2,900,481 results match your criteria Molecular breeding : new strategies in plant improvement[Journal]


PTEN-deficient prostate cancer is associated with an immunosuppressive tumor microenvironment mediated by increased expression of IDO1 and infiltrating FoxP3+ T regulatory cells.

Prostate 2019 Apr 18. Epub 2019 Apr 18.

Cancer Biology and Genetics, Queen's Cancer Research Institute, Queen's University, Kingston, Canada.

Background: Accumulating evidence shows that tumor cell-specific genomic changes can influence the cross talk between cancer cells and the surrounding tumor microenvironment (TME). Loss of the PTEN tumor suppressor gene is observed in 20% to 30% of prostate cancers (PCa) when first detected and the rate increases with PCa progression and advanced disease. Recent findings implicate a role for PTEN in cellular type I interferon response and immunosuppression in PCa. Read More

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http://dx.doi.org/10.1002/pros.23808DOI Listing

[Acute Juvenile Ophthalmoplegia (Miller Fisher Syndrome): an Interesting Case].

Klin Monbl Augenheilkd 2019 Apr 18;236(4):469-471. Epub 2019 Apr 18.

Ophthalmica, Institute of Ophthalmology and Ocular Microsurgery, Thessaloniki, Griechenland.

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http://dx.doi.org/10.1055/a-0832-1815DOI Listing

Adipokines Regulate the Expression of Tumor-Relevant MicroRNAs.

Obes Facts 2019 Apr 18;12(2):211-225. Epub 2019 Apr 18.

Department of Anatomy and Cell Biology, Faculty of Medicine, Martin Luther University Halle-Wittenberg, Halle (Saale), Germany.

Background: Increasing prevalence of obesity requires the investigation of respective comorbidities, including tumor diseases like colorectal, renal, post-menopausal breast, prostate cancer, and leukemia. To date, molecular mechanisms of the malignant transformation of these peripheral tissues induced by obesity remain unclear. Adipose tissue secretes factors with hormone-like functions, the adipokines, and is therefore categorized as an endocrine organ. Read More

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http://dx.doi.org/10.1159/000496625DOI Listing

Interaction of BRAF-induced ETS factors with mutant TERT promoter in papillary thyroid cancer.

Endocr Relat Cancer 2019 Apr 1. Epub 2019 Apr 1.

Y Park, Internal medicine, Seoul National University College of Medicine, Seoul, Korea (the Republic of).

Synergistic effects of BRAFV600E and TERT promoter mutations on the poor clinical outcomes in papillary thyroid cancer (PTC) have been demonstrated. The potential mechanism of this phenomenon has been proposed: MAPK pathway activation by the BRAFV600E mutation may upregulate E-twenty six (ETS) transcription factors, increasing TERT expression by binding to the ETS-binding site generated by the TERT promoter mutation; however, it has not yet been fully proven. This article provides transcriptomic insights into the interaction between BRAFV600E and TERT promoter mutations mediated by ETS factors in PTC. Read More

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http://dx.doi.org/10.1530/ERC-17-0562DOI Listing

High soluble CGA levels are associated with poor survival in bladder cancer.

Endocr Connect 2019 Apr 1. Epub 2019 Apr 1.

H Reis, Institute of Pathology , University of Essen-Duisburg, Essen, Germany.

Recently, a neuroendocrine-like molecular subtype has been discovered in muscle-invasive urothelial bladder cancer (BC). Chromogranin A (CGA) is a widely used tissue and serum marker in neuroendocrine tumors. Our aim was to evaluate serum CGA (sCGA) concentrations and their associations with clinical and follow-up data in BC and renal cell carcinoma (RCC). Read More

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http://dx.doi.org/10.1530/EC-19-0068DOI Listing

Multiplatform metabolomics provides insight into the molecular basis of chronic kidney disease.

J Chromatogr B Analyt Technol Biomed Life Sci 2019 Apr 3;1117:49-57. Epub 2019 Apr 3.

Department of Biopharmaceutics and Pharmacodynamics, Medical University of Gdańsk, Gdańsk, Poland. Electronic address:

Changes in metabolites composition can reflect currently present pathological processes in a living organism and constitute a basis for diagnosis and treatment improvements. Thus, the multiplatform metabolomics approach was applied for the investigation of molecular mechanisms of chronic kidney disease (CKD) progression. The high-performance liquid chromatography coupled with time-of-flight mass spectrometry (HPLC-TOF-MS) and gas chromatography coupled with triple quadrupole mass spectrometry (GC-QqQ/MS) serum metabolic fingerprinting followed by uni- and multivariate statistical analysis was carried out to determine metabolic pattern differentiating CKD patients and healthy controls. Read More

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http://dx.doi.org/10.1016/j.jchromb.2019.04.003DOI Listing

Multiple distinct O-Mannosylation pathways in eukaryotes.

Curr Opin Struct Biol 2019 Apr 15;56:171-178. Epub 2019 Apr 15.

