16,355 results match your criteria Orf


Identification, expression analysis, and functional characterization of motilin and its receptor in spotted sea bass (Lateolabrax maculatus).

Gen Comp Endocrinol 2019 Feb 13. Epub 2019 Feb 13.

Key Laboratory of Mariculture of Ministry of Education, Ocean University of China, Qingdao 266000, China. Electronic address:

Motilin (MLN), an interdigestive hormone secreted by endocrine cells of the intestinal mucosa, binds to a G protein-coupled receptor to exert its biological function of regulating gastrointestinal motility. In the present study, we identified the prepromotilin and mln receptor (mlnr) from the spotted sea bass, Lateolabrax maculatus. Mln consisted of an ORF of 336 nucleotides encoding 111 amino acids. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S00166480183047
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http://dx.doi.org/10.1016/j.ygcen.2019.02.013DOI Listing
February 2019
2 Reads

Molecular characterisation and transcriptional regulation of muscle growth regulatory factors myogenin and myogenic factor 6 in the trans-Himalayan cyprinid fish Schizothorax richardsonii.

Comp Biochem Physiol A Mol Integr Physiol 2019 Feb 12. Epub 2019 Feb 12.

ICAR-Directorate of Coldwater Fisheries Research, Bhimtal, Uttarakhand 263136, India.

In vertebrates, myogenic regulatory factors (MRFs) play an important role in muscle growth through the processes of cell determination and differentiation. Herein, we report the molecular characterisation of two MRFs, myogenin (myog) and myogenic factor 6 (myf6) in Indian snow trout Schizothorax richardsonii. The full length mRNA sequence of Srmyog and Srmyf6 was 1437 and 1296 bp, with an ORF of 762 and 720 bp, encoding a putative protein of 253 and 239 amino acids, respectively. Read More

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http://dx.doi.org/10.1016/j.cbpa.2019.02.007DOI Listing
February 2019

Molecular mechanism and history of non-sense to sense evolution of antifreeze glycoprotein gene in northern gadids.

Proc Natl Acad Sci U S A 2019 Feb 14. Epub 2019 Feb 14.

School of Integrative Biology, University of Illinois at Urbana-Champaign, Urbana, IL 61801

A fundamental question in evolutionary biology is how genetic novelty arises. De novo gene birth is a recently recognized mechanism, but the evolutionary process and function of putative de novo genes remain largely obscure. With a clear life-saving function, the diverse antifreeze proteins of polar fishes are exemplary adaptive innovations and models for investigating new gene evolution. Read More

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http://dx.doi.org/10.1073/pnas.1817138116DOI Listing
February 2019

[Cloning and expression characteristics of tryptophan hydroxylase (TRH) from silkworm, Bombyx mori].

Sheng Wu Gong Cheng Xue Bao 2019 Jan;35(1):102-113

College of Biotechnology, Southwest University, Chongqing 400715, China.

The biogenic monoamine 5-hydroxytryptamine (5-HT) is an ancient intracellular signaling molecule widely distributed in all animals with nervous systems, and has been implicated in principal behaviors. Tryptophan hydroxylase (TRH) induces a highly specific catalytic reaction that converts L-tryptophan (tryptophan) to 5-hydroxy-L-tryptophan (5-HTP) that is subsequently used as a substrate by aromatic L-amino acid decarboxylase (DDC) to form 5-HT. Five-HT is an ancient intracellular signaling molecule that is widely distributed in the animal kingdom and has been implicated in regulating the behaviors of animals with nervous systems. Read More

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http://dx.doi.org/10.13345/j.cjb.180133DOI Listing
January 2019

Complete nucleotide sequence of a novel mycovirus from Trichoderma harzianum in China.

Arch Virol 2019 Feb 12. Epub 2019 Feb 12.

Institute of Plant Protection, Chinese Academy of Agricultural Sciences, No. 2 West Yuanmingyuan Rd., Haidian District, Beijing, 100193, China.

A new mycovirus was identified in Trichoderma harzianum strain 137 isolated in Xinjiang province, China. The whole genome sequence of the mycovirus was determined by metagenomic sequencing, RT-PCR, and RACE cloning. The mycovirus contained two genomic segments. Read More

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http://dx.doi.org/10.1007/s00705-019-04145-9DOI Listing
February 2019

Simvastatin and other inhibitors of the enzyme 3-hydroxy-3-methylglutaryl coenzyme A reductase of Ustilago maydis (Um-Hmgr) affect the viability of the fungus, its synthesis of sterols and mating.

Rev Iberoam Micol 2019 Feb 7. Epub 2019 Feb 7.

Departamento de Microbiología, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Prol. Carpio y Plan de Ayala S/N, 11340 Ciudad de México, Mexico. Electronic address:

Background: The enzyme 3-hydroxy-3-methylglutaryl coenzyme A reductase (Hmgr) catalyzes the synthesis of mevalonate, a key compound for the synthesis of cholesterol in humans and ergosterol in fungi. Since the Hmgr enzymes of Saccharomyces cerevisiae, Schizosaccharomyces pombe and Candida glabrata are similar to the Hmgr enzymes of mammals, fungal Hmgr enzymes have been proposed as a model for studying antifungal agents.

Aims: To examine the correlation between inhibiting Um-Hmgr enzyme and the viability, sterols synthesis and mating in Ustilago maydis. Read More

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http://dx.doi.org/10.1016/j.riam.2018.05.004DOI Listing
February 2019

Identification and functional analysis of a cytochrome P450 gene involved in imidacloprid resistance in Bradysia odoriphaga Yang et Zhang.

