591,297 results match your criteria Nucleic acids research[Journal]


Primary cultured neuronal networks and type 2 diabetes model mouse fatty liver tissues in aqueous liquid observed by atmospheric SEM (ASEM): Staining preferences of metal solutions.

Micron 2018 Nov 23;118:9-21. Epub 2018 Nov 23.

Biomedical Research Institute, National Institute of Industrial Science and Technology (AIST), Higashi 1-1-1, Tsukuba, Ibaraki, 305-8566, Japan. Electronic address:

Neural networking, including axon targeting and synapse formation, is the basis of various brain functions, including memory and learning. Diabetes-mellitus affects peripheral nerves and is known to cause fatty liver disease. Electron microscopy (EM) provides the resolution required to observe changes in fine subcellular structures caused by such physiological and pathological processes, but samples are observed in vacuum. Read More

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November 2018

Successful genetic modification of porcine spermatogonial stem cells via an electrically responsive Au nanowire injector.

Biomaterials 2018 Dec 10;193:22-29. Epub 2018 Dec 10.

Department of Animal Life Science, Kangwon National University, Chuncheon, 24341, South Korea; Department of Applied Animal Science, Kangwon National University, Chuncheon, 24341, South Korea. Electronic address:

Transgenic pigs are quite useful in many biomedical fields, such as xenotransplantation research and the production of biopharmaceutical materials. The genetic transformation of porcine spermatogonial stem cells (pSSCs) followed by differentiation into mature spermatozoa enables the effective production of transgenic pigs. Improving the transfection efficiency of pSSCs, however, has been much desired. Read More

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December 2018

Bubble-free rapid microfluidic PCR.

Biosens Bioelectron 2018 Oct 11;126:725-733. Epub 2018 Oct 11.

Department of Bioengineering, University of California Berkeley, CA 94720, USA; Berkeley Sensor and Actuator Center, University of California Berkeley, CA 94720, USA; UC Berkeley and UCSF Joint Graduate Program in Bioengineering, Berkeley/San Francisco, USA; Department of Electrical Engineering and Computer Science, University of California Berkeley, CA 94720, USA; Biophysics Graduate Program, University of California Berkeley, CA 94720, USA. Electronic address:

Microfluidic polymerase chain reaction (PCR) has been of great interest owing to its ability to perform rapid and specific nucleic acid amplification and analysis on small volumes of samples. One of the major drawbacks of microfluidic PCR is bubble generation and reagent evaporation, which can cause malfunctions. Here, through theoretical modeling and characterization of bubble behavior, we propose a bubble-free microfluidic PCR device via controlled fluid transfer. Read More

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October 2018
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A decade of Nucleic Acid Programmable Protein Arrays (NAPPA) availability: News, actors, progress, prospects and access.

J Proteomics 2018 Dec 12. Epub 2018 Dec 12.

Proteomics Unit, Cancer Research Centre (IBMCC/CSIC/USAL/IBSAL), 37007 Salamanca, Spain; Department of Medicine and General Cytometry Service-Nucleus, Cancer Research Centre (IBMCC/CSIC/USAL/IBSAL), 37007 Salamanca, Spain. Electronic address:

Understanding the dynamic of the proteome is a critical challenge because it requires high sensitive methodologies in high-throughput formats in order to decipher its modifications and complexity. While molecular biology provides relevant information about cell physiology that may be reflected in post-translational changes, High-Throughput (HT) experimental proteomic techniques are essential to provide valuable functional information of the proteins, peptides and the interconnections between them. Hence, many methodological developments and innovations have been reported during the last decade. Read More

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December 2018

Zika Virus Diagnosis: Challenges and Solutions.

Clin Microbiol Infect 2018 Dec 12. Epub 2018 Dec 12.

Department of Medicine, University of Miami Miller School of Medicine. Miami, FL, 33133 USA.

Background: Since its sudden appearance and link to microcephaly in 2015, the number of PubMed references for Zika virus (ZIKV) has risen from 181 to 5,163, as of writing, with a vast proportion focused on the consequences of ZIKV infection during pregnancy. This level of attention underlies increased demand for sensitive and specific diagnostic tools able to assess risk to an unborn child, as well as to understand the dynamics and consequences of viral persistence.

Aim: Review the expanding knowledge on ZIKV persistence and diagnostic challenges and summarize current advancements in detection. Read More

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December 2018

Magnetic beads assay based on zip nucleic acid for electrochemical detection of factor V Leiden mutation.

Int J Biol Macromol 2018 Dec 12. Epub 2018 Dec 12.

Faculty of Pharmacy, Analytical Chemistry Department, Ege University, Bornova, Izmir 35100, Turkey; Biotechnology Department, Graduate School of Natural and Applied Sciences, Ege University, Bornova, Izmir 35100, Turkey.