Copenhagen Center for Glycomics, Departments of Cellular and Molecular Medicine, Faculty of Health Sciences, University of Copenhagen, Blegdamsvej 3, DK-2200 Copenhagen N, Denmark. Electronic address:

Protein O-mannosylation (O-Man), originally discovered in yeast five decades ago, is an important post-translational modification (PTM) conserved from bacteria to humans, but not found in plants or nematodes. Until recently, the homologous family of ER-located protein O-mannosyl transferases (PMT1-7 in yeast; POMT1/POMT2 in humans), were the only known enzymes involved in directing O-Man biosynthesis in eukaryotes. However, recent studies demonstrate the existence of multiple distinct O-Man glycosylation pathways indicating that the genetic and biosynthetic regulation of O-Man in eukaryotes is more complex than previously envisioned. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S0959440X183017
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http://dx.doi.org/10.1016/j.sbi.2019.03.003DOI Listing
April 2019
1 Read

Unverricht-Lundborg disease: Clinical course and seizure management based on the experience of polish centers.

Authors:
Anetta Lasek-Bal

Seizure 2019 Apr 10;69:87-91. Epub 2019 Apr 10.

Department of Neurology, School of Health Sciences, Medical University of Silesia in Katowice, 40-635, Katowice, Poland. Electronic address:

The purpose of this paper was to present our experience following the longterm treatment of 11 patients with Unverricht-Lundborg disease (ULD) confirmed by molecular testing.

Methods: We analyzed the clinical course, cognitive state, neuroimaging and neurophysiology results.

Results: The data were collected from 9 unrelated families (F/M: 4/7) aged 25-49. Read More

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http://dx.doi.org/10.1016/j.seizure.2019.04.008DOI Listing

In silico analysis of different signal peptides for the secretory production of recombinant human keratinocyte growth factor in Escherichia coli.

Comput Biol Chem 2019 Mar 29;80:225-233. Epub 2019 Mar 29.

Department of Molecular Medicine, Biotechnology Research Center, Pasteur Institute of Iran, Tehran, Iran. Electronic address:

Background: The recombinant human truncated Keratinocyte growth factor (Palifermin) is the only FDA approved medicine for the treatment of oral mucositis. The Keratinocyte growth factor is a fairly unstable protein due to its high aggregation propensity and therefore its expression as a secretory protein may results in the production of a protein with more stability, higher solubility, better folding, enhanced biological activity, N-terminal authenticity and simplified downstream processing.

Objective: The aim of this study was in silico evaluation of 31 different secretory signal peptides to determine the best theoretical candidates for the secretory production of recombinant truncated human KGF in E. Read More

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http://dx.doi.org/10.1016/j.compbiolchem.2019.03.003DOI Listing

Potent and selective EGFR inhibitors based on 5-aryl-7H-pyrrolopyrimidin-4-amines.

Bioorg Chem 2019 Apr 11;88:102918. Epub 2019 Apr 11.

Department of Chemistry, Norwegian University of Science and Technology (NTNU), NO-7491 Trondheim, Norway. Electronic address:

The epidermal growth factor receptor represents an important target in cancer therapy, and low molecular weight inhibitors based on quinazolines have reached the marked. Herein we report on a new scaffold, 5-aryl-7H-pyrrolo[2,3-d]pyrimidin-4-amines, and show that when employing (S)-phenylglycinol as C-4 substituent, potent inhibitors can be made. The two most active inhibitors have suitable druglike properties, were equipotent with Erlotinib in Ba/F3 cell studies, and showed lower cross reactivity than Erlotinib in a panel of 50 kinases. Read More

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http://dx.doi.org/10.1016/j.bioorg.2019.102918DOI Listing

Vieloplains A-G, seven new guaiane-type sesquiterpenoid dimers from Xylopia vielana.

Bioorg Chem 2019 Apr 10;88:102891. Epub 2019 Apr 10.

Shanghai Key Laboratory for Molecular Engineering of Chiral Drugs, School of Pharmacy, Shanghai Jiao Tong University, Shanghai 200240, PR China; Institute of Interdisciplinary Sciences, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, PR China; School of Pharmacy, Second Military Medical University, Shanghai 200433, PR China. Electronic address:

Seven new guaiane-type sesquiterpene dimers vieloplains A-G, connecting patterns through three different direct CC bonds compounds 1-5 (C-3 to C-3', C-4 to C-1'), compound 6 (C-2 to C-3', C-4 to C-2') and compound 7 (C-2 to C-1', C-4 to C-2') were isolated from the roots of Xylopia vielana. Their absolute configurations were established by NOESY analysis, the Cu Kα X-ray crystallographic the experiment circular dichroism (ECD) and the calculated ECD. Among them, only compound 6 showed a considerable cytotoxicity against DU145 cells with IC values of 9. Read More

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http://dx.doi.org/10.1016/j.bioorg.2019.03.065DOI Listing

Computational approaches to macromolecular interactions in the cell.

Curr Opin Struct Biol 2019 Apr 15;55:59-65. Epub 2019 Apr 15.

Computational Biology Program and Department of Molecular Biosciences, The University of Kansas, Lawrence, KS 66045, USA. Electronic address:

Structural modeling of a cell is an evolving strategic direction in computational structural biology. It takes advantage of new powerful modeling techniques, deeper understanding of fundamental principles of molecular structure and assembly, and rapid growth of the amount of structural data generated by experimental techniques. Key modeling approaches to principal types of macromolecular assemblies in a cell already exist. Read More

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http://dx.doi.org/10.1016/j.sbi.2019.03.012DOI Listing

Gateway to the Golgi: molecular mechanisms of nucleotide sugar transporters.

