26,131 results match your criteria Nippon Yakurigaku Zasshi Folia Pharmacologica Japonica[Journal]


Long-term efficacy and dose-finding trial of Japanese cedar pollen SLIT tablet.

J Allergy Clin Immunol Pract 2018 Dec 8. Epub 2018 Dec 8.

Department of Otolaryngology, Head and Neck Surgery, Graduate School of Medicine, Nippon Medical School, Tokyo, Japan.

Background: Japanese cedar (JC) pollinosis is a common allergic rhinitis in Japan. The JC pollen SLIT tablet was developed using the highest concentration of JC pollen extract.

Objective: This was a randomized, double-blind, placebo-controlled phase II/III trial to investigate the optimal dose of the JC pollen SLIT tablet and examine long-term efficacy and safety for 3 years with the selected dose, and 2-year follow-up. Read More

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December 2018

YHp as a highly stable, hyper-copy, hyper-expression plasmid constructed using a full 2 μm circle sequence in cir0 strains of Saccharomyces cerevisiae.

Yeast 2018 Dec 7. Epub 2018 Dec 7.

Department of Applied Chemistry, Graduate School of Sciences and Technology for Innovation, Yamaguchi University, Ube, 755-8611, Japan.

In the yeast Saccharomyces cerevisiae, the plasmid YEp, containing a partial sequence from a natural 2 μm plasmid, has been frequently used to induce high levels of gene expression. In this study, we used Japanese sake yeast natural cir0 strain as a host for constructing an entire 2 μm plasmid with an expression construct using the three-fragment gap-repair method without Escherichia coli manipulation. The 2 μm plasmid contains two long inverted repeats, which is problematic for PCR amplification. Read More

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December 2018

Volume change in infrapatellar fat pad is associated not with obesity but with cartilage degeneration.

J Orthop Res 2018 Dec 11. Epub 2018 Dec 11.

Department of General Medical Services, Graduate School of Medicine, Chiba University, 1-8-1 Inohana, Chuo-ku, Chiba 260-8670, Japan.

Infrapatellar fat pad has been implicated in knee osteoarthritis. We examined whether infrapatellar fat pad volume is associated with quantitative cartilage changes using magnetic resonance imaging T1ρ mapping. Fifty-eight knees of knee pain patients (19 men, 39 women, mean age 57. Read More

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December 2018

Prospective exosome-focused translational research for afatinib study of non-small cell lung cancer patients expressing EGFR (EXTRA study).

Thorac Cancer 2018 Dec 8. Epub 2018 Dec 8.

Division of Medical Oncology, Department of Internal Medicine, Teikyo University School of Medicine, Tokyo, Japan.

Patients with EGFR-mutated non-small cell lung cancer (NSCLC) exhibit resistance to EGFR-tyrosine kinase inhibitors (TKIs) within 9-14 months of therapy. Recently, EGFR-mutated NSCLC has demonstrated the potential for heterogeneity; therefore, the manner of clonal heterogeneity may impact the duration of progression-free and overall survival and other parameters affecting EGFR-TKI treatment efficacy. However no predictive biomarker of these favorable treatment efficacies has been identified to date. Read More

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December 2018

Bonobos' saliva remaining on the pith of terrestrial herbaceous vegetation can serve as non-invasive wild genetic resources.

Primates 2018 Dec 10. Epub 2018 Dec 10.

Primate Research Institute, Kyoto University, Inuyama, Aichi, 484-8506, Japan.

Evaluating the genetic diversity of natural populations of endangered species is important for conservation. Although the genetic analysis of wildlife usually requires collecting DNA non-invasively, the variety of non-invasive DNA sampling methods is limited for each species. We present a method to obtain DNA of an endangered species, the bonobo (Pan paniscus), in which the pith of the terrestrial herbaceous vegetation (THV) that they consumed was newly utilized. Read More

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December 2018
1 Read

Posterior tibial slope angle is associated with flexion-type Salter-Harris II and Watson-Jones type IV fractures of the proximal tibia.

Knee Surg Sports Traumatol Arthrosc 2018 Dec 10. Epub 2018 Dec 10.

Department of Orthopaedic Surgery, Nippon Medical School, 1-1-5 Sendagi, Bunkyo-ku, Tokyo, 113-8603, Japan.

Purpose: Flexion-type Salter-Harris (SH) II fractures of the proximal tibia, also described as Watson-Jones (WJ) IV fractures, are rare injuries reported among adolescent athletes who are close to skeletal maturity and remain elusive. Due to this classification disagreement, the various treatments range from nonoperative to operative types, and no previous studies have explained the mechanisms of injury or the source of the fracture forces. This retrospective matched case-control study aimed to identify radiological factors that are associated with the fracture forces and to elucidate the mechanisms of these injuries. Read More

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December 2018

A unique nanoparticulate TLR9 agonist enables a HA split vaccine to confer FcγR-mediated protection against heterologous lethal influenza virus infection.

