9,886 results match your criteria Molecular and cellular endocrinology[Journal]


Effects of hypothyroidism on the mesenteric and omental adipose tissue in rats.

Mol Cell Endocrinol 2019 Apr 17. Epub 2019 Apr 17.

Instituto de Medicina y Biología Experimental de Cuyo (IMBECU), CONICET, CCT-Mendoza, Argentina. Electronic address:

To characterize the influence of hypothyroidism on the endocrine activity of mesenteric and omental adipose tissue (MOAT) and the peripheral regulation of energy balance (EB) in rats, we analyzed food intake (FI); basal metabolic rate (BMR); locomotor activity; body weight (BW); serum hormone concentrations and the expression of their receptors in MOAT. We evaluated the morphology and differentiation of adipocytes. Hypothyroidism decreased FI, BMR and BW. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S03037207193011
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http://dx.doi.org/10.1016/j.mce.2019.04.011DOI Listing
April 2019
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Differential Expression of HMGA1 and HMGA2 in pituitary neuroendocrine tumors.

Mol Cell Endocrinol 2019 Apr 15. Epub 2019 Apr 15.

Laboratory of Experimental Endocrinology - LEEx, Institute of Biomedical Sciences, Federal University of Rio de Janeiro, Brazil; Graduate Program in Endocrinology, Faculty of Medicine, Federal University of Rio de Janeiro, Brazil; Graduate Program in Pharmacology and Medicinal Chemistry, Institute of Biomedical Sciences, Federal University of Rio de Janeiro, Brazil. Electronic address:

Defining biomarkers for invasive pituitary neuroendocrine tumors (PitNETs) is highly desirable. The high mobility group A (HMGA) proteins are among the most widely expressed cancer-associated proteins. Indeed, their overexpression is a frequent feature of human malignancies, including PitNETs. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S03037207193011
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http://dx.doi.org/10.1016/j.mce.2019.04.010DOI Listing
April 2019
1 Read

Editorial: Biology and clinical relevance of Hydroxysteroid (17beta) dehydrogenase enzymes.

Authors:
Matti Poutanen

Mol Cell Endocrinol 2019 Apr 13. Epub 2019 Apr 13.

Institute of Biomedicine, Research Centre for Integrative Physiology and Pharmacology, University of Turku, Finland; Institute of Medicine, The Sahlgrenska Academy, University of Gothenburg, Sweden.

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http://dx.doi.org/10.1016/j.mce.2019.04.008DOI Listing
April 2019
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Long non-coding RNA LINC01207 silencing suppresses AGR2 expression to facilitate autophagy and apoptosis of pancreatic cancer cells by sponging miR-143-5p.

Mol Cell Endocrinol 2019 Apr 13. Epub 2019 Apr 13.

Department of Pathology, Shengjing Hospital of China Medical University, Shenyang, 110004, PR China. Electronic address:

Pancreatic cancer is a serious malignancy accompanied by a well-documented poor prognosis. Accumulating studies have indicated the crucial roles played by long non-coding RNAs (lncRNAs) in proliferation, apoptosis and invasion of cancer cells. The aim of the current study was to investigate the role of lncRNA LINC01207 in autophagy and apoptosis of pancreatic cancer cells and its regulatory mechanism interacting with miR-143-5p. Read More

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http://dx.doi.org/10.1016/j.mce.2019.04.004DOI Listing
April 2019
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Nuclear estrogen receptor activation by insulin-like growth factor-1 in Neuro-2A neuroblastoma cells requires endogenous estrogen synthesis and is mediated by mutually repressive MAPK and PI3K cascades.

Mol Cell Endocrinol 2019 Apr 12. Epub 2019 Apr 12.

Tulane Brain Institute, Tulane University, 200 Flower Hall, New Orleans, LA, 70118, USA; Neuroscience Program, Tulane University, 200 Flower Hall, New Orleans, LA, 70118, USA; Department of Psychology, Tulane Universit, 2007 Percival Stern Hall, New Orleans, LA, 70118, USA.

Non-canonical mechanisms of estrogen receptor activation may continue to support women's cognitive health long after cessation of ovarian function. These mechanisms of estrogen receptor activation may include ligand-dependent actions via locally synthesized neuroestrogens and ligand-independent actions via growth factor-dependent activation of intracellular kinase cascades. We tested the hypothesis that ligand-dependent and ligand-independent mechanisms interact to activate nuclear estrogen receptors in the Neuro-2A neuroblastoma cell line in the absence of exogenous estrogens. Read More

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http://dx.doi.org/10.1016/j.mce.2019.04.007DOI Listing

Endovascular trophoblast expresses CD59 to evade complement-dependent cytotoxicity.

Mol Cell Endocrinol 2019 Apr 11. Epub 2019 Apr 11.

Department of Gynecology and Obstetrics, Kyoto University Graduate School of Medicine, Kyoto, 606-8507, Japan.

In the human placenta, extravillous trophoblasts (EVTs) invade maternal decidual tissues (interstitial trophoblasts) and maternal spiral arteries (endovascular trophoblasts). Although endovascular trophoblasts are directly exposed to maternal blood containing complement components, they are not eliminated by complement-dependent cytotoxicity (CDC). In this study, we investigated the expression and possible function of CD59, one of the membrane-bound complement regulators, in EVTs. Read More

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http://dx.doi.org/10.1016/j.mce.2019.04.006DOI Listing
April 2019
2 Reads

Disruption of ESR1 alters the expression of genes regulating hepatic lipid and carbohydrate metabolism in male rats.

