16,478 results match your criteria Orf


Complete nucleotide sequence of a new potexvirus, 'Cnidium virus X', isolated from Cnidium officinale in Japan.

Arch Virol 2019 Apr 22. Epub 2019 Apr 22.

Faculty of Bioresource Sciences, Akita Prefectural University, Akita, Japan.

A flexuous virus was detected in a Cnidium officinale plant in Japan showing mosaic symptoms. The virus was assigned to the genus Potexvirus based on analysis of its complete nucleotide sequence. The genomic RNA of the virus was 5,964 nucleotides in length, excluding the 3'-terminal poly(A) tail. Read More

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http://dx.doi.org/10.1007/s00705-019-04261-6DOI Listing

Purification and characterization of a novel anti-coagulant from the leech Hirudinaria manillensis.

Zool Res 2019 May 28;40(3):205-210. Epub 2019 Mar 28.

Department of Pharmaceutical Sciences, College of Pharmaceutical Sciences, Soochow University, Suzhou Jiangsu 215123, China.

Protease inhibitors have been reported rarely from the leech . In this study, we purified a novel protease inhibitor (bdellin-HM-2) with anticoagulant properties from . With a molecular weight of 1. Read More

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http://dx.doi.org/10.24272/j.issn.2095-8137.2019.037DOI Listing

Evaluation of selection program by assessing the genetic diversity and inbreeding effects on Nellore sheep growth through pedigree analysis.

Asian-Australas J Anim Sci 2019 Feb 14. Epub 2019 Feb 14.

ICAR - National Dairy Research Institute, Division of Animal Production, Southern Research Station, Bangalore, Karnataka state, India.

Objective: The main objectives of the present study are to assess the genetic diversity, population structure and also to appraise the efficiency of ongoing selective breeding program in the closed nucleus herd of Nellore sheep through pedigree analysis.

Methods: Information utilized in the study was collected from the pedigree records of Livestock Research Station, Palamaner during the period from1989 to 2016. Genealogical parameters like generation interval, pedigree completeness, inbreeding level, average relatedness among the animals and genetic conservation index were estimated based on gene origin probabilities. Read More

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http://dx.doi.org/10.5713/ajas.18.0553DOI Listing
February 2019

Identification and bioactivity of a granulocyte colony-stimulating factor b homologue from large yellow croaker (Larimichthys crocea).

Fish Shellfish Immunol 2019 Apr 19. Epub 2019 Apr 19.

Key Laboratory of Marine Biogenetic Resources, Third Institute of Oceanography, State Oceanic Administration, Xiamen, 361005, China; Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao, 266071, China; Key Laboratory of Marine Biotechnology of Fujian Province, Institute of Oceanology, Fujian Agriculture and Forestry University, Fuzhou, 350002, China. Electronic address:

Granulocyte colony-stimulating factor (GCSF) is a pleiotropic cytokine that plays a key role in regulation of hematopoiesis, innate and adaptive immune responses in mammals. However, bioactivity of GCSF in teleost fish remains largely unknown. In this study, a GCSFb homologue from large yellow croaker (Larimichthys crocea) (LcGCSFb) was cloned by RACE-PCR techniques. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S10504648193028
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http://dx.doi.org/10.1016/j.fsi.2019.04.048DOI Listing
April 2019
3 Reads

A comprehensive reference transcriptome resource for the Iberian ribbed newt Pleurodeles waltl, an emerging model for developmental and regeneration biology.

DNA Res 2019 Apr 22. Epub 2019 Apr 22.

Department of Biomedical Sciences, School of Life Science, Faculty of Medicine, Tottori University, Yonago, Tottori 683-8503, Japan.

Urodele newts have unique biological properties, notably including prominent regeneration ability. The Iberian ribbed newt, Pleurodeles waltl, is a promising model amphibian distinguished by ease of breeding and efficient transgenic and genome editing methods. However, limited genetic information is available for P. Read More

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http://dx.doi.org/10.1093/dnares/dsz003DOI Listing

The RAG2 gene of yellow catfish (Tachysurus fulvidraco) and its immune response against Edwardsiella ictaluri infection.

Dev Comp Immunol 2019 Apr 16. Epub 2019 Apr 16.

Guangzhou Key Laboratory of Aquatic Animal Diseases and Waterfowl Breeding, College of Animal Sciences and Technology, Zhongkai University of Agriculture and Engineering, Guangzhou, Guangdong, 510225, China. Electronic address:

Recombination-activating gene 2 (rag 2) allies with recombination-activating gene 1 (rag 1) and regulates the V(D)J recombination of immunoglobulin (Ig) and T-cell receptor (TCR) genes. Being a key player in the adaptive immune response of vertebrates, functional characterization of rag 2 from yellow catfish is beneficial for understanding the biological response towards the pathogens. In this report, we have cloned and characterized the rag 2 gene of yellow catfish, and a particular pattern of expression was analysed in the major tissues of yellow catfish. Read More

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http://dx.doi.org/10.1016/j.dci.2019.04.006DOI Listing

Transcription attenuation-derived small RNA rnTrpL regulates tryptophan biosynthesis gene expression in trans.

Nucleic Acids Res 2019 Apr 17. Epub 2019 Apr 17.

Institute of Microbiology and Molecular Biology, Justus Liebig University, Giessen, 35392, Germany.

