9,109 results match your criteria Molecular biology of the cell[Journal]


G-protein-coupled formyl peptide receptors play a dual role in neutrophil chemotaxis and bacterial phagocytosis.

Mol Biol Cell 2018 Dec 12:mbcE18060358. Epub 2018 Dec 12.

Chemotaxis Signal Section, Laboratory of Immunogenetics, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Rockville, MD.

A dogma of innate immunity is that neutrophils use G-Protein-coupled receptors (GPCRs) for chemoattractant to chase bacteria through chemotaxis and then use phagocytic receptors coupled with tyrosine kinases to destroy opsonized bacteria via phagocytosis. Our current work showed that G-protein-coupled formyl peptide receptors (FPRs) directly mediate neutrophil phagocytosis. Mouse neutrophils lacking formyl peptide receptors (Fpr1/2) are defective in the phagocytosis of E. Read More

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http://dx.doi.org/10.1091/mbc.E18-06-0358DOI Listing
December 2018
1 Read

Actomyosin contractility modulates Wnt signaling through adherens junction stability.

Mol Biol Cell 2018 Dec 12:mbcE18060345. Epub 2018 Dec 12.

Department of Molecular Biology and Biochemistry, Centre for Cell Biology, Development and Disease, Simon Fraser University, Burnaby, V5A 1S6, Canada.

Actomyosin contractility can influence the canonical Wnt signaling pathway in processes like mesoderm differentiation and tissue stiffness during tumorigenesis. We identified that increased non-muscle myosin II activation and cellular contraction inhibited Wnt target gene transcription in developing Drosophila imaginal discs. Genetic interactions studies were used to show that this effect was due to myosin-induced accumulation of cortical F-actin resulting in clustering and accumulation of E-cadherin to the adherens junctions. Read More

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http://dx.doi.org/10.1091/mbc.E18-06-0345DOI Listing
December 2018

Single molecule dynamics of the P granule scaffold MEG-3 in the C. elegans zygote.

Mol Biol Cell 2018 Dec 12:mbcE18060402. Epub 2018 Dec 12.

Department of Biological Sciences, Dartmouth College, Hanover NH 03755, USA.

During the asymmetric division of the C. elegans zygote, germ (P) granules are disassembled in the anterior cytoplasm and stabilized/assembled in the posterior cytoplasm, leading to their inheritance by the germline daughter cell. P granule segregation depends on MEG-3 and MEG-4, which are enriched in P granules and in the posterior cytoplasm surrounding P granules. Read More

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http://dx.doi.org/10.1091/mbc.E18-06-0402DOI Listing
December 2018

Intersectin-1 interacts with the golgin, GCC88, to couple the actin network and Golgi architecture.

Mol Biol Cell 2018 Dec 12:mbcE18050313. Epub 2018 Dec 12.

The Department of Biochemistry and Molecular Biology and Bio21 Molecular Science and Biotechnology Institute, The University of Melbourne, Victoria 3010, Australia.

The maintenance of the Golgi ribbon relies on a dynamic balance between the actin and microtubule networks, however the pathways controlling actin networks remain poorly defined. Previously, we showed that the TGN membrane tether/golgin, GCC88, modulates the Golgi ribbon architecture (Gosavi et al., 2018). Read More

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http://dx.doi.org/10.1091/mbc.E18-05-0313DOI Listing
December 2018

Cdc42 negatively regulates endocytosis during apical membrane maintenance in live animals.

Mol Biol Cell 2018 Dec 12:mbcE18100615. Epub 2018 Dec 12.

Laboratory of Cellular and Molecular Biology, Center for Cancer Research, National Cancer Institute, National Institutes of Health, 37 Convent Dr. Rm 2050B, Bethesda, MD, 20892 USA.

Lumen establishment and maintenance are fundamental for tubular organs physiological functions. Most of the studies investigating the mechanisms regulating this process have been carried out in cell cultures or in smaller organisms, whereas little has been done in mammalian model systems in vivo. Here we used the salivary glands of live mice to examine the role of the small GTPase Cdc42 in the regulation of the homeostasis of the intercellular canaliculi, a specialized apical domain of the acinar cells, where protein and fluid secretion occur. Read More

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http://dx.doi.org/10.1091/mbc.E18-10-0615DOI Listing
December 2018
1 Read

Fer1l6 Is Essential for the Development of Vertebrate Muscle Tissue in Zebrafish.

Mol Biol Cell 2018 Dec 5:mbcE18060401. Epub 2018 Dec 5.

Department of Biochemistry and Biophysics, Oregon State University, Corvallis, OR 97331 USA.

The precise spatial and temporal expression of genes is essential for proper organismal development. Despite their importance however, many developmental genes have yet to be identified. We have determined that Fer1L6, a member of the ferlin family of genes, is a novel factor in zebrafish development. Read More

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https://www.molbiolcell.org/doi/10.1091/mbc.E18-06-0401
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http://dx.doi.org/10.1091/mbc.E18-06-0401DOI Listing
December 2018
7 Reads

Cyclin C directly stimulates Drp1 GTP affinity to mediate stress-induced mitochondrial hyper-fission.

Mol Biol Cell 2018 Dec 5:mbcE18070463. Epub 2018 Dec 5.

