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    Deltabaculoviruses encode a functional type I budded virus envelope fusion protein.
    J Gen Virol 2017 Apr 28. Epub 2017 Apr 28.
    1​Laboratory of Virology, Wageningen University, Wageningen, The Netherlands.
    Envelope fusion proteins (F proteins) are major constituents of budded viruses (BVs) of alpha- and betabaculoviruses (Baculoviridae) and are essential for the systemic infection of insect larvae and insect cell culture. An f homologue gene is absent in gammabaculoviruses. Here we characterized the putative F-homologue (Cuni-F), encoded by (ORF) 104 of Culex nigripalpus nucleopolyhedrovirus (CuniNPV), the only deltabaculovirus member. Read More

    Genetic characterization of equid herpesvirus type 1 from cases of abortion in Poland.
    Arch Virol 2017 Apr 27. Epub 2017 Apr 27.
    Department of Virology, National Veterinary Research Institute, Al. Partyzantow 57, 24-100, Pulawy, Poland.
    Equid herpesvirus type 1 (EHV-1) is a common viral infection associated with varied clinical outcomes including respiratory disease, abortion and neurological disease. We have characterized EHV-1 sequences (n = 38) obtained from cases of equine abortion in Poland between 1999 and 2016, based on sequencing of PCR products from open reading frames (ORF) 30 and 68 of the EHV-1 genome. The majority (81. Read More

    Two ribosome recruitment sites direct multiple translation events within HIV1 Gag open reading frame.
    Nucleic Acids Res 2017 Apr 26. Epub 2017 Apr 26.
    CNRS UMR 8015, Laboratoire de cristallographie et RMN Biologiques, Université Paris Descartes, 4 avenue de l'Observatoire, 75270 Paris Cedex 06, France.
    In the late phase of the HIV virus cycle, the unspliced genomic RNA is exported to the cytoplasm for the necessary translation of the Gag and Gag-pol polyproteins. Three distinct translation initiation mechanisms ensuring Gag production have been described with little rationale for their multiplicity. The Gag-IRES has the singularity to be located within Gag ORF and to directly interact with ribosomal 40S. Read More

    Regulation and Molecular Basis of Environmental Muropeptide Uptake and Utilization in Fastidious Oral Anaerobe Tannerella forsythia.
    Front Microbiol 2017 12;8:648. Epub 2017 Apr 12.
    Department of Oral Biology, University at Buffalo, BuffaloNY, USA.
    Tannerella forsythia is a Gram-negative oral anaerobe associated with periodontitis. This bacterium is auxotrophic for the peptidoglycan amino sugar N-acetylmuramic (MurNAc) and likely relies on scavenging peptidoglycan fragments (muropeptides) released by cohabiting bacteria during their cell wall recycling. Many Gram-negative bacteria utilize an inner membrane permease, AmpG, to transport peptidoglycan fragments into their cytoplasm. Read More

    Model-enabled gene search (MEGS) allows fast and direct discovery of enzymatic and transport gene functions in the marine bacterium Vibrio fischeri.
    J Biol Chem 2017 Apr 26. Epub 2017 Apr 26.
    University of Wisconsin-Madison, United States;
    While genomes can be rapidly sequenced, the functions of many genes are incompletely or erroneously annotated because of a lack of experimental evidence or prior functional knowledge in sequence databases. To address this weakness, we describe here a model-enabled gene search (MEGS) approach that (i) identifies metabolic functions either missing from an organism's genome annotation or incorrectly assigned to an ORF, by using discrepancies between metabolic model predictions and experimental culturing data; (ii) designs functional selection experiments for these specific metabolic functions; and (iii) selects a candidate gene(s) responsible for these functions from a genomic library and directly interrogates this gene's function experimentally. To discover gene functions, MEGS uses genomic functional selections instead of relying on correlations across large experimental datasets or sequence similarity as other approaches do. Read More

    Detection of Serum Antibodies to Hepatitis E Virus Based on HEV Genotype 3 ORF2 Capsid Protein Expressed in Nicotiana benthamiana.
    Ann Lab Med 2017 Jul;37(4):313-319
    Department of Plant Physiology and Molecular Biology, University of Plovdiv "Paisii Hilendarski", Plovdiv, Bulgaria.
    Background: Hepatitis E virus (HEV) causes epidemics in developing countries and is primarily transmitted through the fecal-oral route. There have been recent reports on the zoonotic spread of the virus, and several animal species, primarily pigs, have been recognized as reservoirs of HEV. Because of its possible spread, there is an urgent need of a method for the cost-effective production of HEV proteins that can be used as diagnostic antigens for the serological detection of anti-HEV antibodies. Read More

