2,083 results match your criteria Molecular biotechnology[Journal]


Biochemical, Kinetic, and Computational Structural Characterization of Shikimate Kinase from Methicillin-Resistant Staphylococcus aureus.

Mol Biotechnol 2019 Feb 12. Epub 2019 Feb 12.

Facultad de Medicina y Nutrición, Universidad Juárez del Estado de Durango, Av. Universidad y Fanny Anitua S/N, C.P. 34000, Durango, Dgo, Mexico.

One of the most widespread pathogens worldwide is methicillin-resistant Staphylococcus aureus, a bacterium that provokes severe life-threatening illnesses both in hospitals and in the community. The principal challenge lies in the resistance of MRSA to current treatments, which encourages the study of different molecular targets that could be used to develop new drugs against this infectious agent. With this goal, a detailed characterization of shikimate kinase from this microorganism (SaSK) is described. Read More

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http://link.springer.com/10.1007/s12033-019-00159-5
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http://dx.doi.org/10.1007/s12033-019-00159-5DOI Listing
February 2019
2 Reads
1.876 Impact Factor

Correction to: Production of a Recombinant Dermaseptin Peptide in Nicotiana tabacum Hairy Roots with Enhanced Antimicrobial Activity.

Mol Biotechnol 2019 Feb 12. Epub 2019 Feb 12.

Department of Agricultural Biotechnology, Faculty of Agricultural Sciences, University of Guilan, Rasht, 4199613776, Iran.

The original version of this article unfortunately contained a mistake in the unit "μg/l". The unit "μg/l" should be corrected to "μg/ml" throughout the paper. Read More

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http://link.springer.com/10.1007/s12033-019-00157-7
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http://dx.doi.org/10.1007/s12033-019-00157-7DOI Listing
February 2019
1 Read

Responsiveness and Adaptation to Salt Stress of the REDOX-RESPONSIVE TRANSCRIPTION FACTOR 1 (RRTF1) Gene are Controlled by its Promoter.

Mol Biotechnol 2019 Feb 7. Epub 2019 Feb 7.

Center for Plant Sciences, University of Leeds, Leeds, LS2 9JT, UK.

The REDOX-RESPONSIVE TRANSCRIPTION FACTOR 1 (RRTF1) gene encodes a member of the ERF/AP2 transcription factor family involved in redox homeostasis. The RRTF1 gene shows tissue-specific responsiveness to various abiotic stress treatments including a response to salt stress in roots. An interesting feature of this response is an adaptation phase that follows its activation, when promoter levels revert to a base line level, even if salt stress is maintained. Read More

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http://dx.doi.org/10.1007/s12033-019-00155-9DOI Listing
February 2019
1 Read

Analysis of In Vivo Activity of the Bovine Cholesterol Hydroxylase/Lyase System Proteins Expressed in Escherichia coli.

Mol Biotechnol 2019 Feb 7. Epub 2019 Feb 7.

A.N. Belozersky Institute of Physico-Chemical Biology, M.V. Lomonosov Moscow State University, Leninskie Gory, 1/40, Moscow, 119234, Russia.

The cholesterol hydroxylase/lyase (CHL) system, located in the mitochondria of the mammalian adrenal cortex cells, consists of cytochrome P450scc (CYP11A1), adrenodoxin (Adx), and adrenodoxin reductase (AdR) and performs the first stage of the steroidogenesis: AdR and Adx enable the electron transfer between NADPH and cytochrome P450scc, and P450scc catalyzes the conversion of cholesterol into pregnenolone. CHL system was reconstructed in Escherichia coli using the polycistronic plasmid pTrc99A/CHL. In E. Read More

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http://dx.doi.org/10.1007/s12033-019-00158-6DOI Listing
February 2019

Phage Display Libraries: From Binders to Targeted Drug Delivery and Human Therapeutics.

Authors:
Mouldy Sioud

Mol Biotechnol 2019 Feb 7. Epub 2019 Feb 7.

Department of Cancer Immunology, Oslo University Hospital, The Norwegian Radium Hospital, Montebello, 0310, Oslo, Norway.

The application of repertoire selection technologies for the study and characterization of tumor heterogeneity is an area of great interest in the field of tumor biology and immunotherapy. Among the most promising approaches, phage display has been successfully used to select peptides and antibody fragments to a variety of different targets, including cancer cells, immune cells and cytokines. Peptides selected from phage display have been used to guide the delivery of lytic peptides, cytotoxic drugs, and nanoparticles to cancer cells with the aim to obtain more efficient and less toxic therapeutics. Read More

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http://dx.doi.org/10.1007/s12033-019-00156-8DOI Listing
February 2019
2 Reads

Construction and Characterization of a Medium Copy Number Expression Vector Carrying Auto-Inducible dps Promoter to Overproduce a Bacterial Superoxide Dismutase in Escherichia coli.

Mol Biotechnol 2019 Feb 5. Epub 2019 Feb 5.

Laboratory of Pharmaceutical Biotechnology, School of Pharmacy, Institut Teknologi Bandung, Bandung, 40132, Indonesia.

