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    Scavenger receptor B promotes bacteria clearance by enhancing phagocytosis and attenuates white spot syndrome virus proliferation in Scylla paramamosian.
    Fish Shellfish Immunol 2018 Apr 18. Epub 2018 Apr 18.
    Guangdong Provincial Key Laboratory of Marine Biology, Shantou University, Shantou, 515063, China; Marine Biology Institute, Shantou University, Shantou, 515063, China. Electronic address:
    Phagocytosis and apoptosis are key cellular innate immune responses against bacteria and virus in invertebrates. Class B scavenger receptors (SRBs), which contain a CD36 domain, are critical pattern recognition receptors (PRRs) of phagocytosis for bacteria and apoptotic cells. In the present study, we identified a member of SRB subfamily in mud crab Scylla paramamosain, named Sp-SRB. Read More

    Molecular characterization of cu/Zn SOD gene in Asian clam Corbicula fluminea and mRNA expression and enzymatic activity modulation induced by metals.
    Gene 2018 Apr 18. Epub 2018 Apr 18.
    Science Center of Laboratory Animal, Nanchang University, 71 Yangming Road, Nanchang, 330003, China.
    The copper/zinc superoxide dismutase (Cu/Zn SOD) could effectively eliminate reactive oxygen species (ROS). In this study, the cDNA sequence of Cu/Zn SOD from Corbicula fluminea (designated as CfCu/Zn SOD) was cloned by the rapid amplification of cDNA ends (RACE) approaches. The full-length cDNA of CfCu/Zn SOD was of 1288 bp, including a 465 bp ORF encoding a protein of 154 amino acids. Read More

    [Isolation and expression analysis of a hexose transporter gene DoHT1 in Dendrobium officinale].
    Zhongguo Zhong Yao Za Zhi 2018 Mar;43(6):1124-1130
    Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100193, China.
    In this study, RACE technology was employed to isolate the full length cDNA of DoHT1 in Dendrobium officinale, followed by bioinformatics analysis of the sequence characteristics. And the expression pattern of the gene was also analyzed by quantitative PCR. The full length cDNA of DoHT1 was 1 586 bp in length, containing a 1 536 bp ORF, which encoded a 511-aa protein with molecular weight of 56. Read More

    Biochemical properties of a bacterially-expressed Bowman-Birk inhibitor from Rhynchosia sublobata (Schumach.) Meikle seeds and its activity against gut proteases of Achaea janata.
    Phytochemistry 2018 Apr 16;151:78-90. Epub 2018 Apr 16.
    Department of Biotechnology & Bioinformatics, School of Life Sciences, University of Hyderabad, Hyderabad 500 046, India. Electronic address:
    Crude proteinase inhibitors (CPIs) extracted from the seeds of Rhynchosia sublobata, a wild relative of pigeon pea showed pronounced inhibitory activity on the larval gut trypsin-like proteases of lepidopteran insect pest - Achaea janata. Consequently, a full-length cDNA of Bowman-Birk inhibitor gene (RsBBI1) was cloned from the immature seeds of R. sublobata. Read More

    The Conserved Proline18 in the Polerovirus P3a Is Important for Brassica Yellows Virus Systemic Infection.
    Front Microbiol 2018 4;9:613. Epub 2018 Apr 4.
    State Key Laboratory for Agrobiotechnology-Ministry of Agriculture Key Laboratory of Pest Monitoring and Green Management, China Agricultural University, Beijing, China.
    ORF3a, a newly identified non-AUG-initiated ORF encoded by members of genera and , is required for long-distance movement in plants. However, the mechanism of action of P3a in viral systemic movement is still not clear. In this study, sequencing of a brassica yellows virus (BrYV) mutant defective in systemic infection revealed two-nucleotide variation at positions 3406 and 3467 in the genome. Read More

    Genetic characterization of a novel picorna-like virus in Culex spp. mosquitoes from Mozambique.
    Virol J 2018 Apr 18;15(1):71. Epub 2018 Apr 18.
    Section of Virology, Department of Biomedical and Veterinary Public Health, Box 7028, Swedish University of Agricultural Sciences (SLU), 75007, Uppsala, Sweden.
    Background: Mosquitoes are the potential vectors for a variety of viruses that can cause diseases in the human and animal populations. Viruses in the order Picornavirales infect a broad range of hosts, including mosquitoes. In this study, we aimed to characterize a novel picorna-like virus from the Culex spp. Read More

