1,856 results match your criteria Molecular and Cellular Probes [Journal]


EvaGreen-based real-time PCR assay for sensitive detection of enzootic nasal tumor virus 2.

Mol Cell Probes 2019 Feb 13. Epub 2019 Feb 13.

College of Animal Science and Technology, Southwest University, Chongqing, 400715, China. Electronic address:

Enzootic nasal tumor virus 2 (ENTV-2), the aetiological agent of enzootic nasal adenocarcinoma in goats, is prevalent in China; resulting in substantial economic losses to the goat-breeding industry. Therefore, it is necessary to establish an efficient detection method for the diagnosis and prevention of ENTV-2 infection. More recently, EvaGreen is emerging as a novel alternative fluorescent dye for quantitative real-time PCR because of its low cost, specific amplification and high resolution. Read More

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http://dx.doi.org/10.1016/j.mcp.2019.02.003DOI Listing
February 2019

Development of a SYBR green I-based duplex real-time fluorescence quantitative PCR assay for the simultaneous detection of porcine epidemic diarrhea virus and porcine circovirus 3.

Mol Cell Probes 2019 Feb 5. Epub 2019 Feb 5.

College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengdong New District Longzi Lake 15#, Zhengzhou, 450046, Henan Province, People's Republic of China; Zhengzhou Major Pig Disease Prevention and Control Laboratory, Zhengzhou, 450046, Henan Province, People's Republic of China. Electronic address:

The development of a rapid, specific, and sensitive SYBR Green I-based duplex real-time quantitative PCR assay is described for the simultaneous detection of porcine epidemic diarrhea virus (PEDV) and porcine circovirus type 3 (PCV3). The assay specifically detected PEDV and PCV3, with no fluorescence detected for other non-targeted pig pathogens. The assay showed a good linear relationship, and the limits of detection for this assay were 34. Read More

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http://dx.doi.org/10.1016/j.mcp.2019.02.002DOI Listing
February 2019
1 Read

MiR-486-5p inhibits the proliferation of leukemia cells and induces apoptosis through targeting FOXO1.

Mol Cell Probes 2019 Feb 4. Epub 2019 Feb 4.

Department of Hematology, The First Affiliated Hospital of Xi'an Medical University, China. Electronic address:

Aim: Studies have reported that micro (miR)-486-5p plays a crucial part in the progression of leukemia, however, to the best of our knowledge, few studies have been conducted on its mechanism in leukemia. In this study, the mechanism of miR-486-5p in leukemia cells was pointed out and its possible target genes were analyzed for the purpose of providing new therapeutic strategies for treating leukemia patients.

Methods: MiRNA expression of Leukemia cells (K562, Kasumi-1, and THP-1) and primary leukocytes was detected by Real-time Quantitative polymerase chain reaction(qPCR). Read More

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https://linkinghub.elsevier.com/retrieve/pii/S08908508193000
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http://dx.doi.org/10.1016/j.mcp.2019.02.001DOI Listing
February 2019
2 Reads

MicroRNA-224 impairs adipogenic differentiation of bovine preadipocytes by targeting LPL.

Mol Cell Probes 2019 Jan 28. Epub 2019 Jan 28.

College of Animal Science and Technology, National Beef Cattle Improvement Center, Northwest A&F University, Yangling, Shaanxi, China. Electronic address:

In this study, the potential functions of miR-224 in regulating adipogenic differentiation were explored in bovine preadipocytes. Comparative transcriptome analysis between castrated male cattle with increased intramuscular fat (IMF) and intact male cattle revealed that miR-224 and LPL were abnormally expressed, correlating negatively, and LPL was a predicted target of miR-224. A dual luciferase reporter assay confirmed the negative targeting regulatory relationship between miR-224 and LPL. Read More

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http://dx.doi.org/10.1016/j.mcp.2019.01.005DOI Listing
January 2019

Downregulated low-density lipoprotein receptor-related protein 6 induces the maldevelopment of extravillous trophoblast via Wnt/β-catenin signaling pathway.

Mol Cell Probes 2019 Jan 24. Epub 2019 Jan 24.

Department of Obstetrics, The Affiliated Yantai Yuhuangding Hospital of Qingdao University, China. Electronic address:

Preeclampsia (PE), a special type of hypertensive disorder complicating pregnancy (HDCP), is highly associated with the migratory and invasive capacity of extravillous trophoblasts (EVTs). Here, we aimed to study the functions of low-density lipoprotein receptor-related protein 6 (LRP6) in PE pathogenesis. A comparative analysis of cellular gene expressions between placenta tissues collected from PE patients and normal pregnant women showed that the expressions of LRP6, β-catenin and matrix metallopeptidases/TIMP metallopeptidase inhibitors (MMPs/TIMPs) ratio in placentas of PE patients were much lower than the normal. Read More

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http://dx.doi.org/10.1016/j.mcp.2019.01.004DOI Listing
January 2019

HADHA and HADHB gene associated phenotypes - Identification of rare variants in a patient cohort by Next Generation Sequencing.

Mol Cell Probes 2019 Jan 22. Epub 2019 Jan 22.

