3,944 results match your criteria Neuroblastoma Imaging

Ratiometric sensing of Zn with a new benzothiazole-based fluorescent sensor and living cell imaging.

Analyst 2021 Jun 11. Epub 2021 Jun 11.

School of Chemistry and Chemical Engineering, Shanxi University, Taiyuan 030006, PR China.

A new fluorescent probe, 3-(benzo[d]thiazol-2-yl)-5-bromosalicylaldehyde-4N-phenyl thiosemicarbazone (BTT), for ratiometric sensing of Zn2+ ions in methanol/HEPES buffer solution (3 : 2, pH = 7.4) is reported in this paper. The presence of Zn2+ ions yields a significant blue shift in the maximum emission of BTT from 570 nm to 488 nm, accompanied by a clear color change from orange to green. Read More

View Article and Full-Text PDF

Serial Monitoring of Circulating Tumor DNA by Next-Generation Gene Sequencing as a Biomarker of Response and Survival in Patients With Advanced NSCLC Receiving Pembrolizumab-Based Therapy.

JCO Precis Oncol 2021 19;5. Epub 2021 Mar 19.

Division of Hematology/Oncology, Department of Medicine, University of Pennsylvania Perelman School of Medicine, Philadelphia, PA.

Although the majority of patients with metastatic non-small-cell lung cancer (mNSCLC) lacking a detectable targetable mutation will receive pembrolizumab-based therapy in the frontline setting, predicting which patients will experience a durable clinical benefit (DCB) remains challenging.

Materials And Methods: Patients with mNSCLC receiving pembrolizumab monotherapy or in combination with chemotherapy underwent a 74-gene next-generation sequencing panel on blood samples obtained at baseline and at 9 weeks. The change in circulating tumor DNA levels on-therapy (molecular response) was quantified using a ratio calculation with response defined by a > 50% decrease in mean variant allele fraction. Read More

View Article and Full-Text PDF

Horner Syndrome Secondary to Osteochondroma of the First Rib: A Case Report.

Cureus 2021 Apr 17;13(4):e14531. Epub 2021 Apr 17.

Internal Medicine, Detroit Medical Center (DMC), Detroit, USA.

Osteochondroma is the most common benign tumor of bone that often produces no symptoms unless the enlarged mass affects nearby structures. Rarely, Horner syndrome can be caused by an osteochondroma. A five-year-old female with a past medical history of seizure-like activity presented to the emergency department on three separate occasions within one month. Read More

View Article and Full-Text PDF

Cell surface Nucleolin represents a novel cellular target for neuroblastoma therapy.

J Exp Clin Cancer Res 2021 Jun 2;40(1):180. Epub 2021 Jun 2.

Laboratory of Experimental Therapies in Oncology, IRCCS Istituto Giannina Gaslini, Genoa, Italy.

Background: Neuroblastoma (NB) represents the most frequent and aggressive form of extracranial solid tumor of infants. Nucleolin (NCL) is a protein overexpressed and partially localized on the cell surface of tumor cells of adult cancers. Little is known about NCL and pediatric tumors and nothing is reported about cell surface NCL and NB. Read More

View Article and Full-Text PDF

Theranostics in Neuroblastoma.

PET Clin 2021 Jul;16(3):419-427

Division of Haematology/Oncology, Department of Paediatrics, The Hospital for Sick Children, University of Toronto, 555 University Ave, Toronto, ON M5G 1X8, Canada.

Theranostics combines diagnosis and targeted therapy, achieved by the use of the same or similar molecules labeled with different radiopharmaceuticals or identical with different dosages. One of the best examples is the use of metaiodobenzylguanidine (MIBG). In the management of neuroblastoma-the most common extracranial solid tumor in children. Read More

View Article and Full-Text PDF

Synthesis and evaluation of multivalent nitroimidazole-based near-infrared fluorescent agents for neuroblastoma and colon cancer imaging.