Mol Cell Endocrinol 2019 Apr 8. Epub 2019 Apr 8.

Department of Pathology and Laboratory Medicine, University of Kansas Medical Center, Kansas City, KS, 66160, USA; Institute for Reproduction and Perinatal Research, University of Kansas Medical Center, Kansas City, KS, 66160, USA. Electronic address:

The liver helps maintain energy homeostasis by synthesizing and storing glucose and lipids. Gonadal steroids, particularly estrogens, play an important role in regulating metabolism. As estrogens are considered female hormones, metabolic disorders related to the disruption of estrogen signaling have mostly been studied in females. Read More

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http://dx.doi.org/10.1016/j.mce.2019.04.005DOI Listing
April 2019
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Elimination of adrenocortical apolipoprotein E production does not impact glucocorticoid output in wild-type mice.

Mol Cell Endocrinol 2019 Apr 3. Epub 2019 Apr 3.

Division of BioTherapeutics, Leiden Academic Centre for Drug Research, 2333CC, Leiden, the Netherlands.

Apolipoprotein E (APOE) deficient mice exhibit unexplained hypercorticosteronemia. Given that APOE is also produced locally within the adrenals, we evaluated the effect of adrenal-specific APOE deficiency on the glucocorticoid function. Hereto, one adrenal containing or lacking APOE was transplanted into adrenalectomized wild-type mice. Read More

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http://dx.doi.org/10.1016/j.mce.2019.04.001DOI Listing
April 2019
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Non-canonical cyclic AMP SMAD1/5/8 signalling in human granulosa cells.

Mol Cell Endocrinol 2019 Apr 3. Epub 2019 Apr 3.

School of Women's and Children's Health, Fertility and Research Centre, University of New South Wales Sydney, NSW, 2052, Australia.

Development of mammalian ovarian follicles is promoted by the combined action of endocrine cues and paracrine factors. Follicle stimulating hormone (FSH), through the action of cAMP drives follicular growth and development. The oocyte secretes powerful growth factors such as bone morphogenetic protein 15 (BMP15) to regulate granulosa cell proliferation, metabolism, steroidogenesis and differentiation through the activation of SMAD1/5/8. Read More

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http://dx.doi.org/10.1016/j.mce.2019.04.003DOI Listing

Estrogen interacts with glucocorticoids in the regulation of lipocalin 2 expression in human adipose tissue. Reciprocal roles of estrogen receptor α and β in insulin resistance?

Mol Cell Endocrinol 2019 Apr 3. Epub 2019 Apr 3.

Department of Medical Sciences, Clinical Diabetes and Metabolism, Uppsala University, Uppsala, Sweden. Electronic address:

The adipokine lipocalin 2 (LCN2) is linked to insulin resistance. Its expression in human adipose tissue (AT) can be regulated in a sex-specific manner by a synthetic glucocorticoid, dexamethasone, suggesting an underlying role of sex steroids. We show that 17-β-estradiol (E2) dose-dependently increased LCN2 gene expression in subcutaneous AT from postmenopausal women. Read More

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http://dx.doi.org/10.1016/j.mce.2019.04.002DOI Listing
April 2019
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Cryo-EM structures of GPCRs coupled to G, G and G.

Mol Cell Endocrinol 2019 May 28;488:1-13. Epub 2019 Mar 28.

MRC Laboratory of Molecular Biology, Francis Crick Avenue, Cambridge, CB2 0QH, UK. Electronic address:

Advances in electron cryo-microscopy (cryo-EM) now permit the structure determination of G protein-coupled receptors (GPCRs) coupled to heterotrimeric G proteins by single-particle imaging. A combination of G protein engineering and the development of antibodies that stabilise the heterotrimeric G protein facilitate the formation of stable GPCR-G protein complexes suitable for structural biology. Structures have been determined of GPCRs coupled to either heterotrimeric G proteins (G, G or G) or mini-G proteins (mini-G or mini-G) by single-particle cryo-EM and X-ray crystallography, respectively. Read More

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http://dx.doi.org/10.1016/j.mce.2019.02.006DOI Listing
May 2019
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Post-transcriptional markers associated with clinical complications in Type 1 and Type 2 diabetes mellitus.

Mol Cell Endocrinol 2019 Mar 26. Epub 2019 Mar 26.

Division of Clinical Immunology, Department of Medicine, Ribeirão Preto Medical School, University of São Paulo, 14048-900, Ribeirão Preto, SP, Brazil. Electronic address:

The delayed diagnosis and the inadequate treatment of diabetes increase the risk of chronic complications. The study of regulatory molecules such as miRNAs can provide expression profiles of diabetes and diabetes complications. We evaluated the mononuclear cell miRNA profiles of 63 Type 1 and Type 2 diabetes patients presenting or not microvascular complications, and 40 healthy controls, using massive parallel sequencing. Read More

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http://dx.doi.org/10.1016/j.mce.2019.03.008DOI Listing
March 2019
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Absence of the long noncoding RNA H19 results in aberrant ovarian STAR and progesterone production.

Mol Cell Endocrinol 2019 Mar 25. Epub 2019 Mar 25.

Department of Obstetrics, Gynecology, and Reproductive Sciences, Yale School of Medicine, New Haven, CT, USA. Electronic address:

The steroidogenic acute regulatory protein (STAR) governs the rate-limiting step in steroidogenesis, and its expression varies depending on the needs of the specific tissue. It is well known that tight control of steroid production is essential for multiple processes involved in reproduction. We recently showed that Star is regulated at the posttranscriptional level in vitro by H19 and let-7. Read More

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http://dx.doi.org/10.1016/j.mce.2019.03.009DOI Listing
March 2019
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The impact of Catechol-O-methyl transferase knockdown on the cell proliferation of hormone-responsive cancers.