Single nucleotide polymorphisms (SNPs) are the most common type of genetic variation among people. Development of reliable methods for the detection of SNP is crucial in aspects of molecular diagnosis and personalized medicine. In our study, a genomagnetic assay in combination with zip nucleic acid (ZNA) for electrochemical detection of SNP related to Factor V Leiden (FV Leiden) mutation. Read More

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December 2018

Stimulation of TRPM3 channels increases the transcriptional activation potential of Elk-1 involving cytosolic Ca, extracellular signal-regulated protein kinase, and calcineurin.

Eur J Pharmacol 2018 Dec 12. Epub 2018 Dec 12.

Department of Medical Biochemistry and Molecular Biology Saarland University Medical Faculty D-66421 Homburg, Germany. Electronic address:

Stimulation of transient receptor potential M3 (TRPM3) channels with the steroid pregnenolone sulfate increases the transcriptional activation potential of Elk-1, a transcription factor that regulates serum response element-mediated transcription. Here, we show that an influx of Ca ions into the cells is essential for the activation of Elk-1 following stimulation of TRPM3. Using genetically encoded Ca buffers, we show that a rise in cytoplasmic Ca is required for the upregulation of the transcriptional activation potential of Elk-1, while buffering of Ca in the nucleus had no inhibitory effect on the transcriptional activity of Elk-1. Read More

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December 2018

Nucleus-targeted organoiridium-albumin conjugate for photoactivated cancer therapy.

Angew Chem Int Ed Engl 2018 Dec 15. Epub 2018 Dec 15.

University of Warwick, Chemistry, Gibbet Hill Road, CV4 7AL, Coventry, UNITED KINGDOM.

: A highly phosphorescent organoiridium-albumin conjugate (Ir1-HSA) was synthesized by reacting an organo-iridium complex (Ir1) containing a pendant maleimide with human serum albumin. The phosphorescence of the Ir1-HSA conjugate is much stronger than the parent iridium complex. Real-time cellular imaging shows that the conjugate mainly accumulates in the nucleus of cancer cells. Read More

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December 2018

Multiple intramolecular trafficking signals in RESISTANCE TO POWDERY MILDEW 8.2 are engaged in activation of cell death and defense.

Plant J 2018 Dec 15. Epub 2018 Dec 15.

Center for Crop Disease and Insect Pests, Rice Research Institute, Sichuan Agricultural University, Chengdu, 611130, China.

The extrahaustorial membrane (EHM) is a host-derived interfacial membrane encasing the haustorium of powdery mildew fungi. The Arabidopsis thaliana RESISTANCE TO POWDERY MILDEW 8.2 (RPW8. Read More

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December 2018

Thioamide-bridged nucleic acid (thioAmNA) bearing thymine or 2-thiothymine: Duplex forming ability, base discrimination, and enzymatic stability.

Chembiochem 2018 Dec 14. Epub 2018 Dec 14.

Osaka University, Graduate School of Pharmaceutical Sciences, 1-6 Yamadaoka, 565-0871, Suita, JAPAN.

Because oligonucleotides containing bridged nucleic acids (BNAs) show high duplex-forming ability toward target single-stranded RNA, many BNA analogs have been developed for antisense applications. Amide-bridged nucleic acid (AmNA), which is a BNA analog bearing an amide bond at the bridge, exhibits high duplex-forming ability, enzymatic stability, and antisense activity; thus, AmNA represents a promising BNA scaffold. The high enzymatic stability of AmNA is presumably attributable to the bulky amide structure because it inhibits the access of nucleases to the phosphodiester linkage. Read More

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December 2018

PAR2 promotes M1 macrophage polarization and inflammation via FOXO1 pathway.

J Cell Biochem 2018 Dec 14. Epub 2018 Dec 14.

Department of Physiology & Pathophysiology, School of Basic Medical Sciences, Shanghai Medical College, Fudan University, Shanghai, People's Republic of China.

Macrophages polarization plays essential but different roles in most diseases such as atherosclerosis, adipose tissue inflammation, and insulin resistance. Our previous study revealed that protease-activated receptor 2 (PAR2), a G-protein coupled receptor influenced macrophage function, but little is known regarding the regulation of macrophage polarization process and its potential mechanisms. In the present study, bone marrow-derived macrophages (BMDM) isolated from C57/BL6 mice and cultured with L929-conditional medium and murine macrophage cell line RAW264. Read More

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December 2018

Fastigial nucleus stimulation ameliorates cognitive impairment via modulating autophagy and inflammasomes activation in a rat model of vascular dementia.

J Cell Biochem 2018 Dec 14. Epub 2018 Dec 14.

Department of Neurology, The First Affiliated Hospital to Jinzhou Medical University, Jinzhou, China.

Background: It has been reported that the fastigial nucleus (FSN) plays an important role in the development of vascular dementia (VD). Both autophagy and inflammation are functionally involved in the pathogenesis of VD. In this study, we aimed to evaluate the therapeutic effect of electrical cerebellar fastigial nucleus stimulation (FNS) in VD treatment, as well as the effect of FNS on autophagy and inflammation. Read More

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December 2018

Identification of the gene encoding the TATA box-binding protein-associated factor 1 (TAF1) and its putative role in the heat shock response in the protozoan parasite Entamoeba histolytica.