Pestic Biochem Physiol 2019 Jan 17;153:129-135. Epub 2018 Nov 17.

Department of Entomology, China Agricultural University, Beijing 100193, China.

Insect cytochrome P450 monooxygenases played an important role in detoxifying insecticides which potentially contributed to the metabolic resistance to insecticides. Bradysia odoriphaga, as a major pest of Chinese chive, was reported to be highly tolerant to neonicotinoid insecticides imidacloprid. In this study, a novel P450 gene, CYP6FV12, was cloned from B. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S00483575183029
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http://dx.doi.org/10.1016/j.pestbp.2018.11.009DOI Listing
January 2019
2 Reads

mRNA dynamics and alternative conformations adopted under low and high arginine concentrations control polyamine biosynthesis in Salmonella.

PLoS Genet 2019 Feb 11;15(2):e1007646. Epub 2019 Feb 11.

Department of Microbiology and Molecular Genetics, IMRIC, The Hebrew University-Hadassah Medical School, Jerusalem, Israel.

Putrescine belongs to the large group of polyamines, an essential class of metabolites that exists throughout all kingdoms of life. The Salmonella speF gene encodes an inducible ornithine decarboxylase that produces putrescine from ornithine. Putrescine can be also synthesized from arginine in a parallel metabolic pathway. Read More

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http://dx.doi.org/10.1371/journal.pgen.1007646DOI Listing
February 2019

Involvement of a novel protein kinase C (nPKC) in immunocompetence in hemocytes of white shrimp, Litopenaeus vannamei.

Fish Shellfish Immunol 2019 Feb 8;87:590-599. Epub 2019 Feb 8.

Department of Aquaculture, National Pingtung University of Science and Technology, Pingtung, 91201, Taiwan, ROC. Electronic address:

Complementary (c)DNA encoding novel protein kinase C (PKC) messenger (m)RNA of the white shrimp Litopenaeus vannamei, consisted of 2454-bp cDNA containing an open reading frame (ORF) of 2232 bp, belonging to the novel (n)PKC family of proteins characterized by their containing two phorbol ester/diacylglycerol-binding domains (C1 domain), a C2 domain, and a catalytic domain of the serine/threonine kinase, designated LvnPKC. A comparison of amino acid sequences showed that LvnPKC was closely related to arthropod nPKC. LvnPKC cDNA was detected in all tested tissues with a real-time PCR including the hepatopancreas, gills, muscles, subcuticular epithelium, abdominal nerve, thoracic nerve, brain, the stomach, heart, and especially in hemocytes and the intestines. Read More

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http://dx.doi.org/10.1016/j.fsi.2019.02.005DOI Listing
February 2019
1 Read

Concerted expression of a cell cycle regulator and a metabolic enzyme from a bicistronic transcript in plants.

Nat Plants 2019 Feb 8;5(2):184-193. Epub 2019 Feb 8.

Structural Plant Biology Laboratory, Department of Botany and Plant Biology, University of Geneva, Geneva, Switzerland.

Eukaryotic mRNAs frequently contain upstream open reading frames (uORFs), encoding small peptides that may control translation of the main ORF (mORF). Here, we report the characterization of a distinct bicistronic transcript in Arabidopsis. We analysed loss-of-function phenotypes of the inorganic polyphosphatase TRIPHOSPHATE TUNNEL METALLOENZYME 3 (AtTTM3), and found that catalytically inactive versions of the enzyme could fully complement embryo and growth-related phenotypes. Read More

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http://www.nature.com/articles/s41477-019-0358-3
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http://dx.doi.org/10.1038/s41477-019-0358-3DOI Listing
February 2019
1 Read

The complete genome sequence of an alphabaculovirus from Spodoptera exempta, an agricultural pest of major economic significance in Africa.

PLoS One 2019 8;14(2):e0209937. Epub 2019 Feb 8.

Department of Biological Sciences, Simon Fraser University, University Boulevard, Burnaby, British Columbia, Canada.

Spodoptera exempta nucleopolyhedrovirus (SpexNPV) is a viral pathogen of the African armyworm, Spodoptera exempta (Lepidoptera: Noctuidae), a significant agricultural pest of cereal crops in Africa. SpexNPV has been evaluated as a potential insecticide for control of this pest and has served as the subject of research on baculovirus pathology and transmission. Occlusion bodies (OBs) of SpexNPV isolate 244. Read More

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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0209937PLOS
February 2019
1 Read

Assessment of resistance to rice tungro disease in popular rice varieties in India by introgression of a transgene against Rice tungro bacilliform virus.

Arch Virol 2019 Feb 8. Epub 2019 Feb 8.

Department of Plant Molecular Biology, University of Delhi South Campus, New Delhi, 110021, India.

Rice crops in South and Southeast Asian countries suffer critical yield losses due to rice tungro disease caused by joint infection with rice tungro bacilliform virus (RTBV) and rice tungro spherical virus (RTSV). Previously, for generating RNA interference-based transgenic resistance against tungro viruses, RTBV ORF IV was used as a transgene to develop RTBV resistance in a popular high-yielding scented rice variety. The transgene from this line was then introgressed into five popular high-yielding but tungro-susceptible rice varieties by marker-assisted backcross breeding with a view to combine the resistant trait with the agronomic traits. Read More

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http://dx.doi.org/10.1007/s00705-019-04159-3DOI Listing
February 2019

The draft genome sequence of sp. strain CT7, an isolate capable of producing butanol but not acetone and 1,3-propanediol from crude glycerol.

