1,072 results match your criteria New Biotechnology [Journal]


Pichia pastoris as a host for production and isolation of mutagenic AID/APOBEC enzymes involved in cancer and immunity.

N Biotechnol 2019 Feb 27;51:67-79. Epub 2019 Feb 27.

Immunology and Infectious Diseases Program, Division of Biomedical Sciences, Faculty of Medicine, Memorial University of Newfoundland, Canada. Electronic address:

AID/APOBEC3 enzymes are cytidine deaminases that mutate antibody and retroviral genes and also mediate extensive tumor genome mutagenesis. The study of purified AID/APOBEC3 proteins is challenged by difficulties with their expression and purification arising from genotoxicity in expression hosts, extensive non-specific protein-protein/DNA/RNA interactions and haphazard oligomerization. To date, expression hosts for purification of AID/APOBEC3 enzymes include bacteria, insect and mammalian cells. Read More

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http://dx.doi.org/10.1016/j.nbt.2019.02.006DOI Listing
February 2019
1 Read

The presence of trace components significantly broadens the molecular response of Aspergillus niger to guar gum.

N Biotechnol 2019 Feb 20;51:57-66. Epub 2019 Feb 20.

Fungal Physiology, Westerdijk Fungal Biodiversity Institute, Fungal Molecular Physiology, Utrecht University, Uppsalalaan 8, 3584 CT, Utrecht, the Netherlands; Microbiology, Utrecht University, Padualaan 8, 3584, CH, Utrecht, the Netherlands. Electronic address:

Guar gum consists mainly of galactomannan and constitutes the endosperm of guar seeds that acts as a reserve polysaccharide for germination. Due to its molecular structure and physical properties, this biopolymer has been considered as one of the most important and widely used gums in industry. However, for many of these applications this (hemi-)cellulosic structure needs to be modified or (partially) depolymerized in order to customize and improve its physicochemical properties. Read More

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http://dx.doi.org/10.1016/j.nbt.2019.02.005DOI Listing
February 2019

Cost-effective production of recombinant peptides in Escherichia coli.

N Biotechnol 2019 Feb 18;51:39-48. Epub 2019 Feb 18.

Department of Chemical Sciences, University of Naples Federico II, 80126, Naples, Italy; Istituto Nazionale di Biostrutture e Biosistemi (INBB), Italy. Electronic address:

Among bioactive peptides, cationic antimicrobial peptides (AMPs), also referred to as host defence peptides (HDPs), are valuable tools to treat infections, being able to kill a wide variety of microbes directly and/or modulate host immunity. HDPs have great therapeutic potential against antibiotic-resistant bacteria, viruses and even parasites. However, high manufacturing costs have greatly limited their development as drugs, thus highlighting the need to develop novel and competitive production strategies. Read More

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http://dx.doi.org/10.1016/j.nbt.2019.02.004DOI Listing
February 2019
1 Read

Certain new plant breeding techniques and their marketability in the context of EU GMO legislation - recent developments.

N Biotechnol 2019 Feb 16;51:49-56. Epub 2019 Feb 16.

Institute of Bioorganic Chemistry, Polish Academy of Sciences, Poznań, Poland. Electronic address:

The comparatively low adoption rate of GMO products in the European Union (EU) market seems to be connected with the strictness of authorization regulations and inefficiency of the authorization process itself. These problems will apply to any product deemed to be a GMO that could potentially be marketable in the EU. Since modern methods of plant breeding involving oligonucleotide-directed mutagenesis (ODMs) or site-directed nucleases (SDNs), including Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR), are becoming ever more popular, it is crucial to establish whether the products of such new breeding techniques (NBTs), in particular those which involve precise methods of mutagenesis, are exempted from the EU legislation on GMOs or not. Read More

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http://dx.doi.org/10.1016/j.nbt.2019.02.003DOI Listing
February 2019
1 Read

Biofilm dynamics and EPS production of a thermoacidophilic bioleaching archaeon.

N Biotechnol 2019 Feb 8;51:21-30. Epub 2019 Feb 8.

Biofilm Centre, Universität Duisburg-Essen, Universitätsstraße 5, 45141 Essen, Germany; College of Environmental Science and Engineering, Donghua University, 201620 Shanghai, China; TU Bergakademie Freiberg, Leipziger Straße 29, 09599 Freiberg, Germany. Electronic address:

Bioleaching of metal sulfides represents an interfacial process where biofilm formation is important in the initial steps of this process. In technical applications of bioleaching, such as reactor leaching in the temperature range of 50 up to 90 °C and also in (self-heating) heaps, thermophilic archaea play an important role and often are the leaching organisms of choice. Nevertheless, to date there is little information available on the interactions between thermoacidophilic archaea and their natural mineral substrates such as pyrite. Read More

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http://dx.doi.org/10.1016/j.nbt.2019.02.002DOI Listing
February 2019
1 Read

Using agro-industrial wastes for mixotrophic growth and lipids production by the green microalga Chlorella sorokiniana.

N Biotechnol 2019 Feb 8;51:31-38. Epub 2019 Feb 8.

Center for Marine and Environmental Research - CIMA, University of Algarve - Campus de Gambelas, 8005-139 Faro, Portugal. Electronic address:

There has been growing interest in the use of microalgae for the production of biofuels, but production costs continue to be too high to compete with fossil fuel prices. One of the main limitations for photobioreactor productivity is light shielding, especially at high cell densities. The growth of the green microalga Chlorella sorokiniana, a robust industrial species, has been evaluated under different trophic conditions with traditional carbon sources, such as glucose and sucrose, and alternative low cost carbon sources, such as carob pod extract, industrial glycerol and acetate-rich oxidized wine waste lees. Read More

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http://dx.doi.org/10.1016/j.nbt.2019.02.001DOI Listing
February 2019

Microbial production of high value molecules using rayon waste material as carbon-source.

