22,518 results match your criteria Molecular and Cellular Biology[Journal]


Constitutive p38 activation caused inactivation of CRE transcription is mediated by hyper-phosphorylation dependent CRTC2 nucleocytoplasmic transport.

Mol Cell Biol 2019 Feb 19. Epub 2019 Feb 19.

State Key Laboratory of Cellular Stress Biology, Innovation Center for Cell Signaling Network, School of Life Sciences, Xiamen University, Xiamen, Fujian 361105, China

The p38 signal transduction pathway can be activated transiently or constitutively depending on the contexts in which the activation occurs. However, the biological consequence of constitutive activation of p38 is largely unknown. After screening 300 transcriptional co-factors, we identified CRTC2 as a downstream substrate of constitutively activated p38. Read More

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http://dx.doi.org/10.1128/MCB.00554-18DOI Listing
February 2019

Cell cycle progression regulates biogenesis and cellular localization of lipid droplets.

Mol Cell Biol 2019 Feb 19. Epub 2019 Feb 19.

Program of Immunology and Tumor Biology, Brazilian National Cancer Institute (INCA), Rio de Janeiro, RJ, Brazil.

Intracellular lipid accumulation has been associated with poor prognosis in cancer. We have previously reported the involvement of lipid droplets in cell proliferation in colon cancer cells, suggesting a role for these organelles in cancer development. Here, we evaluate the role of lipid droplets in cell cycle regulation and cellular transformation. Read More

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http://dx.doi.org/10.1128/MCB.00374-18DOI Listing
February 2019

The Molecular Chaperone HSP70 Controls Liver Cancer Initiation and Progression by Regulating Adaptive DNA-Damage and MAPK/ERK Signaling Pathways.

Mol Cell Biol 2019 Feb 11. Epub 2019 Feb 11.

Molecular Chaperone Biology, Medical College of Georgia, Georgia Cancer Center, Department of Medicine, and Department of Radiology and Imaging, Augusta University, Augusta, GA 30912

Delineating the mechanisms that drive hepatic injury and hepatocellular carcinoma (HCC) progression is critical for development of novel treatments for recurrent and advanced HCC, but also diagnostic and preventive strategies. Heat shock protein 70 (HSP70) acts in concert with several co-chaperones and nucleotide exchange factors and plays an essential role in protein quality control that increases survival by protecting cells against environmental stressors. Specifically, HSP70-mediated response has been implicated in the pathogenesis of cancer, but the specific mechanisms by which HSP70 may support malignant cell transformation remains to be fully elucidated. Read More

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http://dx.doi.org/10.1128/MCB.00391-18DOI Listing
February 2019

Distinct functions of the cap-binding complex in stimulation of nuclear mRNA export.

Mol Cell Biol 2019 Feb 11. Epub 2019 Feb 11.

Department of Biochemistry and Molecular Biology, Southern Illinois University School of Medicine, Carbondale, IL-62901, USA

Cap-binding complex (CBC) associates co-transcriptionally with the cap-structure at the 5'-end of nascent mRNA to protect it from degradation. Here, we show that CBC promotes the targeting of mRNA export adaptor, Yra1 (that forms transcription-export or TREX complex with THO and Sub2), to the active genes to enhance mRNA export in Likewise, recruitment of Npl3 (a heteronuclear ribonuclear protein involved in mRNA export via formation of export-competent ribonuclear protein complex or RNP) to the active genes is facilitated by CBC. Thus, CBC enhances targeting of the export factors to promote mRNA export. Read More

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http://dx.doi.org/10.1128/MCB.00540-18DOI Listing
February 2019

Transfer RNA Genes Affect Chromosome Structure and Function via Local Effects.

Mol Cell Biol 2019 Feb 4. Epub 2019 Feb 4.

Department of MCD Biology, 1156 High Street, University of California, Santa Cruz, CA 95064 USA

The genome is packaged and organized in an ordered, non-random manner and specific chromatin segments contact nuclear substructures to mediate this organization. Transfer RNA genes (tDNAs) are binding sites for transcription factors and architectural proteins and are thought to play an important role in the organization of the genome. In this study, we investigate the role of tDNAs in genomic organization and chromosome function by editing a chromosome so that it lacks any tDNAs. Read More

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http://dx.doi.org/10.1128/MCB.00432-18DOI Listing
February 2019

Zfp423 Regulates Skeletal Muscle Regeneration and Proliferation.

Mol Cell Biol 2019 Jan 28. Epub 2019 Jan 28.

Department of Medicine, Harvard Medical School, Boston, Massachusetts, USA

Satellite cells (SC) are skeletal muscle stem cells that proliferate in response to injury and provide myogenic precursors for growth and repair. Zfp423 is a transcriptional cofactor expressed in multiple immature cell populations, such as neuronal precursors, mesenchymal stem cells and preadipocytes, where it regulates lineage allocation, proliferation and differentiation. Here, we show that Zfp423 regulates myogenic progression during muscle regeneration. Read More

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http://dx.doi.org/10.1128/MCB.00447-18DOI Listing
January 2019
2 Reads

Reactive Oxygen Species Signaling Promotes HIF1α Stabilization in Sonic Hedgehog-Driven Cerebellar Progenitor Cell Proliferation.