Mol Cell Endocrinol 2019 May 21;488:79-88. Epub 2019 Mar 21.

Chapman University School of Pharmacy, Irvine, CA 92618, USA; School of Medicine, University of California, Irvine, CA, USA; College of Pharmacy, University of Sharjah, Sharjah 27272, United Arab Emirates.

Estrogen (E2) plays a central role in the development and progression of hormone-responsive cancers. Estrogen metabolites exhibit either stimulatory or inhibitory roles on breast and prostate cells. The catechol metabolite 4-hydroxyestradiol (4-OHE2) enhances cell proliferation, while 2-methoxyestradiol (2 ME) possesses anticancer activity. Read More

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http://dx.doi.org/10.1016/j.mce.2019.03.007DOI Listing
May 2019
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4.405 Impact Factor

Effects of in utero androgen excess and metformin treatment on hepatic functions.

Mol Cell Endocrinol 2019 Mar 14. Epub 2019 Mar 14.

Laboratorio de Fisio-patología Ovárica, Centro de Estudios Farmacológicos y Botánicos (CEFYBO), Consejo Nacional de Investigaciones Científicas y Tecnológicas (CONICET), Facultad de Medicina, Universidad de Buenos Aires (UBA), Paraguay 2155, CP1121, Ciudad Autónoma de Buenos Aires, Argentina. Electronic address:

This study aimed to evaluate the role of prenatal hyperandrogenization in liver functions and the extent of metformin as treatment. Pregnant rats were hyperandrogenized with subcutaneous testosterone (1mg/rat) between 16 and 19 of pregnancy. Prenatally hyperandrogenized (PH) female offspring displayed, at the adult life, two phenotypes; a PH irregular ovulatory phenotype (PHiov) and a PH anovulatory (PHanov) phenotype. Read More

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http://dx.doi.org/10.1016/j.mce.2019.03.006DOI Listing
March 2019
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SEIPIN overexpression in the liver may alleviate hepatic steatosis by influencing the intracellular calcium level.

Mol Cell Endocrinol 2019 May 12;488:70-78. Epub 2019 Mar 12.

State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, College of Animal Science and Technology, Guangxi University, PR China. Electronic address:

SEIPIN deficiency leads to a severe lipodystrophic phenotype with loss of fat tissue. Interestingly, SEIPIN knockout in non-adipocytes is reported to promote intracellular triacylglycerol (TG) accumulation. However, the underlying mechanisms remain unclear at present. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S03037207193008
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http://dx.doi.org/10.1016/j.mce.2019.03.005DOI Listing
May 2019
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Characterizing the complexity of Australian marsupial insulin-like growth factor 1 genes.

Authors:
Peter Rotwein

Mol Cell Endocrinol 2019 May 11;488:52-69. Epub 2019 Mar 11.

Department of Biomedical Sciences, Paul L. Foster School of Medicine, Texas Tech Health University Health Sciences Center, El Paso, TX 79905, USA.

Insulin-like growth factor 1 (IGF1) actions are essential for somatic growth and tissue repair. IGF1 gene regulation is controlled by many inputs, with growth hormone playing a major role. In most mammals, the 6-exon IGF1/Igf1 gene produces multiple transcripts via independent activity of its promoters plus alternative RNA splicing and differential polyadenylation. Read More

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http://dx.doi.org/10.1016/j.mce.2019.03.004DOI Listing

Subcellular localization and membrane topology of 17β-hydroxysteroid dehydrogenases.

Mol Cell Endocrinol 2018 Jul 7. Epub 2018 Jul 7.

Division of Molecular and Systems Toxicology, Department of Pharmaceutical Sciences, University of Basel, Klingelbergstrasse 50, 4056, Basel, Switzerland. Electronic address:

The 17β-hydroxysteroid dehydrogenases (17β-HSDs) comprise enzymes initially identified by their ability to interconvert active and inactive forms of sex steroids, a vital process for the tissue-specific control of estrogen and androgen balance. However, most 17β-HSDs have now been shown to accept substrates other than sex steroids, including bile acids, retinoids and fatty acids, thereby playing unanticipated roles in cell physiology. This functional divergence is often reflected by their different subcellular localization, with 17β-HSDs found in the cytosol, peroxisome, mitochondria, endoplasmic reticulum and in lipid droplets. Read More

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http://dx.doi.org/10.1016/j.mce.2018.07.003DOI Listing

GPCR photopharmacology.

Mol Cell Endocrinol 2019 May 9;488:36-51. Epub 2019 Mar 9.

MCS, Laboratory of Medicinal Chemistry, Institute for Advanced Chemistry of Catalonia (IQAC-CSIC), Barcelona, Spain. Electronic address:

New technologies for spatial and temporal remote control of G protein-coupled receptors (GPCRs) are necessary to unravel the complexity of GPCR signalling in cells, tissues and living organisms. An effective approach, recently developed, consists on the design of light-operated ligands whereby light-dependent GPCR activity regulation can be achieved. In this context, the use of light provides an advantage as it combines safety, easy delivery, high resolution and it does not interfere with most cellular processes. Read More

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http://dx.doi.org/10.1016/j.mce.2019.03.003DOI Listing

Asprosin impairs insulin secretion in response to glucose and viability through TLR4/JNK-mediated inflammation.