Int Immunol 2018 Dec 7. Epub 2018 Dec 7.

Laboratory of Adjuvant Innovation, Center for Vaccine and Adjuvant Research, Osaka, Japan.

The development of a universal influenza vaccine that can provide a robust and long-lasting protection against a broader range of influenza virus strains is a global public health priority. One approach to improve vaccine efficacy is to use an adjuvant to boost immune responses to the target antigens; nevertheless, the role of adjuvants in the context of influenza vaccines is not fully understood. We have previously developed the K3-schizophyllan (SPG) adjuvant, which is composed of nanoparticulated oligodeoxynucleotides K3, a TLR9 agonist, with SPG, a non-agonistic β-glucan ligand of Dectin-1. Read More

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December 2018

Highly Efficient Ultracentrifugation-free Chromatographic Purification of Recombinant AAV Serotype 9.

Mol Ther Methods Clin Dev 2018 Dec 1;11:180-190. Epub 2018 Nov 1.

Department of Biochemistry and Molecular Biology, Nippon Medical School, Tokyo, Japan.

Recombinant adeno-associated virus serotype 9 (rAAV9) can specifically transduce muscle and neuronal tissues; thus, rAAV9 can potentially be used in gene therapy. However, rAAV9 is the most challenging rAAV serotype to purify. Traditionally, rAAV9 has been purified by ultracentrifugation, which is not scalable. Read More

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December 2018

Accelerometric outcomes of motor function related to clinical evaluations and muscle involvement in dystrophic dogs.

PLoS One 2018 11;13(12):e0208415. Epub 2018 Dec 11.

Department of Molecular Therapy, National Institute of Neuroscience, National Center of Neurology and Psychiatry, Kodaira, Tokyo, Japan.

Duchenne muscular dystrophy (DMD) is an X-linked muscle disorder characterized by primary muscle degeneration. Patients with DMD reveal progressive muscle weakness leading to ambulatory dysfunction. Novel outcome measures are needed for more sensitive evaluation of therapeutic effects in clinical trials. Read More

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December 2018
1 Read

Interstitial Lung Disease Onset and Its Risk Factors in Japanese Patients with ALK-positive NSCLC Following Treatment with Crizotinib.

J Thorac Oncol 2018 Dec 3. Epub 2018 Dec 3.

Tokyo Medical University Hospital, 6-7-1 Nishishinjuku, Shinjuku-ku, Tokyo 160-0023, Japan.

Introduction: The study objective was to determine the incidence and characteristics of drug-induced interstitial lung disease (ILD) associated with an orally available small-molecule tyrosine kinase inhibitor, crizotinib in the real-world clinical setting.

Methods: Post-marketing surveillance was performed in Japan to obtain information on the safety and efficacy of crizotinib. Target patients included all patients with anaplastic lymphoma kinase-positive non-small cell lung cancer (NSCLC) who received crizotinib, during the enrollment period between May 2012 and December 2014. Read More

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December 2018
5 Reads

Synthesis and biochemical characterization of quasi-stable trimer models of full-length amyloid β40 with a toxic conformation.

Chem Commun (Camb) 2018 Dec 6. Epub 2018 Dec 6.

Division of Food Science and Biotechnology, Graduate School of Agriculture, Kyoto University, Kyoto 606-8502, Japan.

Here, we report the first synthesis of quasi-stable trimer models of full-length Aβ40 with a toxic conformation using a 1,3,5-phenyltris-l-alanyl linker at position 34, 36, or 38. The only trimer to exhibit weak neurotoxicity against SH-SY5Y cells was the one which was linked at position 38. This suggests that such a propeller-type trimer model is not prone to forming oligomers with potent neurotoxicity, which is in contrast with its corresponding dimer model. Read More

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December 2018
1 Read

Murine -linked hydrocephalus is caused by hyperpermeability of the choroid plexus.

EMBO Mol Med 2018 Dec 5. Epub 2018 Dec 5.

Cardeza Center for Vascular Biology, Sidney Kimmel Medical College, Thomas Jefferson University, Philadelphia, PA, USA

Though congenital hydrocephalus is heritable, it has been linked only to eight genes, one of which is Humans and mice that carry a truncated version of MPDZ incur severe hydrocephalus resulting in acute morbidity and lethality. We show by magnetic resonance imaging that contrast medium penetrates into the brain ventricles of mice carrying a loss-of-function mutation, whereas none is detected in the ventricles of normal mice, implying that the permeability of the choroid plexus epithelial cell monolayer is abnormally high. Comparative proteomic analysis of the cerebrospinal fluid of normal and hydrocephalic mice revealed up to a 53-fold increase in protein concentration, suggesting that transcytosis through the choroid plexus epithelial cells of KO mice is substantially higher than in normal mice. Read More

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December 2018
2 Reads

Interstitial lung disease associated with nanoparticle albumin-bound paclitaxel treatment in patients with lung cancer.

