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    Mycobacterium heckeshornense lung infection diagnosed by matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS).
    J Infect Chemother 2018 Mar 1. Epub 2018 Mar 1.
    Department of Respiratory Medicine, The University of Tokyo, Japan.
    Identification of microorganisms by matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) has been widely accepted. However, the significance of MALDI-TOF MS for identifying mycobacteria, particularly rare nontuberculous mycobacteria, has not been established. M. Read More

    Clinical features of infection caused by non-tuberculous mycobacteria: 7 years' experience.
    Infection 2018 Feb 23. Epub 2018 Feb 23.
    Department of Biomedical Sciences, University of Sassari, Sassari, Italy.
    Introduction: Non-tuberculous mycobacteria (NTM) are ubiquitous organisms associated with various infections. The aim of the study was to determine the most relevant clinical characteristics of NTM during the 7-year period.

    Methodology: A retrospective study of NTM infections was conducted between January 2009 and December 2016. Read More

    Mycobacterium xenopi Genotype Associated with Clinical Phenotype in Lung Disease.
    Lung 2018 Apr 18;196(2):213-217. Epub 2018 Jan 18.
    Department of Laboratory Medicine and Pathobiology, University of Toronto, Toronto, ON, Canada.
    Mycobacterium xenopi is responsible for pulmonary disease (PD) in Europe and Canada. Despite its high prevalence and increasing clinical importance, little is known about the genetic diversity of M. xenopi. Read More

    Cavitary lung lesion suspicious for malignancy reveals.
    Respir Med Case Rep 2018 3;23:83-85. Epub 2018 Jan 3.
    GIM Foundation, Little Rock, AR, USA.
    We report the case of a 68-year-old gentleman who presented with musculoskeletal chest pain which appeared suddenly when he bent over with his dog. The chest pain was localized to the left lower chest and increased with movement and deep breathing. The patient did not complain weight loss, night sweat, fever or chill. Read More

    Fatal pulmonary cavitary disease secondary toin a patient with sarcoidosis.
    J Community Hosp Intern Med Perspect 2017 14;7(6):372-377. Epub 2017 Dec 14.
    Pulmonary and Critical Care Department, Interfaith Medical Center, Brooklyn, NY, USA.
    :() has low pathogenicity and usually requires either host immune impairment or structural lung disease to cause clinical disease. Fatal cavitary infection in a patient without immunosuppression is rarely presented.: A 62-year-old female with history of sarcoidosis and hypertension presented with cough, fever and dyspnea for one week. Read More

    Evaluation of PyroMark Q24 pyrosequencing as a method for the identification of mycobacteria.
    Diagn Microbiol Infect Dis 2018 Jan 15;90(1):35-39. Epub 2017 Sep 15.
    Department of Mycobacterium Reference and Research, the Research Institute of Tuberculosis, Japan Anti-Tuberculosis Association, Kiyose, Japan; Basic Mycobacteriology, Graduate School of Biomedical Science, Nagasaki University, Nagasaki, Japan.
    We evaluated PyroMark Q24 (QIAGEN) pyrosequencing as a method for the identification of mycobacteria, with potential application in clinical practice. Sequence data from the hypervariable region A of the 16S rRNA gene (43 and 35bp sequences) were obtained using PyroMark Q24, and a similarity search was performed automatically with PyroMark IdentiFire software. Of the 148 mycobacterial type strains tested, 138 (93. Read More

    The new phylogeny of the genus Mycobacterium: The old and the news.
    Infect Genet Evol 2017 Dec 11;56:19-25. Epub 2017 Oct 11.
    Emerging Bacterial Pathogens Unit, IRCCS San Raffaele Scientific Institute, Milano, Italy. Electronic address:
    Background: Phylogenetic studies of bacteria have been based so far either on a single gene (usually the 16S rRNA) or on concatenated housekeeping genes. For what concerns the genus Mycobacterium these approaches support the separation of rapidly and slowly growing species and the clustering of most species in well-defined phylogenetic groups. The advent of high-throughput shotgun sequencing leads us to revise conventional taxonomy of mycobacteria on the light of genomic data. Read More

