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    A conserved interferon regulation factor 1 (IRF-1) from Pacific oyster Crassostrea gigas functioned as an activator of IFN pathway.
    Fish Shellfish Immunol 2018 Feb 16. Epub 2018 Feb 16.
    Liaoning Key Laboratory of Marine Animal Immunology, Dalian Ocean University, Dalian 116023, China; Laboratory of Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266235, China; Liaoning Key Laboratory of Marine Animal Immunology & Disease Control, Dalian Ocean University, Dalian 116023, China.
    Interferon regulatory factors (IRFs), a family of transcription factors with a novel helix-turn-helix DNA-binding motif, play important roles in regulating the expression of interferons (IFNs) and IFN-stimulated genes. In the present study, an interferon regulation factor 1 was identified from oyster Crassostrea gigas (designated CgIRF-1), and its immune function was characterized to understand the regulatory mechanism of interferon system against viral infection in invertebrates. The open reading frame (ORF) of CgIRF-1 was 993 bp, encoding a polypeptide of 330 amino acids with a typical IRF domain (also known as DNA-binding domain). Read More

    The Coding Regions of Germline mRNAs Confer Sensitivity to Argonaute Regulation in C. elegans.
    Cell Rep 2018 Feb 14. Epub 2018 Feb 14.
    RNA Therapeutics Institute, UMass Medical School, 368 Plantation Street, Worcester, MA 01605, USA; Howard Hughes Medical Institute. Electronic address:
    Protein-coding genes undergo a wide array of regulatory interactions with factors that engage non-coding regions. Open reading frames (ORFs), in contrast, are thought to be constrained by coding function, precluding a major role in gene regulation. Here, we explore Piwi-interacting (pi)RNA-mediated transgene silencing in C. Read More

    Cloning and expression analysis of cDNAs encoding ADP-glucose pyrophosphorylase large and small subunits from hulless barley (Hordeum vulgare L. var. nudum).
    Z Naturforsch C 2018 Feb 19. Epub 2018 Feb 19.
    College of Medical Technology, Chengdu University of Traditional Chinese Medicine, Chengdu 611137, P.R. China.
    As an important plateau cereal crop, hulless barley is the principal food for the Tibetan people in China. ADP-glucose pyrophosphorylase (AGPase) is considered as the key enzyme for starch biosynthesis in plants. In this study, cDNAs encoding the small subunit (SSU I) and large subunit (LSU I) of AGPase were isolated from hulless barley. Read More

    Cloning, molecular modeling and characterization of acidic cellulase from buffalo rumen and its applicability in saccharification of lignocellulosic biomass.
    Int J Biol Macromol 2018 Feb 15. Epub 2018 Feb 15.
    Department of Animal Biotechnology, College of Veterinary Science and Animal Husbandry, Anand Agricultural University, Anand 388110, Gujarat, India.
    Cellulase hydrolyses the cellulose by cleaving the β-1,4-linkages to produce mono-, oligo- and shorter polysaccharide units. These enzymes have applications in various industries such as pulp and paper, laundry, food and feed, textile, brewing industry and in biofuel production. In the present study we have cloned acid-cellulase gene (Cel-1) from the fosmid library of buffalo rumen metagenomic DNA and functionally expressed it in Escherichia coli. Read More

    Identification and functional analysis of inhibitor of NF-κB kinase (IKK) from Scylla paramamosain: The first evidence of three IKKs in crab species and their expression profiles under biotic and abiotic stresses.
    Dev Comp Immunol 2018 Feb 15. Epub 2018 Feb 15.
    College of Animal Sciences, Zhejiang University, Hangzhou 310058, China. Electronic address:
    IKK (inhibitor of NF-κB kinase) is the core regulator for NF-κB (nuclear factor-κB) pathway against pathogenic invasion in vertebrates or invertebrates. However, the IKK from crab species has not yet been identified. In the present study, three full-length cDNA sequences of IKKs from mud crab Scylla paramamosain, designated as SpIKKβ, SpIKKε1 and SpIKKε2, were firstly cloned through RT-PCR and RACE methods. Read More

    Isolation of a novel Rhabdovirus from an insectivorous bat (Pipistrellus kuhlii) in Italy.
    Virol J 2018 Feb 17;15(1):37. Epub 2018 Feb 17.
    Istituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia Romagna (IZSLER), Via Bianchi 9 -, 25124, Brescia, Italy.
    Background: Rhabdoviridae is one of the most ecologically diverse families of RNA viruses which can infect a wide range of vertebrates and invertebrates. Bats, among mammals, are pointed to harbor a significantly higher proportion of unknown or emerging viruses with zoonotic potential. Herein, we report the isolation of a novel rhabdovirus, detected in the framework of a virological survey on bats implemented in North Italy. Read More

