9,440 results match your criteria Nature Biotechnology[Journal]


Author Correction: Biofortification of field-grown cassava by engineering expression of an iron transporter and ferritin.

Nat Biotechnol 2019 Feb 20. Epub 2019 Feb 20.

Donald Danforth Plant Science Center, St. Louis, MO, USA.

In the version of this article initially published, a relevant work was not cited. The following sentence has been inserted following the sentence ending "Aspergillus phytase" in the third paragraph of the article: "Overexpression of AtIRT1, AtNAS1 and bean FERRITIN in rice resulted in 3.8-fold higher iron and 1. Read More

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http://dx.doi.org/10.1038/s41587-019-0066-6DOI Listing
February 2019

The new liver epidemic.

Authors:
Ken Garber

Nat Biotechnol 2019 Feb 18. Epub 2019 Feb 18.

Ken Garber is a freelance reporter based in Ann Arbor, Michigan, USA.

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http://dx.doi.org/10.1038/s41587-019-0047-9DOI Listing
February 2019

SignalP 5.0 improves signal peptide predictions using deep neural networks.

Nat Biotechnol 2019 Feb 18. Epub 2019 Feb 18.

Department of Bio and Health Informatics, Technical University of Denmark, Kgs Lyngby, Denmark.

Signal peptides (SPs) are short amino acid sequences in the amino terminus of many newly synthesized proteins that target proteins into, or across, membranes. Bioinformatic tools can predict SPs from amino acid sequences, but most cannot distinguish between various types of signal peptides. We present a deep neural network-based approach that improves SP prediction across all domains of life and distinguishes between three types of prokaryotic SPs. Read More

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http://dx.doi.org/10.1038/s41587-019-0036-zDOI Listing
February 2019

Hypoimmunogenic derivatives of induced pluripotent stem cells evade immune rejection in fully immunocompetent allogeneic recipients.

Nat Biotechnol 2019 Feb 18. Epub 2019 Feb 18.

Department of Surgery, Division of Cardiothoracic Surgery, Transplant and Stem Cell Immunobiology-Lab, University of California San Francisco, San Francisco, CA, USA.

Autologous induced pluripotent stem cells (iPSCs) constitute an unlimited cell source for patient-specific cell-based organ repair strategies. However, their generation and subsequent differentiation into specific cells or tissues entail cell line-specific manufacturing challenges and form a lengthy process that precludes acute treatment modalities. These shortcomings could be overcome by using prefabricated allogeneic cell or tissue products, but the vigorous immune response against histo-incompatible cells has prevented the successful implementation of this approach. Read More

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http://dx.doi.org/10.1038/s41587-019-0016-3DOI Listing
February 2019

Regenerating the field of cardiovascular cell therapy.

Nat Biotechnol 2019 Feb 18. Epub 2019 Feb 18.

Institute for Stem Cell Biology and Regenerative Medicine; Ludwig Center for Cancer Stem Cell Biology and Medicine; Departments of Pathology and Developmental Biology, Stanford University School of Medicine, Stanford, CA, USA.

The retraction of >30 falsified studies by Anversa et al. has had a disheartening impact on the cardiac cell therapeutics field. The premise of heart muscle regeneration by the transdifferentiation of bone marrow cells or putative adult resident cardiac progenitors has been largely disproven. Read More

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http://dx.doi.org/10.1038/s41587-019-0042-1DOI Listing
February 2019

Multi-omic measurements of heterogeneity in HeLa cells across laboratories.

Nat Biotechnol 2019 Feb 18. Epub 2019 Feb 18.

Department of Biology, Institute of Molecular Systems Biology, ETH Zurich, Zurich, Switzerland.

Reproducibility in research can be compromised by both biological and technical variation, but most of the focus is on removing the latter. Here we investigate the effects of biological variation in HeLa cell lines using a systems-wide approach. We determine the degree of molecular and phenotypic variability across 14 stock HeLa samples from 13 international laboratories. Read More

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http://www.nature.com/articles/s41587-019-0037-y
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http://dx.doi.org/10.1038/s41587-019-0037-yDOI Listing
February 2019
1 Read

Engineered CRISPR-Cas12a variants with increased activities and improved targeting ranges for gene, epigenetic and base editing.

Nat Biotechnol 2019 Feb 11. Epub 2019 Feb 11.