    A novel mitochondrial orf147 causes cytoplasmic male sterility in pigeonpea by modulating aberrant anther dehiscence.
    Plant Mol Biol 2018 Apr 17. Epub 2018 Apr 17.
    International Crops Research Institute for the Semi-Arid Tropics (ICRISAT), Patancheru, Hyderabad, Telangana, 502324, India.
    Key Message: A novel open reading frame (ORF) identified and cloned from the A4 cytoplasm of Cajanus cajanifolius induced partial to complete male sterility when introduced into Arabidopsis and tobacco. Pigeonpea (Cajanus cajan L. Millsp. Read More

    Amsacta moorei entomopoxvirus encodes a functional heparin-binding glycosyltransferase (AMV248).
    Virus Genes 2018 Apr 17. Epub 2018 Apr 17.
    Department of Biology, Faculty of Sciences, Karadeniz Technical University, Trabzon, Turkey.
    Amsacta moorei entomopoxvirus (AMEV) infects certain lepidopteran and orthopteran insects and is the most studied member of the genus Betaentomopoxvirus. It has been considered as a potential vector for gene therapy, a vector to express exogenous proteins and a biological control agent. One of its open reading frames, amv248, encodes a putative glycosyltransferase and is the only known attachment protein conserved in AMEV and chordopoxviruses. Read More

    First report of prion-related protein gene () polymorphisms in cattle.
    Vet Rec 2018 Apr 17. Epub 2018 Apr 17.
    Department of Bioactive Material Sciences, Chonbuk National University, Jeonju, Jeonbuk, Republicof Korea.
    Prion diseases are caused by structural changes in normal prion protein (PrP). The prion gene family includes four members: prion protein (), prion-like protein (), shadow of () and prion-related protein (). Genetic association studies of prion diseases and the other genes in the prion gene family, except for , have been performed in cattle. Read More

    Molecular cloning and characterization of C tetrahydrofolate (C-THF) synthase in Bombyx mori, silkworm.
    Gene 2018 Apr 13. Epub 2018 Apr 13.
    School of Basic Medicine and Biological Sciences, Soochow University, Suzhou, Jiangsu 215123, PR China; National Engineering Laboratory for Modern Silk, Soochow University, Suzhou, Jiangsu 215123, PR China. Electronic address:
    Folate metabolism pathway is mainly associated with syntheses of nucleic acids and proteins as well as DNA methylation and repair. Cytoplasmic C tetrahydrofolate (C-THF) synthase is a central enzyme in folate metabolism and plays an important role in C-THF conversion. The full-length sequence of silkworm (Bombyx mori) cytoplasmic C-THF synthase (BmC-THF synthase) gene including an open reading frame (ORF) of 932 amino acid residues was cloned. Read More

    Transcription of blunt snout bream (Megalobrama amblycephala) HIF3α and its localization in the nucleus under both normoxic and hypoxic conditions.
    Biochem Biophys Res Commun 2018 Apr 18. Epub 2018 Apr 18.
    Genetics and Breeding Center for Blunt Snout Bream, Key Laboratory of Freshwater Aquatic Genetic Resources, Ministry of Agriculture, Shanghai Ocean University, Huchenghuan Road 999, Shanghai, 201306, China. Electronic address:
    Although hypoxia-inducible factor (HIF) 1α and 2α function as master regulators of the transcriptional response to hypoxia, the function of HIF3α and its responses to hypoxic stress remain unclear in teleost fish. Here, we characterized the HIF3α cDNA in hypoxia-sensitive blunt snout bream (Megalobrama amblycephala), with 3059 bp length, consisting of an open reading frame (ORF) encoding 643 amino acid residues. Blunt snout bream HIF3α mRNA was stably expressed during stages of embryonic development and in adult tissues. Read More

    Adapting 3' Rapid Amplification of CDNA Ends to Map Transcripts in Cancer.
    J Vis Exp 2018 Mar 28(133). Epub 2018 Mar 28.
    Department of Pharmaceutical Sciences, College of Pharmacy and Health Sciences, Butler University.
    Maturation of eukaryotic mRNAs involves 3' end formation, which involves the addition of a poly(A) tail. In order to map the 3' end of a gene, the traditional method of choice is 3' rapid amplification of cDNA ends (3' RACE). Protocols for 3' RACE require the careful design and selection of nested primers within the 3' untranslated region (3' UTR) of the target gene of interest. Read More