Curr Opin Struct Biol 2019 Apr 15;57:127-134. Epub 2019 Apr 15.

Department of Biochemistry, University of Oxford, Oxford, OX1 3QU, UK. Electronic address:

The Golgi apparatus plays a central role in the secretory pathway as a hub for posttranslational modification, protein sorting and quality control. To date, there is little structural or biochemical information concerning the function of transporters that reside within this organelle. The SLC35 family of nucleotide sugar transporters link the synthesis of activated sugar molecules and sulfate in the cytoplasm, with the luminal transferases that catalyse their attachment to proteins and lipids during glycosylation and sulfation. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S0959440X183013
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http://dx.doi.org/10.1016/j.sbi.2019.03.019DOI Listing
April 2019
1 Read

More targeted use of oseltamivir and in-hospital isolation facilities after implementation of a multifaceted strategy including a rapid molecular diagnostic panel for respiratory viruses in immunocompromised adult patients.

J Clin Virol 2019 Apr 7;116:11-17. Epub 2019 Apr 7.

Department of Infectious Diseases, University Medical Center Utrecht, Utrecht University, Utrecht, the Netherlands.

Background: Immunocompromised adults are more vulnerable to a complicated course of viral respiratory tract infections (RTI).

Objectives: Provide evidence on the effect of implementation of rapid molecular diagnostics for viruses on use of in-hospital isolation facilities, oseltamivir and antibiotic usage, and other clinical outcomes in immunocompromised patients.

Study Design: A before-after study during two consecutive respiratory viral seasons, including immunocompromised adult patients presenting at a tertiary care emergency department with clinical suspicion of RTI. Read More

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http://dx.doi.org/10.1016/j.jcv.2019.04.003DOI Listing

Plant cell division - defining and finding the sweet spot for cell plate insertion.

Authors:
Sabine Müller

Curr Opin Cell Biol 2019 Apr 15;60:9-18. Epub 2019 Apr 15.

Center for Plant Molecular Biology, Auf der Morgenstelle 32, 72076 Tübingen, Germany. Electronic address:

The plant microtubules form unique arrays using acentrosomal microtubule nucleation pathways, yet utilizing evolutionary conserved centrosomal proteins. In cytokinesis, a multi-component cytoskeletal apparatus, the phragmoplast mediates the biosynthesis of the new cell plate by dynamic centrifugal expansion, a process that demands exquisite coordination of microtubule turnover and endomembrane trafficking. At the same time, the phragmoplast is guided to meet with the parental wall at a cortical site that is predefined before mitotic entry and transiently marked by the preprophase band of microtubules. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S09550674193001
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http://dx.doi.org/10.1016/j.ceb.2019.03.006DOI Listing
April 2019
1 Read

G-quadruplex and duplex DNA binding studies of novel Ruthenium(II) complexes containing ascididemin ligands.

J Inorg Biochem 2019 Apr 6;196:110681. Epub 2019 Apr 6.

Shanghai Key Laboratory of Chemical Assessment and Sustainability, School of Chemical Science and Engineering, Tongji University, Shanghai 200092, PR China. Electronic address:

In this paper, three new Ruthenium(II) polypyridyl complexes containing ascididemin (ASC) as main ligand have been synthesized and characterized. Their interactions with different G-quadruplex (Htelo, c-myc and c-kit) (Htelo: human telomeric DNA, c-myc: cellular-myelocytomatosis viral oncogene, c-kit: oncogene c-kit promoter sequences) and duplex (ds26) DNA sequences were comparatively studied with the free ligand ASC by a series of spectroscopic techniques including UV-vis (ultraviolet-visible) spectroscopy, FID (fluorescent intercalator displacement) assay, and FRET (fluorescence resonance energy transfer) melting assay. Molecular docking studies were also performed to support the binding mode of the compounds with G-quadruplex DNA. Read More

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http://dx.doi.org/10.1016/j.jinorgbio.2019.03.021DOI Listing

STIM1 structure-function and downstream signaling pathways.

Cell Calcium 2019 Apr 8;80:101-102. Epub 2019 Apr 8.

Institute of Biophysics, Johannes Kepler University Linz, Life Science Center, Gruberstrasse 40, 4020 Linz, Austria. Electronic address:

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http://dx.doi.org/10.1016/j.ceca.2019.01.004DOI Listing

Baicalein attenuates pancreatic inflammatory injury through regulating MAPK, STAT 3 and NF-κB activation.

Int Immunopharmacol 2019 Apr 15;72:204-210. Epub 2019 Apr 15.

Tianjin Key Laboratory of Chinese Medicine Pharmacology, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China; School of Integrative Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China. Electronic address:

Acute pancreatitis (AP) is a common acute abdominal disease with local or systemic inflammatory response, caused by abnormal activation of digestive enzymes. Baicalein has been shown to exert anti-inflammatory effects and to attenuate the pathological changes of AP. The aim of the research was to investigate the effects of baicalein on caerulein induced pancreatitis, and to elucidate the putative underlying mechanism. Read More

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http://dx.doi.org/10.1016/j.intimp.2019.04.018DOI Listing

Cluster binding studies with two anti-Thomsen-Friedenreich (anti-core-1, CD176, TF) antibodies: Evidence for a multiple TF epitope.