Medium copy number expression vector and auto-inducible promoter could be a solution for producing recombinant therapeutic proteins in industrial scale regarding plasmid stability, cost, and product quality. This work aimed to construct a medium copy number pBR322-based expression vector carrying auto-inducible promoter, determine its ability to express heterologous gene, and study its segregational stability. Three stationary-phase promoters of Escherichia coli genes (gadA, dps and sbmC) were used to produce a superoxide dismutase from Staphylococcus equorum (rMnSODSeq) coding region from pBR322Δtet (pBR322-mini). Read More

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http://dx.doi.org/10.1007/s12033-018-00151-5DOI Listing
February 2019

The Growth Differentiation Factor 11 is Involved in Skin Fibroblast Ageing and is Induced by a Preparation of Peptides and Sugars Derived from Plant Cell Cultures.

Mol Biotechnol 2019 Mar;61(3):209-220

Arterra Bioscience srl, via B. Brin 69, 80142, Napoli, Italy.

Ageing is a complex and progressive phenomenon, during which the accumulation of morphological and chemical changes seriously compromises the capacity of the cells to proliferate and fulfil their biological tasks. The increase in the average age of the world population, associated with a higher occurrence of age-related diseases, is prompting scientific research to look for new strategies and molecular targets that may help in alleviating age-related phenotypes. Growth factors, responsible for modulating several aging markers in many tissues and organs, represent valuable targets to fight age-associated dysfunctions. Read More

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http://link.springer.com/10.1007/s12033-019-00154-w
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http://dx.doi.org/10.1007/s12033-019-00154-wDOI Listing
March 2019
3 Reads

Validated Nuclear-Based Transgene Expression Regulated by the Fea1 Iron-Responsive Promoter in the Green Alga Chlamydomonas reinhardtii.

Mol Biotechnol 2019 Jan 17. Epub 2019 Jan 17.

Institute of Bioprocess Engineering, Friedrich-Alexander-Universität Erlangen-Nürnberg, Paul-Gordan-Strasse 3, 91052, Erlangen, Germany.

Microalgae are in the focus for the production of recombinant proteins in research and potential commercial application. Inducible promoters represent important tools that potentially allow the expression of recombinant proteins at higher rates. In general, they are used to separate the culture growth phase from the production phase by initiating product formation after high cell densities have been achieved. Read More

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http://dx.doi.org/10.1007/s12033-018-00148-0DOI Listing
January 2019

Building Bridges Between Structural and Network-Based Systems Biology.

Mol Biotechnol 2019 Mar;61(3):221-229

Institute of Chemical Engineering Sciences, Foundation for Research & Technology-Hellas (FORTH/ICE-HT), 26504, Patras, Greece.

The integration of structural and network-based systems biology is paramount for the improved understanding of how the proteins interact to modulate the behavior of complex biological systems. This review presents the current literature on the computational studies that combine these two scientific fields focusing on two main approaches: network-based analysis of the structure and dynamics of proteins and the in silico reconstruction of protein-protein interaction (PPI) networks or expansion of existed protein interactomes driven by structural annotations. Last, to enrich the current knowledge of the topological properties of the protein structure networks and evaluate the capacity of the public structural annotations of protein domains to predict novel PPIs, further computational analyses, missing so far from the literature, were performed. Read More

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http://dx.doi.org/10.1007/s12033-018-0146-8DOI Listing

Production of a Recombinant Dermaseptin Peptide in Nicotiana tabacum Hairy Roots with Enhanced Antimicrobial Activity.

Mol Biotechnol 2019 Jan 16. Epub 2019 Jan 16.

Department of Agricultural Biotechnology, Faculty of Agricultural Sciences, University of Guilan, Rasht, 4199613776, Iran.

Expression of strong antimicrobial peptides in plants is of great interest to combat a wide range of plant pathogens. To bring the Dermaseptin B1 (DrsB1) peptide to the intimate contact of the plant pathogens cell wall surface, the DrsB1 encoding sequence was fused to the C-terminal part of the two copies of the chitin-binding domain (CBD) of the Avr4 effector protein and used for Agrobacterium rhizogenes-mediated transformation. The expression of the recombinant protein in the tobacco hairy roots (HRs) was confirmed by molecular analysis. Read More

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http://link.springer.com/10.1007/s12033-019-00153-x
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http://dx.doi.org/10.1007/s12033-019-00153-xDOI Listing
January 2019
8 Reads

Isolation and Expression Analysis of Three Types of α-Carbonic Anhydrases from the Antarctic Alga Chlamydomonas sp. ICE-L under Different Light Stress Treatments.

Mol Biotechnol 2019 Mar;61(3):200-208

College of Chemical Engineering, Qingdao University of Science and Technology, Qingdao, 266042, China.

Carbonic anhydrases (CAs) are a class of zinc-containing metalloenzymes that can reversibly catalyse the hydration reaction of carbon dioxide. Antarctic algae are the most critical component of the Antarctic ecosystem; algae can enter the carbon cycle food chain by fixing carbon dioxide from the air. In this study, the complete open reading frames (ORFs) of CA1 (GenBank ID KY826431), CA2 (GenBank ID KY826432), and CA3 (GenBank ID KY826433), encoding CAs in the Antarctic ice microalga Chlamydomonas. Read More

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http://link.springer.com/10.1007/s12033-018-00152-4
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http://dx.doi.org/10.1007/s12033-018-00152-4DOI Listing
March 2019
2 Reads

CRISPR/Cas9-Mediated Multiply Targeted Mutagenesis in Orange and Purple Carrot Plants.

Mol Biotechnol 2019 Mar;61(3):191-199

State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Horticulture, Nanjing Agricultural University, Nanjing, 210095, China.

