109,650 results match your criteria Mycobacterium Avium-Intracellulare


Differentiation between persistent infection/colonization and re-infection/re-colonization of Mycobacterium abscessus isolated from patients in Northeast Thailand.

Infect Genet Evol 2018 Dec 3. Epub 2018 Dec 3.

Department of Microbiology, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand; Research and Diagnostic Center for Emerging Infectious Diseases, Khon Kaen University, Khon Kaen, Thailand. Electronic address:

Mycobacterium abscessus can cause true infection or be present in the host as a harmless colonist. The ability of M. abscessus to cause disease and develop drug resistance is known to have a genetic basis. Read More

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December 2018
2 Reads

A Xenopus tadpole alternative model to study innate-like T cell-mediated anti-mycobacterial immunity.

Dev Comp Immunol 2018 Dec 3. Epub 2018 Dec 3.

Department of Microbiology and Immunology, University of Rochester Medical Center, United States. Electronic address:

Owing to the high incidence of multi-drug resistance and challenges posed by the complex and long duration of treatments, Mycobacterium tuberculosis (Mtb) infections remain a significant clinical burden, which would benefit from development of novel immuno-therapeutic-based treatment strategies. Among early immune effectors, invariant or innate-like (i)T cells are attracting attention because of their potential regulatory activity, which can shape anti-mycobacterial immune responses. Unlike conventional T cells, iT cells express a semi-invariant T cell receptor, and respond rapidly and robustly to molecular patterns presented by MHC class I-like molecules. Read More

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December 2018

Identification of risk factors and diagnostic methodology in serositis by M. tuberculosis

Rev Med Inst Mex Seguro Soc 2018 11 30;56(4):364-370. Epub 2018 Nov 30.

Secretaría de Salud, Hospital General de México “Dr. Eduardo Liceaga”, Unidad de Neumología y Cirugía de Tórax. Ciudad de México, México

Background: Tuberculosis is a public health problem, extrapulmonary presentations have increased, it is difficult to diagnose because of the low bacillary load.

Objective: To identify risk factors and to evaluate the efficiency of diagnostic methods in pleural, meningeal, peritoneal and pericardial tuberculosis.

Methods: Prospective study of cases and controls. Read More

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November 2018

Interleukin-32ε induces caspase-independent apoptosis mediated by N-Myc interactor in macrophages infected with Mycobacterium tuberculosis.

FEBS J 2018 Dec 6. Epub 2018 Dec 6.

Key Laboratory of Animal Biotechnology of the Ministry of Agriculture, College of Veterinary Medicine, Northwest A&F University, Yangling, 712100, Shaanxi, China.

Mycobacterium tuberculosis (Mtb) is the pathogen responsible for tuberculosis, a leading cause of illness and death worldwide. Growing evidence suggests that the proinflammatory cytokine IL-32 plays a major role in host defenses against pathogens such as Mtb. IL-32 exists in six alternatively spliced isoforms, but anti-tuberculosis effects have been reported only for some of them. Read More

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December 2018

BCG Vaccination for bovine tuberculosis; conclusions from the jersusalem one health workshop.

Transbound Emerg Dis 2018 Dec 6. Epub 2018 Dec 6.

Department of Microbiology and Molecular Genetics, IMRIC Hebrew University of Jerusalem, Jerusalem, Israel.

The global burden of bovine tuberculosis (bTB) remains poorly characterized, with spill-over impacts on multiple species. The "One Health" concept is especially relevant given the bidirectional risk of cattle infecting humans with Mycobacterium bovis and humans infecting cattle with M. tuberculosis. Read More

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December 2018
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Target discovery for new antitubercular drugs using a large dataset of growth inhibitors from PubChem.

Authors:
Robert C Goldman

Infect Disord Drug Targets 2018 Dec 5. Epub 2018 Dec 5.

Drug Discovery and Development, RCG Consulting, 16024 Golden Lakes Drive. United States.

The number of drugs available for treatment of active tuberculosis are diminishing due to increased multidrug resistance selection in Mycobacterium tuberculosis leading to multiple (MDR) and extensively (XDR) resistant strains. Also, TB is treated with multiple drugs to minimize further resistance development, mandating a sustained effort to identify new lead compounds for treating drug resistant TB, and shortening time to cure for all TB infections. High throughput screening, a well-known approach to discovery of new leads, is conducted in two basic modes 1) using whole cells and screening for inhibition of growth, or whole cell reporter cells that signals when a specific pathway is perturbed, and 2) in vitro non-cell based enzyme or other functional assays for direct ligand-target binding. Read More

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December 2018

Predicting Drug Interactions From Chemogenomics Using INDIGO.

