109,721 results match your criteria Mycobacterium Avium-Intracellulare Infection


Allicin enhances antimicrobial activity of macrophages during Mycobacterium tuberculosis infection.

J Ethnopharmacol 2018 Dec 8. Epub 2018 Dec 8.

Special Centre for Molecular Medicine, Jawaharlal Nehru University, New Delhi, India. Electronic address:

Ethnopharmacological Relevance: The emergence of drug-resistant Mycobacterium tuberculosis (M.tb) strains has severely hampered global efforts towards tuberculosis (TB) eradication. The internationally accepted therapy "Directly Observed Treatment Short-course (DOTS)" is lengthy, and incorporates risks for the generation of drug-resistant M. Read More

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December 2018

Structures and activities of widely conserved small prokaryotic Aminopeptidases P clarify classification of M24B peptidases.

Proteins 2018 Dec 11. Epub 2018 Dec 11.

High Pressure and Synchrotron Radiation Physics Division, Bhabha Atomic Research Centre, Mumbai, India.

The proline-specific M24B peptidases that cleave Xaa-Pro iminopeptide bond in dipeptides are called prolidases while those that preferentially cleave this bond at the N-termini of longer peptides are called aminopeptidases-P. Escherichia coli and other bacteria have small aminopeptidases-P (36-39 kDa) which are considerably diverged from the canonical aminopeptidase-P of E. coli (50 kDa). Read More

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December 2018

Removal Efficacy of Opportunistic Pathogens and Bacterial Community Dynamics in Two Drinking Water Treatment Trains.

Small 2018 Dec 7:e1804436. Epub 2018 Dec 7.

Department of Thoracic Surgery, Shanghai Pulmonary Hospital, Tongji University, Shanghai, 200433, China.

Drinking water treatment processes (DWTPs) impact pathogen colonization and microbial communities in finished water; however, their efficacies against opportunistic pathogens are not fully understood. In this study, the effects of treatment steps on the removal of Legionella spp., Legionella pneumophila, nontuberculous mycobacteria, Mycobacterium avium, and two amoeba hosts (Vermamoeba vermiformis, Acanthamoeba) are evaluated in two parallel trains of DWTPs equipped with different pretreatment units. Read More

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December 2018

An interferon gamma release assay specific for Histoplasma capsulatum to detect asymptomatic infected individuals: A proof of concept study.

Med Mycol 2018 Dec 11. Epub 2018 Dec 11.

Grupo de Micología Médica y Experimental, Corporación para Investigaciones Biológicas (CIB), Medellín, Colombia.

Histoplasmosis is the most common endemic mycosis in the Americas. Currently, there is no laboratory test capable to detect subclinical or latent infections by Histoplasma capsulatum (Hc), which might develop as severe infections in immunocompromised individuals. For the first time to our knowledge, we explore the suitability of an interferon gamma release assay (IGRA) to detect latent Hc infection in asymptomatic individuals. Read More

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December 2018

The Expression of lncRNA NEAT1 in Human Tuberculosis and Its Antituberculosis Effect.

Biomed Res Int 2018 11;2018:9529072. Epub 2018 Nov 11.

Intensive Care Unit, The First Affiliated Hospital of Nanchang University, Nanchang, 330006 Jiangxi, China.

Increasing evidence suggests that lncRNA is important in innate immune responses. Recent study has demonstrated that lncRNA NEAT1 (which has two subtypes: NEAT1_1 and NEAT1_2) nuclear-enriched abundant transcript 1 (NEAT1) is essential in immune regulation, but the expression and clinical significance in tuberculosis are still unclear. In this work, we aimed to discuss the expression and clinical significance of NEAT1 in tuberculosis patients. Read More

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November 2018

Line-probe assay and molecular typing reveal a potential drug resistant clone of in Ethiopia.

Trop Dis Travel Med Vaccines 2018 4;4:15. Epub 2018 Dec 4.

1Armauer Hansen Research Institute, Jima Road, Addis Ababa, Ethiopia.

Background: Antimicrobial resistance is a global concern of increasing significance. Multidrug resistant tuberculosis (MDR-TB) is spreading worldwide. It is important to monitor trends of antimycobacterial resistance. Read More

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December 2018

The bovine tuberculosis cluster in north County Sligo during 2014-16.

Ir Vet J 2018 28;71:24. Epub 2018 Nov 28.