    Transmission ofto a pet albino ferret () from a domestic aquarium.
    Vet Rec 2017 Aug 3. Epub 2017 Aug 3.
    Beaumont Sainsbury Animal Hospital, Royal Veterinary College, London, UK.
    A three-year-old ferret presented with a three-month history of rapid clinical deterioration necessitating euthanasia shortly after initial veterinary assessment. Postmortem PCR testing confirmedwhich is most commonly identified in amphibians, reptiles and aquatic life. Infection of a captive-bred domestic ferret is highly unusual. Read More

    Mannich Ketones as Possible Antimycobacterial Agents.
    Arch Pharm (Weinheim) 2017 Sep 28;350(9). Epub 2017 Jul 28.
    Faculty of Medicine, Department of Biochemistry and Medical Chemistry, University of Pécs, Pécs, Hungary.
    Twenty-three known unsaturated and fused Mannich ketones and their reduced derivatives (amino alcohols) were selected for an antituberculotic study. They were screened against several mycobacterial strains including Mycobacterium tuberculosis, M. xenopi, and M. Read More

    Design, synthesis and evaluation of indole-2-carboxamides with pan anti-mycobacterial activity.
    Bioorg Med Chem 2017 07 8;25(14):3746-3755. Epub 2017 May 8.
    Department of Pharmacy Sciences, Creighton University, 2500 California Plaza, Omaha, NE 68178, USA. Electronic address:
    Current treatment regimens for non-tuberculous mycobacteria (NTM) and tuberculosis (TB) generally require long duration of therapy with multiple drugs, some of which are broad spectrum antibiotics. Despite some advances in antimicrobial compounds, there remains a need in therapy for antibiotics with specific mycobacterial targets. It has been shown that MmpL3 is an essential transporter required for the translocation of mycolic acids to the mycobacterial cell envelope. Read More

    Expression, purification, and characterization of recombinant 8 kDa gelsolin fragment.
    Protein Expr Purif 2017 Jul 29;135:33-36. Epub 2017 Apr 29.
    The State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing, Jiangsu, 163 Xianlin Avenue, 210046, China; Changzhou High-Tech Research Institute of Nanjing University and Jiangsu Target Pharma Laboratories Inc., Changzhou, Jiangsu, 213164, China. Electronic address:
    A mutation (D187N/Y) in human plasma gelsolin (GSN) leads to the generation of an 8 kDa GSN fragment (8 kDa-GSN), and consequently causes the familial amyloidosis of Finnish type. Because of its faster kinetics of amyloid formation under physiologically relevant conditions, 8 kDa-GSN is used to explore gelsolin amyloidosis and screen small molecules that can disaggregate amyloids. However, the synthetic 8 kDa-GSN is expensive, and substantial quantities of 8 kDa-GSN are needed for the screen. Read More

    Microbiological and Clinical Outcomes of Treating Non-Mycobacterium Avium Complex Nontuberculous Mycobacterial Pulmonary Disease: A Systematic Review and Meta-Analysis.
    Chest 2017 07 28;152(1):120-142. Epub 2017 Apr 28.
    Institute for Health Service Research in Dermatology and Nursing, University Medical Center Hamburg-Eppendorf, Hamburg, Germany; Institution for Statutory Accident Insurance and Prevention in the Health and Welfare Services (BGW), Hamburg, Germany.
    Background: Pulmonary disease caused by nontuberculous mycobacteria (NTM) is steadily increasing worldwide.

    Methods: A systematic review of non-Mycobacterium avium complex studies published prior to October 2016 was conducted with respect to microbiological and clinical outcomes of current treatment regimens.