3 Biotech 2019 Feb 1;9(2):63. Epub 2019 Feb 1.

1State Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Puzhu South Road 30#, Nanjing, 211800 People's Republic of China.

A solventogenic sp. strain CT7 which could utilize glycerol directly to produce high yields of butanol was isolated. In the presence of crude glycerol, strain CT7 synthesized butanol through a unique butanol-ethanol (BE) fermentation pathway in which acetone and 1,3-propanediol (1,3-PDO) were not produced. Read More

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http://link.springer.com/10.1007/s13205-019-1598-7
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http://dx.doi.org/10.1007/s13205-019-1598-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6361031PMC
February 2019
1 Read

Cloning, Sequencing and In Silico Analysis of Gene from spp.

Indian J Microbiol 2019 Mar 12;59(1):58-63. Epub 2018 Nov 12.

1Department of Molecular Biology, Biotechnology and Bioinformatics, CCS Haryana Agricultural University, Hisar, India.

We report here isolation and analysis of PCR amplified C gene from spp. strain phbmbb15-B3. This strain was previously developed from mutations of landfill isolates and found to be an efficient Poly Hydroxy butyrate (PHB) producer. Read More

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http://link.springer.com/10.1007/s12088-018-0767-4
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http://dx.doi.org/10.1007/s12088-018-0767-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6328404PMC
March 2019
2 Reads

Contraceptive Sperm Agglutinating Proteins Identified in , Natural Microflora of an Infertile Woman.

Indian J Microbiol 2019 Mar 8;59(1):51-57. Epub 2018 Nov 8.

1Department of Microbiology, South Campus, Basic Medical Science (Block I), Panjab University, Sector 25, Chandigarh, 160014 India.

, isolated from the cervix of an adult female with unexplained infertility, was found to agglutinate human spermatozoa in vitro leading to their death. A genomic library of was generated using pSMART- vector-host system. Approximately 3500 transformants were screened and four showed sperm agglutinating activity. Read More

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http://dx.doi.org/10.1007/s12088-018-0766-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6328407PMC
March 2019
1 Read

RNA editing analysis of ATP synthase genes in the cotton cytoplasmic male sterile line H276A.

Biol Res 2019 Feb 6;52(1). Epub 2019 Feb 6.

Key Laboratory of Plant Genetic and Breeding, College of Agriculture, Guangxi University, Nanning, 530005, People's Republic of China.

Background: Pollen development is an energy-consuming process that particularly occurs during meiosis. Low levels of adenosine triphosphate (ATP) may cause cell death, resulting in CMS (cytoplasmic male sterility). DNA sequence differences in ATP synthase genes have been revealed between the N- and S-cytoplasms in the cotton CMS system. Read More

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https://biolres.biomedcentral.com/articles/10.1186/s40659-01
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http://dx.doi.org/10.1186/s40659-019-0212-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6364438PMC
February 2019
2 Reads

MiRNA-891a-5p mediates HIV-1 Tat and KSHV Orf-K1 synergistic induction of angiogenesis by activating NF-κB signaling.

Nucleic Acids Res 2019 Feb 6. Epub 2019 Feb 6.

State Key Laboratory of Reproductive Medicine, Nanjing Medical University, Nanjing, P.R. China.

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http://dx.doi.org/10.1093/nar/gkz088DOI Listing
February 2019
3 Reads

A Hidden ORF Reveals an Immune Protector.

Biochemistry 2019 Feb 6. Epub 2019 Feb 6.

Clayton Foundation Laboratories for Peptide Biology , Salk Institute for Biological Studies , 10010 North Torrey Pines Road , La Jolla , California 92037-1002 , United States.

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http://dx.doi.org/10.1021/acs.biochem.8b01317DOI Listing
February 2019
1 Read

Evidence for an Integrated Gene Repression Mechanism Based on mRNA Isoform Toggling in Human Cells.

G3 (Bethesda) 2019 Feb 5. Epub 2019 Feb 5.

University of California, Berkeley.

We recently described an unconventional mode of gene regulation in budding yeast by which transcriptional and translational interference collaborate to down-regulate protein expression. Developmentally timed transcriptional interference inhibited production of a well translated mRNA isoform and resulted in the production of an mRNA isoform containing inhibitory upstream open reading frames (uORFs) that prevented translation of the main ORF. Transcriptional interference and uORF-based translational repression are established mechanisms outside of yeast, but whether this type of regulation was conserved was unknown. Read More

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http://dx.doi.org/10.1534/g3.118.200802DOI Listing
February 2019

Magnesium-sensitive upstream ORF controls PRL phosphatase expression to mediate energy metabolism.

Proc Natl Acad Sci U S A 2019 Feb 4. Epub 2019 Feb 4.

Rosalind and Morris Goodman Cancer Research Centre, McGill University, Montréal, QC, Canada H3A 1A3;

Phosphatases of regenerating liver (PRL-1, PRL-2, and PRL-3, also known as PTP4A1, PTP4A2, and PTP4A3) control magnesium homeostasis through an association with the CNNM magnesium transport regulators. Although high PRL levels have been linked to cancer progression, regulation of their expression is poorly understood. Here we show that modulating intracellular magnesium levels correlates with a rapid change of PRL expression by a mechanism involving its 5'UTR mRNA region. Read More

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http://dx.doi.org/10.1073/pnas.1815361116DOI Listing
February 2019
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Computational discovery and annotation of conserved small open reading frames in fungal genomes.