    Identification of two novel CRESS DNA viruses associated with an Avipoxvirus lesion of a blue-and-gray Tanager (Thraupis episcopus).
    Infect Genet Evol 2018 Feb 14. Epub 2018 Feb 14.
    Department of Physiology, Genetic and Microbiology, Faculty of Biology, Complutense University of Madrid, Calle José Antonio Nováis 12, 28040,Madrid, Spain. Electronic address:
    The discovery of circular rep-encoding single stranded (CRESS) DNA viruses has increased spectacularly over the past decade. They represent the smallest animal viruses known worldwide infecting a wide variety of invertebrates and vertebrates in different natural and human-made environments. The extremely low similarity of nucleotide and protein sequences among different CRESS DNA genomes has challenged their classification. Read More

    Hepatitis C virus core+1/ARFP modulates Cyclin D1/pRb pathway and promotes carcinogenesis.
    J Virol 2018 Feb 14. Epub 2018 Feb 14.
    Laboratory of Biochemistry and Molecular Virology, Department of Molecular Biology and Genetics, Democritus University of Thrace, Alexandroupolis, Greece
    Viruses often encompass overlapping reading frames and unconventional translation mechanisms in order to maximize the output from a minimum genome and to orchestrate timely their gene expression. HCV possesses such an unconventional open reading frame (ORF) within the core-coding region, encoding an additional protein designated initially as ARFP or F or core+1. Two predominant isoforms of core+1/ARFP have been reported, core+1/L initiating from codon 26 and core+1/S initiating from codons 85/87 of the polyprotein coding region, respectively. Read More

    Expression of PLP-independent racemases can reduce 2-aminoacrylate stress in.
    J Bacteriol 2018 Feb 12. Epub 2018 Feb 12.
    Department of Microbiology, University of Georgia, Athens, GA 30602
    The RidA protein (PF01042) fromis a deaminase that quenches 2-aminoacrylate (2AA), and other reactive metabolites. In the absence of RidA, 2AA accumulates, damages cellular enzymes and compromises the metabolic network., RidA homologs from all domains of life deaminate 2-aminoacrylate and RidA proteins from plants, bacteria, yeast, and humans complement the mutant phenotypes of amutant strain ofIn the present study a methanogenic archaeon,S2, was used to probe alternative mechanisms to restore metabolic balance. Read More

    Diversification of Secondary Metabolite Biosynthetic Gene Clusters Coincides with Lineage Divergence in Streptomyces.
    Antibiotics (Basel) 2018 Feb 13;7(1). Epub 2018 Feb 13.
    School of Integrative Plant Science, Bradfield Hall 705, Cornell University, Ithaca, NY 14853, USA.
    We have identifiedsister-taxa which share a recent common ancestor and nearly identical small subunit (SSU) rRNA gene sequences, but inhabit distinct geographic ranges demarcated by latitude and have sufficient genomic divergence to represent distinct species. Here, we explore the evolutionary dynamics of secondary metabolite biosynthetic gene clusters (SMGCs) following lineage divergence of these sister-taxa. These sister-taxa strains contained 310 distinct SMGCs belonging to 22 different gene cluster classes. Read More

    Chickenpox outbreak in a tribal and industrial zone from the Union Territory of Dadra and Nagar Haveli, India.
    Epidemiol Infect 2018 Feb 13:1-5. Epub 2018 Feb 13.
    ICMR-National Institute of Virology,20-A Dr Ambedkar Road,Pune 411001,India.
    During 9th December 2016 and 12th February 2017, 149-chickenpox cases were reported in a tribal and industrial zone of Rakholi (n = 80) and Surangi (n = 69) villages from Union Territory of India. An epidemiological investigation was performed to assess the characteristics and determinants of the chickenpox outbreak. Overall, the attack rate per 100 population in Rakholi village (n = 1757) was 4. Read More

    Dynamics of a geminivirus encoded pre-coat protein and host RNA- dependent RNA polymerase 1 in regulating symptom recovery in tobacco.
    J Exp Bot 2018 Feb 8. Epub 2018 Feb 8.
    Molecular Virology Laboratory, School of Life Sciences, Jawaharlal Nehru University, New Delhi, India.
    RNA silencing is an integral part of the plant's cellular defense mechanism acting against virus infection. However, explicit role of RNA silencing and interplay between host and virus components during the host recovery from geminivirus infection is still unexplored. Hence, in this study we aimed to examine the mechanism behind the host-specific recovery of Nicotiana tabacum (cv. Read More