2Centre for Veterinary Epidemiology and Risk Analysis, UCD School of Veterinary Medicine, University College Dublin, Belfield, Dublin, D04 W6F6 Ireland.

Background: Bovine tuberculosis (bTB, caused by infection with ) is endemic in the Irish cattle population, and the subject of a national eradication programme since the late 1950s. During 2014, a substantial area-level bTB outbreak developing in north County Sligo, necessitating the need for an enhanced response. This paper describes this outbreak, the response that was undertaken and some lessons learned. Read More

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November 2018

Development of multiplex PCR and multi-color fluorescent in situ hybridization (-FISH) coupled protocol for detection and imaging of multi-pathogens involved in inflammatory bowel disease.

Gut Pathog 2018 5;10:51. Epub 2018 Dec 5.

Division of Molecular Microbiology, Burnett School of Biomedical Sciences, University of Central Florida College of Medicine, 4110 Libra Drive, Orlando, FL USA.

Background: Several pathogens have been debated to play a role in inflammatory bowel disease (IBD) including Crohn's disease (CD). None of these pathogens have been investigated together in same clinical samples. We developed a multiplex PCR and multi-color fluorescent in situ hybridization (-FISH) protocols for simultaneous detection of CD-associated pathogens including subspecies (MAP), , and adherent-invasive strain LF82. Read More

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December 2018

Lipocalin-2 Functions as Inhibitor of Innate Resistance to .

Front Immunol 2018 26;9:2717. Epub 2018 Nov 26.

Granulocyte Research Laboratory, Rigshospitalet, Copenhagen, Denmark.

Lipocalin-2 is a constituent of the neutrophil secondary granules and is expressed by macrophages and epithelium in response to inflammation. Lipocalin-2 acts in a bacteriostatic fashion by binding iron-loaded siderophores required for bacterial growth. (M. Read More

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November 2018

Draft Genome Sequence of Mycobacterium parafortuitum Strain P7335.

Microbiol Resour Announc 2018 Aug 30;7(8). Epub 2018 Aug 30.

IRD MEPHI IHU Méditerranée Infection, Aix-Marseille University, Marseille, France.

Mycobacterium parafortuitum is a rapidly growing nontuberculous mycobacterium, initially isolated from soil in Japan. The 6,175,772-bp draft genome sequence of M. parafortuitum strain P7335 exhibits a G+C content of 68. Read More

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Complete Genome Sequences of Mycobacteriophages Kwksand96 and Cane17.

Microbiol Resour Announc 2018 Nov 1;7(17). Epub 2018 Nov 1.

Department of Biological and Environmental Sciences, University of West Alabama, Livingston, Alabama, USA.

Bacteriophages Kwksand96 and Cane17 were isolated from Mycobacterium smegmatis mc155. M. smegmatis is host to the highest number of phages analyzed from one species. Read More

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November 2018

Complete Genome Sequence of Cluster A1 Mycobacterium smegmatis Bacteriophage Arlo.

Microbiol Resour Announc 2018 Oct 25;7(16). Epub 2018 Oct 25.

Department of Biological Sciences, Tarleton State University, Stephenville, Texas, USA.

Mycobacteriophage Arlo is a newly isolated bacteriophage isolated from soil samples collected in Bluff Dale, Texas. Mycobacteriophage Arlo has a 52,960 base-pair double-stranded DNA genome that is predicted to contain 96 protein-coding genes. Mycobacteriophage Arlo is related to mycobacteriophage DD5 and other cluster A1 bacteriophages. Read More

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October 2018

Draft Genome Sequence of Mycobacterium shimoidei Strain P7336.

Microbiol Resour Announc 2018 Oct 4;7(13). Epub 2018 Oct 4.

Aix-Marseille Université, IRD, MEPHI, IHU Méditerranée Infection, Marseille, France.

Mycobacterium shimoidei, a slow-growing, nontuberculous mycobacterium initially isolated from a Japanese man, is a rare opportunistic lung pathogen. The 4,842,631-bp draft genome of M. shimoidei strain P7336 exhibits a 65. Read More

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October 2018

Draft Genome Sequences of Mycolicibacter senuensis Isolate GF74 and Mycobacterium colombiense Isolates GF28 and GF76 from a Swine Farm in Japan.

Microbiol Resour Announc 2018 Sep 13;7(10). Epub 2018 Sep 13.

Department of Microbiology, Graduate School of Medicine, Kyoto University, Sakyo-ku, Kyoto, Japan.