Mol Cell Endocrinol 2019 Apr 7;486:96-104. Epub 2019 Mar 7.

Department of Pharmacology, College of Medicine, Chung-Ang University, Seoul, South Korea. Electronic address:

Severe inflammation in the islets is observed in obese patients with type 2 diabetes. Inflammation in the islets is caused by obesity-induced serum free fatty acids. Asprosin is a fasting-induced adipokine, which contributes to hepatic glucose production. Read More

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http://dx.doi.org/10.1016/j.mce.2019.03.001DOI Listing
April 2019
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Maternal exposure to imazalil disrupts the endocrine system in F generation mice.

Mol Cell Endocrinol 2019 Apr 7;486:105-112. Epub 2019 Mar 7.

Department of Biotechnology, College of Biotechnology and Bioengineering, Zhejiang University of Technology, Hangzhou, 310014, China. Electronic address:

The fungicide imazalil (IMZ), an AR antagonist, has been linked to endocrine disruption in animals. Here, adult female C57BL/6 mice were administered IMZ through their drinking water at levels of 0, 0.025‰ and 0. Read More

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http://dx.doi.org/10.1016/j.mce.2019.03.002DOI Listing
April 2019
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CXCL12-regulated miR-370-3p functions as a tumor suppressor gene by targeting HMGA2 in nonfunctional pituitary adenomas.

Mol Cell Endocrinol 2019 May 7;488:25-35. Epub 2019 Mar 7.

The Dept. of Neurosurgery, The Second Affiliated Hospital of Zhejiang University School of Medicine, The City of Hangzhou, Zhejiang Province, PR China. Electronic address:

Silencing of noncoding genes within the imprinted DLK1-MEG3 locus is exclusive to human nonfunctional pituitary adenomas (NFPAs), but the exact mechanism is still unclear. This study was designed to demonstrate the impact of CXCL12 on the expression of miRNAs within this locus and phenotypic alterations of NFPAs. Human NFPA samples were collected for screening differentially expressed miRNAs by CXCL12. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S03037207193006
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http://dx.doi.org/10.1016/j.mce.2019.02.020DOI Listing
May 2019
5 Reads
4.405 Impact Factor

GPCR interaction as a possible way for allosteric control between receptors.

Mol Cell Endocrinol 2019 Apr 5;486:89-95. Epub 2019 Mar 5.

Institut de Génomique Fonctionnelle, Université de Montpellier, CNRS, INSERM, Montpellier, France.

For more than twenty years now, GPCR dimers and larger oligomers have been the subject of intense debates. Evidence for a role of such complexes in receptor trafficking to and from the plasma membrane have been provided. However, one main issue is of course to determine whether or not such a phenomenon can be responsible for an allosteric and reciprocal control (allosteric control) of the subunits. Read More

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http://dx.doi.org/10.1016/j.mce.2019.02.019DOI Listing
April 2019
2 Reads

Relaxin in liver transplantation: A personal perspective.

Mol Cell Endocrinol 2019 May 3;487:75-79. Epub 2019 Mar 3.

The Dumont-UCLA Transplant Center, Department of Surgery, Division of Liver and Pancreas Transplantation, David Geffen School of Medicine at University of California, Los Angeles, CA, 90095, USA. Electronic address:

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http://dx.doi.org/10.1016/j.mce.2019.02.022DOI Listing
May 2019
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Editorial to the mini-review series on relaxin, related peptides and receptors?

Authors:
Thomas Klonisch

Mol Cell Endocrinol 2019 May 2;487. Epub 2019 Mar 2.

Department of Human Anatomy and Cell Science, Rady Faculty of Health Sciences, Max Rady College of Medicine, University of Manitoba, Manitoba, Canada. Electronic address:

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http://dx.doi.org/10.1016/j.mce.2019.02.021DOI Listing

Tibolone attenuates inflammatory response by palmitic acid and preserves mitochondrial membrane potential in astrocytic cells through estrogen receptor beta.

Mol Cell Endocrinol 2019 Apr 26;486:65-78. Epub 2019 Feb 26.

Departamento de Nutrición y Bioquímica, Facultad de Ciencias, Pontificia Universidad Javeriana, Bogotá D.C, Colombia. Electronic address:

Palmitic acid (PA) induces several metabolic and molecular changes in astrocytes, and, it is involved in pathological conditions related to neurodegenerative diseases. Previously, we demonstrated that tibolone, a synthetic steroid with estrogenic, progestogenic and androgenic actions, protects cells from mitochondrial damage and morphological changes induced by PA. Here, we have evaluated which estrogen receptor is involved in protective actions of tibolone and analyzed whether tibolone reverses gene expression changes induced by PA. Read More

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http://dx.doi.org/10.1016/j.mce.2019.02.017DOI Listing
April 2019
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Low-dose environmental endocrine disruptors, increase aromatase activity, estradiol biosynthesis and cell proliferation in human breast cells.

Mol Cell Endocrinol 2019 Apr 26;486:55-64. Epub 2019 Feb 26.

Molecular and Cellular Medicine Group, School of Biological Sciences, University of Reading, Reading, Berkshire, RG6 6UB, United Kingdom.

Background: Phenolic endocrine-disrupting compounds (EDCs) have long been suspected of increasing human breast cancer risk, via aromatase up-regulation; however, the metabolic effects upon aromatase in human breast cells exposed to environmentally relevant concentrations of phenolic compounds, have not been addressed.