Int Immunopharmacol 2019 Apr 15;72:186-194. Epub 2019 Apr 15.

Glycotope GmbH, 13125 Berlin-Buch, Germany. Electronic address:

Antibodies to carbohydrate epitopes are often of the IgM isotype and require multiple binding for sufficient avidity. Therefore clusters of epitopes are preferred antigenic sites in these cases. We have examined the type of clusters recognized by two anti-Thomsen-Friedenreich (TF, core-1, CD176) IgM antibodies, NM-TF1 and NM-TF2, using several different sets of TF-carrying synthetic glycoconjugates in ELISA experiments. Read More

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http://dx.doi.org/10.1016/j.intimp.2019.03.058DOI Listing

Seasonal variations of NPAHs and OPAHs in PM at heavily polluted urban and suburban sites in North China: Concentrations, molecular compositions, cancer risk assessments and sources.

Ecotoxicol Environ Saf 2019 Apr 15;178:58-65. Epub 2019 Apr 15.

Environment Research Institute, Shandong University, Qingdao, Shandong 266237, China.

16 nitrated polycyclic aromatic hydrocarbons (NPAHs) and 5 oxygenated polycyclic aromatic hydrocarbons (OPAHs) in PM at two locations in Northern China were analyzed by Gas Chromatography-Mass Spectrometry (GC-MS). Sampling was conducted at an urban site in Shandong University in Jinan (SDU) and a suburban site in Qixingtai in Jinan (QXT) in March, June, September and December in 2016. Overall, the concentrations of NPAHs and OPAHs were higher at SDU (1. Read More

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http://dx.doi.org/10.1016/j.ecoenv.2019.04.009DOI Listing

Molecular cloning and mRNA expression of IFIT5 in tissues of ducklings infected with virulent duck hepatitis A virus type 3.

Res Vet Sci 2019 Apr 8;124:256-262. Epub 2019 Apr 8.

Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, Heilongjiang Province, PR China; Northeastern Science Inspection Station, China Ministry of Agriculture Key Laboratory of Animal Pathogen Biology, Northeast Agricultural University, Harbin 150030, PR China. Electronic address:

Interferon-induced proteins with tetratricopeptide repeats (IFITs) are a family of proteins strongly induced downstream of type I interferon signaling. The function of IFITs has been investigated extensively in mammals. IFIT5 is the sole protein in this family found in birds and little information is available about the function of avian IFIT5. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S00345288183132
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http://dx.doi.org/10.1016/j.rvsc.2019.04.003DOI Listing
April 2019
1 Read

Calu-3 cells are largely resistant to entry driven by filovirus glycoproteins and the entry defect can be rescued by directed expression of DC-SIGN or cathepsin L.

Virology 2019 Apr 3;532:22-29. Epub 2019 Apr 3.

Infection Biology Unit, German Primate Center - Leibniz Institute for Primate Research, Kellnerweg 4, 37077 Göttingen, Germany; Faculty of Biology and Psychology, Wilhelm-Weber-Str. 2, University Göttingen, 37073 Göttingen, Germany. Electronic address:

Priming of the viral glycoprotein (GP) by the cellular proteases cathepsin B and L (CatB, CatL) is believed to be essential for cell entry of filoviruses. However, pseudotyping systems that predominantly produce non-filamentous particles have frequently been used to prove this concept. Here, we report that GP-mediated entry of retroviral-, rhabdoviral and filoviral particles depends on CatB/CatL activity and that this effect is cell line-independent. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S00426822193009
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http://dx.doi.org/10.1016/j.virol.2019.03.020DOI Listing
April 2019
1 Read

Highly photostable two-photon NIR AIEgens with tunable organelle specificity and deep tissue penetration.

Biomaterials 2019 Apr 9;208:72-82. Epub 2019 Apr 9.

HKUST-Shenzhen Research Institute, No. 9 Yuexing 1st RD, South Area, Hi-tech Park, Nanshan, Shenzhen, 518057, China; Department of Chemistry, Hong Kong Branch of Chinese National Engineering Research Center for Tissue Restoration and Reconstruction, Institute for Advanced Study, Institute of Molecular Functional Materials, And Department of Chemical and Biological Engineering, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, 999077, China; Center for Aggregation-Induced Emission, SCUT-HKUST Joint Research Institute, State Key Laboratory of Luminescent Materials and Devices, South China University of Technology, Guangzhou, 510640, China. Electronic address:

Photostability is a particularly important parameter for fluorescence imaging especially long-term dynamic tracking in live samples. However, many organic fluorophores show poor photostability under one-photon and two-photon continuous irradiation. In addition, these traditional fluorophores also suffer from aggregation-caused quenching (ACQ) in aggregate state in insolvable water environment. Read More

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http://dx.doi.org/10.1016/j.biomaterials.2019.04.002DOI Listing

Evaluation of excitation schemes for indirect detection of N via solid-state HMQC NMR experiments.

