2,840,803 results match your criteria Molecular ecology[Journal]


Infection of HeLa cells with Chlamydia trachomatis inhibits protein synthesis and causes multiple changes to host cell pathways.

Cell Microbiol 2018 Dec 14:e12993. Epub 2018 Dec 14.

Institute for Microbiology and Hygiene, Medical Center - University of Freiburg, Faculty of Medicine, University of Freiburg, Germany.

The obligate intracellular bacterium Chlamydia trachomatis replicates in a cytosolic vacuole in human epithelial cells. Infection of human cells with C. trachomatis causes substantial changes to many host cell signalling pathways but the molecular basis of such influence is not well understood. Read More

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December 2018

Children's untreated decay is positively associated with past caries experience and with current salivary loads of mutans Streptococci; negatively with self-reported maternal iron supplements during pregnancy: a multifactorial analysis.

J Public Health Dent 2018 Dec 14. Epub 2018 Dec 14.

Menzies Health Institute Queensland, Griffith University, Gold Coast, Queensland, Australia.

Objectives: This study explored the association of children's salivary characteristics, past caries experience, birth weight, and reported maternal prenatal vitamin and mineral supplementation with the dental untreated decay of the child.

Methods: This cross-sectional study, a sub-study of Griffith University Environments for Healthy Living birth cohort study, was conducted on 174 mother-child dyads. Mother's prenatal usage of vitamin and mineral supplements; child's birthweight; salivary pH, buffering capacity, and levels of salivary MS and LB were explored as risk indicators. Read More

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December 2018

Novel endotypes in heart failure: effects on guideline-directed medical therapy.

Eur Heart J 2018 Dec 13. Epub 2018 Dec 13.

Department of Cardiology, University of Groningen, Hanzeplein 1, Groningen, the Netherlands.

Aims: We sought to determine subtypes of patients with heart failure (HF) with a distinct clinical profile and treatment response, using a wide range of biomarkers from various pathophysiological domains.

Methods And Results: We performed unsupervised cluster analysis using 92 established cardiovascular biomarkers to identify mutually exclusive subgroups (endotypes) of 1802 patients with HF and reduced ejection fraction (HFrEF) from the BIOSTAT-CHF project. We validated our findings in an independent cohort of 813 patients. Read More

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December 2018

Association of Upregulated Angiogenic Cytokines With Choroidal Abnormalities in Chronic Central Serous Chorioretinopathy.

Invest Ophthalmol Vis Sci 2018 Dec;59(15):5924-5931

Department of Ophthalmology, Kyoto Prefectural University of Medicine, Kyoto, Japan.

Purpose: To clarify the distinct molecular pathogenesis of central serous chorioretinopathy (CSC) and pachychoroid neovasculopathy (PNV).

Methods: Aqueous humor (AH) was collected from 18 acute CSC, 20 chronic CSC, and 20 PNV patients. Concentrations of 30 cytokines in the AH were analyzed using a multiplex bead immunoassay, and the cytokine profiles were compared among these three groups of patients. Read More

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December 2018

Calreticulin regulates syncytialization through control of the synthesis and transportation of E-cadherin in BeWo cells.

Endocrinology 2018 Dec 14. Epub 2018 Dec 14.

Department of Obstetrics and Gynecology, Wakayama Medical University.

During placental development, mononuclear cytotrophoblasts differentiate and fuse to syncytiotrophoblasts (STB) to form syncytia, which secrete human chorionic gonadotropin (hCG). Decreased maternal perfusion of the placenta, which leads to placental dysfunction, induces changes in trophoblast syncytialization. Our previous study showed that calreticulin (CRT), a Ca2+-binding molecular chaperone found in the endoplasmic reticulum, is expressed in the human placenta and is involved in regulating extravillous trophoblast invasion, although its role in villous trophoblasts remains unclear. Read More

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December 2018

Work Loss Before and After Diagnosis of Crohn's Disease.

Inflamm Bowel Dis 2018 Dec 14. Epub 2018 Dec 14.

Department of Clinical Science and Education, Södersjukhuset, Karolinska Institutet, Stockholm, Sweden.

Background: The aim of this study was to examine work loss in patients with Crohn's disease.

Methods: Using nationwide registers, we identified incident patients with Crohn's disease (2007-2010) and population comparator subjects without inflammatory bowel disease, matched by age, sex, calendar year, health care region, and education level. We assessed the number of lost workdays due to sick leave and disability pension from 5 years before to 5 years after first diagnosis of Crohn's disease or end of follow-up (September 30, 2015). Read More

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December 2018

In vitro breeding: application of embryonic stem cells to animal production.

Biol Reprod 2018 Dec 14. Epub 2018 Dec 14.

Department of Animal Science, University of California, Davis, CA.

