2,535 results match your criteria Mutagenesis[Journal]


Inhibitory effect of ochratoxin A on DNMT-mediated flocculation of yeast.

Mutagenesis 2019 Mar 10. Epub 2019 Mar 10.

Division of Genetics and Mutagenesis, National Institute of Health Sciences, 3-25-26 Tonomachi, Kawasaki-ku, Kawasaki-shi, Kanagawa, Japan.

The mycotoxin ochratoxin A (OTA) is considered to be a human carcinogen. However, the mode of its carcinogenetic action has not been elucidated. Recently, it has become evident that epigenetic changes influence the risk of developing cancer. Read More

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https://academic.oup.com/mutage/advance-article/doi/10.1093/
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http://dx.doi.org/10.1093/mutage/gez002DOI Listing
March 2019
3 Reads

Telomere length and genotoxicity in the lung of rats following intragastric exposure to food-grade titanium dioxide and vegetable carbon particles.

Mutagenesis 2019 Mar 10. Epub 2019 Mar 10.

Department of Public Health, Section of Environmental Health, Frederiksberg C, Denmark.

Vegetable carbon (E153) and titanium dioxide (E171) are widely used as black and white food colour additives. The aim of this study was to assess gastrointestinal tight junction and systemic genotoxic effects in rats following exposure to E153 and E171 for 10 weeks by oral gavage once a week. The expression of tight junction proteins was assessed in intestinal tissues. Read More

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http://dx.doi.org/10.1093/mutage/gez003DOI Listing
March 2019
2 Reads

The processes associated with lipid peroxidation in human embryonic lung fibroblasts, treated with polycyclic aromatic hydrocarbons and organic extract from particulate matter.

Mutagenesis 2019 Mar 10. Epub 2019 Mar 10.

Department of Genetic Toxicology and Nanotoxicology, Institute of Experimental Medicine of the Czech Academy of Sciences, Prague, Czech Republic.

Polycyclic aromatic hydrocarbons (PAHs) may cause lipid peroxidation via reactive oxygen species generation. 15-F2t-isoprostane (IsoP), an oxidative stress marker, is formed from arachidonic acid (AA) by a free-radical induced oxidation. AA may also be converted to prostaglandins (PG) by prostaglandin-endoperoxide synthase (PTGS) induced by NF-κB. Read More

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http://dx.doi.org/10.1093/mutage/gez004DOI Listing
March 2019
3 Reads

Genetic variants in microRNAs are associated with cervical cancer risk.

Mutagenesis 2019 Mar 10. Epub 2019 Mar 10.

Clinical Laboratory, Affiliated Tumor Hospital of Nantong University (Nantong Tumor Hospital), Nantong, China.

Because genetic variants in microRNAs (miRNAs) or their surrounding regions can alter miRNA processing, expression and final biological function, we investigated whether miRNA single-nucleotide polymorphisms (SNPs) are associated with cervical cancer (CC) susceptibility. Common miRNA SNPs (i.e. Read More

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http://dx.doi.org/10.1093/mutage/gez005DOI Listing
March 2019
2 Reads

Comparative study of toxicological assessment of yttrium oxide nano- and microparticles in Wistar rats after 28 days of repeated oral administration.

Mutagenesis 2019 Feb 9. Epub 2019 Feb 9.

Toxicology Unit, Applied Biology Division, CSIR - Indian Institute of Chemical Technology, Hyderabad, Telangana, India.

Despite their enormous advantages, nanoparticles (NPs) have elicited disquiet over their safety. Among the numerous NPs, yttrium oxide (Y2O3) NPs are utilised in many applications. However, knowledge about their toxicity is limited, and it is imperative to investigate their potential adverse effects. Read More

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http://dx.doi.org/10.1093/mutage/gey044DOI Listing
February 2019
4 Reads

Vitamin D3 as adjuvant in the treatment of type 2 diabetes mellitus: modulation of genomic and biochemical instability.

Mutagenesis 2019 Feb 6. Epub 2019 Feb 6.

Translational Biomedicine Laboratory, Graduate Programme of Health Sciences, University of Southern Santa Catarina, UNESC, Criciúma, Santa Catarina, Brazil.

Type 2 diabetes mellitus has undergone a worldwide growth in incidence in the world and has now acquired epidemic status. There is a strong link between type 2 diabetes and vitamin D deficiency. Because vitamin D has beneficial effects on glucose homeostasis, the aim of this study was to evaluate the influence of vitamin D3 supplementation on the modulation of glycaemic control and other metabolic effects, as well as modulation of genomic instability in patients with type 2 diabetes. Read More

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http://dx.doi.org/10.1093/mutage/gez001DOI Listing
February 2019
2 Reads

In silico model for mutagenicity (Ames test), taking into account metabolism.