J Magn Reson 2019 Apr 5;303:28-41. Epub 2019 Apr 5.

Univ. Lille, CNRS, Centrale Lille, ENSCL, Univ. Artois, UMR 8181 - UCCS - Unit of Catalysis and Chemistry of Solids, F-59000 Lille, France; Bruker Biospin, 34 rue de l'industrie, F-67166 Wissembourg, France. Electronic address:

It has previously been shown that N NMR spectra can be reliably obtained through indirect detection via HMQC experiments. This method exploits the transfer of coherence between single-(SQ) or double-quantum (DQ) N coherences, and SQ coherences of a suitable spin-1/2 'spy' nucleus, e.g. Read More

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http://dx.doi.org/10.1016/j.jmr.2019.04.004DOI Listing

Cochliobolus sp. acts as a biochemical modulator to alleviate salinity stress in okra plants.

Plant Physiol Biochem 2019 Apr 14;139:459-469. Epub 2019 Apr 14.

Department of Botany, Garden Campus, Abdul Wali Khan University, Mardan, Pakistan.

Salinity stress can severely affect the growth and production of the crop plants. Cheap and reliable actions are needed to enable the crop plants to grow normal under saline conditions. Modification at the molecular level to produce resistant cultivars is one of the promising, yet highly expensive techniques, whereas application of endophytes on the other hand are very cheap. Read More

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http://dx.doi.org/10.1016/j.plaphy.2019.04.019DOI Listing

Comparative transcriptome analysis provides insights into the distinct germination in sheepgrass (Leymus chinensis) during seed development.

Plant Physiol Biochem 2019 Apr 13;139:446-458. Epub 2019 Apr 13.

Key Laboratory of Plant Resources, Institute of Botany, The Chinese Academy of Sciences, Beijing, China. Electronic address:

Sheepgrass (Leymus chinensis ((Trin.) Tzvel)) is an important perennial forage grass that is widely distributed in the Eurasia steppe. The seed germination percentage show significant variation among the different germplasm in sheepgrass. Read More

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http://dx.doi.org/10.1016/j.plaphy.2019.04.007DOI Listing

Carbenoxolone ameliorates insulin sensitivity in obese mice induced by high fat diet via regulating the IκB-α/NF-κB pathway and NLRP3 inflammasome.

Biomed Pharmacother 2019 Apr 15;115:108868. Epub 2019 Apr 15.

Department of Geriatrics, the Third Affiliated Hospital of Soochow University, Changzhou, Jiangsu, China.

The characteristic feature of obesity and insulin resistance is chronic low-grade inflammation. Nod-Like Receptor Pyrin 3 (NLRP3) inflammasome plays a central role in obesity-induced insulin resistance. However, how does Carbenoxolone (CBX) play its role in ameliorating insulin resistance in peripheral tissues of obese mice induced by high-fat diet (HFD) remains unknown. Read More

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http://dx.doi.org/10.1016/j.biopha.2019.108868DOI Listing

UCA1 promotes cell viability, proliferation and migration potential through UCA1/miR-204/CCND2 pathway in primary cystitis glandularis cells.

Biomed Pharmacother 2019 Apr 15;114:108872. Epub 2019 Apr 15.

Reproductive Medicine Center, Xiangya Hospital, Central South University, 87 Xiangya Road, Changsha, Hunan, 410008, China. Electronic address:

Cystitis glandularis (CG) is an unusual proliferative disorder of the urinary bladder. Increasing evidences demonstrated that long non-coding RNAs (lncRNAs) play important roles in a variety of cellular progresses. However, there are rarely reports about the role and underlying molecular mechanism of lncRNAs in CG. Read More

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http://dx.doi.org/10.1016/j.biopha.2019.108872DOI Listing

Multicenter evaluation of a novel ROS1 immunohistochemistry assay (SP384) for detection of ROS1 rearrangements in a large cohort of lung adenocarcinoma patients.

J Thorac Oncol 2019 Apr 15. Epub 2019 Apr 15.

Université Côte d'Azur, University Hospital Federation OncoAge, Laboratory of Clinical and Experimental Pathology, Pasteur Hospital, Nice, France;; Université Côte d'Azur, CNRS, INSERM, Institute of Research on Cancer and Ageing of Nice (IRCAN), University Hospital Federation OncoAge, Nice, France;; Université Côte d'Azur, University Hospital Federation OncoAge, Hospital-Related Biobank (BB-0033-00025), Pasteur Hospital, Nice, France;. Electronic address:

Introduction: The detection of a ROS1 rearrangement in advanced and metastatic lung adenocarcinoma (LUAD) lead to a targeted treatment with tyrosine kinase inhibitors, with favorable progression-free survival and overall survival of the patients. Thus, it is mandatory to screen for the ROS1 rearrangement in all these patients. ROS1 rearrangements can be detected using break-apart fluorescence in situ hybridization (FISH), which is the "gold standard"; however, ROS1 immunohistochemistry (IHC) can be used as a screening test since it is widely available, easy and rapid to perform, and cost-effective. Read More

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http://dx.doi.org/10.1016/j.jtho.2019.03.024DOI Listing

Molecular characterization of manganese superoxide dismutase (MnSOD) from sterlet Acipenser ruthenus and its responses to Aeromonas hydrophila challenge and hypoxia stress.