N Biotechnol 2019 Feb 1;51:8-13. Epub 2019 Feb 1.

Austrian Centre of Industrial Biotechnology GmbH, Konrad Lorenz Strasse 20, 3430 Tulln an der Donau, Austria; University of Natural Resources and Life Sciences, Department of Biotechnology, Muthgasse 18, 1190 Vienna, Austria.

Rayon filaments composed of regenerated cellulose are used as reinforcement materials in tires and to a lower extent in the clothing industry as personal protective equipment e.g. flame retardant cellulosic based materials. Read More

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http://dx.doi.org/10.1016/j.nbt.2019.01.010DOI Listing
February 2019
2 Reads

Microbial co-culturing strategies for fructo-oligosaccharide production.

N Biotechnol 2019 Jan 29;51:1-7. Epub 2019 Jan 29.

Applied Chemistry and Biochemistry Unit, Faculty of Engineering, University of Mons, 7000 Mons, Belgium.

Fructo-oligosaccharide (FOS) mixtures produced by fermentation contain large amounts of non-prebiotic sugars. Here we propose a mixed culture of Aureobasidium pullulans and Saccharomyces cerevisiae cells to produce FOS and consume the small saccharides simultaneously, thereby increasing FOS purity in the mixture. The use of immobilised A. Read More

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http://dx.doi.org/10.1016/j.nbt.2019.01.009DOI Listing
January 2019

Directed evolution of the type C feruloyl esterase from Fusarium oxysporum FoFaeC and molecular docking analysis of its improved variants.

N Biotechnol 2019 Jan 24;51:14-20. Epub 2019 Jan 24.

Department of Chemical Sciences, University of Naples "Federico II", Via Cintia 4, 80126, Naples, Italy. Electronic address:

The need to develop competitive and eco-friendly processes in the cosmetic industry leads to the search for new enzymes with improved properties for industrial bioconversions in this sector. In the present study, a complete methodology to generate, express and screen diversity for the type C feruloyl esterase from Fusarium oxysporium FoFaeC was set up in a high-throughput fashion. A library of around 30,000 random mutants of FoFaeC was generated by error prone PCR of fofaec cDNA and expressed in Yarrowia lipolytica. Read More

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http://dx.doi.org/10.1016/j.nbt.2019.01.008DOI Listing
January 2019
5 Reads

Production of lignin-modifying enzymes by Trametes ochracea on high-molecular weight fraction of olive mill wastewater, a byproduct of olive oil biorefinery.

N Biotechnol 2019 May 19;50:44-51. Epub 2019 Jan 19.

Department for Innovation in Biological, Agro-food and Forest systems (DIBAF), University of Tuscia, Via San Camillo De Lellis, 01100, Viterbo, Italy.

The high-molecular weight fraction of olive mill wastewater (HMW-OMW), a byproduct of olive oil biorefinery, was used at the reactor level as the basal medium for production of laccase and Mn-dependent peroxidase (MnP) by Trametes ochracea. Three reactor systems, namely stirred tank reactors equipped with either Rushton turbines or marine impeller and draft tube (STR and STR-MD, respectively) and an air-lift reactor (ALR) were compared for this purpose. Although inocula were supplied as intact pellets, in both STR-based systems fungal growth evolved rapidly into a dispersed form while the ALR enabled the maintenance of the pellet growth mode. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S18716784183012
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http://dx.doi.org/10.1016/j.nbt.2019.01.007DOI Listing
May 2019
4 Reads

Glycerol metabolism of Pichia pastoris (Komagataella spp.) characterised by C-based metabolic flux analysis.

N Biotechnol 2019 May 17;50:52-59. Epub 2019 Jan 17.

Department of Chemical, Biological and Environmental Engineering, Universitat Autònoma de Barcelona, Bellaterra, Cerdanyola del Vallès, Catalonia, Spain. Electronic address:

Metabolic flux analysis based on C-derived constraints has proved to be a powerful method for quantitative physiological characterisation of one of the most extensively used microbial cell factory platforms, Pichia pastoris (syn. Komagataella spp.). Read More

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https://linkinghub.elsevier.com/retrieve/pii/S18716784183019
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http://dx.doi.org/10.1016/j.nbt.2019.01.005DOI Listing
May 2019
3 Reads

Hydrocarbonoclastic Ascomycetes to enhance co-composting of total petroleum hydrocarbon (TPH) contaminated dredged sediments and lignocellulosic matrices.

N Biotechnol 2019 May 14;50:27-36. Epub 2019 Jan 14.

Department of Biology, University of Pisa, Pisa, Italy. Electronic address:

Four new Ascomycete fungi capable of degrading diesel oil were isolated from sediments of a river estuary mainly contaminated by shipyard fuels or diesel oil. The isolates were identified as species of Lambertella, Penicillium, Clonostachys, and Mucor. The fungal candidates degraded and adsorbed the diesel oil in suspension cultures. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S18716784183015
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http://dx.doi.org/10.1016/j.nbt.2019.01.006DOI Listing
May 2019
5 Reads

Combination of ribosome display and next generation sequencing as a powerful method for identification of affibody binders against β-lactamase CTX-M15.