Ribosome-mediated transcription attenuation is a basic posttranscriptional regulation mechanism in bacteria. Liberated attenuator RNAs arising in this process are generally considered nonfunctional. In Sinorhizobium meliloti, the tryptophan (Trp) biosynthesis genes are organized into three operons, trpE(G), ppiD-trpDC-moaC-moeA, and trpFBA-accD-folC, of which only the first one, trpE(G), contains a short ORF (trpL) in the 5'-UTR and is regulated by transcription attenuation. Read More

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http://dx.doi.org/10.1093/nar/gkz274DOI Listing

Phosphate uptake by the phosphonate transport system PhnCDE.

BMC Microbiol 2019 Apr 16;19(1):79. Epub 2019 Apr 16.

Departamento de Microbiologia, Instituto de Ciências Biomédicas Universidade de São Paulo, São Paulo-SP, Brazil.

Background: Phosphate is a fundamental nutrient for all creatures. It is thus not surprising that a single bacterium carries different transport systems for this molecule, each usually operating under different environmental conditions. The phosphonate transport system of E. Read More

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http://dx.doi.org/10.1186/s12866-019-1445-3DOI Listing

Poxviral E3L ortholog (Viral Interferon resistance gene) of orf viruses of sheep and goats indicates species-specific clustering with heterogeneity among parapoxviruses.

Cytokine 2019 Apr 13;120:15-21. Epub 2019 Apr 13.

Division of Virology, ICAR-IVRI, Mukteswar 263 138, Nainital, Uttarakhand, India. Electronic address:

Orf is a contagious disease posing a serious threat to animal and human health. E3L is one of the evolutionarily acquired immunomodulatory proteins present in orf virus (ORFV) and is responsible for conferring resistance to interferons among poxviruses. Genetic analysis of ORFV isolates of different geographical regions including Indian subcontinent targeting viral interferon resistance (VIR) gene (a homolog of vaccinia virus E3L gene) revealed a high percentage of identity among themselves and other ORFV isolates at both nt and aa levels as compared to low identity among parapoxviruses (PPVs). Read More

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http://dx.doi.org/10.1016/j.cyto.2019.04.001DOI Listing
April 2019
2 Reads

Characterisation of scavenger receptor class B type 1 in rare minnow (Gobiocypris rarus).

Fish Shellfish Immunol 2019 Apr 13;89:614-622. Epub 2019 Apr 13.

State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, 430072, China. Electronic address:

Scavenger receptor class B type 1 (SRB1) is a transmembrane protein belonging to the scavenger receptors (SRs) family and it plays an important role in viral entry. Not much is known on SRB1 in teleost fish. Grass carp reovirus (GCRV) cause huge economic losses in grass carp industry. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S10504648193026
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http://dx.doi.org/10.1016/j.fsi.2019.04.031DOI Listing
April 2019
1 Read

The first cloned sea cucumber FADD from Holothuria leucospilota: Molecular characterization, inducible expression and involvement of apoptosis.

Fish Shellfish Immunol 2019 Apr 13;89:548-554. Epub 2019 Apr 13.

CAS Key Laboratory of Tropical Marine Bio-resources and Ecology (LMB), Guangdong Provincial Key Laboratory of Applied Marine Biology (LAMB), South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou, 510301, PR China. Electronic address:

In this study, a sea cucumber Fas-associated death domain (FADD) named HLFADD was first cloned from Holothuria leucospilota. The full-length cDNA of HLFADD is 2137 bp in size, containing a 116-bp 5'-untranslated region (UTR), a 1334-bp 3'-UTR and a 687-bp open reading frame (ORF) encoding a protein of 228 amino acids with a deduced molecular weight of 26.42 kDa. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S10504648193026
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http://dx.doi.org/10.1016/j.fsi.2019.04.030DOI Listing
April 2019
2 Reads

Visible-Light Induced Regioselective Cycloaddition of Benzoyl Azides and Alkenes to Oxazolines.

J Org Chem 2019 Apr 16. Epub 2019 Apr 16.

Visible-light catalysis allows the regioselective synthesis of oxazolines in high yields. The photosensitized reaction permits the intermolecular formation of oxazolines with a wide functional group tolerance on both partners, benzoyl azides and alkenes. Mechanistic investigations suggest the sensitization of the azide moiety as the activation step. Read More

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http://pubs.acs.org/doi/10.1021/acs.joc.9b00568
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http://dx.doi.org/10.1021/acs.joc.9b00568DOI Listing
April 2019
2 Reads

[Cloning and expression analysis of CtbHLH transcription factor gene from Carthamus tinctorius and construction of plant expression vector].

Zhongguo Zhong Yao Za Zhi 2019 Jan;44(2):278-282

College of Life Science,Jilin Agricultural University Changchun 130118,China Bioreactor and Drug Development Reseach Center,Ministry of Education,Jilin Agricultural University Changchun 130118,China.

To clone bHLH( basic helix-loop-helix) gene from Carthamus tinctorius,analyze the expression level in different plant tissues and construct the plant expression vector. The bHLH1 gene was cloned by RT-PCR techniques,and the protein characteristics were analyzed by bioinformatics,and phylogenetic tree was constructed. The expression of bHLH1 gene in different tissues and the roots after inoculated by Fusarium oxysporum were analyzed using real time-PCR,and the plant expression vector p BASTA-bHLH1 was constructed. Read More

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http://dx.doi.org/10.19540/j.cnki.cjcmm.20181106.011DOI Listing
January 2019

[Cloning and expression analysis of LncRNA-RgAGT2 responding to consecutive monoculture in Rehmannia glutinosa].