Comp Biochem Physiol A Mol Integr Physiol 2019 Apr 15. Epub 2019 Apr 15.

National Freshwater Fisheries Engineering Technology Research Center, Ministry of Science and Technology of China, Beijing Key Laboratory of Fishery Biotechnology (No.BZ0301), Beijing Fisheries Research Institute, Beijing 100068, China. Electronic address:

A novel gene encoding the mitochondrial manganese superoxide dismutase from sterlet Acipenser ruthenus (Ar-MnSOD) was cloned. The full-length cDNA of MnSOD was of 1040 bp with a 672 bp open reading frame encoding 224 amino acids and the deduced amino acid sequence was located in mitochondria. Sequence comparison analysis showed that Ar-MnSOD was highly similar to MnSODs of invertebrates and vertebrates, especially those of freshwater Cyprinidae fishes and mammals. Read More

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http://dx.doi.org/10.1016/j.cbpa.2019.04.010DOI Listing

Molecular regulation of myoendothelial gap junctions.

Curr Opin Pharmacol 2019 Apr 15;45:16-22. Epub 2019 Apr 15.

Walter-Brendel-Centre of Experimental Medicine, University Hospital, LMU Munich, Biomedical Centre, Cardiovascular Physiology, LMU Munich and German Centre for Cardiovascular Research, Partner Site Munich Heart Alliance, Munich, Germany.

Myoendothelial gap junctions are involved in the regulation of vascular tone. The major connexins described in the vascular system are Cx37, Cx40, Cx43, and Cx45 with all but Cx45 found in myoendothelial connections. Although many reports on post-translational modifications of these connexins are available, only few groups have investigated their role in controlling myoendothelial communication and signal propagation. Read More

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http://dx.doi.org/10.1016/j.coph.2019.03.006DOI Listing

Role of DNA hypomethylation in lateral habenular nucleus in the development of depressive-like behavior in rats.

J Affect Disord 2019 Mar 22;252:373-381. Epub 2019 Mar 22.

Department of Physiology, College of Basic Medical Sciences, Jilin University, Changchun, Jilin 130021, China. Electronic address:

Background: Lateral habenula nucleus (LHb) has recently been noted for its role in stress-induced depressive disorder. Yet little is known about the mechanisms by which external stimuli or depression induces pathological alteration in the LHb.

Methods: Chronic unpredictable mild stress (CUMS) was employed to model depressive-like behaviors in adult rats. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S01650327183242
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http://dx.doi.org/10.1016/j.jad.2019.03.062DOI Listing
March 2019
1 Read

The Reference Genome Sequence of Scutellaria baicalensis Provides Insights into the Evolution of Wogonin Biosynthesis.

Mol Plant 2019 Apr 15. Epub 2019 Apr 15.

Shanghai Key Laboratory of Plant Functional Genomics and Resources, Shanghai Chenshan Botanical Garden, Shanghai Chenshan Plant Science Research Center, Chinese Academy of Sciences, Shanghai, China; John Innes Centre, Norwich, NR4 7UH UK. Electronic address:

Scutellaria baicalensis Georgi is important in Chinese Traditional Medicine where preparations of dried roots, 'Huang Qin', are used for liver and lung complaints including complementary cancer treatments. We report a high-quality reference genome sequence for S. baicalensis where 93% of the 408. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S16742052193013
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http://dx.doi.org/10.1016/j.molp.2019.04.002DOI Listing
April 2019
7 Reads

De novo Domestication: An Alternative Route toward New Crops for the Future.

Mol Plant 2019 Apr 15. Epub 2019 Apr 15.

National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan 430070, China. Electronic address:

Current global agricultural production must feed over 7 billion people. However, productivity varies greatly across the globe and is under threat from both increased competitions for land and climate change and associated environmental deterioration. Moreover, the increase in human population size and dietary changes are putting an ever-greater burden on agriculture. Read More

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http://dx.doi.org/10.1016/j.molp.2019.03.016DOI Listing

Altered dynamics may drift pathological fibrillization in membraneless organelles.

Biochim Biophys Acta Proteins Proteom 2019 Apr 15. Epub 2019 Apr 15.

MTA-DE Laboratory of Protein Dynamics, University of Debrecen, Hungary. Electronic address:

Protein phase transition can generate non-membrane bound cellular compartments, which can convert from liquid-like to solid-like states. While the molecular driving forces of phase separation have been largely understood, much less is known about the mechanisms of material-state conversion. We apply a recently developed algorithm to describe the weak interaction network of multivalent motifs, and simulate the effect of pathological mutations. Read More

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http://dx.doi.org/10.1016/j.bbapap.2019.04.005DOI Listing

The olfactory bulb proteotype differs across frontotemporal dementia spectrum.

J Proteomics 2019 Apr 15. Epub 2019 Apr 15.