N Biotechnol 2019 May 8;50:60-69. Epub 2019 Jan 8.

BIOASTER, 40 Avenue Tony Garnier, 69007 Lyon, France.

CTX-M15 is one of the most widespread, extended spectrum β-lactamases, a major determinant of antibiotic resistance representing urgent public health threats, among enterobacterial strains infecting humans and animals. Here we describe the selection of binders to CTX-M15 from a combinatorial affibody library displayed on ribosomes. Upon three increasingly selective ribosome display iterations, selected variants were identified by next generation sequencing (NGS). Read More

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http://dx.doi.org/10.1016/j.nbt.2019.01.004DOI Listing
May 2019
2 Reads

Application of dissolved oxygen (DO) level control for polyhydroxyalkanoate (PHA) accumulation with concurrent nitrification in surplus municipal activated sludge.

N Biotechnol 2019 May 8;50:37-43. Epub 2019 Jan 8.

UCIBIO (Applied Molecular Biosciences Unit), REQUIMTE (Network of Chemistry and Technology), Chemistry Department, Faculty of Sciences and Technology, Nova University of Lisbon, 2829-516 Caparica, Portugal.

Mixed microbial cultures are a viable means for polyhydroxyalkanoate (PHA) production, which can produce polymers of commercial quality with high yields. Various PHA co-polymer blends can be produced by surplus full-scale municipal activated sludge fed with fermented waste feedstocks. In biological nutrient removal, ammonia is converted to nitrate by ammonia and nitrite oxidizing bacteria (AOBs and NOBs) through nitrification and removed as nitrogen gas through denitrification. Read More

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http://dx.doi.org/10.1016/j.nbt.2019.01.003DOI Listing
May 2019
2 Reads

Impact of temperature and pH on recombinant human IgM quality attributes and productivity.

N Biotechnol 2019 May 7;50:20-26. Epub 2019 Jan 7.

Department of Biotechnology, VIBT, University of Natural Resources and Life Sciences, Vienna, Muthgasse 18, 1190, Vienna, Austria. Electronic address:

IgM antibodies are arousing considerable interest as biopharmaceuticals. Despite their immunotherapeutic potential, little is known about the impact of environmental conditions on product quantity and quality of these complex molecules. Process conditions influence the critical quality attributes (CQAs) of therapeutic proteins and thus are important parameters for biological safety and efficacy. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S18716784183176
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http://dx.doi.org/10.1016/j.nbt.2019.01.001DOI Listing
May 2019
10 Reads

Overexpression of the nitroreductase NfsB in an E. coli strain as a whole-cell biocatalyst for the production of chlorinated analogues of the natural herbicide DIBOA.

N Biotechnol 2019 May 7;50:9-19. Epub 2019 Jan 7.

Department of Biomedicine, Biotechnology and Public Health-Biochemistry and Molecular Biology, University of Cadiz, Campus Universitario de Puerto Real, Universidad de Cádiz, 11510, Puerto Real, Cadiz, Spain. Electronic address:

Benzohydroxamic acids, such as DIBOA (2,4-dihydroxy-2 H)-1,4-benzoxazin-3(4 H)-one), are plant products that exhibit interesting herbicidal, fungicidal and bactericidal properties. A feasible alternative to their purification from natural sources is the synthesis of analogous compounds such as D-DIBOA (2-deoxy-DIBOA) and their chlorinated derivatives. Their chemical synthesis has been simplified into two steps. Read More

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http://dx.doi.org/10.1016/j.nbt.2019.01.002DOI Listing

Physiological characterization of a pyrimidine auxotroph exposes link between uracil phosphoribosyltransferase regulation and riboflavin production in Ashbya gossypii.

N Biotechnol 2019 May 24;50:1-8. Epub 2018 Dec 24.

CEB - Centre of Biological Engineering, University of Minho, 4710-057, Braga, Portugal. Electronic address:

The blockage of the de novo pyrimidine biosynthetic pathway at the orotidine-5'-phosphate decarboxylase level was previously demonstrated to affect riboflavin production in the industrial producer fungus Ashbya gossypii. However, the molecular basis for the unusual sensitivity to uracil displayed by the pyrimidine auxotroph A. gossypii Agura3 was unknown. Read More

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http://dx.doi.org/10.1016/j.nbt.2018.12.004DOI Listing

SpyTag/SpyCatcher molecular cyclization confers protein stability and resilience to aggregation.

N Biotechnol 2019 Mar 17;49:28-36. Epub 2018 Dec 17.

College of Biological and Environmental Sciences, Zhejiang Wanli University, Ningbo 315100, Zhejiang, China. Electronic address:

The capacities for thermal and inhibitor tolerance are critical for industrial enzymes and loss of activity is a major challenge in deploying natural enzymes for commercial applications. Protein engineering approaches, such as site-directed mutagenesis and directed evolution, have been devoted to modifying natural enzymes. Recently, a post-translation protein engineering strategy, the SpyTag/SpyCatcher system, was introduced. Read More

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http://dx.doi.org/10.1016/j.nbt.2018.12.003DOI Listing
March 2019
1 Read
2.898 Impact Factor

A novel thermophilic laccase-like multicopper oxidase from Thermothelomyces thermophila and its application in the oxidative cyclization of 2',3,4-trihydroxychalcone.

N Biotechnol 2019 Mar 5;49:10-18. Epub 2018 Dec 5.

