753 results match your criteria Monkeypox


Identifying and Tracking Low-Frequency Virus-Specific TCR Clonotypes Using High-Throughput Sequencing.

Cell Rep 2018 Nov;25(9):2369-2378.e4

Department of Molecular Microbiology and Immunology, Saint Louis University, Saint Louis, MO 63104, USA. Electronic address:

Tracking antigen-specific T cell responses over time within individuals is difficult because of lack of knowledge of antigen-specific TCR sequences, limitations in sample size, and assay sensitivities. We hypothesized that analyses of high-throughput sequencing of TCR clonotypes could provide functional readouts of individuals' immunological histories. Using high-throughput TCR sequencing, we develop a database of TCRβ sequences from large cohorts of mice before (naive) and after smallpox vaccination. Read More

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November 2018

Assessing the Surveillance System for Priority Zoonotic Diseases in the Democratic Republic of the Congo, 2017.

Health Secur 2018 ;16(S1):S44-S53

Pia D. M. MacDonald, PhD, CPH, is a Senior Epidemiologist, Biostatistics and Epidemiology, RTI International, Durham, North Carolina.

High-functioning communicable disease surveillance systems are critical for public health preparedness. Countries that cannot quickly detect and contain diseases are a risk to the global community. The ability of all countries to comply with the International Health Regulations is paramount for global health security. Read More

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January 2018
2 Reads

Monkeypox - Enhancing public health preparedness for an emerging lethal human zoonotic epidemic threat in the wake of the smallpox post-eradication era.

Int J Infect Dis 2018 Nov 16;78:78-84. Epub 2018 Nov 16.

Division of Infection and Immunity, Center for Clinical Microbiology, University College London, United Kingdom; The National Institute of Health Research Biomedical Research Centre at UCL Hospitals, London, United Kingdom. Electronic address:

The identification of monkeypox in 3 separate patients in the United Kingdom in September raised media and political attention on an emerging public health threat. Nigeria, whose last confirmed case of monkeypox was in 1978, is currently experiencing an unusually large and outbreak of human monkeypox cases, a 'One Human-Environmental-Animal Health' approach is being effectively used to define and tackle the outbreak. As of 13th October 2018, there have been one hundred and sixteen confirmed cases the majority of whom are under 40 years. Read More

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November 2018
2 Reads

Vaccinating against monkeypox in the Democratic Republic of the Congo.

Antiviral Res 2018 Nov 13. Epub 2018 Nov 13.

U.S. Centers for Disease Control and Prevention, Atlanta, USA.

Healthcare-associated transmission of monkeypox has been observed on multiple occasions in areas where the disease is endemic. Data collected by the US Centers for Disease Control and Prevention (CDC) from an ongoing CDC-supported program of enhanced surveillance in the Tshuapa Province of the Democratic Republic of the Congo, where the annual incidence of human monkeypox is estimated to be 3.5-5/10,000, suggests that there is approximately one healthcare worker infection for every 100 confirmed monkeypox cases. Read More

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November 2018
3 Reads

Synthesis and antiviral activity of camphor-based 1,3-thiazolidin-4-one and thiazole derivatives as -reproduction inhibitors.

Medchemcomm 2018 Oct 19;9(10):1746-1753. Epub 2018 Sep 19.

Novosibirsk Institute of Organic Chemistry , Siberian Branch of the Russian Academy of Sciences , Lavrentjev Avenue 9 , 630090 Novosibirsk , Russia . Email:

The genus belongs to the Poxviridae family and includes variola virus (smallpox), cowpox virus, monkeypox virus and vaccinia virus (VV). Smallpox is considered one of the great epidemic disease scourges in human history. It has currently been eradicated; however, it remains a considerable threat as a biowarfare or bioterrorist weapon. Read More

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October 2018
6 Reads

Studies of nonhuman primates: key sources of data on zoonoses and microbiota.

New Microbes New Infect 2018 Nov 30;26:S104-S108. Epub 2018 Aug 30.

Microbes, Evolution, Phylogeny and Infection (MEФI), UMR Aix-Marseille Université, IRD, APHM, IHU Méditerranée Infection, Marseille, France.