Molecular Pathology Unit, Massachusetts General Hospital, Charlestown, MA, USA.

Broad use of CRISPR-Cas12a (formerly Cpf1) nucleases has been hindered by the requirement for an extended TTTV protospacer adjacent motif (PAM). To address this limitation, we engineered an enhanced Acidaminococcus sp. Cas12a variant (enAsCas12a) that has a substantially expanded targeting range, enabling targeting of many previously inaccessible PAMs. Read More

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http://dx.doi.org/10.1038/s41587-018-0011-0DOI Listing
February 2019

Proliferation tracing with single-cell mass cytometry optimizes generation of stem cell memory-like T cells.

Nat Biotechnol 2019 Feb 11. Epub 2019 Feb 11.

Department of Pathology, Stanford University, Stanford, CA, USA.

Selective differentiation of naive T cells into multipotent T cells is of great interest clinically for the generation of cell-based cancer immunotherapies. Cellular differentiation depends crucially on division state and time. Here we adapt a dye dilution assay for tracking cell proliferative history through mass cytometry and uncouple division, time and regulatory protein expression in single naive human T cells during their activation and expansion in a complex ex vivo milieu. Read More

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http://dx.doi.org/10.1038/s41587-019-0033-2DOI Listing
February 2019

An injectable bone marrow-like scaffold enhances T cell immunity after hematopoietic stem cell transplantation.

Nat Biotechnol 2019 Feb 11. Epub 2019 Feb 11.

Department of Stem Cell and Regenerative Biology, Harvard University, Cambridge, MA, USA.

Allogeneic hematopoietic stem cell transplantation (HSCT) is a curative treatment for multiple disorders, but deficiency and dysregulation of T cells limit its utility. Here we report a biomaterial-based scaffold that mimics features of T cell lymphopoiesis in the bone marrow. The bone marrow cryogel (BMC) releases bone morphogenetic protein-2 to recruit stromal cells and presents the Notch ligand Delta-like ligand-4 to facilitate T cell lineage specification of mouse and human hematopoietic progenitor cells. Read More

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http://dx.doi.org/10.1038/s41587-019-0017-2DOI Listing
February 2019
4 Reads
41.514 Impact Factor

Ultrafast search of all deposited bacterial and viral genomic data.

Nat Biotechnol 2019 Feb 4;37(2):152-159. Epub 2019 Feb 4.

Wellcome Trust Centre for Human Genetics, University of Oxford, Oxford, UK.

Exponentially increasing amounts of unprocessed bacterial and viral genomic sequence data are stored in the global archives. The ability to query these data for sequence search terms would facilitate both basic research and applications such as real-time genomic epidemiology and surveillance. However, this is not possible with current methods. Read More

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http://dx.doi.org/10.1038/s41587-018-0010-1DOI Listing
February 2019

Capturing sequence diversity in metagenomes with comprehensive and scalable probe design.

Nat Biotechnol 2019 Feb 4;37(2):160-168. Epub 2019 Feb 4.

Broad Institute of MIT and Harvard, Cambridge, MA, USA.

Metagenomic sequencing has the potential to transform microbial detection and characterization, but new tools are needed to improve its sensitivity. Here we present CATCH, a computational method to enhance nucleic acid capture for enrichment of diverse microbial taxa. CATCH designs optimal probe sets, with a specified number of oligonucleotides, that achieve full coverage of, and scale well with, known sequence diversity. Read More

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http://dx.doi.org/10.1038/s41587-018-0006-xDOI Listing
February 2019

Resistance gene cloning from a wild crop relative by sequence capture and association genetics.

Nat Biotechnol 2019 Feb 4;37(2):139-143. Epub 2019 Feb 4.

John Innes Centre, Norwich Research Park, Norwich, UK.

Disease resistance (R) genes from wild relatives could be used to engineer broad-spectrum resistance in domesticated crops. We combined association genetics with R gene enrichment sequencing (AgRenSeq) to exploit pan-genome variation in wild diploid wheat and rapidly clone four stem rust resistance genes. AgRenSeq enables R gene cloning in any crop that has a diverse germplasm panel. Read More

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http://dx.doi.org/10.1038/s41587-018-0007-9DOI Listing
February 2019
41.514 Impact Factor

Asexual crops whet industry appetite.