Biotechnology Laboratory, School of Chemical Engineering, National Technical University of Athens, 5 Iroon Polytechniou Str., Zografou Campus, Athens 15780, Greece; Biochemical and Chemical Process Engineering, Division of Sustainable Process Engineering, Department of Civil, Environmental and Natural Resources Engineering, Luleå University of Technology, SE-97187 Luleå, Sweden. Electronic address:

Laccase-like multicopper oxidases (LMCOs) are a heterogeneous group of oxidases, acting mainly on phenolic compounds and which are widespread among many microorganisms, including Basidiomycetes and Ascomycetes. Here, we report the cloning, heterologous expression, purification and characterization of a novel LMCO from the thermophilic fungus Thermothelomyces thermophila. The 1953 bp lmco gene sequence comprises of 3 exons interrupted by 2 introns and according to the LccED database the translated sequence belongs to superfamily 6 of multicopper oxidases. Read More

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http://dx.doi.org/10.1016/j.nbt.2018.12.001DOI Listing
March 2019
1 Read

Response surface statistical optimization of bacterial nanocellulose fermentation in static culture using a low-cost medium.

N Biotechnol 2019 Mar 6;49:19-27. Epub 2018 Dec 6.

CEB- Centre of Biological Engineering, University of Minho, Campus de Gualtar, 4710-057, Braga, Portugal.

This work aimed at the optimization of bacterial nanocellulose (BNC) production by static culture, using Komagataeibacter xylinus BPR 2001 (K. xylinus). Response surface methodology - central composite design was used to evaluate the effect of inexpensive and widely available nutrient sources, namely molasses, ethanol, corn steep liquor (CSL) and ammonium sulphate, on BNC production yield. Read More

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http://dx.doi.org/10.1016/j.nbt.2018.12.002DOI Listing
March 2019
2 Reads

Oil reservoirs, an exceptional habitat for microorganisms.

N Biotechnol 2019 Mar 28;49:1-9. Epub 2018 Nov 28.

University of Duisburg-Essen, Biofilm Centre, Universitätsstr. 5, 41451, Essen, Germany. Electronic address:

Microorganisms are present in oil reservoirs around the world where they degrade oil and lead to changes in oil quality. Unfortunately, our knowledge about processes in deep oil reservoirs is limited due to the lack of undisturbed samples. In this review, we discuss the distribution of microorganisms at the oil-water transition zone as well as in water saturated parts of the oil leg and their possible physiological adaptations to abiotic and biotic ecological factors such as temperature, salinity and viruses. Read More

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http://dx.doi.org/10.1016/j.nbt.2018.11.006DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6323355PMC

A novel and simple method to produce large amounts of recombinant soluble peptide/major histocompatibility complex monomers for analysis of antigen-specific human T cell receptors.

N Biotechnol 2019 Mar 19;49:169-177. Epub 2018 Nov 19.

Department of Immunology, Graduate School of Medicine and Pharmaceutical Sciences (Medicine), University of Toyama, 2630 Sugitani, Toyama 930-0194, Japan.

Soluble peptide/major histocompatibility complex (p/MHC) tetramers that directly bind to T cell receptors (TCRs) allow the direct quantification, phenotypic characterization and isolation of antigen-specific T cells. Conventionally, soluble p/MHC tetramers have been produced using Escherichia coli, but this method requires refolding of the recombinant proteins. Here, a novel and technically simple method that does not require protein refolding in vitro has been developed for the high-throughput generation of soluble and functional p/MHC-single chain trimer (SCT) monomers and tetramers in a mammalian cell system. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S18716784183167
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http://dx.doi.org/10.1016/j.nbt.2018.11.005DOI Listing
March 2019
11 Reads

Four types of human platelet lysate, including one virally inactivated by solvent-detergent, can be used to propagate Wharton jelly mesenchymal stromal cells.

N Biotechnol 2019 Mar 20;49:151-160. Epub 2018 Nov 20.

Graduate Institute of Biomedical Materials and Tissue Engineering, College of Biomedical Engineering, Taipei Medical University, Taipei, Taiwan; International PhD Program in Biomedical Engineering, College of Biomedical Engineering, Taipei Medical University, Taipei, Taiwan. Electronic address:

There is accumulating experimental evidence that human platelet lysate (HPL) made from platelet concentrates can replace fetal bovine serum (FBS) as a xeno-free clinical-grade supplement of growth media to expand mesenchymal stromal cells (MSCs). However, uncertainties exist in regard to impacts that various manufacturing methods of HPL can exert on the expansion and differentiation capacity of MSCs. In particular, there is a need to evaluate the possibility of implementing virus-inactivation treatment during HPL production to ensure optimal safety of industrial HPL pools. Read More

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http://dx.doi.org/10.1016/j.nbt.2018.11.003DOI Listing
March 2019
21 Reads

Sustainable PHA production in integrated lignocellulose biorefineries.

N Biotechnol 2019 Mar 19;49:161-168. Epub 2018 Nov 19.

Bioresource Engineering Department, McGill University, 21111 Lakeshore Rd., H9X 3V9, Ste-Anne de Bellevue, QC, Canada.

In emerging bioeconomies, the compostable biopolymers polyhydroxyalkanoates (PHAs) are desirable products due to their similarity to petropolymers. While industrial PHA production has been growing rapidly, obtaining a cheap and sustainable carbon source is still a challenge. Among biobased feedstocks, lignocellulose is a cheap, abundant and potentially sustainable carbon source. Read More

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http://dx.doi.org/10.1016/j.nbt.2018.11.004DOI Listing
March 2019
1 Read

The biopolymer produced by Rhizobium viscosum CECT 908 is a promising agent for application in microbial enhanced oil recovery.