    Comparative sequence and structural analysis of Indian orf viruses based on major envelope immune-dominant protein (F1L), an homologue of pox viral p35/H3 protein.
    Gene 2018 Apr 12. Epub 2018 Apr 12.
    Pox Virus Laboratory, Division of Virology, ICAR-Indian Veterinary Research Institute, Mukteswar, 263138, Nainital (District), Uttarakhand, India.
    Orf virus (ORFV), a member of the genus Parapoxvirus in the family Poxviridae, is the cause of orf, a highly contagious zoonotic viral disease that affects mainly sheep and goats. In the present study, the sequence and phylogenetic analysis of Indian ORFV isolates (n = 15) from natural outbreaks in sheep and goats belonging to different geographical regions were analysed on the basis of F1L gene along with homology modelling of F1L protein. Multiple sequence alignments revealed highly conserved C-terminus and heterogeneity of N-terminus region of F1L among all orf viruses studied. Read More

    Hungarian tick-borne encephalitis viruses isolated from a 0.5-ha focus are closely related to Finnish strains.
    Ticks Tick Borne Dis 2018 Apr 7. Epub 2018 Apr 7.
    Molecular Biology Department, National Food Chain Safety Office, Veterinary Diagnostic Institute, Budapest, Hungary.
    Four tick-borne encephalitis virus strains were isolated from a small 0.5-ha focus over a six-year-long period (2011-2016) in Hungary. Two strains with identical genomes were isolated from Ixodes ricinus and Haemaphysalis concinna two months apart, which shows that the virus had not evolved separately in these tick species. Read More

    The evolution of subtype B HIV-1 tat in the Netherlands during 1985-2012.
    Virus Res 2018 Apr 11;250:51-64. Epub 2018 Apr 11.
    Laboratory of Experimental Virology, Department of Medical Microbiology, Academic Medical Center, University of Amsterdam, Meibergdreef 15, 1105 AZ, Amsterdam, The Netherlands.
    For the production of viral genomic RNA, HIV-1 is dependent on an early viral protein, Tat, which is required for high-level transcription. The quantity of viral RNA detectable in blood of HIV-1 infected individuals varies dramatically, and a factor involved could be the efficiency of Tat protein variants to stimulate RNA transcription. HIV-1 virulence, measured by set-point viral load, has been observed to increase over time in the Netherlands and elsewhere. Read More

    Assessment of area spread of porcine reproductive and respiratory syndrome (PRRS) virus in three clusters of swine farms.
    Transbound Emerg Dis 2018 Apr 14. Epub 2018 Apr 14.
    Department of Population Medicine, University of Minnesota, St Paul, MN, USA.
    Despite decades of porcine reproductive and respiratory syndrome (PRRS) research, outbreaks with emerging and re-emerging PRRS virus (PRRSV) strains are not uncommon in North America. The role of area spread, commonly referred but not limited to airborne transmission, in originating such outbreaks is currently unknown. The main objective of this study was to explore the role of area spread on the occurrence of new PRRSV cases by combining information on genetic similarity among recovered PRRSV isolate's open-reading frame (ORF) 5 sequences and publicly available weather data. Read More

    Molecular cloning, expression and biochemical characterization of hemoglobin gene from ark shell Scapharca broughtonii.
    Fish Shellfish Immunol 2018 Apr 9. Epub 2018 Apr 9.
    Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, PR China; National Demonstration Center for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai 201306, PR China.
    Hemoglobin, the main component of haemolymph, is widely distributed in animals. Although its important oxygen transport functions has been extensively reported, studies on the immune function of hemoglobin in mollusc are few. Research on immune of hemoglobin of ark shell Scapharca broughtonii attracted more and more attention due to its ownership of erythrocyte comparing with many other shellfish. Read More

    Bioinformatics characterization of a cathepsin B transcript from the giant river prawn, Macrobrachium rosenbergii: Homology modeling and expression analysis after Aeromonas hydrophila infection.
    Comp Biochem Physiol B Biochem Mol Biol 2018 Apr 10;221-222:18-28. Epub 2018 Apr 10.
    Department of Aquaculture, Faculty of Fisheries, Kasetsart University, 50 Ngamwongwan Road, Bangkok 10900, Thailand. Electronic address:
    Cathepsin B is a lysosomal proteolytic enzyme that has been suggested to play a role in pathological processes of immune system. In this study, the full-length cDNA sequence of cathepsin B transcript in the giant river prawn Macrobrachium rosenbergii (MrCTSB) was obtained from 454 pyrosequencing of cDNAs from hepatopancreas and muscle. It was 1158 bp in length, containing an open reading frame (ORF) of 987 bp corresponding to 328 amino acids. Read More