    Results: We retrieved 352 citations, which yielded 24 studies eligible for evaluation. Read More

    The volatile molecule signature of four mycobacteria species.
    J Breath Res 2017 Jun 29;11(3):031002. Epub 2017 Jun 29.
    Thayer School of Engineering, Dartmouth College, 14 Engineering Drive, Hanover, NH 03755, United States of America.
    Mycobacteria are the leading cause of death from infectious disease worldwide and limitations in current diagnostics are hampering control efforts. In recent years, the use of small volatile molecules as diagnostic biomarkers for mycobacteria has shown promise for use in the rapid analysis of in vitro cultures as well as ex vivo diagnosis using breath or sputum. In this study, 18 strains from four mycobacteria species (Mycobacterium avium, M. Read More

    Structure of the mycobacterial ESX-5 type VII secretion system membrane complex by single-particle analysis.
    Nat Microbiol 2017 Apr 10;2:17047. Epub 2017 Apr 10.
    Vrije Universiteit Amsterdam, De Boelelaan 1108, 1081 HZ Amsterdam, The Netherlands.
    Mycobacteria are characterized by their impermeable outer membrane, which is rich in mycolic acids. To transport substrates across this complex cell envelope, mycobacteria rely on type VII (also known as ESX) secretion systems. In Mycobacterium tuberculosis, these ESX systems are essential for growth and full virulence and therefore represent an attractive target for anti-tuberculosis drugs. Read More

    Species-Specific Risk Factors, Treatment Decisions, and Clinical Outcomes for Laboratory Isolates of Less Common Nontuberculous Mycobacteria in Washington State.
    Ann Am Thorac Soc 2017 Jul;14(7):1129-1138
    1 Department of Medicine, School of Medicine and.
    Rationale: Nontuberculous mycobacteria (NTM) are a diverse group of environmental organisms that infrequently cause human disease. Understanding of the epidemiologic and clinical characteristics associated with NTM disease is needed to refine diagnostic and treatment strategies, particularly among the less commonly isolated species.

    Objectives: To improve knowledge of geographic variance of NTM species, to correlate detailed clinical information with isolation of specific NTM, and to examine the decision to treat and outcomes for specific NTM. Read More

    [Distribution and drug resistance of nontuberculousin Beijing].
    Zhonghua Jie He He Hu Xi Za Zhi 2017 Mar;40(3):210-214
    Central Laboratory of Beijing Research Institute for Tuberculosis Control, Beijing 100035, China.
    To analyze the distribution and drug resistance of nontuberculous mycobacteria(NTM) in Beijing.Using PCR-fluorescence probe method we identified 1 552 mycobacterial isolates in 2009 and 1 553 mycobacterial isolates in 2013, which were stored by Beijing Research Institute for Tuberculosis Control.All identified NTM strains were confirmed by 16S rRNA gene sequencing, and drug sensitivity testing was performed by using 1% ratio method. Read More

    Pulmonary Nontuberculous Mycobacteria-Associated Deaths, Ontario, Canada, 2001-2013.
    Emerg Infect Dis 2017 Mar;23(3):468-476
    Survival implications of nontuberculous mycobacterial pulmonary disease (NTM-PD) and NTM pulmonary isolation without disease (NTM-PI) are unclear. To study deaths associated with NTM-PD and NTM-PI and differences in survival between them, we conducted a population-based cohort study of persons with microbiologically defined NTM-PD or NTM-PI diagnosed during 2001-2013 in Ontario, Canada. We used propensity score matching and Cox proportional hazards models to compare survival. Read More

    Nontuberculous Mycobacterial Infections in a French Hospital: A 12-Year Retrospective Study.
    PLoS One 2016 13;11(12):e0168290. Epub 2016 Dec 13.
    Service de maladies infectieuses et tropicales, Centre Hospitalier Universitaire de Bordeaux, Bordeaux, France.
    Background: Nontuberculous mycobacteria (NTM) are environmental organisms associated with a range of infections. Reports of NTM epidemiology are mainly focused on pulmonary infections and isolations, and extrapulmonary infections are less frequently described.

    Methods: We conducted a retrospective study of NTM infections at the Bordeaux University Hospital, France, between January 2002 and December 2013. Read More

    Other Slow-Growing Nontuberculous Mycobacteria.
    Microbiol Spectr 2016 11;4(6)
    Infectious Diseases Section, VA Medical Center, Omaha, NE 68105.
    The list of clinically important slow-growing nontuberculous mycobacteria (NTM) continues to expand as new species are identified and older ones are found to be pathogenic. Based on pigment production, the strains may be classified as photochromogenic, scotochromogenic, or unpigmented. Some of these organisms are not newly discovered but have heretofore been considered virtually nonpathogenic. Read More

    Factors which influence treatment initiation for pulmonary non-tuberculous mycobacterium infection in HIV negative patients; a multicentre observational study.
    Respir Med 2016 11 6;120:101-108. Epub 2016 Oct 6.
    London North West Healthcare NHS Trusts, London, UK.
    Background: Clinical, radiological and microbiological criteria inform diagnosis of pulmonary Non-Tuberculous Mycobacteria (NTM) disease and treatment decisions. This multicentre, review aims to characterise NTM disease meeting ATS/IDSA criteria and define factors associated with initiation of treatment.