The clustered regularly interspaced short palindromic repeats (CRISPR)-associated protein 9 (Cas9) system has been successfully used for precise genome editing in many plant species, including in carrot cells, very recently. However, no stable gene-editing carrot plants were obtained with CRISPR/Cas9 system to date. In the present study, four sgRNA expression cassettes, individually driven by four different promoters and assembled in a single CRISPR/Cas9 vector, were transformed into carrots using Agrobacterium-mediated genetic transformation. Read More

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http://dx.doi.org/10.1007/s12033-018-00150-6DOI Listing

Understanding mA Function Through Uncovering the Diversity Roles of YTH Domain-Containing Proteins.

Mol Biotechnol 2019 Jan 12. Epub 2019 Jan 12.

College of Animal Sciences, Zhejiang University, No. 866 Yuhangtang Road, Hangzhou, 310058, Zhejiang, China.

N-methyladenosine (mA) is the most abundant-internal modification of eukaryotic mRNA. mA can be installed and removed by specific enzymes. The "writer," "eraser," and "reader" of mA modification have been reported. Read More

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http://dx.doi.org/10.1007/s12033-018-00149-zDOI Listing
January 2019

Development of Soybean Yellow Mottle Mosaic Virus-Based Expression Vector for Heterologous Protein Expression in French Bean.

Mol Biotechnol 2019 Mar;61(3):181-190

Advanced Centre for Plant Virology, Division of Plant Pathology, Indian Agricultural Research Institute, New Delhi, 110012, India.

Plant virus-based vectors provide attractive and valuable tools for rapid production of recombinant protein in large quantities as they produce systemic infections in differentiated plant tissues. In the present study, we engineered the Soybean yellow mottle mosaic virus (SYMMV) as a gene expression vector which is a promising candidate for systemic expression of foreign proteins in French bean plants. Full virus vector strategy was exploited for insertion of foreign gene by inserting MCS through PCR in the circular pJET-SYMMV clone. Read More

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http://dx.doi.org/10.1007/s12033-018-0147-7DOI Listing
March 2019
1 Read
1.876 Impact Factor

Ethylene Response Factor (ERF) Family Proteins in Abiotic Stresses and CRISPR-Cas9 Genome Editing of ERFs for Multiple Abiotic Stress Tolerance in Crop Plants: A Review.

Mol Biotechnol 2019 Feb;61(2):153-172

Biotechnology Group, Biological Sciences and Technology Division, CSIR-NEIST, Jorhat, Assam, 785006, India.

Abiotic stresses such as extreme heat, cold, drought, and salt have brought alteration in plant growth and development, threatening crop yield and quality leading to global food insecurity. Many factors plays crucial role in regulating various plant growth and developmental processes during abiotic stresses. Ethylene response factors (ERFs) are AP2/ERF superfamily proteins belonging to the largest family of transcription factors known to participate during multiple abiotic stress tolerance such as salt, drought, heat, and cold with well-conserved DNA-binding domain. Read More

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http://dx.doi.org/10.1007/s12033-018-0144-xDOI Listing
February 2019
1.876 Impact Factor

Improved Delivery of CRISPR/Cas9 System Using Magnetic Nanoparticles into Porcine Fibroblast.

Mol Biotechnol 2019 Mar;61(3):173-180

Department of Biochemistry and Biotechnology, Poznan University of Life Sciences, Dojazd 11, 60-632, Poznan, Poland.

Genetically modified pigs play an important role in agriculture and biomedical research; hence, new efficient methods are needed to obtain genetically engineered cells and animals. The clustered regularly interspaced short palindromic repeats (CRISPR)/Cas (CRISPR-associated) system represents an effective genome editing tool. It consists of two key molecules: single guide RNA (sgRNA) and the Cas9 endonuclease that can be introduced into the cells as one plasmid. Read More

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http://dx.doi.org/10.1007/s12033-018-0145-9DOI Listing
March 2019
4 Reads

Engineering Chlamydomonas reinhardtii for Expression of Functionally Active Human Interferon-α.

Mol Biotechnol 2019 Feb;61(2):134-144

Biology Department, Faculty of Science Yanbu, Taibah University, King Khalid Rd, Al amoedi, Yanbu El-Bahr, 46423, Saudi Arabia.

Human interferon (IFN) are secreted cytokines that play a major regulatory role in response to various infections. Commercially, IFN-α has been approved to treat many chronic viral diseases as well as a variety of cancers and different types of leukemia. In this study, a binary vector containing human IFN-α2a gene under the regulation of the cauliflower mosaic virus 35S promoter was constructed. Read More

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http://link.springer.com/10.1007/s12033-018-0143-y
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http://dx.doi.org/10.1007/s12033-018-0143-yDOI Listing
February 2019
3 Reads

New Nuclease from Extremely Psychrophilic Microorganism Psychromonas ingrahamii 37: Identification and Characterization.

Mol Biotechnol 2019 Feb;61(2):122-133

Department of Biomedical Chemistry, Faculty of Chemistry, University of Gdańsk, ul. Wita Stwosza 63, 80-308, Gdańsk, Poland.

Nucleases are an important group of hydrolases that degrade nucleic acids, with broad spectrum of applications in science and industry. In this paper, we report the identification and characterization of the nuclease from extremely psychrophilic bacterium Psychromonas ingrahamii that grows exponentially at 5 °C, but may also grow at even lower temperatures (down to - 12 °C). The putative endonuclease I gene, identified in the genome of P. Read More

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http://dx.doi.org/10.1007/s12033-018-0142-zDOI Listing
February 2019
1 Read

Generation and Characterization of Fibroblast-Specific Basigin Knockout Mice.