Mutagenesis 2019 03;34(1):41-48

Department of Environmental Health Sciences, Istituto di Ricerche Farmacologiche Mario Negri IRCCS, Via Giuseppe La Masa, Milan, Italy.

Bacterial reverse mutation test is one of the most common methods used to address genotoxicity. The experimental test is designed to include a step to simulate mammalian metabolism. The most common metabolic activation system is the incubation with S9 fraction prepared from the livers of rodents. Read More

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http://dx.doi.org/10.1093/mutage/gey045DOI Listing
March 2019
1 Read

A practice of expert review by read-across using QSAR Toolbox.

Mutagenesis 2019 03;34(1):49-54

Division of Pharmacopoeia and Standards for Drugs, Pharmaceuticals and Medical Devices Agency, Shin-Kasumigaseki Building, Kasumigaseki, Chiyoda-ku, Tokyo, Japan.

The International Council for Harmonisation of Technical Requirement for Pharmaceuticals for Human Use (ICH) M7 guideline on 'Assessment and Control of DNA Reactive (Mutagenic) Impurities in Pharmaceuticals to Limit Potential Carcinogenic Risk' provides the application of two types of quantitative structure-activity relationship (QSAR) systems (rule- and statistics-based) as an alternative to the Ames test for evaluating the mutagenicity of impurities in pharmaceuticals. M7 guideline also states that the expert reviews can be applied when the outcomes of the two QSAR analyses show any conflicting or inconclusive prediction. However, the guideline does not provide any information of how to conduct expert reviews. Read More

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http://dx.doi.org/10.1093/mutage/gey046DOI Listing
March 2019
3 Reads

Quantitative analysis of γH2AX reveals distinct responses in multiple mouse organs after administration of mitomycin C or ethyl methanesulfonate.

Mutagenesis 2018 12;33(5-6):371-378

Safety Evaluation Center, Ecology and Quality Management Division, CSR Division, FUJIFILM Corporation, 210 Nakanuma, Minamiashigara, Kanagawa, Japan.

Ser139-phosphorylated H2AX (γH2AX) is a functional biomarker of DNA double-strand breaks. However, its conventional detection for in vivo samples relies on immunological methods using anti-γH2AX antibodies, making quantitative analysis difficult. Here, we established an absolute γH2AX quantification in vivo method for multiple organs in mice using liquid chromatography-triple quadrupole tandem mass spectrometry. Read More

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http://dx.doi.org/10.1093/mutage/gey040DOI Listing
December 2018
2 Reads

An Adaption of Human-Induced Hepatocytes to In Vitro Genetic Toxicity Tests.

Mutagenesis 2018 Dec 24. Epub 2018 Dec 24.

Hongqiao International Institute of Medicine, Shanghai Tong Ren Hospital and Faculty of Public Health, Shanghai Jiao Tong University School of Medicine, Shanghai, China.

Metabolic activation is essential in standard in vitro genotoxicity test systems. At present, there is a lack of suitable cell models that can express the major characteristics of liver function for predicting substance toxicity in humans. Human-induced hepatocytes (hiHeps), which have been generated from fibroblasts by lentiviral expression of liver transcription factors, can express hepatic gene programs and can be expanded in vitro and display functional characteristics of mature hepatocytes, including cytochrome P450 enzyme activity and biliary drug clearance. Read More

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https://academic.oup.com/mutage/advance-article/doi/10.1093/
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http://dx.doi.org/10.1093/mutage/gey041DOI Listing
December 2018
18 Reads
2.793 Impact Factor

Validity of reported indicators of pesticide exposure and relevance for cytotoxic and genotoxic effects on buccal cells.

Mutagenesis 2018 Dec 26. Epub 2018 Dec 26.

Department of Environmental Health, Center for Public Health, Medical University of Vienna, Kinderspitalgasse, Vienna, Austria.

Higher rates of nuclear anomalies in buccal cells of coffee workers exposed to pesticides in comparison to their unexposed peers were detected in our field study. Here, we extend our findings by examining other exposure indicators in this sample. Occupational exposure of 38 exposed and 33 non-exposed farmworkers was assessed as exposure days in the last month (0-25 days) and as years of exposure (0-47 years). Read More

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http://dx.doi.org/10.1093/mutage/gey043DOI Listing
December 2018
2 Reads

A decision tree-based integrated testing strategy for tailor-made carcinogenicity evaluation of test substances using genotoxicity test results and chemical spaces.

Mutagenesis 2019 03;34(1):101-109

R&D, Safety Science Research, Kao Corporation, Ichikai-Machi, Haga-Gun, Tochigi, Japan.