Zhongguo Zhong Yao Za Zhi 2019 Feb;44(4):703-711

College of Life Sciences,Henan Agricultural University Zhengzhou 450002,China.

The consecutive monoculture obstacle is a major problem in the field of Rehmannia glutinosa( R. glutinosa),has severely declined the yield and quality of R. glutinosa. Read More

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http://dx.doi.org/10.19540/j.cnki.cjcmm.20181204.003DOI Listing
February 2019

Construction and Activity Analyses of Single Functional Mouse Peroxiredoxin 6 (Prdx6).

J Vet Res 2019 Mar 22;63(1):99-105. Epub 2019 Mar 22.

Key Laboratory of Zoonosis Research, Institute of Zoonosis/College of Veterinary Medicine, Jilin University, Xi An Da Lu 5333, Changchun 130062, China.

Introduction: Peroxiredoxin 6 (Prdx6) is a bifunctional protein with glutathione peroxidase activity and phospholipase A2 activity. Previous studies have shown a significant positive correlation between the intracellular survival ability of and Prdx6. Here, the Prdx6 enzyme with a single activity was constructed to facilitate study of the relationship between the single function of Prdx6 and infection. Read More

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https://content.sciendo.com/view/journals/jvetres/63/1/artic
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http://dx.doi.org/10.2478/jvetres-2019-0004DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6458554PMC
March 2019
1 Read

Tomato Twisted Leaf Virus: A Novel Indigenous New World Monopartite Begomovirus Infecting Tomato in Venezuela.

Viruses 2019 Apr 4;11(4). Epub 2019 Apr 4.

UCLouvain, Earth and Life Institute, Applied Microbiology-Phytopathology, Croix du Sud 2-L07.05.03, 1348 Louvain-la-Neuve, Belgium.

Begomoviruses are one of the major groups of plant viruses with an important economic impact on crop production in tropical and subtropical regions. The global spread of its polyphagous vector, the whitefly , has contributed to the emergence and diversification of species within this genus. In this study, we found a putative novel begomovirus infecting tomato plants in Venezuela without a cognate DNA-B component. Read More

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http://dx.doi.org/10.3390/v11040327DOI Listing

A novel picornavirus in feces of a rainbow lorikeet (Trichoglossus moluccanus) shows a close relationship to members of the genus Avihepatovirus.

Arch Virol 2019 Apr 13. Epub 2019 Apr 13.

Department of Microbiology, School of Medicine, Jiangsu University, Zhenjiang, 212013, Jiangsu, China.

A novel picornavirus, named "lorikeet picornavirus 1" (LoPV-1), was detected in a fecal sample from rainbow lorikeets using viral metagenomic analysis, and its complete genome sequence was determined and analyzed. The genome of LoPV-1 is 7862 nt long, including a 617-nt 5' UTR, a type IV IRES 5'UTR with an '8-like' motif, a 7032-nt polyprotein ORF, and a 213-nt 3' UTR. Phylogenetic analysis and pairwise asequence comparisons based on the amino acid sequences of P1, P2, and P3 indicated that LoPV-1 showed the closest relationship to two picornaviruses that were isolated recently from red-crowned cranes and clustered together with members of the genus Avihepatovirus. Read More

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http://dx.doi.org/10.1007/s00705-019-04246-5DOI Listing

Characterization of a CD59 in orange-spotted grouper (Epinephelus coioides).

Fish Shellfish Immunol 2019 Apr 11;89:486-497. Epub 2019 Apr 11.

Guangzhou Key Laboratory of Subtropical Biodiversity and Biomonitoring, Guangdong Provincial Key Laboratory for Healthy and Safe Aquaculture, College of Life Science, South China Normal University, Guangzhou, 510631, PR China. Electronic address:

CD59, a multifunctional glycoprotein, not only plays a regulatory role in complement cascades, but also participates in modulation of teleostean immunity. In this study, full length sequence of EcCD59 was obtained, comprising a 5'UTR of 163 bp, an ORF of 354 bp and a 3'UTR of 559 bp. EcCD59 gene encoded a polypeptide of 117 amino acids. Read More

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http://dx.doi.org/10.1016/j.fsi.2019.04.020DOI Listing

Characterization of the bacteriophage φEf11 ORF 28 endolysin: A multi-functional lytic enzyme with properties distinct from all other identified phage endolysins.

Appl Environ Microbiol 2019 Apr 12. Epub 2019 Apr 12.

Department of Endodontology, Kornberg School of Dentistry, Temple University, Philadelphia, PA, USA

φEf11 is a temperate, bacteriophage that infects strains of The φEf11 genome, encompassing 65 open reading frames (ORFs), is contained within 42,822 bp of DNA. Within this genome, a module of six lysis-related genes was identified. Based upon sequence homology, one of these six genes, ORF 28, was predicted to code for an N- acetylmuramoyl-L-alanine amidase endolysin of 46. Read More

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http://dx.doi.org/10.1128/AEM.00555-19DOI Listing

Molecular characterization and biological functioning of interleukin-8 in Siberian sturgeon (Acipenser baeri).

Fish Shellfish Immunol 2019 Apr 9. Epub 2019 Apr 9.

