9,994 results match your criteria Molecular medicine reports[Journal]


Anti‑inflammatory effects of Dendropanax morbifera in lipopolysaccharide‑stimulated RAW264.7 macrophages and in an animal model of atopic dermatitis.

Mol Med Rep 2019 Jan 22. Epub 2019 Jan 22.

Department of Companion and Laboratory Animal Science, Kongju National University, Yesan, Chungcheongnam 32439, Republic of Korea.

Dendropanax morbifera (D. morbifera), known as Dendro, means 'omnipotent drug' (Panax), and has been called the panacea tree. Various studies on D. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.3892/mmr.2019.9887DOI Listing
January 2019

Long noncoding RNA GAS5 promotes apoptosis in primary nucleus pulposus cells derived from the human intervertebral disc via Bcl‑2 downregulation and caspase‑3 upregulation.

Mol Med Rep 2019 Jan 22. Epub 2019 Jan 22.

Department of Orthopaedic Surgery, Renji Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200127, P.R. China.

Nucleus pulposus cell (NPC) apoptosis serves an important role in intervertebral disc degeneration (IDD); however, the roles of long noncoding RNAs (lncRNAs) in this process remain unknown. The present study aimed to determine the effects of the lncRNA growth arrest‑specific transcript 5 (GAS5) on the apoptosis of primary human NPCs derived from the intervertebral disc, and to investigate the underlying mechanisms. TargetScan was used to predict the lncRNAs targeted by microRNA‑155 (miR‑155). Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.3892/mmr.2019.9883DOI Listing
January 2019
1 Read
1.484 Impact Factor

miRNA‑135a regulates Hut78 cell proliferation via the GATA‑3/TOX signaling pathway.

Mol Med Rep 2019 Jan 22. Epub 2019 Jan 22.

Department of Dermatology, The First Hospital of Zibo City, Zibo, Shandong 255200, P.R. China.

The present study investigated the role of microRNA‑135a (miR‑135a) in cutaneous T‑cell lymphoma (CTCL) proliferation. Compared with the normal T lymphocyte control cell line, the mRNA and protein levels of GATA binding protein 3 (GATA‑3) were markedly increased in the Hut78 cell line and miR‑135a was markedly decreased (P<0.05). Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.3892/mmr.2019.9885DOI Listing
January 2019

IL‑6 prevents CXCL8‑induced stimulation of EpCAM expression in ovarian cancer cells.

Mol Med Rep 2019 Jan 23. Epub 2019 Jan 23.

Department of Medical Biology and Translational Research, Faculty of Medicine, University of Information Technology and Management, 35‑225 Rzeszow, Poland.

Epithelial cell adhesion molecule (EpCAM), which is expressed in the majority of epithelial tissues, exhibits tumor growth promoting abilities and is overexpressed in human epithelial ovarian cancer. Therefore, EpCAM is considered to be a promising target for specific immune‑based therapies. The present study evaluated the role of IL‑6 and IL‑8 in the expression of EpCAM in the A2780 human ovarian cancer cell line. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.3892/mmr.2019.9890DOI Listing
January 2019

Endothelial progenitor cell impairment mediated vasodilation dysfunction via diminishing nitric oxide production in postmenopausal females.

Mol Med Rep 2019 Jan 23. Epub 2019 Jan 23.

Department of Geriatric Medicine, Xiangya Hospital, Central South University, Changsha, Hunan 410078, P.R. China.

Vascular endothelial dysfunction is the major contributing factor to hypertension. Endothelial progenitor cells (EPCs) are essential for endogenous vascular endothelial renovation. The activity and number of circulating EPCs are preserved in prehypertensive premenopausal females according to our previous research. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.3892/mmr.2019.9888DOI Listing
January 2019
1 Read

NLRP3 inflammasome inactivation driven by miR‑223‑3p reduces tumor growth and increases anticancer immunity in breast cancer.

Mol Med Rep 2019 Jan 23. Epub 2019 Jan 23.

Department of Breast Disease, Beijing Tiantan Hospital, Capital Medical University, Beijing 100050, P.R. China.

MicroRNA‑233‑3p (miR‑223‑3p) is considered an important cancer‑associated marker. The NACHT, LRR and PYD domains‑containing protein 3 (NLRP3) inflammasome represents a novel potential target for the treatment of breast cancer. Therefore, it was hypothesized that miR‑223‑3p may affect tumor growth and immunosuppression in breast cancer by inhibiting the NLRP3 inflammasome. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.3892/mmr.2019.9889DOI Listing
January 2019
1 Read

Expression and significance of EBV, ARID1A and PIK3CA in gastric carcinoma.

Mol Med Rep 2019 Jan 22. Epub 2019 Jan 22.

Department of Pathology, Central South University, Xiangya School of Medicine, Affiliated Haikou Hospital, Haikou, Hainan 570208, P.R. China.