    Parapoxviral vulvovaginitis in Holstein cows.
    J Vet Diagn Invest 2018 Feb 1:1040638718758829. Epub 2018 Feb 1.
    California Animal Health and Food Safety Laboratory, Tulare Laboratory (Moeller, Adaska, Blanchard), School of Veterinary Medicine, University of California, Davis, CA.
    A group of Holstein first-calved heifers developed small pustules and ulcers on the vulva and in the vagina during the first 1-4 wk postpartum. The lesions varied from small red pinpoint foci to pustules and ulcers, 3-5 mm diameter. Some ulcers coalesced to form large ulcerated areas up to 15 mm diameter. Read More

    Characterization of a cryptic plasmid isolated from Lactobacillus casei CP002616 and construction of shuttle vectors based on its replicon.
    J Dairy Sci 2018 Feb 7. Epub 2018 Feb 7.
    Shanghai Engineering Research Center of Food Microbiology, School of Medical Instrument and Food Engineering, University of Shanghai for Science and Technology, Shanghai 200093, China; Key Lab of Synthetic Biology, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200032, China. Electronic address:
    The cryptic plasmid pLC2W was isolated from Lactobacillus casei CP002616. Nucleotide sequence analysis revealed that 4 putative open reading frames (ORF) were responsible for DNA replication. Four Escherichia coli-Lactobacillus shuttle vectors were constructed using different lengths of the pLC2W replicon to identify the shortest functional replicon. Read More

    Identification and functional characterization of unfolded protein response transcription factor ATF6 gene in kuruma shrimp Marsupenaeus japonicus.
    Fish Shellfish Immunol 2018 Feb 7;75:223-230. Epub 2018 Feb 7.
    Key Laboratory of Tumour Molecular Biology, Department of Clinical Medicine, Binzhou Medical University, Yantai, 264003, China. Electronic address:
    Activating transcription factor 6 (ATF6) pathway is the key branch of unfolded protein response (UPR). In this study, a homolog of ATFα from Marsupenaeus japonicus (MjATF6) was identified using genome sequencing and characterized, so as to investigate the role of ATF6 pathway in anti-viral immunity of M. japonicus. Read More

    Two I84 family protease inhibitors from Chinese razor clams Sinonovacula constricta expressed in response to environmental challenges.
    Fish Shellfish Immunol 2018 Feb 8;75:149-157. Epub 2018 Feb 8.
    Key Laboratory of Aquatic Germplasm Resource of Zhejiang, Zhejiang Wanli University, Ningbo, Zhejiang 315100, China. Electronic address:
    Protease inhibitors play critical roles in numerous biological processes including host defense in all multicellular organisms. Eighty three evolutionary families of protease inhibitors are currently accommodated in the MEROPS database and the I84 family currently consists of 3 novel serine protease inhibitors from the eastern oyster Crassostrea virginica. In this study, we identified 2 new I84 family members from the Chinese razor clam Sinonovacula constricta, scSI-1 and scSI-2, using cDNA cloning and sequencing. Read More

    Phage display antibodies against ectromelia virus that neutralize variola virus: Selection and implementation for p35 neutralizing epitope mapping.
    Antiviral Res 2018 Feb 7;152:18-25. Epub 2018 Feb 7.
    Institute of Chemical Biology and Fundamental Medicine, Siberian Branch of the Russian Academy of Sciences, Novosibirsk, 630090, Russia. Electronic address:
    In this study, five phage display antibodies (pdAbs) against ectromelia virus (ECTV) were selected from vaccinia virus (VACV)-immune phage-display library of human single chain variable fragments (scFv). ELISA demonstrated that selected pdAbs could recognize ECTV, VACV, and cowpox virus (CPXV). Atomic force microscopy visualized binding of the pdAbs to VACV. Read More

    A novel dual-motif enables two-way autoregulation of CcpA in.
    Appl Environ Microbiol 2018 Feb 2. Epub 2018 Feb 2.
    Key Laboratory of Synthetic Biology, CAS Center for Excellence in Molecular Plant Sciences, Shanghai Institute of Plant Physiology and Ecology, Chinese Academy of Sciences, Shanghai 200032, China
    The master regulator CcpA (catabolite control protein A) manages a large and complex regulatory network that is essential for cellular physiology and metabolism in gram-positive bacteria. Although CcpA can affect the expression of target genes by binding to a-acting catabolite responsive element (), whether and how the expression of CcpA is regulated remains poorly explored. Here, we report a novel dual-motif that is employed by the CcpA in, a typical solventogenicspecies, for autoregulation. Read More

    Spinocerebellum Ataxia Type 6: Molecular Mechanisms and Calcium Channel Genetics.
    Adv Exp Med Biol 2018 ;1049:147-173
    Department of Neurology, The University of Chicago, Chicago, 60637, IL, USA.
    Spinocerebellar ataxia (SCA) type 6 is an autosomal dominant disease affecting cerebellar degeneration. Clinically, it is characterized by pure cerebellar dysfunction, slowly progressive unsteadiness of gait and stance, slurred speech, and abnormal eye movements with late onset. Pathological findings of SCA6 include a diffuse loss of Purkinje cells, predominantly in the cerebellar vermis. Read More