Mol Biotechnol 2019 Feb;61(2):111-121

Department of Cell Biology, National Translational Science Center for Molecular Medicine, State Key Laboratory of Cancer Biology, Fourth Military Medical University, No. 169 Changle West Road, Xi'an, 710032, China.

Basigin is a well-known extracellular stimulator of fibroblasts and may confer resistance to apoptosis of fibroblasts in vitro under some pathological status, but its exact function in fibroblasts and the underlying mechanism remain poorly understood. The systematic Basigin gene knockout leads to the perinatal lethality of mice, which limits the delineation of its function in vivo. In this study, we generated a fibroblast-specific Basigin knock-out mouse model and demonstrated the successful deletion of Basigin in fibroblasts. Read More

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http://dx.doi.org/10.1007/s12033-018-0141-0DOI Listing
February 2019
1 Read

Expression of Recombinant PfCelTOS Antigen in the Chloroplast of Chlamydomonas reinhardtii and its Potential Use in Detection of Malaria.

Mol Biotechnol 2019 Feb;61(2):102-110

Department of Biotechnology, Iranian Research Organization for Science and Technology, P.O. BOX: 3353-51111, Tehran, Iran.

Malaria is a serious but preventable and treatable infectious disease that is found in over 100 countries around the world. Correct and rapid diagnosis of malaria infection can rescue the patient of getting sicker and reduces the risk of disease spreading among humans. Chlamydomonas reinhardtii chloroplast is an attractive platform for expressing malaria antigens because it is capable of folding complex proteins, including those requiring disulfide bond formation, while lack the ability to glycosylate proteins; a valuable quality of any malaria protein expression system, since the Plasmodium parasite lacks N-linked glycosylation machinery. Read More

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http://link.springer.com/10.1007/s12033-018-0140-1
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http://dx.doi.org/10.1007/s12033-018-0140-1DOI Listing
February 2019
1 Read
1.876 Impact Factor

The Effect of Methyl Jasmonate and Temperature on the Transient Expression of Recombinant Proteins in Cucurbita pepo L.

Mol Biotechnol 2019 Feb;61(2):84-92

Department of Biotechnology, Faculty of Agriculture, Tarbiat Modares University, Tehran, Iran.

The aim of this study is to assess the effect of methyl jasmonate (MeJA) and temperature on the valuable pharmaceuticals expression in a virus-mediated transient expression system, and so the Zuchini Yellow Mosaic Virus (ZYMV) based vector was used for transferring the GFP reporter gene and recombinant tissue plasminogen activator (rtPA) gene (K2S) to cucurbit (Cucurbita pepo L.). MeJA, temperature and time (days after inoculation), were evaluated as a factorial experiment in a completely randomized design (CRD). Read More

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http://dx.doi.org/10.1007/s12033-018-0138-8DOI Listing
February 2019
1.876 Impact Factor

Strategies for the Construction of Cassava Brown Streak Disease Viral Infectious Clones.

Mol Biotechnol 2019 Feb;61(2):93-101

School of Biological Sciences, University of Bristol, 24 Tyndall Ave, Bristol, BS8 1TQ, UK.

Cassava brown streak disease (CBSD) has major impacts on yield and quality of the tuberous roots of cassava in Eastern and Central Arica. At least two Potyviridae species cause the disease: Cassava brown streak virus (CBSV) and Ugandan cassava brown streak virus (UCBSV). Cloned viral genome sequences known as infectious clones (ICs) have been important in the study of other viruses, both as a means of standardising infectious material and characterising viral gene function. Read More

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http://link.springer.com/10.1007/s12033-018-0139-7
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http://dx.doi.org/10.1007/s12033-018-0139-7DOI Listing
February 2019
11 Reads

Oxidative Damages to Eye Stem Cells, in Response to, Bright and Ultraviolet Light, Their Associated Mechanisms, and Salvage Pathways.

Mol Biotechnol 2019 Feb;61(2):145-152

Stem Cells and Regenerative Medicine Centre, Yenepoya Research Centre, Yenepoya (Deemed to be University), University Road, Derlakatte, Mangalore, Karnataka, 575018, India.

The surface of the eye is continuously exposed to the harshness of the external environment. As a homeostatic mechanism for replenishing the worn off cells of the ocular surface, a balance is maintained via the role of ocular stem cells. However, under extreme conditions of harshness like exposure to bright and ultraviolet light, the ocular stem cells are unable to do the repair mechanisms resulting in severe impairment of vision and disturbances in the eye. Read More

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http://link.springer.com/10.1007/s12033-018-0136-x
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http://dx.doi.org/10.1007/s12033-018-0136-xDOI Listing
February 2019
5 Reads
1.876 Impact Factor

DNA Vaccine Targeting Gonadotropin-Releasing Hormone Receptor and Its Application in Animal Contraception.

Mol Biotechnol 2019 Feb;61(2):73-83

Scott-Ritchey Research Center, College of Veterinary Medicine, Auburn University, 1265 HC Morgan Drive, Auburn, AL, 36849, USA.

Overpopulation of free-roaming and wildlife animals negatively affects economy and public health in many parts of the world. Contraceptive vaccines are viewed as a valuable option for reducing numbers of unwanted animals. This study develops vaccines for potential use in animal contraception exploiting a DNA platform. Read More

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http://link.springer.com/10.1007/s12033-018-0137-9
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http://dx.doi.org/10.1007/s12033-018-0137-9DOI Listing
February 2019
13 Reads

The Constitutive Expression of a Chrysanthemum ERF Transcription Factor Influences Flowering Time in Arabidopsis thaliana.