Bioorg Chem 2021 May 18;113:104990. Epub 2021 May 18.

Department of Nuclear Medicine, Molecular Imaging & Therapeutic Medicine Research Center, Jeonbuk National University Medical School and Hospital, Jeonju 54907, Republic of Korea; Research Institute of Clinical Medicine of Jeonbuk National University-Biomedical Research Institute of Jeonbuk National University Hospital, Jeonju 54907, Republic of Korea. Electronic address:

Hypoxia is one of key characteristics of microenvironments of solid tumors, and evaluation of hypoxia status in solid tumors is important to determine cancer stage and appropriate treatment. In the present study, novel, multivalent, near-infrared (NIR) fluorescent imaging agents were developed to measure tumor hypoxia. These agents were synthesized using an amino acid as a backbone to connect mono-, bis-, or tris-2-nitroimidazole as a hypoxia-sensitive moiety to enhance uptake by the tumor and to attach sulfo-Cyanine 5. Read More

View Article and Full-Text PDF

Pinhole Closure Improves Spatial Resolution in Confocal Scanning Microscopy.

Akira Kitamura

Methods Mol Biol 2021 ;2274:385-389

Laboratory of Molecular Cell Dynamics, Faculty of Advanced Life Science, Hokkaido University, Sapporo, Japan.

Confocal microscopy is a simple, super-resolution technique, which does not produce a marked increase in resolution compared to other advanced techniques, such as super-resolution nanoscopy. Here, we present a simple protocol to acquire "slightly, but easily resolved" images by pinhole closure (<1 airy unit) in a conventional confocal scanning microscope equipped with an avalanche photodiode, a detector with high sensitivity. We use murine neuroblastoma Neuro2a cells to demonstrate the image resolution obtained via this protocol without the use of any special software to enhance image quality. Read More

View Article and Full-Text PDF
January 2021

Iodine-GD2-ch14.18 scintigraphy to evaluate option for radioimmunotherapy in patients with advanced tumors.

J Nucl Med 2021 May 28. Epub 2021 May 28.

Department of Nuclear Medicine and Clinical Molecular Imaging, University Hospital Tuebingen, Germany.

The tumor-selective ganglioside antigene GD2 is frequently expressed on neuroblastomas and to a lesser extent also on sarcomas and neuroendocrine tumors. Aim of our study was to evaluate tumor targeting and biodistribution of iodine-131-labeled chimeric GD2-antibody clone 14/18 (I-GD2-ch14.18) in patients with late-stage disease in order to identify eligibility for radioimmunotherapy. Read More

View Article and Full-Text PDF

Inhibition of glycolysis and mitochondrial respiration promotes radiosensitisation of neuroblastoma and glioma cells.

Cancer Metab 2021 May 19;9(1):24. Epub 2021 May 19.

Institute of Cancer Sciences, University of Glasgow, Glasgow, G61 1QH, UK.

Background: Neuroblastoma accounts for 7% of paediatric malignancies but is responsible for 15% of all childhood cancer deaths. Despite rigorous treatment involving chemotherapy, surgery, radiotherapy and immunotherapy, the 5-year overall survival rate of high-risk disease remains < 40%, highlighting the need for improved therapy. Since neuroblastoma cells exhibit aberrant metabolism, we determined whether their sensitivity to radiotherapy could be enhanced by drugs affecting cancer cell metabolism. Read More

View Article and Full-Text PDF

A Novel pH-Sensitive Multifunctional DNA Nanomedicine: An Enhanced and Harmless GD2 Aptamer-Mediated Strategy for Guiding Neuroblastoma Antitumor Therapy.

Int J Nanomedicine 2021 10;16:3217-3240. Epub 2021 May 10.

Department of Neonatology, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi, People's Republic of China.