Several nontuberculous mycobacteria (NTM) occasionally infect humans and animals. Here, we report the draft genome sequences of Mycolicibacter senuensis isolate GF74 (4,792,997 bp) and Mycobacterium colombiense isolates GF28 and GF76 (5,473,554 bp and 5,426,852 bp, respectively) isolated from a swine farm in Japan. These sequences provide further information on NTM research. Read More

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September 2018

Long-lasting cutaneous tuberculosis owing to masquerading as sarcoidosis.

JAAD Case Rep 2019 Jan 27;5(1):1-4. Epub 2018 Nov 27.

Dermatology Department, University Hospital of Bordeaux, Bordeaux, France.

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January 2019

Mycobacterium tuberculosis Infection in the Setting of Interstitial Lung Disease: Coincidence or Bad Luck?

Cureus 2018 Oct 1;10(10):e3391. Epub 2018 Oct 1.

Pulmonology, New York Presbyterian Hospital Queens, New York, USA.

Mycobacterium tuberculosis (MTB) infection is the ninth leading cause of death worldwide, with many individuals with undiagnosed active or latent disease. The presence of parenchymal lung disease, such as interstitial lung disease (ILD), has been suggested to increase the risk of pulmonary tuberculosis (TB). In the clinical setting of ILD, the diagnosis of an underlying MTB infection may be challenging due to the interstitial process and underlying fibrosis, which may mask the infection. Read More

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October 2018

MtrA Response Regulator Controls Cell Division and Cell Wall Metabolism and Affects Susceptibility of Mycobacteria to the First Line Antituberculosis Drugs.

Front Microbiol 2018 23;9:2839. Epub 2018 Nov 23.

Biomedical Research, The University of Texas Health Science Center, Tyler, TX, United States.

The biological processes regulated by the essential response regulator MtrA and the growth conditions promoting its activation in , a slow grower and pathogen, are largely unknown. Here, using a gain-of-function mutant, MtrA, which functions in the absence of the cognate MtrB sensor kinase, we show that the MtrA regulon includes several genes involved in the processes of cell division and cell wall metabolism. The expression of selected MtrA targets and intracellular MtrA levels were compromised under replication arrest induced by genetic manipulation and under stress conditions caused by toxic radicals. Read More

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November 2018

Vitamin D3 as adjunctive therapy in the treatment of depression in tuberculosis patients: a short-term pilot randomized double-blind controlled study.

Neuropsychiatr Dis Treat 2018 14;14:3103-3109. Epub 2018 Nov 14.

Department of Tuberculosis Control, Ningbo Municipal Center for Disease Control & Prevention, Ningbo, Zhejiang, People's Republic of China,

Objective: We aimed to evaluate whether high-dose cholecalciferol has beneficial effects on depression in pulmonary tuberculosis (PTB) patients.

Methods: This pilot, randomized, and double-blind trial enrolled 123 recurrent PTB patients (aged ≥18 years) meeting criteria of major depressive disorder from four hospitals in Southeast China. Patients were randomly assigned to 8-week oral treatment with 100,000 IU/week cholecalciferol (Vit D group) or a matching placebo (control group). Read More

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November 2018

Mycobacterial glycolipid Di-O-acyl trehalose promotes a tolerogenic profile in dendritic cells.

PLoS One 2018 10;13(12):e0207202. Epub 2018 Dec 10.

Departamento de Inmunología, Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, México City, México.

Due to prolonged coevolution with the human being, Mycobacterium tuberculosis has acquired a sophisticated capacity to evade host immunity and persist in a latent state in the infected individual. As part of this evolutive process, mycobacteria have developed a highly complex cell wall that acts as a protective barrier. Herein we studied the effects of Di-O-acyl trehalose, a cell-wall glycolipid of virulent mycobacteria on murine bone marrow-derived dendritic cells. Read More

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December 2018

Nested Polymerase Chain Reaction and Cutaneous Tuberculosis.

Am J Dermatopathol 2018 Nov 27. Epub 2018 Nov 27.

Unidad de Investigación Médica en Inmunoquímica, Hospital de Especialidades, Centro Médico Nacional SXXI, IMSS, Mexico City, México.

The role of Mycobacterium tuberculosis in the etiology and pathogenesis of cutaneous tuberculosis is controversial because of the difficulties associated with demonstrating the presence of these mycobacteria in tuberculid cutaneous lesions by routinely available microbiological and histological techniques. In this study, we aimed to demonstrate the presence of M. tuberculosis in cutaneous tuberculosis. Read More

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November 2018

How to kill dormant .