The genetic and morphologic similarities between primates and humans means that much information obtained from primates may be applied to humans, and However, habitat loss, hunting and the continued presence of humans have a negative effect on the biology and behaviour of almost all nonhuman primates. Noninvasive methods such as stool collection are among the safest alternative ways to study the multiple aspects of the biology of primates. Many epidemiologic issues (e. Read More

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November 2018
3 Reads

A review of the monkeypox virus and a recent outbreak of skin rash disease in Nigeria.

J Med Virol 2018 Oct 24. Epub 2018 Oct 24.

Virology, Microbiology and Infectious Diseases Research Group, School of Health Sciences, College of Health Sciences, University of KwaZulu-Natal, Durban, South Africa.

Since the eradication of smallpox approximately 39 years ago, monkeypox virus remains the most pathogenic poxvirus, being mainly restricted to Central and West Africa. Before 1970, there were no reports of human monkeypox in Nigeria, while between 1971 and 1978 there were three cases, with none having been reported thereafter. However, in September 2017, a case of contagious skin rash disease, typical of monkeypox, was observed in an 11-year-old boy from the southern part of the country and confirmed to be associated with the monkeypox virus. Read More

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October 2018
7 Reads

Whole Genome Characterization of (OPV) Abatino, a Zoonotic Virus Representing a Putative Novel Clade of Old World Orthopoxviruses.

Viruses 2018 10 6;10(10). Epub 2018 Oct 6.

National Institute for Infectious Diseases Lazzaro Spallanzani IRCCS, via Portuense 292, 00149 Rome, Italy.

(OPVs) are diffused over the complete Eurasian continent, but previously described strains are mostly from northern Europe, and few infections have been reported from Italy. Here we present the extended genomic characterization of OPV Abatino, a novel OPV isolated in Italy from an infected Tonkean macaque, with zoonotic potential. Phylogenetic analysis based on 102 conserved genes (core gene set) showed that OPV Abatino is most closely related to the Ectromelia virus species (ECTV), although placed on a separate branch of the phylogenetic tree, bringing substantial support to the hypothesis that this strain may be part of a novel OPV clade. Read More

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October 2018
2 Reads

Propagation and Purification of Ectromelia Virus.

Curr Protoc Microbiol 2018 Nov 3;51(1):e65. Epub 2018 Oct 3.

School of Medicine, College of Health and Medicine, University of Tasmania, Hobart, Australia.

Ectromelia virus (ECTV) is an orthopoxvirus that causes mousepox in mice. Members of the genus orthopoxvirus are closely related and include variola (the causative agent of smallpox in humans), monkeypox, and vaccinia. Common features of variola virus and ECTV further include a restricted host range and similar disease progression in their respective hosts. Read More

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November 2018
1 Read

Sixty seconds on . . . monkeypox.

Authors:
Abi Rimmer

BMJ 2018 Oct 2;363:k4132. Epub 2018 Oct 2.

The BMJ.

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October 2018

Monkeypox contacts: a puzzling problem.

Authors:
The Lancet

Lancet 2018 09 20;392(10152):986. Epub 2018 Sep 20.

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September 2018
5 Reads

Two cases of monkeypox imported to the United Kingdom, September 2018.

Euro Surveill 2018 Sep;23(38)

Emerging Infections and Zoonoses Section, National Infection Service, Public Health England, Colindale, London, United Kingdom.

In early September 2018, two cases of monkeypox were reported in the United Kingdom (UK), diagnosed on 7 September in Cornwall (South West England) and 11 September in Blackpool (North West England). The cases were epidemiologically unconnected and had recently travelled to the UK from Nigeria, where monkeypox is currently circulating. We describe the epidemiology and the public health response for the first diagnosed cases outside the African continent since 2003. Read More

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September 2018
2 Reads

Emergence of Monkeypox as the Most Important Orthopoxvirus Infection in Humans.

Front Public Health 2018 4;6:241. Epub 2018 Sep 4.

Laboratory of Clinical Virology, Department of Microbiology & Immunology, Rega Institute for Medical Research, KU Leuven, Leuven, Belgium.