Embryonic stem cells (ESCs) are derived from the inner cell mass of preimplantation blastocysts. For decades, attempts to efficiently derive ESCs in animal livestock species have been unsuccessful, but this goal has recently been achieved in cattle. Together with the recent reconstitution of the germ cell differentiation processes from ESCs in mice, these achievements open new avenues for the development of promising technologies oriented toward improving health, animal production and the environment. Read More

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December 2018

A decision tree-based integrated testing strategy for tailor-made carcinogenicity evaluation of test substances using genotoxicity test results and chemical spaces.

Mutagenesis 2018 Dec 14. Epub 2018 Dec 14.

R&D, Safety Science Research, Kao Corporation, Ichikai-Machi, Haga-Gun, Tochigi, Japan.

Genotoxicity evaluation has been widely used to estimate the carcinogenicity of test substances during safety evaluation. However, the latest strategies using genotoxicity tests give more weight to sensitivity; therefore, their accuracy has been very low. For precise carcinogenicity evaluation, we attempted to establish an integrated testing strategy for the tailor-made carcinogenicity evaluation of test materials, considering the relationships among genotoxicity test results (Ames, in vitro mammalian genotoxicity and in vivo micronucleus), carcinogenicity test results and chemical properties (molecular weight, logKow and 179 organic functional groups). Read More

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December 2018

Cytological and molecular aspects of the ageing sperm.

Hum Reprod 2018 Dec 14. Epub 2018 Dec 14.

Laboratory of Seminology - Sperm Bank 'Loredana Gandini' Department of Experimental Medicine, 'Sapienza' University of Rome, Italy.

Study Question: Is ageing associated with a decline in semen quality and molecular changes to human sperm?

Summary Answer: Semen quality declines with advancing age and characteristic molecular changes take place during the ageing process, including increased sperm DNA damage, altered sperm protamination and altered seminal plasma miRNA profile.

What Is Known Already: During ageing, the reproductive system is exposed to physiological changes and potentially damaging factors that may impair testicular function. Reactive oxygen species (ROS) can induce errors during DNA replication, transcription or post-transcriptional events (fragmentation, chromatin condensation abnormalities and protamine expression defects). Read More

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December 2018

Arterial and pulmonary hypertension: risk assessment and current pharmacological and interventional management.

Eur Heart J 2018 Dec;39(47):4127-4131

Imperial College and Consultant and Director of Research, Education & Development, Royal Brompton and Harefield Hospital Trust, London, UK.

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December 2018

MiR-23a-3p acts as an oncogene and potential prognostic biomarker by targeting PNRC2 in RCC.

Biomed Pharmacother 2018 Dec 11;110:656-666. Epub 2018 Dec 11.

Department of Urology, Peking University Shenzhen Hospital, Shenzhen, Guangdong 518036, P.R. China; Guangdong and Shenzhen Key Laboratory of Male Reproductive Medicine and Genetics, Institute of Urology, Peking University Shenzhen Hospital, Shenzhen PKU-HKUST Medical Center, Shenzhen, Guangdong 518036, P.R. China. Electronic address:

Background: Renal cell carcinoma (RCC) is a most common kidney malignancy, with atypical symptoms in the early stage and poor outcome in the late stage. Recently, emerging evidence revealed that some miRNAs play an essential role in the tumorigenesis and progression of RCC. Therefore, the aim of this study is that understand the detailed molecular mechanism of miR-23a-3p in RCC and identify its potential clinical value. Read More

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December 2018

Mass spectrometry imaging of low molecular weight metabolites in strawberry fruit (Fragaria x ananassa Duch.) cv. Primoris with Ag nanoparticle enhanced target.

Phytochemistry 2018 Dec 11;159:11-19. Epub 2018 Dec 11.

Rzeszów University of Technology, Faculty of Chemistry, 6 Powstańców Warszawy Ave., 35-959, Rzeszów, Poland.

Strawberry (Fragaria x ananassa Duch., Rosaceae) is the subject of many research studies due to its numerous features such as unique taste, aroma and health qualities. The distribution of low molecular weight metabolites belonging to aldehydes, ketones, alcohols, esters, organic acids, phenolics, amino acids and sugars classes within strawberry fruit cross-section was studied using mass spectrometry imaging (MSI) method with Ag nanoparticle enhanced target (AgNPET). Read More

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December 2018

Cyclodextrin-enhanced 1,4-dioxane treatment kinetics with TCE and 1,1,1-TCA using aqueous ozone.

Chemosphere 2018 Nov 29;219:335-344. Epub 2018 Nov 29.

Water Science and Management Program, New Mexico State University, MSC 3Q P.O. Box 30003, Las Cruces, NM 88003, USA; Plant & Environmental Science, New Mexico State University, MSC 3Q P.O. Box 30003, Las Cruces, NM 88003, USA. Electronic address:

Enhanced reactivity of aqueous ozone (O) with hydroxypropyl-β-cyclodextrin (HPβCD) and its impact on relative reactivity of O with contaminants were evaluated herein. Oxidation kinetics of 1,4-dioxane, trichloroethylene (TCE), and 1,1,1-trichloroethane (TCA) using O in single and multiple contaminant systems, with and without HPβCD, were quantified. 1,4-Dioxane decay rate constants for O in the presence of HPβCD increased compared to those without HPβCD. Read More

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November 2018

Advances and opportunities for the design of self-sufficient and spatially organized cell-free biocatalytic systems.