Mol Cell Biol 2019 Jan 28. Epub 2019 Jan 28.

Department of Pediatric Oncology, Emory University School of Medicine, Atlanta, Georgia, USA

Cerebellar development is a highly regulated process involving numerous factors acting with high specificity, both temporally and by location. Part of this process involves extensive proliferation of cerebellar granule neuron precursors (CGNPs) induced by Sonic Hedgehog (SHH) signaling, but downstream effectors of mitogenic signaling are still being elucidated. Using primary CGNP cultures, a well-established model for SHH-driven proliferation, we show that SHH-treated CGNPs feature high levels of Hypoxia-Inducible Factor-1-Alpha (HIF1α), which is known to promote glycolysis, stemness, and angiogenesis. Read More

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http://dx.doi.org/10.1128/MCB.00268-18DOI Listing
January 2019
1 Read

E3 ubiquitin ligases RNF20 and RNF40 are required for DSB repair: evidence for monoubiquitination of histone H2B lysine 120 as a novel axis of DSB signaling and repair.

Mol Cell Biol 2019 Jan 28. Epub 2019 Jan 28.

Department of Immunology, University of Toronto, Toronto, Ontario, Canada

Histone post-translational modifications play fundamental roles in the regulation of double-stranded DNA break (DSB) repair. RNF20/RNF40-mediated monoubiquitination of histone H2B on lysine 120 (H2Bub) has been suggested as a potential mediator of DSB repair, although the nature and function of this post-translational modification remain enigmatic. In this report, we demonstrate that RNF20 and RNF40 are required for DSB repair leading to homologous recombination (HR) and class switch recombination, a process driven by non-homologous end-joining (NHEJ), in mouse B cells. Read More

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http://dx.doi.org/10.1128/MCB.00488-18DOI Listing
January 2019

Dynamic methylation of an L1 transduction family during reprogramming and neurodifferentiation.

Mol Cell Biol 2019 Jan 28. Epub 2019 Jan 28.

Mater Research Institute - University of Queensland, TRI Building, Woolloongabba QLD 4102, Australia.

The retrotransposon LINE-1 (L1) is a significant source of endogenous mutagenesis in humans. In each individual genome, a handful of retrotransposition-competent L1s (RC-L1s) can generate new heritable L1 insertions in the early embryo, primordial germline, and in germ cells. L1 retrotransposition can also occur in the neuronal lineage and cause somatic mosaicism. Read More

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http://mcb.asm.org/lookup/doi/10.1128/MCB.00499-18
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http://dx.doi.org/10.1128/MCB.00499-18DOI Listing
January 2019
3 Reads

IMP2 increases mouse skeletal muscle mass and voluntary activity by enhancing autocrine IGF2 production and optimizing muscle metabolism.

Mol Cell Biol 2019 Jan 28. Epub 2019 Jan 28.

Department of Molecular Biology and the Diabetes and Cardiac units of the Medical Services, Massachusetts General Hospital, Boston, MA 02114 USA

The IGF2 mRNA binding protein2/IMP2 was selectively deleted from adult mouse muscle; two phenotypes were observed: decreased accrual of skeletal muscle mass after weaning and reduced wheel running activity but normal forced treadmill performance. Reduced wheel running occurs when fed a high fat diet but is normalized consuming standard chow. The two phenotypes are due to altered abundance of different IMP2 client mRNAs. Read More

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http://dx.doi.org/10.1128/MCB.00528-18DOI Listing
January 2019
1 Read

Generation and Molecular Characterization of Human Ring Sideroblasts: A Key Role of Ferrous Iron in Terminal Erythroid Differentiation and Ring Sideroblast Formation.

Mol Cell Biol 2019 Jan 22. Epub 2019 Jan 22.

Department of Hematology and Rheumatology, Tohoku University Graduate School of Medicine, Sendai, Japan

Ring sideroblasts are a hallmark of sideroblastic anemia, though little is known about their characteristics. Here, we first generated mutant mice by disrupting the GATA-1 binding motif at the intron 1 enhancer of the gene, a gene responsible for X-linked sideroblastic anemia (XLSA). Although heterozygous female mice showed an anemic phenotype, ring sideroblasts were not observed in their bone marrow. Read More

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http://mcb.asm.org/lookup/doi/10.1128/MCB.00387-18
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http://dx.doi.org/10.1128/MCB.00387-18DOI Listing
January 2019
9 Reads

Increased expression of miR-551a by c-Fos reduces Focal Adhesion Kinase (FAK) levels and blocks tumorigenesis.

Mol Cell Biol 2019 Jan 22. Epub 2019 Jan 22.

Department of Biotechnology, Indian Institute of Technology Madras (IITM), Chennai 600 036, India.

Breast cancer is the recurrent type of cancer amidst women worldwide. Despite a remarkable progress in the prevention, detection and treatment of breast cancer, it still remains as a major chronic problem worldwide and poses significant challenges like metastasis to distant organs - demanding the need for novel biomarkers and therapeutic targets. Focal adhesion kinase (FAK) - a member of the protein tyrosine kinase, has been shown to be expressed in high levels in breast tumors. Read More

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http://mcb.asm.org/lookup/doi/10.1128/MCB.00577-18
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http://dx.doi.org/10.1128/MCB.00577-18DOI Listing
January 2019
6 Reads

Mediator subunit Med1 is required for GATA-dependent developmental processes: divergent binding interfaces for conserved coactivator functions.