    Duck hepatitis A virus structural proteins expressed in insect cells self-assemble into virus-like particles with strong immunogenicity in ducklings.
    Vet Microbiol 2018 Feb 26;215:23-28. Epub 2017 Dec 26.
    Jiangsu Agri-animal Husbandry Vocational College, Jiangsu Key Laboratory for High-Tech Research and Development of Veterinary Biopharmaceuticals, Taizhou, 225300, China. Electronic address:
    Duck hepatitis A virus (DHAV), a non-enveloped ssRNA virus, can cause a highly contagious disease in young ducklings. The three capsid proteins of VP0, VP1 and VP3 are translated within a single large open reading frame (ORF) and hydrolyzed by protease 3CD. However, little is known on whether the recombinant viral structural proteins (VPs) expressed in insect cells could spontaneously assemble into virus-like particles (VLPs) and whether these VLPs could induce protective immunity in young ducklings. Read More

    Immune function against bacteria of chitin deacetylase 1 (EcCDA1) from Exopalaemon carinicauda.
    Fish Shellfish Immunol 2018 Feb 5;75:115-123. Epub 2018 Feb 5.
    Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266000, China; Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China.
    Chitin deacetylase (CDA, EC 3.5.1. Read More

    High variability within Candida albicans transcription factor RLM1: Isolates from vulvovaginal infections show a clear bias toward high molecular weight alleles.
    Med Mycol 2017 Oct 9. Epub 2017 Oct 9.
    Centre of Molecular and Environmental Biology (CBMA), Department of Biology, University of Minho, Braga, Portugal.
    Previous studies have correlated the severity of recurrent vulvovaginal Candida infections (VVC) and balanitis in patients from China with the presence of some dominant genotypes at the ORF RLM1. Here we tested VVC vs non-VVC isolates from Portugal, Brazil and Greece and, although the same genotypes were identified in VVC isolates, they were present in only five out of 150 strains. However, this analysis showed that VVC isolates presented a higher percentage of genotypes with similar high molecular weight alleles, in comparison with strains isolated from other biological sources. Read More

    The Regulation of Translation in Alphavirus-Infected Cells.
    Viruses 2018 Feb 8;10(2). Epub 2018 Feb 8.
    Centro de Biología Molecular Severo Ochoa (CSIC-UAM), Universidad Autónoma de Madrid c/Nicolás Cabrera, 1., Cantoblanco, 28049 Madrid, Spain.
    Sindbis virus (SINV) contains an RNA genome of positive polarity with two open reading frames (ORFs). The first ORF is translated from the genomic RNA (gRNA), rendering the viral non-structural proteins, whereas the second ORF is translated from a subgenomic mRNA (sgRNA), which directs the synthesis of viral structural proteins. SINV infection strongly inhibits host cell translation through a variety of different mechanisms, including the phosphorylation of the eukaryotic initiation factor eIF2α and the redistribution of cellular proteins from the nucleus to the cytoplasm. Read More

    Bacillus subtilis Type I antitoxin SR6 Promotes Degradation of Toxin yonT mRNA and Is Required to Prevent Toxic yoyJ Overexpression.
    Toxins (Basel) 2018 Feb 7;10(2). Epub 2018 Feb 7.
    Friedrich-Schiller-Universität Jena, Lehrstuhl für Genetik, AG Bakteriengenetik, Philosophenweg 12, D-07743 Jena, Germany.
    /SR6 is the second type I toxin-antitoxin (TA) system encoded on prophage SPβ in thechromosome. TheORF specifying a 58 aa toxin is transcribed on a polycistronic mRNA under control of thepromoter. The antitoxin SR6 is a 100 nt antisense RNA that overlapsat its 3' end and the downstream geneencoding a second, much weaker, toxin at its 5' end. Read More

    Interferon induced Mx protein from Indian snow trout Schizothorax richardsonii (Gray) lacks critical functional features unlike its mammalian homologues.
    Comput Biol Chem 2018 Jan 31;73:31-40. Epub 2018 Jan 31.
    ICAR-Directorate of Coldwater Fisheries Research, Anusandhan Bhawan, Industrial Area, Bhimtal, Nainital 263139, Uttarakhand, India. Electronic address:
    Viral attack within host cells triggers the production of type I interferons and leads to the induction of interferon stimulated genes (ISGs). One of the ISG Mx, encodes type I interferon inducible GTPase that is responsible for the establishment of an anti-viral state within cells. Intriguingly, several isoforms of Mx have been reported in fish, but the structural analysis of fish Mx proteins remains unexplored. Read More