Medical Genetics Center, Munich, Germany; Department of Neurology, Friedrich-Baur-Institute, Klinikum der Ludwig-Maximilians-University, Munich, Germany. Electronic address:

The heterooctameric mitochondrial trifunctional protein (MTP), composed of four α- and β-subunits harbours three enzymes that each perform a different function in mitochondrial fatty acid β-oxidation. Pathogenic variants in the MTP genes (HADHA and HADHB) cause MTP deficiency, a rare autosomal recessive metabolic disorder characterized by phenotypic heterogeneity ranging from severe, early-onset, cardiac disease to milder, later-onset, myopathy and neuropathy. Since metabolic myopathies and neuropathies are a group of rare genetic disorders and their associated muscle symptoms may be subtle, the diagnosis is often delayed. Read More

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http://dx.doi.org/10.1016/j.mcp.2019.01.003DOI Listing
January 2019
1 Read

Novel dual-color drug screening model for GLUT4 translocation in adipocytes.

Mol Cell Probes 2019 Feb 11;43:6-12. Epub 2019 Jan 11.

State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, 650201, China; The General Hospital of Ningxia Medical University, Department of Beijing National Biochip Research Center Sub-Center in Ningxia, Yinchuan, 750004, China. Electronic address:

Insulin-responsive glucose transporter type 4 (GLUT4) translocation plays a major role in controlling glucose uptake in adipose tissue and muscle, maintaining homeostasis and preventing hyperglycemia. Screening for chemicals enhancing GLUT4 translocation is an approach for identifying hits of drug development for type 2 diabetes. Here we developed a novel functional dual-color probe, pHluorin-GLUT4-mOrange2, and constructed 3T3-L1 adipocytes based screening system to simply and efficiently screen new compounds stimulating GLUT4 translocation. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S08908508183027
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http://dx.doi.org/10.1016/j.mcp.2019.01.002DOI Listing
February 2019
5 Reads

Next generation sequencing and imprinting disorders: Current applications and future perspectives: Lessons from Silver-Russell syndrome.

Mol Cell Probes 2019 Jan 2. Epub 2019 Jan 2.

Institute of Human Genetics, University Hospital, Technical University RWTH, Aachen, Germany. Electronic address:

Imprinting Disorders are a group of rare diseases with overlapping phenotypes which are associated with similar molecular changes and affect imprinted chromosomal regions. Clinical features mainly occur prenatally or in childhood, but have a severe lifelong impact on health. Due to their clinical and molecular heterogeneity, the diagnosis of imprinting disorders is often challenging and requires testing of a broad spectrum of genomic variants and aberrant methylation of imprinted loci (epimutations). Read More

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http://dx.doi.org/10.1016/j.mcp.2018.12.007DOI Listing
January 2019

A real-time recombinase polymerase amplification assay for the rapid detection of Vibrio harveyi.

Mol Cell Probes 2019 Jan 3. Epub 2019 Jan 3.

Ningbo Institute of Oceanography, Ningbo, 315832, China.

Vibrio harveyi is a pathogen that infects fish and shellfish worldwide, causing severe economic losses for the aquaculture industry. As the early diagnosis of V. harveyi infection is crucial to disease surveillance and prevention in cultured marine animals, a fast and accurate method to detect V. Read More

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http://dx.doi.org/10.1016/j.mcp.2019.01.001DOI Listing
January 2019
5 Reads

Direct comparison of QIAamp DSP Virus Kit and QIAamp Circulating Nucleic Acid Kit regarding cell-free fetal DNA isolation from maternal peripheral blood.

Mol Cell Probes 2019 Feb 22;43:13-19. Epub 2018 Dec 22.

Medical Research and Education Center, Lomonosov Moscow State University, Lomonosovsky Prospect, 27/10, 119192, Moscow, Russia. Electronic address:

Background: Blood of pregnant women contains cell-free fetal DNA (cffDNA), which is widely used in non-invasive prenatal diagnosis. The modern laboratory equipment market provides huge variety of commercial kits for isolation of circulating nucleic acids, but unfortunately none of them are standardized for isolation of cffDNA, which is a crucial step for success of subsequent analysis.

Aim: To compare DSPVK and CNAK in terms of cffDNA, cell-free total DNA (cftDNA) yield and resulting cffDNA fraction, as well as to try to explain the possible difference between the efficacy of these kits. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S08908508183025
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http://dx.doi.org/10.1016/j.mcp.2018.12.006DOI Listing
February 2019
12 Reads

Rapid detection of Cucumber green mottle mosaic virus by reverse transcription recombinase polymerase amplification.

Mol Cell Probes 2019 Feb 22;43:84-85. Epub 2018 Dec 22.

Institute of Eco-Environment and Plant Protection, Shanghai Key Laboratory of Protection Horticultural Technology, Shanghai Academy of Agricultural Sciences, Shanghai, 201403, China. Electronic address:

Reverse transcription recombinase polymerase amplification (RT-RPA) for detection of Cucumber green mottle mosaic virus (CGMMV) was developed in this study. It was found to be specific, with a limit of detection of 0.5 pg of total CGMMV RNA. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S08908508183030
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http://dx.doi.org/10.1016/j.mcp.2018.12.005DOI Listing
February 2019
3 Reads

Multiplex SYBR Green and duplex TaqMan real-time PCR assays for the detection of Photorhabdus Insect-Related (Pir) toxin genes pirA and pirB.

Mol Cell Probes 2019 Feb 18;43:20-28. Epub 2018 Dec 18.