Jpn J Clin Oncol 2018 Dec 1. Epub 2018 Dec 1.

Department of Pulmonary Medicine and Oncology, Graduate School of Medicine, Nippon Medical School, Tokyo, Japan.

Background: Nanoparticle albumin-bound paclitaxel is indicated for the treatment of patients with lung cancer. It can induce interstitial lung disease, but the incidence of nanoparticle albumin-bound paclitaxel-associated interstitial lung disease in clinical practice has not been determined. We investigated the incidence of interstitial lung disease in patients with lung cancer who had received nanoparticle albumin-bound paclitaxel therapy at our institution. Read More

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December 2018

Preliminary Clinical Microneurosurgical Experience With the 4K3-Dimensional Microvideoscope (ORBEYE) System for Microneurological Surgery: Observation Study.

Oper Neurosurg (Hagerstown) 2018 Dec 3. Epub 2018 Dec 3.

Department of Neurological Surgery, Nippon Medical School, Tokyo, Japan.

Background: The exoscope has been reported as a novel neurosurgical instrumentation in clinical practice.

Objective: To investigate the possibility that ORBEYE (OE), a novel instrument that excludes eyepiece lenses and allows for microsurgery by observation of the 4K3D monitor, could replace microscopes.

Methods: We report 22 clinical cases by 5 experienced neurosurgeons and the comparative results of training 10 residents. Read More

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December 2018

Obesity and adiposity indicators in asthma and allergic rhinitis in children.

Curr Opin Allergy Clin Immunol 2018 Nov 29. Epub 2018 Nov 29.

Department of Pediatrics, Nippon Medical School, Kanagawa, Tokyo.

Purpose Of Review: The prevalence of obesity and allergic diseases, such as asthma and allergic rhinitis, is increasing worldwide not only in adults, but also in children. Experimental and clinical studies have demonstrated the effect of obesity not only on asthma, but also on other allergic diseases.

Recent Findings: Allergic diseases, such as asthma and allergic rhinitis, are common chronic inflammatory diseases of the airways. Read More

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November 2018

Evaluation of Space-Maintaining Sinus Membrane Using the Absorbable Screws in Sinus Lifting Bone Augmentation.

Implant Dent 2018 Nov 30. Epub 2018 Nov 30.

Professor, Division of Clinical Medicine, Department of Oral and Maxillofacial Surgery, Graduate Faculty of Interdisciplinary, University of Yamanashi, Yamanashi, Japan.

Purpose: We investigated whether it was possible to perform a sinus lift maintaining absorbable screw material with biological activity.

Materials And Methods: Thirty rabbits were used. The screws used in this study were unsintered hydroxyapatite/poly-L-lactic acid (uHA/PLLA) (uHA/PLLA group) and titanium alloy (Ti group). Read More

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November 2018

Nippon AMR One Health Report: the first step towards multisectoral collaboration.

Lancet Infect Dis 2018 Nov 24;18(11):1179-1180. Epub 2018 Oct 24.

Infectious Disease Control Division, Health Service Bureau, Ministry of Health, Labour and Welfare, Tokyo 100-8916, Japan.

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November 2018

Vascular endothelial growth factor signaling in VE-cadherin expression and tube-like formation by rheumatoid arthritic synovial fibroblast-like cells.

Biochem Biophys Res Commun 2018 Nov 28. Epub 2018 Nov 28.

Department of Biochemistry, The Nippon Dental University School of Life Dentistry at Tokyo, 1-9-20 Fujimi, Chiyoda-ku, Tokyo, 102-8159, Japan. Electronic address:

An increase in the vasculature is one of most representative changes in the synovial tissue of joints in rheumatoid arthritis (RA) and is closely associated with disease progression. Although the vasculatures are believed to be a result of VE-cadherin-dependent angiogenesis and a possible therapeutic target of the disease, synovial fibroblastic cells express VE-cadherin and form tube-like structures, suggesting that vasculatures in RA synovium may not simply result from angiogenesis. This paper analyzes a mechanism of VE-cadherin expression by rheumatoid arthritic synovial fibroblast-like cells (RSFLs) and their involvement in the tube-like formation. Read More

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November 2018

Oxytocin is indispensable for conspecific-odor preference and controls the initiation of female, but not male, sexual behavior in mice.