Curr Opin Chem Biol 2018 Dec 11;49:97-104. Epub 2018 Dec 11.

Heterogeneous biocatalysis laboratory, Instituto de Síntesis Química y Catálisis Homogénea (ISQCH-CSIC), University of Zaragoza, C/ Pedro Cerbuna 12, 50009, Zaragoza, Spain; ARAID, Aragon I+D foundation, Zaragoza, Spain. Electronic address:

During the past decades, biocatalysis has made important contributions to chemical manufacturing by using both whole-cell and cell-free biotransformation reactions. More recently, multi-enzyme systems that can run step-wise reactions in one-pot with high selectivity are increasingly being developed. The use of multiple isolated enzymes to perform a series of reactions offers operational and process advantages over the use of living or resting cells, but such cell free processes need to be optimized to meet industrial productivity and titer requirements. Read More

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December 2018

Computational conformational analysis of α-thrombin inhibitors possessing distinct scaffolds in aqueous solution and on Ala-sheet.

Authors:
Keigo Gohda

J Mol Graph Model 2018 Dec 3;87:165-171. Epub 2018 Dec 3.

Computer-aided Molecular Modeling Research Center, Kansai (CAMM-Kansai), 3-32-302, Tsuto-Otsuka, Nishinomiya, 663-8241, Japan. Electronic address:

Recent computational simulations on protein-ligand binding/unbinding have precisely been uncovering the ligand-binding process at the atomic level. In the process, the non-specific binding of ligands to the target site is suggested to occur before binding to the target. We in this study analyzed the conformations of ligands under the non-specific binding on a protein surface to figure out the differences in the conformational characteristics in aqueous solution using the 55-ns molecular dynamic simulation. Read More

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December 2018

Influence of drivers' psychological risk profiles on the effectiveness of traffic calming measures.

Accid Anal Prev 2018 Dec 11;123:243-255. Epub 2018 Dec 11.

Department of Surgical, Medical and Molecular Pathology and Critical Care Medicine, University of Pisa, Via Paradisa 2, 56126, Pisa, Italy.

Road traffic injuries represent a serious public health problem and are one of leading cause of death, injury and disability around the world. Road accidents are often caused by an accumulation of factors; however, drivers appear to be by far the most decisive one. The driver's behaviour is complex and depends on reflex (or involuntary) and voluntary driving actions. Read More

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December 2018

Identification of the full 26 cytochrome P450 (CYP) genes and analysis of their expression in response to benzo[α]pyrene in the marine rotifer Brachionus rotundiformis.

Comp Biochem Physiol Part D Genomics Proteomics 2018 Dec 4;29:185-192. Epub 2018 Dec 4.

Department of Biological Science, College of Science, Sungkyunkwan University, Suwon 16419, South Korea. Electronic address:

Cytochrome P450s (CYPs) are a large gene superfamily that are found in all living organisms. CYPs have a key role in detoxification of xenobiotics and endogenous chemicals. Although aquatic invertebrate CYPs and their detoxification mechanisms have been reported, little is known about interspecific comparison of CYPs and their detoxification mechanism in the rotifer Brachionus spp. Read More

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December 2018

Syngeneic, in contrast to allogeneic, mesenchymal stem cells have superior therapeutic potential following spinal cord injury.

J Neuroimmunol 2018 Nov 16;328:5-19. Epub 2018 Nov 16.

Department of Neurology and Neurosurgery, Karolinska University Hospital, BioClinicum, Solnavägen 30, Solna, Stockholm 17176, Sweden; Center for Molecular Medicine, Karolinska Institutet, Solna 17176, Stockholm, Sweden; Department of Clinical Neuroscience, Karolinska Institutet, Solna 17176, Stockholm, Sweden. Electronic address:

We evaluated the importance of histocompatibility of transplanted MSCs in terms of therapeutic potential. Mouse syngeneic MSCs or allogeneic MSCs were transplanted following SCI in mouse. In this study we found that syngeneic, but not allogeneic, MSCs alternatively activated macrophages resulting in a down-regulation of pro-inflammation. Read More

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November 2018

The protective effect of sophocarpine in osteoarthritis: An in vitro and in vivo study.

Int Immunopharmacol 2018 Dec 11;67:145-151. Epub 2018 Dec 11.

Department of Anesthesiology, The Second Affiliated Hospital of Wen Zhou Medical University, 109, Xueyuanxi Road, 325027 Wenzhou, China. Electronic address:

Background: Osteoarthritis (OA) is a type of degenerative joint disease affecting millions of individuals worldwide. However, there are currently no great inflammatory treatments available for it. Sophocarpine (SPC), one of the key bioactive compounds derived from Sophora flavescens, has shown remarkable anti-inflammatory effects. Read More

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December 2018

Effects of hypoxia-reoxygenation stress on mitochondrial proteome and bioenergetics of the hypoxia-tolerant marine bivalve Crassostrea gigas.