    Characterization of a novel lytic bacteriophage from an industrial Escherichia coli fermentation process and elimination of virulence using a heterologous CRISPR-Cas9 system.
    J Ind Microbiol Biotechnol 2018 Mar 7;45(3):153-163. Epub 2018 Feb 7.
    DuPont Tate & Lyle Bio Products, 198 Blair Bend Drive, Loudon, TN, 37774, USA.
    Bacterial-bacteriophage interactions are a well-studied and ecologically-important aspect of microbiology. Many commercial fermentation processes are susceptible to bacteriophage infections due to the use of high-density, clonal cell populations. Lytic infections of bacterial cells in these fermentations are especially problematic due to their negative impacts on product quality, asset utilization, and fouling of downstream equipment. Read More

    The comprehensive expression analysis of the G protein-coupled receptor from Penaeus monodon indicating it participates in innate immunity and anti-ammonia nitrogen stress.
    Fish Shellfish Immunol 2018 Feb 1. Epub 2018 Feb 1.
    South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Key Laboratory of South Ch0ina Sea Fishery Resources Exploitation and Utilization, Ministry of Agriculture, Guangzhou, 510300, PR China; Shenzhen Base of South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Shenzhen, 518108, PR China. Electronic address:
    The G protein-coupled receptors (GPCRs) composed a superfamily that played an important role in physiological processes of crustaceans, with multiple functions such as growth and development, acting as a defense against stimulations from external factors. In this paper, one kind of GPCRs were identified from Penaeus monodon, called PmGPCR, included an open reading frame (ORF) of 1113 bp. Bioinformatic analysis showed that PmGPCR protein had the typical structure of seven transmembrane domains (7TM), especially the special Asp-Arg-Try motif (DRY motif) between the third transmembrane structures (TM3) and the second intracellular loops (IL-2) which can prove that PmGPCR belongs to the rhodopsin-like family. Read More

    CpMCA, a novel metacaspase gene from the harmful dinoflagellate Cochlodinium polykrikoides and its expression during cell death.
    Gene 2018 Feb 2. Epub 2018 Feb 2.
    Department of Biotechnology, Sangmyung University, Seoul 03016, South Korea. Electronic address:
    Metacaspases (MCAs) are cysteine proteases that share sequence homology with caspases, and may play roles in programmed cell death (PCD). In the present study, we identified a novel MCA gene (CpMCA) from the red tide dinoflagellate Cochlodinium polykrikoides, and examined its molecular characteristics and gene expression in response to algicide-induced cell death. CpMCA cDNA is 1164 bp in length, containing a dinoflagellate spliced leader sequence (dinoSL), an 879-bp open reading frame (ORF), which codes for a 293-aa protein, and a poly (A) tail. Read More

    Molecular characterization and expression of toll-like receptor 5 genes from Pelteobagrus vachellii.
    Fish Shellfish Immunol 2018 Feb 3;75:198-207. Epub 2018 Feb 3.
    College of Life Science, Neijiang Normal University, Key Laboratory of Sichuan Province for Fishes Conservation and Utilization in the Upper Reaches of the Yangtze River, Neijiang 641000, PR China.
    Toll-like receptor 5 (TLR5) is an important pathogen recognition receptor (PRR) that recognizes the flagellin protein of pathogenic bacteria and plays a fundamental role in activating the innate immune response. In this study, full-length pvTLR5m (membrane) and pvTLR5s (soluble) genes were cloned from darkbarbel catfish Pelteobagrus vachellii, and their expression and that of downstream genes were analyzed following exposure to the Aeromonas hydrophila pathogen. The 3009 bp pvTLR5m cDNA includes a 2652 bp open reading frame (ORF) encoding 884 amino acids. Read More

    Isolation of a laccase-coding gene from the lignin-degrading fungus Phlebia brevispora BAFC 633 and heterologous expression in Pichia pastoris.
    J Appl Microbiol 2018 Feb 6. Epub 2018 Feb 6.
    Laboratorio de Biotecnología Molecular, Instituto de Biotecnología Misiones, Facultad de Ciencias Exactas Químicas y Naturales, Universidad Nacional de Misiones, Ruta 12 Km 7,5 - Campus Universitario UNaM - 3304, Posadas, Misiones, Argentina.
    Aims: Isolate and characterize a laccase-encoding gene (lac I) of Phlebia brevispora BAFC 633, as well as cloning and expressing cDNA of lac I in Pichia pastoris. And to obtain a purified and characterized recombinant laccase to analyze the biotechnological application potential.