Background: GD2 is a mainstream biomarker for neuroblastoma (NB)-targeted therapy. Current anti-GD2 therapeutics exhibit several side effects since GD2 is also expressed at low levels on normal cells. Thus, current anti-GD2 therapeutics can be compromised by the coexistence of the target receptor on both cancer cells and normal cells. Read More

View Article and Full-Text PDF

Radiogenomics prediction for MYCN amplification in neuroblastoma: A hypothesis generating study.

Pediatr Blood Cancer 2021 May 18:e29110. Epub 2021 May 18.

UOC Radioterapia Oncologica, Dipartimento di Diagnostica per Immagini, Radioterapia Oncologica ed Ematologia, Fondazione Policlinico Universitario "A. Gemelli" IRCCS, Rome, Italy.

Background: MYCN amplification represents a powerful prognostic factor in neuroblastoma (NB) and may occasionally account for intratumoral heterogeneity. Radiomics is an emerging field of advanced image analysis that aims to extract a large number of quantitative features from standard radiological images, providing valuable clinical information.

Procedure: In this retrospective study, we aimed to create a radiogenomics model by correlating computed tomography (CT) radiomics analysis with MYCN status. Read More

View Article and Full-Text PDF

On the mechanism of tumor cell entry of aloe-emodin, a natural compound endowed with anticancer activity.

Int J Cancer 2021 May 14. Epub 2021 May 14.

Department of Molecular Medicine, University of Padova, Padova, Italy.

Aloe-emodin (1,8-dihydroxy-3-[hydroxymethyl]-anthraquinone), AE, is one of the active constituents of a number of plant species used in traditional medicine. We have previously identified, for the first time, AE as a new antitumor agent and shown that its selective in vitro and in vivo killing of neuroblastoma cells was promoted by a cell-specific drug uptake process. However, the molecular mechanism underlying the cell entry of AE has remained elusive as yet. Read More

View Article and Full-Text PDF

Hutchison's syndrome: suspecting metastatic neuroblastoma.

BMJ Case Rep 2021 May 13;14(5). Epub 2021 May 13.

Department of Ophthalmic Plastics, Orbit and Ocular Oncology, LV Prasad Eye Institute Bhubaneswar Campus, Bhubaneswar, Odisha, India

View Article and Full-Text PDF

DICOM-MIABIS integration model for biobanks: a use case of the EU PRIMAGE project.

Eur Radiol Exp 2021 May 12;5(1):20. Epub 2021 May 12.

Department of Translational Research on New Technologies in Medicine and Surgery, University of Pisa, Pisa, Italy.

PRIMAGE is a European Commission-financed project dealing with medical imaging and artificial intelligence aiming to create an imaging biobank in oncology. The project includes a task dedicated to the interoperability between imaging and standard biobanks. We aim at linking Digital imaging and Communications in Medicine (DICOM) metadata to the Minimum Information About BIobank data Sharing (MIABIS) standard of biobanking. Read More

View Article and Full-Text PDF

Sulfopin is a covalent inhibitor of Pin1 that blocks Myc-driven tumors in vivo.

Nat Chem Biol 2021 May 10. Epub 2021 May 10.

Department of Cancer Biology, Dana-Farber Cancer Institute, Boston, MA, USA.

The peptidyl-prolyl isomerase, Pin1, is exploited in cancer to activate oncogenes and inactivate tumor suppressors. However, despite considerable efforts, Pin1 has remained an elusive drug target. Here, we screened an electrophilic fragment library to identify covalent inhibitors targeting Pin1's active site Cys113, leading to the development of Sulfopin, a nanomolar Pin1 inhibitor. Read More

View Article and Full-Text PDF

SSTR2 Expression in Olfactory Neuroblastoma: Clinical and Therapeutic Implications.

Head Neck Pathol 2021 Apr 30. Epub 2021 Apr 30.

Department of Pathology and Laboratory Medicine, University of Pittsburgh Medical Center, Pittsburgh, PA, USA.