Parasitol Res 2018 Dec 15. Epub 2018 Dec 15.

Departamento de Biología Celular, Cinvestav-IPN, Av. IPN 2508, Col. San Pedro Zacatenco, 07360, Ciudad de México, Mexico.

Transcription factor IID (TFIID) is a cornerstone in the transcription initiation in eukaryotes. It is composed of TBP and approximately 14 different subunits named TBP-associated factors (TAFs). TFIID has a key role in transcription of many genes involved in cell proliferation, cell growth, cell cycle, cell cycle checkpoint, and various other processes as well. Read More

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December 2018
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Nucleus-specific X-ray stain for 3D virtual histology.

Sci Rep 2018 Dec 14;8(1):17855. Epub 2018 Dec 14.

Department of Physics and Munich School of BioEngineering, Technical University of Munich, 85748, Garching, Germany.

Histological investigations are indispensable with regards to the identification of structural tissue details but are limited to two-dimensional images, which are often visualized in one and the same plane for comparison reasons. Nondestructive three-dimensional technologies such as X-ray micro- and nanoCT have proven to provide valuable benefits for the understanding of anatomical structures as they allow visualization of structural details in 3D and from arbitrary viewing angles. Nevertheless, low attenuation of soft tissue has hampered their application in the field of 3D virtual histology. Read More

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December 2018

Visualization of long-lived proteins reveals age mosaicism within nuclei of postmitotic cells.

J Cell Biol 2018 Dec 14. Epub 2018 Dec 14.

Molecular and Cell Biology Laboratory, The Salk Institute for Biological Studies, La Jolla, CA

Many adult tissues contain postmitotic cells as old as the host organism. The only organelle that does not turn over in these cells is the nucleus, and its maintenance represents a formidable challenge, as it harbors regulatory proteins that persist throughout adulthood. Here we developed strategies to visualize two classes of such long-lived proteins, histones and nucleoporins, to understand the function of protein longevity in nuclear maintenance. Read More

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December 2018

Synthesis of 2'-O-(N-methylcarbamoylethyl) 5-methyl-2-thiouridine and its application to splice-switching oligonucleotides.

Bioorg Med Chem Lett 2018 Dec 4. Epub 2018 Dec 4.

Department of Life Science and Technology, Tokyo Institute of Technology, 4259 J2-16, Nagatsuta-cho, Midori-ku, Yokohama, Kanagawa 226-8501, Japan. Electronic address:

The effect of 2'-O-(N-methylcarbamoyl)ethyl (MCE) modification on splice-switching oligonucleotides (SSO) was systematically evaluated. The incorporation of five MCE nucleotides at the 5'-termini of SSOs effectively improved the splice switching effect. In addition, the incorporation of 2'-O-(N-methylcarbamoylethyl)-5-methyl-2-thiouridine (sT), a duplex-stabilizing nucleotide with an MCE modification, into SSOs further improved splice switching. Read More

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December 2018

Can immune biomarkers predict infections in solid organ transplant recipients? A review of current evidence.

Transplant Rev (Orlando) 2018 Oct 9. Epub 2018 Oct 9.

Centre for Inflammatory Diseases, School of Clinical Sciences, Monash University, Australia; Department of Nephrology, Monash Medical Centre, Clayton, Victoria 3168, Australia. Electronic address:

Despite improvements in graft survival, solid organ transplantation is still associated with considerable infection induced morbidity and mortality. If we were able to show that serious infection risk was associated with excessive suppression of immune capacity, we would be justified in "personalizing" the extent of immunosuppression by carefully monitored reduction to see if we can improve immune compromize without increasing the risk of rejection. Reliable biomarkers are needed to identify this patients at an increased risk of infection. Read More

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October 2018

Activation of and in Transgenic Mouse Embryonic Stem Cells Increases Differentiation into Neural Crest Derivatives.

Int J Mol Sci 2018 Dec 13;19(12). Epub 2018 Dec 13.

Instituto de Fisiología Celular-Neurociencias, Universidad Nacional Autónoma de México, Ciudad Universitaria, Ciudad de México 04510, México.

The neural crest (NC) comprises a multipotent cell population that produces peripheral neurons, cartilage, and smooth muscle cells, among other phenotypes. The participation of and when expressed in mouse embryonic stem cells (mESCs) undergoing NC differentiation is unexplored. In this work, we generated stable mESCs transfected with constructs encoding chimeric proteins in which the ligand binding domain of glucocorticoid receptor (GR), which is translocated to the nucleus by dexamethasone addition, is fused to either (HGR) or (MGR), as well as double-transgenic cells (HGR+MGR). Read More

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December 2018

Pathological alterations of chondroitin sulfate moiety in postmortem hippocampus of patients with schizophrenia.

Psychiatry Res 2018 Dec 30;270:940-946. Epub 2018 Oct 30.