Monkeypox is an emerging zoonotic disease recognized as the most important orthopoxvirus infection in humans in the smallpox post-eradication era. The clinical presentation of monkeypox is similar to the one of smallpox. The case fatality rate of monkeypox (10%) lies between the case fatality rate of variola major (30%) and variola minor (1%). Read More

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September 2018
1 Read

The national and provincial burden of medically attended influenza-associated influenza-like illness and severe acute respiratory illness in the Democratic Republic of Congo, 2013-2015.

Influenza Other Respir Viruses 2018 Nov 6;12(6):695-705. Epub 2018 Sep 6.

Influenza Division, Centers for Disease Control and Prevention, Atlanta, Georgia.

Background: Estimates of influenza-associated outpatient consultations and hospitalizations are severely limited in low- and middle-income countries, especially in Africa.

Methods: We conducted active prospective surveillance for influenza-like illness (ILI) and severe acute respiratory illness (SARI) at 5 healthcare facilities situated in Kinshasa Province during 2013-2015. We tested upper respiratory tract samples for influenza viruses using a reverse transcription-polymerase chain reaction assay. Read More

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November 2018
5 Reads

Effects of Treatment Delay on Efficacy of Tecovirimat Following Lethal Aerosol Monkeypox Virus Challenge in Cynomolgus Macaques.

J Infect Dis 2018 Sep;218(9):1490-1499

SIGA Technologies, Inc, Corvallis, Oregon.

Background: Tecovirimat (ST-246) is being developed as an antiviral therapeutic for smallpox for use in the event of an accidental or intentional release. The last reported case of smallpox was 1978 but the potential for use of variola virus for biowarfare has renewed interest in smallpox antiviral therapeutics.

Methods: Cynomolgus macaques were challenged with a lethal dose of monkeypox virus (MPXV) by aerosol as a model for human smallpox and treated orally with 10 mg/kg tecovirimat once daily starting up to 8 days following challenge. Read More

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September 2018
2 Reads

Oral Tecovirimat for the Treatment of Smallpox.

N Engl J Med 2018 07;379(1):44-53

From SIGA Technologies, Corvallis, OR.

Background: Smallpox was declared eradicated in 1980, but variola virus (VARV), which causes smallpox, still exists. There is no known effective treatment for smallpox; therefore, tecovirimat is being developed as an oral smallpox therapy. Because clinical trials in a context of natural disease are not possible, an alternative developmental path to evaluate efficacy and safety was needed. Read More

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July 2018
3 Reads

A many probes-one spot hybridization oligonucleotide microarray.

Anal Bioanal Chem 2018 Sep 22;410(23):5817-5823. Epub 2018 Jun 22.

Institute of Chemical Biology and Fundamental Medicine, Siberian Branch, Russian Academy of Sciences, pr. Lavrentieva 8, Novosibirsk, 630090, Russia.

A variant of the hybridization oligonucleotide microarray, utilizing the principle of many probes-one spot (MPOS-microarrays), is proposed. A case study based on Orthopoxviruses (Variola, Monkeypox, and Ectromelia viruses) demonstrates a considerable increase in the fluorescence signal (up to 100-fold) when several oligonucleotide probes are printed to one spot. Moreover, the specificity of detection also increases (almost 1000-fold), allowing the use of probes that individually lack such high specificity. Read More

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September 2018
9 Reads

Intranasal monkeypox marmoset model: Prophylactic antibody treatment provides benefit against severe monkeypox virus disease.

PLoS Negl Trop Dis 2018 06 21;12(6):e0006581. Epub 2018 Jun 21.

United States Army Medical Research Institute of Infectious Diseases, Virology Division, Fort Detrick Maryland, United States of America.

Concerns regarding outbreaks of human monkeypox or the potential reintroduction of smallpox into an immunological naïve population have prompted the development of animal models and countermeasures. Here we present a marmoset model of monkeypox and smallpox disease utilizing a relevant poxvirus via a natural exposure route. We found that 1000 plaque forming units (PFU) of Monkeypox virus was sufficient to recapitulate smallpox disease, to include an incubation period of approximately 13 days, followed by the onset of rash, and death between 15 and 17 days. Read More

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June 2018
2 Reads

Strengthening of Surveillance during Monkeypox Outbreak, Republic of the Congo, 2017.

