2,099 results match your criteria Molecular biotechnology[Journal]


Use of the ptxD gene as a portable selectable marker for chloroplast transformation in Chlamydomonas reinhardtii.

Mol Biotechnol 2019 Apr 17. Epub 2019 Apr 17.

Instituto Politécnico Nacional, Unidad Profesional Interdisciplinaria de Biotecnología, Av. Acueducto SN, Col. Barrio la Laguna Ticomán, 07340, Mexico City, Mexico.

Synthetic biology and genetic engineering in algae offer an unprecedented opportunity to develop species with traits that can help solve the problems associated with food and energy supply in the 21st century. In the green alga Chlamydomonas reinhardtii, foreign genes can be expressed from the chloroplast genome for molecular farming and metabolic engineering to obtain commodities and high-value molecules. To introduce these genes, selectable markers, which rely mostly on the use of antibiotics, are needed. Read More

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http://dx.doi.org/10.1007/s12033-019-00177-3DOI Listing

Optimizing Periplasmic Expression in Escherichia coli for the Production of Recombinant Proteins Tagged with the Small Metal-Binding Protein SmbP.

Mol Biotechnol 2019 Apr 17. Epub 2019 Apr 17.

Universidad Autonoma de Nuevo Leon, Facultad de Ciencias Quimicas, 66455, San Nicolas de los Garza, NL, Mexico.

We have previously shown that the small metal-binding protein (SmbP) extracted from the gram-negative bacterium Nitrosomonas europaea can be employed as a fusion protein for the expression and purification of recombinant proteins in Escherichia coli. With the goal of increasing the amounts of SmbP-tagged proteins produced in the E. coli periplasm, we replaced the native SmbP signal peptide with three different signal sequences: two were from the proteins CusF and PelB, for transport via the Sec pathway, and one was the signal peptide from TorA, for transport via the Tat pathway. Read More

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http://dx.doi.org/10.1007/s12033-019-00176-4DOI Listing

Analysis of Arabidopsis thaliana HKT1 and Eutrema salsugineum/botschantzevii HKT1;2 Promoters in Response to Salt Stress in Athkt1:1 Mutant.

Mol Biotechnol 2019 Apr 12. Epub 2019 Apr 12.

Department of Genetics, Faculty of Earth and Life Sciences, Vrije Universiteit, De Boelelaan 1085, 1081 HV, Amsterdam, The Netherlands.

Soil salinity imposes a serious threat to the productivity of agricultural crops. Among several other transporters, high-affinity K transporter (HKT)'s play an important role in reducing the phytotoxicity of Na. Expression of Eutrema salsugineum (a halophyte) HKT1;2 is induced upon salt exposure. Read More

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http://dx.doi.org/10.1007/s12033-019-00175-5DOI Listing

High Conversion of D-Fructose into D-Allulose by Enzymes Coupling with an ATP Regeneration System.

Mol Biotechnol 2019 Apr 8. Epub 2019 Apr 8.

School of Life Sciences and Chemical Technology, Ngee Ann Polytechnic, Singapore, 599489, Singapore.

D-Allulose is a rare monosaccharide that exists in extremely small quantities in nature, and it is also hard to prepare at a large scale via chemical or enzyme synthetic route due to low conversion and downstream separation complexity. Using D-psicose epimerase and L-rhamnulose kinase, a method enabling high conversion of D-allulose from D-fructose without the need for a tedious isomer separation step was established recently. However, this method requires expensive ATP to facilitate the reaction. Read More

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http://dx.doi.org/10.1007/s12033-019-00174-6DOI Listing

A High-Throughput Platform for the Generation of Synthetic Ab Clones by Single-Strand Site-Directed Mutagenesis.

Authors:
Eugenio Gallo

Mol Biotechnol 2019 Apr 8. Epub 2019 Apr 8.

Department of Molecular Genetics, Charles Best Institute, University of Toronto, 112 College Street, Room 70, Toronto, ON, M5G 1L6, Canada.

Current developments in meta-data analysis and predictive computational models offer alternative routes for the identification of antibodies. In silico-based technologies and NGS data analysis from Ab phage-display selections offer expanded selections of Ab candidates. Accordingly, the identified de novo Abs with predicted selectivity for a target antigen must undergo rapid gene synthesis for downstream Ab characterizations. Read More

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http://dx.doi.org/10.1007/s12033-019-00171-9DOI Listing

Establishment and Characterization of a Novel Tissue-specific DNA Construct and Culture System with Potential for Avian Bioreactor Generation.

Mol Biotechnol 2019 Apr 3. Epub 2019 Apr 3.

Instituto de Biología y Medicina Experimental-CONICET, Vuelta de Obligado 2490, 1428, Buenos Aires, Argentina.

Transgenic chickens are of great interest for the production of recombinant proteins in their eggs. However, the use of constitutive strong promoters or the tissue-specific ovalbumin promoter for the generation of the transgenic chickens have different drawbacks that have to be overcome in order to make chicken bioreactor an efficient production system. This prompted us to investigate the use of an alternative tissue-specific promoter, the vitellogenin promoter, which could overcome the difficulties currently found in the generation of chicken bioreactors. Read More

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http://dx.doi.org/10.1007/s12033-019-00170-wDOI Listing
April 2019
1 Read

Molecular Cloning and Transient Expression of Recombinant Human PPARγ in HEK293T Cells Under an Inducible Tet-on System.

Mol Biotechnol 2019 Apr 2. Epub 2019 Apr 2.