Beijing Fisheries Research Institute& Beijing Key Laboratory of Fishery Biotechnology, Beijing, 100068, People's Republic of China; National Freshwater Fisheries Engineering Technology Research Center, Beijing, 100068, People's Republic of China. Electronic address:

Interleukin-8, otherwise known as CXCL8, is a CXC chemokine that plays a pivotal regulatory role in immune and inflammation responses of animals. Here, we identified an interleukin-8 homologue from Siberian sturgeon (Acipenser baeri), named AbIL-8, which belongs to the lineage 1 group of teleost fish IL-8s. The cDNA of Abil-8 is 1130 bp in length, containing a 5'- untranslated region (UTR) of 50 bp, a 3'- UTR of 783 bp, and an open reading frame (ORF) of 297 bp that encodes a protein consisting of 98 amino acids. Read More

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http://dx.doi.org/10.1016/j.fsi.2019.04.010DOI Listing
April 2019
1 Read

Functional characterization of dark sleeper (Odontobutis obscura) IRF3 in IFN regulation.

Fish Shellfish Immunol 2019 Apr 10;89:411-419. Epub 2019 Apr 10.

State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, China. Electronic address:

The dark sleeper, Odontobutis obscura (O. obscura), is a commercially important species of freshwater sleeper native to East Asia. However, its molecular biology system is unexplored, including the interferon (IFN) signaling pathway, which is crucial to the antiviral response. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S10504648193025
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http://dx.doi.org/10.1016/j.fsi.2019.04.019DOI Listing
April 2019
1 Read
2.674 Impact Factor

The distribution, expression of the Cu/Zn superoxide dismutase in Apostichopus japonicus and its function for sea cucumber immunity.

Fish Shellfish Immunol 2019 Apr 9. Epub 2019 Apr 9.

Department of Biotechnology, School of Biological Engineering, Dalian Polytechnic University, Dalian, 116034, Liaoning Province, PR China. Electronic address:

Cu/Zn superoxide dismutases (SODs) are antioxidative metalloenzymes that exist ubiquitously in different species and are distributed widely in various tissues and cell types. In this study, the distribution and biological function of the Cu/Zn superoxide dismutase in Apostichopus japonicus (AjSOD1) is first characterized. The AjSOD1 cDNA is 1219 bp in length and contains an open reading frame (ORF) of 459 bp that encodes a protein of 152 amino acids with a deduced molecular weight of 15. Read More

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http://dx.doi.org/10.1016/j.fsi.2019.04.009DOI Listing
April 2019
1 Read

Craniofrontonasal Syndrome Caused by Introduction of a Novel uATG in the 5'UTR of .

Mol Syndromol 2019 Feb 3;10(1-2):40-47. Epub 2018 Jul 3.

Centro de Pesquisa sobre o Genoma Humano e Células-Tronco (CEGH-CEL), Curitiba, Brazil.

Craniofrontonasal syndrome (CFNS) is an X-linked disorder caused by mutations in which females are more severely affected than males. Severe male phenotypes are associated with mosaicism, supporting cellular interference for sex bias in this disease. Although many variants have been found in the coding region of , only 2 pathogenic variants have been identified in the same nucleotide in 5'UTR, disrupting the stop codon of an upstream open reading frame (uORF). Read More

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http://dx.doi.org/10.1159/000490635DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6422142PMC
February 2019
3 Reads

Molecular cloning, characterization of Pomacea canaliculata HSP40 and its expression analysis under temperature change.

J Therm Biol 2019 Apr 2;81:59-65. Epub 2019 Feb 2.

Zhejiang Provincial Key Laboratory of Biometrology and Inspection & Quarantine, College of Life Sciences, China Jiliang University, 258 Xueyuan Street, Xiasha, Hangzhou 310018, China. Electronic address:

Heat shock proteins (HSPs) play important roles in the adaption of Pomacea canaliculata to unsuitable environments. In the present study, a cDNA encoding HSP40 in P. canaliculata (PocaHSP40) was cloned and characterized. Read More

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http://dx.doi.org/10.1016/j.jtherbio.2019.02.006DOI Listing

Characterization of the Chloroplast Genome of (Trentepohliales, Chlorophyta), and Discussion of its Taxonomy.

Int J Mol Sci 2019 Apr 10;20(7). Epub 2019 Apr 10.

Key Laboratory of Algal Biology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China.

Trentepohliales is an aerial order of Chlorophyta with approximately 80 species distributed mainly in tropical and subtropical regions. The taxonomy of this genus is quite difficult and presents a challenge for many phycologists. Although plentiful molecular data is available, most of the sequences are not identified at the species level. Read More

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http://dx.doi.org/10.3390/ijms20071774DOI Listing
April 2019
1 Read

Characterization and functional analysis of a novel mannose-binding lectin from the swimming crab Portunus trituberculatus.

Fish Shellfish Immunol 2019 Apr 8;89:448-457. Epub 2019 Apr 8.

School of Marine Science, Ningbo University, Zhejiang, Ningbo, 315211, China; Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao, 266071, China. Electronic address:

Mannose-binding lectin (MBL) is a pattern recognition receptor (PRR) that plays an important role in the innate immune response. In this study, a novel mannose-binding lectin was cloned from the swimmimg crab Portunus trituberculatus (designated as PtMBL). The complete cDNA of PtMBL gene was 1208 bp in length with an open reading frame (ORF) of 732 bp that encoded 244 amino acid proteins. Read More

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http://dx.doi.org/10.1016/j.fsi.2019.04.007DOI Listing

Macrobrachium rosenbergii Cu/Zn superoxide dismutase (Cu/Zn SOD) expressed in Saccharomyces cerevisiae and evaluation of the immune function to Vibrio parahaemolyticus.