Neurosci Res 2018 Nov 28. Epub 2018 Nov 28.

Department of Physiology, Nippon Medical School, Tokyo, Japan; Department of Animal Sciences, Teikyo University of Science, Yamanashi, Japan. Electronic address:

Oxytocin (OT) has been demonstrated to be involved in various social behaviors in mammals. However, OT gene knockout (OTKO) mice can conceive and deliver successfully, though females cannot rear their pups because of lack of lactation. Here, we investigated the sociosexual behavior of both sexes in two experimental setups: olfactory preference for sexual partner's odor and direct social interaction in an enriched condition. Read More

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November 2018

Intestinal absorption of black chokeberry cyanidin 3-glycosides is promoted by capsaicin and capsiate in a rat ligated small intestinal loop model.

Food Chem 2019 Mar 22;277:323-326. Epub 2018 Oct 22.

Sapporo Anti-Aging Laboratory Co., Ltd., Sapporo 061-3204, Japan; Hokkaido Food and Health Research Institute, Sapporo 004-0863, Japan.

Recent studies have shown that aronia (black chokeberry) and haskap fruits (contain anthocyanins) have beneficial health effects in animals and humans. However, some reports have shown that anthocyanin is poorly absorbed in the small intestine. In this study, we compared the intestinal absorption of aronia and haskap anthocyanins by using rats with a ligated small intestinal loop and cannulated portal vein. Read More

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March 2019
1 Read

Pharmacokinetic and pharmacodynamic studies of pregabalin suppositories based on pharmacological research.

J Pharm Pharmacol 2018 Dec 4. Epub 2018 Dec 4.

Pharmaceutical Common Achievement Tests Organization, Tokyo, Japan.

Objectives: As commercially available pregabalin preparations are limited to oral administration, it is impossible to use it as an adjuvant analgesic for neuropathic cancer-related pain in terminally ill cancer patients with oral feeding difficulties. The objective of this study was to develop a pregabalin suppository to be available at hospitals.

Methods: Pregabalin suppositories were prepared using bases comprising six different compositions of Witepsol H-15, Witepsol S-55, and Witepsol E-75. Read More

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December 2018

Benralizumab does not impair antibody response to seasonal influenza vaccination in adolescent and young adult patients with moderate to severe asthma: results from the Phase IIIb ALIZE trial.

J Asthma Allergy 2018 20;11:181-192. Epub 2018 Nov 20.

AstraZeneca, Gaithersburg, MD, USA.

Background And Objectives: Benralizumab is a humanized, afucosylated monoclonal antibody against the IL-5Rα. Initial monthly followed by every-other-month injections result in rapid and nearly complete eosinophil depletion. We evaluated whether three doses of benralizumab modifies antibody response to seasonal influenza vaccination for adolescent/young adult patients with moderate to severe asthma. Read More

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November 2018

Efficacy and safety of alirocumab 150mg every 4 weeks in hypercholesterolemic patients on non-statin lipid-lowering therapy or lowest strength dose of statin: ODYSSEY NIPPON.

J Cardiol 2018 Nov 30. Epub 2018 Nov 30.

Department of Cardiovascular Medicine, Institute of Biomedical Sciences, Tokushima University Graduate School, Tokushima, Japan.

Background: Alirocumab, a fully human monoclonal antibody to proprotein convertase subtilisin/kexin type 9, given every 2 weeks (Q2W), significantly reduced low-density lipoprotein cholesterol (LDL-C) levels in Japanese hypercholesterolemic patients on background statin. We evaluated alirocumab 150mg every 4 weeks (Q4W) in patients on lowest-dose statin or non-statin lipid-lowering therapy (LLT).

Methods: ODYSSEY NIPPON was a double-blind study conducted in Japanese patients with LDL-C ≥100mg/dL (heterozygous familial hypercholesterolemia or non-familial hypercholesterolemia with coronary heart disease) or ≥120mg/dL (non-familial hypercholesterolemia, Japan Atherosclerosis Society category III) on atorvastatin 5mg/day or non-statin LLT. Read More

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November 2018

Identification of velvet antler and its mixed varieties by UPLC-QTOF-MS combined with principal component analysis.

J Pharm Biomed Anal 2018 Oct 4;165:18-23. Epub 2018 Oct 4.