AT‑rich interaction domain 1A (ARID1A) and phosphatidylinositol‑4,5‑bisphosphate 3‑kinase catalytic subunit α (PIK3CA) serve important roles in the formation and development of numerous malignancies including gastric cancer. Accumulating evidence has demonstrated that Epstein‑Barr virus (EBV) is a pathogenic virus associated with gastric cancer. The present study aimed to investigate the association between EBV infection, and the expression levels of ARID1A and PIK3CA in gastric cancer. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.3892/mmr.2019.9886DOI Listing
January 2019
1 Read

Identification of critical genes associated with spinal cord injury based on the gene expression profile of spinal cord tissues from trkB.T1 knockout mice.

Mol Med Rep 2019 Jan 22. Epub 2019 Jan 22.

Department of Rehabilitation, Shanghai General Hospital, Shanghai Jiao Tong University, Shanghai 200080, P.R. China.

The present study aimed to identify the genes and underlying mechanisms critical to the pathology of spinal cord injury (SCI). Gene expression profiles of spinal cord tissues of trkB.T1 knockout (KO) mice following SCI were accessible from the Gene Expression Omnibus database. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.3892/mmr.2019.9884DOI Listing
January 2019
1.484 Impact Factor

Establishment and evaluation of a simulated high‑altitude hypoxic brain injury model in SD rats.

Mol Med Rep 2019 Feb 5. Epub 2019 Feb 5.

Department of Pharmacology of Chinese Materia Medica, School of Pharmacy, Chengdu University of Traditional Chinese Medicine, Wenjiang, Chengdu, Sichuan 611137, P.R. China.

This study was conducted to establish a stable hypobaric hypoxia brain injury model. SD rats were randomly separated into control and model groups, and placed outside or inside of a hypobaric chamber, respectively. Subsequent to 24 h anoxic exposure, plasma superoxide dismutase (SOD), malondialdehyde (MDA), glutathione (GSH), oxidized glutathione (GSSG) and lactate dehydrogenase (LDH) were measured using commercial biochemical kits. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.3892/mmr.2019.9939DOI Listing
February 2019
2 Reads
1.484 Impact Factor

Oxidized unsaturated fatty acids induce apoptotic cell death in cultured cells.

Mol Med Rep 2019 Feb 5. Epub 2019 Feb 5.

Department of Materials and Life Science, Faculty of Science and Technology, Seikei University, Tokyo 180‑8633, Japan.

Polyunsaturated fatty acids are oxidized by non‑enzymatic or enzymatic reactions. The oxidized products are multifunctional. In this study, we investigated how oxidized fatty acids inhibit cell proliferation in cultured cells. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.3892/mmr.2019.9940DOI Listing
February 2019

Inflammatory and fibrosis infiltration in synovium associated with the progression in developmental dysplasia of the hip.

Mol Med Rep 2019 Jan 30. Epub 2019 Jan 30.

Department of Orthopedic Surgery, Xin‑Hua Hospital, Shanghai Jiao‑Tong University School of Medicine, Shanghai 200092, P.R. China.

Developmental dysplasia of the hip (DDH) is a common musculoskeletal disorder characterized by progressive joint soreness and limited mobility. The aim of the present study was to investigate the pathological changes and inflammatory infiltration in the hypertrophic synovium of the hip joint associated with the progression of DDH. Synovial biopsies in the hip joint are obtained from patients with moderate DDH and severe DDH during surgery. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.3892/mmr.2019.9910DOI Listing
January 2019

Ketamine‑induced bladder dysfunction is associated with extracellular matrix accumulation and impairment of calcium signaling in a mouse model.

Mol Med Rep 2019 Jan 29. Epub 2019 Jan 29.

Department of Microbiology, Immunology and Biopharmaceuticals, National Chiayi University, Chiayi 600, Taiwan, R.O.C.

Due to the rising abuse of ketamine usage in recent years, ketamine‑induced urinary tract syndrome has received increasing attention. The present study aimed to investigate the molecular mechanism underlying ketamine‑associated cystitis in a mouse model. Female C57BL/6 mice were randomly divided into two groups: One group was treated with ketamine (100 mg/kg/day of ketamine for 20 weeks), whereas, the control group was treated with saline solution. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.3892/mmr.2019.9907DOI Listing
January 2019

Rhynchophylline ameliorates myocardial ischemia/reperfusion injury through the modulation of mitochondrial mechanisms to mediate myocardial apoptosis.

Mol Med Rep 2019 Jan 30. Epub 2019 Jan 30.

Emergency Department, The Affiliated Hospital of Qingdao University, Qingdao, Shandong 266003, P.R. China.

Rhynchophylline (RP), the primary active ingredient of Uncaria rhynchophylla, has an anti‑hypertensive effect and protects against ischemia‑induced neuronal damage. The present study aimed to examine the roles and mechanisms of RP in myocardial ischemia‑reperfusion (MI/R) injury of rat cardiomyocytes. Cell viability, reactive oxygen species, mitochondrial membrane potential (MMP) and cell apoptosis were examined by a Cell Counting Kit‑8 assay and flow cytometry, respectively. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.3892/mmr.2019.9908DOI Listing
January 2019
2 Reads

Isaindigotone as an inhibitor of the lipopolysaccharide‑induced inflammatory reaction of BV‑2 cells and corresponding mechanisms.