Int J Mycobacteriol 2018 Oct-Dec;7(4):399-400

A.N. Bach Institute of Biochemistry, Federal Research Centre "Fundamentals of Biotechnology" of the Russian Academy of Sciences, Moscow, Russia.

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December 2018

Macrolides and mycobacterium abscessus abscessus: A good idea?

Int J Mycobacteriol 2018 Oct-Dec;7(4):394

Department of Paediatric Respiratory Medicine, Royal Hospital for Sick Children, Edinburgh, Scotland.

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December 2018

Unrecognized and delayed to diagnose of adolescence tuberculosis case in a boarding school in West Java, Indonesia.

Int J Mycobacteriol 2018 Oct-Dec;7(4):387-389

Department of Child Health, Faculty of Medicine, Universitas Padjadjaran, Bandung, West Java, Indonesia.

Adolescent is a high-risk population for developing tuberculosis (TB) disease, unfortunately adolescence TB remains to be neglected. Clinical manifestations of TB are usually chronic and nonspecific that could be mimicking other disease. Unrecognized TB disease will lead to misdiagnosis, delayed, and inappropriate management. Read More

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December 2018

Infection of multiple strains among tuberculosis/human immunodeficiency virus co-infected patients: A molecular study in Myanmar.

Int J Mycobacteriol 2018 Oct-Dec;7(4):375-379

Department of Epidemiology Unit, Faculty of Medicine, Prince of Songkla University, Hat Yai, Songkhla, Thailand.

Background: Appearance of Mycobacterium tuberculosis (MTB) in the sputum of a tuberculosis (TB)/human immunodeficiency virus (HIV) co-infected patient under treatment may indicate either failure or new infection. This study aims to evaluate whether TB treatment failure among TB/HIV co-infected patients is a real failure.

Methods: A prospective cohort study was conducted among 566 TB/HIV co-infected patients who started TB treatment in 12 townships in the upper Myanmar. Read More

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December 2018

Expression, purification, and characterization of pyruvate kinase from : A key allosteric regulatory enzyme.

Int J Mycobacteriol 2018 Oct-Dec;7(4):368-374

Department of Biotechnology, Kuvempu University, Shimoga, Karnataka, India.

Background: The current research aims to isolate pyruvate kinase (Pyk) gene from Mycobacterium tuberculosis and expression of the gene (Rv1617) to obtain a purified enzyme. The enzyme activity and secondary structural features were assessed through biochemical assays and circular dichroism (CD) spectroscopy, respectively.

Methods: The Pyk-encoding gene from the complete genome of M. Read More

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December 2018

Antimycobacterial activity of nonantibiotics associated with the polypharmacy of cystic fibrosis (CF) against mycobacterium abscessus.

Int J Mycobacteriol 2018 Oct-Dec;7(4):358-360

Centre for Experimental Medicine, Queen's University; Department of Bacteriology, Northern Ireland Public Health Laboratory, Belfast City Hospital, Northern Ireland, UK.

Background: Antimicrobial resistance (AMR) has rendered certain species of Mycobacterium difficult to treat clinically, particularly the nontuberculous Mycobacterium, Mycobacterium abscessus, in patients with cystic fibrosis (CF). Such patients are treated with several nonantibiotic medicines, which may have antimicrobial properties. Given the growing burden of AMR in M. Read More

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December 2018

The association between computed tomography scan findings of pulmonary infection caused by atypical mycobacteria and bacillus count in sputum samples.

Int J Mycobacteriol 2018 Oct-Dec;7(4):355-357

Pediatric Respiratory Diseases Research Center, National Research Institute of Tuberculosis and Lung Disease, Shahid Beheshti University of Medical Sciences, Tehran, Iran.

Background: The use of imaging techniques is important for prompt diagnosis and treatment of atypical mycobacterial infections; it also reduces the burden of these infections. The purpose of the present study was to determine the association between computed tomography (CT) scan findings of pulmonary infection caused by atypical mycobacteria and bacillus count in sputum samples.

Methods: This cross-sectional, observational, comparative study included 50 consecutive patients with pulmonary infection caused by atypical mycobacteria, who were hospitalized in Masih-Daneshvari Hospital of Tehran, Iran during 2012-2017. Read More

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December 2018

TMC 5135 as a vaccine candidate against tuberculosis: studies.