Clinical Neuroproteomics Unit, Navarrabiomed, Complejo Hospitalario de Navarra (CHN), Universidad Pública de Navarra (UPNA), Irunlarrea, 3, 31008 Pamplona, Spain; Proteored-ISCIII, Proteomics Unit, Navarrabiomed, Complejo Hospitalario de Navarra (CHN), Universidad Pública de Navarra (UPNA), Irunlarrea 3, 31008 Pamplona, Spain; IdiSNA, Navarra Institute for Health Research, Pamplona, Spain, Irunlarrea 3, 31008 Pamplona, Spain. Electronic address:

Mild olfactory dysfunction has been observed in frontotemporal dementias (FTD). However, the underlying molecular mechanisms associated to this deficit are poorly understood. We applied quantitative proteomics to analyze pathological effects on the olfactory bulb (OB) from progressive supranuclear palsy (PSP) and frontotemporal lobar degeneration (FTLD-TDP43) subjects respect to elderly non-FTD group. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S18743919193011
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http://dx.doi.org/10.1016/j.jprot.2019.04.011DOI Listing
April 2019
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Adverse and hormetic effects in rats exposed for 12 months to low dose mixture of 13 chemicals: RLRS part III.

Toxicol Lett 2019 Apr 15. Epub 2019 Apr 15.

Center of Toxicology Science & Research, Medical School, University of Crete, Heraklion, Crete, Greece; Department of Analytical and Forensic Medical Toxicology, Sechenov University, Moscow 119991, Russian Federation. Electronic address:

The aim of the current study was to evaluate the effects of a mixture of thirteen common chemicals on rats, after a one-year exposure to doses around the acceptable daily intake (ADIs), using blood and urinary tests. The influence of low doses of the mixture on weight gain, water consumption, feed consumption and feed efficiency, biochemistry parameters, haematological parameters, blood lymphocytes subsets, serum inflammation profile and urine parameters was evaluated. Our mixture caused a moderate monotonic increase of the males' appetite and a non-monotonic increase of anabolism and a monotonic increase of appetite for the females. Read More

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http://dx.doi.org/10.1016/j.toxlet.2019.04.005DOI Listing

Reconciling the controversial data on the effects of C fullerene at the organismal and molecular levels using as a model Drosophila melanogaster.

Toxicol Lett 2019 Apr 15. Epub 2019 Apr 15.

Taras Shevchenko National University of Kyiv, Volodymyrska St., 64, 01601, Kyiv, Ukraine.

Fullerenes have attracted attention since their discovery as structural units of complex carbon nanostructures capable of transporting drugs and macromolecules. As such artificial nanomaterials are applied in biology and medicine, they are routinely scrutinized for their effects on living organisms. The results of such studies range from direct destabilizing effects on DNA molecules to amelioration of the toxic effects of known genotoxic agents. Read More

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http://dx.doi.org/10.1016/j.toxlet.2019.03.006DOI Listing

A brainstorm on the systematics of Turnera (Turneraceae, Malpighiales) caused by insights from molecular phylogenetics and morphological evolution.

Mol Phylogenet Evol 2019 Apr 15. Epub 2019 Apr 15.

Universidade Estadual de Feira de Santana, Departamento de Ciências Biológicas, Programa de Pós-graduação em Botânica, Av. Transnordestina s.n., Feira de Santana, 44036-900 Bahia, Brazil. Electronic address:

With 145 species, Turnera is the largest genus of Turneraceae (Malpighiales). Despite several morphotaxonomic and cytogenetic studies, our knowledge about the phylogenetic relationships in Turnera remains mainly based on morphological data. Here, we reconstruct the most comprehensive phylogeny of Turnera with molecular data to understand the morphological evolution within this group and to assess its circumscription and infrageneric classification. Read More

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http://dx.doi.org/10.1016/j.ympev.2019.04.015DOI Listing

Pathophysiological Role of TRPM2 in Age-Related Cognitive Impairment in Mice.

Neuroscience 2019 Apr 15. Epub 2019 Apr 15.

Department of Molecular Pharmacology, Graduate School of Pharmaceutical Sciences, Kyoto University, 46-29 Yoshida-Shimoadachi-cho, Sakyo-ku, Kyoto 606-8501, Japan.

Aging causes various functional changes, including cognitive impairment and inflammatory responses in the brain. Transient receptor potential melastatin 2 (TRPM2), a Ca-permeable channel expressed abundantly in immune cells, exacerbates inflammatory responses. Previously, we reported that TRPM2 on resident microglia plays a critical role in exacerbating inflammation, white matter injury, and cognitive impairment during chronic cerebral hypoperfusion; however, the physiological or pathophysiological role of TRPM2 during age-associated inflammatory responses remains unclear. Read More

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http://dx.doi.org/10.1016/j.neuroscience.2019.04.012DOI Listing

Genome-wide identification, phylogeny and function analysis of GRAS gene family in Dendrobium catenatum (Orchidaceae).

Gene 2019 Apr 15. Epub 2019 Apr 15.

Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences, Beijing 100193, China. Electronic address:

Background: In recent years, the molecular mechanism of plant growth and development has been reported in detail. GRAS genes, a plant-specific family of transcription factor, play critical roles in the process. GRAS transcription factors are associated with axillary shoot meristem formation, radial root patterning, phytohormones (gibberellins) signal transduction, light signaling, and abiotic or biotic stress. Read More

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http://dx.doi.org/10.1016/j.gene.2019.04.038DOI Listing

Anti-atherogenic effects of CD36-targeted epigallocatechin gallate-loaded nanoparticles.

