1,869 results match your criteria Molecular and Cellular Probes [Journal]


A TaqMan probe-based real-time PCR to differentiate porcine epidemic diarrhea virus virulent strains from attenuated vaccine strains.

Mol Cell Probes 2019 Apr 17. Epub 2019 Apr 17.

College of Animal Medicine, Hebei Agricultural University, Baoding, Hebei, 071000, China. Electronic address:

Porcine epidemic diarrhea virus (PEDV) is an important pathogen causing severe watery diarrhea, vomiting, dehydration, and death in sucking piglets. Attenuated vaccines have been used widely in sows in order to protect piglets through passive lactogenic immunity. Rapid and sensitive detection methods for differentiating attenuated vaccine strains from virulent ones are essential and practical in PEDV prevention and control. Read More

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http://dx.doi.org/10.1016/j.mcp.2019.04.003DOI Listing

An exonuclease-assisted fluorescence sensor for assaying alkaline phosphatase based on SYBR Green I.

Mol Cell Probes 2019 Apr 12. Epub 2019 Apr 12.

School of Life Sciences, Central South University, Changsha, 410013, China.

In this report, we propose a fast, reliable and convenient approach to determine the alkaline phosphatase (ALP) activity based on a label-free fluorescence strategy. Upon catalysis of ALP, dephosphorylated dsDNA hampers the λ exonuclease (λexo) cleavage, shows high affinity to SYBR Green I (SG I), resulting in a strong fluorescence emission peak at 520 nm. In the absence of ALP, the dsDNA with 5'-phosphoryl-termini could be employed as a substrate of λexo. Read More

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http://dx.doi.org/10.1016/j.mcp.2019.04.002DOI Listing

Development of a TB green II-based duplex real-time fluorescence quantitative PCR assay for the simultaneous detection of porcine circovirus 2 and 3.

Mol Cell Probes 2019 Apr 10. Epub 2019 Apr 10.

Zhengzhou Key Laboratory for Pig Disease Prevention and Control, College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengdong New District Longzi Lake 15#, Zhengzhou 450046, Henan Province, People's Republic of China.

Porcine circovirus 3 (PCV3), as a newly emerged circovirus, is widely distributed in pig populations worldwide. Co-infection of PCV2 and PCV3 has been reported frequently in clinical samples. In the present study, a TB Green II-based duplex real-time polymerase chain reaction (qPCR) was developed to rapidly and differentially detect PCV2 and PCV3. Read More

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http://dx.doi.org/10.1016/j.mcp.2019.04.001DOI Listing

Identification of FimH derivatives as adjuvant vaccinated with PAc that enhance protection against Streptcoccus mutans colonization.

Mol Cell Probes 2019 Mar 30. Epub 2019 Mar 30.

The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST), Key Laboratory of Oral Biomedicine of Ministry of Education (KLOBM), School and Hospital of Stomatology, Wuhan University, Wuhan, 430079, China. Electronic address:

FimH is the adhesin of type I fimbriae expressed on Escherichia coli that can mediate specific adherence to host cells. High binding mutations in FimH are related to the adaptive evolution of bacteria. However, additional roles that these allelic variations may play remain elusive. Read More

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http://dx.doi.org/10.1016/j.mcp.2019.03.009DOI Listing

Fabry disease: Detection of Alu-mediated exon duplication by NGS.

Mol Cell Probes 2019 Mar 29. Epub 2019 Mar 29.

Clinic for General and Interventional Cardiology/Angiology, Herz- und Diabeteszentrum NRW, Ruhr-Universität Bochum, Bad Oeynhausen, Germany.

Monogenetic diseases can be analyzed routinely by targeted DNA sequencing. If causative variants are not found, complementary methods like RNA sequencing or analysis of copy number variations by multiplex ligation-dependent probe amplification have to be considered. In the latter, especially exonic duplications or deletions can be detected, but the precise sites of mutations remain unclear. Read More

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http://dx.doi.org/10.1016/j.mcp.2019.03.008DOI Listing
March 2019
2 Reads

Direct detection of Actinobacillus pleuropneumoniae in swine lungs and tonsils by real-time recombinase polymerase amplification assay.

Mol Cell Probes 2019 Mar 28. Epub 2019 Mar 28.

College of Veterinary Medicine, Hebei Agricultural University, Baoding, 071001, China. Electronic address:

Actinobacillus pleuropneumoniae is the etiological agent of swine contagious pleuropneumoniae, which is distributed globally and associated with severe economic losses in the pig rearing industry. In this study, a real-time recombinase polymerase amplification assay (real-time RPA) based on the apxIVA gene was developed to rapid detect A. pleuropneumoniae. Read More

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http://dx.doi.org/10.1016/j.mcp.2019.03.007DOI Listing

Corrigendum to "Enhanced multiplex PCR assay for the typing of staphylococcal cassette chromosome mec types I to V in methicillin-resistant Staphylococcus aureus".

Mol Cell Probes 2019 Mar 27. Epub 2019 Mar 27.

Centre for Antimicrobial Resistance, Alberta Health Services, Calgary Laboratory Services, University of Calgary, Calgary, Alberta, Canada; Department of Pathology & Laboratory Medicine, University of Calgary, Calgary, Alberta, Canada; Department of Microbiology, Immunology and Infectious Diseases, University of Calgary, Calgary, Alberta, Canada; Department of Medicine, University of Calgary, Calgary, Alberta, Canada; The Calvin, Phoebe and Joan Snyder Institute for Chronic Diseases, University of Calgary, Calgary, Alberta, Canada; Calgary Laboratory Services, Calgary, Alberta, Canada.