Universidad Autonoma de Nuevo Leon, Genetic Engineering and Genomics Laboratory, School of Chemistry, San Nicolas de los Garza, Nuevo Leon, Mexico.

Peroxisome proliferator-activated receptor gamma (PPARγ) is involved in the regulation of lipid and glucose homeostasis and inflammation. PPARγ expression level has been widely studied in multiple tissues; however, there are few reports of preceding attempts to produce full-length human PPARγ (hPPARγ) in cellular models, and generally, expression level is not known or measurable. We propose an alternative strategy to express recombinant hPPARγ1, using a transient transfection with an inducible Tet-On 3G system where target and reporter gene were cloned in the same open reading frame. Read More

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http://dx.doi.org/10.1007/s12033-019-00173-7DOI Listing
April 2019
1 Read

Members of B-box Protein Family from Malus domestica Enhanced Abiotic Stresses Tolerance in Escherichia coli.

Mol Biotechnol 2019 Apr 1. Epub 2019 Apr 1.

College of Life Science, State Key Laboratory of Crop Biology, Shandong Agricultural University, Tai'an, 271018, Shandong, People's Republic of China.

The B-box proteins (BBXs) are zinc finger proteins containing one or two B-box domain(s) and involved in regulation of development processes as transcription factors in plants. Here, seven BBX genes in Malus domestica genome (MdBBXs) were identified and found to be up-regulated under abiotic stresses, with 2-12 folds in roots. All recombinant MdBBXs expressed in Escherichia coli (E. Read More

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http://dx.doi.org/10.1007/s12033-019-00172-8DOI Listing
April 2019
6 Reads
1.876 Impact Factor

A Novel Mini Protein Design of Haloalkane Dehalogenase.

Mol Biotechnol 2019 Mar 27. Epub 2019 Mar 27.

Enzyme and Microbial Research Centre, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 UPM, Serdang, Selangor, Malaysia.

The application of native enzymes may not be economical owing to the stability factor. A smaller protein molecule may be less susceptible to external stresses. Haloalkane dehalogenases (HLDs) that act on toxic haloalkanes may be incorporated as bioreceptors to detect haloalkane contaminants. Read More

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http://dx.doi.org/10.1007/s12033-019-00169-3DOI Listing
March 2019
1 Read

Biochemical Characterization of Heat-Tolerant Recombinant L-Arabinose Isomerase from Enterococcus faecium DBFIQ E36 Strain with Feasible Applications in D-Tagatose Production.

Mol Biotechnol 2019 Mar 27. Epub 2019 Mar 27.

Grupo de Ingeniería de Alimentos y Biotecnología, Instituto de Desarrollo Tecnológico para la Industria Química, Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Universidad Nacional del Litoral (UNL), Colectora RN 168 Km 472 "Paraje El Pozo" S/N, (S3000GLN), Santa Fe, Argentina.

D-Tagatose is a ketohexose, which presents unique properties as a low-calorie functional sweetener possessing a sweet flavor profile similar to D-sucrose and having no aftertaste. Considered a generally recognized as safe (GRAS) substance by FAO/WHO, D-tagatose can be used as an intermediate for the synthesis of other optically active compounds as well as an additive in detergent, cosmetic, and pharmaceutical formulations. This study reports important features for L-arabinose isomerase (EC 5. Read More

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http://link.springer.com/10.1007/s12033-019-00161-x
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http://dx.doi.org/10.1007/s12033-019-00161-xDOI Listing
March 2019
21 Reads

Electrospun Poly-ε-Caprolactone (PCL)/Dicalcium Phosphate Dihydrate (DCPD) Composite Scaffold for Tissue Engineering Application.

Mol Biotechnol 2019 May;61(5):345-354

Department of Textile Engineering, Isfahan University of Technology, Isfahan, Iran.

Recently electrospun scaffolds show excellent response in cell adhesion, growth, and tissue healing in comparison with other techniques. So in this study, PCL and PCL/DCPD scaffolds were designed and prepared with electrospinning. The electrospun scaffolds were characterized by scanning electron microscope with X-ray elemental analysis, atomic force microcopy, differential scanning calorimetry, and contact angle analysis for optimizing the effective parameters. Read More

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http://dx.doi.org/10.1007/s12033-019-00168-4DOI Listing

A Modified In Vitro Transcription Approach to Improve RNA Synthesis and Ribozyme Cleavage Efficiency.

Mol Biotechnol 2019 Mar 13. Epub 2019 Mar 13.

Key Laboratory of Science and Technology of Eco-Textiles, Ministry of Education, College of Chemistry, Chemical Engineering and Biotechnology, DongHua University, 2999 North Ren Min Road, Shanghai, 201620, China.

RNA elements such as catalytic RNA, riboswitch, microRNA, and long non-coding RNA perform a major role in cellular processes. A complete understanding of cellular processes is impossible without knowing the structure-function relationship of participating RNA molecules that ultimately requires large quantities of pure RNAs. Thus, structural/functional analyses of emerging RNAs necessitate revised protocols for improved RNA quantity and quality. Read More

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http://dx.doi.org/10.1007/s12033-019-00167-5DOI Listing
March 2019
2 Reads

Isopentenyl Transferase (IPT) Gene Transfer to Perennial Ryegrass Through Sonication-Assisted Agrobacterium-Mediated Transformation (SAAT), Vacuum and Heat Treatment.