Graduate School of Biomedical Sciences, Department of Molecular Biology, Rowan University School of Osteopathic Medicine, Two Medical Center Drive, Stratford, NJ 08084 USA.

Mitochondria exist in an equilibrium between fragmented and fused that shifts heavily toward fission in response to cellular damage. Nuclear to cytoplasmic cyclin C relocalization is essential for dynamin-related protein 1 (Drp1)-dependent mitochondrial fission in response to oxidative stress. This study finds that cyclin C directly interacts with the Drp1 GTPase domain, increases its affinity to GTP and stimulates GTPase activity in vitro. Read More

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http://dx.doi.org/10.1091/mbc.E18-07-0463DOI Listing
December 2018
1 Read

SUMOylation of Periplakin is critical for efficient re-organization of Keratin filament network.

Mol Biol Cell 2018 Dec 5:mbcE18040244. Epub 2018 Dec 5.

Nups and SUMO Biology Group, Department of Biological Sciences, Indian Institute of Science Education and Research (IISER) Bhopal, Madhya Pradesh, India.

The architecture of the cytoskeleton and its remodeling are tightly regulated by dynamic reorganization of keratin-rich intermediate filaments. Plakin family proteins associate with the network of intermediate filaments (IFs) and affect its reorganization during migration, differentiation, and response to stress. The smallest plakin, periplakin (PPL), interacts specifically with intermediate filament proteins K8, K18, and vimentin via its C-terminal linker domain. Read More

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http://dx.doi.org/10.1091/mbc.E18-04-0244DOI Listing
December 2018

A rapid computational approach identifies SPICE1 as an Aurora kinase substrate.

Mol Biol Cell 2018 Nov 28:mbcE18080495. Epub 2018 Nov 28.

Wellcome Trust Centre for Cell Biology, School of Biological Sciences, University of Edinburgh, Edinburgh EH9 3JR, Scotland, UK.

Aurora kinases play a major role in mitosis by regulating diverse substrates. Defining their critical downstream targets is important to understand Aurora kinase function. Here we have developed an unbiased computational approach to identify new Aurora kinase substrates based on phosphorylation site clustering, protein localization, protein structure, and species conservation. Read More

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https://www.molbiolcell.org/doi/10.1091/mbc.E18-08-0495
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November 2018
3 Reads

Multi-layered regulation of TORC1-body formation in budding yeast.

Mol Biol Cell 2018 Nov 28:mbcE18050297. Epub 2018 Nov 28.

Department of Molecular and Cellular Biology, University of Arizona, Tucson AZ 85721-0206.

The Target of Rapamycin Kinase Complex 1 (TORC1) regulates cell growth and metabolism in eukaryotes. In Saccharomyces cerevisiae, TORC1 activity is known to be controlled by the conserved GTPases, Gtr1/2, and movement into and out of an inactive agglomerate/body. However, it is unclear if/how these regulatory steps are coupled. Read More

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https://www.molbiolcell.org/doi/10.1091/mbc.E18-05-0297
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http://dx.doi.org/10.1091/mbc.E18-05-0297DOI Listing
November 2018
4 Reads

Fatty-acid Binding Protein 5 modulates the SAR1 GTPase cycle and enhances budding of large COPII cargos.

Mol Biol Cell 2018 Nov 28:mbcE18090548. Epub 2018 Nov 28.

Department of Molecular and Cell Biology, Howard Hughes Medical Institute, University of California, Berkeley, Berkeley, United States.

COPII coated vesicles are the primary mediators of ER-to-Golgi trafficking. Sar1, one of the five core COPII components, is a highly conserved small GTPase, which, upon GTP binding, recruits the other COPII proteins to the ER membrane. It has been hypothesized that the changes in the kinetics of SAR1 GTPase may allow for the secretion of large cargos. Read More

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http://dx.doi.org/10.1091/mbc.E18-09-0548DOI Listing
November 2018

Cell biology of stem cells: studying stem cells at the level of cell biology and studying cell biology using stem cells.

Mol Biol Cell 2018 Nov;29(24):2912

Life Sciences Institute, Department of Cell and Developmental Biology, and Howard Hughes Medical Institute, University of Michigan, Ann Arbor, MI 48109.

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http://dx.doi.org/10.1091/mbc.E18-09-0596DOI Listing
November 2018

Paul Allen.

Authors:

Mol Biol Cell 2018 Nov;29(24):2911

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http://dx.doi.org/10.1091/mbc.E18-10-0673DOI Listing
November 2018

Stem cell models of human synapse development and degeneration.

Mol Biol Cell 2018 Nov;29(24):2913-2921

Department of Anatomy and Cell Biology, Brody School of Medicine, East Carolina University, Greenville, NC 27834.

Many brain disorders exhibit altered synapse formation in development or synapse loss with age. To understand the complexities of human synapse development and degeneration, scientists now engineer neurons and brain organoids from human-induced pluripotent stem cells (hIPSC). These hIPSC-derived brain models develop both excitatory and inhibitory synapses and functional synaptic activity. Read More

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http://dx.doi.org/10.1091/mbc.E18-04-0222DOI Listing
November 2018
1 Read

Combinatorial processing of bacterial and host-derived innate immune stimuli at the single-cell level.