Somatostatin receptor 2 (SSTR2) expression has previously been documented in olfactory neuroblastoma (ONB). Here, we fully characterize SSTR2 expression in ONB and correlate staining results with clinicopathologic parameters including Hyams grade. We also assess SSTR2 immunohistochemistry expression in various histologic mimics of ONB to assess its diagnostic functionality. Read More

View Article and Full-Text PDF

Immune Monitoring during Therapy Reveals Activitory and Regulatory Immune Responses in High-Risk Neuroblastoma.

Cancers (Basel) 2021 Apr 26;13(9). Epub 2021 Apr 26.

Princess Máxima Center for Pediatric Oncology, Utrecht University, 3584 CS Utrecht, The Netherlands.

Despite intensive treatment, including consolidation immunotherapy (IT), prognosis of high-risk neuroblastoma (HR-NBL) is poor. Immune status of patients over the course of treatment, and thus immunological features potentially explaining therapy efficacy, are largely unknown. In this study, the dynamics of immune cell subsets and their function were explored in 25 HR-NBL patients at diagnosis, during induction chemotherapy, before high-dose chemotherapy, and during IT. Read More

View Article and Full-Text PDF

Bergamottin and 5-Geranyloxy-7-methoxycoumarin Cooperate in the Cytotoxic Effect of (Bergamot) Essential Oil in Human Neuroblastoma SH-SY5Y Cell Line.

Toxins (Basel) 2021 04 10;13(4). Epub 2021 Apr 10.

Department of Chemical, Biological, Pharmaceutical and Environmental Sciences, University of Messina, 98168 Messina, Italy.

The plant kingdom has always been a treasure trove for valuable bioactive compounds, and fruits stand out among the others. Bergamottin (BRG) and 5-geranyloxy-7-methoxycoumarin (5-G-7-MOC) are two coumarins found in different species with well-acknowledged pharmacological properties. Previously, they have been claimed to be relevant in the anti-proliferative effects exerted by bergamot essential oil (BEO) in the SH-SY5Y human neuroblastoma cells. Read More

View Article and Full-Text PDF

Metastatic Neuroblastoma Presenting as a Submandibular Mass with Mandibular Bone Involvement in a Three-Year-Old Child.

Int J Environ Res Public Health 2021 04 14;18(8). Epub 2021 Apr 14.

Department of Pediatric Surgery, Medical College of Georgia at Augusta University, Augusta, GA 30912, USA.

Although neuroblastoma is one of the most common extra-cranial tumors in the pediatric population, it is rarely seen as a metastasis to the mandibular bone. The following is a case report of a 3-year-old male who initially presented with a submandibular mass that was proven to be a poorly differentiated metastatic neuroblastoma through excisional biopsy. This report is one of the few case reports that demonstrates metastatic submandibular neuroblastoma with mandibular bone involvement in the pediatric population. Read More

View Article and Full-Text PDF

Nuclear Medicine Imaging in Neuroblastoma: Current Status and New Developments.

J Pers Med 2021 Apr 4;11(4). Epub 2021 Apr 4.

Princess Maxima Center for Pediatric Oncology, Heidelberglaan 25, 3584 CS Utrecht, The Netherlands.

Neuroblastoma is the most common extracranial solid malignancy in children. At diagnosis, approximately 50% of patients present with metastatic disease. These patients are at high risk for refractory or recurrent disease, which conveys a very poor prognosis. Read More

View Article and Full-Text PDF

Heterogeneities in Cell Cycle Checkpoint Activation Following Doxorubicin Treatment Reveal Targetable Vulnerabilities in Mutated Ultra High-Risk Neuroblastoma Cell Lines.

Int J Mol Sci 2021 Apr 1;22(7). Epub 2021 Apr 1.

Department of Women's and Children's Health, Karolinska Institutet, 171 64 Stockholm, Sweden.