Research and Inspection Center of Traditional Chinese Medicine and Ethnomedicine, National Institutes for Food and Drug Control, State Food and Drug Administration, 2 Tiantan Xili, Beijing, 100050, China. Electronic address:

Many species of velvet antler have been used as traditional medicine for thousands of years; however, as medicinal materials, velvet antler derived from different animals have different clinical effects. To distinguish the differences and homologies, ultra-performance liquid chromatography-quadruple-time of flight mass spectrometry (UPLC-QTOF-MS) coupled with principal component analysis (PCA) was developed and applied to identify these antler samples derived from Cervus nippon Temminck, Cervus elaphus Linnaeus and Rangifer tarandus Linnaeus, which were first tested and compared at the molecular level of protein. The UPLC-MS data of the trypsin digested samples were subjected to PCA, and the potential markers based on peptide were depicted to illustrate their differences. Read More

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October 2018

Layer-specific myocardial function in asymptomatic cats with obstructive hypertrophic cardiomyopathy assessed using 2-dimensional speckle-tracking echocardiography.

J Vet Intern Med 2018 Nov 29. Epub 2018 Nov 29.

Laboratory of Veterinary Internal Medicine, Division of Therapeutic Sciences 1, Department of Veterinary Clinical Medicine, Faculty of Veterinary Medicine, Nippon Veterinary and Life Science University, Tokyo, Japan.

Background: Hypertrophic cardiomyopathy (HCM), a primary disorder of the myocardium, is the most common cardiac disease in cats. However, determination of layer-specific myocardial function with 2D speckle-tracking echocardiography in cats with asymptomatic HCM has not yet been reported.

Objectives: To quantitatively measure layer-specific myocardial function of asymptomatic cats with HCM. Read More

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November 2018
1 Read

Brachial-ankle pulse wave velocity, cardio-ankle vascular index, and prognosis.

Authors:
Dai Ato

Vasc Health Risk Manag 2018 24;14:321-348. Epub 2018 Oct 24.

Gakujutsu Shien Co., Ltd, Tokyo, Japan,

Background: Brachial-ankle pulse wave velocity (baPWV) and cardio-ankle vascular index (CAVI) are indices of arterial stiffness, and several studies have used these indices. However, there is no comprehensive review of these parameters in the prognostic significance.

Methods: The aim of this study was to review the articles exploring the prognostic significance of these parameters. Read More

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October 2018
1 Read

Genetic determinants and an epistasis of and HLA-B*52 in Takayasu arteritis.

Proc Natl Acad Sci U S A 2018 Nov 29. Epub 2018 Nov 29.

Center for Genomic Medicine, Kyoto University Graduate School of Medicine, Kyoto 606-8507, Japan.

Takayasu arteritis (TAK) is a systemic vasculitis with severe complications that affects the aorta and its large branches. HLA-B*52 is an established susceptibility locus to TAK. To date, there are still only a limited number of reports concerning non-HLA susceptibility loci to TAK. Read More

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November 2018
2 Reads

Identification of RNA biomarkers for chemical safety screening in neural cells derived from mouse embryonic stem cells using RNA deep sequencing analysis.

Biochem Biophys Res Commun 2018 Nov 26. Epub 2018 Nov 26.

Department of Electrical Engineering and Bioscience, Faculty of Science and Engineering, Waseda University, 55N-06-10, 3-4-1 Okubo, Shinjuku-ku, Tokyo, 169-8555, Japan; AIST-Waseda University Computational Bio Big-Data Open Innovation Laboratory (CBBD-OIL), 3-4-1 Okubo, Shinjuku-ku, Tokyo, 169-8555, Japan; Artificial Intelligence Research Center, National Institute of Advanced Industrial Science and Technology (AIST), 2-41-6 Aomi, Koto-ku, Tokyo, 135-0064, Japan; Institute for Medical-oriented Structural Biology, Waseda University, 2-2 Wakamatsu-cho, Shinjuku-ku, Tokyo, 162-8480, Japan; Graduate School of Medicine, Nippon Medical School, 1-1-5 Sendagi, Bunkyo-ku, Tokyo, 113-8602, Japan.

For chemical safety screening, there is a need for more efficient assays that do not involve animals. Among the alternatives to animal testing, in vitro cell-based assays are among the most appropriate to screen the toxicity of chemicals. Previous studies focused on mRNAs as biomarkers; however, recent whole-transcriptome analyses have revealed the presence of a large number of unannotated noncoding RNAs (ncRNAs) that appear not to have any protein-coding potential. Read More

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November 2018
3 Reads

NLRX1 Negatively Regulates Group A Invasion and Autophagy Induction by Interacting With the Beclin 1-UVRAG Complex.

Front Cell Infect Microbiol 2018 14;8:403. Epub 2018 Nov 14.

Department of Microbiology, Graduate School of Medicine, Kyoto University, Kyoto, Japan.