Mol Biol Cell 2018 Nov 21:mbcE18070423. Epub 2018 Nov 21.

Department of Bioengineering, Stanford University, 443 Via Ortega, Stanford, CA 94305, USA.

During the course of a bacterial infection, cells are exposed simultaneously to a range of bacterial and host factors, which converge on the central transcription factor nuclear factor (NF)-κB. How do single cells integrate and process these converging stimuli? Here, we tackle the question of how cells process combinatorial signals by making quantitative single-cell measurements of the NF-κB response to combinations of bacterial lipopolysaccharide (LPS) and the stress cytokine Tumor Necrosis Factor (TNF). We found that cells encode the presence of both stimuli via the dynamics of NF-κB nuclear translocation in individual cells, suggesting the integration of NF-κB activity for these stimuli occurs at the molecular and pathway level. Read More

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http://dx.doi.org/10.1091/mbc.E18-07-0423DOI Listing
November 2018
2 Reads

ErbB4 tyrosine kinase inhibition impairs neuromuscular development in zebrafish embryos.

Mol Biol Cell 2018 Nov 21:mbcE18070460. Epub 2018 Nov 21.

From the Institute of Biomedicine, University of Turku, Turku, Finland.

Tyrosine kinase inhibitors are widely used in the clinic, but limited information is available about their toxicity in developing organisms. Here, we tested the effect of tyrosine kinase inhibitors targeting the ErbB receptors for their effects on developing zebrafish ( Danio rerio) embryos. Embryos treated with wide-spectrum pan-ErbB inhibitors or erbb4a-targeting antisense oligonucleotides demonstrated reduced locomotion, reduced diameter of skeletal muscle fibers, reduced expression of muscle-specific genes, as well as reduced motoneuron length. Read More

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http://dx.doi.org/10.1091/mbc.E18-07-0460DOI Listing
November 2018
2 Reads

Interphase cohesin regulation ensures mitotic fidelity after genome reduplication.

Mol Biol Cell 2018 Nov 21:mbcE17100582. Epub 2018 Nov 21.

Department of Cell Biology, Duke University Medical Center, Durham, NC 27710.

To ensure faithful genome propagation, mitotic cells alternate one round of chromosome duplication with one round of chromosome separation. Chromosome separation failure thus causes genome reduplication, which alters mitotic chromosome structure. Such structural alterations are well-documented to impair mitotic fidelity following aberrant genome re-duplication, including in diseased states. Read More

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http://dx.doi.org/10.1091/mbc.E17-10-0582DOI Listing
November 2018
3 Reads

Selective defects in gene expression control genome instability in yeast splicing mutants.

Mol Biol Cell 2018 Nov 21:mbcE18070439. Epub 2018 Nov 21.

Terry Fox Laboratory, British Columbia Cancer Agency, Vancouver Canada.

RNA processing mutants have been broadly implicated in genome stability but mechanistic links are often unclear. Two predominant models have emerged: one involving changes in gene expression that perturb other genome maintenance factors, and another in which genotoxic DNA:RNA hybrids, called R-loops, impair DNA replication. Here we characterize genome instability phenotypes in yeast splicing factor mutants and find that mitotic defects, and in some cases R-loop accumulation, are causes of genome instability. Read More

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http://dx.doi.org/10.1091/mbc.E18-07-0439DOI Listing
November 2018
8 Reads

Cellular tension encodes local Src-dependent differential β and β integrin mobility.

Mol Biol Cell 2018 Nov 21:mbcE18040253. Epub 2018 Nov 21.

Institut Albert Bonniot, Université Joseph Fourier; INSERM U823; CNRS ERL 5284, Grenoble Alpessite Santé, BP170, F38042 Grenoble cedex 09, France.

Integrins are transmembrane receptors that have a pivotal role in mechanotransduction processes by connecting the extracellular matrix to the cytoskeleton. Although it is well established that integrin activation/inhibition cycles are due to highly dynamic interactions, whether integrin mobility depends on local tension and cytoskeletal organization remains surprisingly unclear. Using an original approach combining micropatterning on glass substrates to induce standardized local mechanical constraints within a single cell with temporal image correlation spectroscopy (tICS), we measured the mechanosensitive response of integrin mobility at the whole cell level and in adhesion sites under different mechanical constraints. Read More

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http://dx.doi.org/10.1091/mbc.E18-04-0253DOI Listing
November 2018
4 Reads

An endogenous chemorepellent directs cell movement by inhibiting pseudopods at one side of cells.

Mol Biol Cell 2018 Nov 21:mbcE18090562. Epub 2018 Nov 21.

Department of Biology, Texas A&M University, College Station, TX 77843-3474 USA.

Eukaryotic chemoattraction signal transduction pathways, such as those used by Dictyostelium discoideum to move towards cAMP, use a G protein-coupled receptor to activate multiple conserved pathways such as PI3 kinase/Akt/PKB to induce actin polymerization and pseudopod formation at the front of a cell, and PTEN to localize myosin II to the rear of a cell. Relatively little is known about chemorepulsion. We previously found that AprA is a chemorepellent protein secreted by Dictyostelium cells. Read More

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http://dx.doi.org/10.1091/mbc.E18-09-0562DOI Listing
November 2018
7 Reads
4.470 Impact Factor

Effects of cardiomyopathy-linked mutations k15n and r21h in tropomyosin on thin filament regulation and pointed-end dynamics.