Department of Molecular Neurobiology, Brain Research Institute, Niigata University, 1-757, Asahimachi-dori, Chuo-ku Niigata, Niigata 951-8585, Japan. Electronic address:

Perineuronal nets comprise chondroitin sulfate moieties and their core proteins, and their neuropathological alterations have been implicated in schizophrenia. To explore the molecular mechanism of the perineuronal net impairments in schizophrenia, we measured the immunoreactivity of chondroitin sulfate moieties, major components of perineuronal nets, in three brain regions (postmortem dorsolateral prefrontal cortex, caudate nucleus, and hippocampus) of schizophrenia patients and control subjects. Immunoblotting for chondroitin 4-sulfate and chondroitin 6-sulfate moieties revealed a significant increase in intensity of a 180 kD band of chondroitin 4-sulfate immunoreactivity in the hippocampus of patients, although we detected no significant alteration in their immunoreactivities with any other molecular sizes or in other brain regions. Read More

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December 2018
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Filter paper grafted with epoxide-based copolymer brushes for activation-free peptide nucleic acid conjugation and its application for colorimetric DNA detection.

Colloids Surf B Biointerfaces 2019 Jan 27;173:851-859. Epub 2018 Sep 27.

Department of Chemistry, Faculty of Science, Chulalongkorn University, Phayathai Road, Pathumwan, Bangkok, 10330, Thailand; Center of Excellence in Materials and Bio-interfaces, Chulalongkorn University, Phayathai Road, Pathumwan, Bangkok 10330, Thailand. Electronic address:

Epoxide-bearing filter paper was first prepared by surface-initiated reversible addition-fragmentation chain transfer (RAFT) copolymerization of glycidyl methacrylate (GMA) and poly(ethylene glycol)methacrylate (PEGMA). Without the need for activation step, the capture peptide nucleic acid (PNA) probes carrying a C-terminal lysine modification can be directly immobilized on the surface-grafted poly[glycidyl methacrylate-ran-poly(ethylene glycol)methacrylate] (P(GMA-ran-PEGMA)) through ring-opening of epoxide groups in the GMA repeating units by amino groups in the PNA's structure. The success of P(GMA-ran-PEGMA) grafting on the filter paper and subsequent PNA immobilization was confirmed by fluorescence microscopy, Fourier transform-infrared spectroscopy and X-ray photoelectron spectroscopy. Read More

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January 2019

Structure of a (3+1) hybrid G-quadruplex in the PARP1 promoter.

Nucleic Acids Res 2018 Dec 15. Epub 2018 Dec 15.

School of Physical and Mathematical Sciences, Nanyang Technological University, Singapore 637371, Singapore.

Poly (ADP-ribose) polymerase 1 (PARP1) has emerged as an attractive target for cancer therapy due to its key role in DNA repair processes. Inhibition of PARP1 in BRCA-mutated cancers has been observed to be clinically beneficial. Recent genome-mapping experiments have identified a non-canonical G-quadruplex-forming sequence containing bulges within the PARP1 promoter. Read More

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December 2018
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CRISPR-C: circularization of genes and chromosome by CRISPR in human cells.

Nucleic Acids Res 2018 Dec;46(22):e131

Department of Biomedicine, Aarhus University, Denmark.

Extrachromosomal circular DNA (eccDNA) and ring chromosomes are genetic alterations found in humans with genetic disorders. However, there is a lack of genetic engineering tools to recapitulate and study the biogenesis of eccDNAs. Here, we created a dual-fluorescence biosensor cassette, which upon the delivery of pairs of CRISPR/Cas9 guide RNAs, CRISPR-C, allows us to study the biogenesis of a specific fluorophore expressing eccDNA in human cells. Read More

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December 2018

Functionality and cross-regulation of the four SprG/SprF type I toxin-antitoxin systems in Staphylococcus aureus.

Nucleic Acids Res 2018 Dec 15. Epub 2018 Dec 15.

Université de Rennes 1, Inserm, BRM (Bacterial Regulatory RNAs and Medicine) UMR_S 1230, 35000 Rennes, France.

Toxin-antitoxin (TA) systems are ubiquitous among bacteria, frequently expressed in multiple copies, and important for functions such as antibiotic resistance and persistence. Type I TA systems are composed of a stable toxic peptide whose expression is repressed by an unstable RNA antitoxin. Here, we investigated the functionalities, regulation, and possible cross-talk between three core genome copies of the pathogenicity island-encoded 'sprG1/sprF1' type I TA system in the human pathogen Staphylococcus aureus. Read More

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December 2018

Significant inhibition of photosynthesis and respiration in leaves of Cucumis sativus L. by oxybenzone, an active ingredient in sunscreen.

Chemosphere 2018 Dec 3;219:456-462. Epub 2018 Dec 3.

State Key Laboratory of Crop Biology, China; College of Life Sciences, Shandong Agricultural University, China. Electronic address:

Oxybenzone (OBZ), an active ingredient in most sunscreens, was recently shown to be toxic to humans, corals and other animals. This study is the first to demonstrate that OBZ can significantly inhibit photosynthesis and respiration in the leaves of a higher plant, cucumber. An OBZ suspension content as low as 0. Read More

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December 2018
1 Read

Detection of Mg-dependent, coaxial stacking rearrangements in a bulged three-way DNA junction by single-molecule FRET.