    Methods: Sputum samples growing NTM from 5 London hospitals between 2010 and 2014 were identified. Read More

    Non-tuberculous mycobacteria: epidemiological pattern in a reference laboratory and risk factors associated with pulmonary disease.
    Epidemiol Infect 2017 02 2;145(3):515-522. Epub 2016 Nov 2.
    Unit of Infectious and Tropical Diseases,Careggi Hospital,Florence,Italy.
    The diseases caused by non-tuberculous mycobacteria (NTM), in both AIDS and non-AIDS populations, are increasingly recognized worldwide. Although the American Thoracic Society published the guidelines for diagnosis of NTM pulmonary disease (NTM-PD), the diagnosis is still difficult. In the first part of the study, we collected data on NTM isolates in the Mycobacteriology Laboratory of Careggi Hospital (Florence, Italy) and analysed the epidemiological data of NTM isolates. Read More

    Comparison of MALDI-TOF MS, nucleic acid hybridization and the MPT64 immunochromatographic test for the identification of M. tuberculosis and non-tuberculosis Mycobacterium species.
    New Microbiol 2016 Oct 23;39(4):259-263. Epub 2016 Aug 23.
    Marmara University, Faculty of Medicine, Department of Medical Microbiology, Istanbul, Turkey.
    Mycobacteria are an important cause of morbidity in humans. Rapid and accurate mycobacterial identification is important for improving patient outcomes. However, identification of Mycobacterium species is not easy, due to the slow and fastidious growth of mycobacteria. Read More

    Miliary pulmonary nodules due to Mycobacterium xenopi in a steroid-induced immunocompromised patient successfully treated with chemotherapy: a case report.
    BMC Pulm Med 2016 Jun 10;16(1):92. Epub 2016 Jun 10.
    Division of Pulmonary Medicine, National Hospital Organization Kochi Hospital, 1-2-25 Asakuranishimachi, Kochi, 780-8077, Japan.
    Background: Mycobacterium xenopi-infected patients have a high prevalence of pulmonary cavities and nodules. However, the clinical course for patients with miliary nodules due to M. xenopi has not yet been reported. Read More

    Pulmonary Disease Caused by Non-Tuberculous Mycobacteria.
    Respiration 2016 21;91(5):386-402. Epub 2016 May 21.
    Division of Clinical Infectious Diseases, Research Center Borstel, Borstel, Germany.
    Non-tuberculous mycobacteria (NTM) include more than 160 ubiquitous, environmental, acid-fast-staining bacterial species, some of which may cause disease in humans. Chronic pulmonary infection is the most common clinical manifestation. Although patients suffering from chronic lung diseases are particularly susceptible to NTM pulmonary disease, many affected patients have no apparent risk factors. Read More

    Vertebral osteomyelitis caused by non-tuberculous mycobacteria: Predisposing conditions and clinical characteristics of six cases and a review of 63 cases in the literature.
    Infect Dis (Lond) 2016 Jul 22;48(7):509-16. Epub 2016 Mar 22.
    a Department of Internal Medicine , Seoul National University College of Medicine , Seoul , Republic of Korea ;
    Background Several case series have reported on clinical and radiographic characteristics of patients with vertebral osteomyelitis (VO) caused by non-tuberculous mycobacteria (NTM). However, only a few patients were included, and systematic reviews are still lacking. The aim of this study was to update and summarise the pre-disposing conditions, clinical and radiographic characteristics of such cases due to NTM. Read More