Int J Mycobacteriol 2018 Oct-Dec;7(4):343-346

Laboratories of Biological-Pharmaceutical Productions (LABIOFAM), Havana, Cuba.

Background: Tuberculosis (TB) is one of the biggest problems of global health, at present. Bacillus-Calmette-Guérin is the only vaccine available against this disease. It protects only against the severe forms of TB in the childhood, which is a challenge in the search of new vaccine candidates. Read More

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December 2018

Mitigating polymerase chain reaction/amplicon contamination in a high-risk high-burden mycobacterial reference laboratory in a resource-limited setting.

Int J Mycobacteriol 2018 Oct-Dec;7(4):332-337

Intermediate Reference Laboratory, State TB Demonstration cum Training Centre, Kolkata, West Bengal, India.

Background: Nucleic acid amplification techniques have become important machineries in the diagnosis of several diseases in clinical laboratories. Polymerase chain reaction (PCR) contamination/amplicon contamination leading to false positivity remains a major concern in these laboratories. Prevention of these contaminations in establishing these molecular biology laboratories has been very crucial over the years. Read More

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December 2018

Nitrate and nitrite reductase activities of .

Int J Mycobacteriol 2018 Oct-Dec;7(4):328-331

Department of Biological Sciences, Virginia Tech, Blacksburg, Virginia, USA.

Background: In spite of the fact that the standard test for nitrate reductase activity is negative for Mycobacterium avium, it can grow in a defined minimal medium with either nitrate (NO) or nitrite (NO) as sole nitrogen sources.

Methods: NO-and NO-reductase activities were measured in soluble and membrane fractions of aerobically grown cells of M. avium and those grown aerobically and shifted to anaerobiosis. Read More

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December 2018

Crystal violet decolorization assay for rapid detection of multidrug-resistant isolates: A multicenter study.

Int J Mycobacteriol 2018 Oct-Dec;7(4):310-314

Mycobacteria Service, INEI ANLIS "Dr. Carlos G. Malbran," Buenos Aires, Argentina.

Background: Effective control of tuberculosis is achieved by early diagnosis and drug susceptibility testing for initiation of appropriate treatment. The performance of crystal violet decolorization assay (CVDA) for susceptibility testing of Mycobacterium tuberculosis to isoniazid (INH) and rifampicin (RIF) was compared in a multicenter study.

Methods: Seventy-two M. Read More

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December 2018

Inhibitors of energy metabolism interfere with antibiotic-induced death in mycobacteria.

J Biol Chem 2018 Dec 7. Epub 2018 Dec 7.

Lee Kong Chian School of Medicine and School of Biological Sciences, Singapore.

Energy metabolism has recently gained interest as a target space for antibiotic drug development in mycobacteria. Of particular importance is bedaquiline (Sirturo), which kills mycobacteria by inhibiting the FF ATP synthase. Other components of the electron transport chain such as the NADH dehydrogenases (NDH-2 and NdhA) and the terminal respiratory oxidase are also susceptible to chemical inhibition. Read More

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December 2018

Discovery of 16-demethylrifamycins by Removing Predominant Polyketide Biosynthetic Pathway in sp. TP-A0468.

Appl Environ Microbiol 2018 Dec 7. Epub 2018 Dec 7.

State Key Laboratory of Bio-organic and Natural Products Chemistry, Shanghai Institute of Organic Chemistry, Chinese Academy of Sciences, 345 Lingling Road, Shanghai 200032, China

A number of strategies have been developed to mine novel natural products based on biosynthetic gene clusters and there has been dozens of successful cases facilitated by the development of genomic sequencing. During our study on biosynthesis of the antitumor polyketide kosinostatin (KST), we found that the genome of sp. TP-A0468, the producer of KST, contains other potential polyketide gene clusters, with no encoded products detected. Read More

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December 2018

Molecular modelling and competitive inhibition of a Mycobacterium tuberculosis multidrug-resistance efflux pump.

J Mol Graph Model 2018 Dec 3;87:98-108. Epub 2018 Dec 3.

Laboratory of Computational Biology, Computational Sciences Center of the Universidade Federal do Rio Grande, Avenida Itlia, Km8, Rio Grande, RS, Brazil.

Tuberculosis is a major cause of mortality and morbidity in developing countries, and the emergency of multidrug and extensive drug resistance cases is an utmost issue on the control of the disease. Despite the efforts on the development of new antibiotics, eventually there will be strains resistant to them as well. Efflux plays an important role in the evolution of resistance in Mycobacterium tuberculosis. Read More

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December 2018

Early ancient sublineages of Mycobacterium tuberculosis Beijing genotype: unexpected clues from phylogenomics of the pathogen and human history.