Methods Mol Biol 2019 ;1888:219-231

Department of Biomedical Engineering, University of Michigan, Ann Arbor, MI, USA.

Designing effective antibiotic combination regimens is critical for countering drug resistance in pathogens. Yet the large combinatorial search-space makes the identification of effective combinations a significant challenge. There is a great need for computational approaches that can rapidly prioritize potential combination regimens based on the antagonistic and synergistic interactions among the constituent antibiotics. Read More

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January 2019

Endophytic Actinomycetes from Tea Plants (): Isolation, Abundance, Antimicrobial, and Plant-Growth-Promoting Activities.

Biomed Res Int 2018 1;2018:1470305. Epub 2018 Nov 1.

FAFU-UCR Joint Center for Horticultural Biology and Metabolomics, Fujian Provincial Key Laboratory of Haixia Applied Plant Systems Biology, Fujian Agriculture and Forestry University, Fuzhou 350002, China.

Endophytic actinomycetes are a promising source of novel metabolites with diverse biological activities. Tea plants () produce arsenals of phytochemicals, which are linked to a number of medicinal and nutritional properties. However, a systematic investigation into the abundance and diversity of cultivated actinomycetes residing in tea plants has not been performed. Read More

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November 2018
1 Read

Surgery in nontuberculous mycobacteria pulmonary disease.

Breathe (Sheff) 2018 Dec;14(4):288-301

Discipline of Child and Adolescent Health, Sydney Medical School, University of Sydney, Sydney, Australia.

Medical treatment of pulmonary nontuberculous mycobacteria (NTM) disease has highly variable outcomes. Despite the use of multiple antibiotics, sputum clearance is often difficult to achieve, especially in cases with macrolide resistant NTM infection. Immunocompromised patients and those with structural lung disease are at increased risk, although occurrence in immunocompetent patients without structural lung disease is well recognised. Read More

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December 2018
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The inflammasome in leprosy skin lesions: an immunohistochemical evaluation.

Infect Drug Resist 2018 12;11:2231-2240. Epub 2018 Nov 12.

Center of Biological and Health Science, State University of Para, Belem, Brazil,

Objective: Leprosy is a chronic infectious disease presenting with a spectrum of clinical manifestations that correspond to the type of immune response that develops in the host. Factors that may be involved in this process include inflammasomes, cytosolic proteins responsible for the activation of caspase 1, IL-1β and IL-18 secretion, and induction of a type of death called pyroptosis.

Patients And Methods: We evaluated the expression of inflammasome markers (nucleotide-binding oligomerization domain-like receptor containing pyrin domain 1 [NLRP1], nucleotide-binding oligomerization domain-like receptor containing pyrin domain 3 [NLRP3], caspase 1, IL-1β, and IL-18) by immunohistochemistry in 43 samples of skin lesions of leprosy patients from the groups indeterminate (I) leprosy (13 patients), tuberculoid (TT) leprosy (15 patients), and lepromatous leprosy (LL; 15 patients). Read More

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November 2018

Drug-resistance profiling and transmission dynamics of multidrug-resistant in Saudi Arabia revealed by whole genome sequencing.

Infect Drug Resist 2018 12;11:2219-2229. Epub 2018 Nov 12.

Mycobacteriology Research Section, Department of Infection and Immunity, King Faisal Specialist Hospital and Research Center, Riyadh 11211, Saudi Arabia,

Background: In Saudi Arabia, cross-border transmission of multidrug-resistant (MDR) complex (MTBC) strains might be particularly fostered by high immigration rates. Herein, we aimed to elucidate the transmission dynamics of MDR-MTBC strains and reveal a detailed prediction of all resistance-conferring mutations for the first- and second-line drugs.

Methods: We investigated all MDR-MTBC strains collected between 2015 and 2017 from provincial mycobacteria referral laboratories and compared demographic and clinical parameters to a cohort of non-MDR-TB patients using a whole genome sequencing approach. Read More

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November 2018

Structure of a functional obligate complex IIIIV respiratory supercomplex from Mycobacterium smegmatis.

Nat Struct Mol Biol 2018 Dec 5. Epub 2018 Dec 5.

Department of Biochemistry and Biophysics, Arrhenius Laboratories for Natural Sciences, Stockholm University, Stockholm, Sweden.