    Update on pulmonary disease due to non-tuberculous mycobacteria.
    Int J Infect Dis 2016 Apr 11;45:123-34. Epub 2016 Mar 11.
    Stanley Ho Centre for Emerging Infectious Diseases, The Chinese University of Hong Kong, Hong Kong, China.
    Non-tuberculous mycobacteria (NTM) are emerging worldwide as significant causes of chronic pulmonary infection, posing a number of challenges for both clinicians and researchers. While a number of studies worldwide have described an increasing prevalence of NTM pulmonary disease over time, population-based data are relatively sparse and subject to ascertainment bias. Furthermore, the disease is geographically heterogeneous. Read More

    Macrocolonies (Granules) Formation as a Cause of False-Negative Results in the MGIT 960 System: Cause Analysis and Correlation with Mycobacterial Species.
    Biomed Res Int 2015 4;2015:501847. Epub 2015 Nov 4.
    National Clinical Laboratory on Tuberculosis, Beijing Key laboratory on Drug-Resistant Tuberculosis Research, Beijing Chest Hospital, Capital Medical University, Beijing Tuberculosis and Thoracic Tumor Institute, Beijing 101149, China.
    Background: The viable mycobacterial bacilli can sometimes form granules in the Mycobacterium Growth Indicator Tube (MGIT) to produce instrument-negative outcomes when BACTEC MGIT 960 culture is performed. The cause of this phenomenon has never been analyzed.

    Methods: Thirty-one instrument-negative, granule presenting MGIT vials were collected for conducting acid-fast staining and also liquid and solid subculture. Read More

    Direct identification of mycobacteria from liquid media using a triplex real-time PCR coupled with pyrosequencing method.
    J Microbiol Methods 2015 Dec 22;119:83-6. Epub 2015 Oct 22.
    Department of Laboratory Medicine, Gangneung Asan Hospital, University of Ulsan College of Medicine, Gangneung, Republic of Korea.
    Culture in enriched broth, as well as on a solid medium, is recommended for primary isolation of mycobacteria. With the introduction of liquid mycobacterial culture methods, a substantial workload regarding the identification of culture-recovered mycobacterial species, particularly Mycobacterium tuberculosis complex (MTC), has been imposed on our laboratory. We thus developed a triplex, real-time PCR coupled with pyrosequencing assay that can directly identify mycobacterial species from liquid media, which can reduce the workload. Read More

    Osteomyelitis Because of Mycobacterium Xenopi in an Immunocompetent Child.
    Pediatr Infect Dis J 2016 Jan;35(1):110-3
    From the *Department of General Pediatrics, Adolescent Medicine and Neonatology, University Medical Center Freiburg, Freiburg, Germany; †Center for Chronic Immunodeficiency (CCI), University Medical Center Freiburg and University of Freiburg, Freiburg, Germany; and ‡Institute for Surgical Pathology, University Medical Center Freiburg, Freiburg, Germany.
    We present the case of a 6-year-old, immunocompetent boy with chronic osteomyelitis of the calcaneus caused by Mycobacterium xenopi. Of note, typical histopathology was not visible on the first biopsy and developed only later over a period of 6 weeks, highlighting the difficult differential diagnosis of osteomyelitis caused by nontuberculous mycobacteria. Read More

    Engineering versatile protein expression systems mediated by inteins in Escherichia coli.
    Appl Microbiol Biotechnol 2016 Jan 17;100(1):255-62. Epub 2015 Sep 17.
    Division of Life Science, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong, China.
    We have recently employed an intein, Saccharomyces cerevisiae vascular membrane ATPase (VMA), in conjunction with efficient expression and secretory functions formed between the ompA leader sequence and the human epidermal growth factor (EGF) gene (fused at the 5' end of VMA), and the human basic fibroblast growth factor (bFGF) gene (fused at the 3' end of VMA), to engineer an efficient intein-based Escherichia coli system for high-level co-expression of EGF and bFGF as authentic mature products. Both products were found not only excreted to the culture medium but also located, surprisingly, in the cytoplasm (Kwong and Wong 2013). In this study, we employed two structurally varied inteins, VMA and Mycobacterium xenopi GyraseA (GyrA), and further demonstrated that despite acting alone, both VMA and GyrA were able to mediate successful co-expression of two widely different proteins, EGF and an endoglucanase (Eng) in E. Read More

    Time-kill kinetics of slowly growing mycobacteria common in pulmonary disease.
    J Antimicrob Chemother 2015 Oct 4;70(10):2838-43. Epub 2015 Jul 4.
    Department of Medical Microbiology, Radboud University Medical Center, Nijmegen, The Netherlands Department of Medical Microbiology and Infectious Diseases, Erasmus Medical Center, Rotterdam, The Netherlands.
    Objectives: This study aimed to provide basic pharmacodynamic information for key antibiotics used to treat Mycobacterium avium and Mycobacterium xenopi pulmonary disease.