Mol Biol Cell 2018 Nov 21:mbcE18060406. Epub 2018 Nov 21.

Voiland School of Chemical Engineering and Bioengineering, Washington State University, Pullman, WA.

Missense mutations K15N and R21H in striated muscle tropomyosin are linked to dilated cardiomyopathy (DCM) and hypertrophic cardiomyopathy (HCM), respectively. Tropomyosin, together with the troponin complex, regulates muscle contraction and, along with tropomodulin and leiomodin, controls the uniform thin filament lengths crucial for normal sarcomere structure and function. We used Förster resonance energy transfer to study effects of the tropomyosin mutations on the structure and kinetics of the cardiac troponin core domain associated with the Ca-dependent regulation of cardiac thin filaments. Read More

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http://dx.doi.org/10.1091/mbc.E18-06-0406DOI Listing
November 2018
2 Reads

Phosphatidylinositol 3,5-Bisphosphate Regulates the Transition between trans-SNARE Complex Formation and Vacuole Membrane Fusion.

Mol Biol Cell 2018 Nov 14:mbcE18080505. Epub 2018 Nov 14.

Department of Biochemistry, University of Illinois at Urbana-Champaign, Urbana, IL 61801.

Phosphoinositides (PIs) regulate myriad cellular functions including membrane fusion, as exemplified by the yeast vacuole, which uses various PIs at different stages of fusion. In light of this, the effect of phosphatidylinositol 3,5-bisphosphate [PI(3,5)P] on vacuole fusion remains unknown. PI(3,5)P is made by the PI3P 5-kinase Fab1 and has been characterized as a regulator of vacuole fission during hyperosmotic shock where it interacts with the TRP Ca channel Yvc1. Read More

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https://www.molbiolcell.org/doi/10.1091/mbc.E18-08-0505
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http://dx.doi.org/10.1091/mbc.E18-08-0505DOI Listing
November 2018
2 Reads

ADP-ribosylation factor-like 4A interacts with Robo1 to promote cell migration by regulating Cdc42 activation.

Mol Biol Cell 2018 Nov 14:mbcE18010001. Epub 2018 Nov 14.

Institute of Molecular Medicine, College of Medicine, National Taiwan University, Taipei 10002, Taiwan.

Cell migration is a highly regulated event that is initiated by cell membrane protrusion and actin reorganization. Robo1, a single-pass transmembrane receptor, is crucial for neuronal guidance and cell migration. ADP-ribosylation factor (Arf)-like 4A (Arl4A), an Arf small GTPase, functions in cell morphology, cell migration, and actin cytoskeleton remodeling; however, the molecular mechanisms of Arl4A in cell migration are unclear. Read More

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https://www.molbiolcell.org/doi/10.1091/mbc.E18-01-0001
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http://dx.doi.org/10.1091/mbc.E18-01-0001DOI Listing
November 2018
3 Reads

The roles of a flagellar HSP40 ensuring rhythmic beating.

Mol Biol Cell 2018 Nov 14:mbcE18010047. Epub 2018 Nov 14.

Department of Biological Sciences, Marquette University, 530 N. 15th St. Milwaukee, WI 53233, USA.

HSP40s are regarded as co-chaperones perpetually shuttling client polypeptides to HSP70s for refolding. However, many HSP40s central for disparate processes diverge from this paradigm. To elucidate the noncanonical mechanisms, we investigated the HSP40 in the radial spoke (RS) complex in flagella. Read More

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https://www.molbiolcell.org/doi/10.1091/mbc.E18-01-0047
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http://dx.doi.org/10.1091/mbc.E18-01-0047DOI Listing
November 2018
3 Reads

The J-domain co-chaperone Rsp1 interacts with Mto1 to organize non-centrosomal microtubule assembly.

Mol Biol Cell 2018 Nov 14:mbcE18050279. Epub 2018 Nov 14.

Division of Molecular & Cell Biophysics, Hefei National Science Center for Physical Sciences, University of Science and Technology of China, Hefei, Anhui, China.

Microtubule biogenesis initiates at various intracellular sites, including the centrosome, the Golgi apparatus, the nuclear envelope, and pre-existing microtubules. Similarly, in the fission yeast Schizosaccharomyces pombe, interphase microtubules are nucleated at the spindle pole body (SPB), the nuclear envelope, and pre-existing microtubules, depending on Mto1 activity. Despite the essential role of Mto1 in promoting microtubule nucleation, it has remained elusive as how distribution of Mto1 in different sites is regulated. Read More

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https://www.molbiolcell.org/doi/10.1091/mbc.E18-05-0279
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http://dx.doi.org/10.1091/mbc.E18-05-0279DOI Listing
November 2018
6 Reads

Brush border protocadherin CDHR2 promotes the elongation and maximized packing of microvilli in vivo.

Mol Biol Cell 2018 Nov 7:mbcE18090558. Epub 2018 Nov 7.

Department of Cell and Developmental Biology, Vanderbilt University School of Medicine, Nashville, TN.