Authors:
Hui Xiao Jianhua Xu

Mol Med Rep 2019 Jan 30. Epub 2019 Jan 30.

Department of Rheumatology and Immunology, The First Affiliated Hospital of Anhui Medical University, Hefei, Anhui 230022, P.R. China.

Isaindigotone possesses extensive pharmacological activities, including anti‑inflammatory effects. The present study investigated the role of isaindigotone in the inhibition of neuroinflammation. Mouse BV‑2 cells were incubated with lipopolysaccharide (LPS; 1 mg/l) for 24 h in a microglial inflammatory model in vitro. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.3892/mmr.2019.9909DOI Listing
January 2019
1.484 Impact Factor

Colonic M1 macrophage is associated with the prolongation of gastrointestinal motility and obesity in mice treated with vancomycin.

Mol Med Rep 2019 Jan 31. Epub 2019 Jan 31.

Division of Gastroenterology, Department of Internal Medicine, Hyogo College of Medicine, Nishinomiya, Hyogo 663‑8501, Japan.

Gut microbiota plays a pivotal role in not only the gastrointestinal (GI) immune system but also GI motility and metabolism. Antibiotic treatments are likely to affect the gut flora and GI immune system, subsequently disturbing GI motility and body metabolism. In the present study, we investigated antibiotic‑induced alterations of body metabolism and GI motility in association with the macrophage profile in the colon. Read More

View Article

Download full-text PDF

Source
http://www.spandidos-publications.com/10.3892/mmr.2019.9920
Publisher Site
http://dx.doi.org/10.3892/mmr.2019.9920DOI Listing
January 2019
1 Read

Comparison of adipose‑ and bone marrow‑derived stem cells in protecting against ox‑LDL‑induced inflammation in M1‑macrophage‑derived foam cells.

Mol Med Rep 2019 Feb 1. Epub 2019 Feb 1.

Division of Cardiovascular Surgery, Second Affiliated Hospital of Harbin Medical University, Key Laboratory of Education Ministry for Myocardial Ischemia, Harbin, Heilongjiang 150086, P.R. China.

Adipose‑derived stem cells (ADSCs) and bone marrow‑derived stem cells (BMSCs) are considered to be prospective sources of mesenchymal stromal cells (MSCs), that can be used in cell therapy for atherosclerosis. The present study investigated whether ADSCs co‑cultured with M1 foam macrophages via treatment with oxidized low‑density lipoprotein (ox‑LDL) would lead to similar or improved anti‑inflammatory effects compared with BMSCs. ADSCs, peripheral blood monocytes, BMSCs and ox‑LDL were isolated from ten coronary heart disease (CHD) patients. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.3892/mmr.2019.9922DOI Listing
February 2019
1 Read

NLRP3/IL1β inflammasome associated with the aging bladder triggers bladder dysfunction in female rats.

Mol Med Rep 2019 Jan 31. Epub 2019 Jan 31.

Department of Urology, Affiliated Hospital of Zunyi Medical University, Zunyi, Guizhou 563003, P.R. China.

Bladder dysfunction is associated with fibrosis‑-mediated aging, but the corresponding mechanism remains to be elucidated. Activation of the NACHT, LRR and PYD domains‑containing protein 3 (NLRP3) inflammasome is related to chronic diseases associated with aging, including organ fibrosis. The present study aimed to explore the role of NLRP3/interleukin 1β in aging‑associated bladder dysfunction. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.3892/mmr.2019.9919DOI Listing
January 2019

Identification of a pathogenic mutation in a Chinese pedigree with polycystic kidney disease.

Mol Med Rep 2019 Jan 31. Epub 2019 Jan 31.

Laboratory of Medical Genetics, Harbin Medical University, Harbin, Heilongjiang 150081, P.R. China.

Polycystic kidney disease (PKD) is a life‑threatening inherited disease with a morbidity of 1:500‑1,000 worldwide. Numerous progressively enlarging cysts are observed in the bilateral kidneys of patients with PKD, inducing structural damage and loss of kidney function. The present study analyzed one family with PKD. Read More

View Article

Download full-text PDF

Source
http://www.spandidos-publications.com/10.3892/mmr.2019.9921
Publisher Site
http://dx.doi.org/10.3892/mmr.2019.9921DOI Listing
January 2019
3 Reads

MicroRNA‑663b targets GAB2 to restrict cell proliferation and invasion in hepatocellular carcinoma.

Mol Med Rep 2019 Feb 5. Epub 2019 Feb 5.

Department of Preventive Medicine, Medical College of Yan'an University, Yan'an, Shanxi 716000, P.R. China.

Previous studies have demonstrated that numerous tumor‑specific microRNAs (miRNAs) are upregulated or downregulated in hepatocellular carcinoma (HCC), and that their dysregulation is implicated in HCC occurrence and development. Therefore, investigation of crucial miRNAs involved in HCC oncogenesis and progression may provide novel insights into the therapy of patients with this malignant tumor. In the present study, reverse transcription‑quantitative polymerase chain reaction (RT‑qPCR) assays were performed to detect tissue and cellular expression levels of miRNA‑663b (miR‑663b) in HCC. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.3892/mmr.2019.9934DOI Listing
February 2019
1 Read

miR‑155 mediates inflammatory injury of hippocampal neuronal cells via the activation of microglia.