    Molecular characterization of the lipophorin receptor in the crustacean ectoparasite Lepeophtheirus salmonis.
    PLoS One 2018 12;13(4):e0195783. Epub 2018 Apr 12.
    Computational Biology Unit, Department of Biological Sciences, University of Bergen, Bergen, Norway.
    The Salmon louse (Lepeophtheirus salmonis) is a marine ectoparasite of salmonid fish in the Northern Hemisphere and considered as a major challenge in aquaculture and a threat to wild populations of salmonids. Adult female lice produce a large number of lipid-rich eggs, however, the mechanism of maternal lipid transport into developing eggs during salmon louse reproduction has not been described. In the present study, a full-length L. Read More

    Molecular Cloning and Functional Characterization of a Dihydroflavonol 4-Reductase from Vitis bellula.
    Molecules 2018 Apr 10;23(4). Epub 2018 Apr 10.
    Hunan Provincial Key Laboratory of Plant Resources Conservation and Utilization, College of Biology and Environmental Sciences, Jishou University, No. 120 Ren Min Nan Lu, Jishou City 416000, China.
    is a new grape crop in southern China. Berries of this species are rich in antioxidative anthocyanins and proanthocyanidins. This study reports cloning and functional characterization of a cDNA encoding a dihydroflavonol reductase (VbDFR) involved in the biosynthesis of anthocyanins and proanthocyanidins. Read More

    Parapoxvirus Infections in the Country of Georgia: A Case Series.
    Am J Trop Med Hyg 2018 Apr 9. Epub 2018 Apr 9.
    University of Michigan, Ann Arbor, Michigan.
    Infections caused by viruses of the parapoxvirus (PPV) genus, including orf and pseudocowpox viruses, are frequently seen in both humans and animals in many regions of the world. These infections are often misdiagnosed or neglected because of the lack of clinician awareness, inadequate diagnostic capacity, and their relatively mild disease presentation, which may result in affected individuals not seeking medical attention. Although PPV infections should be routinely considered in patients with cutaneous lesions, especially in those who have occupational exposure to farm animals, they are often excluded from the differential diagnosis because they are not perceived as serious, resulting in underestimation of the burden of disease. Read More

    Underdiagnosed and disfiguring - Radiation-induced morphea following breast cancer treatment.
    Breast 2018 Apr 7;39:97-100. Epub 2018 Apr 7.
    Department of Dermatology, Tel-Aviv Sourasky Medical Center, Affiliated with the Sackler Faculty of Medicine, Tel-Aviv University, Tel-Aviv, Israel.
    Objectives: Radiation-induced morphea (RIM) is a rare, painful and disfiguring complication in women who receive adjuvant radiotherapy treatment for breast cancer. It is a distinct entity unrelated to radiation-induced fibrosis of the breast.

    Materials And Methods: Between the years 2005-2016, approximately 12,000 breast cancer cases were treated with surgery and radiation in the central district of Israel. Read More

    Bovine herpes virus type-4 infection among postpartum dairy cows in California: risk factors and phylogenetic analysis.
    Epidemiol Infect 2018 Apr 10:1-9. Epub 2018 Apr 10.
    California Animal Health and Food Safety Laboratory System,University of California,School of Veterinary Medicine,Thurman Lab,Davis,CA 95616,USA.
    The aims of this study were to determine the prevalence and associated risk factors of bovine herpes virus type-4 (BoHV-4) infection and describe the genetic characteristics and predominant genotypes of the virus in Yolo and Tulare counties, California. A cross-sectional study involving multi-stage sampling technique was used. One hundred and forty-eight post-partum cows were enrolled from 11 dairy farms. Read More

    A Stress Response that Monitors and Regulates mRNA Structure Is Central to Cold Shock Adaptation.
    Mol Cell 2018 Apr 5;70(2):274-286.e7. Epub 2018 Apr 5.
    Department of Microbiology and Immunology, University of California, San Francisco, San Francisco, CA 94158, USA; Department of Cell and Tissue Biology, University of California, San Francisco, San Francisco, CA 94158, USA; California Institute of Quantitative Biology, University of California, San Francisco, San Francisco, CA 94158, USA. Electronic address:
    Temperature influences the structural and functional properties of cellular components, necessitating stress responses to restore homeostasis following temperature shift. Whereas the circuitry controlling the heat shock response is well understood, that controlling the E. coli cold shock adaptation program is not. Read More