    Methods: M. avium subspecies hominissuis IWGMT49 and M. Read More

    Epidemiology of human pulmonary infection with nontuberculous mycobacteria: a review.
    Clin Chest Med 2015 Mar 6;36(1):13-34. Epub 2014 Nov 6.
    Division of Respirology, Department of Medicine, University of Toronto and Toronto Western Hospital, 399 Bathurst Street, 7E-452, Toronto, ON M5T 2S8, Canada.
    Population-based data have documented a worldwide increase in the prevalence of human nontuberculous mycobacterial (NTM) infections since 2000. Mycobacterium avium complex is predominant in North America and East Asia, whereas in regions within Europe, M kansasii, M xenopi, and M malmoense are more common. Host factors important to the current epidemiology of NTM pulmonary disease include thoracic skeletal abnormalities, rheumatoid arthritis, and use of immunomodulatory drugs. Read More

    Evaluation of the Speed-Oligo Mycobacteria assay for the identification of nontuberculous mycobacteria.
    J Med Microbiol 2015 Mar 16;64(Pt 3):283-7. Epub 2015 Jan 16.
    Laboratory of Microbiology, Department of Biochemistry and Microbiology, Ghent University, Gent, Belgium.
    Nontuberculous mycobacteria (NTM) causing human infectious disease have become increasingly common. Rapid and accurate identification to the species level is, therefore, critical. The Speed-Oligo Mycobacteria assay is an oligochromatographic method that was made available recently for the identification and differentiation of mycobacteria. Read More

    Comparative analysis of the effectiveness of C-terminal cleavage intein-based constructs in producing a recombinant analog of anophelin, an anticoagulant from Anopheles albimanus.
    Appl Biochem Biotechnol 2015 Mar 13;175(5):2468-88. Epub 2014 Dec 13.
    Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, 16/10 Miklukho-Maklaya St., Moscow, Russia, 117997,
    Production of small recombinant peptides by expressing them as fusion proteins, with subsequent proteolytic or chemical cleavage of the latter, is a widespread approach in modern biotechnology. An alternative method is to produce such peptides as self-cleaving fusion proteins with inteins. To date, only a small proportion of known inteins have been used for this purpose, and analysis of other inteins for the ability to cleave off the target polypeptide can significantly expand the range of intein-based transgenic constructs available to researchers. Read More

    Risk factors for mortality in patients with pulmonary infections with non-tuberculous mycobacteria: a retrospective cohort study.
    Respir Med 2015 Jan 12;109(1):137-45. Epub 2014 Nov 12.
    Department of Respiratory Medicine, Maastricht University Medical Center, P. Debyelaan 25, 6202 AZ Maastricht, The Netherlands. Electronic address:
    Background: Infections with non-tuberculous mycobacteria (NTM) represent an increasing problem. Their clinical relevance is still largely unknown as well as predictors for mortality in affected patients. The objective was to describe prevalence and clinical relevance of different NTM and to identify risk factors for mortality. Read More

    [Identification of Mycobacterium spp. isolates using matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry (MALDI-TOF MS)].
    Epidemiol Mikrobiol Imunol 2014 Sep;63(3):196-9
    Study Objective: Matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry (MALDI-TOF MS) has recently been widely used in diagnostic microbiological laboratories. It is a cheap and rapid method for the identification of bacteria and micromycetes. Apart from this purpose, it is also used for the detection of antibiotic resistance mechanisms. Read More

