9,383 results match your criteria Nature Biotechnology[Journal]


Protection of tissue physicochemical properties using polyfunctional crosslinkers.

Nat Biotechnol 2018 Dec 17. Epub 2018 Dec 17.

Institute for Medical Engineering and Science, Massachusetts Institute of Technology (MIT), Cambridge, Massachusetts, USA.

Understanding complex biological systems requires the system-wide characterization of both molecular and cellular features. Existing methods for spatial mapping of biomolecules in intact tissues suffer from information loss caused by degradation and tissue damage. We report a tissue transformation strategy named stabilization under harsh conditions via intramolecular epoxide linkages to prevent degradation (SHIELD), which uses a flexible polyepoxide to form controlled intra- and intermolecular cross-link with biomolecules. Read More

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http://www.nature.com/doifinder/10.1038/nbt.4281
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http://dx.doi.org/10.1038/nbt.4281DOI Listing
December 2018
2 Reads

Minimum Information about an Uncultivated Virus Genome (MIUViG).

Nat Biotechnol 2018 Dec 17. Epub 2018 Dec 17.

US Department of Energy Joint Genome Institute, Walnut Creek, California, USA.

We present an extension of the Minimum Information about any (x) Sequence (MIxS) standard for reporting sequences of uncultivated virus genomes. Minimum Information about an Uncultivated Virus Genome (MIUViG) standards were developed within the Genomic Standards Consortium framework and include virus origin, genome quality, genome annotation, taxonomic classification, biogeographic distribution and in silico host prediction. Community-wide adoption of MIUViG standards, which complement the Minimum Information about a Single Amplified Genome (MISAG) and Metagenome-Assembled Genome (MIMAG) standards for uncultivated bacteria and archaea, will improve the reporting of uncultivated virus genomes in public databases. Read More

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http://www.nature.com/doifinder/10.1038/nbt.4306
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http://dx.doi.org/10.1038/nbt.4306DOI Listing
December 2018
1 Read

Deep learning using tumor HLA peptide mass spectrometry datasets improves neoantigen identification.

Nat Biotechnol 2018 Dec 17. Epub 2018 Dec 17.

Gritstone Oncology, Inc., Emeryville, California and Cambridge, Massachusetts, USA.

Neoantigens, which are expressed on tumor cells, are one of the main targets of an effective antitumor T-cell response. Cancer immunotherapies to target neoantigens are of growing interest and are in early human trials, but methods to identify neoantigens either require invasive or difficult-to-obtain clinical specimens, require the screening of hundreds to thousands of synthetic peptides or tandem minigenes, or are only relevant to specific human leukocyte antigen (HLA) alleles. We apply deep learning to a large (N = 74 patients) HLA peptide and genomic dataset from various human tumors to create a computational model of antigen presentation for neoantigen prediction. Read More

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http://dx.doi.org/10.1038/nbt.4313DOI Listing
December 2018

Dimensionality reduction for visualizing single-cell data using UMAP.

Nat Biotechnol 2018 Dec 3. Epub 2018 Dec 3.

Singapore Immunology Network (SIgN), Agency for Science, Technology and Research (A*STAR), Singapore, Singapore.

Advances in single-cell technologies have enabled high-resolution dissection of tissue composition. Several tools for dimensionality reduction are available to analyze the large number of parameters generated in single-cell studies. Recently, a nonlinear dimensionality-reduction technique, uniform manifold approximation and projection (UMAP), was developed for the analysis of any type of high-dimensional data. Read More

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http://dx.doi.org/10.1038/nbt.4314DOI Listing
December 2018

Real-time measurement of protein-protein interactions at single-molecule resolution using a biological nanopore.

Nat Biotechnol 2018 Dec 10. Epub 2018 Dec 10.

Department of Physics, Syracuse University, Syracuse, New York, USA.

Protein-protein interactions (PPIs) are essential for many cellular processes. However, transient PPIs are difficult to measure at high throughput or in complex biological fluids using existing methods. We engineered a genetically encoded sensor for real-time sampling of transient PPIs at single-molecule resolution. Read More

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http://www.nature.com/doifinder/10.1038/nbt.4316
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http://dx.doi.org/10.1038/nbt.4316DOI Listing
December 2018
5 Reads

To hear a whisper: biotechs chase new thinking to restore hearing.

Authors:
Dan Jones

Nat Biotechnol 2018 Dec;36(12):1128-1129

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http://dx.doi.org/10.1038/nbt1218-1128DOI Listing
December 2018

The European Open Science Cloud and commercialization.

Authors:
Helen Yu

Nat Biotechnol 2018 Dec;36(12):1133-1134

Faculty of Law, University of Copenhagen, Copenhagen, Denmark.

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http://dx.doi.org/10.1038/nbt.4304DOI Listing
December 2018
1 Read

Radioactive drugs emerge from the shadows to storm the market.