Group A (GAS) can invade epithelial cells; however, these bacteria are targeted and eventually destroyed by autophagy. Members of the Nod-like receptor (NLR) family are thought to be critical for the autophagic response to invasive bacteria. However, the intracellular sensors within host cells that are responsible for bacterial invasion and the induction of autophagy are largely unknown. Read More

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November 2018

Carotid surgical cut-down technique for neuroendovascular therapy.

Interv Neuroradiol 2018 Nov 28:1591019918815920. Epub 2018 Nov 28.

1 Department of Neurosurgery, Dokkyo Medical University, Saitama Medical Center, Japan.

Background: Neuroendovascular therapy is typically performed via the femoral artery, but there are rare cases in which a tortuous upstream angioarchitecture makes it difficult to access the intracranial circulation via this route.

Methods: In this case series, we describe six cases treated by surgical cut-down in the neck, with puncture of the carotid artery. Antiplatelet and anticoagulation agents were used in all cases. Read More

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November 2018
5 Reads

Efficacy and safety of ombitasvir/paritaprevir/ritonavir and ribavirin for chronic hepatitis patients infected with genotype 2a in Japan.

Hepatol Res 2018 Nov 28. Epub 2018 Nov 28.

Division of Gastroenterology and Hepatology, Department of Internal Medicine, Nippon Medical School, Tokyo, Japan.

Aim: The aim of this study was to evaluate the efficacy and safety of community-based ombitasvir/paritaprevir/ritonavir plus ribavirin therapy for non-cirrhotic patients with hepatitis C virus (HCV) genotype 2a infection in a real-world setting.

Methods: Patients with HCV genotype 2a infection were enrolled in this study and received the therapy for 16 weeks at 11 specialized centers between October 2016 and July 2017. Among the 98 patients participating in the study, 4 patients were excluded because of the presence of cirrhosis and/or genotype 2b infection. Read More

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November 2018
1 Read

Retrograde superselective intra-arterial chemoradiotherapy combined with hyperthermia and cetuximab for carcinoma of the buccal mucosa with N3 lymph node metastasis: a case report.

Oral Radiol 2018 Feb 5. Epub 2018 Feb 5.

Department of Oral and Maxillofacial Surgery, Yokohama City University Graduate School of Medicine, 3-9 Fukuura, Kanazawa-ku, Yokohama, 236-0004, Japan.

We herein report a case of squamous cell carcinoma of the buccal mucosa with N3 cervical lymph node metastasis in a 63-year-old man. The patient was treated with combination therapy comprising radiotherapy (2 Gy/day, total of 70 Gy), superselective intra-arterial chemotherapy via a superficial temporal artery (docetaxel, total of 70 mg/m and cisplatin, total of 175 mg/m), cetuximab (initial dose of 400 mg/m with subsequent weekly doses of 250 mg/m intravenously), and four sessions of hyperthermia for cervical lymph node metastases. The patient responded well to the therapy, with a complete response of the primary tumor. Read More

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February 2018

Evaluation of permanent and primary enamel and dentin mineral density using micro-computed tomography.

Oral Radiol 2018 Jan 16. Epub 2018 Jan 16.

Division of Oral and Maxillofacial Radiology, Niigata University Graduate School of Medical and Dental Sciences, 5274 Gakkocho-dori, Chuo-ku, Niigata, 951-8514, Japan.

Objectives: The present study was performed to investigate the mineral density distribution in enamel and dentin for both permanent and primary teeth and to establish the standard density per tooth type using micro-computed tomography (CT).

Methods: Fifty-seven extracted human teeth (37 permanent, 20 primary) were evaluated in the present study. The enamel and dentin mineral densities in the extracted teeth were measured using micro-CT. Read More

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January 2018
1 Read

Clinical guidelines for dental cone-beam computed tomography.

Oral Radiol 2018 May 11;34(2):89-104. Epub 2018 Jan 11.

Oral and Maxillofacial Radiology, Applied Oral Sciences, Faculty of Dentistry, The University of Hong Kong, Prince Philip Dental Hospital, 34 Hospital Road, Sai Ying Pun, Hong Kong, SAR, China.

Dental cone-beam computed tomography (CBCT) received regulatory approval in Japan in 2000 and has been widely used since being approved for coverage by the National Health Insurance system in 2012. This imaging technique allows dental practitioners to observe and diagnose lesions in the dental hard tissue in three dimensions (3D). When performing routine radiography, the examination must be justified, and optimal protection should be provided according to the ALARA (as low as reasonably achievable) principles laid down by the International Commission on Radiological Protection. Read More

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May 2018
8 Reads

Cone-beam computed tomography and anatomical observations of normal variants in the mandible: variant dentists should recognize.

Oral Radiol 2018 Sep 18;34(3):189-198. Epub 2017 Nov 18.