Transporting epithelial cells optimize their morphology for solute uptake by building an apical specialization: a dense array of microvilli that serves to increase membrane surface area. In the intestinal tract, individual cells build thousands of microvilli, which pack tightly to form the brush border. Recent studies implicate adhesion molecule CDHR2 in the regulation of microvillar packing via the formation of adhesion complexes between the tips of adjacent protrusions. Read More

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https://www.molbiolcell.org/doi/10.1091/mbc.E18-09-0558
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November 2018
12 Reads
4.470 Impact Factor

The ARP2/3 complex prevents excessive formin activity during cytokinesis.

Mol Biol Cell 2018 Nov 7:mbcE18070471. Epub 2018 Nov 7.

Instituto de Investigação e Inovação em Saúde (i3S), Universidade do Porto, 4200-135 Porto, Portugal.

Cytokinesis completes cell division by constriction of an acto-myosin contractile ring that separates the two daughter cells. Here, we use the early C. elegans embryo to explore how the actin filament network in the ring and the surrounding cortex is regulated by the single cytokinesis formin CYK-1 and the ARP2/3 complex, which nucleate non-branched and branched filaments, respectively. Read More

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https://www.molbiolcell.org/doi/10.1091/mbc.E18-07-0471
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http://dx.doi.org/10.1091/mbc.E18-07-0471DOI Listing
November 2018
6 Reads

Different SUMO paralogs determine the fate of WT and mutant CFTRs: biogenesis vs. degradation.

Mol Biol Cell 2018 Nov 7:mbcE18040252. Epub 2018 Nov 7.

Departments of Pediatrics and Cell Biology, University of Pittsburgh School of Medicine, Rangos Research Center, 4401 Penn Avenue, Pittsburgh, PA 15224.

A pathway for CFTR degradation is initiated by Hsp27 which cooperates with Ubc9 and binds to the common F508del mutant to modify it with SUMO-2/3. These SUMO paralogs form poly-chains, which are recognized by the ubiquitin ligase, RNF4, for proteosomal degradation. Here, protein array analysis identified the SUMO E3, PIAS4, which increased WT and F508del CFTR biogenesis in CFBE airway cells. Read More

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https://www.molbiolcell.org/doi/10.1091/mbc.E18-04-0252
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http://dx.doi.org/10.1091/mbc.E18-04-0252DOI Listing
November 2018
12 Reads

In vitro reconstitution of DNA replication initiated by genetic recombination: A T4 bacteriophage model for a type of DNA synthesis important for all cells.

Mol Biol Cell 2018 Nov 7:mbcE18060386. Epub 2018 Nov 7.

Department of Biochemistry and Biophysics, University of California, San Francisco, San Francisco, California 94158-2517.

Using a mixture of ten purified DNA replication and DNA recombination proteins encoded by the bacteriophage T4 genome, plus two homologous DNA molecules, we have reconstituted the genetic recombination-initiated pathway that initiates DNA replication forks at late times of T4 bacteriophage infection. Inside the cell, this recombination-dependent replication (RDR) is required to produce the long concatemeric T4 DNA molecules that serve as substrates for packaging the shorter, genome-sized viral DNA into phage heads. The five T4 proteins that catalyze DNA synthesis on the leading strand plus the proteins required for lagging strand DNA synthesis are essential for the reaction, as are a special mediator protein (gp59) and a Rad51/RecA analog (the T4 UvsX strand-exchange protein). Read More

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https://www.molbiolcell.org/doi/10.1091/mbc.E18-06-0386
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http://dx.doi.org/10.1091/mbc.E18-06-0386DOI Listing
November 2018
6 Reads

Robust and stable transcriptional repression in Giardia using CRISPRi.

Mol Biol Cell 2018 Oct 31:mbcE18090605. Epub 2018 Oct 31.

Department of Microbiology and Molecular Genetics, One Shields Avenue, UC Davis, Davis, CA 95616.

Giardia lamblia is a binucleate protistan parasite causing significant diarrheal disease worldwide. An inability to target Cas9 to both nuclei, combined with the lack of non-homologous end joining and markers for positive selection, has stalled the adaptation of CRISPR/Cas9-mediated genetic tools for this widespread parasite. CRISPR interference (CRISPRi) is a modification of the CRISPR/Cas9 system that directs catalytically inactive Cas9 (dCas9) to target loci for stable transcriptional repression. Read More

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https://www.molbiolcell.org/doi/10.1091/mbc.E18-09-0605
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http://dx.doi.org/10.1091/mbc.E18-09-0605DOI Listing
October 2018
6 Reads

Nuclear envelope expansion in budding yeast is independent of cell growth and does not determine nuclear volume.

Mol Biol Cell 2018 Oct 31:mbcE18040204. Epub 2018 Oct 31.

The Laboratory of Molecular and Cellular Biology, NIDDK, NIH, Bethesda, MD 20892.

Most cells exhibit a constant ratio between nuclear and cell volume. The mechanism dictating this constant ratio and the nuclear component(s) that scale with cell size are not known. To address this, we examined the consequences to the size and shape of the budding yeast nucleus when cell expansion is inhibited by down-regulating components of the secretory pathway. Read More

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http://dx.doi.org/10.1091/mbc.E18-04-0204DOI Listing
October 2018

Comprehensive analysis of formin localization in Xenopus epithelial cells.