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http://dx.doi.org/10.1016/j.mcp.2019.03.004DOI Listing
March 2019
1 Read

Development of a reverse transcription recombinase polymerase amplification assay for rapid detection of human respiratory syncytial virus.

Mol Cell Probes 2019 Mar 25. Epub 2019 Mar 25.

Department of Clinical Laboratory, The Third Affiliated Hospital of Southern Medical University, Guangzhou, China.

Respiratory syncytial virus (RSV) is one of the most important causative agents that causing respiratory tract infection in children and associated with high morbidity and mortality. A diagnostic method would be a robust tool for identification of RSV infection, especially in the resource-limited settings. Recombinase polymerase amplification (RPA) is a novel isothermal amplification technique which has been widely employed to detect human/animal pathogens. Read More

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http://dx.doi.org/10.1016/j.mcp.2019.03.005DOI Listing

Which genes to assess in the NGS diagnostics of intellectual disability? The case for a consensus database-driven and expert-curated approach.

Mol Cell Probes 2019 Mar 23. Epub 2019 Mar 23.

Institute of Human Genetics, University Hospital Cologne, Kerpener Str. 34, 50931, Cologne, Germany.

When deciding on which genes to assess in larger Next-Generation Sequencing (NGS) datasets for the molecular genetic diagnosis of intellectual disability (ID), geneticists today have a variety of gene-phenotype databases and expert-curated gene lists available. To quantify their respective completeness, we compare an ID gene selection auto-generated from the Human Phenotype Ontology gene-phenotype association database and expert-curated ID gene lists from three reputable sources (sysID, the DDD consortium and Genomics England) and analyse some of their differences. We give examples of what we regard as genuine gaps ("missing ID genes") for each of these and conclude that a complementary or consensus approach is needed to maximise diagnostic yield in ID patients. Read More

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http://dx.doi.org/10.1016/j.mcp.2019.03.006DOI Listing
March 2019
1 Read

Α 2-stage, nested-like nucleic acid amplification method (IsoPCR) for the highly sensitive detection of HPV16 and HPV18 DNA.

Mol Cell Probes 2019 Mar 19. Epub 2019 Mar 19.

University of Thessaly, School of Health Sciences, Department of Biochemistry & Biotechnology, Microbiology-Virology Laboratory, Biopolis, 41500, Larissa, Greece.

Molecular detection of HPV DNA is considered as the gold standard for the diagnosis of cervical disease. Although the molecular assays for the identification of HPV16 and HPV18 have helped identify cervical cancer incidents, they are restricted to specialized laboratories. Thus, we developed a novel 2-stage, nested-like nucleic acid amplification method, named IsoPCR, to amplify the E6 gene of HPV16 and HPV18 with high analytical sensitivity and specificity. Read More

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http://dx.doi.org/10.1016/j.mcp.2019.03.003DOI Listing

Use of whole exome sequencing in the NICU: Case of an extremely low birth weight infant with syndromic features.

Mol Cell Probes 2019 Mar 15. Epub 2019 Mar 15.

University of Cologne, Medical Faculty and University Hospital, Department of Pediatrics, Division of Pediatric Neurology, Germany; University of Cologne, Medical Faculty, Center for Molecular Medicine Cologne (CMMC), Germany. Electronic address:

Single gene (Mendelian) disorders are one of the leading causes of neonatal morbidity and mortality. However, in the setting of preterm birth phenotypic features of genetic diseases are often undifferentiated and are clinically very difficult to interpret based on the wide range of differential diagnoses. We report an extremely low birth weight infant (ELBW) born prematurely at 23 + 0 gestational weeks after twin pregnancy with a novel clinical manifestation with persistent hyperglycaemia as well as the known manifestations of disease-associated hypokinesia, renal salt wasting, and multifocal atrial tachycardia. Read More

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http://dx.doi.org/10.1016/j.mcp.2019.03.002DOI Listing
March 2019
2 Reads

Probe-specific loop-mediated isothermal amplification magnetogenosensor assay for rapid and specific detection of pathogenic Leptospira.

Mol Cell Probes 2019 04 12;44:63-68. Epub 2019 Mar 12.

Department of Medical Microbiology & Parasitology, School of Medical Sciences, Health Campus, Universiti Sains Malaysia, 16150, Kota Bharu, Kelantan, Malaysia. Electronic address:

This study highlighted the performance of the developed integrated loop-mediated isothermal amplification (LAMP) coupled with a colorimetric DNA-based magnetogenosensor. The biosensor operates through a DNA hybridization system in which a specific designed probe captures the target LAMP amplicons. We demonstrated the magnetogenosensor assay by detecting pathogenic Leptospira, which causes leptospirosis. Read More

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http://dx.doi.org/10.1016/j.mcp.2019.03.001DOI Listing

CDKL1 promotes the chemoresistance of human oral squamous cell carcinoma cells to hydroxycamptothecin.

Mol Cell Probes 2019 04 22;44:57-62. Epub 2019 Feb 22.

Department of Human Anatomy, Histology and Embryology, Shandong University School of Medicine, Jinan, 250000, PR China. Electronic address:

CDKL1 is a cyclin-dependent kinase-like kinase that is highly expressed in diverse types of cancer cells. However, the role of CDKL1 in the chemoresistance of oral squamous cell carcinoma (OSCC) remains largely undefined. Here, we explored the role of CDKL1 in the chemoresistance of the human OSCC cell line CAL27 to hydroxycamptothecin (HCPT). Read More

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http://dx.doi.org/10.1016/j.mcp.2019.02.004DOI Listing
April 2019
3 Reads

EvaGreen-based real-time PCR assay for sensitive detection of enzootic nasal tumor virus 2.