Emerg Infect Dis 2018 06;24(6):1158-1160

Reports of 10 suspected cases of monkeypox in Likouala Department, Republic of the Congo, triggered an investigation and response in March 2017 that included community education and surveillance strengthening. Increasing numbers of outbreaks suggest that monkeypox virus is becoming a more prevalent human pathogen. Diverse approaches are necessary for disease control and prevention. Read More

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June 2018
2 Reads

Genome Sequences of Akhmeta Virus, an Early Divergent Old World Orthopoxvirus.

Viruses 2018 05 12;10(5). Epub 2018 May 12.

Poxvirus and Rabies Branch, Division of High-Consequence Pathogens and Pathology, National Center for Emerging and Zoonotic Infectious Diseases, Centers of Disease Control and Prevention, 1600 Clifton Road NE, Atlanta, GA 30333, USA.

Annotated whole genome sequences of three isolates of the Akhmeta virus (AKMV), a novel species of orthopoxvirus (OPXV), isolated from the Akhmeta and Vani regions of the country Georgia, are presented and discussed. The AKMV genome is similar in genomic content and structure to that of the cowpox virus (CPXV), but a lower sequence identity was found between AKMV and Old World OPXVs than between other known species of Old World OPXVs. Phylogenetic analysis showed that AKMV diverged prior to other Old World OPXV. Read More

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May 2018
7 Reads

Should we be already worried about Monkeypox?

Med Clin (Barc) 2018 Oct 7;151(8):320-322. Epub 2018 May 7.

Facultad de Medicina, Universitat de Girona, Girona, España.

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October 2018

A Nosocomial Outbreak of Human Monkeypox in the Central African Republic.

Open Forum Infect Dis 2017 3;4(4):ofx168. Epub 2017 Nov 3.

Unité Environnement et Risques Infectieux, Cellule d'Intervention Biologique d'Urgence.

An outbreak of familial monkeypox occurred in the Central African Republic in 2015/2016 by 3 transmission modes: familial, health care-related, and transport-related. Ten people (3 children and 7 adults) were infected. Most presented with cutaneous lesions and fever, and 2 children died. Read More

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November 2017
3 Reads

Mutagenic repair of double-stranded DNA breaks in vaccinia virus genomes requires cellular DNA ligase IV activity in the cytosol.

J Gen Virol 2018 Jun 20;99(6):790-804. Epub 2018 Apr 20.

1​Department of Medical Microbiology, University Medical Center Utrecht, Utrecht, The Netherlands.

Poxviruses comprise a group of large dsDNA viruses that include members relevant to human and animal health, such as variola virus, monkeypox virus, cowpox virus and vaccinia virus (VACV). Poxviruses are remarkable for their unique replication cycle, which is restricted to the cytoplasm of infected cells. The independence from the host nucleus requires poxviruses to encode most of the enzymes involved in DNA replication, transcription and processing. Read More

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June 2018
8 Reads

Reemergence of Human Monkeypox in Nigeria, 2017.

Emerg Infect Dis 2018 06 17;24(6):1149-1151. Epub 2018 Jun 17.

In Nigeria, before 2017 the most recent case of human monkeypox had been reported in 1978. By mid-November 2017, a large outbreak caused by the West African clade resulted in 146 suspected cases and 42 laboratory-confirmed cases from 14 states. Although the source is unknown, multiple sources are suspected. Read More

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June 2018
20 Reads

Investigation of an outbreak of monkeypox in an area occupied by armed groups, Central African Republic.

Med Mal Infect 2018 Jun 21;48(4):263-268. Epub 2018 Mar 21.

Institut Pasteur de Bangui, Central African Republic.

Background: Monkeypox is a zoonosis caused by an Orthopoxvirus of the Poxviridae family. Human infections are often severe and are a public health problem.