Aquaculture Pathology Laboratory, School of Animal and Comparative Biomedical Sciences, The University of Arizona, AZ. 85721, USA. Electronic address:

Acute hepatopancreatic necrosis disease (AHPND), also known as Early mortality syndrome (EMS), is a recently emerged lethal disease that has caused major economic losses in shrimp aquaculture. The etiologic agents are Vibrio spp. that carry Photorhabdus Insect-Related (Pir) toxin genes pirA and pirB. Read More

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http://dx.doi.org/10.1016/j.mcp.2018.12.004DOI Listing
February 2019

A novel fluorometric method for inorganic pyrophosphatase detection based on G-quadruplex-thioflavin T.

Mol Cell Probes 2019 Feb 17;43:29-33. Epub 2018 Dec 17.

School of Life Sciences, Central South University, Changsha, 410013, China.

In this paper, we propose a fluorometric approach for the highly sensitive detection of inorganic pyrophosphatase (PPase) based on G-quadruplex-thioflavin T (ThT). In the absence of PPase, Cu can coordinate with pyrophosphate (PPi) to generate a Cu/PPi complex. Then the G-rich sequence folds into the G-quadruplex structure, which can combine with ThT to generate a remarkable fluorescent signal. Read More

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http://dx.doi.org/10.1016/j.mcp.2018.12.003DOI Listing
February 2019

Ginsenoside Rb1 and mitochondria: A short review of the literature.

Mol Cell Probes 2019 Feb 4;43:1-5. Epub 2018 Dec 4.

Institute of Medicinal Plant Development, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing, 100193, China. Electronic address:

Mitochondria play a central role in various critical cellular processes, including energy synthesis, energy supply and apoptosis. Panax notoginseng, a commonly used traditional Chinese medicine, has various pharmacological effects on the human body. Ginsenosides are representative bioactive components of P. Read More

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http://dx.doi.org/10.1016/j.mcp.2018.12.001DOI Listing
February 2019
1 Read

Application of random amplified polymorphism DNA and 16S-23S rDNA intergenic spacer polymerase chain reaction-restriction fragment length polymorphism to predict major Streptococcus suis clonal complexes isolated from humans and pigs.

Mol Cell Probes 2019 Feb 5;43:34-39. Epub 2018 Dec 5.

Faculty of Public Health, Kasetsart University Chalermphrakiat Sakon Nakhon Province Campus, Sakon Nakhon, Thailand. Electronic address:

Random amplification of polymorphic DNA (RAPD) and 16S-23S rDNA intergenic spacer polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) were applied and evaluated to determine clonal complexes (CCs) of 684 Streptococcus suis isolates from pigs and humans. RAPD better distinguished major S. suis CCs than the PCR-RFLP method. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S08908508183018
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http://dx.doi.org/10.1016/j.mcp.2018.12.002DOI Listing
February 2019
5 Reads

Prediction of epitopes of Neisseria Gonorrhoeae against USA human leukocyte antigen background: An immunoinformatic approach towards development of future vaccines for USA population.

Mol Cell Probes 2019 Feb 29;43:40-44. Epub 2018 Nov 29.

Department of Biochemistry and Biotechnology, University of Gujrat, P. O Box No, 50700, Pakistan. Electronic address:

Gonorrheal infections are the second most prevalent sexually transmitted diseases STDs in the USA populations after Chlamydia. These infections have now become an urgent problem to address because Neisseria gonorrhoeae is capable of developing resistance to multiple antibiotic classes. Infection with these antibiotic-resistant strains has become the major public health concern. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S08908508183023
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http://dx.doi.org/10.1016/j.mcp.2018.11.007DOI Listing
February 2019
6 Reads

Selective growth inhibition of Porphyromonas gingivalis and Aggregatibacter actinomycetemcomitans by antisense peptide nucleic acids.

Mol Cell Probes 2019 Feb 22;43:45-49. Epub 2018 Nov 22.

Department of Endodontics, Osaka Dental University, 8-1 Kuzuhahanazonocho, Hirakata, Osaka, 573-1121, Japan.

Peptide nucleic acids (PNA) are DNA/RNA analogs in which the sugar-phosphate backbone is replaced by N-2-aminoethylglycine. PNA are widely used for experimental antisense therapy due to their strong affinity to mRNA. By targeting specific genes, protein synthesis and the growth of bacteria or cancer cells can be inhibited by PNA. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S08908508183026
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http://dx.doi.org/10.1016/j.mcp.2018.11.006DOI Listing
February 2019
7 Reads

Development of a droplet digital PCR assay for sensitive detection of porcine circovirus 3.

Mol Cell Probes 2019 Feb 20;43:50-57. Epub 2018 Nov 20.

Institute of Animal Quarantine, Chinese Academy of Inspection and Quarantine, Beijing, 100176, China. Electronic address:

Porcine circovirus 3 (PCV3), a newly emerged circovirus, is associated with porcine dermatitis and nephropathy syndrome, reproductive failure and multi-systemic inflammation disease, and is widely distributed in pig populations worldwide. Therefore, developing specific diagnostic assays will be important for controlling this emerging pathogen. In this study, we developed a novel droplet digital PCR (ddPCR) assay targeting the PCV3 cap gene to improve the sensitivity of PCV3 detection. Read More

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http://dx.doi.org/10.1016/j.mcp.2018.11.005DOI Listing
February 2019
5 Reads

Development of real-time PCR assays for single and simultaneous detection of infectious bursal disease virus and chicken anemia virus.

Mol Cell Probes 2019 Feb 14;43:58-63. Epub 2018 Nov 14.