Mol Med Rep 2019 Jan 31. Epub 2019 Jan 31.

Institute of Hematology, The First Affiliated Hospital of Zhejiang Chinese Medical University, Hangzhou, Zhejiang 310006, P.R. China.

MicroRNA (miR)‑155 has a crucial role in various cellular functions, including differentiation of hematopoietic cells, immunization, inflammation and cardiovascular diseases. The present study aimed to investigate the roles and mechanisms of miR‑155 in treatment‑resistant depression (TRD). A Cell Counting Kit‑8 assay and flow cytometry were performed to assess the cell viability and apoptosis of microglial cells, respectively. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.3892/mmr.2019.9917DOI Listing
January 2019

MT1‑MMP promotes the proliferation and invasion of gastric carcinoma cells via regulating vimentin and E‑cadherin.

Mol Med Rep 2019 Jan 31. Epub 2019 Jan 31.

Department of Radiotherapy, The First Affiliated Hospital of Suzhou University, Suzhou, Jiangsu 215006, P.R. China.

The present study aimed to explore the possible effects of membrane‑type 1 matrix metalloproteinase (MT1‑MMP) on gastric carcinoma cells proliferation and invasion. Immunohistochemistry analysis was conducted to measure MT1‑MMP expression level in 15 patients with gastric carcinoma. Subsequently, recombinant short hairpin RNA (shRNA) vectors targeting MT1‑MMP were constructed to silence the expression of MT1‑MMP in gastric carcinoma cells. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.3892/mmr.2019.9918DOI Listing
January 2019

[Retracted] Pathogenetic mechanism of lipoid proteinosis caused by mutation of the extracellular matrix protein 1 gene.

Mol Med Rep 2019 Feb 1. Epub 2019 Feb 1.

Department of Laboratory and Oncology, Yantai Yuhuangding Hospital, Yantai, Shandong 264000, P.R. China.

After the publication of the above article, the Editor of Molecular Medicine Reports received an accusation that this paper contained material taken from the PhD thesis of Dr Xiao Bai of the China Academy of Medical Science & Peking Union Medical College, whose thesis entitled "Study on mutations of ECM1 in lipoid proteinosis" was published online in 2015. After having enquired with the authors regarding this allegation, the first author and the corresponding author, Dr Dong Gao, admitted that she had infringed the rights of Dr Bai, and that it was not appropriate that the following figures were published in the research article: Figs. 1, 2, 4, 5, 6 and 7, which coincided with Figs. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.3892/mmr.2019.9925DOI Listing
February 2019

PGAM1 knockdown is associated with busulfan‑induced hypospermatogenesis and spermatogenic cell apoptosis.

Mol Med Rep 2019 Feb 4. Epub 2019 Feb 4.

Center for Reproductive Medicine, Guangdong Armed Police Hospital, Guangzhou Medical University, Guangzhou, Guangdong 510507, P.R. China.

Phosphoglycerate mutase 1 (PGAM1) is reported to be involved in spermatogenic dysfunction. However, the association between PGAM1 and busulfan‑induced hypospermatogenesis and spermatogenic cell apoptosis remains unclear. The aim of the current study was to investigate the association between PGAM1 expression and busulfan‑induced hypospermatogenesis, and the effect of PGAM1 expression on spermatogenic cell apoptosis. Read More

View Article

Download full-text PDF

Source
http://www.spandidos-publications.com/10.3892/mmr.2019.9930
Publisher Site
http://dx.doi.org/10.3892/mmr.2019.9930DOI Listing
February 2019
2 Reads

Fluoxetine induces direct inhibitory effects on mesenchymal stem cell‑derived osteoprogenitor cells independent of serotonin concentration.

Mol Med Rep 2019 Feb 1. Epub 2019 Feb 1.

Urology and Nephrology Center, Faculty of Medicine, Mansoura University, Mansoura 35516, Egypt.

Selective serotonin reuptake inhibitors are the most commonly prescribed antidepressants worldwide, which have been reported to exert potential detrimental effects on bone mineral density and increase the risk of developing fractures. The present study aimed to investigate the pathways underlying the negative effects of fluoxetine on bone using mesenchymal stem cells (MSCs) derived from rat adipose tissue as a source of osteoprogenitor cells. MSCs were harvested from adipose tissue using a collagenase enzyme digestion method and were allowed to differentiate into osteoprogenitor cells. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.3892/mmr.2019.9924DOI Listing
February 2019
1.484 Impact Factor

Implantation of engineered conduction tissue in the rat heart.

Mol Med Rep 2019 Feb 5. Epub 2019 Feb 5.

Institute of Biomedical Engineering, Second Military Medical University, Shanghai 200433, P.R. China.