Patients And Method: We conducted a monkeypox outbreak investigation of suspected case patients in five villages of the Alindao-Mingala Health District following blood sample confirmation of the virus by the Institut Pasteur in Bangui. Read More

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June 2018
2 Reads

Evolution of Synonymous Codon Usage Bias in West African and Central African Strains of Monkeypox Virus.

Evol Bioinform Online 2018 9;14:1176934318761368. Epub 2018 Mar 9.

Department of Biotechnology, Vignan's University, Guntur, India.

The evolution of bias in synonymous codon usage in chosen monkeypox viral genomes and the factors influencing its diversification have not been reported so far. In this study, various trends associated with synonymous codon usage in chosen monkeypox viral genomes were investigated, and the results are reported. Identification of factors that influence codon usage in chosen monkeypox viral genomes was done using various codon usage indices, such as the relative synonymous codon usage, the effective number of codons, and the codon adaptation index. Read More

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March 2018
1 Read

Emergence of monkeypox in West Africa and Central Africa, 1970–2017.

Authors:

Wkly Epidemiol Rec 2018 Mar 16;93(11):125-32. Epub 2018 Mar 16.

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March 2018
2 Reads

Emergence of Monkeypox - West and Central Africa, 1970-2017.

MMWR Morb Mortal Wkly Rep 2018 Mar 16;67(10):306-310. Epub 2018 Mar 16.

The recent apparent increase in human monkeypox cases across a wide geographic area, the potential for further spread, and the lack of reliable surveillance have raised the level of concern for this emerging zoonosis. In November 2017, the World Health Organization (WHO), in collaboration with CDC, hosted an informal consultation on monkeypox with researchers, global health partners, ministries of health, and orthopoxvirus experts to review and discuss human monkeypox in African countries where cases have been recently detected and also identify components of surveillance and response that need improvement. Endemic human monkeypox has been reported from more countries in the past decade than during the previous 40 years. Read More

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March 2018
6 Reads

Loss of Actin-Based Motility Impairs Ectromelia Virus Release In Vitro but Is Not Critical to Spread In Vivo.

Viruses 2018 03 5;10(3). Epub 2018 Mar 5.

School of Life and Environmental Sciences, The University of Sydney, Sydney, NSW 2006, Australia.

Ectromelia virus (ECTV) is an orthopoxvirus and the causative agent of mousepox. Like other poxviruses such as variola virus (agent of smallpox), monkeypox virus and vaccinia virus (the live vaccine for smallpox), ECTV promotes actin-nucleation at the surface of infected cells during virus release. Homologs of the viral protein A36 mediate this function through phosphorylation of one or two tyrosine residues that ultimately recruit the cellular Arp2/3 actin-nucleating complex. Read More

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March 2018
6 Reads

Modulating Vaccinia Virus Immunomodulators to Improve Immunological Memory.

Viruses 2018 02 28;10(3). Epub 2018 Feb 28.

Department of Pathology, University of Cambridge, Tennis Court Road, Cambridge CB2 1QP, UK.

The increasing frequency of monkeypox virus infections, new outbreaks of other zoonotic orthopoxviruses and concern about the re-emergence of smallpox have prompted research into developing antiviral drugs and better vaccines against these viruses. This article considers the genetic engineering of vaccinia virus (VACV) to enhance vaccine immunogenicity and safety. The virulence, immunogenicity and protective efficacy of VACV strains engineered to lack specific immunomodulatory or host range proteins are described. Read More

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February 2018
1 Read

Bayesian reconstruction of the evolutionary history and cross-species transition of variola virus and orthopoxviruses.

J Med Virol 2018 Jun 12;90(6):1134-1141. Epub 2018 Mar 12.

Department of Biomedical and Clinical Sciences "Luigi Sacco", University of Milan, Milan, Italy.

