinary Medicine, Kafrelsheikh University, Kafrelsheikh, Egypt.

Liver fibrosis is a major health problem worldwide due to its serious complications including cirrhosis and liver cancer. 2-Methoxyestradiol (2-ME) is an end metabolite of estradiol with anti-proliferative, antioxidant, and anti-inflammatory activities. However, the protective role of 2-ME in liver fibrosis has not been fully investigated. Read More

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December 2018

The Effects of Probiotic Supplementation on Genetic and Metabolic Profiles in Patients with Gestational Diabetes Mellitus: a Randomized, Double-Blind, Placebo-Controlled Trial.

Probiotics Antimicrob Proteins 2018 Dec 8. Epub 2018 Dec 8.

Research Center for Biochemistry and Nutrition in Metabolic Diseases, Kashan University of Medical Sciences, Kashan, I.R., Iran.

This study was carried out to evaluate the effects of probiotic supplementation on genetic and metabolic profiles in patients with gestational diabetes mellitus (GDM) who were not on oral hypoglycemic agents. This randomized, double-blind, placebo-controlled clinical trial was conducted in 48 patients with GDM. Participants were randomly divided into two groups to intake either probiotic capsule containing Lactobacillus acidophilus, Lactobacillus casei, Bifidobacterium bifidum, Lactobacillus fermentum (2 × 10 CFU/g each) (n = 24) or placebo (n = 24) for 6 weeks. Read More

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December 2018

Ginsenoside Rg1 protects against H2O2‑induced neuronal damage due to inhibition of the NLRP1 inflammasome signalling pathway in hippocampal neurons in vitro.

Int J Mol Med 2018 Nov 30. Epub 2018 Nov 30.

Department of Pharmacology, Key Laboratory of Anti‑inflammatory and Immunopharmacology, Ministry of Education, Anhui Medical University, Hefei, Anhui 230032, P.R. China.

Oxidative stress and neuroinflammation are important in the pathogenesis of ageing and age‑related neurodegenerative diseases, including Alzheimer's disease. NADPH oxidase 2 (NOX2) is a major source of reactive oxygen species (ROS) in the brain. The nucleotide‑binding oligomerisation domain (NOD)‑like receptor protein 1 (NLRP1) inflammasome is responsible for the formation of pro‑inflammatory molecules in neurons. Read More

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November 2018

Glutathione peroxidase 4 maintains a stemness phenotype, oxidative homeostasis and regulates biological processes in Panc‑1 cancer stem‑like cells.

Oncol Rep 2018 Dec 6. Epub 2018 Dec 6.

Chongqing Key Laboratory of Translational Research for Cancer Metastasis and Individualized Treatment, Chongqing University Cancer Hospital and Chongqing Cancer Institute and Chongqing Cancer Hospital, Chongqing 400030, P.R. China.

Reactive oxygen species (ROS) have been widely accepted as critical molecules playing regulatory roles in various biological processes, including proliferation, differentiation and apoptotic/ferroptotic/necrotic cell death. Emerging evidence suggests that ROS may be involved in the induction of epithelial‑to‑mesenchymal transition (EMT), which has been reported to promote cancer stem‑like cell (CSC) generation. Recent data indicate that altered accumulation of ROS is associated with CSC generation, EMT and hypoxia exposure, but the underlying mechanisms are poorly understood. Read More

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December 2018

Chrysin protects human osteoarthritis chondrocytes by inhibiting inflammatory mediator expression via HMGB1 suppression.

Mol Med Rep 2018 Dec 4. Epub 2018 Dec 4.

Translational Research Centre of Regenerative Medicine and 3D Printing Technologies of Guangzhou Medical University, State Key Laboratory of Respiratory Disease, The Third Affiliated Hospital of Guangzhou Medical University, Guangzhou, Guangdong 510150, P.R. China.

High‑mobility group box chromosomal protein (HMGB‑1) contributes to osteoarthritis (OA) by modulating various oxidative, inflammatory and apoptotic signaling pathways. The effect of chrysin (CH), a natural plant flavonoid, and its functional interaction with HMGB‑1, was investigated in a chondrocyte model of OA. Human chondrocytes were pre‑treated with CH, and then subsequently treated with IL‑1β to induce the formation of chondrocytes similar to those found in OA joints. Read More

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December 2018