Authors:
Emily Waltz

Nat Biotechnol 2019 Feb;37(2):109-110

, Nashville, Tennessee, USA.

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http://dx.doi.org/10.1038/s41587-019-0031-4DOI Listing
February 2019

Aquaporin autoimmunity.

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Nat Biotechnol 2019 Feb;37(2):114

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http://dx.doi.org/10.1038/s41587-019-0025-2DOI Listing
February 2019

First Rounders: Chad Womack.

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Nat Biotechnol 2019 Feb;37(2):115

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http://dx.doi.org/10.1038/s41587-019-0020-7DOI Listing
February 2019

Thousands of amyloids may foil Alzheimer's drugs.

Authors:
John Hodgson

Nat Biotechnol 2019 Feb;37(2):114-115

, Cambridge, UK.

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http://dx.doi.org/10.1038/s41587-019-0029-yDOI Listing
February 2019

2018-another biotech banner year.

Nat Biotechnol 2019 Feb;37(2):116-117

Senior Editor at Nature Biotechnology.

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http://dx.doi.org/10.1038/s41587-019-0024-3DOI Listing
February 2019

Digital inhaler for asthma gets FDA nod.

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Nat Biotechnol 2019 Feb;37(2):115

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http://dx.doi.org/10.1038/s41587-019-0028-zDOI Listing
February 2019
2 Reads

Around the world in a month.

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Nat Biotechnol 2019 Feb;37(2):113

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http://dx.doi.org/10.1038/s41587-019-0018-1DOI Listing
February 2019

Plant 'blood' needs FDA OK.

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Nat Biotechnol 2019 Feb;37(2):110

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http://dx.doi.org/10.1038/s41587-019-0027-0DOI Listing
February 2019

India's first-in-class MALT1 blocker deal.

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Nat Biotechnol 2019 Feb;37(2):112

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http://dx.doi.org/10.1038/s41587-019-0026-1DOI Listing
February 2019

Death by inflammation: drug makers chase the master controller.

Authors:
Cormac Sheridan

Nat Biotechnol 2019 Feb;37(2):111-113

, Dublin, Ireland.

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http://dx.doi.org/10.1038/s41587-019-0023-4DOI Listing
February 2019

A human gut bacterial genome and culture collection for improved metagenomic analyses.

Nat Biotechnol 2019 Feb 4;37(2):186-192. Epub 2019 Feb 4.

Host-Microbiota Interactions Laboratory, Wellcome Sanger Institute, Wellcome Genome Campus, Hinxton, UK.

Understanding gut microbiome functions requires cultivated bacteria for experimental validation and reference bacterial genome sequences to interpret metagenome datasets and guide functional analyses. We present the Human Gastrointestinal Bacteria Culture Collection (HBC), a comprehensive set of 737 whole-genome-sequenced bacterial isolates, representing 273 species (105 novel species) from 31 families found in the human gastrointestinal microbiota. The HBC increases the number of bacterial genomes derived from human gastrointestinal microbiota by 37%. Read More

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http://dx.doi.org/10.1038/s41587-018-0009-7DOI Listing
February 2019
1 Read

1,520 reference genomes from cultivated human gut bacteria enable functional microbiome analyses.

Nat Biotechnol 2019 Feb 4;37(2):179-185. Epub 2019 Feb 4.

BGI-Shenzhen, Shenzhen, China.

Reference genomes are essential for metagenomic analyses and functional characterization of the human gut microbiota. We present the Culturable Genome Reference (CGR), a collection of 1,520 nonredundant, high-quality draft genomes generated from >6,000 bacteria cultivated from fecal samples of healthy humans. Of the 1,520 genomes, which were chosen to cover all major bacterial phyla and genera in the human gut, 264 are not represented in existing reference genome catalogs. Read More

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http://www.nature.com/articles/s41587-018-0008-8
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http://dx.doi.org/10.1038/s41587-018-0008-8DOI Listing
February 2019
5 Reads
41.514 Impact Factor

Fresh from the biotech pipeline-2018.

Authors:
Chris Morrison

Nat Biotechnol 2019 Feb;37(2):118-123

Chris Morrison is a freelance reporter based in Yardley, Pennsylvania, USA.