Objectives: To examine the mechanistic responses of aromatase CYP19A1 mRNA, aromatase activity, estradiol biosynthesis and cellular proliferation, in three human breast cell lines, exposed to seven phenolic compounds, at environmentally relevant concentrations.

Methods: MCF-7 and ZR-75-1 breast cancer cells, and HMF3A breast fibroblasts were treated with specific concentrations of p,p'-DDT, methoxychlor, benzophenone-2, bisphenol A, bisphenol S, 4-phenylphenol and n-butylparaben, with and without the presence of aromatase inhibitors and estrogen receptor inhibitors. Read More

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http://dx.doi.org/10.1016/j.mce.2019.02.016DOI Listing
April 2019
2 Reads

Knockout of insulin-degrading enzyme leads to mice testicular morphological changes and impaired sperm quality.

Mol Cell Endocrinol 2019 Apr 23;486:11-17. Epub 2019 Feb 23.

Department of Microscopy, Laboratory of Cell Biology and Unit for Multidisciplinary Research in Biomedicine (UMIB), Institute of Biomedical Sciences Abel Salazar (ICBAS), University of Porto, Porto, Portugal. Electronic address:

Insulin-degrading enzyme (IDE) is a zinc metalloprotease responsible for degrading and inactivating several bioactive peptides, including insulin. Individuals without this enzyme or with a loss-of-function mutation in the gene that codifies it, present hyperinsulinemia. In addition, impairment of IDE-mediated insulin clearance is associated with the development of metabolic diseases, namely prediabetes. Read More

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http://dx.doi.org/10.1016/j.mce.2019.02.011DOI Listing
April 2019
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Adrenal androgens rescue prostatic dihydrotestosterone production and growth of prostate cancer cells after castration.

Mol Cell Endocrinol 2019 Apr 23;486:79-88. Epub 2019 Feb 23.

Department of Urology, Roswell Park Comprehensive Cancer Center, Buffalo, NY 14263, USA.

Adrenal androgens dehydroepiandrosterone (DHEA) and DHEA-sulfate (DHEAS) are potential substrates for intracrine production of testosterone (T) and dihydrotestosterone (DHT), or directly to DHT, by prostate cancer (PCa) cells. Production of DHT from DHEAS and DHEA, and the role of steroid sulfatase (STS), were evaluated ex vivo using fresh human prostate tissue and in vitro using human PCa cell lines. STS was expressed in benign prostate tissue and PCa tissue. Read More

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http://dx.doi.org/10.1016/j.mce.2019.02.018DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6438375PMC
April 2019
1 Read
4.405 Impact Factor

Expression of genes controlling steroid metabolism and action in granulosa-lutein cells of women with polycystic ovaries.

Mol Cell Endocrinol 2019 Apr 22;486:47-54. Epub 2019 Feb 22.

Institute of Reproductive and Developmental Biology, Imperial College London, Hammersmith Hospital, London, UK.

Introduction: Aberrant function of granulosa cells has been implicated in the pathophysiology of PCOS.

Materials & Methods: Granulosa lutein (GL) cells were collected during oocyte retrieval for IVF/ICSI. RT-qPCR was used to compare gene expression between 12 control women, 12 with ovulatory PCO and 12 with anovulatory PCOS. Read More

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http://dx.doi.org/10.1016/j.mce.2019.02.015DOI Listing

Human granulosa cells function as innate immune cells executing an inflammatory reaction during ovulation: a microarray analysis.

Mol Cell Endocrinol 2019 Apr 22;486:34-46. Epub 2019 Feb 22.

Herlev Fertility Clinic, University Hospital of Copenhagen, Herlev and Gentofte Hospital, Herlev Ringvej 75, 2730, Herlev, Denmark.

Ovulation has been compared to a local inflammatory reaction. We performed an in silico study on a unique, PCR validated, transcriptome microarray study to evaluate if known inflammatory mechanisms operate during ovulation. The granulosa cells were obtained in paired samples at two different time points during ovulation (just before and 36 hours after ovulation induction) from nine women receiving fertility treatment. Read More

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http://dx.doi.org/10.1016/j.mce.2019.02.014DOI Listing
April 2019
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24S-hydroxycholesterol affects redox homeostasis in human glial U-87 MG cells.

Mol Cell Endocrinol 2019 Apr 23;486:25-33. Epub 2019 Feb 23.

Dipartimento di Biologia, Università di Napoli Federico II, Complesso Universitario Monte Sant'Angelo, Via Cinthia, I-80126, Napoli, Italy. Electronic address:

The cholesterol metabolite 24(S)-hydroxycholesterol (24S-OHC) allows cholesterol excretion from the brain and was suggested to be critically involved in physiological as well as neurodegenerative processes. It induces on human neuronal cell cultures a dose dependent toxicity associated with increased reactive oxygen species production. Since glial cells play a key role in assisting neuronal function, here we investigated the effects of increased concentrations of 24S-OHC on a glial cell model (human glioblastoma U-87 MG cells). Read More

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http://dx.doi.org/10.1016/j.mce.2019.02.013DOI Listing

Functional genomic analysis of the human receptive endometrium transcriptome upon administration of mifepristone at the time of follicle rupture.

Mol Cell Endocrinol 2019 Apr 21;485:88-96. Epub 2019 Feb 21.