Sección Genética Evolutiva, Instituto de Biología, Facultad de Ciencias, Universidad de la República, Iguá 4225, 11400, Montevideo, Uruguay. Electronic address:

Infectious bursal disease virus (IBDV) and chicken anemia virus (CAV) cause relevant immunosuppressive diseases in poultry. Clinical diagnosis of these viruses is challenging given the different disease presentations and the frequent occurrence of co-infections with other pathogens. Here, we standardized and validated simplex and duplex RT-qPCR assays for the straightforward detection of IBDV and CAV. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S08908508183022
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http://dx.doi.org/10.1016/j.mcp.2018.11.004DOI Listing
February 2019
19 Reads

Genes and transcription factors related to the adverse effects of maternal type I diabetes mellitus on fetal development.

Mol Cell Probes 2019 Feb 14;43:64-71. Epub 2018 Nov 14.

Department of Gynecology, Ji'nan Central Hospital Affiliated to Shandong University, Ji'nan, 250013, China.

Purpose: Maternal type I diabetes mellitus (T1DM) increases the risk of adverse pregnancy outcomes, but the corresponding mechanism is unclear. This study aims to investigate the mechanism underlying the adverse pregnancy outcomes of maternal T1DM.

Methods: Gene expression microarray (GSE51546) was down-loaded from the Gene Expression Omnibus. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S08908508183017
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http://dx.doi.org/10.1016/j.mcp.2018.11.003DOI Listing
February 2019
13 Reads

Rapid and sensitive detection of Amphidinium carterae by loop-mediated isothermal amplification combined with a chromatographic lateral-flow dipstick.

Mol Cell Probes 2019 Feb 9;43:72-79. Epub 2018 Nov 9.

School of Marine Science and Technology, Harbin Institute of Technology (Weihai), Weihai, 264209, PR China.

Frequent outbreaks of toxic algal blooms devastate marine ecosystems, marine fisheries, and public health. Monitoring toxic algae is crucial to reduce losses caused by imminent algal blooms. However, traditional detection techniques relying on morphological examination are tedious and time-consuming. Read More

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http://dx.doi.org/10.1016/j.mcp.2018.11.001DOI Listing
February 2019
1 Read

Establishment of rapid detection method and surveillance of budgerigar fledgling disease virus using a TaqMan Real-Time PCR.

Mol Cell Probes 2019 Feb 8;43:80-83. Epub 2018 Nov 8.

Shanghai Key Laboratory of Veterinary Biotechnology, Key Laboratory of Urban Agriculture (South), Ministry of Agriculture, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, 200240, China. Electronic address:

Budgerigar fledgling disease virus (BFDV) infection causes sudden death, abdominal distention, and feather abnormality in psittacine birds. In this study, we developed a TaqMan Real-time PCR assay to detect BFDV by targeting a conserved region in VP1 gene. The detection limit of the assay was 30 DNA gene copies, 1000 times more sensitive than conventional PCR. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S08908508183019
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http://dx.doi.org/10.1016/j.mcp.2018.11.002DOI Listing
February 2019
8 Reads

Reverse transcription recombinase polymerase amplification with lateral flow dipsticks for detection of influenza A virus and subtyping of H1 and H3.

Mol Cell Probes 2018 12 27;42:25-31. Epub 2018 Oct 27.

Ningbo Health BioMed Co., Ltd, Ning Bo, China.

Three reverse transcription recombinase polymerase amplification assays with lateral flow dipsticks (RT-RPA-LFD) were developed for identification of the matrix and hemagglutinin (HA) genes to detect influenza A virus and distinguish subtypes H1 and H3. Assessment of the assays' specificity showed that there was no cross-reactivity with other targets. Their limits of detection were 123. Read More

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http://dx.doi.org/10.1016/j.mcp.2018.10.004DOI Listing
December 2018
2 Reads

Development of double labeling in situ hybridization using RNA probes for genome detection of nervous necrosis virus (NNV).

Mol Cell Probes 2018 12 19;42:18-24. Epub 2018 Oct 19.

Department of Aqualife Medicine, Chonnam National University, Yeosu, Republic of Korea. Electronic address:

In situ hybridization (ISH) of genomic segments using RNA-RNA hybrid for nervous necrosis virus (NNV) detection has not been reported yet. The objective of this study was to develop RNA-ISH using RNA probes for the detection of NNV in infects SSN-1 cells or sevenband grouper Hyporthodus septemfasciatus. Two viral RNA segments viz. Read More

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http://dx.doi.org/10.1016/j.mcp.2018.10.003DOI Listing
December 2018

Bta-miR-130a/b regulates preadipocyte differentiation by targeting PPARG and CYP2U1 in beef cattle.

Mol Cell Probes 2018 12 15;42:10-17. Epub 2018 Oct 15.

College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi, 712100, China; National Beef Cattle Improvement Center, Northwest A&F University, Yangling, Shaanxi, 712100, China. Electronic address:

Deposition of intramuscular fat (IMF) is one of the most important traits for the evaluation of beef carcass quality grade. MicroRNA (miRNA) is an endogenous non-coding RNA that can play a role in the post-transcriptional regulation of mammalian preadipocyte differentiation. Previously, we identified that bta-miR-130a regulates milk fat biosynthesis by targeting PPARG mRNA. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S08908508183021
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http://dx.doi.org/10.1016/j.mcp.2018.10.002DOI Listing
December 2018
2 Reads
1.850 Impact Factor

One-step multiplex real-time RT-PCR for detection and typing of dengue virus.

Mol Cell Probes 2019 Feb 3;43:86-91. Epub 2018 Oct 3.