Variola virus (VARV), the causative agent of smallpox, is an exclusively human virus belonging to the genus Orthopoxvirus, which includes many other viral species covering a wide range of mammal hosts, such as vaccinia, cowpox, camelpox, taterapox, ectromelia, and monkeypox virus. The tempo and mode of evolution of Orthopoxviruses were reconstructed using a Bayesian phylodynamic framework by analysing 80 hemagglutinin sequences retrieved from public databases. Bayesian phylogeography was used to estimate their putative ancestral hosts. Read More

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June 2018
9 Reads

Ghosts of infections past: using archival samples to understand a century of monkeypox virus prevalence among host communities across space and time.

R Soc Open Sci 2018 Jan 31;5(1):171089. Epub 2018 Jan 31.

Department of Ecology and Evolutionary Biology, Institute of the Environment and Sustainability, University of California, Los Angeles, CA, USA.

Infectious diseases that originate from multiple wildlife hosts can be complex and problematic to manage. A full understanding is further limited by large temporal and spatial gaps in sampling. However, these limitations can be overcome, in part, by using historical samples, such as those derived from museum collections. Read More

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January 2018
6 Reads

Beyond the Dirty Dozen: A Proposed Methodology for Assessing Future Bioweapon Threats.

Mil Med 2018 01;183(1-2):e59-e65

CBRN Program Office, Office of the U.S. Army Surgeon General, Falls Church, VA 22041.

Background: Defense policy planners and countermeasure developers are often faced with vexing problems involving the prioritization of resources and efforts. This is especially true in the area of Biodefense, where each new emerging infectious disease outbreak brings with it questions regarding the causative agent's potential for weaponization. Recent experience with West Nile Virus, Severe Acute Respiratory Syndrome, Monkeypox, and H1N1 Influenza highlights this problem. Read More

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January 2018
5 Reads

Improving the Care and Treatment of Monkeypox Patients in Low-Resource Settings: Applying Evidence from Contemporary Biomedical and Smallpox Biodefense Research.

Viruses 2017 12 12;9(12). Epub 2017 Dec 12.

US Centers for Disease Control and Prevention, Poxvirus and Rabies Branch, Atlanta, GA 30329, USA.

Monkeypox is a smallpox-like illness that can be accompanied by a range of significant medical complications. To date there are no standard or optimized guidelines for the clinical management of monkeypox (MPX) patients, particularly in low-resource settings. Consequently, patients can experience protracted illness and poor outcomes. Read More

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December 2017
10 Reads

Suppression of Poxvirus Replication by Resveratrol.

Front Microbiol 2017 17;8:2196. Epub 2017 Nov 17.

Division of Biology, Kansas State University, Manhattan, KS, United States.

Poxviruses continue to cause serious diseases even after eradication of the historically deadly infectious human disease, smallpox. Poxviruses are currently being developed as vaccine vectors and cancer therapeutic agents. Resveratrol is a natural polyphenol stilbenoid found in plants that has been shown to inhibit or enhance replication of a number of viruses, but the effect of resveratrol on poxvirus replication is unknown. Read More

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November 2017
11 Reads

Frameworks for Preventing, Detecting, and Controlling Zoonotic Diseases.

Emerg Infect Dis 2017 12;23(13)

Preventing zoonotic diseases requires coordinated actions by government authorities responsible for human and animal health. Constructing the frameworks needed to foster intersectoral collaboration can be approached in many ways. We highlight 3 examples of approaches to implement zoonotic disease prevention and control programs. Read More

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December 2017
50 Reads

Validation of a pan-orthopox real-time PCR assay for the detection and quantification of viral genomes from nonhuman primate blood.

Virol J 2017 11 3;14(1):210. Epub 2017 Nov 3.

Division of Medicine, U.S. Army Medical Research Institute of Infectious Diseases, 1425 Porter Street, Fort Detrick, Frederick, MD, 21702-5011, USA.

Background: In 1980, smallpox disease was eradicated from nature and Variola virus, the etiological agent of smallpox, was confined to two laboratories, one located in Russia (Moscow) later moved to VECTOR (Novosibirsk, Siberia) and one in the United States (CDC Atlanta). Vaccinations among the general public ceased shortly after the successful eradication campaign, resulting in an increasingly immunologically susceptible population. Because of the possibility of intentional reintroduction of Variola virus and the emergence of other pathogenic poxviruses, there is a great need for the development of medical countermeasures to treat poxvirus disease. Read More

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November 2017
15 Reads

Toward Understanding the Outcomes of Monkeypox Infection in Human Pregnancy.