In the mycobacterial electron-transport chain, respiratory complex III passes electrons from menaquinol to complex IV, which in turn reduces oxygen, the terminal acceptor. Electron transfer is coupled to transmembrane proton translocation, thus establishing the electrochemical proton gradient that drives ATP synthesis. We isolated, biochemically characterized, and determined the structure of the obligate IIIIV supercomplex from Mycobacterium smegmatis, a model for Mycobacterium tuberculosis. Read More

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December 2018
2 Reads

Ion mobility-mass spectrometry analysis of diarylquinoline diastereomers: Drugs used for tuberculosis treatment.

Eur J Mass Spectrom (Chichester) 2018 Dec 5:1469066718813226. Epub 2018 Dec 5.

1 Normandie Université, COBRA, UMR 6014 et FR 3038; Université de Rouen; INSA de Rouen; CNRS, IRCOF, Mont Saint Aignan Cedex, France.

Mycobacterium tuberculosis infection results in more than two million deaths per year and is the leading cause of mortality in people infected with HIV. A new structural class of antimycobacterials, the diarylquinolines, has been synthesized and is being highly effective against both M. tuberculosis and multidrug-resistant tuberculosis. Read More

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December 2018

A Systematic Overview of Type II and III Toxin-Antitoxin Systems with a Focus on Druggability.

Toxins (Basel) 2018 Dec 4;10(12). Epub 2018 Dec 4.

The Research Institute of Pharmaceutical Sciences, College of Pharmacy, Seoul National University, Gwanak-gu, Seoul 08826, Korea.

Toxin-antitoxin (TA) systems are known to play various roles in physiological processes, such as gene regulation, growth arrest and survival, in bacteria exposed to environmental stress. Type II TA systems comprise natural complexes consisting of protein toxins and antitoxins. Each toxin and antitoxin participates in distinct regulatory mechanisms depending on the type of TA system. Read More

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December 2018

Advances in the molecular diagnosis of tuberculosis: From probes to genomes.

Infect Genet Evol 2018 Nov 30. Epub 2018 Nov 30.

Global Health and Tropical Medicine, GHTM, Instituto de Higiene e Medicina Tropical, IHMT, Universidade Nova de Lisboa, UNL, Lisboa, Portugal. Electronic address:

Tuberculosis, disease caused by Mycobacterium tuberculosis, is currently the leading cause of death by a single infectious agent worldwide. Early, rapid and accurate identification of M. tuberculosis and the determination of drug susceptibility is essential for the treatment and management of this disease. Read More

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November 2018

Design and synthesis of novel pyrimidine derivatives as potent antitubercular agents.

Eur J Med Chem 2018 Nov 26;163:169-182. Epub 2018 Nov 26.

State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, West China Medical School, Sichuan University, and Collaborative Innovation Center for Biotherapy, Chengdu, 610041, China. Electronic address:

The emergence of various drug-resistant Mycobacterium tuberculosis (Mtb) strains has necessitated the exploration of new drugs that lack cross-resistance with existing therapeutics. By screening the MedChemExpress bioactive compound library, ceritinib was identified as a compound with activity against Mtb H37Ra. Ceritinib had a MIC value of 9. Read More

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November 2018

Wavelet-domain elastic net for clustering on genomes strains.

Genet Mol Biol 2018 Nov 29. Epub 2018 Nov 29.

Empresa Brasileira de Pesquisa Agropecuária (Embrapa) Pecuária Sul. Bagé, RS, Brazil.

We propose to evaluate genome similarity by combining discrete non-decimated wavelet transform (NDWT) and elastic net. The wavelets represent a signal with levels of detail, that is, hidden components are detected by means of the decomposition of this signal, where each level provides a different characteristic. The main feature of the elastic net is the grouping of correlated variables where the number of predictors is greater than the number of observations. Read More

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November 2018

Identifying dormant growth state of mycobacteria by orthogonal analytical approaches on a single cell and ensemble basis.

Anal Chem 2018 Dec 3. Epub 2018 Dec 3.

Tuberculosis is currently the single most deadly infectious disease in the world and a public health priority as defined by WHO. Although the disease is in general curable, treatment success is hampered by the necessity of a long and side effect prone treatment. Low treatment efficiency may be partly due to the special growth states mycobacteria enter to avoid being killed by antibiotics and to persist longer within the host. Read More

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December 2018

HIV, HCMV and mycobacterial antibody levels: a cross-sectional study in a rural Ugandan cohort.

Trop Med Int Health 2018 Dec 1. Epub 2018 Dec 1.

Faculty of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, UK.

Objectives: A growing evidence base implicates human cytomegalovirus (HCMV) as a risk factor for TB disease. We investigated total IgG and mycobacteria-specific antibodies in a cross-sectional study nested within a rural Ugandan General Population Cohort (GPC), in relation to HIV infection and the magnitude of HCMV IgG response.