Mol Biotechnol 2019 May;61(5):332-344

Institute of Biotechnology, Shiraz University, Shiraz, Iran.

The successful introduction of isopentenyl transferase (IPT) gene into perennial ryegrass, cultivars Numan and Grassland using Agrobacterium tumefaciens via three explants (callus, seed and meristem tip) under three individual experiment was evaluated. In the first experiment, the calli were inoculated with LBA4404 Agrobacterium strain under vacuum, heat and in combination of both at 42 °C for 5 min followed by vacuum treatment (390 mm Hg pressure) for 15 min. Sonication-assisted Agrobacterium-mediated transformation (SAAT) was applied for seed and meristem tip transformation of perennial ryegrass for the first time. Read More

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http://dx.doi.org/10.1007/s12033-019-00165-7DOI Listing
May 2019
6 Reads

Yeast Expression Systems: Overview and Recent Advances.

Mol Biotechnol 2019 May;61(5):365-384

Biotechnology Research Center, Tabriz University of Medical Sciences, Daneshgah Ave, Tabriz, Iran.

Yeasts are outstanding hosts for the production of functional recombinant proteins with industrial or medical applications. Great attention has been emerged on yeast due to the inherent advantages and new developments in this host cell. For the production of each specific product, the most appropriate expression system should be identified and optimized both on the genetic and fermentation levels, considering the features of the host, vector and expression strategies. Read More

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http://dx.doi.org/10.1007/s12033-019-00164-8DOI Listing
May 2019
2 Reads

A High Efficient Method to Isolate Exosomes from Small Intestinal Epithelium.

Mol Biotechnol 2019 May;61(5):325-331

Division of Geriatric Endocrinology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, 210029, China.

The intestinal epithelium is an important receptor that is not only exposed to nutrients but also to pathogens, such as ingested toxins, bacterial flora, and their metabolites. The sensory information is communicated to extensive endocrine, neural, immune systems, and the exosomes acts as carriers of communication from cell-to-cell. Isolation of exosomes from small intestinal epithelium remains more complex to obtain as a source of exosomes, as it contains varying proportions of exosomes derived from many different cells. Read More

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http://dx.doi.org/10.1007/s12033-019-00163-9DOI Listing

HEK293 Cells Overexpressing Nuclear Factor E2-Related Factor-2 Improve Expression of Recombinant Coagulation Factor VII.

Mol Biotechnol 2019 May;61(5):317-324

Stem cell and Regenerative Medicine Research Center, Guilan University of Medical Sciences, Rasht, Iran.

The mammalian expression system plays a key central role in the production of therapeutic recombinant proteins. Conspicuously, any improvements in the expression system which lead to a higher expression level would have an impact especially in bio-pharmaceutical industries. In the current study, to take steps toward the improvement of expression of recombinant protein, first, we established a stable HEK293 cell line to overexpress a well-known cytoprotective and antioxidant gene, Nrf2. Read More

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http://dx.doi.org/10.1007/s12033-019-00160-yDOI Listing
May 2019
1 Read

Biochemical, Kinetic, and Computational Structural Characterization of Shikimate Kinase from Methicillin-Resistant Staphylococcus aureus.

Mol Biotechnol 2019 Apr;61(4):274-285

Facultad de Medicina y Nutrición, Universidad Juárez del Estado de Durango, Av. Universidad y Fanny Anitua S/N, C.P. 34000, Durango, Dgo, Mexico.

One of the most widespread pathogens worldwide is methicillin-resistant Staphylococcus aureus, a bacterium that provokes severe life-threatening illnesses both in hospitals and in the community. The principal challenge lies in the resistance of MRSA to current treatments, which encourages the study of different molecular targets that could be used to develop new drugs against this infectious agent. With this goal, a detailed characterization of shikimate kinase from this microorganism (SaSK) is described. Read More

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http://link.springer.com/10.1007/s12033-019-00159-5
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http://dx.doi.org/10.1007/s12033-019-00159-5DOI Listing
April 2019
15 Reads
1.876 Impact Factor

Correction to: Production of a Recombinant Dermaseptin Peptide in Nicotiana tabacum Hairy Roots with Enhanced Antimicrobial Activity.

Mol Biotechnol 2019 Apr;61(4):253

Department of Agricultural Biotechnology, Faculty of Agricultural Sciences, University of Guilan, Rasht, 4199613776, Iran.

The original version of this article unfortunately contained a mistake in the unit "μg/l". The unit "μg/l" should be corrected to "μg/ml" throughout the paper. Read More

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http://link.springer.com/10.1007/s12033-019-00157-7
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http://dx.doi.org/10.1007/s12033-019-00157-7DOI Listing
April 2019
7 Reads

Responsiveness and Adaptation to Salt Stress of the REDOX-RESPONSIVE TRANSCRIPTION FACTOR 1 (RRTF1) Gene are Controlled by its Promoter.

Mol Biotechnol 2019 Apr;61(4):254-260

Center for Plant Sciences, University of Leeds, Leeds, LS2 9JT, UK.

The REDOX-RESPONSIVE TRANSCRIPTION FACTOR 1 (RRTF1) gene encodes a member of the ERF/AP2 transcription factor family involved in redox homeostasis. The RRTF1 gene shows tissue-specific responsiveness to various abiotic stress treatments including a response to salt stress in roots. An interesting feature of this response is an adaptation phase that follows its activation, when promoter levels revert to a base line level, even if salt stress is maintained. Read More

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http://dx.doi.org/10.1007/s12033-019-00155-9DOI Listing
April 2019
7 Reads

Analysis of In Vivo Activity of the Bovine Cholesterol Hydroxylase/Lyase System Proteins Expressed in Escherichia coli.