Mol Cell Probes 2019 04 13;44:51-56. Epub 2019 Feb 13.

College of Animal Science and Technology, Southwest University, Chongqing, 400715, China. Electronic address:

Enzootic nasal tumor virus 2 (ENTV-2), the aetiological agent of enzootic nasal adenocarcinoma in goats, is prevalent in China; resulting in substantial economic losses to the goat-breeding industry. Therefore, it is necessary to establish an efficient detection method for the diagnosis and prevention of ENTV-2 infection. More recently, EvaGreen is emerging as a novel alternative fluorescent dye for quantitative real-time PCR because of its low cost, specific amplification and high resolution. Read More

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http://dx.doi.org/10.1016/j.mcp.2019.02.003DOI Listing

Development of a SYBR green I-based duplex real-time fluorescence quantitative PCR assay for the simultaneous detection of porcine epidemic diarrhea virus and porcine circovirus 3.

Mol Cell Probes 2019 04 5;44:44-50. Epub 2019 Feb 5.

College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengdong New District Longzi Lake 15#, Zhengzhou, 450046, Henan Province, People's Republic of China; Zhengzhou Major Pig Disease Prevention and Control Laboratory, Zhengzhou, 450046, Henan Province, People's Republic of China. Electronic address:

The development of a rapid, specific, and sensitive SYBR Green I-based duplex real-time quantitative PCR assay is described for the simultaneous detection of porcine epidemic diarrhea virus (PEDV) and porcine circovirus type 3 (PCV3). The assay specifically detected PEDV and PCV3, with no fluorescence detected for other non-targeted pig pathogens. The assay showed a good linear relationship, and the limits of detection for this assay were 34. Read More

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http://dx.doi.org/10.1016/j.mcp.2019.02.002DOI Listing
April 2019
3 Reads

MiR-486-5p inhibits the proliferation of leukemia cells and induces apoptosis through targeting FOXO1.

Mol Cell Probes 2019 04 5;44:37-43. Epub 2019 Feb 5.

Department of Hematology, The First Affiliated Hospital of Xi'an Medical University, China. Electronic address:

Aim: Studies have reported that micro (miR)-486-5p plays a crucial part in the progression of leukemia, however, to the best of our knowledge, few studies have been conducted on its mechanism in leukemia. In this study, the mechanism of miR-486-5p in leukemia cells was pointed out and its possible target genes were analyzed for the purpose of providing new therapeutic strategies for treating leukemia patients.

Methods: MiRNA expression of Leukemia cells (K562, Kasumi-1, and THP-1) and primary leukocytes was detected by Real-time Quantitative polymerase chain reaction(qPCR). Read More

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https://linkinghub.elsevier.com/retrieve/pii/S08908508193000
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http://dx.doi.org/10.1016/j.mcp.2019.02.001DOI Listing
April 2019
8 Reads

MicroRNA-224 impairs adipogenic differentiation of bovine preadipocytes by targeting LPL.

Mol Cell Probes 2019 04 28;44:29-36. Epub 2019 Jan 28.

College of Animal Science and Technology, National Beef Cattle Improvement Center, Northwest A&F University, Yangling, Shaanxi, China. Electronic address:

In this study, the potential functions of miR-224 in regulating adipogenic differentiation were explored in bovine preadipocytes. Comparative transcriptome analysis between castrated male cattle with increased intramuscular fat (IMF) and intact male cattle revealed that miR-224 and LPL were abnormally expressed, correlating negatively, and LPL was a predicted target of miR-224. A dual luciferase reporter assay confirmed the negative targeting regulatory relationship between miR-224 and LPL. Read More

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http://dx.doi.org/10.1016/j.mcp.2019.01.005DOI Listing

Downregulated low-density lipoprotein receptor-related protein 6 induces the maldevelopment of extravillous trophoblast via Wnt/β-catenin signaling pathway.

Mol Cell Probes 2019 04 24;44:21-28. Epub 2019 Jan 24.

Department of Obstetrics, The Affiliated Yantai Yuhuangding Hospital of Qingdao University, China. Electronic address:

Preeclampsia (PE), a special type of hypertensive disorder complicating pregnancy (HDCP), is highly associated with the migratory and invasive capacity of extravillous trophoblasts (EVTs). Here, we aimed to study the functions of low-density lipoprotein receptor-related protein 6 (LRP6) in PE pathogenesis. A comparative analysis of cellular gene expressions between placenta tissues collected from PE patients and normal pregnant women showed that the expressions of LRP6, β-catenin and matrix metallopeptidases/TIMP metallopeptidase inhibitors (MMPs/TIMPs) ratio in placentas of PE patients were much lower than the normal. Read More

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http://dx.doi.org/10.1016/j.mcp.2019.01.004DOI Listing
April 2019
2 Reads

HADHA and HADHB gene associated phenotypes - Identification of rare variants in a patient cohort by Next Generation Sequencing.

Mol Cell Probes 2019 04 22;44:14-20. Epub 2019 Jan 22.