    Molecular cloning, characterization and expression analysis of heat shock protein 90 in albino northern snakehead Channa argus.
    Gene 2017 Apr 22. Epub 2017 Apr 22.
    College of Marine Sciences, South China Agricultural University, Guangzhou 510642, Guangdong, China. Electronic address:
    The great albino northern snakehead Channa argus is habitual to only the Sichuan Jialing Rivers System in China, making its introduction difficult to other riverine systems. Here, we characterized heat shock protein 90 (AcaHSP90) and probed its molecular responses toward the environmental stressors that C. argus can face during its introduction and breeding in the other southern latitudes of China. Read More

    Codon Usage Optimization and Construction of Plasmid Encoding Iranian Human Papillomavirus Type 16 E7 Oncogene for Lactococcus Lactis Subsp. Cremoris MG1363
    Asian Pac J Cancer Prev 2017 03 1;18(3):783-788. Epub 2017 Mar 1.
    Department of Microbiology, Faculty of Basic Sciences, Science and Research Branch, Islamic Azad University, Tehran, IR Iran. Email:
    HPV 16 intratypic sequence variations has been recognized in association with oncogenic potential diverge and geographic distribution. This study aimed to investigate nucleotide modifications and optimization of HPV 16 E7 regions from Iranian infected women. Cervical biopsies from 79/163 HPV 16 positive cancer patients detected in our study were analyzed by PCR in a couple of cloning of a complete ORF of the E7 gene, and sequencing. Read More

    Apolipophorin-III Acts as a Positive Regulator of Plasmodium Development in Anopheles stephensi.
    Front Physiol 2017 7;8:185. Epub 2017 Apr 7.
    Molecular Parasitology and Vector Biology Laboratory, Department of Biological Sciences, Birla Institute of Technology and SciencePilani, India.
    Apolipophorin III (ApoLp-III) is a well-known hemolymph protein having a functional role in lipid transport and immune responses of insects. Here we report the molecular and functional characterization of Anopheles stephensi Apolipophorin-III (AsApoLp-III) gene. This gene consists of 679 nucleotides arranged into two exons of 45 and 540 bp that give an ORF encoding 194 amino acid residues. Read More

    High precision genome sequencing of engineered G. oxydans 621H by combining long nanopore and short accurate Illumina reads.
    J Biotechnol 2017 Apr 19. Epub 2017 Apr 19.
    Institute of Bio- and Geosciences IBG-1: Biotechnology, Forschungszentrum Jülich GmbH, 52425 Jülich, Germany; The Bioeconomy Science Center (BioSC), c/o Forschungszentrum Jülich GmbH, 52425 Jülich, Germany. Electronic address:
    State of the art and novel high-throughput DNA sequencing technologies enable fascinating opportunities and applications in the life sciences including microbial genomics. Short high-quality read data already enable not only microbial genome sequencing, yet can be inadequately to solve problems in genome assemblies and for the analysis of structural variants, especially in engineered microbial cell factories. Single-molecule real-time sequencing technologies generating long reads promise to solve such assembly problems. Read More

    Mitovirus UGA(Trp) codon usage parallels that of host mitochondria.
    Virology 2017 Apr 18;507:96-100. Epub 2017 Apr 18.
    Department of Microbiology & Immunobiology, Harvard Medical School, Boston, MA 02115, USA. Electronic address:
    Mitoviruses replicate in mitochondria of their host fungi. They have small RNA genomes that encompass a single ORF encoding the viral RdRp. Since UGA codons encode Trp in fungal mitochondria, the RdRp ORF of a typical mitovirus includes multiple UGA codons. Read More

    Cloning and Identification of a Novel Steroid 11α-hydroxylase Gene from Absidia coerulea.
    J Steroid Biochem Mol Biol 2017 Apr 17. Epub 2017 Apr 17.
    College of Chemical Engineering and Materials Science, Tianjin University of Science and Technology, Tianjin 300457, China. Electronic address:
    Steroid 11-hydroxylation by filamentous fungi is a major route for industrial scale production of key intermediates in the synthesis of steroid drugs. Although it is well established that enzymes involved in fungal hydroxylation of steroids are cytochrome P450s (CYP), few fungal steroid hydroxylase genes have been identified. In this study, we identified a novel 11α-hydroxylase gene CYP5311B1 from Absidia coerulea AS3. Read More