Mol Biotechnol 2019 Jan;61(1):20-31

Key Laboratory of Landscape Agriculture, College of Horticulture, Ministry of Agriculture, Nanjing Agricultural University, Weigang No. 1, Nanjing, 210095, Jiangsu, Republic of China.

AP2/ERF transcription factors (TFs) represent valuable targets for the genetic manipulation of crop plants, as they participate in the control of metabolism, growth and development, as well as in the plants' response to environmental stimuli. Here, an ERF TF encoded by the chrysanthemum (Chrysanthemum morifolium) genome, designated CmERF110, was cloned and functionally characterized. The predicted CmERF110 polypeptide included a conserved DNA-binding AP2/ERF domain. Read More

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http://dx.doi.org/10.1007/s12033-018-0134-zDOI Listing
January 2019

Effect of C-Terminus Modification in Salmonella typhimurium FliC on Protein Purification Efficacy and Bioactivity.

Mol Biotechnol 2019 Jan;61(1):12-19

Department of Immunology, Razi Vaccine and Serum Research Institute (RVSRI), Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran.

Recombinant flagellin (FliC) has shown low efficacy in purification because of inclusion bodies formation and aggregation. We hypothesized preserving TLR5 binding site of FliC and removing some amino acids could be responsible for aggregation and solubility improvement. Hence, a bioinformatics study was performed to find hotspots in aggregate formation. Read More

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http://dx.doi.org/10.1007/s12033-018-0135-yDOI Listing
January 2019
13 Reads

Ribosome Display: A Potent Display Technology used for Selecting and Evolving Specific Binders with Desired Properties.

Mol Biotechnol 2019 Jan;61(1):60-71

Department of Biochemical Engineering, School of Chemical Engineering & Technology, Tianjin University, Tianjin, 300350, People's Republic of China.

Ribosome display is a powerful engineering research tool for the high-throughput selection of peptides or proteins, which results in the generation of high-performance binders against nearly any antigen of interest. As a cell-free display system, ribosome display has been well developed with many outstanding achievements for over 20 years. Compared with other related display techniques, ribosome display shows unique advantages and development prospects. Read More

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http://dx.doi.org/10.1007/s12033-018-0133-0DOI Listing
January 2019

In-Silico Bioprospecting: Finding Better Enzymes.

Mol Biotechnol 2019 Jan;61(1):53-59

Department of Biological Sciences, Sunandan Divatia School of Science, NMIMS Deemed to be University, Vile Parle (W), Mumbai, 400056, India.

Enzymes are essential biological macromolecules, which catalyse chemical reactions and have impacted the human civilization tremendously. The importance of enzymes as biocatalyst was realized more than a century ago by eminent scientists like Kuhne, Buchner, Payen, Sumner, and the last three decades has seen exponential growth in enzyme industry, mainly due to the revolution in tools and techniques in molecular biology, biochemistry and production. This has resulted in high demand of enzymes in various applications like food, agriculture, chemicals, pharmaceuticals, cosmetics, environment and research sector. Read More

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http://link.springer.com/10.1007/s12033-018-0132-1
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http://dx.doi.org/10.1007/s12033-018-0132-1DOI Listing
January 2019
5 Reads

Correction to: Efficient Differentiation of Human Embryonic Stem Cells Toward Dopaminergic Neurons Using Recombinant LMX1A Factor.

Mol Biotechnol 2019 Jan;61(1):72

Department of Developmental Biology, University of Science and Culture, ACECR, Tehran, Iran.

The original version of this article was published without article note. The article note is given below. Read More

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http://dx.doi.org/10.1007/s12033-018-0131-2DOI Listing
January 2019
1 Read
1.880 Impact Factor

Ferulic Acid Produced by Lactobacillus fermentum Influences Developmental Growth Through a dTOR-Mediated Mechanism.

Mol Biotechnol 2019 Jan;61(1):1-11

Biomedical Technology and Cell Therapy Research Laboratory, Department of Biomedical Engineering, Faculty of Medicine, McGill University, 3775 University Street, Montreal, QC, H3A2B4, Canada.

The composition and activity of the gut microbiota impacts several energy-regulating conditions including diabetes, obesity and metabolic syndrome; however, the specific mechanisms linking the gut microbiota with the host's energy homeostasis remain elusive. Probiotics are health-promoting bacteria that when consumed, alter the composition and/or metabolism of resident microbiota conferring health benefits. To assess the role of a specific probiotic treatment on microbiota-derived impacts on energy homeostasis in the context of development, Drosophila melanogaster larvae were orally administered the probiotic Lactobacillus fermentum NCIMB 5221 or its metabolic product, ferulic acid: a potent anti-inflammatory and anti-oxidant hydroxycinnamic acid. Read More

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http://dx.doi.org/10.1007/s12033-018-0119-yDOI Listing
January 2019

The Research Progress of Chalcone Isomerase (CHI) in Plants.

Mol Biotechnol 2019 Jan;61(1):32-52

School of Life Sciences, Beijing University of Chinese Medicine, Yangguang South Street, Fangshan District, Beijing, 102401, China.