Fish Shellfish Immunol 2019 Apr 8. Epub 2019 Apr 8.

Institute of Agricultural Resources and Environment, Jiangsu Academy of Agricultural Sciences, Nanjing, China; Key Laboratory of Agro-Environment in Downstream of Yangtze Plain, Ministry of Agriculture, Nanjing, China. Electronic address:

Superoxide dismutases (SODs) are important antioxidant enzymes that occur in virtually all oxygen-respiring organisms, and copper/zinc SOD (Cu/ZnSOD) is one of the most important SODs. In the present study, Macrobrachium rosenbergii Cu/Zn-SOD was expressed in a yeast eukaryotic system. The open reading frame (ORF) of MrCu/ZnSOD was cloned into the plasmid vector pHAC181, and the recombinant plasmid was integrated into the downstream region of the GAL1 promoter in Saccharomyces cerevisiae strain GAL1-ScRCH1 via homologous recombination. Read More

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http://dx.doi.org/10.1016/j.fsi.2019.04.016DOI Listing

SpTGase plays an important role in the hemolymph clotting in mud crab (Scylla paramamosain).

Fish Shellfish Immunol 2019 Apr 9;89:326-336. Epub 2019 Apr 9.

Guangdong Provincial Key Laboratory of Marine Biology, Shantou University, Shantou, 515063, China; Marine Biology Institute, Shantou University, Shantou, 515063, China; STU-UMT Joint Shellfish Research Laboratory, Shantou University, Shantou, 515063, China. Electronic address:

Transglutaminase (TGase) is important in blood coagulation, a conserved immunological defense mechanism among invertebrates. This study is the first report of the TGase in mud crab (Scylla paramamosain) (SpTGase) with a 2304 bp ORF encoding 767 amino acids (molecular weight 85.88 kDa). Read More

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http://dx.doi.org/10.1016/j.fsi.2019.04.006DOI Listing
April 2019
2 Reads

Genome sequence analysis of 91 Salmonella Enteritidis isolates from mice caught on poultry farms in the mid 1990s.

Genomics 2019 Apr 8. Epub 2019 Apr 8.

Food and Drug Administration, Center for Food Safety and Applied Nutrition, College Park, MD 20740.

A total of 91 draft genome sequences were used to analyze isolates of Salmonella enterica serovar Enteritidis obtained from feral mice caught on poultry farms in Pennsylvania. One objective was to find mutations disrupting open reading frames (ORFs) and another was to determine if ORF-disruptive mutations were present in isolates obtained from other sources. A total of 83 mice were obtained between 1995-1998. Read More

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http://dx.doi.org/10.1016/j.ygeno.2019.04.005DOI Listing

Mass mortality in Korean bay scallop (Argopecten irradians) associated with Ostreid Herpesvirus-1 μVar.

Transbound Emerg Dis 2019 Apr 10. Epub 2019 Apr 10.

Laboratory of Aquatic Biomedicine, College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University, Seoul, 08826, Republic of Korea.

Since November 2017, mass mortalities of larvae of bay scallop (Argopecten irradians) were reported in hatcheries located at the southern area of Republic of Korea. Over 90% of larvae aged 5 to 10 days sank to the bottom of the tank and died. The hatcheries could not produce spat and thus artificial seed production industry incurred huge losses. Read More

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http://dx.doi.org/10.1111/tbed.13200DOI Listing

Testis-specific calcium-binding protein CBP86-IV (CABYR) binds with phosphoglycerate kinase 2 in vitro and in vivo experiment.

Andrologia 2019 Apr 10:e13287. Epub 2019 Apr 10.

Guangxi Key Laboratory of Utilization of Microbial and Botanical Resources, Guangxi Key Laboratory for Polysaccharide Materials and Modifications, School of Marine Sciences and Biotechnology, Guangxi University for Nationalities, Nanning, China.

The investigation of the interacting proteins with testis-specific calcium-binding protein CBP86-IV (CABYR) was carried out in human spermatozoa. The total RNA from human spermatozoa was extracted, and the ORF sequence of TSCBP86-IV gene was amplified and cloned into expression vector pET-28a. The positive recombinant clones were transformed into Escherichia coli strain BL21 (DE3) to express fusion protein. Read More

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https://onlinelibrary.wiley.com/doi/abs/10.1111/and.13287
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http://dx.doi.org/10.1111/and.13287DOI Listing
April 2019
5 Reads

Molecular cloning and expression analysis of SRLK1 gene in self-incompatible Asteraceae species Erigeron breviscapus.

Mol Biol Rep 2019 Apr 8. Epub 2019 Apr 8.

National and Local Joint Engineering Research Center on Germplasm Utilization & Innovation of Chinese Medicinal Materials in Southwest, Yunnan Agricultural University, Kunming, 650201, Yunnan, China.

Based on the transcriptome data, using RACE techniques, we cloned the full-length EbSRLK1 gene in a medicinal, self-incompatible Asteraceae species, Erigeron breviscapus. Bioinformatics approaches were used to analyze the DNA and protein sequences, physical and chemical properties, and domains of the encoded protein. The full-length EbSRLK1 cDNA is 2891 base pairs (bp) with an open reading frame (ORF) of 2634 bp, which encodes the EbSRLK1 protein with 878 amino acids and an estimated molecular weight of 98. Read More

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http://dx.doi.org/10.1007/s11033-019-04773-zDOI Listing
April 2019
2.024 Impact Factor

Discovery and genetic characterization of diverse smacoviruses in Zambian non-human primates.