Methods: Sera from 2,189 individuals (including 27 sputum-positive TB cases) were analysed for antibodies against mycobacteria (Ag85A, PPD, LAM, ESAT6/CFP10) and HCMV, tetanus toxoid (TT) and total IgG. Read More

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December 2018

Association between CD53 genetic polymorphisms and tuberculosis cases.

Genes Genomics 2018 Nov 30. Epub 2018 Nov 30.

Department of Biomedical Laboratory Science, College of Life and Health Sciences, Hoseo University, Asan, Chungnam, 31499, South Korea.

Background: Tetraspanin proteins are expressed in various immune cells, and they play an important role in tuberculosis formation. CD53 is a protein in the tetraspanin family that is expressed in many white blood cells. In particular, it plays an important role in cytokine regulation and interaction between natural killer (NK) cells and antigen-presenting cells (APCs). Read More

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November 2018

Triatomines: Trypanosomatids, Bacteria, and Viruses Potential Vectors?

Front Cell Infect Microbiol 2018 16;8:405. Epub 2018 Nov 16.

Laboratório de Interação Patógeno-Hospedeiro, Instituto de Ciências Biológicas, Departamento de Biologia Celular, Universidade de Brasília, Brasília, Brazil.

Triatominae bugs are the vectors of Chagas disease, a major concern to public health especially in Latin America, where vector-borne Chagas disease has undergone resurgence due mainly to diminished triatomine control in many endemic municipalities. Although the majority of Triatominae species occurs in the Americas, species belonging to the genus occur in India, and species belonging to the complex have been also identified in Africa, the Middle East, South-East Asia, and in the Western Pacific. Not all of Triatominae species have been found to be infected with , but the possibility of establishing vector transmission to areas where Chagas disease was previously non-endemic has increased with global population mobility. Read More

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November 2018
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Identifying lung cancer in patients with active pulmonary tuberculosis.

J Thorac Dis 2018 Oct;10(Suppl 28):S3392-S3397

Division of Thoracic Surgery, Department of Surgery, Boston University School of Medicine, Boston, USA.

The diagnosis of lung cancer can be delayed in patients with a history of infection with pulmonary tuberculosis that present with new lesions on chest imaging, due to a high initial index of suspicion for mycobacterium tuberculosis complex rather than malignancy. This may lead to diagnosis of malignancy at a more advanced stage of the disease with subsequent increased morbidity and mortality. We reviewed the current literature to evaluate various methods of differentiating between a diagnosis of lung cancer and tuberculosis including radiography, computerized tomography (CT), positron emission tomography (PET) and various biological markers. Read More

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October 2018

First report of hepatobiliary infection developing obstructive jaundice in a patient with neutralizing anti-interferon-gamma autoantibodies.

New Microbes New Infect 2019 Jan 19;27:4-6. Epub 2018 Oct 19.

Keio University School of Medicine, Tokyo, Japan.

This study describes a patient who experienced hepatobiliary infection associated with neutralizing anti-interferon gamma (IFN-γ) autoantibodies during treatment for disseminated disease. Hepatobiliary infection should be considered in jaundiced patients with neutralizing anti-IFN-γ autoantibodies, including those receiving antimycobacterial therapy for disseminated disease. Read More

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January 2019

Human Immunodeficiency Virus and Tuberculosis Coinfection in a Tertiary Hospital in Southern Brazil: Clinical Profile and Outcomes.

Microbiol Insights 2018 27;11:1178636118813367. Epub 2018 Nov 27.

Universidade Positivo Medical School, Curitiba, Brazil.

Worldwide, the convergence of tuberculosis (TB) and human immunodeficiency virus type 1 (HIV-1) infection epidemics is a public health challenge. In Brazil, TB is the leading cause of death by infectious disease in people living with HIV (PLWH). This study aimed to report the clinical, demographic, epidemiological, and laboratory data for TB in PLWH. Read More

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November 2018

Clinico-Histological Correlation in Hansen's Disease: Three-year Experience at a Newly Established Tertiary Care Center in Central India.

Indian J Dermatol 2018 Nov-Dec;63(6):465-468

Department of Pathology and Lab Medicine, AIIMS, Bhopal, Madhya Pradesh, India.

Background: Hansen's disease is a chronic infectious disease caused by . It is characterized by a wide range of clinical and histological manifestations. Ridley-Jopling criteria are widely used for classifying leprosy. Read More

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December 2018

Genomic Reduction at TTC Repeats in the Bacterial Genome of Treated Cases of Hansen's Disease: A Possible Survival Mechanism of .