Genotoxicity evaluation has been widely used to estimate the carcinogenicity of test substances during safety evaluation. However, the latest strategies using genotoxicity tests give more weight to sensitivity; therefore, their accuracy has been very low. For precise carcinogenicity evaluation, we attempted to establish an integrated testing strategy for the tailor-made carcinogenicity evaluation of test materials, considering the relationships among genotoxicity test results (Ames, in vitro mammalian genotoxicity and in vivo micronucleus), carcinogenicity test results and chemical properties (molecular weight, logKow and 179 organic functional groups). Read More

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http://dx.doi.org/10.1093/mutage/gey039DOI Listing
March 2019
3 Reads

Predicting Ames Mutagenicity Using Conformal Prediction in the Ames/QSAR International Challenge Project.

Mutagenesis 2019 03;34(1):33-40

Computer Learning Research Centre, Royal Holloway, University of London Egham, Surrey, UK.

Valid and predictive models for classifying Ames mutagenicity have been developed using conformal prediction. The models are Random Forest models using signature molecular descriptors. The investigation indicates, on excluding not-strongly mutagenic compounds (class B), that the validity for mutagenic compounds is increased for the predictions based on both public and the Division of Genetics and Mutagenesis, National Institute of Health Sciences of Japan (DGM/NIHS) data while less so when using only the latter data source. Read More

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http://dx.doi.org/10.1093/mutage/gey038DOI Listing
March 2019
2 Reads

Comparative proteomic analysis of human peripheral blood mononuclear cells indicates adaptive response to low-dose radiation in individuals from high background radiation areas of Kerala.

Mutagenesis 2018 12;33(5-6):359-370

Radiation Signaling Group, Radiation Biology & Health Sciences Division, Bhabha Atomic Research Centre, Mumbai, India.

There remain significant uncertainties in estimation of risks with low doses of radiation. The small coastal belt in the southwestern state of Kerala, India, extending from Neendakara in the south to Purakkad in the north is one of the most extensively studied high-level natural background radiation areas (HLNRAs) of the world to address these concerns. The natural radioactivity here is due to occurrence of monazite sand bearing placer deposits along the coastline. Read More

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http://dx.doi.org/10.1093/mutage/gey036DOI Listing
December 2018
3 Reads

Apoptosis and genome instability in children with autoimmune diseases.

Mutagenesis 2018 12;33(5-6):351-357

Department of Pediatrics, Faculty of Medical Sciences, University of Kragujevac, Kragujevac, Serbia.

As apoptosis and genome instability in children with autoimmune diseases (AIDs) are insufficiently investigated, we aimed to analyse them in peripheral blood lymphocytes (PBLs) of children and adolescents with Hashimoto's thyroiditis (HT), Graves' disease (GD) and type 1 diabetes mellitus (T1DM), including possible factors that could affect their occurrence. The study population included 24 patients and 19 healthy controls. Apoptotic cells were detected using an Annexin V-FITC/7-AAD kit. Read More

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http://fdslive.oup.com/www.oup.com/pdf/production_in_progres
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http://dx.doi.org/10.1093/mutage/gey037DOI Listing
December 2018
29 Reads

Validation of the performance of TIMES genotoxicity models with EFSA pesticide data.

Mutagenesis 2019 03;34(1):83-90

Laboratory of Mathematical Chemistry (LMC), University "Prof. D-R Assen Zlatarov"-Burgas, Burgas, Bulgaria.

This study validates the performance of the TIssue MEtabolism Simulator (TIMES) genotoxicity models with data on pesticide chemicals included in a recently released European Food Safety Authority (EFSA) genotoxicity database. The EFSA database is biased towards negative chemicals. A comparison of substances included in the EFSA database and TIMES genotoxicity databases showed that the majority of the EFSA pesticides is not included in the TIMES genotoxicity databases and, thus, out of the applicability domains of the current TIMES models. Read More

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http://dx.doi.org/10.1093/mutage/gey035DOI Listing
March 2019
6 Reads

Improvement of quantitative structure-activity relationship (QSAR) tools for predicting Ames mutagenicity: outcomes of the Ames/QSAR International Challenge Project.

Mutagenesis 2019 03;34(1):3-16

Molecular Networks GmbH and Altamira LLC, Neumeyerstrasse Nürnberg, Germany and Candlewood Drive, Columbus, OH, USA.