Sci Rep 2019 Apr 8;9(1):5045. Epub 2019 Apr 8.

Division of Molecular Pathobiology, Research Center for Zoonosis Control, Hokkaido University, Sapporo, 001-0020, Japan.

The Smacoviridae has recently been classified as a family of small circular single-stranded DNA viruses. An increasing number of smacovirus genomes have been identified exclusively in faecal matter of various vertebrate species and from insect body parts. However, the genetic diversity and host range of smacoviruses remains to be fully elucidated. Read More

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http://dx.doi.org/10.1038/s41598-019-41358-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6453971PMC
April 2019
1 Read

Human Papillomavirus Genome based Detection and Typing: A Holistic Molecular Approach.

Curr Mol Med 2019 Apr 5. Epub 2019 Apr 5.

Department of Medicine, Institute of Medical Sciences, Banaras Hindu University, Varanasi-221005. India.

Human Papillomavirus (HPV) is a species specific double-stranded DNA virus infecting human cutaneous or mucosal tissues. The genome structure of HPV is extremely polymorphic hence making it difficult to discriminate between them. HPV exhibits numerous dissimilar types that can be subdivided into high-risk (HR), probably high-risk and low-risk (LR), causing numerous types of cancers and warts around the genital organs in humans. Read More

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http://dx.doi.org/10.2174/1566524019666190405120441DOI Listing

Extensive Ethnic Variation and Linkage Disequilibrium at the Locus: Different Genetic Associations Revealed in Kawasaki Disease.

Front Immunol 2019 21;10:185. Epub 2019 Mar 21.

Department of Blood Cell Research, Sanquin Research and Landsteiner Laboratory, Amsterdam UMC, University of Amsterdam, Amsterdam, Netherlands.

The human Fc-gamma receptors (FcγRs) link adaptive and innate immunity by binding immunoglobulin G (IgG). All human low-affinity FcγRs are encoded by the locus containing functional single nucleotide polymorphisms (SNPs) and gene copy number variants. This locus is notoriously difficult to genotype and high-throughput methods commonly used focus on only a few SNPs. Read More

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http://dx.doi.org/10.3389/fimmu.2019.00185DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6437109PMC
March 2019
2 Reads

A small intergenic region of lp17 is required for evasion of adaptive immunity and induction of pathology by the Lyme disease spirochete.

Cell Microbiol 2019 Apr 3:e13029. Epub 2019 Apr 3.

Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, Washington, USA.

The causative agent of Lyme disease, Borrelia burgdorferi, harbours a single linear chromosome and upwards of 23 linear and circular plasmids. Only a minority of these plasmids, including linear plasmid 17, are maintained with near-absolute fidelity during extended in vitro passage, and characterisation of any putative virulence determinants they encode has only recently begun. In this work, a mutant lacking a ~4. Read More

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http://dx.doi.org/10.1111/cmi.13029DOI Listing
April 2019
4 Reads

HPV18 Utilizes Two Alternative Branch Sites for E6*I Splicing to Produce E7 Protein.

Virol Sin 2019 Apr 3. Epub 2019 Apr 3.

Tumor Virus RNA Biology Section, RNA Biology Laboratory, Center for Cancer Research, National Cancer Institute, NIH, Frederick, MD, 21702, USA.

Human papillomavirus 18 (HPV18) E6 and E7 oncogenes are transcribed as a single bicistronic E6E7 pre-mRNA. The E6 ORF region in the bicistronic E6E7 pre-mRNA contains an intron. Splicing of this intron disrupts the E6 ORF integrity and produces a spliced E6*I RNA for efficient E7 translation. Read More

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http://dx.doi.org/10.1007/s12250-019-00098-0DOI Listing
April 2019
2 Reads

Synthesis of Tri- and Difluoromethoxylated Compounds via Visible Light Photoredox Catalysis.

Angew Chem Int Ed Engl 2019 Apr 3. Epub 2019 Apr 3.

Stony Brook University, Department of Chemistry, 100 Nicolls Road, State University of New York at Stony Brook, Department of Chemistry, 11794, Stony Brook, UNITED STATES.

The trifluoromethoxy (OCF3) and difluoromethoxy (OCF2H) groups are fluorinated structural motifs that exhibit unique physicochemical characteristics. Incorporation of these substituents into organic molecules is a highly desirable approach used in medicinal chemistry and drug discovery processes to alter the properties of a parent compound. Recently, tri- and difluoromethyl ethers have received increasing attention and several innovative strategies to access these valuable functional groups have been developed. Read More

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http://dx.doi.org/10.1002/anie.201902243DOI Listing

Peptidoglycan hydrolase of an unusual cross-link cleavage specificity contributes to bacterial cell wall synthesis.

Proc Natl Acad Sci U S A 2019 Apr 2;116(16):7825-7830. Epub 2019 Apr 2.