Chalcone isomerase (CHI) is the second rate-limiting and the first reported enzyme involved in the biosynthetic pathway of flavonoids. It catalyzes the intramolecular cyclization reaction, converting the bicyclic chalcone into tricyclic (2S)-flavanone. In this paper, we obtained and analyzed 916 DNA sequences, 1310 mRNA sequences, and 2403 amino acid sequences of CHI registered in NCBI by Jan 2018. Read More

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http://link.springer.com/10.1007/s12033-018-0130-3
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http://dx.doi.org/10.1007/s12033-018-0130-3DOI Listing
January 2019
9 Reads

Characterization and Evaluation of Mango Germplasm Through Morphological, Biochemical, and Molecular Markers Focusing on Fruit Production: An Overview.

Mol Biotechnol 2018 Oct 12. Epub 2018 Oct 12.

Genomics Lab, Department of Plant Breeding & Genetics, Bahauddin Zakariya University, Multan, 60800, Pakistan.

Mango is also known as 'King of Fruits' and is one of the most popular fruit crops in tropical and sub-tropical regions. Characterization through different markers is an important need for mango germplasm conservation and its utilization for various breeding purposes. Description of existing germplasm is essential to detect anticipated traits or genes. Read More

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http://dx.doi.org/10.1007/s12033-018-0129-9DOI Listing
October 2018

Avian Bioreactor Systems: A Review.

Mol Biotechnol 2018 Dec;60(12):975-983

Department of Animal Sciences, The Ohio State University, Columbus, OH, 43210, USA.

Animal bioreactors are genetically modified animal systems that have the potential to reduce production cost, and improve production efficiency, of pharmaceutically relevant recombinant proteins. Several species including goats, cattle, rabbits, and avians have been genetically modified to secrete target proteins into milk, egg whites, blood, or other bodily fluids. There are several advantages associated with the use of avians as bioreactor systems. Read More

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http://dx.doi.org/10.1007/s12033-018-0128-xDOI Listing
December 2018
1 Read

Expression, Purification, and Characterization of Recombinant Human α-Antitrypsin Produced Using Silkworm-Baculovirus Expression System.

Mol Biotechnol 2018 Dec;60(12):924-934

Laboratory of Insect Genome Science, Kyushu University Graduate School of Bioresource and Bioenvironmental Science, Hakozaki 6-10-1, Higashi-ku, Fukuoka, 812-8581, Japan.

Human α-antitrypsin (AAT) is the most abundant serine proteinase inhibitor (serpin) in the human plasma. Commercially available AAT for the medications of deficiency of α-antitrypsin is mainly purified from human plasma. There is a high demand for a stable and low-cost supply of recombinant AAT (rAAT). Read More

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http://link.springer.com/10.1007/s12033-018-0127-y
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http://dx.doi.org/10.1007/s12033-018-0127-yDOI Listing
December 2018
5 Reads

Identification and Mutational Analysis of Escherichia coli Sorbitol-Enhanced Glucose-Repressed srlA Promoter Expressed in LB Medium by Using Homologous Recombination and One-Round PCR Products.

Mol Biotechnol 2018 Dec;60(12):912-923

Department of Applied Chemistry, Graduate School of Sciences and Technology for Innovation, Yamaguchi University, Ube, 755-8611, Japan.

Escherichia coli has been used for recombinant protein production for many years. However, no native E. coli promoters have been found for constitutive expression in LB medium. Read More

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http://link.springer.com/10.1007/s12033-018-0123-2
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http://dx.doi.org/10.1007/s12033-018-0123-2DOI Listing
December 2018
11 Reads

Purification of Chinese Sacbrood Virus (CSBV), Gene Cloning and Prokaryotic Expression of its Structural Protein VP1.

Mol Biotechnol 2018 Dec;60(12):901-911

Key Laboratory of Pollinating Insect Biology, Ministry of Agriculture, Institute of Apicultural Research, Chinese Academy of Agricultural Sciences, Beijing, 100093, People's Republic of China.

The aim of this study was to purify the Chinese Sacbrood Virus Beijing Miyun (BJMY-CSBV) from infected Apis cerana larvae, clone structural protein gene VP1 (named BJMY-CSBV-VP1), and investigate its biological information. The result indicated that the capsid of CSBV is of spherical shape. Gene clone experiment showed that the BJMY-CSBV-VP1 gene sequence comprised 945 bp, encoding 315 amino acids with relative molecular weight of 35. Read More

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http://dx.doi.org/10.1007/s12033-018-0121-4DOI Listing
December 2018
1 Read

Tailoring Proteins to Re-Evolve Nature: A Short Review.

Mol Biotechnol 2018 Dec;60(12):946-974

Center for Research in Medical Sciences (CICMED), Autonomous University of the State of Mexico, 205 Venustiano Carranza, Universidad C.P. 50130, Toluca, Mexico.

Proteins are key biomolecules for most biological processes, their function is related to their conformation that is also dictated by their sequence of amino acids. Through evolution, nature has produced an immense variety of enzymatic tools of high efficiency and selectivity, and thanks to the understanding of the molecular basis of life and the technological advances, scientists have learned to introduce mutations and select mutant enzymes, to optimize and control their molecular fitness characteristics mainly for industrial, medical and environmental applications. The relationship between protein structure and enzymatic functionality is essential, and there are various experimental and instrumental techniques for unravelling the molecular changes, activities and specificities. Read More

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http://link.springer.com/10.1007/s12033-018-0122-3
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http://dx.doi.org/10.1007/s12033-018-0122-3DOI Listing
December 2018
20 Reads

Systematic Comparison of Strategies to Achieve Soluble Expression of Plasmodium falciparum Recombinant Proteins in E. coli.