Engineered conduction tissues (ECTs) are cardiac conduction tissues fabricated in vitro to allow for more precisely targeted in vivo transplantation therapy. The transplantation of ECTs may be ideal for the treatment of atrioventricular conduction block and could have a significant impact on the future application of biological pacemakers. However, there is little published information regarding the conduction function of ECTs in vivo. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.3892/mmr.2019.9933DOI Listing
February 2019
1 Read

Prediction and analysis of weighted genes in hepatocellular carcinoma using bioinformatics analysis.

Mol Med Rep 2019 Feb 4. Epub 2019 Feb 4.

Department of Hepatobiliary Surgery, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong 510515, P.R. China.

The aim of the present study was to identify the differentially expressed genes (DEGs) between primary tumor tissue and adjacent non‑tumor tissue of hepatocellular carcinoma (HCC) samples in order to investigate the mechanisms of HCC. The microarray data of the datasets GSE76427, GSE84005 and GSE57957 were downloaded from the Gene Expression Omnibus database. DEGs were identified using the limma package in the R programming language. Read More

View Article

Download full-text PDF

Source
http://www.spandidos-publications.com/10.3892/mmr.2019.9929
Publisher Site
http://dx.doi.org/10.3892/mmr.2019.9929DOI Listing
February 2019
1 Read

Tetramethylpyrazine reduces inflammation in the livers of mice fed a high fat diet.

Mol Med Rep 2019 Feb 1. Epub 2019 Feb 1.

Key Laboratory of Geriatrics, Beijing Hospital, National Center of Gerontology, Beijing 100730, P.R. China.

The present study aimed to assess the protective effects of tetramethylpyrazine (TMP) on the livers of mice fed a high fat diet. The mice were divided into five groups: Regular diet; high fat diet; simvastatin‑treated; and low and high dose TMP‑treated groups. The results demonstrated that, compared with the control group, serum glucose, total cholesterol (TC) and low‑density lipoprotein cholesterol levels were increased in the model group. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.3892/mmr.2019.9928DOI Listing
February 2019

Differences in the distribution of CD20, CD3, CD34 and CD45RO in nasal mucosa and polyps from patients with chronic rhinosinusitis.

Mol Med Rep 2019 Feb 5. Epub 2019 Feb 5.

Department of Clinical Pharmacy, University of Medicine and Pharmacy of Craiova, 200349 Craiova, Romania.

The present study focused on the assessment of the inflammatory infiltrate that characterizes nasal polyps in patients with chronic rhinosinusitis and nasal polyposis. Inflammatory cell type was determined using specific markers. This evaluation was made possible by determining the expression of the following markers: CD20, a marker of B lymphocytes [using activated T cells (ATC) armed with CD20 antibody]; CD3, a marker of T lymphocytes (using ATC armed with anti‑CD3 antibody); CD45, the leukocyte common antigen (using ATC armed with anti‑CD45 antibody; and CD34, for the microvasculature of the nasal polyp (using anti‑CD34 antibody). Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.3892/mmr.2019.9932DOI Listing
February 2019

Comprehensive bioinformatics analysis of critical lncRNAs, mRNAs and miRNAs in non‑alcoholic fatty liver disease.

Mol Med Rep 2019 Feb 5. Epub 2019 Feb 5.

Department of General Practice, Nanjing First Hospital, Nanjing Medical University, Nanjing, Jiangsu 210006, P.R. China.

Non‑alcoholic fatty liver disease (NAFLD) is the most common fatty liver disease in developed countries, in which fat accumulation in the liver is induced by non‑alcoholic factors. The present study was conducted to identify NAFLD‑associated long non‑coding RNAs (lncRNAs), mRNAs and microRNAs (miRNAs). The microarray dataset GSE72756, which included 5 NAFLD liver tissues and 5 controls, was acquired from the Gene Expression Omnibus database. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.3892/mmr.2019.9931DOI Listing
February 2019

10‑Hydroxycamptothecin induces apoptosis in human fibroblasts by regulating miRNA‑23b‑3p expression.

Mol Med Rep 2019 Feb 1. Epub 2019 Feb 1.

Department of Orthopedics, The Second Affiliated Hospital of Xiangya School of Medicine, Central South University, Changsha, Hunan 410012, P.R. China.

10‑Hydroxycamptothecin (HCPT) effectively controls epidural fibrosis, but the exact underlying mechanisms remain ambiguous. Abnormal microRNA (miR)‑23b‑3p expression has been detected in various types of fibrotic tissues that are present in different diseases. The aim of the present study was to elucidate the mechanisms through which HCPT induces fibroblast apoptosis. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.3892/mmr.2019.9927DOI Listing
February 2019

RNA sequencing discloses the genome‑wide profile of long noncoding RNAs in dilated cardiomyopathy.

Mol Med Rep 2019 Feb 5. Epub 2019 Feb 5.

Department of Cardiology, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, P.R. China.

Dilated cardiomyopathy (DCM) is a common type of non‑ischemic cardiomyopathy, of which the underlying mechanisms have not yet been fully elucidated. Long noncoding RNAs (lncRNAs) have been reported to serve crucial physiological roles in various cardiac diseases. However, the genome‑wide expression profile of lncRNAs remains to be elucidated in DCM. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.3892/mmr.2019.9937DOI Listing
February 2019
2 Reads

Preliminary study on the role of miR‑148a and DNMT1 in the pathogenesis of acute myeloid leukemia.