Mol Biol Cell 2018 Oct 31:mbcE18020133. Epub 2018 Oct 31.

Department of Molecular, Cellular, and Developmental Biology, University of Michigan, Ann Arbor, MI 48109, United States.

Reorganization of the actin cytoskeleton is crucial for cellular processes including cytokinesis and cell-cell junction remodeling. Formins are conserved processive actin polymerizing machines that regulate actin dynamics by nucleating, elongating, and bundling linear actin filaments. Because the formin family is large, with at least 15 members in vertebrates, there have not been any comprehensive studies examining formin localization and function within a common cell type. Read More

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http://dx.doi.org/10.1091/mbc.E18-02-0133DOI Listing
October 2018

Ventricular-subventricular zone fractones are speckled basement membranes that function as a neural stem cell niche.

Mol Biol Cell 2018 Oct 31:mbcE18050286. Epub 2018 Oct 31.

Laboratory of Extracellular Matrix Biochemistry, Institute for Protein Research, Osaka University, Suita, Osaka 565-0871, Japan.

Neural stem cells (NSCs) are retained in the adult ventricular-subventricular zone (V-SVZ), a specialized neurogenic niche with a unique cellular architecture. It currently remains unclear whether or how NSCs utilize basement membranes (BMs) in the niche. Here, we examined the molecular compositions and functions of BMs in the adult mouse V-SVZ. Read More

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http://dx.doi.org/10.1091/mbc.E18-05-0286DOI Listing
October 2018
5 Reads

Environmental stresses induce karyotypic instability in colorectal cancer cells.

Mol Biol Cell 2018 Oct 31:mbcE18100626. Epub 2018 Oct 31.

Institute of Medical Biology, Agency for Science, Technology and Research (A*STAR), Singapore, Singapore.

Understanding how cells acquire genetic mutations is a fundamental biological question with implications for many different areas of biomedical research, ranging from tumor evolution to drug resistance. While karyotypic heterogeneity is a hallmark of cancer cells, few mutations causing chromosome instability have been identified in cancer genomes, suggesting a non-genetic origin of this phenomenon. Accordingly, we found that in vitro exposure of karyotypically stable human colorectal cancer cell lines to environmental stress conditions triggered a wide variety of chromosomal changes and karyotypic heterogeneity. Read More

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http://dx.doi.org/10.1091/mbc.E18-10-0626DOI Listing
October 2018
1 Read

Reductions in ATPase activity, actin sliding velocity and myofibril stability yield muscle dysfunction in Drosophila models of myosin-based Freeman Sheldon syndrome.

Mol Biol Cell 2018 Oct 31:mbcE18080526. Epub 2018 Oct 31.

Department of Biology, Molecular Biology Institute and Heart Institute, San Diego State University, San Diego, CA 92182-4614, USA.

Using Drosophila melanogaster we created the first animal models for myosin-based Freeman Sheldon Syndrome, a dominant form of distal arthrogryposis defined by congenital facial and distal skeletal muscle contractures. Electron microscopy of homozygous mutant indirect flight muscles showed normal (Y583S) or altered (T178I, R672C) myofibril assembly, followed by progressive disruption of the myofilament lattice. In contrast, all alleles permitted normal myofibril assembly in the heterozygous state, but caused myofibrillar disruption during aging. Read More

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https://www.molbiolcell.org/doi/10.1091/mbc.E18-08-0526
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October 2018
2 Reads

Geometric instability catalyzes mitochondrial fission.

Mol Biol Cell 2018 Oct 31:mbcE18010018. Epub 2018 Oct 31.

Department of Mechanical Engineering, University of Houston, Houston, TX, USA.

The mitochondrial membrane undergoes extreme remodeling during fission. While a few membrane-squeezing proteins are recognized as the key drivers of fission, there is a growing body of evidence that strongly suggests that conical lipids play a critical role in regulating mitochondrial morphology and fission. However, the mechanisms by which proteins and lipids cooperate to execute fission have not been quantitatively investigated. Read More

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https://www.researchgate.net/publication/262020327_A_dimeric
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https://www.molbiolcell.org/doi/10.1091/mbc.E18-01-0018
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http://dx.doi.org/10.1091/mbc.E18-01-0018DOI Listing
October 2018
5 Reads

ACVR1 FOP mutation alters mechanosensing and tissue stiffness during heterotopic ossification.

Mol Biol Cell 2018 Oct 31:mbcE18050311. Epub 2018 Oct 31.

Departments of Orthopaedic Surgery, University of Pennsylvania, Philadelphia, PA.

An activating BMP type I receptor ACVR1 (ACVR1) mutation enhances BMP pathway signaling and causes the rare genetic disorder of heterotopic (extra-skeletal) bone formation fibrodysplasia ossificans progressiva (FOP). Heterotopic ossification frequently occurs following injury as cells aberrantly differentiate during tissue repair. Biomechanical signals from the tissue microenvironment and cellular responses to these physical cues, such as stiffness/rigidity, are important determinants of cell differentiation and are modulated by BMP signaling. Read More

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https://www.molbiolcell.org/doi/10.1091/mbc.E18-05-0311
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http://dx.doi.org/10.1091/mbc.E18-05-0311DOI Listing
October 2018
3 Reads

The entrance: how life experience shaped my passion for diversity and inclusion.