    Methods And Results: lac I was cloned and sequenced, it contains 2447 pb obtained by PCR and long-distance inverse PCR. Read More

    Spexin in the half-smooth tongue sole (Cynoglossus semilaevis): molecular cloning, expression profiles, and physiological effects.
    Fish Physiol Biochem 2018 Feb 5. Epub 2018 Feb 5.
    Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, No. 106 Nanjing Road, Qingdao, 266071, China.
    Spexin (SPX), a novel neuropeptide discovered by the bioinformatics approach, has been shown to exert pleiotropic functions in mammals. However, little information regarding the physiological role of SPX is available in teleosts. As a first step, we cloned the spexin gene from a flatfish, the half-smooth tongue sole. Read More

    Acute hepatitis E in India appears to be caused exclusively by genotype 1 hepatitis E virus.
    Indian J Gastroenterol 2018 Feb 5. Epub 2018 Feb 5.
    Department of Gastroenterology, Sanjay Gandhi Postgraduate Institute of Medical Sciences, Raebareli Road, Lucknow, 226 014, India.
    Background: Hepatitis E is caused by infection with hepatitis E virus (HEV), which has four well-known genotypes. Genotypes 1 and 2 HEV have been reported from human cases in areas where the disease is highly endemic. By contrast, genotypes 3 and 4 HEV, which primarily infect several animal species worldwide, have been reported mainly from sporadic human cases in non-endemic areas such as Japan and high-income countries of Europe and North America. Read More

    [Effect of HIF-2α on the biological characteristics of breast cancer stem cells].
    Zhonghua Yi Xue Za Zhi 2018 Jan;98(4):269-273
    Department of Pathology, the First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China.
    To explore the effect of hypoxia inducible factor (HIF)-2α on biological characteristics of breast cancer stem cells (BCSCs).Stem cells were isolated and purified from MCF-7 cells by using the immunomagnetic microbeads. HIF-2α ORF/shRNA lentiviral vectors were transduced into MCF-7 stem cells respectively, and then the stable stem cell lines were detected and gained. Read More

    Molecular surveillance of norovirus, 2005-16: an epidemiological analysis of data collected from the NoroNet network.
    Lancet Infect Dis 2018 Jan 25. Epub 2018 Jan 25.
    Department of Viroscience, Erasmus Medical Center, Rotterdam, Netherlands; Centre for Infectious Diseases Research, Diagnostics and Screening, National Institute of Public Health and the Environment, Bilthoven, Netherlands. Electronic address:
    Background: The development of a vaccine for norovirus requires a detailed understanding of global genetic diversity of noroviruses. We analysed their epidemiology and diversity using surveillance data from the NoroNet network.

    Methods: We included genetic sequences of norovirus specimens obtained from outbreak investigations and sporadic gastroenteritis cases between 2005 and 2016 in Europe, Asia, Oceania, and Africa. Read More

    The first complete genome sequence of narcissus latent virus from Narcissus.
    Arch Virol 2018 Feb 1. Epub 2018 Feb 1.
    Fujian Key Laboratory for Technology Research of Inspection and Quarantine, Inspection and Quarantine Technology Center, Fujian Exit-Entry Inspection and Quarantine Bureau, Fuzhou, 350001, People's Republic of China.
    The complete sequence of a narcissus virus isolated from the Netherlands (Narv-NL) was determined to be 8172 nucleotides in length with an open reading frame encoding for 2624 amino acids. Phylogenetic analysis indicates that Narv-NL is clustered with high confidence among representative members from the genus Macluravirus, including artichoke latent virus (ArLV) and Chinese yam necrotic mosaic virus (CYNMV). Sequence analyses indicated Narv-NL shares 67%-69% nucleotide and 51%-68% amino acid sequence identity with ArLV and CYNMV either in the complete ORF or the coat protein (CP) gene, whereas it had 81%-99 % nucleotide and 80%-99 % amino acid sequence identity with the corresponding CP sequences of narcissus latent virus (NLV) isolates, suggesting that Narv-NL is a member of NLV. Read More

    PPARβ in yellow catfish Pelteobagrus fulvidraco: molecular characterization, tissue expression and transcriptional regulation by dietary Cu and Zn.
    Fish Physiol Biochem 2018 Feb 1. Epub 2018 Feb 1.
    Key Laboratory of Freshwater Animal Breeding, Ministry of Agriculture of P.R.C., Fishery College, Huazhong Agricultural University, Wuhan, 430070, China.
    Peroxisome proliferator-activated receptor beta (PPARβ) is a ligand-activated transcription factor that plays critical roles in the regulation of many important physiological processes. In this study, PPARβ was cloned and characterized in yellow catfish Pelteobagrus fulvidraco. PPARβ cDNA was 2350 bp in length with an open reading frame (ORF) of 1530 bp, encoding 509 amino acids, a 5'-untranslated region (UTR) of 474 bp, and a 3'-UTR of 346 bp. Read More