Mol Biotechnol 2019 Apr;61(4):261-273

A.N. Belozersky Institute of Physico-Chemical Biology, M.V. Lomonosov Moscow State University, Leninskie Gory, 1/40, Moscow, 119234, Russia.

The cholesterol hydroxylase/lyase (CHL) system, located in the mitochondria of the mammalian adrenal cortex cells, consists of cytochrome P450scc (CYP11A1), adrenodoxin (Adx), and adrenodoxin reductase (AdR) and performs the first stage of the steroidogenesis: AdR and Adx enable the electron transfer between NADPH and cytochrome P450scc, and P450scc catalyzes the conversion of cholesterol into pregnenolone. CHL system was reconstructed in Escherichia coli using the polycistronic plasmid pTrc99A/CHL. In E. Read More

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http://dx.doi.org/10.1007/s12033-019-00158-6DOI Listing

Phage Display Libraries: From Binders to Targeted Drug Delivery and Human Therapeutics.

Authors:
Mouldy Sioud

Mol Biotechnol 2019 Apr;61(4):286-303

Department of Cancer Immunology, Oslo University Hospital, The Norwegian Radium Hospital, Montebello, 0310, Oslo, Norway.

The application of repertoire selection technologies for the study and characterization of tumor heterogeneity is an area of great interest in the field of tumor biology and immunotherapy. Among the most promising approaches, phage display has been successfully used to select peptides and antibody fragments to a variety of different targets, including cancer cells, immune cells and cytokines. Peptides selected from phage display have been used to guide the delivery of lytic peptides, cytotoxic drugs, and nanoparticles to cancer cells with the aim to obtain more efficient and less toxic therapeutics. Read More

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http://dx.doi.org/10.1007/s12033-019-00156-8DOI Listing
April 2019
14 Reads

Construction and Characterization of a Medium Copy Number Expression Vector Carrying Auto-Inducible dps Promoter to Overproduce a Bacterial Superoxide Dismutase in Escherichia coli.

Mol Biotechnol 2019 Apr;61(4):231-240

Laboratory of Pharmaceutical Biotechnology, School of Pharmacy, Institut Teknologi Bandung, Bandung, 40132, Indonesia.

Medium copy number expression vector and auto-inducible promoter could be a solution for producing recombinant therapeutic proteins in industrial scale regarding plasmid stability, cost, and product quality. This work aimed to construct a medium copy number pBR322-based expression vector carrying auto-inducible promoter, determine its ability to express heterologous gene, and study its segregational stability. Three stationary-phase promoters of Escherichia coli genes (gadA, dps and sbmC) were used to produce a superoxide dismutase from Staphylococcus equorum (rMnSODSeq) coding region from pBR322Δtet (pBR322-mini). Read More

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http://dx.doi.org/10.1007/s12033-018-00151-5DOI Listing

The Growth Differentiation Factor 11 is Involved in Skin Fibroblast Ageing and is Induced by a Preparation of Peptides and Sugars Derived from Plant Cell Cultures.

Mol Biotechnol 2019 Mar;61(3):209-220

Arterra Bioscience srl, via B. Brin 69, 80142, Napoli, Italy.

Ageing is a complex and progressive phenomenon, during which the accumulation of morphological and chemical changes seriously compromises the capacity of the cells to proliferate and fulfil their biological tasks. The increase in the average age of the world population, associated with a higher occurrence of age-related diseases, is prompting scientific research to look for new strategies and molecular targets that may help in alleviating age-related phenotypes. Growth factors, responsible for modulating several aging markers in many tissues and organs, represent valuable targets to fight age-associated dysfunctions. Read More

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http://link.springer.com/10.1007/s12033-019-00154-w
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http://dx.doi.org/10.1007/s12033-019-00154-wDOI Listing
March 2019
8 Reads

Validated Nuclear-Based Transgene Expression Regulated by the Fea1 Iron-Responsive Promoter in the Green Alga Chlamydomonas reinhardtii.

Mol Biotechnol 2019 May;61(5):305-316

Institute of Bioprocess Engineering, Friedrich-Alexander-Universität Erlangen-Nürnberg, Paul-Gordan-Strasse 3, 91052, Erlangen, Germany.

Microalgae are in the focus for the production of recombinant proteins in research and potential commercial application. Inducible promoters represent important tools that potentially allow the expression of recombinant proteins at higher rates. In general, they are used to separate the culture growth phase from the production phase by initiating product formation after high cell densities have been achieved. Read More

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http://dx.doi.org/10.1007/s12033-018-00148-0DOI Listing
May 2019
1 Read

Building Bridges Between Structural and Network-Based Systems Biology.

Mol Biotechnol 2019 Mar;61(3):221-229

Institute of Chemical Engineering Sciences, Foundation for Research & Technology-Hellas (FORTH/ICE-HT), 26504, Patras, Greece.