Authors:
Ahna R Skop

Mol Biol Cell 2018 Nov;29(22):2608-2610

Laboratory of Genetics, University of Wisconsin-Madison, Madison, WI 53706.

I am deeply honored to receive the American Society for Cell Biology (ASCB) Prize for Excellence in Inclusivity made possible through a grant from the Howard Hughes Medical Institute. This generous award of $5000 will go toward travel and registration support for underrepresented students from the University of Wisconsin-Madison to attend the ASCB and SACNAS (Society for Advancement of Chicanos/Hispanics and Native Americans in Science) conferences. In this essay, I have woven together a few stories on how my life experiences have shaped my passion for diversity and inclusion in STEM. Read More

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https://www.molbiolcell.org/doi/10.1091/mbc.E18-07-0431
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http://dx.doi.org/10.1091/mbc.E18-07-0431DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6249843PMC
November 2018
1 Read

Who mentors whom?

Authors:
Eva Nogales

Mol Biol Cell 2018 Nov;29(22):2606-2607

Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA 94720; Howard Hughes Medical Institute, Berkeley, CA 94720; Molecular Biophysics and Integrated Bio-Imaging Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720.

This brief write-up describes my personal take on the mentor-mentee relationship as I have lived it while a professor at Berkeley. Ultimately, it is just a shout out to those who passed through my lab and shared with me their early scientific experiences. Read More

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https://www.molbiolcell.org/doi/10.1091/mbc.E18-07-0451
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http://dx.doi.org/10.1091/mbc.E18-07-0451DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6249833PMC
November 2018
4 Reads

Sex and death: from cell fate specification to dynamic control of X-chromosome structure and gene expression.

Authors:
Barbara J Meyer

Mol Biol Cell 2018 Nov;29(22):2616-2621

Howard Hughes Medical Institute and Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA 94720-3204.

Determining sex is a binary developmental decision that most metazoans must make. Like many organisms, Caenorhabditis elegans specifies sex (XO male or XX hermaphrodite) by tallying X-chromosome number. We dissected this precise counting mechanism to determine how tiny differences in concentrations of signals are translated into dramatically different developmental fates. Read More

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http://dx.doi.org/10.1091/mbc.E18-06-0397DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6249838PMC
November 2018

One step at a time.

Mol Biol Cell 2018 Nov;29(22):2614-2615

Department of Genetics, University of Texas MD Anderson Cancer Center, Houston, TX 77030.

The most challenging part of growing up a minority is identifying opportunities to follow your passion. My story is a bit convoluted, but a passion for science and learning coupled with motivation led to a stellar career that now includes the E.E. Read More

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http://dx.doi.org/10.1091/mbc.E18-06-0396DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6249840PMC
November 2018

CBE-Life Sciences Education: the story of a "great journal scientists might be caught reading".

Authors:
Erin L Dolan

Mol Biol Cell 2018 Nov;29(22):2611-2613

Department of Biochemistry and Molecular Biology, University of Georgia, Athens, GA 30602.

How did a moderately sized scientific society create what many consider to be the leading journal in biology education? As Editor-in-Chief of the education journal of the American Society for Cell Biology (ASCB), CBE-Life Sciences Education ( LSE) and recipient of the 2018 Bruce Alberts Award for Excellence in Science Education, I tell the story of the establishment, growth, and impact of ASCB's "other journal." Read More

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http://dx.doi.org/10.1091/mbc.E18-06-0410DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6249828PMC
November 2018

Let curiosity lead you.

Authors:
Elizabeth H Chen

Mol Biol Cell 2018 Nov;29(22):2603-2605

Department of Molecular Biology, Department of Cell Biology, and Hamon Center for Regenerative Science and Medicine, University of Texas Southwestern Medical Center, Dallas, TX 75390.

It is an incredible honor to receive the Woman in Cell Biology Mid-Career Award for Excellence in Research. My lab works on cell-cell fusion, an indispensable process in the conception, development, and physiology of multicellular organisms. In this essay, I reflect on my curiosity-led journey, which uncovered some unexpected mechanisms underlying cell-cell fusion. Read More

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http://dx.doi.org/10.1091/mbc.E18-08-0499DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6249829PMC
November 2018

Mating in wild yeast: delayed interest in sex after spore germination.

Mol Biol Cell 2018 Oct 24:mbcE18080528. Epub 2018 Oct 24.

Department of Pharmacology and Cancer Biology, Duke University Medical Center, Durham, NC 27710.

Studies of lab strains of Saccharomyces cerevisiae have uncovered signaling pathways involved in mating, including information processing strategies to optimize decisions to mate or to bud. However, lab strains are heterothallic (unable to self-mate) while wild yeast are homothallic. And while mating of lab strains is studied using cycling haploid cells, mating of wild yeast is thought to involve germinating spores. Read More

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https://www.molbiolcell.org/doi/10.1091/mbc.E18-08-0528
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http://dx.doi.org/10.1091/mbc.E18-08-0528DOI Listing
October 2018
2 Reads

Nup2 performs diverse interphase functions in Aspergillus nidulans.