Indian J Dermatol 2018 Nov-Dec;63(6):449-454

Department of Biochemistry, IPGMER, Kolkata, West Bengal, India.

Introduction: has a small genome and a tendency of persisting as a very low-grade infection. The authors have shown earlier, that the changes in TTC repeats, in genome may contribute to the restriction of the pathogenicity of the bacterium and its survival strategy in case of pure neural Hansen's disease. We suspect, that a similar genomic reduction if happens in treated cases of Hansen's disease, can be a determining factor for developing persisters and relapse. Read More

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December 2018

Mycobacterial Infection is Promoted by Neutral Sphingomyelinase 2 Regulating a Signaling Cascade Leading to Activation of β1-Integrin.

Cell Physiol Biochem 2018 Nov 30;51(4):1815-1829. Epub 2018 Nov 30.

Department of Molecular Biology, University of Duisburg-Essen, Essen,

Background/aims: Mycobacteria-induced diseases, especially tuberculosis, cause more than 1 million deaths each year, which is higher than any other single bacterial pathogen. Neutral sphingomyelinase 2 (Nsm2) has been implied in many physiological processes and diseases, but the role of Nsm2 in pathogen-host interactions and mycobacterial infections has barely been studied.

Methods: We investigated the role of the Nsm2/ceramide system in systemic infection of mice and murine macrophages with Mycobacterium bovis Bacillus Calmette-Guérin (BCG) as a model for mycobacterial infection. Read More

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November 2018

Antibody response against Mycobacterium avium complex in cystic fibrosis patients measured by a novel IgG ELISA test.

J Cyst Fibros 2018 Nov 29. Epub 2018 Nov 29.

Department of Clinical Microbiology, Rigshospitalet, Copenhagen, Denmark; Institute of Immunology and Medical Microbiology, University of Copenhagen, Denmark.

Background: Early signs of Mycobacterium avium complex pulmonary disease can be missed in patients with cystic fibrosis due to subclinical infection or delays in mycobacterial culture. The aim of this study was to determine the diagnostic accuracy of a novel enzyme linked immunosorbent assay for immunoglobulin G against Mycobacterium avium complex, which could help stratify patients according to risk.

Methods: A retrospective cross sectional analysis of serum samples from the Copenhagen Cystic Fibrosis Center was performed. Read More

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November 2018

Comparative proteomics identified immune response proteins involved in response to vaccination with heat-inactivated Mycobacterium bovis and mycobacterial challenge in cattle.

Vet Immunol Immunopathol 2018 Dec 29;206:54-64. Epub 2018 Oct 29.

SaBio, Instituto de Investigación en Recursos Cinegéticos IREC CSIC-UCLM-JCCM, Ronda de Toledo s/n, 13005 Ciudad Real, Spain; Department of Veterinary Pathobiology, Center for Veterinary Health Sciences, Oklahoma State University, Stillwater, OK 74078, USA.

There is an imperative need for effective control of bovine tuberculosis (BTB) on a global scale and vaccination of cattle may prove to be pivotal in achieving this. The oral and parenteral use of a heat-inactivated Mycobacterium bovis (M. bovis) vaccine has previously been found to confer partial protection against BTB in several species. Read More

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December 2018

Mycobacterium genavense Infection in a Domestic Ferret (Mustela putorius furo).

Top Companion Anim Med 2018 Dec 10;33(4):119-121. Epub 2018 Oct 10.

Istituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia Romagna (IZSLER), 25124 Brescia, Italy.

Mycobacterium genavense infection was diagnosed in an adult ferret with ptosis of the left eye, a proliferative lesion of the conjunctiva of the nictitating membrane, conjunctival swelling, and tumefaction of the periorbital tissues with a watery ocular discharge and the presence of a retrobulbar mass. The diagnosis was based on characteristic cytology of the retrobulbar mass and left mandibular lymph node that revealed granulomatous inflammation. Ziehl-Neelsen staining showed the presence of positive acid-fast bacilli in the cytoplasm of the macrophages. Read More

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December 2018

Immunological responses of European badgers (Meles Meles) to infection with Mycobacterium bovis.

Comp Immunol Microbiol Infect Dis 2018 Dec 24;61:9-15. Epub 2018 Sep 24.

Animal and Plant Health Agency, Woodham Lane, New Haw, KT15 3NB, United Kingdom. Electronic address:

Mycobacterium bovis is the main cause of bovine tuberculosis and its eradication is proving difficult in many countries because of wildlife reservoirs, including European badgers (Meles meles) in the UK Ireland. Following the development of badger specific immunological reagents, many studies have shown that some aspects of the cellular and serological immune responses of badgers to virulent M. bovis and the attenuated M. Read More

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December 2018
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Serological and molecular evaluation of Mycobacterium avium subspecies paratuberculosis (Johne's disease) infecting riverine-type water buffaloes (Bubalus bubalis) in the Philippines.