The integration of structural and network-based systems biology is paramount for the improved understanding of how the proteins interact to modulate the behavior of complex biological systems. This review presents the current literature on the computational studies that combine these two scientific fields focusing on two main approaches: network-based analysis of the structure and dynamics of proteins and the in silico reconstruction of protein-protein interaction (PPI) networks or expansion of existed protein interactomes driven by structural annotations. Last, to enrich the current knowledge of the topological properties of the protein structure networks and evaluate the capacity of the public structural annotations of protein domains to predict novel PPIs, further computational analyses, missing so far from the literature, were performed. Read More

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http://dx.doi.org/10.1007/s12033-018-0146-8DOI Listing

Production of a Recombinant Dermaseptin Peptide in Nicotiana tabacum Hairy Roots with Enhanced Antimicrobial Activity.

Mol Biotechnol 2019 Apr;61(4):241-252

Department of Agricultural Biotechnology, Faculty of Agricultural Sciences, University of Guilan, Rasht, 4199613776, Iran.

Expression of strong antimicrobial peptides in plants is of great interest to combat a wide range of plant pathogens. To bring the Dermaseptin B1 (DrsB1) peptide to the intimate contact of the plant pathogens cell wall surface, the DrsB1 encoding sequence was fused to the C-terminal part of the two copies of the chitin-binding domain (CBD) of the Avr4 effector protein and used for Agrobacterium rhizogenes-mediated transformation. The expression of the recombinant protein in the tobacco hairy roots (HRs) was confirmed by molecular analysis. Read More

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http://link.springer.com/10.1007/s12033-019-00153-x
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http://dx.doi.org/10.1007/s12033-019-00153-xDOI Listing
April 2019
16 Reads

Isolation and Expression Analysis of Three Types of α-Carbonic Anhydrases from the Antarctic Alga Chlamydomonas sp. ICE-L under Different Light Stress Treatments.

Mol Biotechnol 2019 Mar;61(3):200-208

College of Chemical Engineering, Qingdao University of Science and Technology, Qingdao, 266042, China.

Carbonic anhydrases (CAs) are a class of zinc-containing metalloenzymes that can reversibly catalyse the hydration reaction of carbon dioxide. Antarctic algae are the most critical component of the Antarctic ecosystem; algae can enter the carbon cycle food chain by fixing carbon dioxide from the air. In this study, the complete open reading frames (ORFs) of CA1 (GenBank ID KY826431), CA2 (GenBank ID KY826432), and CA3 (GenBank ID KY826433), encoding CAs in the Antarctic ice microalga Chlamydomonas. Read More

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http://link.springer.com/10.1007/s12033-018-00152-4
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http://dx.doi.org/10.1007/s12033-018-00152-4DOI Listing
March 2019
5 Reads

CRISPR/Cas9-Mediated Multiply Targeted Mutagenesis in Orange and Purple Carrot Plants.

Mol Biotechnol 2019 Mar;61(3):191-199

State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of Horticulture, Nanjing Agricultural University, Nanjing, 210095, China.

The clustered regularly interspaced short palindromic repeats (CRISPR)-associated protein 9 (Cas9) system has been successfully used for precise genome editing in many plant species, including in carrot cells, very recently. However, no stable gene-editing carrot plants were obtained with CRISPR/Cas9 system to date. In the present study, four sgRNA expression cassettes, individually driven by four different promoters and assembled in a single CRISPR/Cas9 vector, were transformed into carrots using Agrobacterium-mediated genetic transformation. Read More

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http://dx.doi.org/10.1007/s12033-018-00150-6DOI Listing

Understanding mA Function Through Uncovering the Diversity Roles of YTH Domain-Containing Proteins.

Mol Biotechnol 2019 May;61(5):355-364

College of Animal Sciences, Zhejiang University, No. 866 Yuhangtang Road, Hangzhou, 310058, Zhejiang, China.

N-methyladenosine (mA) is the most abundant-internal modification of eukaryotic mRNA. mA can be installed and removed by specific enzymes. The "writer," "eraser," and "reader" of mA modification have been reported. Read More

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http://dx.doi.org/10.1007/s12033-018-00149-zDOI Listing

Development of Soybean Yellow Mottle Mosaic Virus-Based Expression Vector for Heterologous Protein Expression in French Bean.

Mol Biotechnol 2019 Mar;61(3):181-190

Advanced Centre for Plant Virology, Division of Plant Pathology, Indian Agricultural Research Institute, New Delhi, 110012, India.

Plant virus-based vectors provide attractive and valuable tools for rapid production of recombinant protein in large quantities as they produce systemic infections in differentiated plant tissues. In the present study, we engineered the Soybean yellow mottle mosaic virus (SYMMV) as a gene expression vector which is a promising candidate for systemic expression of foreign proteins in French bean plants. Full virus vector strategy was exploited for insertion of foreign gene by inserting MCS through PCR in the circular pJET-SYMMV clone. Read More

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http://dx.doi.org/10.1007/s12033-018-0147-7DOI Listing
March 2019
1 Read
1.876 Impact Factor

Ethylene Response Factor (ERF) Family Proteins in Abiotic Stresses and CRISPR-Cas9 Genome Editing of ERFs for Multiple Abiotic Stress Tolerance in Crop Plants: A Review.

Mol Biotechnol 2019 Feb;61(2):153-172

Biotechnology Group, Biological Sciences and Technology Division, CSIR-NEIST, Jorhat, Assam, 785006, India.

Abiotic stresses such as extreme heat, cold, drought, and salt have brought alteration in plant growth and development, threatening crop yield and quality leading to global food insecurity. Many factors plays crucial role in regulating various plant growth and developmental processes during abiotic stresses. Ethylene response factors (ERFs) are AP2/ERF superfamily proteins belonging to the largest family of transcription factors known to participate during multiple abiotic stress tolerance such as salt, drought, heat, and cold with well-conserved DNA-binding domain. Read More

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http://dx.doi.org/10.1007/s12033-018-0144-xDOI Listing
February 2019
1 Read
1.876 Impact Factor

Improved Delivery of CRISPR/Cas9 System Using Magnetic Nanoparticles into Porcine Fibroblast.