Authors:
Elie Dolgin

Nat Biotechnol 2018 Dec;36(12):1125-1127

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http://dx.doi.org/10.1038/nbt1218-1125DOI Listing
December 2018

Research Highlights.

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Nat Biotechnol 2018 Dec;36(12):1154

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http://dx.doi.org/10.1038/nbt.4307DOI Listing
December 2018

A reference standard for genome biology.

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Nat Biotechnol 2018 Dec;36(12):1121

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http://dx.doi.org/10.1038/nbt.4318DOI Listing
December 2018

First edible cottonseed go-ahead.

Authors:
Emily Waltz

Nat Biotechnol 2018 Dec;36(12):1126

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http://dx.doi.org/10.1038/nbt1218-1126DOI Listing
December 2018

Biopharmaceutical benchmarks 2018.

Authors:
Gary Walsh

Nat Biotechnol 2018 Dec;36(12):1136-1145

Industrial Biochemistry Program, Department of Chemical Sciences and Bernal Institute, University of Limerick, Ireland.

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http://dx.doi.org/10.1038/nbt.4305DOI Listing
December 2018

People.

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Nat Biotechnol 2018 Dec;36(12):1220

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http://dx.doi.org/10.1038/nbt.4321DOI Listing
December 2018

Drug pipeline 3Q18.

Nat Biotechnol 2018 Dec;36(12):1132

Nature Biotechnology.

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http://www.nature.com/doifinder/10.1038/nbt.4310
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http://dx.doi.org/10.1038/nbt.4310DOI Listing
December 2018
3 Reads

Was the Myriad decision a 'surgical strike' on isolated DNA patents, or does it have wider impacts?

Nat Biotechnol 2018 Dec;36(12):1146-1149

Centre for Law, Medicine, and Life Sciences, Faculty of Law, University of Cambridge, Cambridge, UK.

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http://dx.doi.org/10.1038/nbt.4308DOI Listing
December 2018

Mouse development hits the big screen.

Authors:
Markus Elsner

Nat Biotechnol 2018 Dec;36(12):1155

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http://dx.doi.org/10.1038/nbt.4315DOI Listing
December 2018

Correction.

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Nat Biotechnol 2018 Dec;36(12):1131

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http://dx.doi.org/10.1038/nbt1218-1131bDOI Listing
December 2018

The myriad targets of a T cell.

Nat Biotechnol 2018 Dec;36(12):1152-1154

Department of Pathology and Perlmutter Cancer Center, New York University School of Medicine, New York, New York, USA.

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http://dx.doi.org/10.1038/nbt.4309DOI Listing
December 2018

T cells fingered as culprits for narcolepsy.

Authors:
Barbara Nasto

Nat Biotechnol 2018 Dec;36(12):1130

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http://dx.doi.org/10.1038/nbt1218-1130DOI Listing
December 2018

Around the world in a month.

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Nat Biotechnol 2018 Dec;36(12):1131

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http://dx.doi.org/10.1038/nbt1218-1131aDOI Listing
December 2018

RNAi biotechs flush with pharma dollars.

Authors:
Joana Osorio

Nat Biotechnol 2018 Dec;36(12):1127

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http://dx.doi.org/10.1038/nbt1218-1127DOI Listing
December 2018

Building a career planning course for STEM PhDs.

Nat Biotechnol 2018 Dec;36(12):1217-1219

Career and Postdoctoral Services Office, The Scripps Research Institute, La Jolla, California, USA.

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http://dx.doi.org/10.1038/nbt.4312DOI Listing
December 2018

Roche's cell squeeze provokes killers.

Authors:
Joana Osorio

Nat Biotechnol 2018 Dec;36(12):1129

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http://dx.doi.org/10.1038/nbt1218-1129DOI Listing
December 2018

Recent patents in neuroengineering.

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Nat Biotechnol 2018 Dec;36(12):1150

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http://dx.doi.org/10.1038/nbt.4320DOI Listing
December 2018

RNA epigenetics spurs investor interest, but uncertainties linger.

Authors:
John Hodgson

Nat Biotechnol 2018 Dec;36(12):1123-1124

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http://dx.doi.org/10.1038/nbt1218-1123DOI Listing
December 2018

Taming preeclampsia at its source.

Nat Biotechnol 2018 Dec;36(12):1151-1152

Nuffield Department of Women's & Reproductive Health, University of Oxford, Oxford, UK.

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http://dx.doi.org/10.1038/nbt.4311DOI Listing
December 2018
1 Read

Predicting the mutations generated by repair of Cas9-induced double-strand breaks.

Nat Biotechnol 2018 Nov 27. Epub 2018 Nov 27.

Wellcome Sanger Institute, Hinxton, UK.