Comp Immunol Microbiol Infect Dis 2018 Dec 20;61:24-29. Epub 2018 Nov 20.

Philippine Carabao Center National Headquarters and Gene Pool, Science City of Muñoz 3120, Nueva Ecija, Philippines; Department of Animal Science, College of Agriculture, Central Luzon State University, Science City of Muñoz 3120, Nueva Ecija, Philippines. Electronic address:

Mycobacterium avium subsp. paratuberculosis (MAP) is the causative agent of Johne's disease and a possible cause of Crohn's disease in humans. A total of 70 blood and fecal samples were collected from water buffaloes in selected municipalities of Nueva Ecija for ELISA and qPCR assay. Read More

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December 2018

Structural Basis for Inhibition of Enoyl-[Acyl Carrier Protein] Reductase (InhA) from Mycobacterium tuberculosis.

Curr Med Chem 2018 Dec 3. Epub 2018 Dec 3.

Laboratory of Computational Systems Biology, School of Sciences - Pontifical Catholic University of Rio Grande do Sul (PUCRS), Av. Ipiranga, 6681, Porto Alegre-RS 90619-900. Brazil.

Background: The enzyme trans-enoyl-[acyl carrier protein] reductase (InhA) is a central protein for the development of antitubercular drugs. This enzyme is the target for the pro-drug isoniazid, which is catalyzed by the enzyme catalase-peroxidase (KatG) to become active.

Objective: Our goal here is to review the studies on InhA, starting with general aspects and focusing on the recent structural studies, with emphasis on the crystallographic structures of complexes involving InhA and inhibitors. Read More

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December 2018
3 Reads

Identification of compounds with pH-dependent bactericidal activity against Mycobacterium tuberculosis.

ACS Infect Dis 2018 Dec 1. Epub 2018 Dec 1.

To find new inhibitors of Mycobacterium tuberculosis that have novel mechanisms of action, we miniaturized a high throughput screen to identify compounds which disrupt pH homeostasis. We adapted and validated a 384-well format assay to determine intrabacterial pH using a ratiometric green fluorescent protein. We screened 89k small molecules under non-replicating conditions and confirmed 556 hits that reduced intrabacterial pH (below pH 6. Read More

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December 2018

Improved Biosafety and Biosecurity Measures and/or Strategies to Tackle Laboratory-Acquired Infections and Related Risks.

Int J Environ Res Public Health 2018 Nov 29;15(12). Epub 2018 Nov 29.

Tecnologico de Monterrey, School of Engineering and Sciences, Campus Monterrey, Ave. Eugenio Garza Sada 2501, CP 64849 Monterrey, N.L., Mexico.

Herein, we reviewed laboratory-acquired infections (LAIs) along with their health-related biological risks to provide an evidence base to tackle biosafety/biosecurity and biocontainment issues. Over the past years, a broad spectrum of pathogenic agents, such as bacteria, fungi, viruses, parasites, or genetically modified organisms, have been described and gained a substantial concern due to their profound biological as well as ecological risks. Furthermore, the emergence and/or re-emergence of life-threatening diseases are of supreme concern and come under the biosafety and biosecurity agenda to circumvent LAIs. Read More

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November 2018

Cluster of invasive Mycobacteria chimaera infections following cardiac surgery demonstrating novel clinical features and risks of aortic valve replacement.

Intern Med J 2018 Dec;48(12):1514-1520

Department of Infectious Diseases, Prince of Wales Hospital, Randwick, New South Wales, Australia.

There is a global outbreak of infections due to Mycobacterium chimaera associated with cardiac surgery. The most serious infections involve prosthetic material implantation, and all have followed surgical procedures involving cardiopulmonary bypass. We describe a cluster of four cases following cardiac surgery at a tertiary referral centre in Sydney, Australia. Read More

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December 2018
2 Reads

Diabetes insipidus secondary to tuberculous meningoencephalitis with hypothalamic involvement extending to the hypophysis: a case report.

Rev Soc Bras Med Trop 2018 Nov-Dec;51(6):865-867

Fundação de Medicina Tropical Dr. Heitor Vieira Dourado, Manaus, AM, Brasil.