J Proteomics 2018 Dec 11. Epub 2018 Dec 11.

Department of Marine Biology, University of Rostock, Rostock, Germany; Department of Maritime Systems, Interdisciplinary Faculty, University of Rostock, Rostock, Germany.

Mitochondria are key intracellular targets of hypoxia-reoxygenation (H/R) stress due to their central role in generation of ATP and reactive oxygen species (ROS). Intertidal oysters Crassostrea gigas are adapted to frequent H/R cycles and maintain aerobic function despite frequent oxygen fluctuations. To gain insight into the molecular mechanisms of H/R tolerance, we assessed changes in mitochondrial respiration and (phospho)proteome of C. Read More

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December 2018

Early-life exposure to the endocrine disruptor 17-α-ethinylestradiol induces delayed effects in adult brain, liver and ovotestis proteomes of a self-fertilizing fish.

J Proteomics 2018 Dec 11. Epub 2018 Dec 11.

Laboratory of Evolutionary and Adaptive Physiology - Institute of Life, Earth and Environment - University of Namur, 61 Rue de Bruxelles, B5000 Namur, Belgium.

Early-life represents a critically sensitive window to endocrine disrupting chemicals, potentially leading to long-term repercussions on the phenotype later in life. The mechanisms underlying this phenomenon, referred to as the Developmental Origins of Health and Disease (DOHaD), are still poorly understood. To gain molecular understanding of these effects, we exposed mangrove rivulus (Kryptolebias marmoratus) for 28 days post hatching (dph) to 4 and 120 ng/L 17-α-ethinylestradiol, a model xenoestrogen. Read More

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December 2018

Rapid determination of 1,3-propanediol in fermentation process based on a novel surface-enhanced Raman scattering biosensor.

Spectrochim Acta A Mol Biomol Spectrosc 2018 Nov 28;211:227-233. Epub 2018 Nov 28.

Beijing Bioprocess Key Laboratory, Beijing University of Chemical Technology, Beijing 100029, PR China; College of Science, Beijing University of Chemical Technology, Beijing 100029, PR China. Electronic address:

The production of 1,3-propanediol (1,3-PDO) is an important fermentation process. However, 1,3-PDO could not be distinguished separately and efficiently in fermentations previously because it has a highly similar molecular structure to the feedstock glycerol (GLY) and by-product lactic acid (Lac), which leads to the difficulty of quantification. In this paper, a low-cost and environmentally friendly biosensor based on surface-enhanced Raman scattering (SERS) technique was developed. Read More

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November 2018

Identification of B cell epitopes enhanced by protein unfolding and aggregation.

Mol Immunol 2018 Dec 11;105:181-189. Epub 2018 Dec 11.

Lydia Becker Institute of Immunology and Inflammation, School of Biological Sciences, Faculty of Biology, Medicine and Health, Manchester Academic Health Science Centre, The University of Manchester, Oxford Road, Manchester, UK. Electronic address:

Aggregation of therapeutic proteins is a key factor in the generation of unwanted immunogenicity, and can result in reduced serum half-life, neutralization of function and adverse health effects. There is currently little information regarding how aggregates interact with B-cell receptors or cognate antibodies at the protein sequence level, or whether non-native, aggregate-induced epitopes predominate in these interactions. Using an antibody fragment (single chain antibody variable fragment; scFv) that forms aggregates readily at low temperature, anti-scFv IgG antibody responses were generated by intraperitoneal injection of BALB/c strain mice with monomer or aggregate preparations. Read More

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December 2018

Three monophyletic clusters in Retortamonas species isolated from vertebrates.

Parasitol Int 2018 Dec 11. Epub 2018 Dec 11.

Department of Parasitology, Graduate School of Medical Sciences, Kanazawa University, Kanazawa 920-8640, Japan. Electronic address:

Retortamonas spp. has been reported as an intestinal parasite among various host organisms, including humans; however, its intra-genus molecular diversity has not yet been elucidated. Haplotypes of the 18S small subunit ribosomal RNA locus (1836-1899 bp) of Retortamonas spp. Read More

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December 2018

A plastid phylogeny of the fern genus Arachniodes (Dryopteridaceae).

Mol Phylogenet Evol 2018 Dec 11. Epub 2018 Dec 11.

CAS Key Laboratory of Mountain Ecological Restoration and Bioresource Utilization, Chengdu Institute of Biology, Chinese Academy of Sciences, P.O. Box 416, Chengdu, Sichuan 610041, China; Missouri Botanical Garden,4344 Shaw Blvd., St. Louis, Missouri 63110, U.S.A. Electronic address:

Arachniodes (Dryopteridaceae) is one of the most confusing and controversial fern genera in terms of its circumscription, nomenclature, and taxonomy. Estimates of species number range from 40 to 200. Previous molecular works included only 2-17 accessions representing 2-12 species of Arachniodes and allied genera, leaving most of the Asian species remain unsampled and the infragneric relationships unclear. Read More

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December 2018

When is a "cryptic" species not a cryptic species: A consideration from the Holarctic micro-landsnail genus Euconulus (Gastropoda: Stylommatophora).