Mol Biotechnol 2018 Dec;60(12):887-900

Laboratorio de Parasitología Molecular, Instituto de Biología Molecular, Universidad El Bosque, Edificio O. Segundo piso, Avenida Cra. 9 No. 131 A - 02, Bogotá, Colombia.

Constructs containing partial coding sequences of myosin A, myosin B, and glideosome-associated protein (50 kDa) of Plasmodium falciparum were used to challenge several strategies designed in order to improve the production and solubility of recombinant proteins in Escherichia coli. Assays were carried out inducing expression in a late log phase culture, optimizing the inductor concentration, reducing the growth temperature for induced cultures, and supplementing additives in the lysis buffer. In addition, recombinant proteins were expressed as fusion proteins with three different tags (6His, GST, and MBP) in four different E. Read More

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http://dx.doi.org/10.1007/s12033-018-0125-0DOI Listing
December 2018
1 Read

Molecular and Functional Diversity of RNA Editing in Plant Mitochondria.

Mol Biotechnol 2018 Dec;60(12):935-945

Department of Microbiology, University of North Carolina at Chapel Hill, Chapel Hill, NC, 27599, USA.

RNA editing is a fundamental biochemical process relating to the modification of nucleotides in messenger RNAs of functional genes in cells. RNA editing leads to re-establishment of conserved amino acid residues for functional proteins in nuclei, chloroplasts, and mitochondria. Identification of RNA editing factors that contributes to target site recognition increases our understanding of RNA editing mechanisms. Read More

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http://dx.doi.org/10.1007/s12033-018-0126-zDOI Listing
December 2018
2 Reads

Factors Affecting the Tailing of Blunt End DNA with Fluorescent Pyrimidine dNTPs.

Mol Biotechnol 2018 Dec;60(12):879-886

Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Vavilov Street 32, Moscow, Russia, 119991.

The transferase activity of non-proofreading DNA polymerases is a well-known phenomenon that has been utilized in cloning and sequencing applications. The non-templated addition of modified nucleotides at DNA blunt ends is a potentially useful feature of DNA polymerases that can be used for selective transformation of DNA 3' ends. In this paper, we characterized the tailing reaction at perfectly matched and mismatched duplex ends with Cy3- and Cy5-modified pyrimidine nucleotides. Read More

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http://dx.doi.org/10.1007/s12033-018-0124-1DOI Listing
December 2018
1 Read

Gene Delivery to Tobacco Root Cells with Single-Walled Carbon Nanotubes and Cell-Penetrating Fusogenic Peptides.

Mol Biotechnol 2018 Dec;60(12):863-878

Department of Biochemistry, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran.

Development of efficient, easy, and safe gene delivery methods is of great interest in the field of plant biotechnology. Considering the limitations of the usual transfection methods (such as transgene size and plant type), several new techniques have been tested for replacement. The success of some biological and synthetic nanostructures such as cell-penetrating peptides and carbon nanotubes in transferring macromolecules (proteins and nucleic acids) into mammalian cells provoked us to assess the ability of an engineered chimeric peptide and also arginine functionalized single-walled carbon nanotube in gene delivery to intact tobacco (Nicotiana tabacum var. Read More

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http://dx.doi.org/10.1007/s12033-018-0120-5DOI Listing
December 2018

Towards Three-Dimensional Dynamic Regulation and In Situ Characterization of Single Stem Cell Phenotype Using Microfluidics.

Mol Biotechnol 2018 Nov;60(11):843-861

Laboratory of Bioengineering, Earth and Life Institute, Université Catholique de Louvain, Place Croix du Sud 2, boîte L7.05.19, 1348, Louvain-la-Neuve, Belgium.

Mesenchymal stem cells and pluripotent stem cells are recognized as promising tools for tissue engineering, cell therapy, and drug screening. Their use in therapy requires the production of a sufficient number of cells committed to functional regenerative phenotypes. Time- and magnitude-controlled application of mechanical and biochemical cues is required to appropriately control the evolution of stem cell phenotype in 3D. Read More

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http://dx.doi.org/10.1007/s12033-018-0113-4DOI Listing
November 2018
15 Reads

A Novel Molecular Design for a Hybrid Phage-DNA Construct Against DKK1.

Mol Biotechnol 2018 Nov;60(11):833-842

Department of Medical Biotechnology, Faculty of Medical Sciences, School of Medical Sciences, Tarbiat Modares University, Tehran, Iran.

Nucleic acid immunization has recently exhibited a great promise for immunotherapy of various diseases. However, it is now clear that powerful strategies are imminently needed to improve their efficiency. In this regard, whole bacteriophage particles have been described as efficient DNA vaccine delivery vehicles, capable of circumventing the limitations of naked DNA immunization. Read More

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http://dx.doi.org/10.1007/s12033-018-0115-2DOI Listing
November 2018
2 Reads

Modulating the Expression Strength of the Baculovirus/Insect Cell Expression System: A Toolbox Applied to the Human Tumor Suppressor SMARCB1/SNF5.

Mol Biotechnol 2018 Nov;60(11):820-832

Stereology and Electron Microscopy Laboratory, Aarhus University, Wilhelm Meyers Allé 3, Building 1233, 8000, Aarhus C, Denmark.