Medical Genetics Center, Munich, Germany; Department of Neurology, Friedrich-Baur-Institute, Klinikum der Ludwig-Maximilians-University, Munich, Germany. Electronic address:

The heterooctameric mitochondrial trifunctional protein (MTP), composed of four α- and β-subunits harbours three enzymes that each perform a different function in mitochondrial fatty acid β-oxidation. Pathogenic variants in the MTP genes (HADHA and HADHB) cause MTP deficiency, a rare autosomal recessive metabolic disorder characterized by phenotypic heterogeneity ranging from severe, early-onset, cardiac disease to milder, later-onset, myopathy and neuropathy. Since metabolic myopathies and neuropathies are a group of rare genetic disorders and their associated muscle symptoms may be subtle, the diagnosis is often delayed. Read More

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http://dx.doi.org/10.1016/j.mcp.2019.01.003DOI Listing
April 2019
2 Reads

Novel dual-color drug screening model for GLUT4 translocation in adipocytes.

Mol Cell Probes 2019 02 11;43:6-12. Epub 2019 Jan 11.

State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, 650201, China; The General Hospital of Ningxia Medical University, Department of Beijing National Biochip Research Center Sub-Center in Ningxia, Yinchuan, 750004, China. Electronic address:

Insulin-responsive glucose transporter type 4 (GLUT4) translocation plays a major role in controlling glucose uptake in adipose tissue and muscle, maintaining homeostasis and preventing hyperglycemia. Screening for chemicals enhancing GLUT4 translocation is an approach for identifying hits of drug development for type 2 diabetes. Here we developed a novel functional dual-color probe, pHluorin-GLUT4-mOrange2, and constructed 3T3-L1 adipocytes based screening system to simply and efficiently screen new compounds stimulating GLUT4 translocation. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S08908508183027
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http://dx.doi.org/10.1016/j.mcp.2019.01.002DOI Listing
February 2019
13 Reads

Next generation sequencing and imprinting disorders: Current applications and future perspectives: Lessons from Silver-Russell syndrome.

Mol Cell Probes 2019 04 2;44:1-7. Epub 2019 Jan 2.

Institute of Human Genetics, University Hospital, Technical University RWTH, Aachen, Germany. Electronic address:

Imprinting Disorders are a group of rare diseases with overlapping phenotypes which are associated with similar molecular changes and affect imprinted chromosomal regions. Clinical features mainly occur prenatally or in childhood, but have a severe lifelong impact on health. Due to their clinical and molecular heterogeneity, the diagnosis of imprinting disorders is often challenging and requires testing of a broad spectrum of genomic variants and aberrant methylation of imprinted loci (epimutations). Read More

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http://dx.doi.org/10.1016/j.mcp.2018.12.007DOI Listing

A real-time recombinase polymerase amplification assay for the rapid detection of Vibrio harveyi.

Mol Cell Probes 2019 04 3;44:8-13. Epub 2019 Jan 3.

Ningbo Institute of Oceanography, Ningbo, 315832, China.

Vibrio harveyi is a pathogen that infects fish and shellfish worldwide, causing severe economic losses for the aquaculture industry. As the early diagnosis of V. harveyi infection is crucial to disease surveillance and prevention in cultured marine animals, a fast and accurate method to detect V. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S08908508183027
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http://dx.doi.org/10.1016/j.mcp.2019.01.001DOI Listing
April 2019
6 Reads

Direct comparison of QIAamp DSP Virus Kit and QIAamp Circulating Nucleic Acid Kit regarding cell-free fetal DNA isolation from maternal peripheral blood.

Mol Cell Probes 2019 02 22;43:13-19. Epub 2018 Dec 22.

Medical Research and Education Center, Lomonosov Moscow State University, Lomonosovsky Prospect, 27/10, 119192, Moscow, Russia. Electronic address:

Background: Blood of pregnant women contains cell-free fetal DNA (cffDNA), which is widely used in non-invasive prenatal diagnosis. The modern laboratory equipment market provides huge variety of commercial kits for isolation of circulating nucleic acids, but unfortunately none of them are standardized for isolation of cffDNA, which is a crucial step for success of subsequent analysis.

Aim: To compare DSPVK and CNAK in terms of cffDNA, cell-free total DNA (cftDNA) yield and resulting cffDNA fraction, as well as to try to explain the possible difference between the efficacy of these kits. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S08908508183025
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http://dx.doi.org/10.1016/j.mcp.2018.12.006DOI Listing
February 2019
22 Reads

Rapid detection of Cucumber green mottle mosaic virus by reverse transcription recombinase polymerase amplification.

Mol Cell Probes 2019 02 22;43:84-85. Epub 2018 Dec 22.

Institute of Eco-Environment and Plant Protection, Shanghai Key Laboratory of Protection Horticultural Technology, Shanghai Academy of Agricultural Sciences, Shanghai, 201403, China. Electronic address:

Reverse transcription recombinase polymerase amplification (RT-RPA) for detection of Cucumber green mottle mosaic virus (CGMMV) was developed in this study. It was found to be specific, with a limit of detection of 0.5 pg of total CGMMV RNA. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S08908508183030
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http://dx.doi.org/10.1016/j.mcp.2018.12.005DOI Listing
February 2019
8 Reads

Multiplex SYBR Green and duplex TaqMan real-time PCR assays for the detection of Photorhabdus Insect-Related (Pir) toxin genes pirA and pirB.

Mol Cell Probes 2019 02 18;43:20-28. Epub 2018 Dec 18.