Mol Med Rep 2019 Jan 30. Epub 2019 Jan 30.

Department of Hematology, The First Hospital, China Medical University, Shenyang, Liaoning 110001, P.R. China.

MicroRNA (miR)‑148a is differentially expressed in numerous malignant tumors and it was identified to regulate tumor growth, cell proliferation, apoptosis, angiogenesis and drug resistance via the regulation of the expression levels of its target genes. However, the biological function of miR‑148a in acute myeloid leukemia (AML) and its molecular mechanisms of action remain unclear. In the present study, the expression levels of miR‑148a and DNA methyltransferase 1 (DNMT1) were detected using reverse transcription‑quantitative polymerase chain reaction (PCR) and western blotting. Read More

View Article

Download full-text PDF

Source
http://www.spandidos-publications.com/10.3892/mmr.2019.9913
Publisher Site
http://dx.doi.org/10.3892/mmr.2019.9913DOI Listing
January 2019
3 Reads

Integrative analysis of cancer driver genes in prostate adenocarcinoma.

Mol Med Rep 2019 Jan 28. Epub 2019 Jan 28.

Department of Urology, The Affiliated Hospital of Southwest Medical University, Luzhou, Sichuan 646000, P.R. China.

Large‑scale genomics studies have identified recurrently mutated genes in the ETS gene family, including fusions and copy number variations (CNVs), which are involved in the development of prostate adenocarcinoma (PRAD). However, the aetiology of PRAD remains to be fully elucidated. In the present study, 333 driver genes were identified using four computational tools: OncodriveFM, OncodriveCLUST, iCAGES and DrGaP. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.3892/mmr.2019.9902DOI Listing
January 2019

Comprehensive circular RNA expression profiles in a mouse model of nonalcoholic steatohepatitis.

Mol Med Rep 2019 Feb 5. Epub 2019 Feb 5.

Central Laboratory of The Eighth People's Hospital of Shanghai, Shanghai 200235, P.R. China.

Recent studies have revealed that circular RNAs (circRNAs) are involved in the development of various liver diseases. However, the regulatory role of circRNAs in nonalcoholic steatohepatitis (NASH) has not been fully elucidated. In the present study, the circRNA profiles in a NASH mouse model were investigated, and their functions in NASH were predicted using bioinformatics analysis, with the aim of providing novel clues for delineating the mechanisms of action. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.3892/mmr.2019.9935DOI Listing
February 2019

Aberrant expression of sperm‑specific glycolytic enzymes are associated with poor sperm quality.

Mol Med Rep 2019 Feb 1. Epub 2019 Feb 1.

Central Laboratory, The Affiliated Yantai Yuhuangding Hospital of Qingdao University, Yantai, Shandong 264000, P.R. China.

The energy required for normal sperm function is mainly generated by glycolysis and oxidative phosphorylation. Testis‑specific glycolytic enzymes are expressed in germ cells and present in mature spermatozoa. The objective of the present study was to investigate the association between aberrant expression of glycolytic enzymes and human sperm quality. Read More

View Article

Download full-text PDF

Source
http://www.spandidos-publications.com/10.3892/mmr.2019.9926
Publisher Site
http://dx.doi.org/10.3892/mmr.2019.9926DOI Listing
February 2019
3 Reads
1.484 Impact Factor

Puerarin alters the function of monocytes/macrophages and exhibits chondroprotection in mice.

Mol Med Rep 2019 Feb 5. Epub 2019 Feb 5.

Department of Orthopedics, Changzhou Traditional Chinese Medicine Hospital, Changzhou, Jiangsu 213003, P.R. China.

Recent studies have suggested that puerarin may impede osteoclastogenesis and facilitate bone regeneration, in addition to attenuating tissue inflammation. The present study investigated the therapeutic effects of puerarin on inflammatory responses and monocyte recruitment in in vitro and in vivo osteoarthritis (OA) models. Puerarin treatment increased the proliferation of OA chondrocytes, as determined by Cell Counting Kit‑8 assay. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.3892/mmr.2019.9936DOI Listing
February 2019
1 Read

JMJD2A facilitates growth and inhibits apoptosis of cervical cancer cells by downregulating tumor suppressor miR‑491‑5p.

Mol Med Rep 2019 Jan 31. Epub 2019 Jan 31.

Department of Anesthesiology, The First Affiliated Hospital of Zhejiang Chinese Medical University, Hangzhou, Zhejiang 310006, P.R. China.

Cervical cancer remains the second most common malignancy for women worldwide. Jumonji domain containing 2A (JMJD2A), a member of the JmjC domain‑containing family of JMJD2 proteins, is capable of regulating cancer‑associated genes, including genes involved in the cell cycle, proliferation, apoptosis, invasion and metastasis. However, its role in human cervical cancer has yet to be elucidated. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.3892/mmr.2019.9916DOI Listing
January 2019
1 Read

Regulation of Fgf15 expression in the intestine by glucocorticoid receptor.

Mol Med Rep 2019 Jan 30. Epub 2019 Jan 30.