Mol Cell Biol 2019 Jan 22. Epub 2019 Jan 22.

Centre de Biologie Intégrative (CBI)-CBD, UMR5547 CNRS/Université Toulouse III, 118 Route de Narbonne, 31062 Toulouse, France.

DNA-bound transcription factors (TFs) governing developmental gene regulation have been proposed to recruit Polymerase II machinery at gene promoters through specific interactions with dedicated subunits of the evolutionarily-conserved Mediator complex (MED). However, whether such MED subunit specific functions and partnerships have been conserved during evolution has been poorly investigated. To address this issue, we generated the first loss-of-function mutants for Med1, known as a specific cofactor for GATA TFs and hormone nuclear receptors in mammals. Read More

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http://dx.doi.org/10.1128/MCB.00477-18DOI Listing
January 2019

Reevaluation of the Role of ERK3 in Perinatal Survival and Post-Natal Growth Using New Genetically-Engineered Mouse Models.

Mol Cell Biol 2019 Jan 14. Epub 2019 Jan 14.

Institute for Research in Immunology and Cancer, Université de Montréal, Montreal, Quebec, Canada

The physiological functions of the atypical MAP kinase ERK3 remain poorly characterized. Previous analysis of mice with a targeted insertion of the reporter in the locus ( ) showed that inactivation of ERK3 in mice leads to perinatal lethality associated with intrauterine growth restriction, defective lung maturation, and neuromuscular anomalies. To further explore the role of ERK3 in physiology and disease, we generated novel mouse models expressing a catalytically-inactive ( ) or conditional ( ) allele of ERK3. Read More

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http://dx.doi.org/10.1128/MCB.00527-18DOI Listing
January 2019

Germline deletion reveals a non-essential role of the atypical MAPK6/ERK3.

Mol Cell Biol 2019 Jan 14. Epub 2019 Jan 14.

Institute of Cell Biochemistry, Hannover Medical School, Hannover, Germany

MAPK6/ERK3 is an atypical member of the MAPKs. An essential role has been suggested by the perinatal lethal phenotype of ERK3 knockout mice carrying a lacZ insertion in exon 2 due to pulmonary disfunction and by defects in function, activation and positive selection of T cells. To study the role of ERK3 , we generated mice carrying a conditional allele with exon3 flanked by LoxP sites. Read More

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http://dx.doi.org/10.1128/MCB.00516-18DOI Listing
January 2019
1 Read

A feedback loop between miRNA-155, Programmed cell death 4 and Activation Protein-1 modulates the expression of miR-155 and tumorigenesis in tongue cancer.

Mol Cell Biol 2019 Jan 7. Epub 2019 Jan 7.

Department of Biotechnology, Bhupat and Jyoti Mehta School of Biosciences, Indian Institute of Technology Madras, Chennai, India

miR-155 is an oncomir, generated as a non-coding RNA from gene whose promoter activity is mainly controlled via AP-1 and NF-κB transcription factors. We found that the expression levels of miR-155 and Pdcd4 exhibit inverse relationship in tongue cancer cells (SAS and AWL) and tumor tissues compared to normal FBM cells and normal tongue tissues, respectively. Insilco and In-vitro studies with 3'UTR of Pdcd4 via luciferase reporter assays, qPCR and western blots show that miR-155 directly targets Pdcd4 mRNA and blocks its expression. Read More

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http://mcb.asm.org/lookup/doi/10.1128/MCB.00410-18
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http://dx.doi.org/10.1128/MCB.00410-18DOI Listing
January 2019
9 Reads

Loss of estrogen-related receptor alpha facilitates angiogenesis in endothelial cells.

Mol Cell Biol 2019 Jan 2. Epub 2019 Jan 2.

Metabolic and Degenerative Diseases, Institute of Molecular Medicine, The University of Texas McGovern Medical School, Houston, TX, USA.

Estrogen-related receptors (ERRs) have emerged as major metabolic regulators in various tissues. However, their expression and function in the vasculature remains unknown. Here we report the transcriptional program and cellular function of ERRα in endothelial cells (ECs), a cell type with a multi-faceted role in vasculature. Read More

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http://dx.doi.org/10.1128/MCB.00411-18DOI Listing
January 2019
2 Reads

CSB-dependent CDK9 degradation and RNA Polymerase II phosphorylation during Transcription Coupled Repair.

Mol Cell Biol 2019 Jan 2. Epub 2019 Jan 2.

Institut NeuroMyoGène (INMG), CNRS UMR 5310, INSERM U1217, Université de Lyon, Université Claude Bernard Lyon1, 16 rue Dubois, 69622 Villeurbanne CEDEX FRANCE

DNA lesions block cellular processes such as transcription, inducing apoptosis, tissue failures and premature ageing. To counteract the deleterious effects of DNA damage, cells are equipped with various DNA repair pathways. Transcription Coupled Repair specifically removes helix-distorting DNA adducts in a coordinated multi-step process. Read More

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http://dx.doi.org/10.1128/MCB.00225-18DOI Listing
January 2019

Common and Differential Transcriptional Actions of Nuclear Receptors Liver X Receptors α and β in Macrophages.