    A novel fucolectin from Apostichopus japonicus with broad PAMP recognition pattern.
    Fish Shellfish Immunol 2018 Apr 5;77:402-409. Epub 2018 Apr 5.
    Liaoning Key Laboratory of Marine Animal Immunology and Disease Control, Dalian Ocean University, Dalian, 116023, China; Laboratory of Marine Fisheries Science and Food Production Process, Qingdao National Laboratory for Marine Science and Technology, Qingdao, 266071, China. Electronic address:
    F-type lectin (also known as fucolectin) is a newly identified family of fucose binding lectins with the sequence characters of a fucose binding motif and a unique lectin fold (the "F-type" fold). In the present study, a fucolectin was identified from sea cucumber Apostichopus japonicus (designated AjFL-1). The open reading frame (ORF) of AjFL-1 was of 546 bp, encoding a polypeptide of 181 amino acids with a predicted molecular mass of about 20 kDa. Read More

    NmtA, a novel metallothionein of Anabaena sp. strain PCC 7120 imparts protection against cadmium stress but not oxidative stress.
    Aquat Toxicol 2018 Mar 30;199:152-161. Epub 2018 Mar 30.
    Molecular Biology Division, Bhabha Atomic Research Centre, Trombay, Mumbai, 400085, India; Homi Bhabha National Institute, Anushakti Nagar, Mumbai, 400094, India. Electronic address:
    Metallothioneins (MTs) are low molecular weight, sulfhydryl-containing, cysteine-rich, metal-binding proteins. Eukaryotes have multiple metallothionein genes; however, there is dearth of reports on prokaryotic metallothioneins. Bacterial MTs with SmtA from Synechococcus PCC 7942 as prototype have been studied in the context of cadmium detoxification. Read More

    Molecular characterization and expression patterns of ghrelin in the reindeer (Rangifer tarandus).
    Pol J Vet Sci 2018 Mar;21(1):55-64
    Veterinary Medicine College of the Inner Mongolia Agricultural University, Hohhot, 010018, People's Republic of China.
    Ghrelin is a novel growth hormone (GH)-releasing peptide, which has been identified as an endogenous ligand for the GH-sretagogue receptor. The sequence and expression of ghrelin has been determined in many species. In this study, to reveal the molecular characterization and expression patterns of ghrelin in the reindeer (Rangifer tarandus), the full-length DNA and cDNA encoding ghrelin were cloned from reindeer stomach using genome walking and rapid amplification of complementary deoxyribonucleic acid ends (RACE). Read More

    A Gossypium hirsutum GDSL lipase/hydrolase gene (GhGLIP) appears to be involved in promoting seed growth in Arabidopsis.
    PLoS One 2018 5;13(4):e0195556. Epub 2018 Apr 5.
    College of Life Sciences, Key Laboratory of Agrobiotechnolog, Shihezi University, Shihezi, Xinjiang, China.
    GDSL lipase (GLIP) plays a pivotal role in plant cell growth as a multifunctional hydrolytic enzyme. Herein, a cotton (Gossypium hirsutum L. cv Xuzhou 142) GDSL lipase gene (GhGLIP) was obtained from developing ovules and fibers. Read More

    Genomewide characterisation of the genetic diversity of carotenogenesis in bacteria of the order Sphingomonadales.
    Microb Genom 2018 Apr 5. Epub 2018 Apr 5.
    1​Institute of Bioinformatics and Applied Biotechnology, Biotech Park, Electronic City, Bengaluru 560100, Karnataka, India.
    The order Sphingomonadales is a taxon of bacteria with a variety of physiological features and carotenoid pigments. Some of the coloured strains within this order are known to be aerobic anoxygenic phototrophs that contain characteristic photosynthesis gene clusters (PGCs). Previous work has shown that majority of the ORFs putatively involved in the biosynthesis of C40 carotenoids are located outside the PGCs in these strains. Read More

    A Prokineticin (PK)-like cytokine from Chinese mitten crab Eriocheir sinensis promotes the production of hemocytes via reactive oxygen species.
    Fish Shellfish Immunol 2018 Mar 30. Epub 2018 Mar 30.
    Functional Laboratory of Marine Fisheries Science and Food Production Process, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266071, China; Liaoning Key Laboratory of Marine Animal Immunology, Dalian Ocean University, Dalian 116023, China. Electronic address:
    Astakine is a cytokine-like factor containing a prokineticin domain, which directly participates in hematopoiesis and blood cell differentiation. In the present study, a novel Astakine gene was identified from Chinese mitten crab Eriocheir sinensis (designated as EsAst). The full-length cDNA of EsAst was of 1163 bp, consisting of a 5' untranslated region (UTR) of 120 bp, a 3' UTR of 656 bp, and an open reading frame (ORF) of 387 bp encoding a polypeptide of 128 amino acids. Read More