Mol Phylogenet Evol 2018 Dec 11. Epub 2018 Dec 11.

Department of Botany and Zoology, Masaryk University, Kotlářská 2, CZ-61137, Brno, Czechia. Electronic address:

Naive use of molecular data may lead to ambiguous conclusions, especially within the context of "cryptic" species. Here, we integrated molecular and morphometric data to evaluate phylogenetic relationships in the widespread terrestrial micro-snail genus, Euconulus. We analyzed mitochondrial (16S + COII) and nuclear (ITS1 + ITS2) sequence across 94 populations from Europe, Asia and North America within the nominate species E. Read More

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December 2018

Molecular systematics of the Triplophysa robusta (Cobitoidea) complex: extensive gene flow in a depauperate lineage.

Mol Phylogenet Evol 2018 Dec 11. Epub 2018 Dec 11.

Key Laboratory of Adaptation and Evolution of Plateau Biota, and Laboratory of Plateau Fish Evolutionary and Functional Genomics, and Qinghai Key Laboratory of Animal Ecological Genomics, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining 810008, Qinghai, China. Electronic address:

Gene flow between populations assumed to be isolated frequently leads to incorrect inferences of evolutionary history. Understanding gene flow and its causes has long been a key topic in evolutionary biology. In this study, we explored the evolutionary history of the Triplophysa robusta complex, using a combination of multilocus analyses and coalescent simulation. Read More

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December 2018

Integrating multilocus DNA data and 3D geometric morphometrics to elucidate species boundaries in the case of Pyrenaearia (Pulmonata: Hygromiidae).

Mol Phylogenet Evol 2018 Dec 11. Epub 2018 Dec 11.

Department of Zoology and Animal Cell Biology, Faculty of Pharmacy, University of the Basque Country, EHU/UPV, Paseo de la Universidad 7, 01006 Vitoria-Gasteiz, Araba, Spain; Biodiversity Research Group CIEA Lucio Lascaray, Avda. Miguel de Unamuno 3, 01006, Araba, Spain. Electronic address:

To accurately delimit species the use of multiple character types is essential as all speciation processes are not equally reflected in different data (e.g. morphological, molecular or ecological characters). Read More

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December 2018

Highly Efficient Production of Chiral Amines in Batch and Continuous Flow by Immobilized ω-Transaminases on Controlled Porosity Glass Metal-Ion Affinity Carrier.

J Biotechnol 2018 Dec 11. Epub 2018 Dec 11.

Van' t Hoff Institute for Molecular Sciences, HIMS-Biocat & HetCat, University of Amsterdam, Science Park 904, 1098 XH, The Netherlands. Electronic address:

In this study, two stereocomplementary ω-transaminases from Arthrobacter sp. (AsR-ωTA) and Chromobacterium violaceum (Cv-ωTA) were immobilized via iron cation affinity binding onto polymer-coated controlled porosity glass beads (EziG). The immobilization procedure was studied with different types of carrier materials and immobilization buffers of varying compositions, concentrations, pHs and cofactor (PLP) concentrations. Read More

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December 2018

CircRNAs and cancer: biomarkers and master regulators.

Semin Cancer Biol 2018 Dec 11. Epub 2018 Dec 11.

Molecular Oncology group, Biodonostia Research Institute, Paseo Doctor Begiristain, s/n San Sebastián, 20014, Spain; Radcliffe Department of Medicine, University of Oxford, John Radcliffe Hospital, Oxford, OX3 9DU, United Kingdom; IKERBASQUE, Basque Foundation for Science, María Díaz Haroko Kalea, 3, 48013, Bilbao, Spain. Electronic address:

Circular RNAs (circRNAs) are a novel class of regulatory RNAs that despite being relatively abundant have only recently begun to be explored. There are many thousands of genes that appear capable of producing circRNAs, however the function of all but a handful remain to be determined. What is emerging about these highly conserved molecules is that they play important roles in biology and cancer biology in particular. Read More

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December 2018

Targeting STATs in neuroinflammation: the Road Less Traveled!

Pharmacol Res 2018 Dec 11. Epub 2018 Dec 11.

Department of Pharmacology, Faculty of Pharmacy, Kermanshah University of Medical Sciences, Kermanshah, Iran.

JAK/STAT transduction pathway is a highly conserved pathway implicated in regulating cellular proliferation, differentiation, survival and apoptosis. Dysregulation of this pathway is involved in the onset of autoimmune, haematological, oncological, metabolic and neurological diseases. Over the last few years, the research of anti-neuroinflammatory agents has gained considerable attention. Read More

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December 2018

Characterization of locomotor response to psychostimulants in the parthenogenetic marbled crayfish (Procambarus fallax forma virginalis): A promising model for studying the neural and molecular mechanisms of drug addiction.

Behav Brain Res 2018 Dec 11. Epub 2018 Dec 11.