Mol Cell Biol 2019 Mar 15;39(5). Epub 2019 Feb 15.

Instituto de Investigaciones Biomédicas Alberto Sols, Consejo Superior de Investigaciones Científicas (CSIC)-Universidad Autónoma de Madrid, Madrid, Spain

The liver X receptors α and β (LXRα and LXRβ) are oxysterol-activated transcription factors that coordinately regulate gene expression that is important for cholesterol and fatty acid metabolism. In addition to their roles in lipid metabolism, LXRs participate in the transcriptional regulation of macrophage activation and are considered potent regulators of inflammation. LXRs are highly similar, and despite notable exceptions, most of their reported functions are substantially overlapping. Read More

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http://dx.doi.org/10.1128/MCB.00376-18DOI Listing
March 2019
1 Read

RNA binding protein HuR promotes autophagosome formation by regulating expressions of autophagy-related protein 5, 12, and 16 in human hepatocellular carcinoma cells.

Mol Cell Biol 2019 Jan 2. Epub 2019 Jan 2.

Department of Biochemistry, The Catholic University of Korea College of Medicine, Seoul, South Korea

Autophagy is a lysosomal self-degradation process of cellular components by forming autophagosomes. Autophagosome formation is an essential process in autophagy, and is fine-tuned by various autophagy-related gene (ATG) molecules including ATG5, ATG12, and ATG16. Although several reports have shown that numerous factors affect multiple levels of gene regulation to orchestrate cellular autophagy, the detailed mechanism of autophagosome formation still needs further investigation. Read More

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http://dx.doi.org/10.1128/MCB.00508-18DOI Listing
January 2019
1 Read

Rapid recapitulation of non-alcoholic steatohepatitis upon loss of HCF-1 function in mice.

Mol Cell Biol 2018 Dec 17. Epub 2018 Dec 17.

Center for Integrative Genomics, Génopode, University of Lausanne, CH-1015 Lausanne, Switzerland

Host-cell factor 1 (HCF-1), encoded by the ubiquitously expressed X-linked gene , is an epigenetic co-regulator important for mouse development and cell proliferation, including during liver regeneration. We used a hepatocyte-specific inducible knock-out allele (called ), to induce HCF-1 loss in hepatocytes of hemizygous males by four days. In heterozygous females, owing to random X-chromosome inactivation, upon allele induction, a 50/50 mix of HCF-1 positive and negative hepatocyte clusters is engineered. Read More

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http://mcb.asm.org/lookup/doi/10.1128/MCB.00405-18
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http://dx.doi.org/10.1128/MCB.00405-18DOI Listing
December 2018
9 Reads

G-Alpha Subunit Abundance and Activity Differentially Regulate β-Catenin Signaling.

Mol Cell Biol 2019 Mar 15;39(5). Epub 2019 Feb 15.

Centre for Craniofacial and Regenerative Biology, King's College London, Guy's Hospital, London, United Kingdom

Heterotrimeric G proteins are signal transduction proteins involved in regulating numerous signaling events. In particular, previous studies have demonstrated a role for G-proteins in regulating β-catenin signaling. However, the link between G-proteins and β-catenin signaling is controversial and appears to depend on G-protein specificity. Read More

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http://dx.doi.org/10.1128/MCB.00422-18DOI Listing

Role of SMPD3 during Bone Fracture Healing and Regulation of Its Expression.

Mol Cell Biol 2019 Feb 4;39(4). Epub 2019 Feb 4.

Faculty of Dentistry, McGill University, Montreal, Quebec, Canada

Sphingomyelin phosphodiesterase 3 (SMPD3), a lipid-metabolizing enzyme present in bone and cartilage, has important roles in the developing skeleton. We previously showed that SMPD3 deficiency results in delayed extracellular matrix (ECM) mineralization and severe skeletal deformities in an inducible knockout mouse model, ; mice, in which was ablated in -expressing chondrocytes and osteoblasts during early skeletogenesis. However, as shown in the current study, ablation of postnatally in 3-month-old ; mice resulted in only a mild bone mineralization defect. Read More

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http://dx.doi.org/10.1128/MCB.00370-18DOI Listing
February 2019

Yeast Mpo1 Is a Novel Dioxygenase That Catalyzes the α-Oxidation of a 2-Hydroxy Fatty Acid in an Fe-Dependent Manner.

Mol Cell Biol 2019 Mar 15;39(5). Epub 2019 Feb 15.

Laboratory of Biochemistry, Faculty of Pharmaceutical Sciences, Hokkaido University, Sapporo, Japan

Phytosphingosine (PHS) is the major long-chain base component of sphingolipids in The PHS metabolic pathway includes a fatty acid (FA) α-oxidation reaction. Recently, we identified the novel protein Mpo1, which is involved in PHS metabolism. However, the details of the FA α-oxidation reaction and the role of Mpo1 in PHS metabolism remained unclear. Read More

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http://mcb.asm.org/lookup/doi/10.1128/MCB.00428-18
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http://dx.doi.org/10.1128/MCB.00428-18DOI Listing
March 2019
4 Reads

Both a Unique Motif at the C Terminus and an N-Terminal HEAT Repeat Contribute to G-Quadruplex Binding and Origin Regulation by the Rif1 Protein.