Biophys Chem 2018 Dec 5;245:25-33. Epub 2018 Dec 5.

Department of Physics, Loyola University Chicago, Chicago, IL, USA. Electronic address:

Three-way helical junctions (3WJs) arise in genetic processing, and they have architectural and functional roles in structured nucleic acids. An internal bulge at the junction core allows the helical domains to become oriented into two possible, coaxially stacked conformers. Here, the helical stacking arrangements for a series of bulged, DNA 3WJs were examined using ensemble fluorescence resonance energy transfer (FRET) and single-molecule FRET (smFRET) approaches. Read More

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December 2018
9 Reads

Synthesis of a new disulfide Fmoc monomer for creating biologically susceptible linkages in peptide nucleic acid oligomers.

Bioorg Chem 2018 Dec 1;84:394-398. Epub 2018 Dec 1.

Department of Chemistry and Biochemistry, Georgia Southern University, Savannah, GA, USA. Electronic address:

Peptide nucleic acids (PNA) are one of many synthetic mimics of DNA and RNA that have found applications as biological probes, as nano-scaffold components, and in diagnostics. In an effort to use PNA as constructs for cellular delivery we investigated the possibility of installing a biologically susceptible disulfide bond in the backbone of a PNA oligomer. Here we report the synthesis of a new abasic Fmoc monomer containing a disulfide bond that can be incorporated into a PNA oligomer (DS-PNA) using standard solid phase peptide synthesis. Read More

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December 2018

Asymmetric Expression of LincGET Biases Cell Fate in Two-Cell Mouse Embryos.

Cell 2018 Dec;175(7):1887-1901.e18

State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, 100101 Beijing, China; University of Chinese Academy of Sciences, 100049 Beijing, China; Institute for Stem Cell and Regeneration, Chinese Academy of Sciences, 100101 Beijing, China. Electronic address:

In early mammalian embryos, it remains unclear how the first cell fate bias is initially triggered and amplified toward cell fate segregation. Here, we report that a long noncoding RNA, LincGET, is transiently and asymmetrically expressed in the nucleus of two- to four-cell mouse embryos. Overexpression of LincGET in one of the two-cell blastomeres biases its progeny predominantly toward the inner cell mass (ICM) fate. Read More

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December 2018
2 Reads

Identification and immune-related analysis of SNPs in Litopenaeus vannamei Toll3 receptor.

Immunol Lett 2018 Dec 11. Epub 2018 Dec 11.

Department of Biology and Guangdong Provincial Key Laboratory of Marine Biotechnology, Shantou University, Shantou, 515063, China. Electronic address:

Tolls and Toll-like receptors (TLRs), as innate immune-recognition receptors that recognize molecular patterns associated with microbial pathogens, play a critical role in antimicrobial immune responses. Here, we report on single nucleotide polymorphisms (SNPs) of Litopenaeus vannamei Toll3 (LvToll3). Multiple sequence alignment of the L. Read More

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December 2018

Novel mass spectrometry based detection and identification of variants of rabies virus nucleoprotein in infected brain tissues.

PLoS Negl Trop Dis 2018 Dec 14;12(12):e0006984. Epub 2018 Dec 14.

Poxvirus and Rabies Branch, Centers for Disease Control and Prevention, Atlanta, GA, United States of America.

Human rabies is an encephalitic disease transmitted by animals infected with lyssaviruses. The most common lyssavirus that causes human infection is rabies virus (RABV), the prototypic member of the genus. The incubation period of RABV in humans varies from few weeks to several months in some instances. Read More

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December 2018

Extracellular Vesicle-Mimetic Ghost Nanovesicles for Delivering Anti-Inflammatory Drugs to Mitigate Gram-Negative Bacterial Outer Membrane Vesicle-Induced Systemic Inflammatory Response Syndrome.

Adv Healthc Mater 2018 Dec 14:e1801082. Epub 2018 Dec 14.

Department of Life Sciences, Pohang University of Science and Technology (POSTECH), 77 Cheongam-ro, Nam-gu, Pohang, 37673, Republic of Korea.

Sepsis is one of the major causes of death in hospital patients and is represented by systemic inflammatory response syndrome (SIRS) associated with infection. Gram-negative bacteria including Escherichia coli can provoke sepsis by stimulating the immune systems. Outer membrane vesicles (OMVs), nanosized vesicular structures derived from Gram-negative bacteria, contain several pathogen-associated molecular patterns, and are demonstrated to mediate SIRS. Read More

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December 2018

A Flow Cytometry-Based Assay for High-Throughput Detection and Quantification of Neutrophil Extracellular Traps in Mixed Cell Populations.

Cytometry A 2018 Dec 14. Epub 2018 Dec 14.

Department of Microbiology and Immunology, Yong Loo Lin School of Medicine, National University of Singapore, Singapore.