The International Conference on Harmonization (ICH) M7 guideline allows the use of in silico approaches for predicting Ames mutagenicity for the initial assessment of impurities in pharmaceuticals. This is the first international guideline that addresses the use of quantitative structure-activity relationship (QSAR) models in lieu of actual toxicological studies for human health assessment. Therefore, QSAR models for Ames mutagenicity now require higher predictive power for identifying mutagenic chemicals. Read More

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http://dx.doi.org/10.1093/mutage/gey031DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6402315PMC
March 2019
5 Reads

Important considerations for the validation of QSAR models for in vitro mutagenicity.

Mutagenesis 2019 03;34(1):25-32

Lhasa Limited, Granary Wharf House, Leeds, UK.

While high-level performance metrics generated from the validation of quantitative structure-activity relationship (QSAR) systems can provide valuable information on how well these models perform and where they need to be improved, they require appropriate interpretation. There is no universal performance metric which will answer all of the questions a user might ask relating to a model, and therefore, a combination of metrics should usually be considered. Furthermore, results may vary according to the chemical space being used to validate a model, and, in some cases, it may be the validation data which is lacking or ambiguous rather than the prediction being made. Read More

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https://academic.oup.com/mutage/advance-article/doi/10.1093/
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http://dx.doi.org/10.1093/mutage/gey034DOI Listing
March 2019
13 Reads

Computing similarity between structural environments of mutagenicity alerts.

Mutagenesis 2019 03;34(1):55-65

MultiCASE Inc., Chagrin Blvd, Suite, Beachwood, OH, USA.

This article describes a method to generate molecular fingerprints from structural environments of mutagenicity alerts and calculate similarity between them. This approach was used to improve classification accuracy of alerts and for searching structurally similar analogues of an alerting chemical. It builds fingerprints using molecular fragments from the vicinity of the alerts and automatically accounts for the activating and deactivating/mitigating features of alerts needed for accurate predictions. Read More

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http://dx.doi.org/10.1093/mutage/gey032DOI Listing
March 2019
3 Reads

Extrapolation of in vitro structural alerts for mutagenicity to the in vivo endpoint.

Mutagenesis 2019 03;34(1):111-121

Lhasa Limited, Granary Wharf House, Leeds, UK.

As part of the hazard and risk assessment of chemicals in man, it is important to assess the ability of a chemical to induce mutations in vivo. Because of the commonalities in the molecular initiating event, mutagenicity in vitro can correlate well to the in vivo endpoint for certain compound classes; however, the difficulty lies in identifying when this correlation holds true. In silico alerts for in vitro mutagenicity may therefore be used as the basis for alerts for mutagenicity in vivo where an expert assessment is carried out to establish the relevance of the correlation. Read More

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http://dx.doi.org/10.1093/mutage/gey030DOI Listing
March 2019
3 Reads

Data-based review of QSARs for predicting genotoxicity: the state of the art.

Mutagenesis 2019 03;34(1):17-23

Department of Health and Environment, Istituto Superiore di Sanità, Viale Regina Elena, Rome, Italy.

With the aim of providing faster, more economical, animal-free tools to predict toxicity, quantitative structure-activity relationships (QSAR) approaches are increasingly applied to the chemical risk assessment-in particular genotoxicity and carcinogenicity. The more recent period of time has witnessed refinements of the predictive systems, with the collection of larger training sets and continued fine-tuning, together with an increased interest for the use of QSAR by regulatory authorities. This literature review provides an updated snapshot of the present state of the art in the evaluation of QSAR methods as applied to genotoxicity. Read More

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https://academic.oup.com/mutage/advance-article/doi/10.1093/
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http://dx.doi.org/10.1093/mutage/gey028DOI Listing
March 2019
20 Reads

A novel FAM83H mutation in one Chinese family with autosomal-dominant hypocalcification amelogenesis imperfecta.

Mutagenesis 2018 10;33(4):333-340

Department of Preventive Dentistry, Peking University School and Hospital of Stomatology, National Engineering Laboratory for Digital and Material Technology of Stomatology, Beijing Key Laboratory of Digital Stomatology, Beijing, PR China.

Autosomal-dominant hypocalcification amelogenesis imperfecta (ADHCAI) is characterized by soft enamel that easily disintegrates and exposed dark dentin. ADHCAI is caused by mutations in a gene called family with sequence similarity 83 member H (FAM83H). To investigate the molecular genetics of ADHCAI, a Chinese family in which three generations exhibited ADHCAI was recruited. Read More

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http://dx.doi.org/10.1093/mutage/gey019DOI Listing
October 2018
6 Reads

Induction of in vivo Pig-a gene mutation but not micronuclei by 5-(2-chloroethyl)-2'-deoxyuridine, an antiviral pyrimidine nucleoside analogue.

Mutagenesis 2018 12;33(5-6):343-350

Preclinical Safety, Novartis Institutes for Biomedical Research, Novartis Pharma AG, Basel, Switzerland.