Mol Cell Biol 2019 Feb 4;39(4). Epub 2019 Feb 4.

Department of Genome Medicine, Tokyo Metropolitan Institute of Medical Science, Kamikitazawa, Setagaya-ku, Tokyo, Japan

Rif1 is a key factor for spatiotemporal regulation of DNA replication. Rif1 suppresses origin firing in the mid-late replication domains by generating replication-suppressive chromatin architecture and by recruiting a protein phosphatase. In fission yeast, the function of Hsk1, a kinase important for origin firing, can be bypassed by Δ due to the loss of origin suppression. Read More

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http://mcb.asm.org/lookup/doi/10.1128/MCB.00364-18
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http://dx.doi.org/10.1128/MCB.00364-18DOI Listing
February 2019
28 Reads

hnRNP Q regulates IRES-mediated translation in neurons.

Mol Cell Biol 2018 Nov 26. Epub 2018 Nov 26.

Department of Life Sciences, Pohang University of Science and Technology, Pohang 37673, Korea.

Fragile X syndrome (FXS) caused by loss of fragile X mental retardation protein (FMRP) is the most common cause of inherited intellectual disability. Numerous studies show that FMRP is a RNA binding protein that regulates translation of its binding targets and plays key roles in neuronal functions. However, the regulatory mechanism for FMRP expression is incompletely understood. Read More

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http://dx.doi.org/10.1128/MCB.00371-18DOI Listing
November 2018
1 Read

Mutant p53 sequestration of MDM2 acidic domain inhibits E3 ligase activity.

Mol Cell Biol 2018 Nov 19. Epub 2018 Nov 19.

Molecular Oncology Department, Moffitt Cancer Center, 12902 Magnolia Drive, Tampa, FL 33612, USA

Missense p53 mutants often accumulate in tumors and drive progression through gain-of-function. MDM2 efficiently degrades wild type p53, but fails to degrade mutant p53 in tumor cells. Previous studies revealed that mutant p53 inhibits MDM2 auto-ubiquitination, suggesting that the interaction inhibits MDM2 E3 activity. Read More

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http://dx.doi.org/10.1128/MCB.00375-18DOI Listing
November 2018

A Novel Regulatory Axis, CHD1L-MicroRNA 486-Matrix Metalloproteinase 2, Controls Spermatogonial Stem Cell Properties.

Mol Cell Biol 2019 Feb 4;39(4). Epub 2019 Feb 4.

Affiliated Cancer Hospital & Institute of Guangzhou Medical University, Xinzao Town, Panyu District, Guangzhou, Guangdong, China

Spermatogonial stem cells (SSCs) are unipotent germ cells that are at the foundation of spermatogenesis and male fertility. However, the underlying molecular mechanisms governing SSC stemness and growth properties remain elusive. We have recently identified chromodomain helicase/ATPase DNA binding protein 1-like (Chd1l) as a novel regulator for SSC survival and self-renewal, but how these functions are controlled by Chd1l remains to be resolved. Read More

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http://mcb.asm.org/lookup/doi/10.1128/MCB.00357-18
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http://dx.doi.org/10.1128/MCB.00357-18DOI Listing
February 2019
20 Reads

The Phosphorylated Estrogen Receptor Cistrome Identifies a Subset of Active Enhancers Enriched for Direct ER-DNA Binding and the Transcription Factor GRHL2.

Mol Cell Biol 2018 Nov 19. Epub 2018 Nov 19.

McArdle Laboratory for Cancer Research, Department of Oncology and Carbone Comprehensive Cancer Center, University of Wisconsin - Madison, Madison, WI

Post-translational modifications are key regulators of protein function, providing cues that can alter protein interactions and cellular location. Phosphorylation of the estrogen receptor (ER) at serine 118 (pS118-ER) occurs in response to multiple stimuli and is involved in modulating ER-dependent gene transcription. While the cistrome of ER is well established, surprisingly little is understood about how phosphorylation impacts ER-DNA binding activity. Read More

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http://dx.doi.org/10.1128/MCB.00417-18DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6336141PMC
November 2018
9 Reads

Cancer-Associated Eukaryotic Translation Initiation Factor 1A Mutants Impair Rps3 and Rps10 Binding and Enhance Scanning of Cell Cycle Genes.

Mol Cell Biol 2019 Feb 16;39(3). Epub 2019 Jan 16.

Department of Biomolecular Sciences, The Weizmann Institute of Science, Rehovot, Israel

Protein synthesis is linked to cell proliferation, and its deregulation contributes to cancer. Eukaryotic translation initiation factor 1A (eIF1A) plays a key role in scanning and AUG selection and differentially affects the translation of distinct mRNAs. Its unstructured N-terminal tail (NTT) is frequently mutated in several malignancies. Read More

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http://mcb.asm.org/lookup/doi/10.1128/MCB.00441-18
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http://dx.doi.org/10.1128/MCB.00441-18DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6336143PMC
February 2019
4 Reads

Nuclear Phosphatidylinositol 5-Phosphatase Is Essential for Allelic Exclusion of Variant Surface Glycoprotein Genes in Trypanosomes.

Mol Cell Biol 2019 Feb 16;39(3). Epub 2019 Jan 16.