Mol Biotechnol 2019 Mar;61(3):173-180

Department of Biochemistry and Biotechnology, Poznan University of Life Sciences, Dojazd 11, 60-632, Poznan, Poland.

Genetically modified pigs play an important role in agriculture and biomedical research; hence, new efficient methods are needed to obtain genetically engineered cells and animals. The clustered regularly interspaced short palindromic repeats (CRISPR)/Cas (CRISPR-associated) system represents an effective genome editing tool. It consists of two key molecules: single guide RNA (sgRNA) and the Cas9 endonuclease that can be introduced into the cells as one plasmid. Read More

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http://dx.doi.org/10.1007/s12033-018-0145-9DOI Listing
March 2019
4 Reads

Engineering Chlamydomonas reinhardtii for Expression of Functionally Active Human Interferon-α.

Mol Biotechnol 2019 Feb;61(2):134-144

Biology Department, Faculty of Science Yanbu, Taibah University, King Khalid Rd, Al amoedi, Yanbu El-Bahr, 46423, Saudi Arabia.

Human interferon (IFN) are secreted cytokines that play a major regulatory role in response to various infections. Commercially, IFN-α has been approved to treat many chronic viral diseases as well as a variety of cancers and different types of leukemia. In this study, a binary vector containing human IFN-α2a gene under the regulation of the cauliflower mosaic virus 35S promoter was constructed. Read More

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http://link.springer.com/10.1007/s12033-018-0143-y
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http://dx.doi.org/10.1007/s12033-018-0143-yDOI Listing
February 2019
3 Reads

New Nuclease from Extremely Psychrophilic Microorganism Psychromonas ingrahamii 37: Identification and Characterization.

Mol Biotechnol 2019 Feb;61(2):122-133

Department of Biomedical Chemistry, Faculty of Chemistry, University of Gdańsk, ul. Wita Stwosza 63, 80-308, Gdańsk, Poland.

Nucleases are an important group of hydrolases that degrade nucleic acids, with broad spectrum of applications in science and industry. In this paper, we report the identification and characterization of the nuclease from extremely psychrophilic bacterium Psychromonas ingrahamii that grows exponentially at 5 °C, but may also grow at even lower temperatures (down to - 12 °C). The putative endonuclease I gene, identified in the genome of P. Read More

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http://dx.doi.org/10.1007/s12033-018-0142-zDOI Listing
February 2019
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Generation and Characterization of Fibroblast-Specific Basigin Knockout Mice.

Mol Biotechnol 2019 Feb;61(2):111-121

Department of Cell Biology, National Translational Science Center for Molecular Medicine, State Key Laboratory of Cancer Biology, Fourth Military Medical University, No. 169 Changle West Road, Xi'an, 710032, China.

Basigin is a well-known extracellular stimulator of fibroblasts and may confer resistance to apoptosis of fibroblasts in vitro under some pathological status, but its exact function in fibroblasts and the underlying mechanism remain poorly understood. The systematic Basigin gene knockout leads to the perinatal lethality of mice, which limits the delineation of its function in vivo. In this study, we generated a fibroblast-specific Basigin knock-out mouse model and demonstrated the successful deletion of Basigin in fibroblasts. Read More

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http://dx.doi.org/10.1007/s12033-018-0141-0DOI Listing
February 2019
2 Reads

Expression of Recombinant PfCelTOS Antigen in the Chloroplast of Chlamydomonas reinhardtii and its Potential Use in Detection of Malaria.

Mol Biotechnol 2019 Feb;61(2):102-110

Department of Biotechnology, Iranian Research Organization for Science and Technology, P.O. BOX: 3353-51111, Tehran, Iran.

Malaria is a serious but preventable and treatable infectious disease that is found in over 100 countries around the world. Correct and rapid diagnosis of malaria infection can rescue the patient of getting sicker and reduces the risk of disease spreading among humans. Chlamydomonas reinhardtii chloroplast is an attractive platform for expressing malaria antigens because it is capable of folding complex proteins, including those requiring disulfide bond formation, while lack the ability to glycosylate proteins; a valuable quality of any malaria protein expression system, since the Plasmodium parasite lacks N-linked glycosylation machinery. Read More

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http://link.springer.com/10.1007/s12033-018-0140-1
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http://dx.doi.org/10.1007/s12033-018-0140-1DOI Listing
February 2019
1 Read
1.876 Impact Factor

The Effect of Methyl Jasmonate and Temperature on the Transient Expression of Recombinant Proteins in Cucurbita pepo L.

Mol Biotechnol 2019 Feb;61(2):84-92

Department of Biotechnology, Faculty of Agriculture, Tarbiat Modares University, Tehran, Iran.

The aim of this study is to assess the effect of methyl jasmonate (MeJA) and temperature on the valuable pharmaceuticals expression in a virus-mediated transient expression system, and so the Zuchini Yellow Mosaic Virus (ZYMV) based vector was used for transferring the GFP reporter gene and recombinant tissue plasminogen activator (rtPA) gene (K2S) to cucurbit (Cucurbita pepo L.). MeJA, temperature and time (days after inoculation), were evaluated as a factorial experiment in a completely randomized design (CRD). Read More

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http://dx.doi.org/10.1007/s12033-018-0138-8DOI Listing
February 2019
1.876 Impact Factor

Strategies for the Construction of Cassava Brown Streak Disease Viral Infectious Clones.