Clin Microbiol Infect 2018 Dec 5. Epub 2018 Dec 5.

Department of Epidemiology, Omsk State Medical University, Omsk, Russia.

Objective: The Mycobacterium tuberculosis Beijing family is an epidemiologically important lineage subdivided into large-scale phylogenetic sublineages: ancient, endemic in East Asia, and global modern. Here, we analyzed ancient sublineages of the Beijing genotype in the Omsk region of southwestern Siberia, an intriguing area at the intersection of European Russia, Siberia, and Central Asia.

Methods: The study included 423 M. Read More

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December 2018

Differential T cell responses against DosR-associated antigen Rv2028c in BCG-vaccinated populations with tuberculosis infection.

J Infect 2018 Dec 5. Epub 2018 Dec 5.

Shanghai Public Health Clinical Center, Key Laboratory of Medical Molecular Virology of MOE/MOH, Fudan University, 2901 Caolang Rd., Shanghai 201508, China; School of Laboratory Medicine and Life Science, Wenzhou Medical University, Wenzhou 325035, China; School of Laboratory Medicine and Life Science, Jinlin Agriculture University, Changchun 130033, China. Electronic address:

The IFN-γ release assays (IGRAs) based on region of difference 1 (RD1) antigens have improved diagnosis of Mycobacterium tuberculosis (Mtb) infection. However, IGRAs with these antigens could not distinguish latent tuberculosis infection (LTBI) from active tuberculosis (ATB). DosR regulon genes are thought to be important for Mtb dormancy, and their products have higher immunogenicity in LTBI than ATB individuals, suggesting protective immunity mediated by DosR regulon-encoded antigens and potential utility of them for differential diagnostics of Mtb-infected populations or development of therapeutic vaccines against tuberculosis (TB). Read More

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December 2018

Diagnostic performance of plasma cytokine biosignature combination and MCP-1 as individual biomarkers for differentiating among Mycobacterium tuberculosis infection.

J Infect 2018 Dec 5. Epub 2018 Dec 5.

Department of Clinical Laboratory, Institute of Surgery Research, Daping Hospital, Army Medical University (Third Military Medical University), Chongqing, 400042, China. Electronic address:

Objective: We aimed to identify plasma cytokine biomarkers that differentiate the infection stages of Mycobacterium tuberculosis (MTB).

Methods: This study included a total of 227 subjects consisting of active tuberculosis (ATB) patients, latent tuberculosis infection (LTBI) individuals, and healthy controls (HC). We analyzed the expressions of 38 plasma cytokines in the discovery cohort to identify the biosignatures for differentiating MTB infection states, area under the curve (AUC) were used to evaluate the diagnostic efficiency. Read More

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December 2018
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Assessing and screening for T-cell epitopes from Mycobacterium tuberculosis RD2 proteins for the diagnosis of active tuberculosis.

Braz J Infect Dis 2018 Dec 5. Epub 2018 Dec 5.

Fudan University, Huashan Hospital, Department of Infectious Diseases, Shanghai, China. Electronic address:

The Region of D eletion 2 (RD2) of Mycobacterium tuberculosis encodes reserved antigens that contribute to bacterial virulence. Among these antigens, Rv1983, Rv1986, Rv1987, and Rv1989c have been shown to be immunodominant in infected cattle; however, their diagnostic utility has not been evaluated in humans. In this study, we screened 87 overlapping synthetic peptides encoded by five RD2 proteins for diagnosing tuberculosis epitopes in 50 active tuberculosis (TB) cases, 31 non-tuberculosis patients and 36 healthy individuals. Read More

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December 2018

An assessment of the persistence of pathogenic bacteria removal in chicken manure compost employing clay as additive via meta-genomic analysis.

J Hazard Mater 2018 Nov 30;366:184-191. Epub 2018 Nov 30.

College of Natural Resources and Environment, Northwest A&F University, Yangling, Shaanxi Province, 712100, China. Electronic address:

The aim was to evaluate pathogenic bacteria (PB) survival during the composting of chicken manure (CM) amended with five different dosages of clay compared to CM without clay-applied treatment. The results showed that 85-87% of PB relative abundances (RAs) were significantly reduced in lower dosages of applied clay (T2 and T3). However, the maximum survival of PB was noticed in the T6 and T5 treatments, but most of the PB belong to Firmicutes, Actinobacteria and Proteobacteria phylum and their derivative bacterial species. Read More

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November 2018

Evaluation of the culture-enhanced Xpert MTB/RIF assay for the diagnosis of smear-negative tuberculosis.