5-(2-Chloroethyl)-2'-deoxyuridine (CEDU) was developed as an antiviral drug. It has been studied in a number of in vitro and in vivo genotoxicity assays and is considered an unusual nucleoside analogue owing to its potent mutagenic potential, with little to no measurable clastogenic activity. Given this atypical profile, CEDU represented an interesting compound for evaluating the in vivo Pig-a gene mutation assay, a test that is undergoing extensive validation for regulatory safety applications. Read More

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http://dx.doi.org/10.1093/mutage/gey029DOI Listing
December 2018
3 Reads

Chemotherapy-induced genotoxic damage to bone marrow cells: long-term implications.

Mutagenesis 2018 09;33(3):241-251

Centre for Research in Biosciences, University of the West of England, Coldharbour Lane, Bristol, UK.

Mesenchymal stem/stromal cells (MSCs) within the bone marrow (BM) are vitally important in forming the micro-environment supporting haematopoiesis after myeloablative chemotherapy. MSCs are known to be damaged phenotypically and functionally by chemotherapy; however, to the best our knowledge, the persistence of genotoxic effects of chemotherapy on the BM micro-environment has not been studied. We therefore aimed to evaluate genotoxic effects of chemotherapy on the BM both in vitro and in vivo, using the comet and micronucleus assays, focussing on the persistence of DNA lesions that may contribute to complications in the patient. Read More

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http://dx.doi.org/10.1093/mutage/gey014DOI Listing
September 2018
31 Reads

Radiation dosimetry and repair kinetics of DNA damage foci in mouse pachytene spermatocyte and round spermatid stages.

Mutagenesis 2018 09;33(3):231-239

Cytogenetics Laboratory, Department of Zoology, Centre of Advanced Study, India.

Accurate quantification of DNA double strand breaks (DSB) in testicular germ cells is difficult because of cellular heterogeneity and the presence of endogenous γH2AX. Here, we used confocal microscopy to quantify DNA damage and repair kinetics following γ-irradiation (0.5-4 Gy) in three major mouse male germ cell stages, early and late pachytene spermatocytes and round spermatids (RSs), following a defined post irradiation time course. Read More

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https://academic.oup.com/mutage/article/33/3/231/4993083
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http://dx.doi.org/10.1093/mutage/gey007DOI Listing
September 2018
4 Reads

The impact of p53 function on the metabolic activation of the carcinogenic air pollutant 3-nitrobenzanthrone and its metabolites 3-aminobenzanthrone and N-hydroxy-3-aminobenzanthrone in human cells.

Mutagenesis 2018 10;33(4):311-321

Department of Analytical, Environmental and Forensic Sciences, MRC-PHE Centre for Environment and Health, King's College London, London, UK.

The tumour suppressor p53, encoded by TP53, is a key player in a wide network of signalling pathways. We investigated its role in the bioactivation of the environmental carcinogen 3-nitrobenzanthrone (3-NBA)found in diesel exhaust and its metabolites 3-aminobenzanthrone (3-ABA) and N-hydroxy-3-aminobenzanthrone (N-OH-3-ABA) in a panel of isogenic human colorectal HCT116 cells differing only with respect to their TP53 status [i.e. Read More

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http://dx.doi.org/10.1093/mutage/gey025DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6180618PMC
October 2018
4 Reads

Investigating FlowSight® imaging flow cytometry as a platform to assess chemically induced micronuclei using human lymphoblastoid cells in vitro.

Mutagenesis 2018 10;33(4):283-289

Institute of Life Science, Swansea University Medical School, Swansea University, Swansea.

Use of imaging flow cytometry to assess induced DNA damage via the cytokinesis block micronucleus (CBMN) assay has thus far been limited to radiation dosimetry in human lymphocytes using high end, 'ImageStream X' series imaging cytometers. Its potential to enumerate chemically induced DNA damage using in vitro cell lines remains unexplored. In the present manuscript, we investigate the more affordable FlowSight® imaging cytometry platform to assess in vitro micronucleus (MN) induction in the human lymphoblastoid TK6 and metabolically competent MCL-5 cells treated with Methyl Methane Sulfonate (MMS) (0-5 µg/ml), Carbendazim (0-1. Read More

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http://dx.doi.org/10.1093/mutage/gey021DOI Listing
October 2018
12 Reads
2.793 Impact Factor

Rad54/Rad54B deficiency is associated to increased chromosome breakage in mouse spermatocytes.

Mutagenesis 2018 10;33(4):323-332

Laboratory of Biosafety and Risk Assessment, ENEA CR Casaccia, Rome, Italy.