The DNA mutation produced by cellular repair of a CRISPR-Cas9-generated double-strand break determines its phenotypic effect. It is known that the mutational outcomes are not random, but depend on DNA sequence at the targeted location. Here we systematically study the influence of flanking DNA sequence on repair outcome by measuring the edits generated by >40,000 guide RNAs (gRNAs) in synthetic constructs. Read More

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http://www.nature.com/doifinder/10.1038/nbt.4317
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November 2018
12 Reads

An oncolytic herpesvirus expressing E-cadherin improves survival in mouse models of glioblastoma.

Nat Biotechnol 2018 Nov 26. Epub 2018 Nov 26.

Division of Hematology, Department of Internal Medicine, College of Medicine, The Ohio State University, Columbus, Ohio, USA.

The efficacy of oncolytic herpes simplex virus (oHSV) is limited by rapid viral clearance by innate immune effector cells and poor intratumoral viral spread. We combine two approaches to overcome these barriers: inhibition of natural killer (NK) cells and enhancement of intratumoral viral spread. We engineered an oHSV to express CDH1, encoding E-cadherin, an adherent molecule and a ligand for KLRG1, an inhibitory receptor expressed on NK cells. Read More

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http://dx.doi.org/10.1038/nbt.4302DOI Listing
November 2018
1 Read

T cell receptor fingerprinting enables in-depth characterization of the interactions governing recognition of peptide-MHC complexes.

Nat Biotechnol 2018 Nov 19. Epub 2018 Nov 19.

Department of Micro and Nanotechnology, Technical University of Denmark, Lyngby, Denmark.

The promiscuous nature of T-cell receptors (TCRs) allows T cells to recognize a large variety of pathogens, but makes it challenging to understand and control T-cell recognition. Existing technologies provide limited information about the key requirements for T-cell recognition and the ability of TCRs to cross-recognize structurally related elements. Here we present a 'one-pot' strategy for determining the interactions that govern TCR recognition of peptide-major histocompatibility complex (pMHC). Read More

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http://dx.doi.org/10.1038/nbt.4303DOI Listing
November 2018
19 Reads

A massively parallel reporter assay dissects the influence of chromatin structure on cis-regulatory activity.

Nat Biotechnol 2018 Nov 19. Epub 2018 Nov 19.

The Edison Family Center for Genome Sciences and Systems Biology, Washington University School of Medicine, Saint Louis, Missouri, USA.

A gene's position in the genome can profoundly affect its expression because regional differences in chromatin modulate the activity of locally acting cis-regulatory sequences (CRSs). Here we study how CRSs and regional chromatin act in concert on a genome-wide scale. We present a massively parallel reporter gene assay that measures the activities of hundreds of different CRSs, each integrated at many specific genomic locations. Read More

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http://www.nature.com/doifinder/10.1038/nbt.4285
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http://dx.doi.org/10.1038/nbt.4285DOI Listing
November 2018
4 Reads

RNAi modulation of placental sFLT1 for the treatment of preeclampsia.

Nat Biotechnol 2018 Nov 19. Epub 2018 Nov 19.

RNA Therapeutics Institute, University of Massachusetts Medical School, Worcester, Massachusetts, USA.

Preeclampsia is a placentally induced hypertensive disorder of pregnancy that is associated with substantial morbidity and mortality to mothers and fetuses. Clinical manifestations of preterm preeclampsia result from excess circulating soluble vascular endothelial growth factor receptor FLT1 (sFLT1 or sVEGFR1) of placental origin. Here we identify short interfering RNAs (siRNAs) that selectively silence the three sFLT1 mRNA isoforms primarily responsible for placental overexpression of sFLT1 without reducing levels of full-length FLT1 mRNA. Read More

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http://www.nature.com/doifinder/10.1038/nbt.4297
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http://dx.doi.org/10.1038/nbt.4297DOI Listing
November 2018
23 Reads
41.514 Impact Factor

High-throughput determination of the antigen specificities of T cell receptors in single cells.

Nat Biotechnol 2018 Nov 12. Epub 2018 Nov 12.

Institute for Cellular and Molecular Biology, University of Texas at Austin, Austin, Texas, USA.

We present tetramer-associated T-cell receptor sequencing (TetTCR-seq) to link T cell receptor (TCR) sequences to their cognate antigens in single cells at high throughput. Binding is determined using a library of DNA-barcoded antigen tetramers that is rapidly generated by in vitro transcription and translation. We applied TetTCR-seq to identify patterns in TCR cross-reactivity with cancer neoantigens and to rapidly isolate neoantigen-specific TCRs with no cross-reactivity to the wild-type antigen. Read More

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https://www.nature.com/articles/nbt.4282.pdf
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http://www.nature.com/doifinder/10.1038/nbt.4282
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http://dx.doi.org/10.1038/nbt.4282DOI Listing
November 2018
27 Reads

ClampFISH detects individual nucleic acid molecules using click chemistry-based amplification.