Center for Infectious Disease Research, Seattle, Washington, USA

Allelic exclusion of variant surface glycoprotein (VSG) genes is essential for African trypanosomes to evade the host antibody response by antigenic variation. The mechanisms by which this parasite expresses only one of its ∼2,000 VSG genes at a time are unknown. We show that nuclear phosphatidylinositol 5-phosphatase (PIP5Pase) interacts with repressor activator protein 1 (RAP1) in a multiprotein complex and functions in the control of VSG allelic exclusion. Read More

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http://dx.doi.org/10.1128/MCB.00395-18DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6336139PMC
February 2019
1 Read

Senescent Breast Luminal Cells Promote Carcinogenesis through Interleukin-8-Dependent Activation of Stromal Fibroblasts.

Mol Cell Biol 2019 Jan 3;39(2). Epub 2019 Jan 3.

Department of Molecular Oncology, King Faisal Specialist Hospital and Research Centre, Riyadh, Kingdom of Saudi Arabia

Aging and stress promote senescence, which has intrinsic tumor suppressor functions and extrinsic tumor promoting properties. Therefore, it is of utmost importance to delineate the effects of senescence inducers on the various types of cells that compose the different organs. We show here that primary normal breast luminal (NBL) cells are more sensitive than their corresponding stromal fibroblasts to proliferative as well as oxidative damage-induced senescence. Read More

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http://mcb.asm.org/lookup/doi/10.1128/MCB.00359-18
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http://dx.doi.org/10.1128/MCB.00359-18DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6321881PMC
January 2019
18 Reads
4.780 Impact Factor

Coordinated Regulation of Intracellular Fascin Distribution Governs Tumor Microvesicle Release and Invasive Cell Capacity.

Mol Cell Biol 2019 Feb 16;39(3). Epub 2019 Jan 16.

Department of Biological Sciences, University of Notre Dame, Notre Dame, Indiana, USA

Tumor cell invasion is one result of the bidirectional interactions occurring between tumor cells and the surrounding milieu. The ability of tumor cells to invade through the extracellular matrix is in part regulated by the formation of a class of protease-loaded extracellular vesicles, called tumor microvesicles (TMVs), which are released directly from the cell surface. Here we show that the actin bundling protein, fascin, redistributes to the cell periphery in a ternary complex with podocalyxin and ezrin, where it promotes TMV release. Read More

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http://mcb.asm.org/lookup/doi/10.1128/MCB.00264-18
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http://dx.doi.org/10.1128/MCB.00264-18DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6336144PMC
February 2019
12 Reads

SNW1, a Novel Transcriptional Regulator of the NF-κB Pathway.

Mol Cell Biol 2019 Feb 16;39(3). Epub 2019 Jan 16.

Salk Institute for Biological Studies, La Jolla, California, USA

The nuclear factor kappa B (NF-κB) family of transcription factors plays a central role in coordinating the expression of genes that control inflammation, immune responses, cell proliferation, and a variety of other biological processes. In an attempt to identify novel regulators of this pathway, we performed whole-genome RNA interference (RNAi) screens in physiologically relevant human macrophages in response to lipopolysaccharide and tumor necrosis factor alpha (TNF-α). The top hit was SNW1, a splicing factor and transcriptional coactivator. Read More

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http://dx.doi.org/10.1128/MCB.00415-18DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6336138PMC
February 2019
1 Read

Novel Lines of Evidence for the Asymmetric Strand Displacement Model of Mitochondrial DNA Replication.

Authors:
Chih-Lin Hsieh

Mol Cell Biol 2019 Jan 3;39(2). Epub 2019 Jan 3.

Norris Comprehensive Cancer Center, University of Southern California, Los Angeles, California, USA

The mitochondrial genome, which consists of 16,569 bp of DNA with a cytosine-rich light (L) strand and a heavy (H) strand, exists as a multicopy closed circular genome within the mitochondrial matrix. The machinery for replication of the mammalian mitochondrial genome is distinct from that for replication of the nuclear genome. Three models have been proposed for mitochondrial DNA (mtDNA) replication, and one of the key differences among them is whether extensive single-stranded regions exist on the H strand. Read More

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http://mcb.asm.org/lookup/doi/10.1128/MCB.00406-18
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http://dx.doi.org/10.1128/MCB.00406-18DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6321883PMC
January 2019
11 Reads

Hypoxia Restrains Lipid Utilization via Protein Kinase A and ATGL Downregulation through Hypoxia Inducible Factor.

Mol Cell Biol 2018 Nov 5. Epub 2018 Nov 5.

National Creative Research Initiatives Center for Adipose Tissue Remodeling, Institute of Molecular Biology and Genetics, Department of Biological Sciences, Seoul National University, Seoul 08826, South Korea

Oxygen is a key molecule for efficient energy production in living organisms. Although aerobic organisms have adaptive processes to survive in low-oxygen environments, it is poorly understood how lipolysis, the first step of energy production from stored lipid metabolites, would be modulated during hypoxia. Here, we demonstrate that fasting-induced lipolysis is downregulated by hypoxia through the hypoxia-inducible factor (HIF) signaling pathway. Read More

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http://mcb.asm.org/lookup/doi/10.1128/MCB.00390-18
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http://dx.doi.org/10.1128/MCB.00390-18DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6321877PMC
November 2018
2 Reads

Interleukin-8 Activates Breast Cancer-Associated Adipocytes and Promotes Their Angiogenesis- and Tumorigenesis-Promoting Effects.