Rad54 protein is a key player of the homologous recombination pathway, required for deposition and stabilisation of Rad51 foci at double strand breaks. Its role at the meiotic prophase, when double strand breaks are physiologically introduced to allow recombination, is well described. However, the hypothesis that Rad54 deficiency affect chromosome integrity of germ cells in unirradiated and irradiated animals has not been tested yet. Read More

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http://dx.doi.org/10.1093/mutage/gey027DOI Listing
October 2018
2 Reads

Extending (Q)SARs to incorporate proprietary knowledge for regulatory purposes: is aromatic N-oxide a structural alert for predicting DNA-reactive mutagenicity?

Mutagenesis 2019 03;34(1):67-82

Leadscope, Inc., Columbus, OH, USA.

(Quantitative) structure-activity relationship or (Q)SAR predictions of DNA-reactive mutagenicity are important to support both the design of new chemicals and the assessment of impurities, degradants, metabolites, extractables and leachables, as well as existing chemicals. Aromatic N-oxides represent a class of compounds that are often considered alerting for mutagenicity yet the scientific rationale of this structural alert is not clear and has been questioned. Because aromatic N-oxide-containing compounds may be encountered as impurities, degradants and metabolites, it is important to accurately predict mutagenicity of this chemical class. Read More

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https://academic.oup.com/mutage/advance-article/doi/10.1093/
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http://dx.doi.org/10.1093/mutage/gey020DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6402318PMC
March 2019
18 Reads

Effects of ammonia on apoptosis and oxidative stress in bovine mammary epithelial cells.

Mutagenesis 2018 10;33(4):291-299

College of Animal Sciences, Jilin University, Changchun, Jilin, P.R. China.

Ammonia, produced mainly from the deamination of amino acids and glutamine, is one of the major toxic components in blood and tissues that may affect bovine health. However, the physiological and pathological roles of ammonia in the mammary glands are not understood clearly. In the present study, the bovine mammary epithelial cell line (MAC-T) was utilised as an in vitro model to determine the effects of ammonia on bovine mammary gland. Read More

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http://dx.doi.org/10.1093/mutage/gey023DOI Listing
October 2018
3 Reads

Mutations induced by 8-oxo-7,8-dihydroguanine in WRN- and DNA polymerase λ-double knockdown cells.

Mutagenesis 2018 10;33(4):301-310

College of Pharmaceutical Sciences, Matsuyama University, Matsuyama, Japan.

Reactive oxygen species generate 8-oxo-7,8-dihydroguanine (GO, 8-hydroxyguanine), which induces G:C→T:A transversion mutations. The knockdowns of the protein responsible for Werner syndrome (WRN), a cancer-associated DNA helicase, and DNA polymerase (pol) λ, a WRN-interacting DNA pol, cause untargeted base-substitution mutations (action-at-a-distance mutations). To examine the consequences of the dual reductions of WRN and pol λ for the mutations caused by GO, siRNAs against both proteins were introduced into human U2OS cells. Read More

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http://dx.doi.org/10.1093/mutage/gey024DOI Listing
October 2018
2 Reads

Telomere shortening and aortic plaque progression in Apoliprotein E knockout mice after pulmonary exposure to candle light combustion particles.

Mutagenesis 2018 09;33(3):253-261

Department of Public Health, Section of Environmental Health, University of Copenhagen, Denmark.

Particles from burning candles contribute to the overall indoor exposure to particulate matter (PM). However, little is known about the effects of indoor sources of particles on cardiovascular disease endpoints. This study investigated the effect of pulmonary exposure to particles from combustion of candles and progression of atherosclerosis. Read More

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http://dx.doi.org/10.1093/mutage/gey015DOI Listing
September 2018
2 Reads

Geraniin selectively promotes cytostasis and apoptosis in human colorectal cancer cells by inducing catastrophic chromosomal instability.

Mutagenesis 2018 10;33(4):271-281

School of Life Sciences, The Engineering Research Center of Sustainable Development and Utilization of Biomass Energy, Yunnan Normal University, Kunming, Yunnan, China.

Homeostasis of chromosomal instability (CIN) facilitates the origin and evolution of abnormal karyotypes that are critical for the survival and proliferation of cancer cells, but excessive CIN can result in cellular toxicity. Geraniin is a multifunctional ellagitannin found in some species of Geranium and Phyllanthus. We employed the cytokinesis-block micronucleus cytome assay to evaluate the CIN, nuclear division index (NDI) and apoptosis induced by geraniin in human colorectal adenocarcinoma cells (Colo205 and Colo320) and human colon mucosal epithelial cells (NCM460). Read More

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http://dx.doi.org/10.1093/mutage/gey016DOI Listing
October 2018
10 Reads
2.793 Impact Factor

In silico prediction of chromosome damage: comparison of three (Q)SAR models.