Wadsworth Center, New York State Department of Health, School of Public Health, State University of New York, Albany, NY 12201, USA.

Fibroblast growth factor 15 (FGF15) was previously identified to be highly expressed in the ileum and functions as an endocrine factor to regulate bile acid synthesis in the liver. FGF15 targets its receptor fibroblast growth factor receptor 4 in the liver and serves important roles in energy metabolism, including bile acid homeostasis, glucose metabolism and protein synthesis. The expression of FGF15 is known to be regulated by the transcription factor farnesoid X receptor (FXR). Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.3892/mmr.2019.9915DOI Listing
January 2019

MicroRNA‑504 targets AEG‑1 and inhibits cell proliferation and invasion in retinoblastoma.

Mol Med Rep 2019 Feb 1. Epub 2019 Feb 1.

Department of Ophthalmology, China‑Japan Union Hospital of Jilin University, Changchun, Jilin 130033, P.R. China.

The dysregulation of microRNAs (miRNAs/miRs) has become increasingly recognized as a primary feature of retinoblastoma (RB). Furthermore, miRNAs have been demonstrated to be involved in the occurrence and development of RB. Therefore, it is crucial to investigate the expression profile and roles of miRNAs in RB in order to identify potential therapeutic targets to treat patients with RB. Read More

View Article

Download full-text PDF

Source
http://www.spandidos-publications.com/10.3892/mmr.2019.9923
Publisher Site
http://dx.doi.org/10.3892/mmr.2019.9923DOI Listing
February 2019
2 Reads

Potential urine biomarkers for gestational hypertension and preeclampsia.

Mol Med Rep 2019 Jan 30. Epub 2019 Jan 30.

Department of Pathophysiology, Southern Medical University, Guangzhou, Guangdong 510515, P.R. China.

Differential proteomic technology was used to identify urine proteomic profile of gestational hypertension and preeclampsia. Urine samples were collected from 10 patients with gestational hypertension, 10 patients with mild preeclampsia, 10 patients with severe preeclampsia and 10 normal pregnancies and analyzed by 2‑D difference gel electrophoresis, then matrix assisted laser desorption ionization mass spectrometry was used to identify differential proteins. Subsequently, ELISA was used to verify the content variation of the identified proteins in 200 urine samples. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.3892/mmr.2019.9911DOI Listing
January 2019
2 Reads

The anthelmintic agent oxfendazole inhibits cell growth in non‑small cell lung cancer by suppressing c‑Src activation.

Mol Med Rep 2019 Jan 28. Epub 2019 Jan 28.

Department of Thoracic Surgery, The First Affiliated Hospital of Soochow University, Suzhou, Jiangsu 215006, P.R. China.

The c‑Src protein family of tyrosine kinases are important in the tumorigenesis of many types of tumors, and may be a potential target for antitumor drug discovery. In the present study, immunoblotting was performed to analyze protein expression, CCK‑8 assay was carried out to assess cell viability and cell cycle was analyzed using a flow cytometer. The anthelmintic agent oxfendazole was observed to be a novel c‑Src inhibitor that blocked the activation of c‑Src. Read More

View Article

Download full-text PDF

Source
http://www.spandidos-publications.com/10.3892/mmr.2019.9897
Publisher Site
http://dx.doi.org/10.3892/mmr.2019.9897DOI Listing
January 2019
1 Read

Investigating age‑induced differentially expressed genes and potential molecular mechanisms in osteosarcoma based on integrated bioinformatics analysis.

Mol Med Rep 2019 Jan 30. Epub 2019 Jan 30.

Department of Orthopedics Ward II, Shenzhen Children's Hospital, Shenzhen, Guangdong 518038, P.R. China.

Osteosarcoma (OS) is the most common primary bone malignancy. It predominantly occurs in adolescents, but can develop at any age. The age at diagnosis is a prognostic factor of OS, but the molecular basis of this remains unknown. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.3892/mmr.2019.9912DOI Listing
January 2019

Construction of a cDNA library and preliminary analysis of the expressed sequence tags of the earthworm Eisenia fetida (Savigny, 1826).

Mol Med Rep 2019 Feb 5. Epub 2019 Feb 5.

Department of Biochemistry and Molecular Biology, Shanxi Medical University, Taiyuan, Shanxi 030001, P.R. China.

Earthworms are useful indicator organisms of soil health and Eisenia fetida have been extensively used as test organisms in ecotoxicological studies. In order to gain insight into the gene expression profiles associated with physiological functions of earthworms, a full‑length enriched cDNA library of the Eisenia fetida genome was successfully constructed using Switching Mechanism at 5'End of RNA Template technology. Construction of a cDNA library and analysis of Expressed Sequence Tags (ESTs) are efficient approaches for collecting genomic information and identifying genes important for a given biological process. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.3892/mmr.2019.9938DOI Listing
February 2019
1.484 Impact Factor

Upregulation of ADAMTS‑7 and downregulation of COMP are associated with spontaneous abortion.

Mol Med Rep 2019 Jan 28. Epub 2019 Jan 28.