Mol Cell Biol 2019 Jan 3;39(2). Epub 2019 Jan 3.

Department of Molecular Oncology, King Faisal Specialist Hospital and Research Centre, Riyadh, Kingdom of Saudi Arabia

Increasing evidence supports the critical role of active stromal adipocytes in breast cancer development and spread. However, the mediators and the mechanisms of action are still elusive. We show here that cancer-associated adipocytes (CAAs) isolated from 10 invasive breast carcinomas are proinflammatory and exhibit active phenotypes, including higher proliferative, invasive, and migratory capacities compared to their adjacent tumor-counterpart adipocytes (TCAs). Read More

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http://mcb.asm.org/lookup/doi/10.1128/MCB.00332-18
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http://dx.doi.org/10.1128/MCB.00332-18DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6321878PMC
January 2019
19 Reads
4.780 Impact Factor

DDX3 Participates in Translational Control of Inflammation Induced by Infections and Injuries.

Mol Cell Biol 2019 Jan 11;39(1). Epub 2018 Dec 11.

Department of Biomedical Sciences, Chang Gung University, Taoyuan, Taiwan

Recent studies have suggested that DDX3 functions in antiviral innate immunity, but the underlying mechanism remains elusive. We previously identified target mRNAs whose translation is controlled by DDX3. Pathway enrichment analysis of these targets indicated that DDX3 is involved in various infections and inflammation. Read More

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http://aem.asm.org/lookup/doi/10.1128/MCB.00285-18
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http://dx.doi.org/10.1128/MCB.00285-18DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6290373PMC
January 2019
15 Reads

Specific Modification of Aged Proteasomes Revealed by Tag-Exchangeable Knock-In Mice.

Mol Cell Biol 2019 Jan 11;39(1). Epub 2018 Dec 11.

Laboratory of Protein Metabolism, Graduate School of Pharmaceutical Sciences, The University of Tokyo, Tokyo, Japan

The proteasome is the proteolytic machinery at the center of regulated intracellular protein degradation and participates in various cellular processes. Maintaining the quality of the proteasome is therefore important for proper cell function. It is unclear, however, how proteasomes change over time and how aged proteasomes are disposed. Read More

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http://mcb.asm.org/lookup/doi/10.1128/MCB.00426-18
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http://dx.doi.org/10.1128/MCB.00426-18DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6290375PMC
January 2019
7 Reads

Brc1 Promotes the Focal Accumulation and SUMO Ligase Activity of Smc5-Smc6 During Replication Stress.

Mol Cell Biol 2018 Oct 22. Epub 2018 Oct 22.

Department of Molecular Medicine, The Scripps Research Institute, La Jolla, CA 92037, USA.

As genetic instability drives disease or loss of cell fitness, cellular safeguards have evolved to protect the genome, especially during sensitive cell cycle phases such as DNA replication. Fission yeast Brc1 has emerged as a key factor in promoting cell survival when replication forks are stalled or collapsed. Brc1 is a multi-BRCT protein that is structurally related to the budding yeast Rtt107 and human PTIP DNA damage response factors, but functional similarities appear limited. Read More

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http://dx.doi.org/10.1128/MCB.00271-18DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6321882PMC
October 2018
8 Reads

A Heterologous Cell Model for Studying the Role of T-Cell Intracellular Antigen 1 in Welander Distal Myopathy.

Mol Cell Biol 2019 Jan 11;39(1). Epub 2018 Dec 11.

Centro de Biología Molecular Severo Ochoa, Consejo Superior de Investigaciones Científicas, Universidad Autónoma de Madrid, Madrid, Spain

Welander distal myopathy (WDM) is a muscle dystrophy characterized by adult-onset distal muscle weakness, prevalently impacting the distal long extensors of the hands and feet. WDM is an autosomal dominant disorder caused by a missense mutation (c.1362G>A; p. Read More

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http://dx.doi.org/10.1128/MCB.00299-18DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6290374PMC
January 2019
8 Reads

A Tumor-Promoting Phorbol Ester Causes a Large Increase in APOBEC3A Expression and a Moderate Increase in APOBEC3B Expression in a Normal Human Keratinocyte Cell Line without Increasing Genomic Uracils.

Mol Cell Biol 2019 Jan 11;39(1). Epub 2018 Dec 11.

Department of Chemistry, Wayne State University, Detroit, Michigan, USA

Phorbol 12-myristate 13-acetate (PMA) promotes skin cancer in rodents. The mutations found in murine tumors are similar to those found in human skin cancers, and PMA promotes proliferation of human skin cells. PMA treatment of human keratinocytes increases the synthesis of APOBEC3A, an enzyme that converts cytosines in single-stranded DNA to uracil, and mutations in a variety of human cancers are attributed to APOBEC3A or APOBEC3B expression. Read More

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http://mcb.asm.org/lookup/doi/10.1128/MCB.00238-18
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http://dx.doi.org/10.1128/MCB.00238-18DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6290372PMC
January 2019
8 Reads

Inner Mitochondrial Translocase Tim50 Is Central in Adrenal and Testicular Steroid Synthesis.

Mol Cell Biol 2019 Jan 11;39(1). Epub 2018 Dec 11.