Aquaculture Pathology Laboratory, School of Animal and Comparative Biomedical Sciences, The University of Arizona, AZ. 85721, USA. Electronic address:

Acute hepatopancreatic necrosis disease (AHPND), also known as Early mortality syndrome (EMS), is a recently emerged lethal disease that has caused major economic losses in shrimp aquaculture. The etiologic agents are Vibrio spp. that carry Photorhabdus Insect-Related (Pir) toxin genes pirA and pirB. Read More

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http://dx.doi.org/10.1016/j.mcp.2018.12.004DOI Listing
February 2019

A novel fluorometric method for inorganic pyrophosphatase detection based on G-quadruplex-thioflavin T.

Mol Cell Probes 2019 02 17;43:29-33. Epub 2018 Dec 17.

School of Life Sciences, Central South University, Changsha, 410013, China.

In this paper, we propose a fluorometric approach for the highly sensitive detection of inorganic pyrophosphatase (PPase) based on G-quadruplex-thioflavin T (ThT). In the absence of PPase, Cu can coordinate with pyrophosphate (PPi) to generate a Cu/PPi complex. Then the G-rich sequence folds into the G-quadruplex structure, which can combine with ThT to generate a remarkable fluorescent signal. Read More

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http://dx.doi.org/10.1016/j.mcp.2018.12.003DOI Listing
February 2019

Ginsenoside Rb1 and mitochondria: A short review of the literature.

Mol Cell Probes 2019 02 4;43:1-5. Epub 2018 Dec 4.

Institute of Medicinal Plant Development, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing, 100193, China. Electronic address:

Mitochondria play a central role in various critical cellular processes, including energy synthesis, energy supply and apoptosis. Panax notoginseng, a commonly used traditional Chinese medicine, has various pharmacological effects on the human body. Ginsenosides are representative bioactive components of P. Read More

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http://dx.doi.org/10.1016/j.mcp.2018.12.001DOI Listing
February 2019
1 Read

Application of random amplified polymorphism DNA and 16S-23S rDNA intergenic spacer polymerase chain reaction-restriction fragment length polymorphism to predict major Streptococcus suis clonal complexes isolated from humans and pigs.

Mol Cell Probes 2019 02 5;43:34-39. Epub 2018 Dec 5.

Faculty of Public Health, Kasetsart University Chalermphrakiat Sakon Nakhon Province Campus, Sakon Nakhon, Thailand. Electronic address:

Random amplification of polymorphic DNA (RAPD) and 16S-23S rDNA intergenic spacer polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) were applied and evaluated to determine clonal complexes (CCs) of 684 Streptococcus suis isolates from pigs and humans. RAPD better distinguished major S. suis CCs than the PCR-RFLP method. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S08908508183018
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http://dx.doi.org/10.1016/j.mcp.2018.12.002DOI Listing
February 2019
6 Reads

Prediction of epitopes of Neisseria Gonorrhoeae against USA human leukocyte antigen background: An immunoinformatic approach towards development of future vaccines for USA population.

Mol Cell Probes 2019 02 29;43:40-44. Epub 2018 Nov 29.

Department of Biochemistry and Biotechnology, University of Gujrat, P. O Box No, 50700, Pakistan. Electronic address:

Gonorrheal infections are the second most prevalent sexually transmitted diseases STDs in the USA populations after Chlamydia. These infections have now become an urgent problem to address because Neisseria gonorrhoeae is capable of developing resistance to multiple antibiotic classes. Infection with these antibiotic-resistant strains has become the major public health concern. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S08908508183023
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http://dx.doi.org/10.1016/j.mcp.2018.11.007DOI Listing
February 2019
11 Reads

Selective growth inhibition of Porphyromonas gingivalis and Aggregatibacter actinomycetemcomitans by antisense peptide nucleic acids.

Mol Cell Probes 2019 02 22;43:45-49. Epub 2018 Nov 22.

Department of Endodontics, Osaka Dental University, 8-1 Kuzuhahanazonocho, Hirakata, Osaka, 573-1121, Japan.

Peptide nucleic acids (PNA) are DNA/RNA analogs in which the sugar-phosphate backbone is replaced by N-2-aminoethylglycine. PNA are widely used for experimental antisense therapy due to their strong affinity to mRNA. By targeting specific genes, protein synthesis and the growth of bacteria or cancer cells can be inhibited by PNA. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S08908508183026
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http://dx.doi.org/10.1016/j.mcp.2018.11.006DOI Listing
February 2019
13 Reads

Development of a droplet digital PCR assay for sensitive detection of porcine circovirus 3.

Mol Cell Probes 2019 02 20;43:50-57. Epub 2018 Nov 20.

Institute of Animal Quarantine, Chinese Academy of Inspection and Quarantine, Beijing, 100176, China. Electronic address:

Porcine circovirus 3 (PCV3), a newly emerged circovirus, is associated with porcine dermatitis and nephropathy syndrome, reproductive failure and multi-systemic inflammation disease, and is widely distributed in pig populations worldwide. Therefore, developing specific diagnostic assays will be important for controlling this emerging pathogen. In this study, we developed a novel droplet digital PCR (ddPCR) assay targeting the PCV3 cap gene to improve the sensitivity of PCV3 detection. Read More

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http://dx.doi.org/10.1016/j.mcp.2018.11.005DOI Listing
February 2019
11 Reads

Development of real-time PCR assays for single and simultaneous detection of infectious bursal disease virus and chicken anemia virus.

Mol Cell Probes 2019 02 14;43:58-63. Epub 2018 Nov 14.