N Biotechnol 2019 Mar 13;49:144-150. Epub 2018 Nov 13.

CEB - Centre of Biological Engineering, University of Minho, 4710-057 Braga, Portugal.

Polymer flooding is one of the most promising techniques used to increase the productivity of mature oil reservoirs. Polymers reduce the mobility ratio of the injected water relative to the crude oil, improving the displacement of the entrapped oil and consequently, increasing oil recovery. Biopolymers such as xanthan gum have emerged as environmentally friendly alternatives to the chemical polymers commonly employed by the oil industry. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S18716784183011
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http://dx.doi.org/10.1016/j.nbt.2018.11.002DOI Listing
March 2019
12 Reads

Production of xylanolytic enzymes by Moesziomyces spp. using xylose, xylan and brewery's spent grain as substrates.

N Biotechnol 2019 Mar 10;49:137-143. Epub 2018 Nov 10.

Unit of Bioenergy, National Laboratory of Energy and Geology, Lisbon, Portugal; Department of Chemistry and Bioscience, Section for Sustainable Biotechnology, Aalborg University, Copenhagen, Denmark. Electronic address:

Xylanases play a crucial role in the hydrolysis of xylan-rich hemicelluloses and have wide industrial applications in the fuel, food, feed and pulp and paper industries. The production of these enzymes at low cost is of paramount importance for their commercial deployment. Moesziomyces antarcticus PYCC 5048 and M. Read More

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http://dx.doi.org/10.1016/j.nbt.2018.11.001DOI Listing
March 2019
1 Read

PHA granules help bacterial cells to preserve cell integrity when exposed to sudden osmotic imbalances.

N Biotechnol 2019 Mar 31;49:129-136. Epub 2018 Oct 31.

Faculty of Chemistry, Brno University of Technology, Purkynova 118, 612 00, Brno, Czech Republic. Electronic address:

Polyhydroxyalkanoates (PHA) are microbial polyesters which accumulate as intracellular granules in numerous prokaryotes and mainly serve as storage materials; beyond this primary function, PHA also enhance the robustness of bacteria against various stress factors. We have observed that the presence of PHA in bacterial cells substantially enhances their ability to maintain cell integrity when suddenly exposed to osmotic imbalances. In the case of the non-halophilic bacterium Cupriavidus necator, the presence of PHA decreased plasmolysis-induced cytoplasmic membrane damage during osmotic up-shock, which subsequently enabled the cells to withstand subsequent osmotic downshock. Read More

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http://dx.doi.org/10.1016/j.nbt.2018.10.005DOI Listing
March 2019
1 Read

Effect of amino acids on transcription and translation of key genes in E. coli K and B grown at a steady state in minimal medium.

N Biotechnol 2019 Mar 29;49:120-128. Epub 2018 Oct 29.

Biotechnology Core Laboratory, National Institute of Diabetes and Digestive and Kidney Diseases, National Institute of Health, Bethesda, MD, 20892, United States. Electronic address:

Growing E. coli to high densities is a common strategy for biologicals production. The process is implemented by using complex or minimal media with different feeding strategies. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S18716784183014
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http://dx.doi.org/10.1016/j.nbt.2018.10.004DOI Listing
March 2019
6 Reads
2.900 Impact Factor

The effect of seed sludge on the selection of a photo-EBPR system.

N Biotechnol 2019 Mar 24;49:112-119. Epub 2018 Oct 24.

UCIBIO-REQUIMTE, Department of Chemistry Faculty of Sciences and Technology, Universidade NOVA de Lisboa, 2829-516 Caparica, Portugal.

The phototrophic-enhanced biological phosphorus removal system (photo-EBPR) was recently proposed as an alternative photosynthetic process to conventional phosphorus removal. Previous work showed the possibility of obtaining a photo-EBPR system starting from a culture already enriched in polyphosphate accumulating organisms (PAOs). The present work evaluated whether the same could be achieved starting from conventional activated sludge. Read More

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http://dx.doi.org/10.1016/j.nbt.2018.10.003DOI Listing

Recombinant cell-lysate-catalysed synthesis of uridine-5'-triphosphate from nucleobase and ribose, and without addition of ATP.

N Biotechnol 2019 Mar 19;49:104-111. Epub 2018 Oct 19.

Research School of Chemistry, Australian National University, Canberra, ACT 2601, Australia. Electronic address:

Nucleoside triphosphates (NTPs) are important synthetic targets with diverse applications in therapeutics and diagnostics. Enzymatic routes to NTPs from simple building blocks are attractive, however the cost and complexity of assembling the requisite mixtures of multiple enzymes hinders application. Here, we describe the use of an engineered E. Read More

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http://dx.doi.org/10.1016/j.nbt.2018.10.002DOI Listing

Transnational access to large prospective cohorts in Europe: Current trends and unmet needs.

N Biotechnol 2019 Mar 17;49:98-103. Epub 2018 Oct 17.

Institute for Molecular Medicine Finland FIMM, University of Helsinki, Biomedicum 2U, Tukholmankati 8, 00290, Helsinki, Finland; National Institute for Health and Welfare (THL), Biomedicum 1, Haartmaninkatu 8, 00290, Helsinki, Finland; University of Tartu, Estonian Genome Center, Riia 23b, 51010, Tartu, Estonia.