Department of Chemistry, University of Alberta, Edmonton, Alberta, Canada.

Adrenal and gonadal mitochondrial metabolic activity requires electrons from cofactors, cholesterol, and a substrate for rapid steroid synthesis, an essential requirement for mammalian survival. Substrate activity depends on its environment, which is regulated by chaperones and mitochondrial translocases. Cytochrome P450 side-chain cleavage enzyme (SCC or CYP11A1) catalyzes cholesterol to pregnenolone conversion, although its mechanism of action is not well understood. Read More

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http://mcb.asm.org/lookup/doi/10.1128/MCB.00484-18
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http://dx.doi.org/10.1128/MCB.00484-18DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6290369PMC
January 2019
9 Reads

Phosphorylation of TIP60 suppresses 53BP1 localization at DNA damage sites.

Mol Cell Biol 2018 Oct 8. Epub 2018 Oct 8.

Department of Cancer Biology, Basser Center for BRCA, Abramson Family Cancer Research Institute, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA 19146

Proper balance between the repair of DNA double-strand breaks (DSBs) by homologous recombination and nonhomologous end-joining is critical for maintaining genome integrity and preventing tumorigenesis. This balance is regulated and fine-tuned by a variety of factors, including cell cycle and the chromatin environment. The histone acetyltransferase TIP60 was previously shown to suppress pathologic end-joining and promote homologous recombination. Read More

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http://mcb.asm.org/lookup/doi/10.1128/MCB.00209-18
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http://dx.doi.org/10.1128/MCB.00209-18DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6290371PMC
October 2018
1 Read
4.777 Impact Factor

Hsp70 Interacts with Mitogen-Activated Protein Kinase (MAPK)-Activated Protein Kinase 2 To Regulate p38MAPK Stability and Myoblast Differentiation during Skeletal Muscle Regeneration.

Mol Cell Biol 2018 Dec 28;38(24). Epub 2018 Nov 28.

Department of Biochemistry and Molecular Biology, Zhejiang University School of Medicine, Hangzhou, China

The regenerative process of injured muscle is dependent on the fusion and differentiation of myoblasts derived from muscle stem cells. Hsp70 is important for maintaining skeletal muscle homeostasis and regeneration, but the precise cellular mechanism remains elusive. In this study, we found that Hsp70 was upregulated during myoblast differentiation. Read More

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http://mcb.asm.org/lookup/doi/10.1128/MCB.00211-18
Publisher Site
http://dx.doi.org/10.1128/MCB.00211-18DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6275188PMC
December 2018
11 Reads

Mediator Is Essential for Small Nuclear and Nucleolar RNA Transcription in Yeast.

Mol Cell Biol 2018 Dec 28;38(24). Epub 2018 Nov 28.

Department of Biology, Indiana University, Bloomington, Indiana, USA

Eukaryotic RNA polymerase II (RNAPII) transcribes mRNA genes and non-protein-coding RNA (ncRNA) genes, including those encoding small nuclear and nucleolar RNAs (sn/snoRNAs). In metazoans, RNAPII transcription of sn/snoRNAs is facilitated by a number of specialized complexes, but no such complexes have been discovered in yeast. It has been proposed that yeast sn/snoRNA and mRNA expression relies on a set of common factors, but the extent to which regulators of mRNA genes function at yeast sn/snoRNA genes is unclear. Read More

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http://mcb.asm.org/lookup/doi/10.1128/MCB.00296-18
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http://dx.doi.org/10.1128/MCB.00296-18DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6275182PMC
December 2018
6 Reads

New Player in Endosomal Trafficking: Differential Roles of Smad Anchor for Receptor Activation (SARA) Protein.

Mol Cell Biol 2018 Dec 28;38(24). Epub 2018 Nov 28.

Universidad Nacional de Córdoba (UNC), Ciudad Universitaria, Córdoba, Argentina

The development and maintenance of multicellular organisms require specialized coordination between external cellular signals and the proteins receiving stimuli and regulating responses. A critical role in the proper functioning of these processes is played by endosomal trafficking, which enables the transport of proteins to targeted sites as well as their return to the plasma membrane through its essential components, the endosomes. During this trafficking, signaling pathways controlling functions related to the endosomal system are activated both directly and indirectly. Read More

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http://mcb.asm.org/lookup/doi/10.1128/MCB.00446-18
Publisher Site
http://dx.doi.org/10.1128/MCB.00446-18DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6275186PMC
December 2018
1 Read

Expression of the Alternative Oxidase Influences Jun N-Terminal Kinase Signaling and Cell Migration.

Mol Cell Biol 2018 Dec 28;38(24). Epub 2018 Nov 28.

Faculty of Medicine and Life Sciences, University of Tampere, Tampere, Finland

Downregulation of Jun N-terminal kinase (JNK) signaling inhibits cell migration in diverse model systems. In pupal development, attenuated JNK signaling in the thoracic dorsal epithelium leads to defective midline closure, resulting in cleft thorax. Here we report that concomitant expression of the alternative oxidase (AOX) was able to compensate for JNK pathway downregulation, substantially correcting the cleft thorax phenotype. Read More

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http://dx.doi.org/10.1128/MCB.00110-18DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6275184PMC
December 2018
2 Reads