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http://dx.doi.org/10.1038/s41587-019-0021-6DOI Listing
February 2019

The shrinking biotech bazaar.

Authors:

Nat Biotechnol 2019 Feb;37(2):107

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http://dx.doi.org/10.1038/s41587-019-0034-1DOI Listing
February 2019

Fourth-quarter biotech job picture.

Nat Biotechnol 2019 Feb;37(2):194

Senior Editor, Nature Biotechnology, .

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http://dx.doi.org/10.1038/s41587-019-0022-5DOI Listing
February 2019

RNA-based therapies.

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Nat Biotechnol 2019 Feb;37(2):129

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http://dx.doi.org/10.1038/s41587-019-0012-7DOI Listing
February 2019

Precise RNA editing by recruiting endogenous ADARs with antisense oligonucleotides.

Nat Biotechnol 2019 Feb 28;37(2):133-138. Epub 2019 Jan 28.

Interfaculty Institute of Biochemistry, University of Tübingen, Tübingen, Germany.

Site-directed RNA editing might provide a safer or more effective alternative to genome editing in certain clinical scenarios. Until now, RNA editing has relied on overexpression of exogenous RNA editing enzymes or of endogenous human ADAR (adenosine deaminase acting on RNA) enzymes. Here we describe the engineering of chemically optimized antisense oligonucleotides that recruit endogenous human ADARs to edit endogenous transcripts in a simple and programmable way, an approach we call RESTORE (recruiting endogenous ADAR to specific transcripts for oligonucleotide-mediated RNA editing). Read More

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http://www.nature.com/articles/s41587-019-0013-6
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http://dx.doi.org/10.1038/s41587-019-0013-6DOI Listing
February 2019
4 Reads

Biofortification of field-grown cassava by engineering expression of an iron transporter and ferritin.

Nat Biotechnol 2019 Feb 28;37(2):144-151. Epub 2019 Jan 28.

Donald Danforth Plant Science Center, St. Louis, MO, USA.

Less than 10% of the estimated average requirement (EAR) for iron and zinc is provided by consumption of storage roots of the staple crop cassava (Manihot esculenta Crantz) in West African human populations. We used genetic engineering to improve mineral micronutrient concentrations in cassava. Overexpression of the Arabidopsis thaliana vacuolar iron transporter VIT1 in cassava accumulated three- to seven-times-higher levels of iron in transgenic storage roots than nontransgenic controls in confined field trials in Puerto Rico. Read More

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http://dx.doi.org/10.1038/s41587-018-0002-1DOI Listing
February 2019
1 Read

Zinc and iron fortification in cassava.

Nat Biotechnol 2019 Feb;37(2):130-132

International Potato Center, Sub-Saharan Regional Office, Nairobi, Kenya.

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http://dx.doi.org/10.1038/s41587-019-0014-5DOI Listing
February 2019

Reply to 'Errors in long-read assemblies can critically affect protein prediction'.

Nat Biotechnol 2019 Feb;37(2):127-128

School of Life Sciences, University of Nottingham, Nottingham, UK.

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http://dx.doi.org/10.1038/s41587-018-0005-yDOI Listing
February 2019

Errors in long-read assemblies can critically affect protein prediction.

Nat Biotechnol 2019 Feb;37(2):124-126

Genetics and Genomics, The Roslin Institute and Royal (Dick) School of Veterinary Studies, University of Edinburgh, Easter Bush, UK.

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http://dx.doi.org/10.1038/s41587-018-0004-zDOI Listing
February 2019

Publisher Correction: Radioactive drugs emerge from the shadows to storm the market.

Authors:
Elie Dolgin

Nat Biotechnol 2019 Feb;37(2):193

Somerville, Massachusetts, USA.

In the version of this article initially published, Betalutin was described as directing a radioisotope to CD45-expressing B cells; in fact, it directs it to CD37-expressing B cells. The error has been corrected in the HTML and PDF versions of the article. Read More

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http://www.nature.com/articles/s41587-019-0030-5
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http://dx.doi.org/10.1038/s41587-019-0030-5DOI Listing
February 2019
5 Reads

Clonal seeds from hybrid rice by simultaneous genome engineering of meiosis and fertilization genes.

Nat Biotechnol 2019 Jan 4. Epub 2019 Jan 4.