Department of Oral and Maxillofacial Radiology, School of Life Dentistry at Tokyo, Nippon Dental University, 1-9-20, Fujimi Chiyoda-ku, Tokyo, 102-8159, Japan.

Many types of cone-beam computed tomography (CBCT) have recently been developed and are being used in many dental clinics. CBCT has significantly changed imaging diagnosis in dentistry by showing detailed structures in multiple dimensions with high spatial resolution. This allows dentists to confirm what cannot be recognized with conventional two-dimensional images, such as small neurovascular canals, the buccolingual location of structures/lesions, and the three-dimensional shapes of hard tissue structures. Read More

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September 2018
1 Read

Diffusion-weighted magnetic resonance imaging in oral and maxillofacial lesions: preliminary study on diagnostic ability of apparent diffusion coefficient maps.

Oral Radiol 2018 Sep 4;34(3):224-228. Epub 2017 Sep 4.

Department of Radiology, The Nippon Dental University Niigata Hospital, 1-8 Hamaura-cho, Chuo-ku, Niigata, Niigata, 951-8580, Japan.

Objectives: The aim of this study was to investigate the usefulness of diffusion-weighted magnetic resonance imaging (DWI) in oral and maxillofacial lesions, especially the utility of apparent diffusion coefficient (ADC) maps for differential diagnosis of these lesions.

Methods: Fifty-seven patients who underwent magnetic resonance imaging for diagnosis of oral and maxillofacial lesions were included in this prospective study. DWI was performed on a 1. Read More

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September 2018

Age-related changes in the pulp chamber of maxillary and mandibular molars on cone-beam computed tomography images.

Oral Radiol 2018 Sep 7;34(3):219-223. Epub 2017 Aug 7.

Radiology, The Nippon Dental University Niigata Hospital, 1-8 Hamaura-cho, Chuo-ku, Niigata, Niigata, 951-8580, Japan.

Objectives: Secondary dentine is laid on pulp chamber walls with increasing age, and decreases pulp chamber size. This study aimed to investigate age estimation on cone-beam computed tomography (CBCT) images for forensic science, and the relationship between age and pulp chamber area of maxillary and mandibular molars.

Methods: We reviewed the CBCT images of 316 first molars in 87 patients with dental lesions. Read More

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September 2018

The Effect of Linagliptin versus Metformin Treatment-Related Quality of Life in Patients with Type 2 Diabetes Mellitus.

Diabetes Ther 2018 Nov 27. Epub 2018 Nov 27.

Department of Metabolism and Endocrinology, Juntendo University Graduate School of Medicine, Bunkyo-ku, Tokyo, Japan.

Introduction: There have been no studies directly comparing the effect of dipeptidyl peptidase-4 inhibitors with that of metformin on treatment-related quality of life (QOL) when used as first-line therapy in patients with type 2 diabetes mellitus (T2DM).

Methods: This study is a prospective, randomized, open-label, multicenter, parallel-group, comparative study. Forty-four participants who failed to achieve target glycemic control with diet and exercise therapy were randomly allocated to receive linagliptin or metformin therapy. Read More

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November 2018

CXCL12 regulates differentiation of human immature melanocyte precursors as well as their migration.

Arch Dermatol Res 2018 Nov 27. Epub 2018 Nov 27.

Department of Applied Cell and Regenerative Medicine, Fujita Health University School of Medicine, 1-98 Dengakugakubo, Kutsukakecho, Toyoake, Aichi, Japan.

Melanocyte stem cells (McSCs) are localized in the bulge region of hair follicles and supply melanocytes, which determine hair color by synthesizing melanin. Ectopic differentiation of McSCs, which are usually undifferentiated in the bulge region, causes depletion of McSCs and results in hair graying. Therefore, to prevent hair graying, it is essential to maintain McSCs in the bulge region, but the mechanism of McSC maintenance remains unclear. Read More

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November 2018
1 Read

Kinetic modeling and sensitivity analysis for higher ethanol production in self-cloning xylose-using Saccharomyces cerevisiae.

J Biosci Bioeng 2018 Nov 24. Epub 2018 Nov 24.

Laboratory of Synthetic Biology, Graduate School of Systems Life Sciences, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka 812-8582, Japan. Electronic address:

We constructed a xylose-utilizing Saccharomyces cerevisiae strain using endogenous xylose-assimilating genes (strain K7-XYL). Such self-cloning yeast is expected to make a great contribution to cost reduction of ethanol production processes. However, it is difficult to modify self-cloning yeast for optimal performance because the available gene source is limited. Read More

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November 2018
3 Reads

Isolation and characterization of cells derived from human epithelial rests of Malassez.

Odontology 2018 Nov 26. Epub 2018 Nov 26.