Mol Biol Cell 2018 Dec 24;29(26):3144-3154. Epub 2018 Oct 24.

Department of Molecular Genetics, The Ohio State University, Columbus, OH 43210.

The nuclear pore complex (NPC) protein Nup2 plays interphase nuclear transport roles and in Aspergillus nidulans also functions to bridge NPCs at mitotic chromatin for their faithful coinheritance to daughter G1 nuclei. In this study, we further investigate the interphase functions of Nup2 in A. nidulans. Read More

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https://www.molbiolcell.org/doi/10.1091/mbc.E18-04-0223
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http://dx.doi.org/10.1091/mbc.E18-04-0223DOI Listing
December 2018
7 Reads

Atg1 mediated autophagy suppresses tissue degeneration in pink1/parkin mutants by promoting mitochondrial fission in Drosophila.

Mol Biol Cell 2018 Oct 24:mbcE18040243. Epub 2018 Oct 24.

Department of Neurology, Department of Molecular and Medical Pharmacology, UCLA David Geffen School of Medicine, University of California, Los Angeles, CA 90095, United States.

Mitochondria dysfunction is considered as a hallmark of multiple neuro-degenerative diseases, including Parkinson's disease (PD). PD familial genes, Pink1 and parkin function in a conserved pathway that regulates mitochondrial function including dynamics (fusion and fission). Mammalian cell culture studies suggested that pink1/ parkin pathway promotes mitophagy (mitochondrial autophagy). Read More

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http://dx.doi.org/10.1091/mbc.E18-04-0243DOI Listing
October 2018

The hnRNP raly regulates PRMT1 expression and interacts with the ALS-linked protein FUS: implication for reciprocal cellular localization.

Mol Biol Cell 2018 Oct 24:mbcE18020108. Epub 2018 Oct 24.

Laboratory of Molecular and Cellular Neurobiology, CIBIO-Centre for Integrative Biology, University of Trento.

RALY is a member of the heterogeneous nuclear ribonucleoprotein family whose transcriptome and interactome have been recently characterized. RALY binds poly-U rich elements within several RNAs and regulates the expression as well as the stability of specific transcripts. Here we show that RALY binds PRMT1 mRNA and regulates its expression. Read More

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http://dx.doi.org/10.1091/mbc.E18-02-0108DOI Listing
October 2018
7 Reads

Methionine coordinates a hierarchically organized anabolic program enabling proliferation.

Mol Biol Cell 2018 Oct 24:mbcE18080515. Epub 2018 Oct 24.

Institute for Stem Cell biology and Regenerative Medicine (inStem), NCBS-TIFR campus, GKVK Post, Bellary Road, Bangalore 560065.

Methionine availability during overall amino acid limitation metabolically reprograms cells to support proliferation, the underlying basis for which remains unclear. Here, we construct the organization of this methionine mediated anabolic program, using yeast. Combining comparative transcriptome analysis, biochemical and metabolic flux based approaches, we discover that methionine rewires overall metabolic outputs by increasing the activity of a key regulatory node. Read More

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https://www.biorxiv.org/content/biorxiv/early/2018/06/28/249
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https://www.molbiolcell.org/doi/10.1091/mbc.E18-08-0515
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http://dx.doi.org/10.1091/mbc.E18-08-0515DOI Listing
October 2018
2 Reads
4.470 Impact Factor

Ccdc61 controls centrosomal localization of Cep170 and is required for spindle assembly and symmetry.

Mol Biol Cell 2018 Oct 24:mbcE18020115. Epub 2018 Oct 24.

Cell Cycle Control and Carcinogenesis, F045, German Cancer Research Center, DKFZ, 69120 Heidelberg, Germany.

Microtubule nucleation was uncovered as a key principle of spindle assembly. However, the mechanistic details about microtubule nucleation and the organization of spindle formation and symmetry are currently being revealed. Here we describe the function of Coiled-coil domain containing 61 (Ccdc61), a so far uncharacterized centrosomal protein, in spindle assembly and symmetry. Read More

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https://www.molbiolcell.org/doi/10.1091/mbc.E18-02-0115
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http://dx.doi.org/10.1091/mbc.E18-02-0115DOI Listing
October 2018
5 Reads

Genetic and epigenetic determinants establish a continuum of Hsf1 occupancy and activity across the yeast genome.

Mol Biol Cell 2018 Dec 17;29(26):3168-3182. Epub 2018 Oct 17.

Department of Biochemistry and Molecular Biology, Louisiana State University Health Sciences Center, Shreveport, LA 71130.

Heat shock factor 1 is the master transcriptional regulator of molecular chaperones and binds to the same cis-acting heat shock element (HSE) across the eukaryotic lineage. In budding yeast, Hsf1 drives the transcription of ∼20 genes essential to maintain proteostasis under basal conditions, yet its specific targets and extent of inducible binding during heat shock remain unclear. Here we combine Hsf1 chromatin immunoprecipitation sequencing (seq), nascent RNA-seq, and Hsf1 nuclear depletion to quantify Hsf1 binding and transcription across the yeast genome. Read More

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http://dx.doi.org/10.1091/mbc.E18-06-0353DOI Listing
December 2018