The involvement of Mycobacterium tuberculosis in the central nervous system (CNS) is an uncommon and devastating manifestation of tuberculosis. We report a case of disseminated tuberculosis presenting as meningoencephalitis, hypothalamic involvement with extension to the hypophysis, and secondary insipidus diabetes diagnosed at autopsy. Read More

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December 2017

Preanalytical conditions can interfere with M. tuberculosis detection by PCR in respiratory samples.

Clinics (Sao Paulo) 2018 Nov 29;73:e410. Epub 2018 Nov 29.

Divisao Pulmonar, Instituto do Coracao (InCor), Hospital das Clinicas HCFMUSP, Faculdade de Medicina, Universidade de Sao Paulo, Sao Paulo, SP, BR.

Objectives: Tuberculosis is one of the most prevalent infections in humans. Although culture is the reference for diagnosis, its sensitivity is compromised, especially in paucibacillary samples. Because polymerase chain reaction (PCR) amplifies mycobacterial DNA, it is more sensitive than culture for the diagnosis of Mycobacterium tuberculosis (Mtb). Read More

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November 2018
1 Read

Screening of healthcare workers for latent tuberculosis infection in the low tuberculosis burden country: QuantiFERON-TB gold in tube test or tuberculin skin test?

Arch Environ Occup Health 2018 Dec 5:1-6. Epub 2018 Dec 5.

b Pediatric Infectious Disease Research Center , Tehran University of Medical Sciences , Tehran , Iran.

Healthcare workers (HCWs) are an important group at increased risk for exposure to various infectious agents including Mycobacterium tuberculosis. Unfortunately, there is very limited data on the prevalence of latent tuberculosis infection (LTBI) among HCWs in Iran. The aim of this study was to analyze the prevalence of LTBI among HCW's of Children Medical Center (CMC), an Iranian referral hospital, and to compare the feasibility of using tuberculin skin test (TST) and QuantiFERON-TB gold in tube test (QFT-GIT) to screen the LTBI among this population. Read More

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December 2018
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Differential HLA Allele Frequency in M. africanum vs. M. tuberculosis in Mali.

HLA 2018 Dec 4. Epub 2018 Dec 4.

University of Sciences, Techniques and Technologies of Bamako (USTTB), Bamako, Mali.

Tuberculosis is caused by Mycobacterium tuberculosis complex (MTBC), however, the distribution and frequency of MTBC lineages and sublineages vary in different parts of the globe. Mycobacterium africanum, a member of MTBC is responsible for a large percentage of TB cases in West Africa, however, it is rarely identified outside of this part of the World. Whether or not differential HLA polymorphism (an important host factor) is contributing to the geographic restriction of M. Read More

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December 2018

Decavanadate Inhibits Mycobacterial Growth More Potently Than Other Oxovanadates.

Front Chem 2018 20;6:519. Epub 2018 Nov 20.

Department of Chemistry, Colorado State University, Fort Collins, CO, United States.

V NMR spectroscopy is used to document, using speciation analysis, that one oxometalate is a more potent growth inhibitor of two Mycobacterial strains than other oxovanadates, thus demonstrating selectivity in its interaction with cells. Historically, oxometalates have had many applications in biological and medical studies, including study of the phase-problem in X-ray crystallography of the ribosome. The effect of different vanadate salts on the growth of () and () was investigated, and speciation was found to be critical for the observed growth inhibition. Read More

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November 2018

Selective Targeting of 4SO--Acetyl-Galactosamine Functionalized Protein Loaded Chitosan Nanoparticle to Macrophages: Correlation With Activation of Immune System.

Front Microbiol 2018 20;9:2469. Epub 2018 Nov 20.

Interdisciplinary Biotechnology Unit, Aligarh Muslim University, Aligarh, India.

In the present study, we investigated potential of chitosan-based nanoparticles (CNPs) to deliver loaded therapeutic molecules to pathogen harboring macrophages. We fabricated stable CNPs employing ionic cross-linking method and evaluated their potential to target RAW 264.7 cells. Read More

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November 2018

The LPG1x family from Leishmania major is constituted of rare eukaryotic galactofuranosyltransferases with unprecedented catalytic properties.

Sci Rep 2018 Dec 4;8(1):17566. Epub 2018 Dec 4.

Institut de Chimie Organique et Analytique, UMR CNRS 7311, Université d'Orléans, Rue de Chartres, BP6759, Orléans, Cedex 02, France.

Galactofuranosyltransferases are poorly described enzymes despite their crucial role in the virulence and the pathogenicity of numerous microorganisms. These enzymes are considered as potential targets for therapeutic action. In addition to the only well-characterised prokaryotic GlfT2 from Mycobacterium tuberculosis, four putative genes in Leishmania major were previously described as potential galactofuranosyltransferases. Read More

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December 2018

Cell death at the cross roads of host-pathogen interaction in Mycobacterium tuberculosis infection.