Centre for Cellular and Molecular Biology, Council of Scientific and Industrial Research, Hyderabad, India 500007

Bacteria are surrounded by a protective exoskeleton, peptidoglycan (PG), a cross-linked mesh-like macromolecule consisting of glycan strands interlinked by short peptides. Because PG completely encases the cytoplasmic membrane, cleavage of peptide cross-links is a prerequisite to make space for incorporation of nascent glycan strands for its successful expansion during cell growth. In most bacteria, the peptides consist of l-alanine, d-glutamate, -diaminopimelic acid (mDAP) and d-alanine (d-Ala) with cross-links occurring either between d-Ala and mDAP or two mDAP residues. Read More

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http://dx.doi.org/10.1073/pnas.1816893116DOI Listing
April 2019
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Molecular identification and expression analysis of four Lysin motif (LysM) domain-containing proteins from turbot (Scophthalmus maximus).

Fish Shellfish Immunol 2019 Mar 30;89:271-280. Epub 2019 Mar 30.

Marine Science and Engineering College, Qingdao Agricultural University, Qingdao, 266109, PR China. Electronic address:

Lysin motif (LysM) is involved in chitin, peptidoglycan and other structurally-related oligosaccharides recognition and binding, and it is important for the biological processes of responsing to bacterial and viral infections and pathogen defense. LysM is also a widely spread protein, ranging from prokaryotes to eukaryotes, including bacteria, plants and mammals. However, research of LysM in teleosts especially in marine fish was rarely scarce. Read More

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http://dx.doi.org/10.1016/j.fsi.2019.03.074DOI Listing

Molecular cloning, characterization, and expression level analysis of a marine teleost homolog of catalase from big belly seahorse (Hippocampus abdominalis).

Fish Shellfish Immunol 2019 Mar 29;89:647-659. Epub 2019 Mar 29.

Department of Marine Life Sciences & Fish Vaccine Research Center, Jeju National University, Jeju Self-Governing Province, 63243, Republic of Korea; Marine Science Institute, Jeju National University, Jeju Self-Governing Province, 63333, Republic of Korea. Electronic address:

Organisms possess a cellular antioxidant defense system inclusive of ROS scavengers to maintain the homeostasis of antioxidant levels. Catalase is a major ROS scavenger enzyme that plays a significant role in the antioxidant defense mechanism of organisms by reducing toxic hydrogen peroxide molecules into a nontoxic form of oxygen and water with a high turnover rate. In the present study, we performed molecular and functional characterization of the catalase homolog from Hippocampus abdominalis (HaCat). Read More

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http://dx.doi.org/10.1016/j.fsi.2019.03.064DOI Listing
March 2019
1 Read
2.674 Impact Factor

Biological and molecular characterization of two Anticarsia gemmatalis multiple nucleopolyhedrovirus clones exhibiting contrasting virulence.

J Invertebr Pathol 2019 Mar 28;164:23-31. Epub 2019 Mar 28.

Embrapa Recursos Genéticos e Biotecnologia (Cenargen), Parque Estação Biológica, W5 Norte Final, 70770-917 Brasília, DF, Brazil. Electronic address:

Baculovirus natural populations are known to be genetically heterogeneous and such genotypic diversity could have implications in the performance of biocontrol agents. The Anticarsia gemmatalis nucleopolyhedrovirus (AgMNPV) has been widely used to control the velvetbean caterpillar, Anticarsia gemmatalis, in Brazil. In the present work, morphological and molecular analyses as well as the biological activity of AgMNPV genotypes derived from a Brazilian field isolate (AgMNPV-79) were carried out. Read More

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http://dx.doi.org/10.1016/j.jip.2019.03.008DOI Listing
March 2019
1 Read

A novel phage from periodontal pockets associated with chronic periodontitis.

Virus Genes 2019 Mar 29. Epub 2019 Mar 29.

Department of Preventive Dentistry, Ninth People's Hospital, Shanghai JiaoTong University School of Medicine, 639 Zhizaoju Road, Shanghai, China.

Bacteriophages often constitute the majority of periodontal viral communities, but phages that infect oral bacteria remain uncharacterized. Here, we present the genetic analysis of the genome of a novel siphovirus, named Siphoviridae_29632, which was isolated from a patient with periodontitis using a viral metagenomics-based approach. Among 43 predicted open reading frames (ORFs) in the genome, the viral genes encoding structural proteins were distinct from the counterparts of other viruses, although a distant homology is shared among viral morphogenesis proteins. Read More

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http://dx.doi.org/10.1007/s11262-019-01658-yDOI Listing
March 2019
3 Reads

A novel protein encoded by a circular RNA circPPP1R12A promotes tumor pathogenesis and metastasis of colon cancer via Hippo-YAP signaling.

Mol Cancer 2019 03 29;18(1):47. Epub 2019 Mar 29.

Department of Tumor Biological Treatment, the Third Affiliated Hospital of Soochow University, Changzhou, 213003, People's Republic of China.

Background: It has been well established that circular RNAs (circRNAs) play an important regulatory role during tumor progression. Recent studies have indicated that even though circRNAs generally regulate gene expression through miRNA sponges, they may encode small peptides in tumor pathogenesis. However, it remains largely unexplored whether circRNAs are involved in the tumorigenesis of colon cancer (CC). Read More

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http://dx.doi.org/10.1186/s12943-019-1010-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6440158PMC
March 2019
2 Reads

Relative abundance and molecular evolution of Lake Sinai Virus (Sinaivirus) clades.

Authors:
Robert S Cornman

PeerJ 2019 21;7:e6305. Epub 2019 Mar 21.