    Synthesis and anti-mycobacterial activity of new 4-thiazolidinone and 1,3,4-oxadiazole derivatives of isoniazid.
    Acta Pol Pharm 2014 Sep-Oct;71(5):763-70
    A new series of 4-thiazolidinone (3a-e) and 1,3,4-oxadiazole (4a-e) derivatives of isoniazid were synthesized and evaluated for their in vitro anti-mycobacterial activity. The structures of the compounds were confirmed on the basis of spectral data and elemental analysis. Some compounds showed interesting activity against four Mycobacterium strains: M. Read More

    Multilocus sequence typing of Mycobacterium xenopi.
    J Clin Microbiol 2014 Nov 10;52(11):3973-7. Epub 2014 Sep 10.
    Public Health Ontario Laboratories, Public Health Ontario, Toronto, ON, Canada University of Toronto, Department of Laboratory Medicine and Pathobiology, Toronto, ON, Canada
    Mycobacterium xenopi is an opportunistic mycobacterial pathogen of increasing clinical importance. Surveillance of M. xenopi is hampered by the absence of tools for genotyping and molecular epidemiology. Read More

    Highly Deviated Asymmetric Division in Very Low Proportion of Mycobacterial Mid-log Phase Cells.
    Open Microbiol J 2014 30;8:40-50. Epub 2014 May 30.
    Department of Microbiology and Cell Biology, Indian Institute of Science, Bangalore - 560012,Karnataka,India.
    In this study, we show that about 20% of the septating Mycobacterium smegmatis and Mycobacterium xenopi cells in the exponential phase populationdivideasymmetrically, with an unusually high deviation (17 ± 4%) in the division site from the median, to generate short cells and long cells, thereby generating population heterogeneity. This mode of division is very different from the symmetric division of themajority (about 80%) of the septating cells in the Mycobacterium smegmatis, Mycobacterium marinum, and Mycobacterium bovis BCG exponential phase population, with 5-10% deviation in the division site from the mid-cell site, as reported by recent studies. The short cells and the long cells further grew and divided to generate a population. Read More

    Non-tuberculous mycobacterial infections at San Francisco General Hospital.
    Clin Respir J 2015 Oct 10;9(4):436-42. Epub 2014 Jun 10.
    Division of Pulmonary and Critical Care Medicine, University of California, San Francisco, CA, USA.
    Background And Aims: The epidemiology of non-tuberculous mycobacteria (NTM) infection is not well defined. We evaluated the trends in incidence of NTM infections at San Francisco General Hospital (SFGH), a large metropolitan county hospital.

    Methods: We performed a retrospective review of microbiologic and clinical records of all patients with a positive NTM culture reported from 1993 to 2001. Read More

    Inventory study of non-tuberculous mycobacteria in the European Union.
    BMC Infect Dis 2014 Feb 6;14:62. Epub 2014 Feb 6.
    European Centre for Disease Prevention and Control, Stockholm, Sweden.
    Background: Since non-tuberculous mycobacteria (NTM) disease is not notifiable in most European Union (EU) and European Economic Area (EEA) countries, the epidemiological situation of the >150 NTM species is largely unknown. We aimed to collect data on the frequency of NTM detection and NTM species types in EU/EEA countries.

    Methods: Officially nominated national tuberculosis reference laboratories of all EU/EEA countries were asked to provide information on: laboratory routines for detection and identification of NTM, including drug sensitivity testing (DST) methods; data on the number and type of NTM species identified; coverage and completeness of the provided data on NTM; type and number of human specimens tested for NTM; and number of specimens tested for Mycobacterium tuberculosis complex and NTM. Read More

    Nontuberculous mycobacteria in guadeloupe, martinique, and French Guiana from 1994 to 2012.
    Tuberc Res Treat 2013 18;2013:472041. Epub 2013 Dec 18.
    WHO Supranational TB Reference Laboratory, Tuberculosis & Mycobacteria Unit, Institut Pasteur de la Guadeloupe, Morne Jolivière, BP 484, 97183 Les Abymes, France.
    Nontuberculous mycobacteria (NTM) are ubiquitous environmental organisms able to cause severe opportunistic human infections. Their distribution patterns are subject to geographical variations. This study describes their isolation frequencies from clinical specimen in the three French overseas departments of the Americas, namely, Guadeloupe, Martinique, and French Guiana during 1994-2012. Read More