    Identification, characterization and expression analysis of MAVS in Pelodiscus sinensis after challenge with Poly I:C.
    Fish Shellfish Immunol 2018 Mar 30;77:222-232. Epub 2018 Mar 30.
    College of Animal Sciences, Zhejiang University, 866 Yuhangtang Road, Hangzhou 310058, Zhejiang Province, China.
    Pelodiscus sinensis, which is one of the important reptile species in the aquaculture industry in China, frequently suffers from serious infectious diseases caused by viruses. However, there is a lack of biological knowledge about its antiviral innate immunity. In this study, we identified and characterized the open reading frame (ORF) of PsMAVS cDNA in P. Read More

    Description of a catalase gene involved in responses to temperature stress.
    PeerJ 2018 26;6:e4532. Epub 2018 Mar 26.
    College of Marine Life Science, Ocean University of China, Qingdao, China.
    Catalase (CAT) is an antioxidant enzyme that plays a significant role in cellular protection against oxidative damage by degradation of hydrogen peroxide to oxygen and water. In the present study, the complete CAT cDNA sequence of was identified through expressed sequence tags (EST) analysis and the rapid amplification of cDNA ends (RACE) technique. The nucleotide sequence of CAT cDNA consisted of 1,816 bp with a 1,434 bp open reading frame (ORF), encoding a polypeptide of 477 amino acid residues, which possessed significant homology to other known plant CATs. Read More

    Genetic mapping of a lobed-leaf gene associated with salt tolerance in Brassica napus L.
    Plant Sci 2018 Apr 31;269:75-84. Epub 2018 Jan 31.
    State Key Laboratory of Crop Stress Biology for Arid Areas/College of Agronomy, Northwest A&F University, Yangling, Shaanxi, 712100, China. Electronic address:
    Lobed leaf is a common trait, which is related with photosynthesis and plant stress resistance in crops. In order to fine map and isolate the lobed-leaf gene in Brassica napus, an F population derived from 2205 (salt tolerance) and 1423 (salt sensitive) was constructed, and the quantitative trait locus (QTL) technology was adopted to identify the QTLs related to lobed leaf formation. As a result, one major QTL was identified on LG10, and two intron polymorphic (IP) markers and one sequence characterized amplified region (SCAR) marker were successfully developed in QTL region. Read More

    The L83L ORF of African swine fever virus strain Georgia encodes for a non-essential gene that interacts with the host protein IL-1β.
    Virus Res 2018 Mar 29;249:116-123. Epub 2018 Mar 29.
    Agricultural Research Service and Plum Island Animal Disease Center, Greenport, NY 11944, USA. Electronic address:
    African swine fever virus (ASFV) causes a contagious and frequently lethal disease of pigs causing significant economic consequences to the swine industry. The ASFV genome encodes for more than 150 genes, but only a few of them have been studied in detail. Here we report the characterization of open reading frame L83L which encodes a highly conserved protein across all ASFV isolates. Read More

    Identification of host cellular proteins LAGE3 and IGFBP6 that interact with orf virus protein ORFV024.
    Gene 2018 Mar 29. Epub 2018 Mar 29.
    Institute of Antibody Engineering, School of Laboratory Medicine and Biotechnology, Southern Medical University, Guangzhou 510515, China; Department of Laboratory Medicine, School of Stomatology and Medicine, Foshan University, 5 Hebin Road, Chancheng District, Foshan, Guangdong Province 528000, China. Electronic address:
    Objective: Orf virus (ORFV) is the pathogen causing contagious pustular dermatitis in goats, sheep and herdsmen. Evidence has confirmed that ORFV can be used as a preventive and therapeutic immunomodulatory agent in several animal models. Our previous data demonstrated that ORFV024 is able to inhibit activation of the NF-κB signaling pathway and act as an important modulator for early immune responses against viral infection. Read More

    Protective immunity against Eimeria maxima induced by vaccines of Em14-3-3 antigen.
    Vet Parasitol 2018 Apr 11;253:79-86. Epub 2018 Feb 11.
    College of Veterinary Medicine, Nanjing Agriculture University, 1 Weigang, Nanjing, Jiangsu 210095, PR China. Electronic address:
    Eimeria maxima 14-3-3 (Em14-3-3) open reading frame (ORF) which consisted of 861 bp encoding a protein of 286 amino acids was successfully amplified and sequenced. Subsequently, the Em14-3-3 ORF was subcloned into pET-32a (+) and pVAX1, respectively. RT-PCR and immunoblot analyses confirmed that the target gene was successfully transcribed and expressed in vivo. Read More