    Molecular cloning and characterization of kiss1 in Brandt's voles (Lasiopodomys brandtii).
    Comp Biochem Physiol B Biochem Mol Biol 2017 Apr 18;208-209:68-74. Epub 2017 Apr 18.
    Key Laboratory of Integrated Pest Management in Crops, Ministry of Agriculture, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, 2 Yuanmingyuanxi Road, Beijing 100193, PR China. Electronic address:
    Kisspeptin, encoded by kiss1, has been regarded as a major modulator of mammalian puberty and fertility due to its stimulation on GnRH. Brandt's vole is one of the main pest species on the Inner Mongolian steppes for its striking reproductive capacity and kiss1 is a key candidate gene related to reproductive regulatory cascades. In this study, kiss1 cDNA was cloned from the hypothalamus of Brandt's voles and kiss1 mRNA levels were investigated in different tissues, and at different developmental stages, using high-throughput real-time PCR. Read More

    Characterization and Genome Sequence of Marine Alteromonas gracilis Phage PB15 Isolated from the Yellow Sea, China.
    Curr Microbiol 2017 Apr 20. Epub 2017 Apr 20.
    College of Marine Life Sciences, Ocean University of China, Qingdao, 266003, China.
    A novel marine Alteromonas gracilis siphovirus, phage PB15, was isolated from the surface water of the Yellow Sea in August 2015. It has a head diameter of 58 ± 5 nm head and a contractile tail approximately 105 ± 10 nm in length, and overall, the morphology suggests that PB15 belongs to the family Siphoviridae. PB15 phage is stable at over the temperature range 0-60 °C. Read More

    Usefulness of the MRP2 promoter to overcome the chemoresistance of gastrointestinal and liver tumors by enhancing the expression of the drug transporter OATP1B1.
    Oncotarget 2017 Mar 11. Epub 2017 Mar 11.
    Experimental Hepatology and Drug Targeting (HEVEFARM), IBSAL, University of Salamanca, Salamanca, Spain.
    Tumor response to chemotherapy is often limited by drug export through ABC proteins. To overcome this problem, here we have investigated the usefulness of inducing the expression of the multidrug uptake transporter OATP1B1 under the control of the MRP2 promoter (MRP2pr). Human hepatoma cells (Alexander) were transfected with MRP2pr fragments of different length fused to the firefly luciferase ORF in order to select the shortest fragment with the highest response to dexamethasone, which was subsequently used to generate the chimeric construct MRP2pr-OATP1B1-V5. Read More

    Molecular identification and immunological characteristics of goose suppressor of cytokine signaling 1 (SOCS-1) in vitro and vivo following DTMUV challenge.
    Cytokine 2017 Apr 13. Epub 2017 Apr 13.
    Institute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu, Sichuan 611130, China; Avian Disease Research Center, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, Sichuan 611130, China; Key Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University, Chengdu, Sichuan 611130, China. Electronic address:
    Purpose suppressor of cytokine signaling 1 (SOCS-1) is inducible feedback inhibitors of cytokine signaling and involved in viral infection through regulation of both innate and adaptive immunity. In this study, we firstly cloned SOCS-1 (goSOCS-1) from duck Tembusu virus (DTMUV) infected goose. The full-length sequence of goSOCS-1 ORF is 624bp and encoded 108 amino acids. Read More

    Visualization of hepatitis E virus RNA and proteins in the human liver.
    J Hepatol 2017 Apr 12. Epub 2017 Apr 12.
    Department of Pathology and Molecular Pathology, University Zurich and University Hospital Zurich, Zurich, Switzerland. Electronic address:
    Background: / Aims: Although hepatitis E constitutes a substantial disease burden worldwide, surprisingly little is known about the localization of hepatitis E virus (HEV) in the human liver. We therefore aimed to visualize HEV RNA and proteins in situ.

    Methods: A panel of 12 different antibodies against HEV open reading frame (ORF) 1-3 proteins was evaluated for immunohistochemistry (IHC) and two probes for in situ hybridization (ISH) in formalin-fixed, paraffin-embedded (FFPE) Huh-7 cells transfected with HEV ORF1-3 expression vectors. Read More

    Utilization of defined microbial communities enables effective evaluation of meta-genomic assemblies.
    BMC Genomics 2017 Apr 13;18(1):296. Epub 2017 Apr 13.
    Human Longevity Inc, San Diego, CA, USA.
    Background: Metagenomics is the study of the microbial genomes isolated from communities found on our bodies or in our environment. By correctly determining the relation between human health and the human associated microbial communities, novel mechanisms of health and disease can be found, thus enabling the development of novel diagnostics and therapeutics. Due to the diversity of the microbial communities, strategies developed for aligning human genomes cannot be utilized, and genomes of the microbial species in the community must be assembled de novo. Read More