Kogenebiotech Co., Ltd, Seoul, Republic of Korea. Electronic address:

Previous studies reported that severity of dengue is associated with multiple factors, including secondary infection, age, viral load and infecting serotype and genotype. In addition, other studies have reported that a dengue virus-2 (DENV-2) infection is associated with a prognosis of more severe clinical manifestations than DENV-1 and DENV-4 infections. For these reasons, the ability to identify the DENV serotypes is critical for optimal patient diagnosis and epidemiological studies. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S08908508183012
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http://dx.doi.org/10.1016/j.mcp.2018.10.001DOI Listing
February 2019
17 Reads

In silico investigation of the prion protein glycosylation profiles in relation to scrapie disease resistance in domestic sheep (Ovis aries).

Mol Cell Probes 2018 12 24;42:1-9. Epub 2018 Sep 24.

Ege University, Faculty of Science, Department of Biology, Molecular Biology Section, Izmir, Turkey. Electronic address:

The prion protein is a membrane-bound glycoprotein which consists mainly α-helix structure. In contrast, the infectious prion protein shows the beta-sheet structure. The prion-associated diseases are all lethal neurodegenerative abnormalities, called transmissible spongiform encephalopathies. Read More

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http://dx.doi.org/10.1016/j.mcp.2018.09.004DOI Listing
December 2018

Gold nanoparticle biosensors, a novel application in gene transformation and expression.

Mol Cell Probes 2018 10 7;41:1-7. Epub 2018 Jul 7.

Department of Biology, Faculty of Sciences, University of Zabol, Zabol, Iran.

The conventional techniques of PCR, Southern blot, northern blot, in situ hybridization, and RNase protection assay have long been used to investigate transformation and expression of genes, but most of them are time-consuming and have relatively low sensitivity. In recent years, applying biosensors for molecular identification of biomolecules has been expanding significantly. Hence in this study, Zabol melon was used as a model plant to introduce new DNA and RNA-based biosensors for confirming gene transformation and expression. Read More

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http://dx.doi.org/10.1016/j.mcp.2018.07.002DOI Listing
October 2018

Construction and rescue of Muscovy duck-origin goose parvovirus from an infectious clone containing an E-box deletion within the left terminal region.

Mol Cell Probes 2018 12 18;42:32-35. Epub 2018 Sep 18.

Institute of Animal Husbandry and Veterinary Medicine, Fujian Academy of Agricultural Sciences, Fuzhou, 350013, China; Fujian Animal Diseases Control Technology Development Center, Fuzhou, 350013, China.

To obtain a deletion mutant of Muscovy duck-origin goose parvovirus (MDGPV) and to analyze its biological characteristics, the pMDGPVPT plasmid, which contains a full-length DNA infectious clone of the MDGPV PT strain, was used in this study as the template. The E-box at nt 315 of the left inverted terminal repeat sequence (L-ITR) was deleted by overlap extension PCR to obtain the infectious recombinant plasmid p-PTΔE. The p-PTΔE plasmid was transfected into 9-day-old non-immune Muscovy duck embryos via the yolk sac and the rescued deletion mutant virus r-PTΔE was generated. Read More

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http://dx.doi.org/10.1016/j.mcp.2018.09.003DOI Listing
December 2018

Immunopotentiating effect of Inonotus obliquus fermentation products administered at vaccination in chickens.

Mol Cell Probes 2018 10 15;41:43-51. Epub 2018 Sep 15.

Shandong Key Laboratory of Disease Control and Breeding, Institute of Animal Science and Veterinary Medicine, Shandong Academy of Agricultural Science, No.8, Sangyuan Road, Jinan, Shandong, China. Electronic address:

Vaccination is an important approach for the control of avian viral diseases. The aim of this study was to evaluate the immune-potentiating effect of oral administration of Inonotus obliquus fermentation products (IOFP) at vaccination in chickens. In total, 120 one-day-old specific-pathogen-free chickens were randomly assigned to six groups: groups 1 to 3 were vaccinated with Newcastle disease virus (NDV) LaSota live vaccine via intranasal and eye-dropped route at seven days of age, and boosted two weeks later. Read More

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http://dx.doi.org/10.1016/j.mcp.2018.09.002DOI Listing
October 2018
5 Reads

Expression profile of the CSF3 and LPO genes in milk from buffalo (Bubalus bubalis) with and without mastitis.

Mol Cell Probes 2018 10 12;41:39-42. Epub 2018 Sep 12.

Department of Animal Science, Faculty of Agrarian and Veterinary Sciences, Sao Paulo State University, Via de Acesso Prof. Paulo Donato Castellane s/n, 14884-900, Jaboticabal, SP, Brazil. Electronic address:

This study compared the expression profile of the candidate genes, CSF3 and LPO, by investigating the immune response mechanisms involved in the phenotype of resistance and susceptibility to mastitis of healthy and infected buffaloes. The Granulocyte Colony Stimulating Factor 3 (CSF3) and Lactoperoxidase (LPO) genes expression profiles were determined in 24 milk samples from buffaloes with (N = 12) and without (N = 12) mastitis, using the quantitative real-time PCR (qRT-PCR) technique. CSF3 and LPO expressions were 5. Read More

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http://dx.doi.org/10.1016/j.mcp.2018.09.001DOI Listing
October 2018
1 Read

Development of propidium monoazide-recombinase polymerase amplification (PMA-RPA) assay for rapid detection of Streptococcus pyogenes and Streptococcus agalactiae.