J.P. Scott Center for Neuroscience, Mind & Behavior, and Department of Biological Sciences, Bowling Green State University, Bowling Green, OH 43403, USA.

Although scientific research using mammalian models has made great strides in uncovering the enigmatic neural and molecular mechanisms orchestrating the state of drug addiction, a complete understanding has thus far eluded researchers. The complexity of the task has led to the use of invertebrate model systems to complement the research of drug-induced reward in mammalian systems. Invertebrates, such as crayfish, offer excellent model systems to help reveal the underlying mechanisms of drug addiction as they retain the ancestral neural reward circuit that is evolutionarily conserved across taxa, and they possess relatively few, large neurons, laid out in an accessible, modularly organized nervous system. Read More

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December 2018

Upregulation of the lactate transporter monocarboxylate transporter 1 at the blood-brain barrier in a rat model of attention-deficit/hyperactivity disorder suggests hyperactivity could be a form of self-treatment.

Behav Brain Res 2018 Dec 11. Epub 2018 Dec 11.

The Brain and Muscle Energy Group, Electron Microscopy Laboratory, Department of Oral Biology, University of Oslo, NO-0316 Oslo, Norway; Synaptic Neurochemistry and Amino Acid Transporters Labs, Division of Anatomy, Department of Molecular Medicine, Institute of Basic Medical Sciences (IMB) and Healthy Brain Ageing Centre (SERTA), University of Oslo, NO-0317 Oslo, Norway; Center for Healthy Aging, Department of Neuroscience and Pharmacology, Faculty of Health Sciences, University of Copenhagen, DK-2200 Copenhagen N, Denmark.

The energy deficit hypothesis of attention-deficit/hyperactivity disorder (ADHD) suggests that low lactate production by brain astrocytes causes the symptoms of the disorder. Astrocytes are the main producers of lactate in the brain; however, skeletal muscles can produce the most lactate in the body. The lactate production by skeletal muscles increases with physical activity, as does the expression of the lactate transporter monocarboxylate transporter 1 (MCT1) at the blood-brain barrier (BBB). Read More

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December 2018

Distinct regulation pattern of Egr-1, BDNF and Arc during morphine-withdrawal conditioned place aversion paradigm: role of glucocorticoids.

Behav Brain Res 2018 Dec 11. Epub 2018 Dec 11.

Department of Pharmacology, University of Murcia, Murcia, Spain; Murcia Institute of Biomedical Research (IMIB), University of Murcia, Spain.

Negative affective aspects of opiate abstinence contribute to the persistence of substance abuse. Importantly, interconnected brain areas involved in aversive motivational processes, such as the ventral tegmental area (VTA) and medial prefrontal cortex (mPFC), become activated when animals are confined to withdrawal-paired environments. In the present study, place aversion was elicited in sham and adrenalectomized (ADX) animals by conditioned naloxone-precipitated drug withdrawal following exposure to chronic morphine. Read More

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December 2018

Fluoxetine increases analgesic effects of morphine, prevents development of morphine tolerance and dependence through the modulation of L-type calcium channels expression in mice.

Behav Brain Res 2018 Dec 11. Epub 2018 Dec 11.

Department of Pharmacology, School of Pharmacy, Physiology Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran; Neuroanesthesia Laboratory, Department of Anesthesiology, Emory University School of Medicine, Atlanta, GA, USA. Electronic address:

Here, we aimed to investigate the effects of fluoxetine on morphine-induced analgesia, as well as preventive effects of it on morphine induced tolerance and dependence in mice. We also elucidate the involvement of L-type Ca channels in these phenomena. To induce morphine tolerance, mice were treated with morphine (50 mg/kg) for 3 consecutive days. Read More

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December 2018

Clinical and aetiological study of hand, foot and mouth disease in southern Vietnam, 2013-2015: inpatients and outpatients.

Int J Infect Dis 2018 Dec 11. Epub 2018 Dec 11.

Oxford University Clinical Research Unit, Ho Chi Minh City, Vietnam; Centre for Tropical Medicine and Global Health, Nuffield Department of Medicine, University of Oxford, Oxford, UK.

Background: Hand, foot and mouth disease (HFMD) has been associated with large outbreaks among young children, including severe illness and fatality, in the Asia-Pacific Region since 1997. Severe illness is often associated with enterovirus A71 (EV-A71). Vietnam experienced a large sustained outbreak of 200. Read More

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December 2018

Microfluidic assembly of hydrogel-based immunogenic tumor spheroids for evaluation of anticancer therapies and biomarker release.

J Control Release 2018 Dec 11. Epub 2018 Dec 11.

Department of Pharmaceutical Sciences, Northeastern University, 360 Huntington Avenue, Boston, MA 02115, USA. Electronic address:

Diffuse large B cell lymphoma (DLBCL), the most common subtype of Non-Hodgkin lymphoma, exhibits pathologic heterogeneity and a dynamic immunogenic tumor microenvironment (TME). However, the lack of preclinical in vitro models of DLBCL TME hinders optimal therapeutic screening. This study describes the development of an integrated droplet microfluidics-based platform for high-throughput generation of immunogenic DLBCL spheroids. Read More

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December 2018

Applications of π-π stacking interactions in the design of drug-delivery systems.

