2 results match your criteria Mucopolysaccharidoses Types I-VII

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Diagnostic screening for mucopolysaccharidoses types I-VII by fluorophore-labelled carbohydrate PAGE.

J Inherit Metab Dis 1996 ;19(2):263-6

Kaiser Permanente Regional Metabolic Laboratory, North Hollywood 91601, USA.

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October 1996
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Optimization of electroporation for transfection of human fibroblast cell lines with origin-defective SV40 DNA: development of human transformed fibroblast cell lines with mucopolysaccharidoses (I-VII)

Cell Struct Funct 1992 Apr;17(2):123-8

Department of Pediatrics, Gifu University School of Medicine, Japan.

To simplify the process of transfection of human fibroblasts and to acquire a suitable number of transformants, we investigated experimental conditions of electric pulse-induced transfection of human fibroblasts using origin-defective simian virus 40 DNA (SV40 (ori-) DNA). Voltage, pulse duration, number of pulses and the concentration of SV40 (ori-) DNA led to the formation of 10 to 30 foci/25 cm2 6 weeks after transfection, using 2 to 3 x 10(6) cells and a square wave pulse generator. Optimal condition was determined to be 2 or 3 pulses at a voltage of 1500 to 2000 V/0. Read More

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April 1992
2 Reads
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