Departmento de Biología de La Reproducción Dr. Carlos Gual Castro, Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán, Avenida Vasco de Quiroga No. 15, Ciudad de México, 14080, México. Electronic address:

The aim of this study was to analyze the effects of progesterone withdrawal on gene transcription in receptive endometrium by the administration of a single dose of 50 mg of the anti-progesterone receptor mifepristone (MFP) at the time of follicle rupture (FR). Six volunteer ovulatory women were studied, taking endometrial biopsies of three control and three MFP-treated women on days LH+2 (C-LH+2) and LH+7 (T-MFP), respectively. The biopsies were prepared for RNA isolation or histological and immunohistochemistry studies. Read More

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http://dx.doi.org/10.1016/j.mce.2019.02.010DOI Listing

MicroRNA-9 affects isolated ovarian granulosa cells proliferation and apoptosis via targeting vitamin D receptor.

Mol Cell Endocrinol 2019 Apr 19;486:18-24. Epub 2019 Feb 19.

Department of Obstetrics and Gynecology, Henan Provincial People's Hospital, Zhengzhou, China.

MicroRNAs (miRNAs or miRs)-9 expression was reported to be upregulated in the follicular fluid of patients with polycystic ovary syndrome (PCOS). However, whether miR-9 affects ovarian dysfunction of PCOS and the related mechanisms are still unclear. Here we detected miR-9 and vitamin D receptor (VDR) expression in women with PCOS and controls, and investigated whether miR-9 affects ovarian granulosa cells (GCs) proliferation and apoptosis by targeting VDR. Read More

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http://dx.doi.org/10.1016/j.mce.2019.02.012DOI Listing
April 2019
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TNF-alpha inhibits pregnancy-adapted Ca signaling in uterine artery endothelial cells.

Mol Cell Endocrinol 2019 May 16;488:14-24. Epub 2019 Feb 16.

Perinatal Research Laboratories, Department of Obstetrics and Gynecology, School Medicine and Public Health, University of Wisconsin-Madison, 7E Unity Point Health-Meriter Hospital, 202 South Park Street, Madison, WI, 53715, USA. Electronic address:

Enhancement of vasodilation of uterine arteries during pregnancy occurs through increased connexin (Cx)43 gap junction (GJ) communication supporting more frequent and sustained Ca 'bursts'. Such adaptation is lacking in subjects with preeclampsia (PE). Here we show TNF-alpha, commonly increased in PE subjects, inhibits Cx43 function and Ca bursts in pregnancy-derived ovine uterine artery endothelial cells (P-UAEC) via Src and MEK/ERK phosphorylation of Cx43, and this can be reversed by PP2 or U0126. Read More

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http://dx.doi.org/10.1016/j.mce.2019.02.008DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6475486PMC

Relaxin and fibrosis: Emerging targets, challenges, and future directions.

Mol Cell Endocrinol 2019 May 14;487:66-74. Epub 2019 Feb 14.

Cardiovascular Disease Theme, Monash Biomedicine Discovery Institute and Department of Pharmacology, Monash University, Clayton, VIC, Australia; Central Clinical School, Monash University, Prahran, VIC, Australia. Electronic address:

The peptide hormone relaxin is well-known for its anti-fibrotic actions in several organs, particularly from numerous studies conducted in animals. Acting through its cognate G protein-coupled receptor, relaxin family peptide receptor 1 (RXFP1), serelaxin (recombinant human relaxin) has been shown to consistently inhibit the excessive extracellular matrix production (fibrosis) that results from the aberrant wound-healing response to tissue injury and/or chronic inflammation, and at multiple levels. Furthermore, it can reduce established scarring by promoting the degradation of aberrant extracellular matrix components. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S03037207193004
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http://dx.doi.org/10.1016/j.mce.2019.02.005DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6475456PMC
May 2019
4 Reads

Emerging roles for the relaxin/RXFP1 system in cancer therapy.

Mol Cell Endocrinol 2019 May 11;487:85-93. Epub 2019 Feb 11.

Department of Human Anatomy and Cell Science, College of Medicine, University of Manitoba, Winnipeg, Canada. Electronic address:

A role for the hormone relaxin in cancer was described well before the receptor was identified. Relaxin predominantly increases the growth and invasive potential in cancers of different origins. However, relaxin was also shown to promote cell differentiation and to act in a dose-and time-dependent manner in different cancer cell models used. Read More

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http://dx.doi.org/10.1016/j.mce.2019.02.001DOI Listing
May 2019
3 Reads

Galectin-3 plays an important role in endometriosis development and is a target to endometriosis treatment.

Mol Cell Endocrinol 2019 Apr 10;486:1-10. Epub 2019 Feb 10.

Morphological Sciences Program, Biomedical Sciences Institute, Federal University of Rio de Janeiro - UFRJ, Rio de Janeiro, RJ, Brazil. Electronic address:

This study aimed to analyze galectin-3 importance in endometriotic lesions development and the effect of recombinant Gal-3 carbohydrate recognition domain (Gal3C) in experimental endometriosis treatment. Experimental endometriosis was induced in WT and Gal-3-/- mice. Initially developed lesions were macroscopically and histologically analyzed, including immunohistochemical analysis. Read More

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http://dx.doi.org/10.1016/j.mce.2019.02.007DOI Listing
April 2019
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Effects of stanniocalcin hormones on rat brown adipose tissue metabolism under fed and fasted conditions.

Mol Cell Endocrinol 2019 Apr 7;485:81-87. Epub 2019 Feb 7.

Department of Physiology, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil. Electronic address:

In this study we determined the effect of fed and fasting (48 h) states on the expression of stanniocalcin-1 (Stc1) and stanniocalcin-2 (Stc2) in rat brown adipose tissue (BAT), as well as the in vitro effects of human stanniocalcin 1 and 2 (hSTC-1 and hSTC-2) hormones on lipid and glucose metabolism. In addition, lactate, glycogen levels and hexokinase (HK) activity were determined. In fasting Stc2 expression increased markedly. Read More

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http://dx.doi.org/10.1016/j.mce.2019.02.004DOI Listing

Binding kinetics of ligands acting at GPCRs.