    Review of poxvirus: emergence of monkeypox.
    Med Sante Trop 2017 Feb;27(1):29-39
    Service de dermatologie, HIA Sainte-Anne, Toulon, France.
    This article reviews the different types of poxvirus infections. Smallpox, although eradicated, must continue to be monitored because of the potential risk of accidental or voluntary (by bioterrorism) reintroduction. Monkeypox and cowpox viruses are considered to be emergent today ; their high risk of dissemination is due to the increase in international transport as well as trends for new animals as pets and the loss of vaccinal protection against smallpox. Read More

    Rv3634c from Mycobacterium tuberculosis H37Rv encodes an enzyme with UDP-Gal/Glc and UDP-GalNAc 4-epimerase activities.
    PLoS One 2017 12;12(4):e0175193. Epub 2017 Apr 12.
    Department of Biosciences and Bioengineering Indian Institute of Technology Bombay Powai, Mumbai, India.
    A bioinformatics study revealed that Mycobacterium tuberculosis H37Rv (Mtb) contains sequence homologs of Campylobacter jejuni protein glycosylation enzymes. The ORF Rv3634c from Mtb was identified as a sequence homolog of C. jejuni UDP-Gal/GalNAc 4-epimerase. Read More

    PhiC31 integrase can improve the efficiency of different construct designs for monoclonal antibody expression in CHO cells.
    Protein Expr Purif 2017 Apr 9;134:89-95. Epub 2017 Apr 9.
    Biotechnology Research Center, Pasteur Institute of Iran, Tehran, Iran. Electronic address:
    Objectives: Several types of expression vectors have been used for recombinant protein expression in Chinese hamster ovary cells (CHO) which usually result in variable and unstable levels of expression.

    Methods And Results: In this study, we have compared the mAb0014 expression level of single ORF/IRES vector and dual ORF vector in the presence and absence of phiC31 integrase targeting system. Both expression vectors contain an elongation factor 1α (EF1α) promoter upstream of LC and harboring an attB site. Read More

    LTR-Retrotransposons from Bdelloid Rotifers Capture Additional ORFs Shared between Highly Diverse Retroelement Types.
    Viruses 2017 Apr 11;9(4). Epub 2017 Apr 11.
    Josephine Bay Paul Center for Comparative Molecular Biology and Evolution, Marine Biological Laboratory, 7 MBL Street, Woods Hole, MA 02543, USA.
    Rotifers of the class Bdelloidea, microscopic freshwater invertebrates, possess a highlydiversified repertoire of transposon families, which, however, occupy less than 4% of genomic DNA in the sequenced representative Adineta vaga. We performed a comprehensive analysis of A. vaga retroelements, and found that bdelloid long terminal repeat (LTR)retrotransposons, in addition to conserved open reading frame (ORF) 1 and ORF2 corresponding to gag and pol genes, code for an unusually high variety of ORF3 sequences. Read More

    Genotype analysis of noroviruses associated with gastroenteritis outbreaks in childcare centres, Victoria, Australia, 2012-2015.
    Epidemiol Infect 2017 Apr 11:1-9. Epub 2017 Apr 11.
    Victorian Infectious Diseases Reference Laboratory,Doherty Institute,Melbourne, Victoria,Australia.
    The characteristics of norovirus outbreaks in children (0-5 years) in childcare centres in Victoria, Australia (2012-2015) were examined. The three most common open reading frame (ORF) 2 genotypes in childcare centre outbreaks were GII.4 (42%), GII. Read More

    Natural non-homologous recombination led to the emergence of a duplicated V3-NS5A region in HCV-1b strains associated with hepatocellular carcinoma.
    PLoS One 2017 10;12(4):e0174651. Epub 2017 Apr 10.
    IRHS, Université d'Angers, SFR4207 QUASAV, Angers, France.
    Background: The emergence of new strains in RNA viruses is mainly due to mutations or intra and inter-genotype homologous recombination. Non-homologous recombinations may be deleterious and are rarely detected. In previous studies, we identified HCV-1b strains bearing two tandemly repeated V3 regions in the NS5A gene without ORF disruption. Read More