J Control Release 2018 Dec 11. Epub 2018 Dec 11.

College of Pharmacy and Research Institute of Pharmaceutical Sciences, Seoul National University, Seoul 08826, Republic of Korea. Electronic address:

Noncovalent forces are of considerable importance in the formation of self-assembled drug-delivery systems. In addition to non-destructively linking the delivery vehicle and guest drug, they provide multiple advantages, including protecting the structure of the drug, maintaining its functional effects, and facilitating its release. In particular, π-π stacking interactions have potential application in a comprehensive range of biomedical and biotechnological fields. Read More

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December 2018

Evaluation of three advanced methodologies, COLD-PCR, microarray and ddPCR, for identifying the mutational status by liquid biopsies in metastatic colorectal cancer patients.

Clin Chim Acta 2018 Dec 11. Epub 2018 Dec 11.

Unit of Genomic for the Diagnosis of Human Pathologies, Division of Genetics and Cell Biology, IRCCS San Raffaele Scientific Institute, Via Olgettina 60, Milan 20132, Italy; Laboratory of Clinical Molecular Biology, IRCCS Ospedale San Raffaele, Milan, Italy; Università Vita-Salute San Raffaele, Milano, Italy.

A major effort has been focused on the detection of oncogenes' mutations in diverse types of clinical specimens including formalin-fixed and paraffin embedded tissues, presently the gold-standard samples, up to plasma, that constitute a noninvasive alternative source of tumor DNA. The reliable detection of mutations in circulating tumor DNA requires a high analytical sensitivity. Here, we applied three different highly sensitive methodologies (COLD-PCR, a microarray-based approach and the droplet digital PCR, ddPCR) to identify mutations in the plasma of 30 metastatic colorectal cancer patients previously genotyped on tissue biopsy. Read More

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December 2018

Self-Assembled GFFYK Peptide Hydrogel Enhances the Therapeutic Efficacy of Mesenchymal Stem Cells in a Mouse Hindlimb Ischemia Model.

Acta Biomater 2018 Dec 11. Epub 2018 Dec 11.

Nankai University School of Medicine, Tianjin 300071, China; The Key Laboratory of Bioactive Materials, Ministry of Education, Nankai University, The College of Life Science, Tianjin 300071, China. Electronic address:

Mesenchymal stem cell (MSC) transplantation has emerged as a very promising strategy for the treatments of peripheral artery disease (PAD). However, MSC-based therapies are limited by low cell retention and survival rate in the ischemic zone. Small molecular (SM) hydrogels have shown attractive abilities to enhance the therapeutic effects of human MSCs via promoting their proliferation or maintaining their differentiation potential. Read More

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December 2018

Chronic Adolescent Stress Sex-Specifically Alters Central and Peripheral Neuro-Immune Reactivity in Rats.

Brain Behav Immun 2018 Dec 11. Epub 2018 Dec 11.

Virginia Commonwealth University, Department of Anatomy & Neurobiology, United States. Electronic address:

Adversity during development is a reliable predictor of psychiatric disorders such as depression and anxiety which are increasingly recognized to have an immune component. We have previously demonstrated that chronic adolescent stress (CAS) in rats leads to depressive-like behavior in adulthood along with long-lasting changes to the hypothalamic-pituitary-adrenal axis and pro-inflammatory cytokine induction in the hippocampus. However, the mechanisms by which CAS promotes hippocampal inflammation are not yet defined. Read More

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December 2018

Cyclical Treatment of Colorectal Tumor Spheroids Induces Resistance to MEK Inhibitors.

Transl Oncol 2018 Dec 11;12(3):404-416. Epub 2018 Dec 11.

Department of Biomedical Engineering, The University of Akron, Akron, OH 44325, USA. Electronic address:

Adaptive drug resistance is a major obstacle to successful treatment of colorectal cancers. Physiologic tumor models of drug resistance are crucial to understand mechanisms of treatment failure and improve therapy by developing new therapeutics and treatment strategies. Using our aqueous two-phase system microtechnology, we developed colorectal tumor spheroids and periodically treated them with sub-lethal concentrations of three Mitogen Activated Kinase inhibitors (MEKi) used in clinical trials. Read More

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December 2018

Uridine diphosphate release mechanism in O-N-acetylglucosamine (O-GlcNAc) transferase catalysis.

Biochim Biophys Acta Gen Subj 2018 Dec 11. Epub 2018 Dec 11.