U.S. Geological Survey, Fort Collins Science Center, Fort Collins, CO, USA.

Lake Sinai Viruses (Sinaivirus) are commonly detected in honey bees () but no disease phenotypes or fitness consequences have yet been demonstrated. This viral group is genetically diverse, lacks obvious geographic structure, and multiple lineages can co-infect individual bees. While phylogenetic analyses have been performed, the molecular evolution of LSV has not been studied extensively. Read More

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http://dx.doi.org/10.7717/peerj.6305DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6431542PMC
March 2019
1 Read

Cloning and characterization of wnt4a gene in a natural triploid teleost, Qi river crucian carp (Carassius auratus).

Gen Comp Endocrinol 2019 Mar 25. Epub 2019 Mar 25.

Key Laboratory of Freshwater Fish Reproduction and Development (Ministry of Education), Key Laboratory of Aquatic Science of Chongqing, School of Life Science, Southwest University, Chongqing 400715, PR China. Electronic address:

WNT4 (wingless-type MMTV integration site family, member 4) plays a key role in the ovarian differentiation and development in mammals. However, the possible roles of Wnt4 during gonadal differentiation and development need further clarification in teleosts. In this study, we cloned and characterized the full-length cDNA of Qi river crucian carp (Carassius auratus) wnt4a gene (CA-wnt4a). Read More

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http://dx.doi.org/10.1016/j.ygcen.2019.03.016DOI Listing
March 2019
3 Reads

Molecular cloning of ESR1, BMPR1B, and FOXL2 and differential expressions depend on maternal age and size during breeding season in cultured Asian yellow pond turtle (Mauremys mutica).

Comp Biochem Physiol B Biochem Mol Biol 2019 Jun 25;232:108-120. Epub 2019 Mar 25.

College of Life Science, Nanchang University, Nanchang, Jiangxi 330031, PR China; Key Laboratory of Tropical & Subtropical Fishery Resource Application & Cultivation, Ministry of Agriculture and Rural Affairs, Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou, Guangdong 510380, PR China. Electronic address:

The reproductive capacity (egg size and egg number) of most of oviparous animals, including the Asian yellow pond turtle (Mauremys mutica), is constrained by the maternal age and body size, but the mechanism determining the maternal reproductive ability remains unclear. To disclose how maternal age and size affect reproductive ability of M. mutica, we first identified the full-length cDNAs from estrogen receptor 1 (ESR1), bone morphogenetic protein receptor 1B (BMPR1B), and forkhead box L2 (FOXL2). Read More

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http://dx.doi.org/10.1016/j.cbpb.2019.03.009DOI Listing
June 2019
1 Read
1.551 Impact Factor

Transducin β-like 1 X-linked receptor 1 (TBLR1) affects RGNNV infection through negative regulation of interferon immune response in orange-spotted grouper, Epinephelus coioides.

Fish Shellfish Immunol 2019 Mar 24;89:76-82. Epub 2019 Mar 24.

College of Marine Sciences, South China Agricultural University, Guangzhou, 510642, China; Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao, 266000, China. Electronic address:

Transducin β-like 1 X-linked receptor 1 (TBLR1) was identified as an important component of nuclear receptor corepressor (N-CoR) complex, and functionally participated in regulation of transcriptional activation. However, the potential roles of TBLR1 in innate immune response still remain uncertain. In the present work, a novel TBLR1 from orange-spotted grouper, Epinephelus coioides (named as EcTBLR1) was cloned and its effect on fish virus infection was characterized. Read More

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http://dx.doi.org/10.1016/j.fsi.2019.03.046DOI Listing
March 2019
1 Read

Aberrant FGFR signaling mediates resistance to CDK4/6 inhibitors in ER+ breast cancer.

Nat Commun 2019 03 26;10(1):1373. Epub 2019 Mar 26.

Departments of Medicine, Vanderbilt University Medical Center, Nashville, 37232-6307, TN, USA.

Using an ORF kinome screen in MCF-7 cells treated with the CDK4/6 inhibitor ribociclib plus fulvestrant, we identified FGFR1 as a mechanism of drug resistance. FGFR1-amplified/ER+ breast cancer cells and MCF-7 cells transduced with FGFR1 were resistant to fulvestrant ± ribociclib or palbociclib. This resistance was abrogated by treatment with the FGFR tyrosine kinase inhibitor (TKI) lucitanib. Read More

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http://www.nature.com/articles/s41467-019-09068-2
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http://dx.doi.org/10.1038/s41467-019-09068-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6435685PMC
March 2019
14 Reads

Plastome reduction and gene content in New World Pilostyles (Apodanthaceae) unveils high similarities to African and Australian congeners.

Mol Phylogenet Evol 2019 Mar 23;135:193-202. Epub 2019 Mar 23.

Instituto de Biología, Universidad de Antioquia UdeA, Medellín, Colombia. Electronic address:

Holoparasitism has led to extreme plastome reduction. Plastomes in the legume holoparasite Pilostyles (Apodanthaceae) are the most reduced in both size and gene content known so far in Embryophytes. Here, we found that the Pilostyles boyacensis plastome, the only American species sequenced so far, is reduced to seven functional genes, accD, rpl2, rrn16 (=16S), rrn23 (=23S), rps3, rps12 and a putative oxidoreductase (PbOx). Read More

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http://dx.doi.org/10.1016/j.ympev.2019.03.014DOI Listing
March 2019
1 Read