    Phylogenetic position of the coral symbiont Ostreobium (Ulvophyceae) inferred from chloroplast genome data.
    J Phycol 2017 Apr 10. Epub 2017 Apr 10.
    School of BioSciences, University of Melbourne, Melbourne, Victoria, 3010, Australia.
    The green algal genus Ostreobium is an important symbiont of corals, playing roles in reef decalcification and providing photosynthates to the coral during bleaching events. A chloroplast genome of a cultured strain of Ostreobium was available, but low taxon sampling and Ostreobium's early-branching nature left doubt about its phylogenetic position. Here we generate and describe chloroplast genomes from four Ostreobium strains as well as Avrainvillea mazei and Neomeris sp. Read More

    Single-Round Infectious Particle Antiviral Screening Assays for the Japanese Encephalitis Virus.
    Viruses 2017 Apr 10;9(4). Epub 2017 Apr 10.
    Department of Medical Laboratory Science and Biotechnology, China Medical University, Taichung 40402, Taiwan.
    Japanese Encephalitis virus (JEV) is a mosquito-borne flavivirus with a positive-sense single-stranded RNA genome that contains a big open reading frame (ORF) flanked by 5'- and 3'- untranslated regions (UTRs). Nearly 30,000 JE cases with 10,000 deaths are still annually reported in East Asia. Although the JEV genotype III vaccine has been licensed, it elicits a lower protection against other genotypes. Read More

    A comparison of the adaptations of strains of Lymantria dispar multiple nucleopolyhedrovirus to hosts from spatially isolated populations.
    J Invertebr Pathol 2017 Apr 7;146:41-46. Epub 2017 Apr 7.
    Laboratory of Insect Pathology, Institute of Systematics and Ecology of Animal SB RAS, Frunze str. 11, 630091 Novosibirsk, Russia. Electronic address:
    The adaptation of pathogens to either their hosts or to environmental conditions is the focus of many current ecological studies. In this work we compared the ability of six spatially-distant Lymantria dispar (gypsy moth) multiple nucleopolyhedrovirus (LdMNPV) strains (three from eastern North America and three from central Asia) to induce acute infection in gypsy moth larvae. We also sequenced the complete genome of one Asian (LdMNPV-27/0) and one North American (LdMNPV-45/0) strain which were used for bioassay. Read More

    Defect in the GTPase activating protein (GAP) function of eIF5 causes repression of GCN4 translation.
    Biochem Biophys Res Commun 2017 May 4;486(4):1110-1115. Epub 2017 Apr 4.
    School of Biological Sciences, National Institute of Science Education and Research Bhubaneswar, Constituent Institutes of Homi Bhabha National Institute (HBNI), P.O Jatni, Khurda 752050 India. Electronic address:
    In eukaryotes, the eIF5 protein plays an important role in translation start site selection by providing the GAP (GTPase activating protein) function. However, in yeast translation initiation fidelity defective eIF5(G31R) mutant causes preferential utilization of UUG as initiation codon and is termed as Suppressor of initiation codon (Sui(-)) phenotype due to its hyper GTPase activity. The eIF5(G31R) mutant dominantly represses GCN4 expression and confers sensitivity to 3-Amino-1,2,4-Trizole (3AT) induced starvation. Read More

    The molecular epidemiology of norovirus outbreaks in Victoria, 2014 to 2015
.
    Commun Dis Intell Q Rep 2017 Mar 31;41(1):E21-E32. Epub 2017 Mar 31.
    Head, Viral Gastroenteritis Section Victorian Infectious Diseases Reference Laboratory, Melbourne, Victoria.
    Noroviruses are a leading cause of outbreaks of gastroenteritis. This study examined the incidence and molecular characteristics of norovirus outbreaks in healthcare and non-healthcare settings in Victoria, Australia, over 2 years (2014-2015). Norovirus was detected in 65. Read More

    Genetic characterization of orf virus associated with an outbreak of severe orf in goats at a farm in Lusaka, Zambia (2015).
    Arch Virol 2017 Apr 4. Epub 2017 Apr 4.
    Department of Disease Control, School of Veterinary Medicine, University of Zambia, PO Box 32379, Lusaka, Zambia.
    Orf or contagious ecthyma is a neglected and economically important zoonotic disease caused by a dermatotropic parapoxvirus that commonly affects domestic small ruminants. Although orf is globally distributed, there is a paucity of information on the disease in many African countries. Here, a suspected severe outbreak of orf in goats at a farm in Lusaka was investigated. Read More

    A novel enterovirus species identified from severe diarrheal goats.
    PLoS One 2017 4;12(4):e0174600. Epub 2017 Apr 4.
    College of Veterinary Medicine, Jilin University, Changchun, Jilin, China.
    Backgrounds: The Enterovirus genus of the family of Picornaviridae consists of 9 species of Enteroviruses and 3 species of Rhinoviruses based on the latest virus taxonomy. Those viruses contribute significantly to respiratory and digestive disorders in human and animals. Out of 9 Enterovirus species, Enterovirus E-G are closely related to diseases affecting on livestock industry. Read More