Most chemotherapeutics target DNA integrity and thereby trigger tumour cell death through activation of DNA damage responses that are tightly coupled to the cell cycle. Disturbances in cell cycle regulation can therefore lead to treatment resistance. Here, a comprehensive analysis of cell cycle checkpoint activation following doxorubicin (doxo) treatment was performed using flow cytometry, immunofluorescence and live-cell imaging in a panel of mutated ultra high-risk neuroblastoma (NB) cell lines, SK-N-DZ, Kelly, SK-N-AS, SK-N-FI, and BE(2)-C. Read More

View Article and Full-Text PDF

A new approach to analysis of intracellular proteins and subcellular localization using cellprofiler and imageJ in combination.

Methods 2021 Apr 26. Epub 2021 Apr 26.

Department of Physiology, Keio University School of Medicine, Tokyo, Japan. Electronic address:

Analytical pipeline, which is used for various analysis application, of CellProfiler, an open-source software for cell imaging analysis, is very important. In the present study, to examine whether intracellular proteins can be discriminated using a combination of CellProfiler and ImageJ, we analyzed neuroblastoma and monocytic cell lines, and disease-specific induced pluripotent stem cell (iPSC)-derived neurons. This revealed that scattered puncta of Rab7 and transferrin in neuroblastoma lines were clearly detectable by created analytical pipelines in CellProfiler. Read More

View Article and Full-Text PDF

Neuroblastoma metastasized to the mandible and the spinal extradural regions: A case report.

Medicine (Baltimore) 2021 Apr;100(17):e24399

Cancer Center.

Abstract: Neuroblastoma (NB) metastasized into the mandible and spinal extradural region was rarely reported. We present a case with metastatic NB to the mandible and the spinal extradural regions. The patient received chemotherapy using NB 97 regimen and was tumor free after 8 months, but 9 months after the treatment, the patient presented with lower limb paralysis and persistent pain in maxillofacial region as well as swelling in the left mandibular area. Read More

View Article and Full-Text PDF

Selective modulation of a pan-essential protein as a therapeutic strategy in cancer.

Cancer Discov 2021 Apr 21. Epub 2021 Apr 21.

Department of Pediatric Oncology, Dana-Farber Cancer Institute

Cancer dependency maps, which use CRISPR/Cas9 depletion screens to profile the landscape of genetic dependencies in hundreds of cancer cell lines, have identified context-specific dependencies that could be therapeutically exploited. An ideal therapy is both lethal and precise, but these depletion screens cannot readily distinguish between gene effects that are cytostatic or cytotoxic. Here, we employ a diverse panel of functional genomic screening assays to identify NXT1 as a selective and rapidly lethal in vivo-relevant genetic dependency in MYCN-amplified neuroblastoma. Read More

View Article and Full-Text PDF

Effect of ethanol and cocaine on [C]MPC-6827 uptake in SH-SY5Y cells.

Mol Biol Rep 2021 Apr 20;48(4):3871-3876. Epub 2021 Apr 20.

Department of Radiology, Wake Forest School of Medicine, Winston-Salem, NC, 27157, USA.

Microtubules (MTs) are structural units in the cytoskeleton. In brain cells they are responsible for axonal transport, information processing, and signaling mechanisms. Proper function of these processes is critical for healthy brain functions. Read More

View Article and Full-Text PDF

A single quantum dot-based fluorescence resonance energy transfer biosensor for antibody-free detection of ten-eleven translocation 1.

Chem Commun (Camb) 2021 Apr 26;57(29):3543-3546. Epub 2021 Mar 26.

College of Chemistry, Chemical Engineering and Materials Science, Collaborative Innovation Center of Functionalized Probes for Chemical Imaging in Universities of Shandong, Key Laboratory of Molecular and Nano Probes, Ministry of Education, Shandong Provincial Key Laboratory of Clean Production of Fine Chemicals, Shandong Normal University, Jinan 250014, China.