BMC Bioinformatics 2019 Feb 4;19(Suppl 13):551. Epub 2019 Feb 4.

Centre for Frontier Sciences, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, UKM, 43600, Bangi, Selangor, Malaysia.

Background: Small open reading frames (smORF/sORFs) that encode short protein sequences are often overlooked during the standard gene prediction process thus leading to many sORFs being left undiscovered and/or misannotated. For many genomes, a second round of sORF targeted gene prediction can complement the existing annotation. In this study, we specifically targeted the identification of ORFs encoding for 80 amino acid residues or less from 31 fungal genomes. Read More

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http://dx.doi.org/10.1186/s12859-018-2550-2DOI Listing
February 2019

Pomalidomide increases immune surface marker expression and immune recognition of oncovirus-infected cells.

Oncoimmunology 2019 5;8(2):e1546544. Epub 2018 Dec 5.

HIV & AIDS Malignancy Branch, Center for Cancer Research, National Cancer Institute, Bethesda, MD, USA.

Most chronic viruses evade T-cell and natural killer (NK) immunity through downregulation of immune surface markers. Previously we showed that Pomalidomide (Pom) increases surface expression of major histocompatibility complex class I (MHC-I) in Kaposi sarcoma-associated herpesvirus-infected latent and lytic cells and restores ICAM-1 and B7-2 in latent cells. We explored the ability of Pom to increase immune surface marker expression in cells infected by other chronic viruses, including human T-cell leukemia virus type-1 (HTLV-1), Epstein-Barr virus (EBV), human papilloma virus (HPV), Merkel cell polyoma virus (MCV), and human immunodeficiency virus type-1 (HIV-1). Read More

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https://www.tandfonline.com/doi/full/10.1080/2162402X.2018.1
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http://dx.doi.org/10.1080/2162402X.2018.1546544DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6343774PMC
December 2018
2 Reads

Unique gene Pmhyp controlling melanization of pycnidia in Phoma medicaginis.

Fungal Genet Biol 2019 Jan 30;125:53-59. Epub 2019 Jan 30.

Department of Entomology and Plant Pathology, Oklahoma State University, Stillwater, OK, USA.

Phoma medicaginis (syn. Ascochyta medicaginicola Qchen & L. Cai) causes spring black stem and leaf spot of alfalfa and the model legume Medicago truncatula. Read More

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http://dx.doi.org/10.1016/j.fgb.2019.01.007DOI Listing
January 2019

Genomic Characterization of Strain D1701-V () and Development of Novel Sites for Multiple Transgene Expression.

Viruses 2019 Jan 30;11(2). Epub 2019 Jan 30.

Interfaculty Institute for Cell Biology, Department of Immunology, Auf der Morgenstelle 15, 72076 Tübingen, Germany.

The (ORFV; ) strain D1701 with an attenuated phenotype and excellent immunogenic capacity is successfully used for the generation of recombinant vaccines against different viral infections. Adaption for growth in Vero cells was accompanied by additional major genomic changes resulting in ORFV strain variant D1701-V. In this study, restriction enzyme mapping, blot hybridization and DNA sequencing of the deleted region s (A, AT and D) in comparison to the predecessor strain D1701-B revealed the loss of 7 open reading frames (ORF008, ORF101, ORF102, ORF114, ORF115, ORF116, ORF117). Read More

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http://dx.doi.org/10.3390/v11020127DOI Listing
January 2019
1 Read

Naturally Occurring Hepatitis B Virus Mutations Leading to Endoplasmic Reticulum Stress and Their Contribution to the Progression of Hepatocellular Carcinoma.

Int J Mol Sci 2019 Jan 30;20(3). Epub 2019 Jan 30.

Department of Microbiology and Immunology, Biomedical Sciences, Liver Research Institute and Cancer Research Institute, College of Medicine, Seoul National University, Seoul 03080, Korea.

Hepatitis B virus (HBV) infection is a global health problem that causes a wide range of pathological outcomes, including cirrhosis and hepatocellular carcinoma (HCC). Endoplasmic reticulum (ER) stress induction by HBV infection has been implicated in liver carcinogenesis and disease progression with chronic inflammation via enhanced inflammation, oxidative stress-mediated DNA damage, and hepatocyte proliferation. In the natural course of HBV infection, the accumulation of naturally occurring mutations in the HBV genome can generate several mutant types of HBV-encoded proteins, including three different proteins in the S ORF (SHBs, MHBs, and LHBs) and HBcAg in the C ORF, which could contribute to enhanced ER stress in infected hepatocytes mainly via increased ER accumulation of mutant proteins. Read More

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http://dx.doi.org/10.3390/ijms20030597DOI Listing
January 2019

Development of a multiplex TaqMan qPCR assay for simultaneous detection and differentiation of four DNA and RNA viruses from clinical samples of sheep and goats.

J Virol Methods 2019 Jan 28;266:58-64. Epub 2019 Jan 28.