    A presumed homologue of the regulatory subunits of eIF2B functions as ribose-1,5-bisphosphate isomerase in Pyrococcus horikoshii OT3.
    Sci Rep 2018 Jan 30;8(1):1891. Epub 2018 Jan 30.
    Department of Biosciences and Bioengineering, Indian Institute of Technology Guwahati, Guwahati, 781039, Assam, India.
    The homologues of the regulatory subunits of eukaryotic translation initiation factor 2B (eIF2B) are assumed to be present in archaea. Likewise, an ORF, PH0208 in Pyrococcus horikoshii OT3 have been proposed to encode one of the homologues of regulatory subunits of eIF2B. However, PH0208 protein also shares sequence similarity with a functionally non-related enzyme, ribose-1,5-bisphosphate isomerase (R15Pi), involved in conversion of ribose-1,5-bisphosphate (R15P) to ribulose-1,5-bisphosphate (RuBP) in an AMP-dependent manner. Read More

    Impact of Multiple Single-Nucleotide Polymorphisms Within mprF on Daptomycin Resistance in Staphylococcus aureus.
    Microb Drug Resist 2018 Jan 30. Epub 2018 Jan 30.
    2 Los Angeles Biomedical Research Institute at Harbor-UCLA Medical Center , Torrance, California.
    A number of single nucleotide polymorphisms (SNPs) within the mprF open reading frame (ORF) have been associated with daptomycin-resistance (DAP-R) in Staphylococcus aureus. Such SNPs have been found throughout the mprF ORF, although there are clearly preferred "hot spots" within this gene frequently linked to DAP-R phenotype. These mprF SNPs are often correlated with a gain-in-function phenotype, either in terms of increased production (synthase activity) and/or enhanced translocation (translocase activity) of lysyl-phosphatidylglycerol (L-PG) within its cell membrane. Read More

    dsRNA targeting calmodulin reveal a potential target for pest management of Nilaparvata lugens.
    Pest Manag Sci 2018 Jan 30. Epub 2018 Jan 30.
    State Key Lab of Rice Biology, China Rice Research Institute, Hangzhou, Zhejiang, 310006, China.
    Background: Calmodulin (CaM) is an essential protein in cellular activity and play important roles in many processes in insect development. RNAi have been hypothesized to be promising methods for pest control. However, the sequence and function of CaM in Nilaparvata lugens is unknown. Read More

    Detection and Characterization of Hepatitis E Virus in Goats at Slaughterhouse in Tai'an Region, China.
    Biomed Res Int 2017 12;2017:3723650. Epub 2017 Dec 12.
    College of Life Sciences, Taishan Medical University, Tai'an 271000, China.
    Background: Hepatitis E virus (HEV) is a significant pathogen of viral hepatitis and can be transmitted through fecal-oral route. Epidemiological data concerning HEV in goats, however, are relatively sparse to date. Here, the prevalence and characteristics of HEV isolated from goats at slaughterhouse were investigated in Tai'an region, China. Read More

    RACKI induces chemotherapy resistance in esophageal carcinoma by upregulating the PI3K/AKT pathway and Bcl-2 expression.
    Onco Targets Ther 2018 4;11:211-220. Epub 2018 Jan 4.
    Department of Radiation Oncology, Qilu Hospital of Shandong University, Jinan, Shandong, People's Republic of China.
    Introduction: Accumulating evidence indicates that RACK1 is involved in the progression of tumors. We aimed to evaluate the function of RACK1 in esophageal squamous cell carcinoma (ESCC) and its role in the mechanism of chemotherapy resistance.

    Materials And Methods: Transfected ESCC cell lines with plasmids expressed shRACK1 or open reading frame (ORF) targeting RACK1 and established stable cell lines. Read More

    Antioxidative properties and structural features of atypical 2-Cys peroxiredoxin from Sebastes schlegelii.
    Dev Comp Immunol 2018 Jan 31;82:152-164. Epub 2018 Jan 31.
    Department of Marine Life Sciences & Fish Vaccine Research Center, Jeju National University, Jeju Self-Governing Province, 63243, Republic of Korea; Marine Science Institute, Jeju National University, Jeju Self-Governing Province, 63333, Republic of Korea. Electronic address:
    Atypical 2-Cys peroxiredoxin (Prx5) is an antioxidant protein that exerts its antioxidant function by detoxifying different reactive oxygen species (ROS). Here, we identified mitochondrial Prx5 from rockfish (SsPrx5) and described its specific structural and functional characteristics. The open reading frame (ORF) of SsPrx5 (570 bp) was translated into a 190-amino acid polypeptide that contained a mitochondrial targeting sequence (MTS), thioredoxin 2 domain, two Prx-specific signature motifs, and three conserved cysteine residues. Read More