Mutagenesis 2019 03;34(1):91-100

Division of Genetics and Mutagenesis, National Institute of Health Sciences, Tonomachi, Kawasaki, Kanagawa, Japan.

Two major endpoints for genotoxicity tests are gene mutation and chromosome damage (CD), which includes clastogenicity and aneugenicity detected by chromosomal aberration (CA) test or micronucleus (MN) test. Many in silico prediction systems for bacterial mutagenicity (i.e. Read More

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http://dx.doi.org/10.1093/mutage/gey017DOI Listing
March 2019
2 Reads

In silico approaches to genetic toxicology: progress and future.

Authors:
Romualdo Benigni

Mutagenesis 2019 03;34(1):1-2

Alpha-Pretox, Via Giovanni Pascoli 1, 00184, Rome, Italy.

Computational toxicology, also called 'in silico toxicology', is based on scientific knowledge gained from different scientific fields and on the premise that the toxicity of a chemical, depending on its intrinsic nature, can be predicted from its molecular structure and inferred from the properties of similar compounds whose activities are known. With the aim of providing faster, more economical, animal-free tools for predicting toxicity, the 'old' and well established science of Structure-Activity Relationships plays a crucial role, with increasing applications to the assessment of chemical genotoxicity and carcinogenicity. The development of the Structure-Activity Relationships algorithms is a continuous process, and new models, as well as newer versions of applications, are continuously becoming available. Read More

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http://dx.doi.org/10.1093/mutage/gey018DOI Listing
March 2019
2 Reads

A novel IRF6 mutation causing non-syndromic cleft lip with or without cleft palate in a pedigree.

Mutagenesis 2018 09;33(3):195-202

Central Laboratory, Peking University School and Hospital of Stomatology, Beijing, PR China.

Non-syndromic cleft lip with or without cleft palate (NSCLP) is the most common congenital craniofacial malformation, and its harmful influence on affected individuals is apparent. Despite many studies, the causative genes and their mechanisms are not completely clear. We recruited a Han Chinese NSCLP family and explored the causative variant in this pedigree. Read More

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http://dx.doi.org/10.1093/mutage/gey012DOI Listing
September 2018
3 Reads

Hyperthermia enhances methyl methanesulfonate-induced adaptive response in meiotic cells of grasshopper Poecilocerus pictus.

Mutagenesis 2018 09;33(3):215-224

Department of Zoology, Davanagere University, Shivagangothri, Davanagere, Karnataka, India.

To understand the role of hyperthermia (HT) in adaptive response, methyl methanesulfonate (MMS) adapted meiotic cells of Poecilocerus pictus were used. Poecilocerus pictus were treated with conditioning (L) or challenging (H) dose of MMS and 2-h time lag (TL) between these doses (L-2h-H) (combined) was employed. Different treatment schedules were used to analyse the influence of HT on MMS-induced adaptive response namely pre; inter; post-treatment and cross-adaptation. Read More

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http://dx.doi.org/10.1093/mutage/gey009DOI Listing
September 2018
2 Reads

RUNX2 mutation reduces osteogenic differentiation of dental follicle cells in cleidocranial dysplasia.

Mutagenesis 2018 09;33(3):203-214

Department of Preventive Dentistry, Peking University School and Hospital of Stomatology, National Engineering Laboratory for Digital and Material Technology of Stomatology, Beijing Key Laboratory of Digital Stomatology, Haidian District, Beijing, PR China.

Disturbed permanent tooth eruption is common in cleidocranial dysplasia (CCD), a skeletal disorder caused by heterozygous mutation of RUNX2, but the mechanism underlying is still unclear. As it is well known that dental follicle cells (DFCs) play a critical role in tooth eruption, the changed biological characteristics of DFCs might give rise to disturbance of permanent tooth eruption in CCD patients. Thus, primary DFCs from one CCD patient and normal controls were collected to investigate the effect of RUNX2 mutation on the bone remodeling activity of DFCs and explore the mechanism of impaired permanent tooth eruption in this disease. Read More

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http://dx.doi.org/10.1093/mutage/gey010DOI Listing
September 2018
5 Reads

Prevalence and prognostic relevance of BrafV600E mutation in colorectal carcinomas from Kashmir (North India) valley.

Mutagenesis 2018 09;33(3):225-230

Government Medical College and Associated Hospitals, Karanagar, Srinagar, Jammu and Kashmir, India.