College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi, 712100, China. Electronic address:

Mixed infections with different pathogens are common in sheep and goats under intensive production conditions. Quick and accurate detection and differentiation of different pathogens is necessary for epidemiological surveillance, disease management and import and export controls. Multiplex TaqMan qPCR protocols were developed and subsequently evaluated as effective tools in simultaneously detecting single and mixed infections in sheep and goats. Read More

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http://dx.doi.org/10.1016/j.jviromet.2019.01.015DOI Listing
January 2019
1.883 Impact Factor

The AC4 protein of a cassava geminivirus is required for virus infection.

Mol Plant Microbe Interact 2019 Jan 30. Epub 2019 Jan 30.

Delaware State University, Biological Sciences , 1200 North DuPont HWY , Dover, Delaware, United States , 19901 ;

Geminiviruses (family Geminiviridae) are among the most devastating plant viruses worldwide, causing severe damage in crops of economic and subsistence importance. These viruses have very compact genomes and many of the encoded proteins are multi-functional. Here, we investigated the role of the East African cassava mosaic Cameroon virus (EACMCV) AC4 on virus infectivity in Nicotiana benthamiana. Read More

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https://apsjournals.apsnet.org/doi/10.1094/MPMI-12-18-0354-R
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http://dx.doi.org/10.1094/MPMI-12-18-0354-RDOI Listing
January 2019
12 Reads

Orf virus (ORFV) infection in a three-dimensional human skin model: Characteristic cellular alterations and interference with keratinocyte differentiation.

PLoS One 2019 30;14(1):e0210504. Epub 2019 Jan 30.

Institute of Immunology/Molecular Pathogenesis, Center for Biotechnology and Biomedicine, College of Veterinary Medicine, University of Leipzig, Leipzig, Germany.

ORF virus (ORFV) is the causative agent of contagious ecthyma, a pustular dermatitis of small ruminants and humans. Even though the development of lesions caused by ORFV was extensively studied in animals, only limited knowledge exists about the lesion development in human skin. The aim of the present study was to evaluate a three-dimensional (3D) organotypic culture (OTC) as a human skin model for ORFV infection considering lesion development, replication of the virus, viral gene transcription and modulation of differentiation of human keratinocytes by ORFV. Read More

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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0210504PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6353139PMC
January 2019
2 Reads

Genome-wide analysis of circular RNAs in goat skin fibroblast cells in response to Orf virus infection.

PeerJ 2019 22;7:e6267. Epub 2019 Jan 22.

College of Animal Science and Technology, College of Tropical Agriculture and Forestry, Hainan University, Hainan Key Lab of Tropical Animal Reproduction & Breeding and Epidemic Disease Research, Hainan University, Haikou, China.

Orf, caused by Orf virus (ORFV), is a globally distributed zoonotic disease responsible for serious economic losses in the agricultural sector. However, the mechanism underlying ORFV infection remains largely unknown. Circular RNAs (circRNAs), a novel type of endogenous non-coding RNAs, play important roles in various pathological processes but their involvement in ORFV infection and host response is unclear. Read More

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http://dx.doi.org/10.7717/peerj.6267DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6346991PMC
January 2019

MrSVP, a secreted virulence-associated protein, contributes to thermotolerance and virulence of the entomopathogenic fungus Metarhizium robertsii.

BMC Microbiol 2019 Jan 28;19(1):25. Epub 2019 Jan 28.

Anhui Provincial Key Laboratory of Microbial Pest Control, Anhui Agricultural University, Hefei, 230036, China.

Background: Metarhizium robertsii, a widely distributed insect pathogen, is presently used as a natural alternative to chemical insecticides. Unfortunately, its worldwide commercial use has been restricted by a short shelf life and inconsistencies in virulence. In our previous study, a gene (GenBank accession number EFZ01626) was found to be significantly upregulated in heat-treated conidia. Read More

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http://dx.doi.org/10.1186/s12866-019-1396-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6350332PMC
January 2019

Pharmacological characterization of the 5-HT1A receptor of Bombyx mori and its role in locomotion.

Comp Biochem Physiol A Mol Integr Physiol 2019 Jan 25;231:56-65. Epub 2019 Jan 25.

State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400715, China; College of Biotechnology, Southwest University, Chongqing 400715, China. Electronic address:

Serotonin is involved in the regulation of many physiological and behavioral processes in vertebrates and invertebrates. The effects of serotonin are mediated through interactions of several 5-HT receptor types. The expression and pharmacological properties of 5-HT1 have received more attention than other serotonin receptors, but its functions at the individual level are little studied in arthropods. Read More

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http://dx.doi.org/10.1016/j.cbpa.2019.01.020DOI Listing
January 2019
3 Reads

The copper-inducible copAB operon in Xanthomonas citri subsp. citri is regulated at transcriptional and translational levels.

Microbiology 2019 Jan 28. Epub 2019 Jan 28.

1​Departamento de Bioquímica e Tecnologia Química, Instituto de Química, UNESP, Universidade Estadual Paulista, 14800-900, Araraquara, SP, Brazil.

Upstream open reading frames (ORFs) are frequently found in the 5'-flanking regions of genes and may have a regulatory role in gene expression. A small ORF (named cohL here) was identified upstream from the copAB copper operon in Xanthomonascitri subsp. citri (Xac). Read More

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http://dx.doi.org/10.1099/mic.0.000767DOI Listing
January 2019

Identification of a novel membrane-associated protein from the S7 segment of grass carp reovirus.

J Gen Virol 2019 Jan 28. Epub 2019 Jan 28.