Mol Cell Endocrinol 2019 Apr 8;485:9-19. Epub 2019 Feb 8.

Cell Signalling and Pharmacology Research Group, Division of Physiology, Pharmacology and Neuroscience, School of Life Sciences, University of Nottingham, Nottingham, UK; Centre of Membrane Proteins and Receptors (COMPARE), University of Birmingham and University of Nottingham, Midlands, UK. Electronic address:

The influence of drug-receptor binding kinetics has often been overlooked during the development of new therapeutics that target G protein-coupled receptors (GPCRs). Over the last decade there has been a growing understanding that an in-depth knowledge of binding kinetics at GPCRs is required to successfully target this class of proteins. Ligand binding to a GPCR is often not a simple single step process with ligand freely diffusing in solution. Read More

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http://dx.doi.org/10.1016/j.mce.2019.01.018DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6406023PMC
April 2019
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ETV5 regulates GOAT/ghrelin system in an mTORC1-dependent manner.

Mol Cell Endocrinol 2019 Apr 5;485:72-80. Epub 2019 Feb 5.

Center for Diabetes, Obesity and Metabolism, Department of Physiology, Shenzhen University Health Science Center, Shenzhen, Guangdong province, 518000, China; Department of Physiology and Pathophysiology, School of Basic Science, Peking University Health Science Center, Beijing, 100191, China. Electronic address:

Ghrelin, a 28 amino acid peptide hormone, regulates multiple important metabolic functions. Its acylation by ghrelin-O-acyl-transferase enzyme (GOAT) is required for binding to and activating its receptor, the growth hormone secretagogue receptor 1a. Mechanism underlying the regulation of GOAT and acyl ghrelin remains unclear. Read More

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http://dx.doi.org/10.1016/j.mce.2019.02.003DOI Listing
April 2019
4.405 Impact Factor

Clinical applications of small molecule inhibitors of Pregnane X receptor.

Mol Cell Endocrinol 2019 Apr 3;485:61-71. Epub 2019 Feb 3.

Basic Sciences, Kansas City University of Medicine and Biosciences, Joplin, MO, USA. Electronic address:

The canonical effect of Pregnane X Receptor (PXR, NR1I2) agonism includes enhanced hepatic uptake and a concomitant increase in the first-pass metabolism and efflux of drugs in mammalian liver and intestine. In patients undergoing combination therapy, PXR-mediated gene regulation represents the molecular basis of numerous food-drug, herb-drug, and drug-drug interactions. Moreover, PXR activation promotes chemotherapeutic resistance in certain malignancies. Read More

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http://dx.doi.org/10.1016/j.mce.2019.02.002DOI Listing
April 2019
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The impact of sensory and motor enrichment on the epigenetic control of steroidogenic-related genes in rat hippocampus.

Mol Cell Endocrinol 2019 Apr 2;485:44-53. Epub 2019 Feb 2.

Departamento de Bioquímica Clínica y Cuantitativa, Facultad de Bioquímica y Ciencias Biológicas, Universidad Nacional del Litoral, Santa Fe, Argentina; Instituto de Salud y Ambiente del Litoral(ISAL), Facultad de Bioquímica y Ciencias Biológicas, Universidad Nacional del Litoral-CONICET, Santa Fe, Argentina. Electronic address:

In the present study, we analyzed the effects of a short-term environmental enrichment on the mRNA expression and DNA methylation of steroidogenic enzymes in the hippocampus. Thus, young adult (80-day-old) and middle-aged (350-day-old) Wistar female rats were exposed to sensory (SE) or motor (ME) enrichment during 10 days and compared to animals housed under standard conditions. SE was provided by an assortment of objects that included plastic tubes and toys; for ME, rodent wheels were provided. Read More

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http://dx.doi.org/10.1016/j.mce.2019.01.025DOI Listing

Regulation of Gpr173 expression, a putative phoenixin receptor, by saturated fatty acid palmitate and endocrine-disrupting chemical bisphenol A through a p38-mediated mechanism in immortalized hypothalamic neurons.

Mol Cell Endocrinol 2019 Apr 1;485:54-60. Epub 2019 Feb 1.

Department of Physiology, University of Toronto, Toronto, ON, Canada; Departments of Obstetrics and Gynaecology and Medicine, University of Toronto, Toronto, ON, Canada. Electronic address:

GPR173 is a highly conserved G protein coupled receptor associated with the hypothalamic-pituitary-gonadal reproductive axis. It is expressed in the brain and ovaries, however considerable knowledge about its function remains unknown. One putative ligand for this receptor is phoenixin (PNX), a newly identified reproductive peptide involved in hypothalamic coordination of the estrous cycle. Read More

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http://dx.doi.org/10.1016/j.mce.2019.01.026DOI Listing
April 2019
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Rapid effect of bisphenol A on glutamate-induced Ca influx in hippocampal neurons of rats.

Mol Cell Endocrinol 2019 Apr 30;485:35-43. Epub 2019 Jan 30.