Molecular studies have implicated mutant B-type Raf kinase (BRAFMut) of MAP-kinase signalling pathway in the pathogenesis of several cancers including colorectal cancer. Recently, the prognostic and therapeutic relevance of the most frequent BRAFV600E mutation also has been highlighted in colorectal carcinomas (CRC). Thus, the aim of this study was to investigate the prevalence of BRAFV600E mutation and to determine the correlation between this mutation and indicators of poor prognosis and outcome in patients with CRCs from Kashmir, North India. Read More

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http://dx.doi.org/10.1093/mutage/gey008DOI Listing
September 2018
4 Reads
2.793 Impact Factor

A critical appraisal of the sensitivity of in vivo genotoxicity assays in detecting human carcinogens.

Mutagenesis 2018 04;33(2):179-193

Federal Institute for Drugs and Medical Devices (BfArM), Kurt-Georg-Kiesinger-Allee, Bonn, Germany.

Genotoxicity testing is an important part of standard safety testing strategies. Animal studies have always been a key component, either as a mandatory part of the regulatory test battery, or to follow-up questionable in vitro findings. The strengths and weaknesses of in vivo assays is a continuous matter of debate, including their capacity to predict (human) carcinogenicity. Read More

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http://dx.doi.org/10.1093/mutage/gey005DOI Listing
April 2018
9 Reads

Expression and promoter methylation status of two DNA repair genes in leukocytes from patients undergoing propofol or isoflurane anaesthesia.

Mutagenesis 2018 04;33(2):147-152

UNESP - São Paulo State University, Medical School, Professor Mário Rubens G. Montenegro Av., Botucatu - SP, Brazil.

Despite the widespread use of the anaesthetics propofol (PROP) and isoflurane (ISO), data about their toxicogenomic potential and interference in epigenetic events are unknown. This study evaluated the expression and methylation profile of two important DNA-repair genes (XRCC1 and hOGG1) in 40 patients undergoing elective and minimally invasive surgery (tympanoplasty and septoplasty) under ISO or PROP anaesthesia. The endpoints were examined at three sampling times: before anaesthesia (T0), 2 h after the beginning of anaesthesia (T2) and 24 h after the beginning of surgery (T24). Read More

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http://dx.doi.org/10.1093/mutage/gey001DOI Listing
April 2018
7 Reads

Chronic occupational exposure endured by tobacco farmers from Brazil and association with DNA damage.

Mutagenesis 2018 04;33(2):119-128

Laboratory of Toxicological Genetics, Post-Graduate Program in Cellular and Molecular Biology Applied to Health (PPGBioSaúde), Lutheran University of Brazil (ULBRA), Av. Farroupilha, São José, Canoas, Rio Grande do Sul, Brazil.

Tobacco farming is an important economic income in Brazil, although it has been challenged as regard the occupational exposure to both pesticides and nicotine endured by farmers. Chronic occupational exposure to complex mixtures can lead to health hazardous. We examined genomic instability and epigenetic changes in tobacco farmers occupationally exposed to pesticide mixtures and nicotine at tobacco fields. Read More

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http://dx.doi.org/10.1093/mutage/gex045DOI Listing
April 2018
18 Reads

Size-dependent genotoxicity of silver, gold and platinum nanoparticles studied using the mini-gel comet assay and micronucleus scoring with flow cytometry.

Mutagenesis 2018 02;33(1):77-85

Institute of Environmental Medicine, Karolinska Institutet, Nobels väg, Stockholm, Sweden.

Metallic nanoparticles (NPs) are promising nanomaterials used in different technological solutions as well as in consumer products. Silver (Ag), gold (Au) and platinum (Pt) represent three metallic NPs with current or suggested use in different applications. Pt is also used as vehicle exhaust catalyst leading to a possible exposure via inhalation. Read More

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http://dx.doi.org/10.1093/mutage/gex027DOI Listing
February 2018
8 Reads

Role of cerium oxide nanoparticle-induced autophagy as a safeguard to exogenous H2O2-mediated DNA damage in tobacco BY-2 cells.

Mutagenesis 2018 04;33(2):161-177

Plant Molecular Cytogenetics Laboratory, Centre of Advanced Study, Department of Botany, University of Calcutta, Kolkata, West Bengal, India.

The effect of cerium oxide nanoparticle (CeNP) in plants has elicited substantial controversy. While some investigators have reported that CeNP possesses antioxidant properties, others observed CeNP to induce reactive oxygen species (ROS). In spite of considerable research carried out on the effects of CeNP in metazoans, fundamental studies that can unveil its intracellular consequences linking ROS production, autophagy and DNA damage are lacking in plants. Read More

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http://dx.doi.org/10.1093/mutage/gey004DOI Listing
April 2018
7 Reads