1​National Pathogen Collection Center for Aquatic Animals, Shanghai Ocean University, Shanghai, PR China.

Aquareovirus is a genus of viruses in family Reoviridae, subfamily Spinareovirinae, members of which infect fish, shellfish and crustaceans. Grass carp reovirus (GCRV), a genotype 1 reovirus isolated from grass carp, has served as a model strain for investigating aquareovirus-host interactions. Herein, we report a neglected open reading frame (ORF), tentatively named NS12, residing between NS16 and NS31 in segment 7 (S7) of the virus genome. Read More

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http://dx.doi.org/10.1099/jgv.0.001223DOI Listing
January 2019

Methodologies for Improving HDR Efficiency.

Front Genet 2018 7;9:691. Epub 2019 Jan 7.

College of Veterinary Medicine, Northwest A&F University, Xianyang, China.

Clustered regularly interspaced short palindromic repeats (CRISPR)-associated protein 9 (Cas9) is a precise genome manipulating technology that can be programmed to induce double-strand break (DSB) in the genome wherever needed. After nuclease cleavage, DSBs can be repaired by non-homologous end joining (NHEJ) or homology-directed repair (HDR) pathway. For producing targeted gene knock-in or other specific mutations, DSBs should be repaired by the HDR pathway. Read More

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http://dx.doi.org/10.3389/fgene.2018.00691DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6338032PMC
January 2019
1 Read

Identification and functional characterization of IRAK-4 in grass carp (Ctenopharyngodon idellus).

Fish Shellfish Immunol 2019 Jan 24;87:438-448. Epub 2019 Jan 24.

Department of Bioscience, College of Life Science, Nanchang University, Nanchang, 330031, China. Electronic address:

IL-1R-associated kinase 4 (IRAK4), a central TIR signaling mediator in innate immunity, can initiate a cascade of signaling events and lead to induction of inflammatory target gene expression eventually. In the present study, we cloned and characterized an IRAK4 orthologue from grass carp (Ctenopharyngodon idella). The full length cDNA of CiIRAK4 was 2057 bp with an ORF of 1422 bp encoding a polypeptide of 472 amino acids. Read More

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http://dx.doi.org/10.1016/j.fsi.2019.01.031DOI Listing
January 2019
9 Reads

An amino acid duplication/insertion in the Bm126 gene of Bombyx mori nucleopolyhedrovirus alters viral gene expression as shown by differential gene expression analysis.

Arch Virol 2019 Jan 24. Epub 2019 Jan 24.

Jiangsu Key Laboratory of Sericultural Biology and Biotechnology, School of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang, 212018, Jiangsu, People's Republic of China.

Open reading frame (ORF) 126 (Bm126) of Bombyx mori nucleopolyhedrovirus (BmNPV) is not essential for viral replication, and two subtypes of this gene have been identified in China. The Bm126-SX subtype encodes a protein with a simple amino acid duplication/insertion relative to the Bm126-GD subtype; however, significant differences in the cytopathic effect and infectivity of viruses carrying these variant genes have been observed. To elucidate the cause of these differences, differential gene expression analysis was performed at the early stage of infection with viruses harbouring variants of Bm126. Read More

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http://dx.doi.org/10.1007/s00705-018-04144-2DOI Listing
January 2019

[The underestimated coding potential of mitochondrial DNA].

Med Sci (Paris) 2019 Jan 23;35(1):46-54. Epub 2019 Jan 23.

Département de sciences biologiques, université de Montréal, pavillon Marie-Victorin, faculté des arts et des sciences. CP 6128, succursale centre-ville, Montréal QC, H3C 3J7, Canada.

Mitochondria are ancient organelles that emerged from the endosymbiosis of free-living proto-bacteria. They still retain a semi-autonomous genetic system with a small genome. Mitochondrial DNA (mtDNA) codes for 13 essential proteins for the production of ATP, the sequences of which are relatively conserved across Metazoans. Read More

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https://www.medecinesciences.org/10.1051/medsci/2018308
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http://dx.doi.org/10.1051/medsci/2018308DOI Listing
January 2019
8 Reads

Human double negative T cells target lung cancer via ligand-dependent mechanisms that can be enhanced by IL-15.

J Immunother Cancer 2019 Jan 22;7(1):17. Epub 2019 Jan 22.

Toronto General Hospital Research Institute, University Health Network, Toronto, Ontario, Canada.

Background: The advents of novel immunotherapies have revolutionized the treatment of cancer. Adoptive cellular therapies using chimeric antigen receptor T (CAR-T) cells have achieved remarkable clinical responses in B cell leukemia and lymphoma but the effect on solid tumors including lung cancer is limited. Here we present data on the therapeutic potential of allogeneic CD3CD4CD8 double negative T (DNT) cells as a new cellular therapy for the treatment of lung cancer and underlying mechanisms. Read More

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https://jitc.biomedcentral.com/articles/10.1186/s40425-019-0
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http://dx.doi.org/10.1186/s40425-019-0507-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6343266PMC
January 2019
5 Reads

An Exonic Switch Regulates Differential Accession of microRNAs to the Cd34 Transcript in Atherosclerosis Progression.

Genes (Basel) 2019 Jan 21;10(1). Epub 2019 Jan 21.

Independent Researcher, Esplugues de Llobregat, 08950 Barcelona, Spain.