Neutrophil extracellular traps (NETs) are web-like structures composed of decondensed chromatin and antimicrobial proteins that are released into the extracellular space during microbial infections. This active cell death program is known as NETosis. To date, florescence microscopy is the widely accepted method for visualization and quantification of NETs. Read More

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December 2018

Beyond the Message: Advantages of Snapshot Proteomics with Single-Cell Mass Cytometry in Solid Tumors.

FEBS J 2018 Dec 13. Epub 2018 Dec 13.

Department of Cell and Developmental Biology, Vanderbilt University, Nashville, TN, USA.

Single-cell technologies that can quantify features of individual cells within a tumor are critical for treatment strategies aiming to target cancer cells while sparing or activating beneficial cells. Given that key players in protein networks are often the primary targets of precision oncology strategies, it is imperative to transcend the nucleic acid message and read cellular actions in human solid tumors. Here, we review the advantages of single-cell mass cytometry. Read More

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December 2018

RanGAP-mediated nucleocytoplasmic transport of Prospero regulates neural stem cell lifespan in Drosophila larval central brain.

Aging Cell 2018 Dec 13:e12854. Epub 2018 Dec 13.

Division of Human Reproduction and Developmental Genetics, The Women's Hospital, School of Medicine, Zhejiang University, Hangzhou, China.

By the end of neurogenesis in Drosophila pupal brain neuroblasts (NBs), nuclear Prospero (Pros) triggers cell cycle exit and terminates NB lifespan. Here, we reveal that in larval brain NBs, an intrinsic mechanism facilitates import and export of Pros across the nuclear envelope via a Ran-mediated nucleocytoplasmic transport system. In rangap mutants, the export of Pros from the nucleus to cytoplasm is impaired and the nucleocytoplasmic transport of Pros becomes one-way traffic, causing an early accumulation of Pros in the nuclei of the larval central brain NBs. Read More

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December 2018
6.340 Impact Factor

Clinical value of enzyme immunoassay detecting rubella-specific immunoglobulin M immediately after the disease onset.

Microbiol Immunol 2018 Dec 14. Epub 2018 Dec 14.

National Hospital Organization, Nagoya Medical Center, 4-1-1 Sannomaru, Naka-ku, Nagoya, 460-0001, Japan.

A total of 300 nucleic acid test (NAT)-confirmed rubella patients, mostly adults, were examined to address clinical value of rubella specific IgM test using an enzyme immunoassay (EIA) kit. The IgM titer increased after rash onset, and the median IgM titer at 3 days post-onset was significantly higher than those at previous days (p < 0.0001). Read More

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December 2018

EgRBP42 from oil palm enhances adaptation to stress in Arabidopsis through regulation of nucleocytoplasmic transport of stress-responsive mRNAs.

Plant Cell Environ 2018 Dec 13. Epub 2018 Dec 13.

Department of Cell and Molecular Biology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 UPM, Serdang, Selangor, Malaysia.

Abiotic stress reduces plant growth and crop productivity. However, the mechanism underlying post-transcriptional regulations of stress response remains elusive. Herein, we report the post-transcriptional mechanism of nucleocytoplasmic RNA transport of stress-responsive transcripts mediated by EgRBP42, an hnRNP-like RNA-binding protein from oil palm, which could be necessary for rapid protein translation to confer abiotic stress tolerance in plants. Read More

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December 2018

Detection of Epstein-Barr virus (EBV)-encoded microRNAs in plasma of patients with nasopharyngeal carcinoma.

Head Neck 2018 Dec 13. Epub 2018 Dec 13.

Division of Head and Neck, Department of Surgery, The University of Hong Kong, Hong Kong SAR, China.

Background: Nasopharyngeal carcinoma (NPC) latently infected by Epstein-Barr virus (EBV) expresses 40 EBV BART microRNAs (miRNAs). Difference in diagnostic efficacy of these miRNAs on NPC detection was observed. Here, we performed a comprehensive evaluation on the efficacy of these miRNAs. Read More

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December 2018

Hepatitis E virus blood donor NAT screening: as much as possible or as much as needed?

Transfusion 2018 Dec 13. Epub 2018 Dec 13.

Institut für Laboratoriums- und Transfusionsmedizin, Herz- und Diabeteszentrum Nordrhein- Westfalen, Universitätsklinik der Ruhr-Universität Bochum, Bad Oeynhausen, Germany.

Background: The cost-benefit question of general screening of blood products for the hepatitis E virus (HEV) is currently being discussed. One central question is the need for individual nucleic acid amplification techniques (NAT) screening (ID-NAT) versus minipool NAT screening (MP-NAT) approaches to identify all relevant viremias in blood donors. Here, the findings of ID-NAT versus MP-NAT in pools of 96 samples were compared. Read More

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December 2018

A comparison of the EU regulatory approach to directed mutagenesis with that of other jurisdictions, consequences for international trade and potential steps forward.

New Phytol 2018 Dec 13. Epub 2018 Dec 13.

Biotechnology Directorate, Ministry of AgroIndustry, Buenos Aires, Argentina; National University of Quilmes, Bernal, Argentina.