Mol Cell Probes 2018 10 29;41:32-38. Epub 2018 Aug 29.

Shenzhen Academy of Metrology and Quality Inspection/ National Nutrition Food Testing Center (Guangdong), Shenzhen 518000, China. Electronic address:

Streptococcus pyogenes (Group A Streptococcus, GAS) and Streptococcus agalactiae (Group B Streptococcus, GBS) are common pathogens that threaten public health. In this study, a double recombinase polymerase (RPA) amplification assay was developed to rapidly detect these pathogens. Specificity tests revealed that the GAS and GBS strains were positive for speB and SIP genes, respectively. Read More

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http://dx.doi.org/10.1016/j.mcp.2018.08.007DOI Listing
October 2018
6 Reads

Development of a reverse transcription recombinase polymerase amplification assay for rapid detection of Theiler's murine encephalomyelitis virus.

Mol Cell Probes 2018 10 26;41:27-31. Epub 2018 Aug 26.

Guangdong Key Laboratory of Laboratory Animals, Guangdong Laboratory Animals, Monitoring Institute, Guangzhou, China. Electronic address:

Theiler's murine encephalomyelitis virus (TMEV) is one of the most common viral pathogens that circulate widely in captive mouse colonies. A molecular biology detection method would be a useful tool to use in an integrated program to monitor and prevent TMEV infection and transmission. Thus, a reverse transcription recombinase polymerase amplification (RT-RPA) assay was developed to detect TMEV infection. Read More

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http://dx.doi.org/10.1016/j.mcp.2018.08.006DOI Listing
October 2018
19 Reads

Development and validation of a TTR-specific copy number screening tool, and application to potentially relevant patient cohorts.

Mol Cell Probes 2018 10 21;41:61-63. Epub 2018 Aug 21.

Department of Clinical Chemistry and Laboratory Medicine, Jena University Hospital, Am Klinikum 1, 07747, Jena, Germany. Electronic address:

TTR amyloidosis (ATTR) is a fatal condition caused by extracellular deposits of misfolded transthyretin. Patients often present with cardiac disease, but manifestations may also involve other organs including the peripheral nervous system. ATTR is considered familial when heterozygous mutations in the TTR gene are present (ATTRmutant or ATTRm), or acquired when no TTR aberrations are detected (ATTRwildtype or ATTRwt). Read More

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https://linkinghub.elsevier.com/retrieve/pii/S08908508183018
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http://dx.doi.org/10.1016/j.mcp.2018.08.005DOI Listing
October 2018
13 Reads

A lateral flow dipstick combined with reverse transcription recombinase polymerase amplification for rapid and visual detection of the bovine respirovirus 3.

Mol Cell Probes 2018 10 20;41:22-26. Epub 2018 Aug 20.

Key Laboratory of Animal Resistant Biology of Shandong, Ruminant Disease Research Center, College of Life Science, Shandong Normal University, Shandong Province, China. Electronic address:

Bovine respirovirus 3 also known as Bovine parainfluenza virus type 3 (BPIV3) is one of the most important viral respiratory agents of both young and adult cattle. Rapid diagnosis could contribute greatly in containing epidemics and thus avoid economic losses. However, the lack of robust isothermal visual method poses difficulty. Read More

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http://dx.doi.org/10.1016/j.mcp.2018.08.004DOI Listing
October 2018
5 Reads

A real time high-resolution melting PCR assay for detection and differentiation among sheep pox virus, goat pox virus, field and vaccine strains of lumpy skin disease virus.

Mol Cell Probes 2018 10 10;41:57-60. Epub 2018 Aug 10.

Federal Center for Animal Health "FGBI ARRIAH", Vladimir, 600901, Russia. Electronic address:

In this paper, we report on the development of a real time high-resolution melting (HRM) PCR assay for detection and differentiation among sheep pox virus (SPPV), goat pox virus (GTPV), field isolates and vaccine strains of lumpy skin disease virus (LSDV) based on high-resolution melting curve analysis of their target PCR amplicons. A 111 bp region of LSDV010 ORF, which harbors unique genetic differences for each of these viral species, was selected as the PCR target in this study. During the validation of this assay using DNA from clinical isolates originated from naturally infected animals from the different geographic locations and reference strains, the obtained PCR amplicons demonstrated that the melting temperature picks were specific for each tested viral species, i. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S08908508183014
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http://dx.doi.org/10.1016/j.mcp.2018.08.003DOI Listing
October 2018
17 Reads

Validation of high-resolution melting analysis as a diagnostic tool for endothelin receptor B mutation in American Paint horses and allele frequency estimation.

Mol Cell Probes 2018 10 8;41:52-56. Epub 2018 Aug 8.

Department of Veterinary Clinical Science, School of Veterinary Medicine and Animal Science, UNESP - Univ Estadual Paulista, Botucatu, SP, Brazil. Electronic address:

Overo lethal white foal syndrome (OLWFS) is a genetic disorder caused by a dinucleotide mutation in the endothelin receptor type B (EDNRB) gene leading to the death of affected foals shortly after birth. The use of rapid and reliable genetic testing is imperative for the early diagnosis of the mutation avoiding, therefore, either additional suffering or the production of affected animals. In the present study, we developed and validated a high-resolution melting (HRM) genotyping assay to detect the OLWFS causative mutation, and we also determined the frequency of heterozygotes among American Paint horses in Brazil. Read More

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http://dx.doi.org/10.1016/j.mcp.2018.08.002DOI Listing
October 2018

Inhibition of C5a prevents IL-1β-induced alternations in rat synoviocytes in vitro.