Background: CD34⁺ Endothelial Progenitor Cells (EPCs) play an important role in the recovery of injured endothelium and contribute to atherosclerosis (ATH) pathogenesis. Previously we described a potential atherogenic role for miR-125 that we aimed to confirm in this work.

Methods: Microarray hybridization, TaqMan Low Density Array (TLDA) cards, qPCR, and immunohistochemistry (IHC) were used to analyze expression of the miRNAs, proteins and transcripts here studied. Read More

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http://dx.doi.org/10.3390/genes10010070DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6356495PMC
January 2019
1 Read

A Victorivirus and Two Novel Mitoviruses Co-Infected the Plant Pathogen .

Viruses 2019 Jan 19;11(1). Epub 2019 Jan 19.

Hunan Provincial Key Laboratory for Biology and Control of Plant Diseases and Insect Pests, Hunan Agricultural University, Changsha 410128, China.

Three dsRNAs, in sizes of approximately 2.5⁻5 kbp, were detected in the plant pathogenic fungus strain CS-7.5-4. Read More

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http://dx.doi.org/10.3390/v11010083DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6356909PMC
January 2019
1 Read
3.279 Impact Factor

pik3r3b, a novel immune-related gene in Nile tilapia (Oreochromis niloticus): Identification, expression and analysis of antibacterial activity.

Fish Shellfish Immunol 2019 Jan 19. Epub 2019 Jan 19.

Key Lab of Sustainable Development of Marine Fisheries, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao, 266071, China; Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, Qingdao, 266237, China; Shandong Key Lab for Marine Fishery Biotechnology and Genetic Breeding, Qingdao, 266071, China. Electronic address:

A full-length cDNA encoding phosphatidylinositol 3-kinase regulatory subunit gamma b gene in Nile tilapia (Oreochromis niloticus), termed as On-pik3r3b, was identified and characterized in this study. The sequence analysis demonstrated that the full-length cDNA of On-pik3r3b was 2018 bp, containing a 5' untranslated region (UTR) of 171 bp, an open reading frame (ORF) of 1422 bp and a 3' UTR of 425 bp. Its protein sequence displayed a high degree of identity with other fish. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S10504648193000
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http://dx.doi.org/10.1016/j.fsi.2019.01.006DOI Listing
January 2019
8 Reads

Complete genome sequence and construction of an infectious full-length cDNA clone of celery latent virus - an unusual member of a putative new genus within the Potyviridae.

J Gen Virol 2019 Feb 22;100(2):308-320. Epub 2019 Jan 22.

1​Department Phytomedicine, Leibniz University Hannover, Institute of Horticultural Production Systems, Herrenhäuser Str. 2, 30419, Hannover, Germany.

Celery latent virus (CeLV) is an incompletely described plant virus known to be sap and seed transmissible and to possess flexuous filamentous particles measuring about 900 nm in length, suggesting it as a possible member of the family Potyviridae. Here, an Italian isolate of CeLV was transmitted by sap to a number of host plants and shown to have a single-stranded and monopartite RNA genome being 11 519 nucleotides (nts) in size and possessing some unusual features. The RNA contains a large open reading frame (ORF) that is flanked by a short 5' untranslated region (UTR) of 13 nt and a 3' UTR consisting of 586 nt that is not polyadenylated. Read More

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http://dx.doi.org/10.1099/jgv.0.001207DOI Listing
February 2019
1 Read

Analysis of genetic variations of heat shock proteins Hsp70 and Hsp90 in Isaria farinosa strains from the Yunnan province of China.

Folia Microbiol (Praha) 2019 Jan 21. Epub 2019 Jan 21.

Yunnan Herbal Laboratory, Institute of Herb Biotic Resources, School of Life Sciences, Yunnan University, Kunming, 650091, China.

In the present study, the cDNA sequences of Hsp70 and Hsp90 genes of Isaria farinosa (designated IFHSP70 and IFHSP90) were cloned and characterized using multiple techniques of molecular biology and bioinformatics. The genetic differentiation of the two genes was investigated among 10 geographically separated populations distributed in the Yunnan province. The complete sequence of the IFHSP70 cDNA had a length of 2158 bp, and contained an open reading frame (ORF) of 1962 bp, encoding a 71-kDa polypeptide comprising of 653 amino acids. Read More

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http://dx.doi.org/10.1007/s12223-018-00676-xDOI Listing
January 2019
2 Reads

Adenosine Deaminase Acting on RNA 1 Associates with Orf Virus OV20.0 and Enhances Viral Replication.

J Virol 2019 Jan 16. Epub 2019 Jan 16.

Graduate Institute of Microbiology and Public Health, National Chung Hsing University, 145 Xingda Road, Taichung, 402, Taiwan

Orf virus (ORFV) infects sheep and goats and is also an important zoonotic pathogen. The viral protein OV20.0 has been shown to suppress innate immunity by targeting the double-stranded RNA (dsRNA)-activated protein kinase (PKR) by multiple mechanisms. Read More

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http://jvi.asm.org/lookup/doi/10.1128/JVI.01912-18
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http://dx.doi.org/10.1128/JVI.01912-18DOI Listing
January 2019
4 Reads

Identification of a novel plant RNA virus species of the genus Amalgavirus in the family Amalgaviridae from chia (Salvia hispanica).

Genes Genomics 2019 Jan 16. Epub 2019 Jan 16.

Department of Life Science, Chung-Ang University, 84 Heukseok-ro, Dongjak-gu, Seoul, 06974, South Korea.