    Development and validation of a highly sensitive real-time PCR assay for rapid detection of parapoxviruses.
    J Vet Diagn Invest 2016 Dec 1:1040638716680676. Epub 2016 Dec 1.
    Reagents and Vaccines Services Section (Das, Ward, Lowe, Xu, Jia).
    Parapoxviruses (PaPVs) cause widespread infections in ruminants worldwide. All PaPVs are zoonotic and may infect humans after direct or indirect contact with infected animals. Herein we report the development and validation of a highly sensitive real-time PCR assay for rapid detection of PaPVs. Read More

    The complete genomic sequence of a novel alphapartitivirus from Bipolaris maydis, the causal agent of corn southern leaf blight.
    Arch Virol 2017 Apr 3. Epub 2017 Apr 3.
    State Key Laboratory Breeding Base of Green Pesticide and Agricultural Bioengineering, Key Laboratory of Green Pesticide and Agricultural Bioengineering, Ministry of Education, Guizhou University, Guiyang, 550025, Guizhou, China.
    A novel double-stranded RNA (dsRNA) mycovirus, designated Bipolaris maydis partitivirus 1 (BmPV1), was isolated from the plant pathogenic fungus Bipolaris maydis. The BmPV1 genome has two dsRNA segments. The larger segment (1,930 bp) has a single open reading frame (ORF) with a conserved RNA-dependent RNA polymerase domain. Read More

    Molecular cloning, mRNA expression and nutritional regulation of a Δ6 fatty acyl desaturase-like gene of mud crab, Scylla paramamosain.
    Comp Biochem Physiol B Biochem Mol Biol 2017 Mar 31;208-209:29-37. Epub 2017 Mar 31.
    Guangdong Provincial Key Laboratory of Marine Biology, Shantou University, Shantou 515063, PR China. Electronic address:
    Fatty acyl desaturases (Fads) are critical enzymes in the pathways for the biosynthesis of the highly unsaturated fatty acids (HUFA). Here we report on the molecular cloning, tissue expression and nutritional regulation of a Δ6 fatty acyl desaturase-like (Δ6 Fad-like) gene from mud crab, Scylla paramamosain. The full-length cDNA was 1973bp, with a 201bp of 5'-UTR, a 443bp of 3'-UTR, and an ORF of 1329bp that encoded a protein of 442 amino acids. Read More

    Novel effects of identified SNPs within the porcine Pregnancy-Associated Glycoprotein gene family (pPAGs) on the major reproductive traits in Hirschmann hybrid-line sows.
    Res Vet Sci 2017 Mar 29;114:123-130. Epub 2017 Mar 29.
    Department of Animal Physiology, Faculty of Biology and Biotechnology, University of Warmia and Mazury in Olsztyn, ul. Oczapowskiego 1A, 10-719 Olsztyn-Kortowo, Poland.
    This is the first study describing identification of SNPs within the multiple and polymorphic Pregnancy-Associated Glycoprotein gene family (PAGs) in the genome of the domestic pig (pPAGs). We identified pPAG-like (pPAG-L) genotypes in primiparous and multiparous farmed hybrid-line JSR Hirschmann (Hrn) sows (N=159), in which various novel associations with their phenotypes for the major reproductive traits have been discovered. Genomic DNA templates were isolated from the blood and different pPAG-L primers were used to amplify various regions by PCR. Read More

    AcuC, a histone deacetylase, contributes to the pathogenicity of Aeromonas hydrophila.
    Microbiologyopen 2017 Mar 30. Epub 2017 Mar 30.
    Fisheries College, Key Laboratory of Healthy Mariculture for the East China Sea, Ministry of Agriculture, Jimei University, Xiamen, China.
    The interactions of pathogens and phagocytes are complex. Our study demonstrated that Aeromonas hydrophila B11 can survive in the macrophagocytes of Tilapia mossambica. To explore the regulatory processes of A. Read More

    Molecular analysis of norovirus in specimens from children enrolled in a 1982-1986 study in Belém, Brazil: A community-based longitudinal study.
    J Med Virol 2017 Mar 29. Epub 2017 Mar 29.
    Virology Section, Evandro Chagas Institute, Health Surveillance Secretariat, Brazilian Ministry of Health, Ananindeua, Pará, 67030-000, Brazil.
    Fecal specimens were collected during a longitudinal, community-based study in the city of Belém, North Brazil, that was conducted over 3 years (October 1982 to March 1986), in which 20 children were included from birth to 3 years of age. A total of 229 fecal samples were screened by real time RT-PCR targeting the junction region (ORF 1/2) of the norovirus (NoV) genome. NoV-positive samples were subjected to PCR and sequencing of the viral polymerase (ORF1) and viral protein 1 (VP1) genes (ORF2). Read More