Mol Cell Probes 2018 10 6;41:14-21. Epub 2018 Aug 6.

Department of Pain Medicine, Affiliated Hospital of Guizhou Medical University, Guiyang, 550004, Guizhou, PR China.

C5a is an important pro-inflammatory peptide involved in complement activation, membrane attack complex formation, immune cell chemotaxis, and allergic responses. Osteoarthritis is a disease characterized by degenerative changes in articular cartilage. It has recently been found that inflammatory responses play an important role in the pathogenesis of osteoarthritis and also in rheumatoid arthritis, where dysfunctional synoviocytes are involved. Read More

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http://dx.doi.org/10.1016/j.mcp.2018.08.001DOI Listing
October 2018
9 Reads
1.852 Impact Factor

Differential expressions of estrogen and progesterone receptors in endometria and cyst walls of ovarian endometrioma from women with endometriosis and their responses to depo-medroxyprogesterone acetate treatment.

Mol Cell Probes 2018 08 3;40:27-36. Epub 2018 Jul 3.

Department of Anatomy, Faculty of Science, Mahidol University, Bangkok, 10400, Thailand; Faculty of Allied Health Sciences, Burapha University, Chonburi, 20131, Thailand. Electronic address:

Background: Depo-medroxyprogesterone acetate (DMPA) is an injectable progestin contraceptive that provides a highly effective reduction of pelvic pain in women with endometriosis. Despite its wide use to treat pain associated with endometriosis, its precise mechanisms of action remain unclear. The aims of this study were to investigate the differential expressions of estrogen receptors (ERs), and progesterone receptors (PRs) in endometria and ovarian endometrioma cyst walls of women with endometriosis with and without DMPA treatment. Read More

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http://dx.doi.org/10.1016/j.mcp.2018.07.001DOI Listing
August 2018
1 Read

Highly selective and sensitive detection of Staphylococcus aureus with gold nanoparticle-based core-shell nano biosensor.

Mol Cell Probes 2018 10 25;41:8-13. Epub 2018 Jul 25.

Biology Research Center, Zanjan Branch, Islamic Azad University, Zanjan, Iran.

Staphylococcus aureus is a gram-positive and opportunistic pathogen that is one of the most common causes of nosocomial infections; therefore, its rapid diagnosis is important and valuable. Today, the use of nanoparticles is expanding due to their unique properties. The purpose of the present study is the determination of S. Read More

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http://dx.doi.org/10.1016/j.mcp.2018.07.004DOI Listing
October 2018
12 Reads
1.852 Impact Factor

MiR-1297 suppresses pancreatic cancer cell proliferation and metastasis by targeting MTDH.

Mol Cell Probes 2018 08 13;40:19-26. Epub 2018 Jun 13.

Department of Hepatobiliary Surgery, Affiliated Hospital of Guizhou Medical University, 28 Guiyi Street, Yunyan District, Guiyang, 550004, Guizhou, China. Electronic address:

Dysregulation of miR-1297 has been detected in various human cancers, and miR-1297 can function as either an oncogene or tumor suppressor. However, the role of miR-1297 in pancreatic adenocarcinoma has not been previously reported. Here, we investigated miR-1297 expression in pancreatic cancer and the role it plays in the development and metastasis of pancreatic adenocarcinoma. Read More

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http://dx.doi.org/10.1016/j.mcp.2018.06.003DOI Listing
August 2018
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Design and evaluation of nested PCR primers for specific detection of genogroup I noroviruses in oysters.

Mol Cell Probes 2018 08 11;40:40-43. Epub 2018 Jun 11.

College of Food Science and Technology, Shanghai Ocean University, Shanghai, China; Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao, China; Laboratory of Quality and Safety Risk Assessment for Aquatic Products on Storage and Preservation (Shanghai), Ministry of Agriculture, China. Electronic address:

A pair of nested PCR universal primers (NGIOF and NGIOR) specific for genogroup I (GI) noroviruses was designed based on all GI sequences available in public databases. The primers were evaluated for their specificity, sensitivity and coverage, which demonstrate their reliable performance upon detection of GI noroviruses in oysters. Read More

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http://dx.doi.org/10.1016/j.mcp.2018.06.002DOI Listing
August 2018
7 Reads

Duplex real-time polymerase chain reaction assay for the detection of human KIPyV and WUPyV in nasopharyngeal aspirate pediatric samples.

Mol Cell Probes 2018 08 5;40:13-18. Epub 2018 Jun 5.

Microbiology and Virology Unit, Department of Diagnostics and Public Health, University of Verona, Strada delle Grazie 8, 37134 Verona, Italy.

In this study, we describe a duplex real-time PCR assay for the simultaneous detection of KIPyV and WUPyV polyomaviruses based on TaqMan probes. This assay detected 500 copies/mL both for KIPyV and WUPyV in 100% of tested positive samples. We assessed this technique on 482 nasopharyngeal aspirate specimens from hospitalized pediatric patients with respiratory symptoms, previously analyzed with commercial multiplex assay for 16 major respiratory viruses. Read More

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http://dx.doi.org/10.1016/j.mcp.2018.06.001DOI Listing
August 2018
9 Reads

Identification of bta-miR-15a∼16a cluster expression, localization and regulated target in Holsteins.

Mol Cell Probes 2018 08 30;40:8-12. Epub 2018 May 30.

Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education in China, Huazhong Agricultural University, Wuhan, 430070, Hubei, China. Electronic address:

Bovine mastitis is an inflammation response of the mammary gland tissues caused mainly by pathogenic bacteria in cows. Previous studies showed that bta-miR-15a and bta-miR-16a modulate immunity and inflammation responses. In this study, we investigated the expression pattern and tissue localization of bta-miR-15a and bta-miR-16a. Read More

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http://dx.doi.org/10.1016/j.mcp.2018.05.003DOI Listing
August 2018
2 Reads

The development and application of a duplex reverse transcription loop-mediated isothermal amplification assay combined with a lateral flow dipstick method for Macrobrachium rosenbergii nodavirus and extra small virus isolated in China.

Mol Cell Probes 2018 08 22;40:1-7. Epub 2018 May 22.

School of Food and Biological Engineering, Jiangsu University, Zhenjiang, 212013, China; Institute of Life Sciences, Jiangsu University, Zhenjiang, 212013, China. Electronic address:

White tail disease (WTD), a major disease prevailing in the larval stage of Macrobrachium rosenbergii, caused by Macrobrachium rosenbergii nodavirus (MrNV) associated with extra small virus (XSV), led to the economic loss of shrimp industry in China. In order to establish a convenient, sensitive and selective molecular diagnostic method to detect MrNV and XSV for the Chinese shrimp (MrNV/XSV-chin), a reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay combined with a lateral flow dipstick (LFD) method were developed. A set of four specific primers and a labeled probe were designed according to the six conserved gene sequence regions encoding for the MrNV capsid protein CP43 and the XSV capsid protein CP17. Read More

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http://dx.doi.org/10.1016/j.mcp.2018.05.001DOI Listing
August 2018
6 Reads

Flow cytometry-based method facilitates optimization of PMA treatment condition for PMA-qPCR method.

Mol Cell Probes 2018 08 22;40:37-39. Epub 2018 May 22.

Fujian Medical University Union Hospital, Fuzhou, Fujian Province 350001, China; Fujian Medical University Stem Cell Research Institute, Fuzhou, Fujian Province 350004, China. Electronic address:

Coupling propidium monoazide (PMA) with quantitative PCR (PMA-qPCR) has been successfully applied to specific detection and quantification of viable cells in various samples. The optimal PMA treatment condition is usually determined through qPCR. However, it is a tedious, time consuming and costly process including DNA extraction and qPCR. Read More

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http://dx.doi.org/10.1016/j.mcp.2018.05.002DOI Listing
August 2018
1 Read
1.852 Impact Factor

Equipment-free recombinase polymerase amplification assay using body heat for visual and rapid point-of-need detection of canine parvovirus 2.

Mol Cell Probes 2018 06 27;39:41-46. Epub 2018 Apr 27.

College of Veterinary Medicine, Agricultural University of Hebei, Baoding 071001, China. Electronic address:

A visible and equipment-free recombinase polymerase amplification assay combined with a lateral flow strip (LFS RPA) was developed to detect canine parvovirus type 2 (CPV-2), which is the etiological agent of canine parvovirus disease. The CPV-2 LFS RPA assay was developed based on the VP2 gene and is performed in a closed fist using body heat for 15 min; the products are visible to the naked eye on the LFS within 5 min. The assay could detect CPV-2a, CPV-2b and CPV-2c, and there was no cross-reaction with the other viruses tested. Read More

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http://dx.doi.org/10.1016/j.mcp.2018.04.004DOI Listing
June 2018
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Rapid detection of goose hemorrhagic polyomavirus using TaqMan quantitative real-time PCR.

Mol Cell Probes 2018 06 14;39:61-64. Epub 2018 Apr 14.

Fujian Provincial Key Laboratory for Avian Diseases Control and Prevention/Fujian Animal Diseases Control Technology Development Center, Institute of Animal Husbandry and Veterinary Medicine of Fujian Academy of Agricultural Sciences, Fuzhou 350013, People's Republic of China. Electronic address:

Due to low doses of infection, an efficient and sensitive virus detection method is necessary to detect low amounts of goose hemorrhagic polyomavirus (GHPV). In this study, we have developed a TaqMan real-time PCR (qPCR) specific assay for the detection of GHPV. Specificity assay showed no cross-reactions with other common waterfowl viruses. Read More

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http://dx.doi.org/10.1016/j.mcp.2018.04.003DOI Listing
June 2018
6 Reads

The establishment of methods for free PAR generation and PAR reader detection.

Mol Cell Probes 2018 06 5;39:57-60. Epub 2018 Apr 5.

The Key Laboratory of Molecular Epigenetics of the Ministry of Education, Northeast Normal University, Changchun, Jilin 130024, China; School of Life Science, Northeast Normal University, Changchun, Jilin 130024, China. Electronic address:

Poly (ADP-ribose) polymerase 1 (PARP1) is a DNA damage sensor that catalyzes the poly (ADP-ribose) (PAR) onto a variety of target proteins, such as histones, DSB repair factors and PARP1 itself under consumption of NAD. Besides, PARP1 can affect a variety of proteins in noncovalent modification manner to carry out specific cellular functions. Here, we established a method to generate non-radiolabeled free PAR by PARG moderately cleaving PAR from autoPARylated PARP1, and utilized dot-blot assay to determine the interaction between free PAR and interested proteins. Read More

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http://dx.doi.org/10.1016/j.mcp.2018.04.002DOI Listing
June 2018
10 Reads