Med Mal Infect 2018 Dec 6. Epub 2018 Dec 6.

Service de microbiologie, hôpital Beaujon, AP-HP, Clichy, France; Faculté de médecine D. Diderot, université Paris VII, Paris, France; Inserm U 773, centre de recherche biomédicale Bichat-Beaujon, CRB3, Paris, France.

Objectives: To evaluate a new tool for the early diagnosis of tuberculosis.

Methods: A total of 374 smear-negative clinical specimens from patients with suspected tuberculosis were evaluated using a new procedure consisting of a preliminary step of culture in broth bottles followed by the detection of Mycobacterium tuberculosis complex (Mtb) and rifampicin resistance by the Xpert MTB/RIF assay (XMTB-RIF).

Results: A total of 30 Mtb strains were isolated, all susceptible to rifampicin. Read More

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December 2018

A method for improved fluorescent staining for acid fast smear microscopy by incorporating an acetone rinse step.

Diagn Microbiol Infect Dis 2018 Nov 9. Epub 2018 Nov 9.

Public Health Ontario Laboratory, Public Health Ontario, Toronto, Canada; Department of Laboratory Medicine and Pathobiology, Faculty of Medicine, University of Toronto, Canada. Electronic address:

Microscopic examination of the specimen smear for acid fast bacilli (AFB) provides a simple and rapid means of detecting AFB using fluorescent stain methods and remains a valuable diagnostic test used worldwide to identify and manage suspect cases of tuberculosis (TB). Methods to improve AFB smear staining protocols could provide better detection of suspect TB cases. In particular, decreasing background debris may improve the detection of smears with low numbers of bacilli. Read More

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November 2018

Mannosylated structures of mycobacterial lipoarabinomannans facilitate the maturation and activation of dendritic cells.

Cell Immunol 2018 Nov 30. Epub 2018 Nov 30.

Shanghai Public Health Clinical Center, Shanghai, China; Department of Immunology and Microbiology, Shanghai Jiao Tong University School of Medicine, Shanghai, China. Electronic address:

Lipoarabinomannan (LAM) is an important virulent factor secreted by mycobacteria, which generally elicit a strong immune response in the host. In this study, the structural difference of LAMs from three mycobacterial strains, Mycobacterium tuberculosis H37Rv, Mycobacterium smegmatis mc155 and a newly discovered clinical isolate, M. sp. Read More

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November 2018

Benzoylthioureas: Design, Synthesis and Antimycobacterial Evaluation.

Med Chem 2018 Dec 7. Epub 2018 Dec 7.

Departamento de Química, Centro de Ciências Exatas, Universidade Estadual de Londrina, Rodovia Celso Garcia Cid (PR 445), Km 380, Campus Universitário, Londrina - PR, 86057-970. Brazil.

Background: New drugs and strategies to treat tuberculosis (TB) are urgently needed. In this context, thiourea derivatives have a wide range of biological activities, including anti-TB. This fact can be illustrated with the structure of isoxyl, an old anti-TB drug, which have a thiourea as pharmacophore group. Read More

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December 2018

Pulmonary Delivery of Isoniazid in Nanogel-Loaded Chitosan Hybrid Microparticles for Inhalation.

J Aerosol Med Pulm Drug Deliv 2018 Dec 8. Epub 2018 Dec 8.

1 Department of Pharmaceutics, Faculty of Pharmacy, Helwan University , Helwan, Egypt .

Purpose: Inhalable pulmonary delivery of isoniazid (INH) may improve the efficacy and reduce drug-resistant problem.

Method: INH-loaded chitosan microparticles (Cs-Mps-1-3) were prepared as inhalable carrier for the previously prepared INH-loaded polyvinylpyrrolidone/polyitaconic acid nanoparticles (NPs) using spray-drying technique. Here, Cs-Mps-1-3 are composed of Cs: INH-loaded NPs: Free INH at w/w ratios (1:1:0), (1: 0:1), and (1:1:1), respectively. Read More

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December 2018

Differentiation between persistent infection/colonization and re-infection/re-colonization of Mycobacterium abscessus isolated from patients in Northeast Thailand.