A special regulatory regime applies to products of recombinant nucleic acid modifications. A ruling from the European Court of Justice has interpreted this regulatory regime in a way that it also applies to emerging mutagenesis techniques. Elsewhere regulatory progress is also ongoing. Read More

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December 2018

Role of BMP signaling for the formation of auditory brainstem nuclei and large auditory relay synapses.

Dev Neurobiol 2018 Dec 12. Epub 2018 Dec 12.

Laboratory of Synaptic Mechanisms, Brain Mind Institute, School of Life Science, École Polytechnique Fédérale de Lausanne (EPFL), 1015, Lausanne, Switzerland.

Large excitatory synapses are found at specific points in the neuronal circuits of the auditory brainstem, to enable fast information transfer and the preservation of acoustic timing information. The extracellular cues and signaling mechanisms that lead to the development of these specialized synaptic connections, exemplified by the calyx of Held in the medial nucleus of the trapezoid body (MNTB), are still largely unknown. Here, we investigate the role of BMP signaling for the early development of the ventral cochlear nucleus (VCN) and MNTB, and for the initial formation of the calyx of Held synaptic connection. Read More

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December 2018

JAK2 regulates mismatch repair protein-mediated epigenetic alterations in response to oxidative damage.

Environ Mol Mutagen 2018 Dec 11. Epub 2018 Dec 11.

Medical Sciences, Indiana University School of Medicine, Bloomington, Indiana, United States of America, 47405.

At sites of chronic inflammation epithelial cells undergo aberrant DNA methylation that contributes to tumorigenesis. Inflammation is associated with an increase in reactive oxygen species (ROS) that cause oxidative DNA damage, which has also been linked to epigenetic alterations. We previously demonstrated that in response to ROS, mismatch repair (MMR) proteins MSH2 and MSH6 recruit epigenetic silencing proteins DNA methyltransferase 1 (DNMT1) and Polycomb repressive complex 2 (PRC2) members to sites of DNA damage, resulting in transcriptional repression of tumor suppressor genes (TSGs). Read More

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December 2018

Utility of microdissected cytology smears for molecular analysis of thyroid malignancy.

Diagn Cytopathol 2018 Dec 8. Epub 2018 Dec 8.

Interpace Diagnostics Lab, New Haven, Connecticut.

Background: Molecular testing of thyroid fine-needle aspirates has demonstrated value in cases of indeterminate cytology (Bethesda categories III, IV, and V) enabling optimized individual patient management leading to better outcomes with health economic benefits. For most molecular testing modalities, including mutational panels and classifier analyses, part or all of a dedicated needle aspiration pass is required to obtain an adequate sample for testing. Our analysis, which is based on a combination approach (mutation detection and microRNA classifier status), has documented clinical validity and utility when performed on thyroid fine-needle aspirates placed directly into RNA preservative fluid. Read More

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December 2018

Antisense Oligonucleotide-conjugated Nanostructure Targeting LncRNA MALAT1 Inhibits Cancer Metastasis.

ACS Appl Mater Interfaces 2018 Dec 14. Epub 2018 Dec 14.

Metastasis-associated lung adenocarcinoma transcript 1 (MALAT1), a long noncoding RNA (lncRNA) located in the cell nucleus, is a critical regulator of tumor cell migration. Antisense oligonucleotides (ASOs), which can downregulate the expression level of specific RNAs, have been used in clinical for diseases treatment. Herein, we constructed MALAT1-specific ASO and nucleus-targeting TAT peptide co-functionalized Au nanoparticles, namely ASO-Au-TAT NPs, which stabilized the fragile ASOs, enhanced nuclear internalization, and exhibited good biocompatibility. Read More

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December 2018

Targeted Loop-Mediated Isothermal Amplification (LAMP) Assay for Consensus Detection of Mycobacteria Associated With Pulmonary Infections.

Front Med (Lausanne) 2018 28;5:332. Epub 2018 Nov 28.

Aix-Marseille Université, IRD 198, MEPHI, IHU Méditerranée Infection, Marseille, France.

Loop-mediated isothermal amplification (LAMP) is a nucleic acid method which has been used to identify mycobacteria including in clinical microbiology laboratory and point of care settings. Previously published LAMP protocols for detection of mycobacterial species used conventional specific primer and targeted the 16S rRNA, , and insertion sequence genes. We developed and evaluated a LAMP assay targeting a mycobacterial gene conserved sequence and incorporating degenerate primers. Read More

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November 2018

The role of CMV in glioblastoma and implications for immunotherapeutic strategies.

Oncoimmunology 2019 16;8(1):e1514921. Epub 2018 Oct 16.

Lillian S. Wells Department of Neurosurgery, UF Brain Tumor Immunotherapy Program, Preston A. Wells, Jr. Center for Brain Tumor Therapy, University of Florida, Gainesville, FL, USA.

Controversy surrounds the role of cytomegalovirus (CMV) in glioblastoma (GBM). However, several studies have shown that CMV nucleic acids and proteins are present within GBM tumor tissue. CMV has been implicated in GBM pathogenesis by affecting tumor stem cell factors, angiogenesis and immune pathways. Read More

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October 2018
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Shank-associated RH domain-interacting protein expression is upregulated in entodermal and mesodermal cancer or downregulated in ectodermal malignancy.