Department of Endodontics, The Nippon Dental University School of Life Dentistry at Niigata, 1-8 Hamaura-cho, Chuo-ku, Niigata, 951-8580, Japan.

The epithelial rests of Malassez (ERMs) might represent a valuable source of oral epithelial cells with stem cell properties. The purpose of this study was to isolate and characterize cells derived from human ERM, and compare them with cells derived from matched normal oral mucosa (NOM). Matched tissue specimens of the periodontal ligament of extracted tooth and NOM were collected. Read More

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November 2018

Infection of newly identified phleboviruses in ticks and wild animals in Hokkaido, Japan indicating tick-borne life cycles.

Ticks Tick Borne Dis 2018 Nov 16. Epub 2018 Nov 16.

Division of Molecular Pathobiology, Research Center for Zoonosis Control, Hokkaido University, Sapporo, Japan; Global Institution for Collaborative Research and Education (GI-CoRE), Hokkaido University, Sapporo, Japan; Department of Disease Control, School of Veterinary Medicine, University of Zambia, Lusaka, Zambia; Global Virus Network, Baltimore, USA.

Recent discoveries of tick-borne pathogens have raised public health concerns on tick-borne infectious diseases and emphasize the need to assess potential risks of unrecognized tick-borne pathogens. First, to determine the existence of tick-borne phleboviruses (TBPVs), genetic surveillance of phleboviruses in ticks was conducted mainly in Hokkaido, the northernmost island in Japan from 2013 to 2015. Genes of two TBPVs, previously reported as Mukawa virus (MKWV) and a newly identified relative of MKWV, Kuriyama virus (KURV), were detected and the viruses were isolated from Ixodes persulcatus collected in Hokkaido, but not in I. Read More

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November 2018
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Comparative effectiveness and safety of warfarin and dabigatran in patients with non-valvular atrial fibrillation in Japan: A claims database analysis.

J Cardiol 2018 Nov 23. Epub 2018 Nov 23.

Keio University, Faculty of Pharmacology, Tokyo, Japan.

Background: Antithrombotic therapy, including direct oral anticoagulants, is recommended in patients with non-valvular atrial fibrillation (NVAF) who are at intermediate-to-high risk of stroke. The aims of this study were to assess the patterns of oral anticoagulant (OAC) prescription in Japanese patients with NVAF and compare the effectiveness and safety of dabigatran and warfarin.

Methods: This was a retrospective observational study of adults with NVAF who initiated dabigatran or warfarin between March 14, 2011 and June 30, 2016, using electronic claims data of approximately 12. Read More

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November 2018
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Chemical characterization of the milk oligosaccharides of some Artiodactyla species including giraffe (Giraffa camelopardalis), sitatunga (Tragelaphus spekii), deer (Cervus nippon yesoensis) and water buffalo (Bubalus bubalis).

Glycoconj J 2018 12 22;35(6):561-574. Epub 2018 Nov 22.

Department of Life and Food Science, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido, 080-8555, Japan.

Mammalian milk/colostrum usually contains oligosaccharides along with the predominant disaccharide lactose. It has been found that the number and identity of these milk oligosaccharides varies among mammalian species. Oligosaccharides predominate over lactose in the milk/colostrum of Arctoidea species (Carnivora), whereas lactose predominates over milk oligosaccharides in Artiodactyla including cow, sheep, goat, camel, reindeer and pig. Read More

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December 2018

Serum bilirubin concentrations, type 2 diabetes and incident coronary heart disease.

Authors:
Tomoyuki Kawada

Acta Diabetol 2018 Nov 22. Epub 2018 Nov 22.

Department of Hygiene and Public Health, Nippon Medical School, 1-1-5 Sendagi, Bunkyo-Ku, Tokyo, 113-8602, Japan.

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November 2018
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Mitochondrial dysfunction causes hyperexcitability of serotonergic neurons.

Mol Psychiatry 2018 Oct;23(10):1971

Laboratory for Molecular Dynamics of Mental Disorders, RIKEN Center for Brain Science, Wako, Saitama, Japan.

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October 2018
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Characteristics, management, and in-hospital mortality among patients with severe sepsis in intensive care units in Japan: the FORECAST study.

Crit Care 2018 Nov 22;22(1):322. Epub 2018 Nov 22.

Division of Acute and Critical Care Medicine, Hokkaido University Graduate School of Medicine, Sapporo, Japan.

Background: Sepsis is a leading cause of death and long-term disability in developed countries. A comprehensive report on the incidence, clinical characteristics, and evolving management of sepsis is important. Thus, this study aimed to evaluate the characteristics, management, and outcomes of patients with severe sepsis in Japan. Read More

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November 2018
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