J Infect Dis 2017 Oct;216(7):795-797

Donald P. Shiley BioScience Center.

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October 2017
9 Reads

Maternal and Fetal Outcomes Among Pregnant Women With Human Monkeypox Infection in the Democratic Republic of Congo.

J Infect Dis 2017 Oct;216(7):824-828

L'Institut National de Recherche Biomédicale.

Human monkeypox is an endemic disease in rain-forested regions of central Democratic Republic of Congo. We report fetal outcomes for 1 of 4 pregnant women who participated in an observational study at the General Hospital of Kole (Sankuru Province), where 222 symptomatic subjects were followed between 2007 and 2011. Of the 4 pregnant women, 1 gave birth to a healthy infant, 2 had miscarriages in the first trimester, and 1 had fetal death, with the macerated stillborn showing diffuse cutaneous maculopapillary skin lesions involving the head, trunk and extremities, including palms of hands and soles of feet. Read More

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October 2017
53 Reads

Assessing Monkeypox Virus Prevalence in Small Mammals at the Human-Animal Interface in the Democratic Republic of the Congo.

Viruses 2017 10 3;9(10). Epub 2017 Oct 3.

U.S. Centers for Disease Control and Prevention, Poxvirus and Rabies Branch, 1600 Clifton Rd. NE, Atlanta, GA 30333, USA.

During 2012, 2013 and 2015, we collected small mammals within 25 km of the town of Boende in Tshuapa Province, the Democratic Republic of the Congo. The prevalence of monkeypox virus (MPXV) in this area is unknown; however, cases of human infection were previously confirmed near these collection sites. Samples were collected from 353 mammals (rodents, shrews, pangolins, elephant shrews, a potamogale, and a hyrax). Read More

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October 2017
19 Reads

Varicella Coinfection in Patients with Active Monkeypox in the Democratic Republic of the Congo.

Ecohealth 2017 09 11;14(3):564-574. Epub 2017 Sep 11.

UCLA Fielding School of Public Health, 41-275 CHS, 650 Charles E. Young Dr. South, Los Angeles, CA, 90095, USA.

From 2006 to 2007, an active surveillance program for human monkeypox (MPX) in the Democratic Republic of the Congo identified 151 cases of coinfection with monkeypox virus and varicella zoster virus from 1158 suspected cases of human MPX (13%). Using clinical and socio-demographic data collected with standardized instruments by trained, local nurse supervisors, we examined a variety of hypotheses to explain the unexpectedly high proportion of coinfections among the sample, including the hypothesis that the two viruses occur independently. The probabilities of disease incidence and selection necessary to yield the observed sample proportion of coinfections under an assumption of independence are plausible given what is known and assumed about human MPX incidence. Read More

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September 2017
18 Reads

Enhancing case definitions for surveillance of human monkeypox in the Democratic Republic of Congo.

PLoS Negl Trop Dis 2017 Sep 11;11(9):e0005857. Epub 2017 Sep 11.

Poxvirus and Rabies Branch, Division of High-Consequence Pathogens and Pathology, National Center for Emerging and Zoonotic Infectious Diseases, U.S. Centers for Disease Control and Prevention, Atlanta, Georgia, United States of America.

Background: Human monkeypox (MPX) occurs at appreciable rates in the Democratic Republic of Congo (DRC). Infection with varicella zoster virus (VZV) has a similar presentation to that of MPX, and in areas where MPX is endemic these two illnesses are commonly mistaken. This study evaluated the diagnostic utility of two surveillance case definitions for MPX and specific clinical characteristics associated with laboratory-confirmed MPX cases. Read More

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September 2017
20 Reads

Characterization of Monkeypox virus infection in African rope squirrels (Funisciurus sp.).

PLoS Negl Trop Dis 2017 Aug 21;11(8):e0005809. Epub 2017 Aug 21.

US Geological Survey, National Wildlife Health Center, Madison, Wisconsin, United States of America.