Biobank samples and data from studies of large prospective cohorts (LPC) represent an invaluable resource for health research. Efficient sharing and pooling of samples and data is a central pre-requisite for new advances in biomedical science. This requirement, however, is not compatible with the present scattered and traditional access governance structures, where legal and ethical frameworks often form an obstacle for effective sharing. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S18716784183177
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http://dx.doi.org/10.1016/j.nbt.2018.10.001DOI Listing
March 2019
21 Reads

Impact of physicochemical properties of DNA/PEI complexes on transient transfection of mammalian cells.

N Biotechnol 2019 Mar 3;49:88-97. Epub 2018 Oct 3.

Department of Chemical Biological and Environmental Engineering, Autonomous University of Barcelona, Bellaterra, Cerdanyola del Vallès, 08193, Barcelona, Spain.

Polyethyleneimine (PEI) has been used extensively for transient gene expression (TGE) in mammalian cell cultures. However, the relationship between DNA/PEI complex preparation and their biological activity has not been fully established. Here, a systematic study of DNA/PEI complexes, their physicochemical properties during formation and their transfection efficiency was performed on a virus-like particle (VLP) production platform. Read More

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http://dx.doi.org/10.1016/j.nbt.2018.09.005DOI Listing

Exploring sialyl-Tn expression in microfluidic-isolated circulating tumour cells: A novel biomarker and an analytical tool for precision oncology applications.

N Biotechnol 2019 Mar 28;49:77-87. Epub 2018 Sep 28.

Portuguese Institute of Oncology, Porto, Portugal; Institute of Biomedical Sciences Abel Salazar, University of Porto, Portugal; International Iberian Nanotechnology Laboratory (INL), Braga, Portugal; Glycobiology in Cancer, Institute of Molecular Pathology and Immunology of the University of Porto (IPATIMUP), Portugal; Instituto de Investigação e Inovação em Saúde (I3S), University of Porto, Portugal. Electronic address:

Circulating tumour cells (CTCs) originating from a primary tumour, lymph nodes and distant metastases hold great potential for liquid biopsies by providing a molecular fingerprint for disease dissemination and its temporal evolution through the course of disease management. CTC enumeration, classically defined on the basis of surface expression of Epithelial Cell Adhesion Molecule (EpCAM) and absence of the pan-leukocyte marker CD45, has been shown to correlate with clinical outcome. However, existing approaches introduce bias into the subsets of captured CTCs, which may exclude biologically and clinically relevant subpopulations. Read More

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http://dx.doi.org/10.1016/j.nbt.2018.09.004DOI Listing
March 2019
22 Reads

Characterization of a novel antioxidant peptide from feather keratin hydrolysates.

N Biotechnol 2019 Mar 14;49:71-76. Epub 2018 Sep 14.

Laboratory of Biochemistry and Applied Microbiology, Federal University of Rio Grande do Sul, 91501-970 Porto Alegre, RS, Brazil. Electronic address:

Feather hydrolysates were obtained through submerged cultivation of 50 g/L feathers with Chryseobacterium sp. kr6. Culture supernatants, displaying antioxidant properties, as evaluated by the 2,2'-azino-bis-(3-ethylbenzothiazoline)-6-sulfonic acid (ABTS) radical scavenging method, were partially purified by gel-filtration chromatography. Read More

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http://dx.doi.org/10.1016/j.nbt.2018.09.003DOI Listing
March 2019
18 Reads

Microbial stress meeting: From systems to molecules and back.

N Biotechnol 2019 Mar 6;49:66-70. Epub 2018 Sep 6.

Bacterial Stress Response Group, Department of Microbiology, National University of Ireland, Galway, Ireland; University of Burgundy, UMR 1347 Agroecoecology, INRA, Dijon, France.

The 4th Microbial Stress Meeting: from Systems to Moleculesand Back was held in April 2018 in Kinsale, Ireland. The meeting covered five main topics: 1. Stress at the systems and structural level; 2. Read More

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http://dx.doi.org/10.1016/j.nbt.2018.09.002DOI Listing
March 2019
7 Reads

Novel insights and innovations in biotechnology towards improved quality of life.

N Biotechnol 2019 Mar 5;49:58-65. Epub 2018 Sep 5.

Institute of Bioorganic Chemistry of the Polish Academy of Sciences, Noskowskiego 12, 61-704 Poznan, Poland. Electronic address:

Resolution of old problems with new tools seems to be a new way of challenging and finding the keys to innovation. A holistic view of different branches of science and industry, including services for society, is critically important for the development of modern science. The International Congress Eurobiotech 2017 was a special opportunity for such a universal view. Read More

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http://dx.doi.org/10.1016/j.nbt.2018.09.001DOI Listing
March 2019
14 Reads

The growing U.S. bioeconomy: Drivers, development and constraints.

N Biotechnol 2019 Mar 16;49:48-57. Epub 2018 Aug 16.

Department of Chemistry, Delaware State University, Dover, DE 19901, USA.

Bioeconomy refers to economic activities using renewable biological resources to produce energy and domestic consumables. The U.S. Read More

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http://dx.doi.org/10.1016/j.nbt.2018.08.005DOI Listing
March 2019
1 Read

Introduction of a process mass intensity metric for biologics.

N Biotechnol 2019 Mar 16;49:37-42. Epub 2018 Aug 16.

Amgen Inc, One Amgen Center Drive, Thousand Oaks, CA 91320, USA.