Tuberculosis (Edinb) 2018 Dec 29;113:99-121. Epub 2018 Sep 29.

Molecular Pathogenesis Laboratory, Department of Biochemistry, School of Life Sciences, University of Hyderabad, Hyderabad, Telangana, India-500046. Electronic address:

Tuberculosis (TB) continues to be the leading cause of death by any single infectious agent, accounting for around 1.7 million annual deaths globally, despite several interventions and support programs by national and international agencies. With the development of drug resistance in Mycobacterium tuberculosis (M. Read More

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December 2018

Drug susceptibility testing of mature Mycobacterium tuberculosis H37Ra and Mycobacterium smegmatis biofilms with calorimetry and laser spectroscopy.

Tuberculosis (Edinb) 2018 Dec 3;113:91-98. Epub 2018 Oct 3.

Center of Biomechanics & Calorimetry Basel (COB), University Basel, C/o Department of Biomedical Engineering (DBE), Gewerbestr. 14, CH-4123, Allschwil, Switzerland. Electronic address:

Biofilms are more resistant to antibiotics and antimicrobial stressors than planktonic bacteria; however, only a limited number of standardized assays enable investigation of this phenomenon. Here, we utilized non-invasive and independent techniques, including isothermal microcalorimetry (IMC) and tunable diode laser absorption spectroscopy (TDLAS), to measure the effect of isoniazid on metabolic activity and respiratory capability of mature Mycobacterium tuberculosis H37Ra (an avirulent strain) and Mycobacterium smegmatis biofilms. We detected only minor changes in metabolic heat production and respiratory rates (O and CO) for mature M. Read More

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December 2018

Polysaccharide rich extract (PRE) from Tinospora cordifolia inhibits the intracellular survival of drug resistant strains of Mycobacterium tuberculosis in macrophages by nitric oxide induction.

Tuberculosis (Edinb) 2018 Dec 26;113:81-90. Epub 2018 Sep 26.

Radiation Medicine Centre, Bhabha Atomic Research Centre, c/o TMH Annexe, Parel, Mumbai, India; Homi Bhabha National Institute, Training School Complex, Anushakti Nagar, Mumbai, India. Electronic address:

Plethora of clinical and scientific information obtained in recent past has strengthened the idea that targeting critical constituents of host immune system may have beneficial outcomes for the treatment of tuberculosis. Macrophages being the primary host for Mycobacterium tuberculosis, offer an attractive target for modulation. Owing to their negligible toxicity, plant derived polysaccharides with the ability to activate macrophages; are suitable candidates for immunomodulation. Read More

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December 2018

In vivo electroporation of a codon-optimized BER DNA vaccine protects mice from pathogenic Mycobacterium tuberculosis aerosol challenge.

Tuberculosis (Edinb) 2018 Dec 10;113:65-75. Epub 2018 Jul 10.

AIDS Institute and Department of Microbiology, State Key Laboratory of Emerging Infectious Diseases, The University of Hong Kong, Li Ka Shing Faculty of Medicine, Hong Kong SAR, PR China; HKU AIDS Institute Shenzhen Research Laboratory and Guangdong Key Laboratory for Emerging Infectious Disease, Shenzhen Third People's Hospital, Guangdong Medical College, Shenzhen, PR China. Electronic address:

DNA vaccines have been extensively studied as preventative and therapeutic interventions for various infectious diseases such as tuberculosis, HIV/AIDS and influenza. Despite promising progresses made, improving the immunogenicity of DNA vaccine remains a technical challenge for clinical development. In this study, we investigated a tuberculosis DNA vaccine BER, which contained a codon-optimized fusion immunogen Ag85B-ESAT-6-Rv2660c for enhanced mammalian cell expression and immunogenicity. Read More

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December 2018
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Chemical classes targeting energy supplying GyrB domain of Mycobacterium tuberculosis.

Tuberculosis (Edinb) 2018 Dec 8;113:43-54. Epub 2018 Sep 8.

Molecular Modeling Lab (MML), Department of Pharmaceutical Sciences and Drug Research, Punjabi University, Patiala, Punjab, 147002, India. Electronic address:

Tuberculosis (TB) is contagious in nature and immunocompromised patients have a higher probability of developing TB. The occurrence of drug resistance, has led to serious health concerns in the management of TB. In order to combat resistant tuberculosis there is an urgent need of identifying new drug targets and new drug combinations for the effective management and reduction in the duration of TB treatment. Read More

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December 2018