    Sucrose sensing through nascent peptide-meditated ribosome stalling at the stop codon of Arabidopsis bZIP11 uORF2.
    FEBS Lett 2017 Mar 30. Epub 2017 Mar 30.
    Gene Center, Department of Biochemistry and Center for integrated Protein Science Munich (CiPSM), Ludwig-Maximilians-Universität München, Germany.
    Arabidopsis bZIP11 is a transcription factor that modulates amino acid metabolism under high-sucrose conditions. Expression of bZIP11 is downregulated in a sucrose-dependent manner during translation. Previous in vivo studies have identified the second upstream open reading frame (uORF2) as an essential regulatory element for the sucrose-dependent translational repression of bZIP11. Read More

    Non-coding RNA may be associated with cytoplasmic male sterility in Silene vulgaris.
    J Exp Bot 2017 Mar 29. Epub 2017 Mar 29.
    Institute of Experimental Botany v.v.i, Academy of Sciences of the Czech Republic, Rozvojová 263, Prague, 16502Czech Republic.
    Cytoplasmic male sterility (CMS) is a widespread phenomenon in flowering plants caused by mitochondrial (mt) genes. CMS genes typically encode novel proteins that interfere with mt functions and can be silenced by nuclear fertility-restorer genes. Although the molecular basis of CMS is well established in a number of crop systems, our understanding of it in natural populations is far more limited. Read More

    Cowpox virus infection in a child after contact with a domestic cat: a case report.
    New Microbiol 2017 Apr 3;40(2). Epub 2017 Apr 3.
    Department of Medical Microbiology, Poznań University of Medical Sciences, Poland.
    Human cowpox represents a seldom diagnosed zoonosis but this diagnosis should be considered more frequently as the number of cases has increased in recent years. We describe a case of cowpox in an 11-year-old boy following regular direct daily contact with a domestic cat. The 11-year-old patient, an otherwise healthy boy, demonstrated skin ulceration located at his chin, with enlargement of regional lymph nodes and fever reaching 39°C. Read More

    Molecular characterization and antibacterial activity of a phage-type lysozyme from the Manila clam, Ruditapes philippinarum.
    Fish Shellfish Immunol 2017 Mar 30;65:17-24. Epub 2017 Mar 30.
    Key Laboratory of Coastal Zone Environmental Processes, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai 264003, PR China; Muping Coastal Environment Research Station, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai 264003, PR China. Electronic address:
    A phage-type lysozyme, designed as RpPLYZ, was cloned and characterized from the clam Ruditapes philippinarum. The full-length cDNA of RpPLYZ was of 699 bp with an open reading frame (ORF) of 534 bp, encoding a polypeptide of 177-amino acid with a calculated molecular mass of 19.6 kDa and an isoelectric point of 9. Read More

    Glycogen synthase kinase-3 (GSK3) regulates TNF production and haemocyte phagocytosis in the immune response of Chinese mitten crab Eriocheir sinensis.
    Dev Comp Immunol 2017 Mar 29;73:144-155. Epub 2017 Mar 29.
    Functional Laboratory of Marine Fisheries Science and Food Production Process, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266200, China; Liaoning Key Laboratory of Marine Animal Immunology and Disease Control, Dalian Ocean University, Dalian 116023, China.
    Glycogen synthase kinase-3 (GSK3) is a serine/threonine protein kinase firstly identified as a regulator of glycogen synthesis. Recently, it has been proved to be a key regulator of the immune reaction. In the present study, a GSK3 homolog gene (designated as EsGSK3) was cloned from Chinese mitten crab, Eriocheir sinensis. Read More

    Purifying, cloning and characterizing a novel dehalogenase from Bacillus sp. GZT to enhance the biodegradation of 2,4,6-tribromophenol in water.
    Environ Pollut 2017 Jun 27;225:104-111. Epub 2017 Mar 27.
    State Key Laboratory of Organic Geochemistry and Guangdong Key Laboratory of Environmental Protection and Resources Utilization, Guangzhou Institute of Geochemistry, Chinese Academy of Sciences, Guangzhou 510640, China; Guangzhou Key Laboratory of Environmental Catalysis and Pollution Control, School of Environmental Science and Engineering, Institute of Environmental Health and Pollution Control, Guangdong University of Technology, Guangzhou, 510006, China. Electronic address:
    2,4,6-Tribromophenol (TBP), an intermediate of brominated flame retardants, can easily release to environment and recalcitrant to degradation. Previously, Bacillus sp. GZT, a pure aerobic strain capable of simultaneously debrominating and mineralizing TBP, was successfully isolated by us. Read More