Biopharmaceuticals (or biologics), large molecule therapeutics typically produced using biotechnology, are a rapidly growing segment of the pharmaceutical market. As such, the environmental footprint of the production of these molecules is coming under scrutiny from various stakeholders such as healthcare providers, investors, and even employees. Process mass intensity (PMI), originally adopted for small molecules by the Green Chemistry Institute Pharmaceutical Roundtable, is a simple metric that can also be applied to evaluate the process efficiency of biopharmaceutical production. Read More

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Bioactive molecules derived from smoke and seaweed Ecklonia maxima showing phytohormone-like activity in Spinacia oleracea L.

N Biotechnol 2019 Jan 9;48:83-89. Epub 2018 Aug 9.

Research Centre for Plant Growth and Development, School of Life Sciences, University of KwaZulu-Natal Pietermaritzburg, Private Bag X01, Scottsville 3209, South Africa. Electronic address:

Bioactive compounds such as karrikinolide (KAR from smoke) and eckol (from the seaweed Ecklonia maxima) show promising effects on several important crop plants. These plant growth-stimulating organic biomolecules, along with crude extracts (smoke-water and Kelpak product prepared from Ecklonia maxima), were tested on spinach plants. Eckol sprayed at 10 M significantly increased all the growth and biochemical parameters examined compared to control spinach plants. Read More

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http://dx.doi.org/10.1016/j.nbt.2018.08.004DOI Listing
January 2019
2 Reads

Is seashell powder suitable for phosphate recovery from fermentation broth?

N Biotechnol 2019 Mar 8;49:43-47. Epub 2018 Aug 8.

Sustainable Chemistry (Resource Efficiency), Institute of Sustainable and Environmental Chemistry, Leuphana University of Lüneburg, Universitätsallee 1, C13.203, 21335 Lüneburg, Germany. Electronic address:

This communication elaborates on the use of seashell powder (SP) for the removal of phosphate from lactic acid-containing fermentation broth. Despite extensive past research regarding the application of SP for phosphate removal from wastewater, no information is available for solutions containing various organic compounds. In order to fill this knowledge gap, tests were performed with pure phosphate solution (PPS) and PPS containing 0. Read More

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Antibody validation: a view from the mountains.

N Biotechnol 2018 Oct 4;45:1-8. Epub 2018 Aug 4.

Department of Neurobiology, Physiology and Behavior, University of California, Davis, CA, 95616, USA; Department of Physiology and Membrane Biology, University of California Davis School of Medicine, Davis, CA, 95616, USA. Electronic address:

Validation of antibodies and other protein binders is a subject of pressing concern for the research community and one which is uppermost in the minds of all who use antibodies as research and diagnostic reagents. Assessing an antibody's fitness for purpose includes accurate ascertainment of its target specificity and suitability for the envisaged task. Moreover, standardised procedures are essential to guarantee sample quality in testing procedures. Read More

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http://dx.doi.org/10.1016/j.nbt.2018.08.002DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6128662PMC
October 2018

New hybrid type strigolactone mimics derived from plant growth regulator auxin.

N Biotechnol 2019 Jan 2;48:76-82. Epub 2018 Aug 2.

Palacký University, Faculty of Science, Centre of the Region Haná for Biotechnological and Agricultural Research, Department of Chemical Biology and Genetics, Šlechtitelů 241/27, CZ-783 71 Olomouc, Czech Republic; Radboud University, Institute for Molecules and Materials, Cluster of Organic Chemistry, Heyendaalsweg 135, 6525AJ Nijmegen, The Netherlands.

Strigolactones (SLs) constitute a new class of plant hormones of increasing importance in plant science. The structure of natural SLs is too complex for ready access by synthesis. Therefore, much attention is being given to design of SL analogues and mimics with a simpler structure but with retention of bioactivity. Read More

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January 2019
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The strong reaction of simple phenolic acids during oxidative stress caused by nickel, cadmium and copper in the microalga Scenedesmus quadricauda.

N Biotechnol 2019 Jan 23;48:66-75. Epub 2018 Jul 23.

Department of Chemistry and Biochemistry, Mendel University in Brno, Zemedelska 1, CZ-613 00 Brno, Czech Republic; Central European Institute of Technology, Brno University of Technology, Purkynova 656/123, 612 00 Brno, Czech Republic. Electronic address:

This work has analysed the influence of CdCl, NiCl and CuCl on simple phenolic acids, such as the 3PPP (phenylpyruvic, phenylacetic, and 4-hydroxyphenylpyruvic) and 2DR (3,4-dihydroxyphenylacetic and rosmarinic) phenolic acids for the first time and studied their interactions with antioxidant systems and the glutathione-ascorbate cycle in the freshwater green microalga Scenedesmus quadricauda. The compounds investigated are related to both the catabolic and anabolic pathways of l-phenylalanine and l-tyrosine, the main molecules in the biosynthesis of polyphenols. The concentrations of the simple phenolic acids responded significantly to NiCl, CdCl and CuCl at 5, 20 and 40 μM concentrations. Read More

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January 2019
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Phytohormones and polyamines regulate plant stress responses by altering GABA pathway.

N Biotechnol 2019 Jan 23;48:53-65. Epub 2018 Jul 23.