The Key Laboratory of Natural Medicine and Immuno-Engineering, Henan University, Kaifeng 475004, People's Republic of China. Electronic address:

O-linked N-acetylglucosamine transferase (OGT) is an essential enzyme that catalyzes the covalent bonding of N-acetylglucosamine (GlcNAc) to the hydroxyl group of a serine or threonine in the target protein. It plays an important role in many important cellular physiological catalytic reactions. Here, we determine the binding mode and the binding free energy of the OGT product (uridine diphosphate, UDP) as well as the hydrogen-bond-dependent release mechanism using extensive molecular dynamic simulations. Read More

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December 2018

FTY720 inhibits the activation of pancreatic stellate cells by promoting apoptosis and suppressing autophagy via the AMPK/mTOR pathway.

Life Sci 2018 Dec 11. Epub 2018 Dec 11.

Department of Cell and Molecular Biology, Institute of Acute Abdominal Diseases of Integrated Traditional Chinese and Western Medicine, Tianjin Nankai Hospital, Tianjin 300100, China; Nankai Clinical College, Tianjin Medical University, Tianjin 300107, China. Electronic address:

Aims: Pancreatic stellate cells (PSCs) play a critical role in the development of pancreatic fibrosis. Any agents that can affect PSC activation could become potential candidates for treating pancreatic fibrosis. FTY720 can attenuate chronic pancreatic fibrosis by suppressing T-cell infiltration, but its effect on PSCs remains unknown. Read More

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December 2018

LncRNA PMS2L2 protects ATDC5 chondrocytes against lipopolysaccharide-induced inflammatory injury by sponging miR-203.

Life Sci 2018 Dec 11. Epub 2018 Dec 11.

Department of Orthopedics, Affiliated Hospital of Jining Medical University, Jining 272029, China. Electronic address:

Aims: PMS1 Homolog 2, Mismatch Repair System Component Pseudogene 2 (PMS2L2) has been reported as an up-regulated long non-coding RNA (lncRNA) in osteoarthritis (OA) tissues. The purpose of the present work is to explore whether the differently expressed PMS2L2 is associated with the pathogenesis of OA.

Main Methods: Chondrogenic ATDC5 cells were exposed to various doses of lipopolysaccharide (LPS). Read More

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December 2018

Proteomic analyses reveal lower expression of TEX40 and ATP6V0A2 proteins related to calcium ion entry and acrosomal acidification in asthenozoospermic males.

Life Sci 2018 Dec 11. Epub 2018 Dec 11.

Department of Biophysics, All India Institute of Medical Sciences, New Delhi 110029, India. Electronic address:

Aims: Idiopathic nature of male infertility disorder needs to be investigated by different horizons of molecular biology for its treatment and to device male contraceptive. Further, it can also aid in advancement of assisted reproductive technology (ART), as nowadays the failure and disquiets of ART are consistent. Herein, we have attempted to find out proteins responsible for male infertility by comparing proteome profile of sperms collected from normal control and asthenozoospermic (AS) males. Read More

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December 2018

Dissecting the Thermodynamic Contributions of the Charged Residues in the Membrane Anchoring of Bcl-xl C-Terminal Domain.

Chem Phys Lipids 2018 Dec 11. Epub 2018 Dec 11.

Bioinformatics Centre, Bose Institute, P-1/12 C.I.T. Scheme VII M, Kolkata, 700054, India. Electronic address:

The C-terminal helix of the Bcl-xl is known to initiate the membrane insertion of the protein by anchoring into the mitochondrial outer membrane. The C-terminal charged residues of that helix, R232, and K233, are reported to have an important structural role in the process of insertion. The present work provides a quantitative understanding of the thermodynamic contribution of these residues on the membrane insertion profile from Adaptive Biasing Force based MD simulations of 2. Read More

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December 2018

Expanding the Molecular Characterization of Thoracic Inflammatory Myofibroblastic Tumors beyond ALK gene rearrangements.

J Thorac Oncol 2018 Dec 11. Epub 2018 Dec 11.

Department of Pathology, Memorial Sloan Kettering Cancer Center, New York, NY. Electronic address:

Background: Half of inflammatory myofibroblastic tumors (IMTs) regardless of anatomic location harbor ALK gene rearrangements and overexpress ALK protein. The wide application of next generation sequencing (NGS) and the clinical benefit to tyrosine kinase inhibitors have opened new opportunities for investigation of ALK-negative IMT.

Design: In this study we investigate a series of pediatric and adult thoracic IMT for abnormalities in a wide spectrum of actionable kinases, by applying a variety of molecular and NGS techniques, including FISH, targeted RNA sequencing and NanoString. Read More

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December 2018

pSSbond-PseAAC: Prediction of disulfide bonding sites by integration of PseAAC and statistical moments.

J Theor Biol 2018 Dec 11. Epub 2018 Dec 11.

Gordon Life Science Institute, Boston, MA 02478, USA.

The structure of protein gains additional stability against various detrimental effects by the presence of disulfide bonds. The formation of correct disulfide bonds between cysteine residues ensures proper in vivo and in vitro folding of the protein. Many cysteine residues can be present in the polypeptide chain of a protein, however, not all cysteine residues are involved in the formation of a disulfide bond, and therefore, accurate prediction of these bonds is crucial for identifying biophysical characteristics of a protein. Read More

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December 2018
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