The human tumor suppressor SMARCB1/INI1/SNF5/BAF47 (SNF5) is a core subunit of the multi-subunit ATP-dependent chromatin remodeling complex SWI/SNF, also known as Brahma/Brahma-related gene 1 (BRM/BRG1)-associated factor (BAF). Experimental studies of SWI/SNF are currently considerably limited by the low cellular abundance of this complex; thus, recombinant protein production represents a key to obtain the SWI/SNF proteins for molecular and structural studies. While the expression of mammalian proteins in bacteria is often difficult, the baculovirus/insect cell expression system can overcome limitations of prokaryotic expression systems and facilitate the co-expression of multiple proteins. Read More

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http://link.springer.com/10.1007/s12033-018-0107-2
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http://dx.doi.org/10.1007/s12033-018-0107-2DOI Listing
November 2018
13 Reads

IL-1 Fragment Modulates Immune Response Elicited by Recombinant Bacillus subtilis Spores Presenting an Antigen/Adjuvant Chimeric Protein.

Mol Biotechnol 2018 Nov;60(11):810-819

Department of Medical Biotechnology, Intercollegiate Faculty of Biotechnology UG-MUG, Medical University of Gdańsk, Gdańsk, Poland.

Mucosal immunizations are convenient ways of vaccination, which do not require any trained personnel for administration. One of the major challenges for developing an effective mucosal vaccine is finding appropriate adjuvant. Bacillus subtilis endospores have been shown to help solving these obstacles while serving as a platform for presentation of both, antigens and adjuvants. Read More

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http://dx.doi.org/10.1007/s12033-018-0117-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6182635PMC
November 2018
1 Read

Mutator-Based Transposon Display: A Genetic Tool for Evolutionary and Crop-Improvement Studies in Maize.

Mol Biotechnol 2018 Nov;60(11):799-809

Department of Applied Plant Sciences, College of Agriculture and Life Sciences, Kangwon National University, Chuncheon, 24341, South Korea.

Transposable elements account for up to 85% of the maize genome and have significant implications in crop-improvement and evolutionary analyses. The Mutator (Mu) transposon superfamily, a class of DNA transposons, comprises the most complex and active elements in the maize genome, suggesting a special role in plant evolution. Here, we designed a set of Mu-specific primers based on terminal invert repeats and used a transposon display (TD) method for genotyping. Read More

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http://link.springer.com/10.1007/s12033-018-0118-z
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http://dx.doi.org/10.1007/s12033-018-0118-zDOI Listing
November 2018
7 Reads

Identification of Potential Molecular Determinants of Murine Embryonic Stem Cell Differentiation by a Transposon-Based Approach.

Mol Biotechnol 2018 Nov;60(11):791-798

Department of Pediatrics, West China Second University Hospital, Sichuan University, Chengdu, 610041, Sichuan, China.

Embryonic stem cells (ESCs) are self-renewing pluripotent cells, capable of differentiating into all somatic cell types. The molecular control of self-renewal is relatively well-characterized, whereas how ESCs exit pluripotent state to differentiate is poorly understood. Here we identify two genes are required for differentiation and dozens of intergenic regions that potentially regulate ESC differentiation. Read More

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http://dx.doi.org/10.1007/s12033-018-0110-7DOI Listing
November 2018
1 Read

Purification and Biochemical Characterization of Phytase Enzyme from Lactobacillus coryniformis (MH121153).

Mol Biotechnol 2018 Nov;60(11):783-790

Department of Biochemistry, Faculty of Pharmacy, Anadolu University, 26470, Eskişehir, Turkey.

Phytase (myo-inositol hexaphosphate phosphohydrolase) belongs to phosphatases. It catalyzes the hydrolysis of phytate to less-phosphorylated inorganic phosphates and phytate. Phytase is used primarily for the feeding of simple hermit animals in order to increase the usability of amino acids, minerals, phosphorus and energy. Read More

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http://dx.doi.org/10.1007/s12033-018-0116-1DOI Listing
November 2018

DNA Vectors Generating Engineered Exosomes Potential CTL Vaccine Candidates Against AIDS, Hepatitis B, and Tumors.

Mol Biotechnol 2018 Nov;60(11):773-782

Istituto Superiore di Sanità (ISS), National Center for Global Health, Viale Regina Elena 299, 00161, Rome, Italy.

Eukaryotic cells constitutively produce nanovesicles of 50-150 nm of diameter, referred to as exosomes, upon release of the contents of multivesicular bodies (MVBs). We recently characterized a novel, exosome-based way to induce cytotoxic T lymphocyte (CTL) immunization against full-length antigens. It is based on DNA vectors expressing products of fusion between the exosome-anchoring protein Nef mutant (Nef) with the antigen of interest. Read More

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http://dx.doi.org/10.1007/s12033-018-0114-3DOI Listing
November 2018
11 Reads
1.880 Impact Factor

Easy In Vitro Synthesis of Optimised Functioning Reporter mRNA from Common eGFP Plasmid.

Mol Biotechnol 2018 Oct;60(10):762-771

EMBRAPA (Brazilian Agricultural Research Corporation), Av. Eugênio do Nascimento, 610 - Aeroporto, Juiz de Fora, MG, 36038-330, Brazil.

The extensive growth in number and importance of experiments and clinical-aimed techniques based solely or majorly on the activity of RNA strands, e.g. CRSPR/Cas9 and siRNA, has put emphasis on the necessity of standardisation of experiments with RNA. Read More

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http://dx.doi.org/10.1007/s12033-018-0112-5DOI Listing
October 2018
24 Reads