Sección Genética Evolutiva, Instituto de Biología, Facultad de Ciencias, Universidad de la República, Iguá 4225, 11400, Montevideo, Uruguay. Electronic address:

Infectious bursal disease virus (IBDV) and chicken anemia virus (CAV) cause relevant immunosuppressive diseases in poultry. Clinical diagnosis of these viruses is challenging given the different disease presentations and the frequent occurrence of co-infections with other pathogens. Here, we standardized and validated simplex and duplex RT-qPCR assays for the straightforward detection of IBDV and CAV. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S08908508183022
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http://dx.doi.org/10.1016/j.mcp.2018.11.004DOI Listing
February 2019
32 Reads

Genes and transcription factors related to the adverse effects of maternal type I diabetes mellitus on fetal development.

Mol Cell Probes 2019 02 14;43:64-71. Epub 2018 Nov 14.

Department of Gynecology, Ji'nan Central Hospital Affiliated to Shandong University, Ji'nan, 250013, China.

Purpose: Maternal type I diabetes mellitus (T1DM) increases the risk of adverse pregnancy outcomes, but the corresponding mechanism is unclear. This study aims to investigate the mechanism underlying the adverse pregnancy outcomes of maternal T1DM.

Methods: Gene expression microarray (GSE51546) was down-loaded from the Gene Expression Omnibus. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S08908508183017
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http://dx.doi.org/10.1016/j.mcp.2018.11.003DOI Listing
February 2019
18 Reads

Rapid and sensitive detection of Amphidinium carterae by loop-mediated isothermal amplification combined with a chromatographic lateral-flow dipstick.

Mol Cell Probes 2019 02 9;43:72-79. Epub 2018 Nov 9.

School of Marine Science and Technology, Harbin Institute of Technology (Weihai), Weihai, 264209, PR China.

Frequent outbreaks of toxic algal blooms devastate marine ecosystems, marine fisheries, and public health. Monitoring toxic algae is crucial to reduce losses caused by imminent algal blooms. However, traditional detection techniques relying on morphological examination are tedious and time-consuming. Read More

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http://dx.doi.org/10.1016/j.mcp.2018.11.001DOI Listing
February 2019
2 Reads

Establishment of rapid detection method and surveillance of budgerigar fledgling disease virus using a TaqMan Real-Time PCR.

Mol Cell Probes 2019 02 8;43:80-83. Epub 2018 Nov 8.

Shanghai Key Laboratory of Veterinary Biotechnology, Key Laboratory of Urban Agriculture (South), Ministry of Agriculture, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, 200240, China. Electronic address:

Budgerigar fledgling disease virus (BFDV) infection causes sudden death, abdominal distention, and feather abnormality in psittacine birds. In this study, we developed a TaqMan Real-time PCR assay to detect BFDV by targeting a conserved region in VP1 gene. The detection limit of the assay was 30 DNA gene copies, 1000 times more sensitive than conventional PCR. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S08908508183019
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http://dx.doi.org/10.1016/j.mcp.2018.11.002DOI Listing
February 2019
12 Reads

Reverse transcription recombinase polymerase amplification with lateral flow dipsticks for detection of influenza A virus and subtyping of H1 and H3.

Mol Cell Probes 2018 12 27;42:25-31. Epub 2018 Oct 27.

Ningbo Health BioMed Co., Ltd, Ning Bo, China.

Three reverse transcription recombinase polymerase amplification assays with lateral flow dipsticks (RT-RPA-LFD) were developed for identification of the matrix and hemagglutinin (HA) genes to detect influenza A virus and distinguish subtypes H1 and H3. Assessment of the assays' specificity showed that there was no cross-reactivity with other targets. Their limits of detection were 123. Read More

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http://dx.doi.org/10.1016/j.mcp.2018.10.004DOI Listing
December 2018
4 Reads

Development of double labeling in situ hybridization using RNA probes for genome detection of nervous necrosis virus (NNV).

Mol Cell Probes 2018 12 19;42:18-24. Epub 2018 Oct 19.

Department of Aqualife Medicine, Chonnam National University, Yeosu, Republic of Korea. Electronic address:

In situ hybridization (ISH) of genomic segments using RNA-RNA hybrid for nervous necrosis virus (NNV) detection has not been reported yet. The objective of this study was to develop RNA-ISH using RNA probes for the detection of NNV in infects SSN-1 cells or sevenband grouper Hyporthodus septemfasciatus. Two viral RNA segments viz. Read More

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http://dx.doi.org/10.1016/j.mcp.2018.10.003DOI Listing
December 2018

Bta-miR-130a/b regulates preadipocyte differentiation by targeting PPARG and CYP2U1 in beef cattle.

Mol Cell Probes 2018 12 15;42:10-17. Epub 2018 Oct 15.

College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi, 712100, China; National Beef Cattle Improvement Center, Northwest A&F University, Yangling, Shaanxi, 712100, China. Electronic address:

Deposition of intramuscular fat (IMF) is one of the most important traits for the evaluation of beef carcass quality grade. MicroRNA (miRNA) is an endogenous non-coding RNA that can play a role in the post-transcriptional regulation of mammalian preadipocyte differentiation. Previously, we identified that bta-miR-130a regulates milk fat biosynthesis by targeting PPARG mRNA. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S08908508183021
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http://dx.doi.org/10.1016/j.mcp.2018.10.002DOI Listing
December 2018
3 Reads
1.850 Impact Factor

One-step multiplex real-time RT-PCR for detection and typing of dengue virus.