    Near shot-noise limited time-resolved circular dichroism pump-probe spectrometer.
    Rev Sci Instrum 2018 Mar;89(3):033104
    Department of Physics and Astronomy, Purdue University, West Lafayette, Indiana 47906, USA.
    We describe an optical near shot-noise limited time-resolved circular dichroism (TRCD) pump-probe spectrometer capable of reliably measuring circular dichroism signals in the order of μdeg with nanosecond time resolution. Such sensitivity is achieved through a modification of existing TRCD designs and introduction of a new data processing protocol that eliminates approximations that have caused substantial nonlinearities in past measurements and allows the measurement of absorption and circular dichroism transients simultaneously with a single pump pulse. The exceptional signal-to-noise ratio of the described setup makes the TRCD technique applicable to a large range of non-biological and biological systems. Read More

    Evolution of photosynthetic reaction centers: insights from the structure of the heliobacterial reaction center.
    Photosynth Res 2018 Mar 30. Epub 2018 Mar 30.
    School of Molecular Sciences, Arizona State University, Tempe, AZ, 85287, USA.
    The proliferation of phototrophy within early-branching prokaryotes represented a significant step forward in metabolic evolution. All available evidence supports the hypothesis that the photosynthetic reaction center (RC)-the pigment-protein complex in which electromagnetic energy (i.e. Read More

    Cloning and functional characterization of thioredoxin genes from large yellow croaker Larimichthys crocea.
    Fish Shellfish Immunol 2018 Mar 28;77:385-391. Epub 2018 Mar 28.
    National Engineering Research Center of Marine Facilities Aquaculture, Zhejiang Ocean University, Zhoushan, 316022, PR China.
    Thioredoxin(Trx)with a redox-active disulfide/dithiol in the active site, is an ubiquitous disulfide reductase majorly responsible for maintaining the balance of reactive oxygen species. In this study, the complete thioredoxin-like protein 1 (designated as LcTrx) was cloned from large yellow croaker Larimichthys crocea through rapid amplification of cDNA ends. The full-length cDNA of LcTrx was 1295 bp in length containing a 131 bp 5' untranslated region (UTR) ,a 3'UTR of 294bp with a poly (A) tail, and an 870 bp open reading frame (ORF) encoding a polypeptide of 289 amino acids. Read More

    [Clone and expression of CpGAPDH gene in Codonopsis pilosula].
    Zhongguo Zhong Yao Za Zhi 2018 Feb;43(4):712-720
    College of Pharmacy, Shanxi Medical University, Taiyuan 030001, China.
    GAPDH(glyceraldehyde-3-phosphate dehydrogenase) gene is a key enzyme gene in carbohydrate metabolism and always used as reference gene. To clarify and complete the biosynthetic pathway of polysaccharide, the GAPDH gene in Codonopsis pilosula, named CpGAPDH, was cloned according to the transcriptome of pilosula, using the GAPDH gene in potato as query. The CpGAPDH contained a 1 014 bp open reading frame(ORF) and encoded a protein with 337 amino acids. Read More

    [Gene cloning and prokaryotic expression of glycosyltransferase from Ligustrum quihoui].
    Zhongguo Zhong Yao Za Zhi 2018 Feb;43(4):704-711
    Biological Engineering Laboratory, College of Pharmacy, Hunan University of Chinese Medicine, Changsha 410208, China.
    According to the previous results from transcriptome analysis of Ligustrum quihoui, a glycosyltransferase gene(xynzUGT) was cloned by rapid amplification of cDNA ends(RACE). The full length cDNA of xynzUGT was 1 598 bp, consisting of 66 bp 5'-UTR, 1 440 bp ORF and 92 bp 3'-UTR. The ORF encoded a 480 amino-acid protein(xynzUGT) with a molecular weight of 54 826. Read More

    Simian Varicella Virus Infects Enteric Neurons and α4β7 Integrin-Expressing Gut-Tropic T-Cells in Nonhuman Primates.
    Viruses 2018 03 28;10(4). Epub 2018 Mar 28.
    Department of Viroscience, Erasmus MC, 3015 CE Rotterdam, The Netherlands.
    The pathogenesis of enteric zoster, a rare debilitating complication of reactivation of latent varicella-zoster virus (VZV) in the enteric nervous system (ENS), is largely unknown. Infection of monkeys with the closely related simian varicella virus (SVV) mimics VZV disease in humans. In this study, we determined the applicability of the SVV nonhuman primate model to study infection of the ENS. Read More