Monkeypox (MPX) is a zoonotic disease endemic in Central and West Africa and is caused by Monkeypox virus (MPXV), the most virulent Orthopoxvirus affecting humans since the eradication of Variola virus (VARV). Many aspects of the MPXV transmission cycle, including the natural host of the virus, remain unknown. African rope squirrels (Funisciurus spp. Read More

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August 2017
17 Reads

[F]-Fluorodeoxyglucose Uptake in Lymphoid Tissue Serves as a Predictor of Disease Outcome in the Nonhuman Primate Model of Monkeypox Virus Infection.

J Virol 2017 11 13;91(21). Epub 2017 Oct 13.

Integrated Research Facility, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Frederick, Maryland, USA.

Real-time bioimaging of infectious disease processes may aid countermeasure development and lead to an improved understanding of pathogenesis. However, few studies have identified biomarkers for monitoring infections using imaging. Previously, we demonstrated that positron emission tomography/computed tomography (PET/CT) imaging with [F]-fluorodeoxyglucose (FDG) can monitor monkeypox disease progression in nonhuman primates (NHPs). Read More

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November 2017
26 Reads

Evaluation of Taterapox Virus in Small Animals.

Viruses 2017 08 1;9(8). Epub 2017 Aug 1.

Department of Molecular Microbiology and Immunology, Saint Louis University School of Medicine, 1100 South Grand Boulevard, St. Louis, MO 63104, USA.

Taterapox virus (TATV), which was isolated from an African gerbil () in 1975, is the most closely related virus to variola; however, only the original report has examined its virology. We have evaluated the tropism of TATV in vivo in small animals. We found that TATV does not infect , a species of African dormouse, but does induce seroconversion in the Mongolian gerbil () and in mice; however, in wild-type mice and gerbils, the virus produces an unapparent infection. Read More

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August 2017
7 Reads

Insufficient Innate Immunity Contributes to the Susceptibility of the Castaneous Mouse to Orthopoxvirus Infection.

J Virol 2017 10 12;91(19). Epub 2017 Sep 12.

Laboratory of Viral Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland, USA

The castaneous (CAST) mouse, a wild-derived inbred strain, is highly susceptible to orthopoxvirus infection by intranasal and systemic routes. The 50% lethal intraperitoneal dose of vaccinia virus (VACV) was 3 PFU for CAST mice, whereas BALB/c mice survived 10 PFU. At all times and in all organs analyzed, virus titers were higher in CAST than in BALB/c mice. Read More

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October 2017
17 Reads

Vaccinia virus and Cowpox virus are not susceptible to the interferon-induced antiviral protein MxA.

PLoS One 2017 20;12(7):e0181459. Epub 2017 Jul 20.

Departamento de Biotecnología, Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (I.N.I.A.), Madrid, Spain.

MxA protein is expressed in response to type I and type III Interferon and constitute an important antiviral factor with broad antiviral activity to diverse RNA viruses. In addition, some studies expand the range of MxA antiviral activity to include particular DNA viruses like Monkeypox virus (MPXV) and African Swine Fever virus (ASFV). However, a broad profile of activity of MxA to large DNA viruses has not been established to date. Read More

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September 2017
35 Reads

Comparative Efficacy of Intramuscular and Scarification Routes of Administration of Live Smallpox Vaccine in a Murine Challenge Model.

Vaccine 2017 07 9;35(31):3889-3896. Epub 2017 Jun 9.

CBR Division, Dstl Porton Down, Salisbury SP4 0JQ, UK; The Pirbright Institute, Ash Road, Woking GU24 0NF, UK. Electronic address:

In recent years concern has mounted regarding the possibility of a re-emergence of smallpox through biowarfare or bioterrorism. There is also concern over the incidence of human monkeypox in endemic areas and the potential for monkeypox to be accidentally transported to non-endemic areas. In the event of re-emergence of smallpox or emergence of monkeypox, the accepted route of administration for live replicating smallpox vaccine is dermal scarification, which generates a virus-shedding lesion that persists for several days at the vaccination site. Read More

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July 2017
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