Mol Cell Probes 2019 02 3;43:86-91. Epub 2018 Oct 3.

Kogenebiotech Co., Ltd, Seoul, Republic of Korea. Electronic address:

Previous studies reported that severity of dengue is associated with multiple factors, including secondary infection, age, viral load and infecting serotype and genotype. In addition, other studies have reported that a dengue virus-2 (DENV-2) infection is associated with a prognosis of more severe clinical manifestations than DENV-1 and DENV-4 infections. For these reasons, the ability to identify the DENV serotypes is critical for optimal patient diagnosis and epidemiological studies. Read More

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http://dx.doi.org/10.1016/j.mcp.2018.10.001DOI Listing
February 2019
23 Reads

In silico investigation of the prion protein glycosylation profiles in relation to scrapie disease resistance in domestic sheep (Ovis aries).

Mol Cell Probes 2018 12 24;42:1-9. Epub 2018 Sep 24.

Ege University, Faculty of Science, Department of Biology, Molecular Biology Section, Izmir, Turkey. Electronic address:

The prion protein is a membrane-bound glycoprotein which consists mainly α-helix structure. In contrast, the infectious prion protein shows the beta-sheet structure. The prion-associated diseases are all lethal neurodegenerative abnormalities, called transmissible spongiform encephalopathies. Read More

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http://dx.doi.org/10.1016/j.mcp.2018.09.004DOI Listing
December 2018

Gold nanoparticle biosensors, a novel application in gene transformation and expression.

Mol Cell Probes 2018 10 7;41:1-7. Epub 2018 Jul 7.

Department of Biology, Faculty of Sciences, University of Zabol, Zabol, Iran.

The conventional techniques of PCR, Southern blot, northern blot, in situ hybridization, and RNase protection assay have long been used to investigate transformation and expression of genes, but most of them are time-consuming and have relatively low sensitivity. In recent years, applying biosensors for molecular identification of biomolecules has been expanding significantly. Hence in this study, Zabol melon was used as a model plant to introduce new DNA and RNA-based biosensors for confirming gene transformation and expression. Read More

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http://dx.doi.org/10.1016/j.mcp.2018.07.002DOI Listing
October 2018

Construction and rescue of Muscovy duck-origin goose parvovirus from an infectious clone containing an E-box deletion within the left terminal region.

Mol Cell Probes 2018 12 18;42:32-35. Epub 2018 Sep 18.

Institute of Animal Husbandry and Veterinary Medicine, Fujian Academy of Agricultural Sciences, Fuzhou, 350013, China; Fujian Animal Diseases Control Technology Development Center, Fuzhou, 350013, China.

To obtain a deletion mutant of Muscovy duck-origin goose parvovirus (MDGPV) and to analyze its biological characteristics, the pMDGPVPT plasmid, which contains a full-length DNA infectious clone of the MDGPV PT strain, was used in this study as the template. The E-box at nt 315 of the left inverted terminal repeat sequence (L-ITR) was deleted by overlap extension PCR to obtain the infectious recombinant plasmid p-PTΔE. The p-PTΔE plasmid was transfected into 9-day-old non-immune Muscovy duck embryos via the yolk sac and the rescued deletion mutant virus r-PTΔE was generated. Read More

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http://dx.doi.org/10.1016/j.mcp.2018.09.003DOI Listing
December 2018
1 Read

Immunopotentiating effect of Inonotus obliquus fermentation products administered at vaccination in chickens.

Mol Cell Probes 2018 10 15;41:43-51. Epub 2018 Sep 15.

Shandong Key Laboratory of Disease Control and Breeding, Institute of Animal Science and Veterinary Medicine, Shandong Academy of Agricultural Science, No.8, Sangyuan Road, Jinan, Shandong, China. Electronic address:

Vaccination is an important approach for the control of avian viral diseases. The aim of this study was to evaluate the immune-potentiating effect of oral administration of Inonotus obliquus fermentation products (IOFP) at vaccination in chickens. In total, 120 one-day-old specific-pathogen-free chickens were randomly assigned to six groups: groups 1 to 3 were vaccinated with Newcastle disease virus (NDV) LaSota live vaccine via intranasal and eye-dropped route at seven days of age, and boosted two weeks later. Read More

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http://dx.doi.org/10.1016/j.mcp.2018.09.002DOI Listing
October 2018
8 Reads

Expression profile of the CSF3 and LPO genes in milk from buffalo (Bubalus bubalis) with and without mastitis.

Mol Cell Probes 2018 10 12;41:39-42. Epub 2018 Sep 12.

Department of Animal Science, Faculty of Agrarian and Veterinary Sciences, Sao Paulo State University, Via de Acesso Prof. Paulo Donato Castellane s/n, 14884-900, Jaboticabal, SP, Brazil. Electronic address:

This study compared the expression profile of the candidate genes, CSF3 and LPO, by investigating the immune response mechanisms involved in the phenotype of resistance and susceptibility to mastitis of healthy and infected buffaloes. The Granulocyte Colony Stimulating Factor 3 (CSF3) and Lactoperoxidase (LPO) genes expression profiles were determined in 24 milk samples from buffaloes with (N = 12) and without (N = 12) mastitis, using the quantitative real-time PCR (qRT-PCR) technique. CSF3 and LPO expressions were 5. Read More

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http://dx.doi.org/10.1016/j.mcp.2018.09.001DOI Listing
October 2018
2 Reads

Development of propidium monoazide-recombinase polymerase amplification (PMA-RPA) assay for rapid detection of Streptococcus pyogenes and Streptococcus agalactiae.