    Role of TRP Channels in Dinoflagellate Mechanotransduction.
    Biol Bull 2017 Oct 3;233(2):151-167. Epub 2018 Jan 3.
    Transient receptor potential (TRP) ion channels are common components of mechanosensing pathways, mainly described in mammals and other multicellular organisms. To gain insight into the evolutionary origins of eukaryotic mechanosensory proteins, we investigated the involvement of TRP channels in mechanosensing in a unicellular eukaryotic protist, the dinoflagellate Lingulodinium polyedra. BLASTP analysis of the protein sequences predicted from the L. Read More

    Cloning and characterization of a specific UDP-glycosyltransferase gene induced by DON and Fusarium graminearum.
    Plant Cell Rep 2018 Jan 25. Epub 2018 Jan 25.
    State Key Laboratory of Crop Biology/Shandong Key Laboratory of Crop Biology, College of Agronomy, Shandong Agricultural University, 61 Daizong Street, Tai'an, 271018, Shandong, People's Republic of China.
    Key Message: TaUGT5: can reduce the proliferation and destruction of F. graminearum and enhance the ability of FHB resistance in wheat. Deoxynivalenol (DON) is one of the most important toxins produced by Fusarium species that enhances the spread of the pathogen in the host. Read More

    Identification of AUXIN RESPONSE FACTOR gene family from Prunus sibirica and its expression analysis during mesocarp and kernel development.
    BMC Plant Biol 2018 01 24;18(1):21. Epub 2018 Jan 24.
    Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants, College of Biological Sciences and Biotechnology, Beijing Forestry University, Beijing, 10083, China.
    Background: Auxin response factors (ARFs) in auxin signaling pathway are an important component that can regulate the transcription of auxin-responsive genes involved in almost all aspects of plant growth and development. To our knowledge, the comprehensive and systematic characterization of ARF genes has never been reported in Prunus sibirica, a novel woody biodiesel feedstock in China.

    Results: In this study, we identified 14 PsARF genes with a perfect open reading frame (ORF) in P. Read More

    Genetic diversity of Orf virus isolated from sheep in Uruguay.
    Arch Virol 2018 Jan 24. Epub 2018 Jan 24.
    Sección Virología, Facultad de Ciencias, UdelaR, Iguá 4225, 11400, Montevideo, Uruguay.
    Orf virus (ORFV) is the etiological agent of contagious ecthyma, a disease widely spread in the world that occasionally causes zoonotic infections. This work is the first molecular characterization of ORFV in Uruguay, where we analyzed twenty-one sheep samples, eighteen of which were recovered from thirteen ORFV outbreaks that occurred during 2004 to 2011 as well as three strains from a national vaccine. Phylogenetic analysis and the derived amino acid sequences from the B2L gene suggest that the Uruguayan virus do not form a unique cluster, with most of them displaying similarities with worldwide ORFV isolates as well as our vaccine strains. Read More

    Molecular characterization and functional activity of CXCL8_L3 in large yellow croaker Larimichthys crocea.
    Fish Shellfish Immunol 2018 Jan 31;75:124-131. Epub 2018 Jan 31.
    College of Animal Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, PR China; Key Laboratory of Marine Biogenetic Resources, Third Institute of Oceanography, State Oceanic Administration, Xiamen 361005, PR China; Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266071, PR China. Electronic address:
    CXCL8, also called interleukin-8, is a typical CXC chemokine that plays a key role in promoting inflammation. Phylogenetically, fish CXCL8 chemokines can be divided into three subgroups, CXCL8_L1, CXCL8_L2, and CXCL8_L3, of which CXCL8_L3 is a new subgroup. The CXCL8_L3 gene sequences have been reported in many fish species, but their function remains unknown. Read More

    The Definition of Open Reading Frame Revisited.
    Trends Genet 2018 Jan 20. Epub 2018 Jan 20.
    Department of Bioinformatics, Friedrich Schiller University Jena, Ernst-Abbe-Platz 2, 07743 Jena, Germany. Electronic address:
    The term open reading frame (ORF) is of central importance to gene finding. Surprisingly, at least three definitions are in use. We discuss several molecular biological and bioinformatics aspects, and we recommend using the definition in which an ORF is bounded by stop codons. Read More

    Ribosome profiling of the retrovirus murine leukemia virus.
    Retrovirology 2018 Jan 22;15(1):10. Epub 2018 Jan 22.
    Division of Virology, Department of Pathology, University of Cambridge, Tennis Court Rd, Cambridge, CB2 1QP, UK.
    Background: The retrovirus murine leukemia virus (MuLV) has an 8.3 kb RNA genome with a simple 5'-gag-pol-env-3' architecture. Translation of the pol gene is dependent upon readthrough of the gag UAG stop codon; whereas the env gene is translated from spliced mRNA transcripts. Read More

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