Department of Chemical Biology and Genetics, Centre of the Region Haná for Biotechnological and Agricultural Research, Faculty of Science, Palacký University, Olomouc, CZ-78371, Czech Republic. Electronic address:

In plants, γ-aminobutyric acid (GABA) accumulates rapidly in response to environmental stress and variations in its endogenous concentration have been shown to affect plant growth. Exogenous application of GABA has also conferred higher stress tolerance by modulating the expression of genes involved in plant signalling, transcriptional regulation, hormone biosynthesis, reactive oxygen species production and polyamine metabolism. Plant hormones play critical roles in adaptation of plants to adverse environmental conditions through a sophisticated crosstalk among them. Read More

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http://dx.doi.org/10.1016/j.nbt.2018.07.003DOI Listing
January 2019
6 Reads

Industrial potato peel as a feedstock for biobutanol production.

N Biotechnol 2018 Nov 22;46:54-60. Epub 2018 Jul 22.

Biofuels and Bioproducts Research Centre, Institute of Agricultural Technology of Castile and Leon (ITACyL), Villarejo de Órbigo, E-24358 León, Spain; Chemical and Environmental Bioprocess Engineering, Institute of Natural Resources (IRENA), University of Leon, Avenida de Portugal 42, E-24071 León, Spain. Electronic address:

Potato peel from a snack factory was assessed as possible feedstock for biobutanol production. This lignocellulosic biomass was subjected to various physicochemical pretreatments (autohydrolysis and hydrolysis with dilute acids, alkalis, organic solvents or surfactants) under different conditions of time, temperature and reagent concentrations, in order to favour the release of sugars and reduce the generation of fermentation inhibitors. Thereafter, the pretreated potato peel was treated enzymatically to complete the hydrolysis. Read More

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http://dx.doi.org/10.1016/j.nbt.2018.07.002DOI Listing
November 2018
11 Reads

Production of bioactive hepcidin by recombinant DNA tagging with an elastin-like recombinamer.

N Biotechnol 2018 Nov 26;46:45-53. Epub 2018 Jul 26.

CBMA (Centre of Molecular and Environmental Biology), Department of Biology, University of Minho, Campus de Gualtar, 4710-057 Braga, Portugal. Electronic address:

With the lack of new chemical antibiotics and increasing pathogen resistance to those available, new alternatives are being explored. Antimicrobial peptides (AMPs) with a broad range of effects, including antibacterial, antifungal, and antiviral actions, have emerged as one of the options. They can be produced by recombinant DNA technology, but the chromatographic methods used for peptide purification are expensive and time consuming. Read More

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http://dx.doi.org/10.1016/j.nbt.2018.07.001DOI Listing
November 2018
6 Reads

IMTB 2017 Conference: At the intersection of microfluidics and biotechnology.

N Biotechnol 2018 12 28;47:iii-iv. Epub 2018 Jun 28.

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http://dx.doi.org/10.1016/j.nbt.2018.06.005DOI Listing
December 2018
1 Read

Display of the peroxiredoxin Bcp1 of Sulfolobus solfataricus on probiotic spores of Bacillus megaterium.

N Biotechnol 2018 Nov 27;46:38-44. Epub 2018 Jun 27.

Department of Biology, Federico II University of Naples, Italy. Electronic address:

Bacterial spores displaying heterologous proteins have been proposed as a safe and efficient method for delivery of antigens and enzymes to animal mucosal surfaces. Initial studies have been performed using Bacillus subtilis spores, but other spore forming organisms have also been considered. B. Read More

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http://dx.doi.org/10.1016/j.nbt.2018.06.004DOI Listing
November 2018
6 Reads

New fluorescently labeled auxins exhibit promising anti-auxin activity.

N Biotechnol 2019 Jan 25;48:44-52. Epub 2018 Jun 25.

Laboratory of Growth Regulators, Centre of the Region Haná for Biotechnological and Agricultural Research, Faculty of Science of Palacký University & Institute of Experimental Botany of the Czech Academy of Sciences, Šlechtitelů 27, CZ-783 71, Olomouc, Czech Republic; Department of Chemical Biology and Genetics, Centre of the Region Haná for Biotechnological and Agricultural Research, Faculty of Science, Palacký University, Šlechtitelů 27, CZ-783 71, Olomouc, Czech Republic. Electronic address:

The plant hormone auxin is a key player in the regulation of plant growth and development. Despite numerous studies devoted to understanding its role in a wide spectrum of physiological processes, full appreciation of its function is linked to a comprehensive determination of its spatio-temporal distribution, which plays a crucial role in its mode of action. Conjugation of fluorescent tracers to plant hormones enables sensitive and specific visualization of their subcellular and tissue-specific localization and transport in planta, which represents a powerful tool for plant physiology. Read More

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http://dx.doi.org/10.1016/j.nbt.2018.06.003DOI Listing
January 2019
20 Reads

Impact of the treatment of NH emissions from pig farms on greenhouse gas emissions. Quantitative assessment from the literature data.

Authors:
Éric Dumont

N Biotechnol 2018 Nov 20;46:31-37. Epub 2018 Jun 20.

UMR CNRS 6144 GEPEA, IMT Atlantique, Campus de Nantes, La Chantrerie, 4 rue Alfred Kastler, CS 20722, 44307, Nantes Cedex 3, France. Electronic address:

In order to limit ammonia (NH) emissions from pig farms, various air cleaning solutions are widely applied. However, the literature data report that these systems (chemical scrubbers, bioscrubbers and biofilters) can be both inefficient and promote nitrous oxide (NO) production. As air cleaning technologies should not contribute to secondary trace gases that may have a stronger environmental impact than the raw gas compounds themselves, the objective of this study was to quantify the effect of NH treatment in pig farms on greenhouse gas (GHG) emissions. Read More

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http://dx.doi.org/10.1016/j.nbt.2018.06.001DOI Listing
November 2018
3 Reads