Mol Cell Probes 2018 10 29;41:32-38. Epub 2018 Aug 29.

Shenzhen Academy of Metrology and Quality Inspection/ National Nutrition Food Testing Center (Guangdong), Shenzhen 518000, China. Electronic address:

Streptococcus pyogenes (Group A Streptococcus, GAS) and Streptococcus agalactiae (Group B Streptococcus, GBS) are common pathogens that threaten public health. In this study, a double recombinase polymerase (RPA) amplification assay was developed to rapidly detect these pathogens. Specificity tests revealed that the GAS and GBS strains were positive for speB and SIP genes, respectively. Read More

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http://dx.doi.org/10.1016/j.mcp.2018.08.007DOI Listing
October 2018
13 Reads

Development of a reverse transcription recombinase polymerase amplification assay for rapid detection of Theiler's murine encephalomyelitis virus.

Mol Cell Probes 2018 10 26;41:27-31. Epub 2018 Aug 26.

Guangdong Key Laboratory of Laboratory Animals, Guangdong Laboratory Animals, Monitoring Institute, Guangzhou, China. Electronic address:

Theiler's murine encephalomyelitis virus (TMEV) is one of the most common viral pathogens that circulate widely in captive mouse colonies. A molecular biology detection method would be a useful tool to use in an integrated program to monitor and prevent TMEV infection and transmission. Thus, a reverse transcription recombinase polymerase amplification (RT-RPA) assay was developed to detect TMEV infection. Read More

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http://dx.doi.org/10.1016/j.mcp.2018.08.006DOI Listing
October 2018
26 Reads

Development and validation of a TTR-specific copy number screening tool, and application to potentially relevant patient cohorts.

Mol Cell Probes 2018 10 21;41:61-63. Epub 2018 Aug 21.

Department of Clinical Chemistry and Laboratory Medicine, Jena University Hospital, Am Klinikum 1, 07747, Jena, Germany. Electronic address:

TTR amyloidosis (ATTR) is a fatal condition caused by extracellular deposits of misfolded transthyretin. Patients often present with cardiac disease, but manifestations may also involve other organs including the peripheral nervous system. ATTR is considered familial when heterozygous mutations in the TTR gene are present (ATTRmutant or ATTRm), or acquired when no TTR aberrations are detected (ATTRwildtype or ATTRwt). Read More

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http://dx.doi.org/10.1016/j.mcp.2018.08.005DOI Listing
October 2018
14 Reads

A lateral flow dipstick combined with reverse transcription recombinase polymerase amplification for rapid and visual detection of the bovine respirovirus 3.

Mol Cell Probes 2018 10 20;41:22-26. Epub 2018 Aug 20.

Key Laboratory of Animal Resistant Biology of Shandong, Ruminant Disease Research Center, College of Life Science, Shandong Normal University, Shandong Province, China. Electronic address:

Bovine respirovirus 3 also known as Bovine parainfluenza virus type 3 (BPIV3) is one of the most important viral respiratory agents of both young and adult cattle. Rapid diagnosis could contribute greatly in containing epidemics and thus avoid economic losses. However, the lack of robust isothermal visual method poses difficulty. Read More

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http://dx.doi.org/10.1016/j.mcp.2018.08.004DOI Listing
October 2018
7 Reads

A real time high-resolution melting PCR assay for detection and differentiation among sheep pox virus, goat pox virus, field and vaccine strains of lumpy skin disease virus.

Mol Cell Probes 2018 10 10;41:57-60. Epub 2018 Aug 10.

Federal Center for Animal Health "FGBI ARRIAH", Vladimir, 600901, Russia. Electronic address:

In this paper, we report on the development of a real time high-resolution melting (HRM) PCR assay for detection and differentiation among sheep pox virus (SPPV), goat pox virus (GTPV), field isolates and vaccine strains of lumpy skin disease virus (LSDV) based on high-resolution melting curve analysis of their target PCR amplicons. A 111 bp region of LSDV010 ORF, which harbors unique genetic differences for each of these viral species, was selected as the PCR target in this study. During the validation of this assay using DNA from clinical isolates originated from naturally infected animals from the different geographic locations and reference strains, the obtained PCR amplicons demonstrated that the melting temperature picks were specific for each tested viral species, i. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S08908508183014
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http://dx.doi.org/10.1016/j.mcp.2018.08.003DOI Listing
October 2018
23 Reads

Validation of high-resolution melting analysis as a diagnostic tool for endothelin receptor B mutation in American Paint horses and allele frequency estimation.

Mol Cell Probes 2018 10 8;41:52-56. Epub 2018 Aug 8.

Department of Veterinary Clinical Science, School of Veterinary Medicine and Animal Science, UNESP - Univ Estadual Paulista, Botucatu, SP, Brazil. Electronic address:

Overo lethal white foal syndrome (OLWFS) is a genetic disorder caused by a dinucleotide mutation in the endothelin receptor type B (EDNRB) gene leading to the death of affected foals shortly after birth. The use of rapid and reliable genetic testing is imperative for the early diagnosis of the mutation avoiding, therefore, either additional suffering or the production of affected animals. In the present study, we developed and validated a high-resolution melting (HRM) genotyping assay to detect the OLWFS causative mutation, and we also determined the frequency of heterozygotes among American Paint horses in Brazil. Read More

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http://dx.doi.org/10.1016/j.mcp.2018.08.002DOI Listing
October 2018
1 Read