Mol Biotechnol 2019 Feb;61(2):93-101

School of Biological Sciences, University of Bristol, 24 Tyndall Ave, Bristol, BS8 1TQ, UK.

Cassava brown streak disease (CBSD) has major impacts on yield and quality of the tuberous roots of cassava in Eastern and Central Arica. At least two Potyviridae species cause the disease: Cassava brown streak virus (CBSV) and Ugandan cassava brown streak virus (UCBSV). Cloned viral genome sequences known as infectious clones (ICs) have been important in the study of other viruses, both as a means of standardising infectious material and characterising viral gene function. Read More

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http://link.springer.com/10.1007/s12033-018-0139-7
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http://dx.doi.org/10.1007/s12033-018-0139-7DOI Listing
February 2019
16 Reads

Oxidative Damages to Eye Stem Cells, in Response to, Bright and Ultraviolet Light, Their Associated Mechanisms, and Salvage Pathways.

Mol Biotechnol 2019 Feb;61(2):145-152

Stem Cells and Regenerative Medicine Centre, Yenepoya Research Centre, Yenepoya (Deemed to be University), University Road, Derlakatte, Mangalore, Karnataka, 575018, India.

The surface of the eye is continuously exposed to the harshness of the external environment. As a homeostatic mechanism for replenishing the worn off cells of the ocular surface, a balance is maintained via the role of ocular stem cells. However, under extreme conditions of harshness like exposure to bright and ultraviolet light, the ocular stem cells are unable to do the repair mechanisms resulting in severe impairment of vision and disturbances in the eye. Read More

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http://link.springer.com/10.1007/s12033-018-0136-x
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http://dx.doi.org/10.1007/s12033-018-0136-xDOI Listing
February 2019
7 Reads
1.876 Impact Factor

DNA Vaccine Targeting Gonadotropin-Releasing Hormone Receptor and Its Application in Animal Contraception.

Mol Biotechnol 2019 Feb;61(2):73-83

Scott-Ritchey Research Center, College of Veterinary Medicine, Auburn University, 1265 HC Morgan Drive, Auburn, AL, 36849, USA.

Overpopulation of free-roaming and wildlife animals negatively affects economy and public health in many parts of the world. Contraceptive vaccines are viewed as a valuable option for reducing numbers of unwanted animals. This study develops vaccines for potential use in animal contraception exploiting a DNA platform. Read More

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http://link.springer.com/10.1007/s12033-018-0137-9
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http://dx.doi.org/10.1007/s12033-018-0137-9DOI Listing
February 2019
16 Reads

The Constitutive Expression of a Chrysanthemum ERF Transcription Factor Influences Flowering Time in Arabidopsis thaliana.

Mol Biotechnol 2019 Jan;61(1):20-31

Key Laboratory of Landscape Agriculture, College of Horticulture, Ministry of Agriculture, Nanjing Agricultural University, Weigang No. 1, Nanjing, 210095, Jiangsu, Republic of China.

AP2/ERF transcription factors (TFs) represent valuable targets for the genetic manipulation of crop plants, as they participate in the control of metabolism, growth and development, as well as in the plants' response to environmental stimuli. Here, an ERF TF encoded by the chrysanthemum (Chrysanthemum morifolium) genome, designated CmERF110, was cloned and functionally characterized. The predicted CmERF110 polypeptide included a conserved DNA-binding AP2/ERF domain. Read More

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http://dx.doi.org/10.1007/s12033-018-0134-zDOI Listing
January 2019

Effect of C-Terminus Modification in Salmonella typhimurium FliC on Protein Purification Efficacy and Bioactivity.

Mol Biotechnol 2019 Jan;61(1):12-19

Department of Immunology, Razi Vaccine and Serum Research Institute (RVSRI), Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran.

Recombinant flagellin (FliC) has shown low efficacy in purification because of inclusion bodies formation and aggregation. We hypothesized preserving TLR5 binding site of FliC and removing some amino acids could be responsible for aggregation and solubility improvement. Hence, a bioinformatics study was performed to find hotspots in aggregate formation. Read More

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http://dx.doi.org/10.1007/s12033-018-0135-yDOI Listing
January 2019
22 Reads

Ribosome Display: A Potent Display Technology used for Selecting and Evolving Specific Binders with Desired Properties.

Mol Biotechnol 2019 Jan;61(1):60-71

Department of Biochemical Engineering, School of Chemical Engineering & Technology, Tianjin University, Tianjin, 300350, People's Republic of China.

Ribosome display is a powerful engineering research tool for the high-throughput selection of peptides or proteins, which results in the generation of high-performance binders against nearly any antigen of interest. As a cell-free display system, ribosome display has been well developed with many outstanding achievements for over 20 years. Compared with other related display techniques, ribosome display shows unique advantages and development prospects. Read More

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http://dx.doi.org/10.1007/s12033-018-0133-0DOI Listing
January 2019

In-Silico Bioprospecting: Finding Better Enzymes.

Mol Biotechnol 2019 Jan;61(1):53-59

Department of Biological Sciences, Sunandan Divatia School of Science, NMIMS Deemed to be University, Vile Parle (W), Mumbai, 400056, India.