    First report in Africa of two clinical isolates of Proteus mirabilis carrying Salmonella genomic island (SGI1) variants, SGI1-PmABB and SGI1-W.
    Infect Genet Evol 2017 Mar 28;51:132-137. Epub 2017 Mar 28.
    Laboratory of Food Microbiology and Hygiene, Graduate School of Biosphere Science, Hiroshima University, Higashi-Hiroshima 739-8528, Japan. Electronic address:
    Two Proteus mirabilis strains, designated PmTAN59 and PmKAF126, were isolated from two different Egyptian cities in 2014 and 2015, respectively. PmTAN59 was isolated from a sputum swab from a pneumonia patient in Tanta University Teaching Hospital. PmKAF126 was isolated from a patient with a diabetic foot infection in a hospital in the city of Kafr El-Sheikh. Read More

    Processing and targeting of proteins derived from polyprotein with 2A and LP4/2A as peptide linkers in a maize expression system.
    PLoS One 2017 30;12(3):e0174804. Epub 2017 Mar 30.
    Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing, China.
    In the transformation of multiple genes, gene fusion is an attractive alternative to other methods, including sexual crossing, re-transformation, and co-transformation, among others. The 2A peptide from the foot-and-mouth disease virus (FMDV) causes the co-translational "cleavage" of polyprotein and operates in a wide variety of eukaryotic cells. LP4, a linker peptide that originates from a natural polyprotein occurring in the seed of Impatiens balsamina, can be split between the first and second amino acids in post-translational processing. Read More

    Molecular Cloning, Identification, and Expression Patterns of Myostatin Gene in Water Buffalo (Bubalus Bubalis).
    Anim Biotechnol 2017 Mar 29:1-8. Epub 2017 Mar 29.
    a State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources , Guangxi University , Nanning , Guangxi , China.
    Myostatin (MSTN), also named growth differentiation factor 8 (GDF8), is a transforming growth factor-β (TGF-β) family member with a key role in the negative regulation of skeletal muscle growth. However, its role in ovarian folliculogenesis remains unclear. To provide us with a basis for understanding this role, we cloned MSTN and examined its expression patterns in water buffalo (Bubalus bubalis). Read More

    Isolation and Characterization of Encephalomyocarditis Virus from Dogs in China.
    Sci Rep 2017 Mar 27;7(1):438. Epub 2017 Mar 27.
    Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, 100193, China.
    Encephalomyocarditis virus (EMCV) is as a potential zoonotic agent with a wide host range. Here, we describe an EMC virus isolate, identified as EMCV C15, which was successfully obtained from the serum of dogs from animal hospitals. Virus production in cell culture was confirmed by EMCV-specific real-time RT-PCR, indirect immunofluorescence assays and electron microscopy. Read More

    Codon Optimization Leads to Functional Impairment of RD114-TR Envelope Glycoprotein.
    Mol Ther Methods Clin Dev 2017 Mar 11;4:102-114. Epub 2017 Jan 11.
    MolMed S.p.A., 20132 Milano, Italy.
    Lentiviral vectors (LVs) are a highly valuable tool for gene transfer currently exploited in basic, applied, and clinical studies. Their optimization is therefore very important for the field of vectorology and gene therapy. A key molecule for LV function is the envelope because it guides cell entry. Read More

    Mitochondrial genome of the nematode endoparasitic fungus Hirsutella vermicola reveals a high level of synteny in the family Ophiocordycipitaceae.
    Appl Microbiol Biotechnol 2017 Apr 24;101(8):3295-3304. Epub 2017 Mar 24.
    Institute of Applied Chemistry, Shanxi University, Taiyuan, 030006, China.
    Ophiocordycipitaceae is a diverse fungal family comprising multiple ecologically, economically, medicinally, and culturally important fungal species; however, only four species of the family have available mitochondrial genomes (mitogenomes). In this study, the complete mitogenome of the nematode endoparasitic fungus Hirsutella vermicola in Ophiocordycipitaceae was sequenced, and a comparative mitogenomic analysis of Ophiocordycipitaceae was performed. We found that the 53,793-bp circular mitogenome of H. Read More

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