    Hepatitis E virus in feral rabbits along a rural-urban transect in Central Germany.
    Infect Genet Evol 2018 Mar 26;61:155-159. Epub 2018 Mar 26.
    Friedrich-Loeffler-Institut, Institute of Novel and Emerging Infectious Diseases, Greifswald-Insel Riems, Germany; German Center for Infection Research (DZIF), Partner Site Hamburg-Luebeck-Borstel-Insel Riems, Germany. Electronic address:
    Rabbit associated genotype 3 hepatitis E virus (HEV) strains were detected in feral, pet and farm rabbits in different parts of the world since 2009 and recently also in human patients. Here, we report a serological and molecular survey on 72 feral rabbits, collected along a rural-urban transect in and next to Frankfurt am Main, Central Germany. ELISA investigations revealed in 25 of 72 (34. Read More

    VZV ORF63 protects human neuronal and keratinocyte cell lines from apoptosis and changes its localization upon apoptosis induction.
    J Virol 2018 Mar 28. Epub 2018 Mar 28.
    Discipline of Infectious Diseases and Immunology, The University of Sydney, Camperdown, New South Wales 2050, Australia.
    There are many facets of varicella zoster virus (VZV) pathogenesis that are not fully understood, such as the mechanisms involved in the establishment of lifelong latency, reactivation and development of serious conditions such as post herpetic neuralgia (PHN). Virus-encoded modulation of apoptosis has been suggested to play an important role in these processes. VZV open reading frame (ORF) 63 has been shown to modulate apoptosis in a cell type specific manner, but the impact of ORF63 on cell death pathways has not been examined in isolation, in the context of human cells. Read More

    ICTV Virus Taxonomy Profile: Hypoviridae.
    J Gen Virol 2018 Mar 28. Epub 2018 Mar 28.
    11​Department of Plant Biology and Pathology, Rutgers University, New Brunswick, NJ 08901, USA.
    The Hypoviridae, comprising one genus, Hypovirus, is a family of capsidless viruses with positive-sense, ssRNA genomes of 9.1-12.7 kb that possess either a single large ORF or two ORFs. Read More

    [Occupational risk factors and perceptions of air pollution by motorcycle taxi drivers in Cotonou, Benin].
    Sante Publique 2018 Jan Feb;30(1):125-134
    Introduction: Motorcycle taxis occupy a major place in urban transport. Motorcycle taxi drivers are exposed to occupational risk factors (ORF) including air pollution. The objective of this study was to evaluate these ORF among motorcycle taxi drivers and their perceptions of air pollution. Read More

    Adeno-associated virus Rep proteins antagonize phosphatase PP1 to counteract KAP1 repression of the latent viral genome.
    Proc Natl Acad Sci U S A 2018 Apr 26;115(15):E3529-E3538. Epub 2018 Mar 26.
    Department of Infectious Diseases, School of Immunology and Microbial Sciences, King's College London, SE1 9RT London, United Kingdom;
    Adeno-associated virus (AAV) is a small human whose low immunogenicity and capacity for long-term persistence have led to its widespread use as vector for gene therapy. Despite great recent successes in AAV-based gene therapy, further improvements in vector technology may be hindered by an inadequate understanding of various aspects of basic AAV biology. AAV is unique in that its replication is largely dependent on a helper virus and cellular factors. Read More

    Differential expression of two Piwil orthologs during embryonic and gonadal development in pufferfish, Takifugu fasciatus.
    Comp Biochem Physiol B Biochem Mol Biol 2018 May 24;219-220:44-51. Epub 2018 Mar 24.
    Key Laboratory of Biodiversity and Biotechnology of Jiangsu Province, College of Life Sciences, Nanjing Normal University, Nanjing 210023, China; Co-Innovation Center for Marine Bio-Industry Technology of Jiangsu Province, Lianyungang, Jiangsu 222005, China. Electronic address:
    Piwil was an important regulator gene in germ cell division during gonadal development. Two Piwi-like genes, Piwil1 and Piwil2, were first cloned from T. fasciatus. Read More

    A novel Babesia orientalis 135-kilodalton spherical body protein like: identification of its secretion into cytoplasm of infected erythrocytes.
    Parasit Vectors 2018 Mar 27;11(1):205. Epub 2018 Mar 27.
    State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, 430070, Hubei, China.
    Background: The spherical body is a distinct organelle only existing in Babesia and Theileria. Spherical body proteins (SBPs) are secreted from spherical bodies and incorporated into the cytoplasm of infected erythrocytes during invasion and post-invasion stages. Four different SBP homologues (SBP1, SBP2, SBP3 and SBP4) have been identified in Babesia bovis and Babesia bigemina. Read More

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