    Comparison of the spectrum of radiologic and clinical manifestations of pulmonary disease caused by Mycobacterium avium complex and Mycobacterium xenopi.
    Can Assoc Radiol J 2014 Aug 8;65(3):207-13. Epub 2013 Dec 8.
    Department of Medicine, Division of Respirology, University Health Network, Toronto, Ontario, Canada; Department of Medicine, Division of Respirology, University of Toronto, Toronto, Ontario, Canada.
    Aim: Mycobacterium xenopi is described with upper lobe cavitation ("fibrocavitary" pattern), whereas the Mycobacterium avium complex (MAC) is described with bronchiectasis and centrilobular nodules ("nodular bronchiectasis"). We retrospectively described and compared computed tomography (CT) chest manifestations of disease caused by MAC and M xenopi.

    Materials And Methods: We reviewed patients who had either MAC or M xenopi lung disease and who had CTs between January 2002 and December 2003. Read More

    Distribution of nontuberculous Mycobacteria strains.
    Ann Clin Microbiol Antimicrob 2013 Nov 21;12:33. Epub 2013 Nov 21.
    Department of Medical Microbiology, Faculty of Medicine, Ondokuz Mayis University, Samsun, Turkey.
    Aim: Mycobacteria other than tuberculosis (MOTT) cause increasingly serious infections especially in immunosuppressive patients by direct transmission from the environment or after colonization. However, identification of these species is difficult because of the cost and difficulties in defining to species level. Identification and distribution of these species can help clinician in the choice of treatment. Read More

    Thin layer chromatography characterization of ELISA specific glycolipids antigens immunomagnetically purified from environmental mycobacteria.
    Roum Arch Microbiol Immunol 2013 Apr-Jun;72(2):105-20
    Cantacuzino National Institute of Research-Development for Microbiology and Immunology, Mycobacterial Antigens Department, Bucharest, Romania.
    Thin layer chromatography (TLC) could easily and rapidly evidentiate the qualitative differences between glycolipids (GLs). Different immunomagnetically purified mycobacterial GLs have been compared using TLC, in order to choose the most appropriate antigens to be utilized in ELISA. The GLs were purified from environmental mycobacteria (EM) (M. Read More

    Identification of non-tuberculous mycobacteria from clinical samples.
    New Microbiol 2013 Oct 1;36(4):409-11. Epub 2013 Oct 1.
    Department of Biomedical Sciences University of Sassari.
    Non-tuberculous mycobacterial infections cause morbidity worldwide. NTM are considered opportunistic pathogens, and several species have been associated with human disease which has typically pulmonary, skin and soft tissue, lymphatic or disseminated presentation. This study evaluated the distribution of non-tuberculous mycobacteria in Sardinia. Read More

    Drug treatment of pulmonary nontuberculous mycobacterial disease in HIV-negative patients: the evidence.
    Expert Rev Anti Infect Ther 2013 Oct;11(10):1065-77
    Department of Medical Microbiology, Radboud University Medical Center, Nijmegen, The Netherlands.
    Pulmonary disease (PD) caused by nontuberculous mycobacteria is an emerging infection mainly in countries where the incidence of tuberculosis is in decline. It affects an elderly population, often with underlying chronic lung diseases, but its epidemiology shows significant regional variation. Guidelines and recommendations for treatment of these infections exist, but build strongly on expert opinion, as very few good quality clinical trials have been performed in this field. Read More

    Phytochemical, antimicrobial and antiprotozoal evaluation of Garcinia mangostana pericarp and α-mangostin, its major xanthone derivative.
    Molecules 2013 Sep 2;18(9):10599-608. Epub 2013 Sep 2.
    Department of Pharmacognosy, College of Pharmacy, King Saud University, P.O. Box 2457, Riyadh 11451, Saudi Arabia.
    Five xanthone derivatives and one flavanol were isolated from the dichloromethane extract of Garcinia mangostana. Dichloromethane, ethyl acetate extract and the major xanthone (α-mangostin) were evaluated in vitro against erythrocytic schizonts of Plasmodium falciparum, intracellular amastigotes of Leishmania infantum and Trypanosoma cruzi and free trypomastigotes of T. brucei. Read More

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