We developed a single quantum dot-based fluorescence resonance energy transfer biosensor for antibody-free detection of ten-eleven translocation 1 (TET1). This biosensor can sensitively detect TET1 in a homogeneous manner without the involvement of any specific antibodies, and it can be used for accurate measurement of TET1 activity in human neuroblastoma cells and the screening of TET1 inhibitors. Read More

View Article and Full-Text PDF

Comparison of the detecting capability between I-mIBG and post-therapeutic I-mIBG scintigraphy for curie scoring in patients with neuroblastoma after chemotherapy.

Ann Nucl Med 2021 Jun 17;35(6):649-661. Epub 2021 Apr 17.

Department of Nuclear Medicine, Kanazawa University Hospital, 13-1 Takara-machi, Kanazawa, Ishikawa, 920-8641, Japan.

Objective: To evaluate the detecting capability between planar imaging (PI) and PI combined with single-photon emission computed tomography/computed tomography (PICWS), including I- and I-labeled metaiodobenzylguanidine (mIBG) and to compare the detecting capability between I-mIBG and post-therapeutic I-mIBG scintigraphy including PI and PICWS for Curie scoring in patients with neuroblastoma.

Methods: Sixty-two patients with 66 pairs of complete images with neuroblastoma were enrolled in this retrospective study.

Results: Comparing the Curie scoring between I-mIBG PI and PICWS and between post-therapeutic I-mIBG PI and PICWS, findings were concordantly negative in 28. Read More

View Article and Full-Text PDF

Epigallocatechin-3-gallate inhibits the growth of three-dimensional in vitro models of neuroblastoma cell SH-SY5Y.

Mol Cell Biochem 2021 Apr 16. Epub 2021 Apr 16.

Target Discovery Institute, Nuffield Department of Medicine, University of Oxford, Oxford, England, UK.

The aim of the study is to investigate the potential of using three-dimensional (3D) in vitro neuroblastoma models to mimic the neuroblastoma microenvironment by testing a potential therapeutic compound-the natural extract epigallocatechin-3-gallate (EGCG), and to further elucidate the roles of DYRK1A in the growth and differentiation of neuroblastoma tissue. In vitro models based on a classic neuroblastoma cell line SH-SY5Y were employed, including 3D models with extracellular matrix and co-cultured with vascular endothelial cells. Cell viability was tested using AlamarBlue and Resazurin assay. Read More

View Article and Full-Text PDF

Novel Peptide-Calix[4]arene Conjugate Inhibits Aβ Aggregation and Rescues Neurons from Aβ's Oligomers Cytotoxicity .

ACS Chem Neurosci 2021 04 12;12(8):1449-1462. Epub 2021 Apr 12.

CNR-Institute of Crystallography, Via P. Gaifami 18, 95126 Catania, Italy.

Alzheimer's disease (AD) is a progressive neurodegenerative condition affecting people in the elderly. Targeting aggregation of β-amyloid peptides (Aβ) is considered a promising approach for the therapeutic treatment of the disease. Peptide based inhibitors of β-amyloid fibrillation are emerging as safe drug candidates as well as interesting compounds for early diagnosis of AD. Read More

View Article and Full-Text PDF

IRE1α-XBP1 Affects the Mitochondrial Function of Aβ25-35-Treated SH-SY5Y Cells by Regulating Mitochondria-Associated Endoplasmic Reticulum Membranes.

Front Cell Neurosci 2021 25;15:614556. Epub 2021 Mar 25.

Department of Neurology, Second Hospital of Shandong University, Jinan, China.

Neurotoxicity induced by the amyloid beta (Aβ) peptide is one of the most important pathological mechanisms of Alzheimer's disease (AD). Activation of the adaptive IRE1α-XBP1 pathway contributes to the pathogenesis of AD, making it a potential target for AD therapeutics. However, the mechanism of IRE1α-XBP1 pathway involvement in AD is unclear. Read More

View Article and Full-Text PDF