Chemistry and Life Sciences College, Key Laboratory of Wildlife Biotechnology and Conservation and Utilization of Zhejiang Province, Zhejiang Provincial Key Laboratory of Ecology, Zhejiang Normal University, PR China. Electronic address:

Intracellular Ca signaling plays an essential role in synaptic plasticity. This study examined the effect of BPA on concentration of intracellular Ca ([Ca]) by measuring fluorescence intensity of Ca in hippocampal neurons in vitro. The results showed that BPA for 30 min exerted dose-dependently dual effects on glutamate-elevated [Ca]: BPA at 1-10 μM suppressed but at 1-100 nM enhanced glutamate-raised [Ca]. Read More

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http://dx.doi.org/10.1016/j.mce.2019.01.024DOI Listing

The structural basis of the arrestin binding to GPCRs.

Mol Cell Endocrinol 2019 Mar 28;484:34-41. Epub 2019 Jan 28.

Department of Pharmacology, Vanderbilt University, Nashville, TN, 37232, USA.

G protein-coupled receptors (GPCRs) are the largest family of signaling proteins targeted by more clinically used drugs than any other protein family. GPCR signaling via G proteins is quenched (desensitized) by the phosphorylation of the active receptor by specific GPCR kinases (GRKs) followed by tight binding of arrestins to active phosphorylated receptors. Thus, arrestins engage two types of receptor elements: those that contain GRK-added phosphates and those that change conformation upon activation. Read More

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http://dx.doi.org/10.1016/j.mce.2019.01.019DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6377262PMC

Allosteric small molecule modulators of nuclear receptors.

Mol Cell Endocrinol 2019 Apr 28;485:20-34. Epub 2019 Jan 28.

Laboratory of Chemical Biology, Department of Biomedical Engineering and Institute for Complex Molecular Systems, Technische Universiteit Eindhoven, Den Dolech 2, 5612AZ, Eindhoven, the Netherlands. Electronic address:

Nuclear Receptors (NRs) are multi-domain proteins, whose natural regulation occurs via ligands for a classical, orthosteric, binding pocket and via intra- and inter-domain allosteric mechanisms. Allosteric modulation of NRs via synthetic small molecules has recently emerged as an interesting entry to address the need for small molecules targeting NRs in pathology, via novel modes of action and with beneficial profiles. In this review the general concept of allosteric modulation in drug discovery is first discussed, serving as a background and inspiration for NRs. Read More

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http://dx.doi.org/10.1016/j.mce.2019.01.022DOI Listing

Modulation of nuclear receptor function: Targeting the protein-DNA interface.

Mol Cell Endocrinol 2019 Mar 28;484:1-14. Epub 2019 Jan 28.

Brazilian Biosciences National Laboratory (LNBio), Brazilian Center for Research in Energy and Materials (CNPEM), Campinas, SP, 13083-970, Brazil. Electronic address:

Nuclear receptors (NRs) are a superfamily of ligand-dependent transcription factors that modulate several biological processes. Traditionally, modulation of NRs has been focused on the development of ligands that recognize and bind to the ligand binding domain (LBD), resulting in activation or repression of transcription through the recruitment of coregulators. However, for more severe diseases, such as breast and prostate cancer, the conventional treatment addressing LBD modulation is not always successful, due to tumor resistance. Read More

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http://dx.doi.org/10.1016/j.mce.2019.01.023DOI Listing
March 2019
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Japanese medaka as a model for studying the relaxin family genes involved in neuroendocrine regulation: Insights from the expression of fish-specific rln3 and insl5 and rxfp3/4-type receptor paralogues.

Mol Cell Endocrinol 2019 May 28;487:2-11. Epub 2019 Jan 28.

Department of Biology, The University of Winnipeg, Winnipeg, MB, Canada; Department of Biology, The University of Manitoba, Winnipeg, MB, Canada. Electronic address:

The goal of this paper is to establish Japanese medaka (Oryzias latipes) as a model for relaxin family peptide research, particularly for studying the functions of RLN3 and INSL5, hormones playing roles in neuroendocrine regulation. Medaka, like other teleosts, retained duplicate copies of rln3, insl5 and their rxfp3/4-type receptors following fish-specific whole genome duplication (WGD) and paralogous copies of these genes may have sub-functionalised providing an intuitive model for teasing apart the pleiotropic roles of the corresponding genes in mammals. To this end, we provide experimental evidence for the expression of the relaxin family genes in medaka that had previously only been identified in-silico, confirm the gene structure of five of the ligand genes, characterise gene expression across multiple tissues and during embryonic development, perform in situ hybridization with anti-sense insl5a on embryos and in adult brain and intestinal samples, and compare these results to the data available in zebrafish. Read More

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http://dx.doi.org/10.1016/j.mce.2019.01.017DOI Listing
May 2019
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Nanobodies: New avenues for imaging, stabilizing and modulating GPCRs.

Mol Cell Endocrinol 2019 Mar 26;484:15-24. Epub 2019 Jan 26.

Amsterdam Institute for Molecules, Medicines and Systems (AIMMS), Division of Medicinal Chemistry, Faculty of Sciences, Vrije Universiteit, De Boelelaan 1108, 1081 HZ, Amsterdam, the Netherlands. Electronic address:

The family of G protein-coupled receptors (GPCRs) is the largest class of membrane proteins and an important drug target due to their role in many (patho)physiological processes. Besides small molecules, GPCRs can be targeted by biologicals including antibodies and antibody fragments. This review describes the use of antibodies and in particular antibody fragments from camelid-derived heavy chain-only antibodies (nanobodies/VHHs/sdAbs) for detecting, stabilizing, modulating and therapeutically targeting GPCRs. Read More

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http://dx.doi.org/10.1016/j.mce.2019.01.021DOI Listing
March 2019
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