J Control Release 2019 Apr 15. Epub 2019 Apr 15.

Department of Nutritional Sciences, Texas Tech University, Lubbock, TX 79409, USA. Electronic address:

Intimal macrophages play a critical role in atherosclerotic lesion initiation and progression by taking up oxidized low-density lipoprotein (oxLDL) and promoting inflammatory process. 1-(Palmitoyl)-2-(5-keto-6-octene-dioyl) phosphatidylcholine (KOdiA-PC), a major type of oxidized phosphatidylcholines (PC) found on oxLDL, has a high binding affinity to the macrophage scavenger receptor CD36 and participates in CD36-mediated recognition and uptake of oxLDL by intimal macrophages. We successfully synthesized epigallocatechin gallate (EGCG)-loaded nanoparticles (Enano), which were composed of EGCG, PC, (+) alpha-tocopherol acetate, and surfactant. Read More

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http://dx.doi.org/10.1016/j.jconrel.2019.04.018DOI Listing

The key role of the elongation factors in the origin of the organization of the genetic code.

Biosystems 2019 Apr 15. Epub 2019 Apr 15.

The Ionian School, Genetic Code and tRNA Origin Laboratory, Via Roma 19, 67030, Alfedena, L'Aquila, Italy; Institute of Biosciences and Bioresources, CNR, Via P. Castellino, 111, 80131, Naples, Italy. Electronic address:

I suggest a model based on primordial elongation factors able to explain a relevant aspect of the organization of the code itself: why the biosynthetic relationships between amino acids are mainly allocated on the rows of the genetic code. I hypothesize the existence of specific primordial elongation factors able to recognize two aspects of the ancestral aminoacylated-tRNA: the third base of the anticodon and a distinctive recognition sequence, i.e. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S03032647193009
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http://dx.doi.org/10.1016/j.biosystems.2019.04.009DOI Listing
April 2019
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Differential Expression of HMGA1 and HMGA2 in pituitary neuroendocrine tumors.

Mol Cell Endocrinol 2019 Apr 15. Epub 2019 Apr 15.

Laboratory of Experimental Endocrinology - LEEx, Institute of Biomedical Sciences, Federal University of Rio de Janeiro, Brazil; Graduate Program in Endocrinology, Faculty of Medicine, Federal University of Rio de Janeiro, Brazil; Graduate Program in Pharmacology and Medicinal Chemistry, Institute of Biomedical Sciences, Federal University of Rio de Janeiro, Brazil. Electronic address:

Defining biomarkers for invasive pituitary neuroendocrine tumors (PitNETs) is highly desirable. The high mobility group A (HMGA) proteins are among the most widely expressed cancer-associated proteins. Indeed, their overexpression is a frequent feature of human malignancies, including PitNETs. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S03037207193011
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http://dx.doi.org/10.1016/j.mce.2019.04.010DOI Listing
April 2019
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Lipoxygenase drives Lipidomic and metabolic reprogramming in ischemic heart failure.

Metabolism 2019 Apr 15. Epub 2019 Apr 15.

Division of Cardiovascular Disease, Department of Medicine, The University of Alabama at Birmingham, AL, United States.

Background: After myocardial infarction (MI), delayed progression or reversal of cardiac remodeling is a prime target to limit advanced chronic heart failure (HF). However, the temporal kinetics of lipidomic and systemic metabolic signaling is unclear in HF. There is no consensus on metabolic and lipidomic signatures that influence structure, function, and survival in HF. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S00260495193007
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http://dx.doi.org/10.1016/j.metabol.2019.04.011DOI Listing
April 2019
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Comparative transcriptome analysis of human conjunctiva between normal and conjunctivochalasis persons by RNA sequencing.

Exp Eye Res 2019 Apr 15. Epub 2019 Apr 15.

Department of Ophthalmology, Putuo Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai, 200062, China.

Conjunctivochalasis (CCH) is a common ocular disease, especially in aged people. However, the molecular mechanism of CCH on transcriptional level has been unclear. In this study, we characterized the transcriptional landscape of human conjunctiva and compared the transcriptome between normal persons (n = 10) and CCH patients (n = 11). Read More

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http://dx.doi.org/10.1016/j.exer.2019.04.005DOI Listing

Unusual clinical description of adult with Timothy syndrome, carrier of a new heterozygote mutation of CACNA1C.

Eur J Med Genet 2019 Apr 15. Epub 2019 Apr 15.

Service Génétique, Génétique Clinique, CHU, Caen, France; Normandy University, UNICAEN, BIOTARGEN, Caen, France.

CANAC1C encodes for the main cardiac L-type calcium channel and mutations on it lead to a prolonged QT interval in Timothy Syndrome (TS). We provide a new de novo constitutional heterozygote missense variation in CACNA1C in a living adult woman, also carrier of the known c.2146-1G>C heterozygous variation of PKP2 inherited from her father. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S17697212183083
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http://dx.doi.org/10.1016/j.ejmg.2019.04.005DOI Listing
April 2019
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