Infect Genet Evol 2018 Dec 3;68:35-42. Epub 2018 Dec 3.

Department of Microbiology, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand; Research and Diagnostic Center for Emerging Infectious Diseases, Khon Kaen University, Khon Kaen, Thailand. Electronic address:

Mycobacterium abscessus can cause true infection or be present in the host as a harmless colonist. The ability of M. abscessus to cause disease and develop drug resistance is known to have a genetic basis. Read More

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December 2018
3 Reads

A Xenopus tadpole alternative model to study innate-like T cell-mediated anti-mycobacterial immunity.

Dev Comp Immunol 2018 Dec 3. Epub 2018 Dec 3.

Department of Microbiology and Immunology, University of Rochester Medical Center, United States. Electronic address:

Owing to the high incidence of multi-drug resistance and challenges posed by the complex and long duration of treatments, Mycobacterium tuberculosis (Mtb) infections remain a significant clinical burden, which would benefit from development of novel immuno-therapeutic-based treatment strategies. Among early immune effectors, invariant or innate-like (i)T cells are attracting attention because of their potential regulatory activity, which can shape anti-mycobacterial immune responses. Unlike conventional T cells, iT cells express a semi-invariant T cell receptor, and respond rapidly and robustly to molecular patterns presented by MHC class I-like molecules. Read More

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December 2018
3 Reads

Identification of risk factors and diagnostic methodology in serositis by M. tuberculosis

Rev Med Inst Mex Seguro Soc 2018 11 30;56(4):364-370. Epub 2018 Nov 30.

Secretaría de Salud, Hospital General de México “Dr. Eduardo Liceaga”, Unidad de Neumología y Cirugía de Tórax. Ciudad de México, México

Background: Tuberculosis is a public health problem, extrapulmonary presentations have increased, it is difficult to diagnose because of the low bacillary load.

Objective: To identify risk factors and to evaluate the efficiency of diagnostic methods in pleural, meningeal, peritoneal and pericardial tuberculosis.

Methods: Prospective study of cases and controls. Read More

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November 2018

Interleukin-32ε induces caspase-independent apoptosis mediated by N-Myc interactor in macrophages infected with Mycobacterium tuberculosis.

FEBS J 2018 Dec 6. Epub 2018 Dec 6.

Key Laboratory of Animal Biotechnology of the Ministry of Agriculture, College of Veterinary Medicine, Northwest A&F University, Yangling, 712100, Shaanxi, China.

Mycobacterium tuberculosis (Mtb) is the pathogen responsible for tuberculosis, a leading cause of illness and death worldwide. Growing evidence suggests that the proinflammatory cytokine IL-32 plays a major role in host defenses against pathogens such as Mtb. IL-32 exists in six alternatively spliced isoforms, but anti-tuberculosis effects have been reported only for some of them. Read More

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December 2018

BCG Vaccination for bovine tuberculosis; conclusions from the jersusalem one health workshop.

Transbound Emerg Dis 2018 Dec 6. Epub 2018 Dec 6.

Department of Microbiology and Molecular Genetics, IMRIC Hebrew University of Jerusalem, Jerusalem, Israel.

The global burden of bovine tuberculosis (bTB) remains poorly characterized, with spill-over impacts on multiple species. The "One Health" concept is especially relevant given the bidirectional risk of cattle infecting humans with Mycobacterium bovis and humans infecting cattle with M. tuberculosis. Read More

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December 2018
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Target discovery for new antitubercular drugs using a large dataset of growth inhibitors from PubChem.

Authors:
Robert C Goldman

Infect Disord Drug Targets 2018 Dec 5. Epub 2018 Dec 5.

Drug Discovery and Development, RCG Consulting, 16024 Golden Lakes Drive. United States.

The number of drugs available for treatment of active tuberculosis are diminishing due to increased multidrug resistance selection in Mycobacterium tuberculosis leading to multiple (MDR) and extensively (XDR) resistant strains. Also, TB is treated with multiple drugs to minimize further resistance development, mandating a sustained effort to identify new lead compounds for treating drug resistant TB, and shortening time to cure for all TB infections. High throughput screening, a well-known approach to discovery of new leads, is conducted in two basic modes 1) using whole cells and screening for inhibition of growth, or whole cell reporter cells that signals when a specific pathway is perturbed, and 2) in vitro non-cell based enzyme or other functional assays for direct ligand-target binding. Read More

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December 2018