Background: Chia (Salvia hispanica) is a flowering plant in the family Lamiaceae, which produces seeds that are a rich source of various nutritional compounds.

Objective: To identify a novel RNA virus potentially associated with chia.

Methods: Transcriptome data obtained from developing chia seeds were assembled into contigs. Read More

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http://link.springer.com/10.1007/s13258-019-00782-1
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http://dx.doi.org/10.1007/s13258-019-00782-1DOI Listing
January 2019
4 Reads

Characterization and identification of long non-coding RNAs based on feature relationship.

Bioinformatics 2019 Jan 12. Epub 2019 Jan 12.

CAS Key Laboratory of Genome Sciences and Information, Beijing Institute of Genomics, Chinese Academy of Sciences, Beijing, China.

Motivation: The significance of long non-coding RNAs (lncRNAs) in many biological processes and diseases has gained intense interests over the past several years. However, computational identification of lncRNAs in a wide range of species remains challenging; it requires prior knowledge of well-established sequences and annotations or species-specific training data, but the reality is that only a limited number of species have high-quality sequences and annotations.

Results: Here we first characterize lncRNAs by contrast to protein-coding RNAs based on feature relationship and find that the feature relationship between ORF (open reading frame) length and GC content presents universally substantial divergence in lncRNAs and protein-coding RNAs, as observed in a broad variety of species. Read More

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https://academic.oup.com/bioinformatics/advance-article/doi/
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http://dx.doi.org/10.1093/bioinformatics/btz008DOI Listing
January 2019
5 Reads

Ribosomal peptides and small proteins on the rise.

Chembiochem 2019 Jan 16. Epub 2019 Jan 16.

TU Munich Chair of Biochemistry, Chemistry, Lichtenbergstr. 4, 85747, Garching, GERMANY.

Genetically encoded and ribosomally synthesised small proteins and peptides act as important regulators in fundamental cellular processes, including gene expression, development, signalling and metabolism. Moreover, they also play a crucial role in the eukaryotic and prokaryotic defence against microorganisms and are extremely diverse in size and structure, and are often subject to extensive posttranslational modification. Recent technological advances now allow the analysis of the whole cellular transcriptome and proteome, revealing the presence of hundreds, long overlooked, alternative and short open reading frames (ORFs) in mRNA and supposedly non-coding RNAs. Read More

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http://doi.wiley.com/10.1002/cbic.201800715
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http://dx.doi.org/10.1002/cbic.201800715DOI Listing
January 2019
4 Reads

B cell receptor accessory molecule CD79 gets involved in response against Streptococcus agalactiae infection and BCR signaling in Nile tilapia (Oreochromis niloticus).

Fish Shellfish Immunol 2019 Jan 12;87:212-219. Epub 2019 Jan 12.

School of Life Sciences, South China Normal University, Institute of Modern Aquaculture Science and Engineering, Guangdong Provincial Key Laboratory for Healthy and Safe Aquaculture, Guangzhou, 510631, PR China. Electronic address:

CD79, composed of two distinct chains called CD79a and CD79b, is a transmembrane protein that forms a B cell antigen receptor with membrane immunoglobulin, and generates a signal following antigen recognition by the B cell receptor. In this study, the CD79a (OnCD79a) and CD79b (OnCD79b) were cloned and identified from Nile tilapia (Oreochromis niloticus). The cDNA of ORF for OnCD79a and OnCD79b are 669 and 627 bp, coding 222 and 208 amino acids, respectively. Read More

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http://dx.doi.org/10.1016/j.fsi.2019.01.012DOI Listing
January 2019
1 Read

Characterization of the ScghC1q-1 gene in Sinonovacula constricta and its role in innate immune responses.

Dev Comp Immunol 2019 May 11;94:16-21. Epub 2019 Jan 11.

Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Education, Shanghai Ocean University, Shanghai, 201306, China; Shanghai Engineering Research Center of Aquaculture, Shanghai, 201306, China. Electronic address:

C1q is an important immune gene that can mediate a variety of immune regulatory functions, and is involved in complement pathway activation. In the present study, a ghC1q gene from the razor clam Sinonovacula constricta was identified and named ScghC1q-1. The complete ScghC1q-1 gene is 692 bp in length, with an open reading frame (ORF) of 489 bp encoding a protein of 162 amino acids. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S0145305X183052
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http://dx.doi.org/10.1016/j.dci.2019.01.004DOI Listing
May 2019
4 Reads
2.815 Impact Factor

Identification of a novel C1q complement component in razor clam Sinonovacula constricta and its role in antibacterial activity.

Fish Shellfish Immunol 2019 Jan 11;87:193-201. Epub 2019 Jan 11.

Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Education, Shanghai Ocean University, Shanghai, 201306, China; Shanghai Engineering Research Centre of Aquaculture, Shanghai, 201306, China. Electronic address:

The serum complement component C1q mediates a variety of immune regulatory functions. Herein, we identified a globular head C1q (ghC1q) gene in razor clam Sinonovacula constricta. The complete Sc-ghC1q gene was 872 bp long included an 81 bp 5'-untranslated region (UTR), a 95 bp 3'-UTR with a poly(A) tail, and an open reading frame (ORF) of 696 bp. Read More

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http://dx.doi.org/10.1016/j.fsi.2019.01.014DOI Listing
January 2019
2.674 Impact Factor