Enzymes are essential biological macromolecules, which catalyse chemical reactions and have impacted the human civilization tremendously. The importance of enzymes as biocatalyst was realized more than a century ago by eminent scientists like Kuhne, Buchner, Payen, Sumner, and the last three decades has seen exponential growth in enzyme industry, mainly due to the revolution in tools and techniques in molecular biology, biochemistry and production. This has resulted in high demand of enzymes in various applications like food, agriculture, chemicals, pharmaceuticals, cosmetics, environment and research sector. Read More

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http://link.springer.com/10.1007/s12033-018-0132-1
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http://dx.doi.org/10.1007/s12033-018-0132-1DOI Listing
January 2019
10 Reads

Correction to: Efficient Differentiation of Human Embryonic Stem Cells Toward Dopaminergic Neurons Using Recombinant LMX1A Factor.

Mol Biotechnol 2019 Jan;61(1):72

Department of Developmental Biology, University of Science and Culture, ACECR, Tehran, Iran.

The original version of this article was published without article note. The article note is given below. Read More

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http://dx.doi.org/10.1007/s12033-018-0131-2DOI Listing
January 2019
1 Read
1.880 Impact Factor

Ferulic Acid Produced by Lactobacillus fermentum Influences Developmental Growth Through a dTOR-Mediated Mechanism.

Mol Biotechnol 2019 Jan;61(1):1-11

Biomedical Technology and Cell Therapy Research Laboratory, Department of Biomedical Engineering, Faculty of Medicine, McGill University, 3775 University Street, Montreal, QC, H3A2B4, Canada.

The composition and activity of the gut microbiota impacts several energy-regulating conditions including diabetes, obesity and metabolic syndrome; however, the specific mechanisms linking the gut microbiota with the host's energy homeostasis remain elusive. Probiotics are health-promoting bacteria that when consumed, alter the composition and/or metabolism of resident microbiota conferring health benefits. To assess the role of a specific probiotic treatment on microbiota-derived impacts on energy homeostasis in the context of development, Drosophila melanogaster larvae were orally administered the probiotic Lactobacillus fermentum NCIMB 5221 or its metabolic product, ferulic acid: a potent anti-inflammatory and anti-oxidant hydroxycinnamic acid. Read More

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http://dx.doi.org/10.1007/s12033-018-0119-yDOI Listing
January 2019

The Research Progress of Chalcone Isomerase (CHI) in Plants.

Mol Biotechnol 2019 Jan;61(1):32-52

School of Life Sciences, Beijing University of Chinese Medicine, Yangguang South Street, Fangshan District, Beijing, 102401, China.

Chalcone isomerase (CHI) is the second rate-limiting and the first reported enzyme involved in the biosynthetic pathway of flavonoids. It catalyzes the intramolecular cyclization reaction, converting the bicyclic chalcone into tricyclic (2S)-flavanone. In this paper, we obtained and analyzed 916 DNA sequences, 1310 mRNA sequences, and 2403 amino acid sequences of CHI registered in NCBI by Jan 2018. Read More

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http://link.springer.com/10.1007/s12033-018-0130-3
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http://dx.doi.org/10.1007/s12033-018-0130-3DOI Listing
January 2019
14 Reads

Characterization and Evaluation of Mango Germplasm Through Morphological, Biochemical, and Molecular Markers Focusing on Fruit Production: An Overview.

Mol Biotechnol 2018 Oct 12. Epub 2018 Oct 12.

Genomics Lab, Department of Plant Breeding & Genetics, Bahauddin Zakariya University, Multan, 60800, Pakistan.

Mango is also known as 'King of Fruits' and is one of the most popular fruit crops in tropical and sub-tropical regions. Characterization through different markers is an important need for mango germplasm conservation and its utilization for various breeding purposes. Description of existing germplasm is essential to detect anticipated traits or genes. Read More

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http://dx.doi.org/10.1007/s12033-018-0129-9DOI Listing
October 2018

Avian Bioreactor Systems: A Review.

Mol Biotechnol 2018 Dec;60(12):975-983

Department of Animal Sciences, The Ohio State University, Columbus, OH, 43210, USA.

Animal bioreactors are genetically modified animal systems that have the potential to reduce production cost, and improve production efficiency, of pharmaceutically relevant recombinant proteins. Several species including goats, cattle, rabbits, and avians have been genetically modified to secrete target proteins into milk, egg whites, blood, or other bodily fluids. There are several advantages associated with the use of avians as bioreactor systems. Read More

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http://dx.doi.org/10.1007/s12033-018-0128-xDOI Listing
December 2018
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Expression, Purification, and Characterization of Recombinant Human α-Antitrypsin Produced Using Silkworm-Baculovirus Expression System.

Mol Biotechnol 2018 Dec;60(12):924-934

Laboratory of Insect Genome Science, Kyushu University Graduate School of Bioresource and Bioenvironmental Science, Hakozaki 6-10-1, Higashi-ku, Fukuoka, 812-8581, Japan.

Human α-antitrypsin (AAT) is the most abundant serine proteinase inhibitor (serpin) in the human plasma. Commercially available AAT for the medications of deficiency of α-antitrypsin is mainly purified from human plasma. There is a high demand for a stable and low-cost supply of recombinant AAT (rAAT). Read More

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http://link.springer.com/10.1007/s12033-018-0127-y
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http://dx.doi.org/10.1007/s12033-018-0127-yDOI Listing
December 2018
7 Reads