State Key Laboratory of Rice Biology, China National Rice Research Institute, Chinese Academy of Agricultural Sciences, Hangzhou, China.

Heterosis, or hybrid vigor, is exploited by breeders to produce elite high-yielding crop lines, but beneficial phenotypes are lost in subsequent generations owing to genetic segregation. Clonal propagation through seeds would enable self-propagation of F hybrids. Here we report a strategy to enable clonal reproduction of F rice hybrids through seeds. Read More

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http://dx.doi.org/10.1038/s41587-018-0003-0DOI Listing
January 2019
41.514 Impact Factor

Comprehensive identification of RNA-protein interactions in any organism using orthogonal organic phase separation (OOPS).

Nat Biotechnol 2019 Feb 3;37(2):169-178. Epub 2019 Jan 3.

Cambridge Centre for Proteomics, Department of Biochemistry, University of Cambridge, Cambridge, UK.

Existing high-throughput methods to identify RNA-binding proteins (RBPs) are based on capture of polyadenylated RNAs and cannot recover proteins that interact with nonadenylated RNAs, including long noncoding RNA, pre-mRNAs and bacterial RNAs. We present orthogonal organic phase separation (OOPS), which does not require molecular tagging or capture of polyadenylated RNA, and apply it to recover cross-linked protein-RNA and free protein, or protein-bound RNA and free RNA, in an unbiased way. We validated OOPS in HEK293, U2OS and MCF10A human cell lines, and show that 96% of proteins recovered were bound to RNA. Read More

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http://dx.doi.org/10.1038/s41587-018-0001-2DOI Listing
February 2019

UC's latest CRISPR patent.

Nat Biotechnol 2019 Jan;37(1)

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http://dx.doi.org/10.1038/nbt0119-6DOI Listing
January 2019

Yeast solar panels.

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Nat Biotechnol 2019 Jan;37(1):11

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http://dx.doi.org/10.1038/nbt0119-11aDOI Listing
January 2019

A step toward making human oocytes.

Nat Biotechnol 2019 Jan;37(1):24-25

Centre for Reproductive Health, Hudson Institute of Medical Research, and Department of Molecular and Translational Science, Monash University, Clayton, Victoria, Australia.

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http://dx.doi.org/10.1038/nbt.4329DOI Listing
January 2019

Brain modulation and patent law.

Nat Biotechnol 2019 Jan;37(1):18-19

Department of Medical Ethics & Health Policy, University of Pennsylvania, Philadelphia, Pennsylvania, USA.

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http://dx.doi.org/10.1038/nbt.4334DOI Listing
January 2019

Encyclopedia of cells in early pregnancy.

Authors:
Susan Jones

Nat Biotechnol 2019 Jan;37(1):26

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http://www.nature.com/doifinder/10.1038/nbt.4326
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http://dx.doi.org/10.1038/nbt.4326DOI Listing
January 2019
1 Read

Lupus in crisis: as failures pile up, clinicians call for new tools.

Authors:
Elie Dolgin

Nat Biotechnol 2019 Jan;37(1):7-8

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http://dx.doi.org/10.1038/nbt0119-7DOI Listing
January 2019

Recent patents in cancer detection.

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Nat Biotechnol 2019 Jan;37(1):23

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http://dx.doi.org/10.1038/nbt.4340DOI Listing
January 2019

Not 'fake' meat but 'clean'.

Nat Biotechnol 2019 Jan;37(1)

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http://dx.doi.org/10.1038/nbt0119-9bDOI Listing
January 2019

Around the world in a month.

Authors:

Nat Biotechnol 2019 Jan;37(1):10

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http://dx.doi.org/10.1038/nbt0119-10DOI Listing
January 2019

A llama-derived antibody for flu.

Authors:
Saheli Sadanand

Nat Biotechnol 2019 Jan;37(1):27

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http://dx.doi.org/10.1038/nbt.4328DOI Listing
January 2019

An export-only exception to pharmaceutical patents in Europe: should the United States follow suit?

Nat Biotechnol 2019 Jan;37(1):21-22

Harvard Medical School and Brigham & Women's Hospital, Boston, Massachusetts, USA.

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http://www.nature.com/doifinder/10.1038/nbt.4324
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http://dx.doi.org/10.1038/nbt.4324DOI Listing
January 2019
6 Reads