Oncol Lett 2018 Dec 27;16(6):7180-7188. Epub 2018 Sep 27.

Department of Dermatology, Cosmetology and Venereology, Shenzhen Hospital, Southern Medical University, Shenzhen, Guangdong 518101, P.R. China.

Shank-associated RH domain-interacting protein (SHARPIN) is a type of linear ubiquitin chain-associated protein, which serves an important role in cell proliferation, apoptosis, organ development, immune and inflammatory reaction, initiation and development of malignant tumors. To evaluate SHARPIN expression in multiple malignant tumors derived from different germ layers, 14 types of cancer and their corresponding normal tissues were examined. Immunohistochemistry was performed to semi-quantify SHARPIN expression in multiple malignant tumors, and immunofluorescence was performed to evaluate the subcellular localization of SHARPIN in various malignant tumors. Read More

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December 2018

Osteocalcin triggers Fas/FasL-mediated necroptosis in adipocytes via activation of p300.

Cell Death Dis 2018 Dec 13;9(12):1194. Epub 2018 Dec 13.

School of Dental Medicine, Fukuoka Dental College, Fukuoka, 814-0193, Japan.

The uncarboxylated form of osteocalcin (GluOC) regulates glucose and lipid metabolism in mice. We previously showed that low-dose (≤10 ng/ml) GluOC induces the expression of adiponectin and peroxisome proliferator-activated receptor γ (PPARγ) via a cAMP-PKA-ERK-CREB signaling pathway in 3T3-L1 adipocytes. We also noticed that high-dose (≥20 ng/ml) GluOC inhibits the expression of adiponectin and PPARγ in these cells. Read More

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December 2018
5.014 Impact Factor

Lysosomotropic drugs activate TFEB via lysosomal membrane fluidization and consequent inhibition of mTORC1 activity.

Cell Death Dis 2018 Dec 13;9(12):1191. Epub 2018 Dec 13.

The Fred Wyszkowski Cancer Research Laboratory, Technion-Israel Institute of Technology, Haifa, Israel.

Transcription factor EB (TFEB) is a master transcriptional regulator playing a key role in lysosomal biogenesis, autophagy and lysosomal exocytosis. TFEB activity is inhibited following its phosphorylation by mammalian target of rapamycin complex 1 (mTORC1) on the surface of the lysosome. Phosphorylated TFEB is bound by 14-3-3 proteins, resulting in its cytoplasmic retention in an inactive state. Read More

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December 2018

Label-free optical biosensor for target detection based on simulation-assisted catalyzed hairpin assembly.

Comput Biol Chem 2018 Dec 3. Epub 2018 Dec 3.

College of Life Sciences, Shaanxi Normal University, Xi'an, 710119, China; National Engineering Laboratory for Resource Developing of Endangered Chinese Crude Drugs in Northwest of China. Electronic address:

The development of efficient and convenient strategy without involving enzyme or complex nanomaterial for the micro molecules detection has profound meaning in the diagnosis of diseases. Herein, taking the advantages of the strong affinity of aptamer and catalyzed hairpin assembly, we develop a new non-label optical amplified strategy for thrombin detection in this work. To support both biological inquiry and technological innovation, thermodynamic models are introduced to predict the minimum energy secondary structure of interacting nucleic acid strands and calculate the partition function and equilibrium concentration for complexes in our system. Read More

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December 2018

High-Throughput Electrophysiology Screen Revealed Cardiotoxicity of Strychnine by Selectively Targeting hERG Channel.

Am J Chin Med 2018 Dec 14:1-16. Epub 2018 Dec 14.

1 Tianjin State Key Laboratory of Modern, Chinese Medicine, Tianjin University of Traditional, Chinese Medicine, Tianjin 300193, P. R. China.

Although the efficacy and the health care advantages of Chinese herbal medicine (CHM) have become increasingly recognized worldwide, the potential side effects and toxicity still restrict its broader application. This study established and applied an integrated platform anchored on automatic patch clamp system to screen and evaluate a collection of CHM extracts, compositions and monomeric compounds for in vitro cardiac toxicity. Of 1036 CHM samples screened, 2. Read More

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December 2018
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Overexpression of the Wild Soybean R2R3-MYB Transcription Factor Enhances Resistance to Salt Stress and in Transgenic .

Int J Mol Sci 2018 Dec 9;19(12). Epub 2018 Dec 9.

Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture, Oil Crops Research Institute, Chinese Academy of Agricultural Sciences, Wuhan 430062, China.

Plant R2R3-MYB transcription factors (TFs) have been suggested to play crucial roles in the response to diverse abiotic and biotic stress factors but there is little molecular evidence of this role in soybean plants. In this work, we identified and functionally characterized an R2R3-MYB TF, namely, , from the wild soybean ED059. Protein and promoter sequence analysis indicated that is a typical R2R3-MYB TF and contains multiple stress-related elements in the promoter region. Read More

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December 2018
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