Reproductive Medicine Center, Renmin Hospital of Wuhan University, Hubei Clinic Research Center for Assisted Reproductive Technology and Embryonic Development, Wuhan University, Wuhan, Hubei 430060, P.R. China.

A disintegrin and metalloproteinase with thrombospondin motifs 7 (ADAMTS‑7) has been revealed to serve an important role in inflammation‑associated diseases. However, the role of ADAMTS‑7 in spontaneous abortion (SA) remains unclear. In the present study, human and mouse decidual tissues were used to detect the expression of ADAMTS‑7 and cartilage oligomeric matrix protein (COMP) in mice with lipopolysaccharide (LPS)‑induced abortion (10 mice/group), and in SA humans and the corresponding control group (21 participants in the SA group and 15 participants in the control group). Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.3892/mmr.2019.9898DOI Listing
January 2019
1 Read

Knockdown of ARK5 expression suppresses invasion of ovarian cancer cells.

Mol Med Rep 2019 Jan 28. Epub 2019 Jan 28.

Department of Pharmacology, Weifang Medical University, Weifang, Shandong 261053, P.R. China.

The aim of the current study was to investigate the effects and the molecular mechanisms of ARK5 in ovarian cancer cell invasion. The plasmid pGCsilencerU6/GFP/Neo‑RNAi‑ARK5 and the control vector with a scramble sequence were transfected into SKOV3 cells to establish ARK5‑deficient SKOV3 cells (siARK5/SKOV3) and a control cell line (Scr/SKOV3), respectively. Reverse transcription‑polymerase chain reaction (RT‑PCR) and Western blot analysis were used to determine the mRNA and protein expression levels of ARK5. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.3892/mmr.2019.9901DOI Listing
January 2019
1 Read
1.484 Impact Factor

Simultaneous detection of target CNVs and SNVs of thalassemia by multiplex PCR and next‑generation sequencing.

Mol Med Rep 2019 Jan 24. Epub 2019 Jan 24.

Institute of Antibody Engineering, School of Laboratory Medicine and Biotechnology, Southern Medical University, Guangzhou, Guangdong 510515, P.R. China.

Thalassemia is caused by complex mechanisms, including copy number variants (CNVs) and single nucleotide variants (SNVs). The CNV types of α‑thalassemia are typically detected by gap‑polymerase chain reaction (PCR). The SNV types are detected by Sanger sequencing. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.3892/mmr.2019.9896DOI Listing
January 2019

BP‑1‑102 exerts an antitumor effect on the AGS human gastric cancer cell line through modulating the STAT3 and MAPK signaling pathways.

Mol Med Rep 2019 Jan 24. Epub 2019 Jan 24.

Cancer Center, The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, Zhejiang 310003, P.R. China.

BP‑1‑102, a novel inhibitor of signal transducer and activator of transcription 3 (STAT3), exhibits significant antitumor effects in several malignancies in vitro and in vivo. However, its role in gastric cancer (GC) remains to be elucidated. In the present study, the effect and potential molecular mechanisms of BP‑102 in human GC cell lines were investigated. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.3892/mmr.2019.9892DOI Listing
January 2019

Expression and role of TNIP2 in multiple organ dysfunction syndrome following severe trauma.

Mol Med Rep 2019 Jan 24. Epub 2019 Jan 24.

Department of Emergency Surgery, Affiliated Hospital of Nantong University, Nantong, Jiangsu 226001, P.R. China.

Severe trauma can result in secondary multiple organ dysfunction syndrome (MODS) and death. Inflammation response and oxidative stress promote the occurrence and development of MODS. TNFAIP3‑interacting protein 2 (TNIP2), which can repress the activation of nuclear factor‑κB (NF‑κB) and may be involved in MODS progression, has not been studied in regards to MODS. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.3892/mmr.2019.9893DOI Listing
January 2019

Functional genetic variants of the GATA4 gene promoter in acute myocardial infarction.

Mol Med Rep 2019 Jan 30. Epub 2019 Jan 30.

Department of Anatomy and Regenerative Biology, The George Washington University, Washington, DC 20037, USA.

Coronary artery disease (CAD), including acute myocardial infarction (AMI), is a common complex disease; however, the genetic causes remain largely unknown. Recent epidemiological investigations indicated that the incidence of CAD in patients with congenital heart diseases is markedly higher than that observed in healthy controls. It was therefore hypothesized that the dysregulated expression of cardiac developmental genes may be involved in CAD development. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.3892/mmr.2019.9914DOI Listing
January 2019
2 Reads

circRNA‑miRNA association for coronary heart disease.

Mol Med Rep 2019 Jan 28. Epub 2019 Jan 28.

Cardiovascular Research Center, The First Affiliated Hospital of Xinxiang Medical University, Xinxiang, Henan 453100, P.R. China.

Coronary heart disease (CHD) is a major cause of morbidity and mortality and an important public health problem globally, but the mechanism of CHD is still complex and unclear. The purpose of the current study was to explore the mechanism underlying CHD using high‑throughput technology. The study participants were patients with coronary angiography (CAG)‑proven severity of coronary artery stenosis. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.3892/mmr.2019.9905DOI Listing
January 2019