Nat Biotechnol 2018 Nov 12. Epub 2018 Nov 12.

Department of Bioengineering, University of Pennsylvania, Philadelphia Pennsylvania, USA.

Methods for detecting single nucleic acids in cell and tissues, such as fluorescence in situ hybridization (FISH), are limited by relatively low signal intensity and nonspecific probe binding. Here we present click-amplifying FISH (clampFISH), a method for fluorescence detection of nucleic acids that achieves high specificity and high-gain (>400-fold) signal amplification. ClampFISH probes form a 'C' configuration upon hybridization to the sequence of interest in a double helical manner. Read More

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http://dx.doi.org/10.1038/nbt.4286DOI Listing
November 2018

Q3 2018-The beat goes on.

Nat Biotechnol 2018 Nov;36(11):1033

Nature Biotechnology.

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http://dx.doi.org/10.1038/nbt.4295DOI Listing
November 2018

Wnt is back in drugmakers' sights, but is it druggable?

Authors:
Cormac Sheridan

Nat Biotechnol 2018 Nov;36(11):1028-1029

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http://dx.doi.org/10.1038/nbt1118-1028DOI Listing
November 2018

Recent patents in endonucleases and genome editing.

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Nat Biotechnol 2018 Nov;36(11):1048

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http://dx.doi.org/10.1038/nbt.4300DOI Listing
November 2018

Reply to "Evaluation of immune repertoire inference methods from RNA-seq data".

Nat Biotechnol 2018 Nov;36(11):1035-1036

Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Moscow, Russia.

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http://www.nature.com/doifinder/10.1038/nbt.4296
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November 2018
6 Reads

No added sugar: antibody makers find an upside to 'no fucose'.

Authors:
Ken Garber

Nat Biotechnol 2018 Nov;36(11):1025-1027

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http://dx.doi.org/10.1038/nbt1118-1025DOI Listing
November 2018

Evaluation of immune repertoire inference methods from RNA-seq data.

Nat Biotechnol 2018 Nov;36(11):1034

Department of Biostatistics and Computational Biology, Dana-Farber Cancer Institute, Boston, Massachusetts, USA.

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http://www.nature.com/doifinder/10.1038/nbt.4294
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http://dx.doi.org/10.1038/nbt.4294DOI Listing
November 2018
2 Reads
41.514 Impact Factor

Third-quarter biotech job picture.

Nat Biotechnol 2018 Nov;36(11):1119

Nature Biotechnology.

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http://dx.doi.org/10.1038/nbt.4292DOI Listing
November 2018

First preventive mAb for hereditary angioedema.

Authors:
Joana Osorio

Nat Biotechnol 2018 Nov;36(11):1027

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http://dx.doi.org/10.1038/nbt1118-1027DOI Listing
November 2018
1 Read

Proteomics goes parallel.

Nat Biotechnol 2018 Nov;36(11):1051-1053

Department of Biology, Institute of Molecular Systems Biology, ETH Zurich, Zurich, Switzerland.

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http://dx.doi.org/10.1038/nbt.4288DOI Listing
November 2018

Bandit tusks.

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Nat Biotechnol 2018 Nov;36(11):1032

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http://dx.doi.org/10.1038/nbt1118-1032aDOI Listing
November 2018

The Reporting Items for Patent Landscapes statement.

Nat Biotechnol 2018 Nov;36(11):1043-1047

Nuffield Department of Orthopaedics, Rheumatology and Musculoskeletal Sciences, University of Oxford, Oxford, UK.

The reporting quality of patent landscapes is inadequate. The Reporting Items for Patent Landscapes (RIPL) checklist can improve reporting quality. Read More

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http://www.nature.com/doifinder/10.1038/nbt.4291
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http://dx.doi.org/10.1038/nbt.4291DOI Listing
November 2018
3 Reads

FDA calls for subscription model to pay for anti-infectives.

Authors:
Melanie Senior

Nat Biotechnol 2018 Nov;36(11):1031

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http://dx.doi.org/10.1038/nbt1118-1031DOI Listing
November 2018

Around the world in a month.

Authors:

Nat Biotechnol 2018 Nov;36(11):1032

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http://dx.doi.org/10.1038/nbt1118-1032bDOI Listing
November 2018

Metagenomics meets read clouds.

Nat Biotechnol 2018 Nov;36(11):1049-1051

Faculty of Agricultural and Environmental Sciences, Macdonald Campus, McGill University. Ste-Anne-de-Bellevue, Québec, Canada.

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http://dx.doi.org/10.1038/nbt.4284DOI Listing
November 2018

PODCAST: First rounders: Christoph Lengauer.

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Nat Biotechnol 2018 Nov;36(11):1030

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http://dx.doi.org/10.1038/nbt1118-1030bDOI Listing
November 2018