22,533 results match your criteria Molecular and Cellular Biology[Journal]


Growth Factor Independence (GFI) 1B-mediated transcriptional repression and lineage allocation require Lysine Specific Demethylase (LSD)1-dependent recruitment of the BHC complex.

Mol Cell Biol 2019 Apr 15. Epub 2019 Apr 15.

Department of Oncological Sciences, University of Utah School of Medicine, Salt Lake City, UT, U.S.A.

Growth Factor Independence (GFI)1B coordinates assembly of transcriptional repressor complexes comprised of co-repressors and histone modifying enzymes to control gene expression programs governing lineage allocation in hematopoiesis. Enforced expression of GFI1B in K562 erythroleukemia cells favors erythroid over megakaryocytic differentiation, providing a platform to define molecular determinants of binary fate decisions triggered by GFI1B. We deployed proteome-wide proximity labeling to identify factors whose inclusion in GFI1B complexes depends upon GFI1B's obligate effector, Lysine Specific Demethylase (LSD)1. Read More

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http://dx.doi.org/10.1128/MCB.00020-19DOI Listing
April 2019
1 Read

CKS1 Germ Line Exclusion is Essential for the Transition from Meiosis to Early Embryonic Development.

Mol Cell Biol 2019 Apr 15. Epub 2019 Apr 15.

Tumor Initiation and Maintenance Program, Sanford | Burnham | Prebys Medical Discovery Institute, La Jolla, USA

CKS proteins bind cyclin-dependent kinases (CDKs) and play important roles in cell division control and development, though their precise molecular functions are not fully understood. Mammals express two closely related paralogs called CKS1 and CKS2, but only CKS2 is expressed in the germ line indicating it is solely responsible for regulating CDK functions in meiosis. Using knockout mice, we show that CKS2 is a crucial regulator of MPF (CDK1-Cyclin A/B) activity in meiosis. Read More

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http://dx.doi.org/10.1128/MCB.00590-18DOI Listing

Unexpected Role of Matrix Gla Protein in Osteoclasts: Inhibiting Osteoclast Differentiation and Bone Resorption.

Mol Cell Biol 2019 Apr 15. Epub 2019 Apr 15.

Center for Translational Medicine, The First Affiliated Hospital of Xi'an Jiaotong University. Xi'an, Shaanxi 710061, People's Republic of China.

Matrix Gla-protein (MGP) is an extracellular protein responsible for inhibiting mineralization. MGP inhibits osteoblast mineralization and bone formation by regulating the deposition of bone matrix, However, mice display an osteopenic phenotype. To explain this contradiction, we investigated the role of MGP in osteoclastogenesis, the other side of bone remodeling. Read More

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http://dx.doi.org/10.1128/MCB.00012-19DOI Listing
April 2019
1 Read

Uncoupling the senescence-associated secretory phenotype from cell cycle exit via IL-1 inactivation unveils its pro-tumorigenic role.

Mol Cell Biol 2019 Apr 15. Epub 2019 Apr 15.

Department of Biochemistry and Molecular Pharmacology, Deparment of Urology, NYU Cancer Institute, New York University School of Medicine, New York, NY, USA

Cellular senescence has emerged as a potent tumor-suppressor mechanism in numerous human neoplasias. Senescent cells secrete a distinct set of factors collectively termed the senescence-associated secretory phenotype (SASP), which has been postulated to carry both pro- and anti-tumorigenic properties depending on tissue context. However, the effect of the SASP is poorly understood due to the difficulty of studying the SASP independently of other senescence-associated phenotypes. Read More

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http://dx.doi.org/10.1128/MCB.00586-18DOI Listing

Knockdown of lncRNA H19 represses the progress of pulmonary fibrosis through the TGF-β/Smad3 pathway by regulating miR-140.

Mol Cell Biol 2019 Apr 15. Epub 2019 Apr 15.

Department of Respiration, The First Affiliated Hospital of Zhengzhou University, Zhengzhou.

Long non-coding RNAs (lncRNAs) are involved in various human diseases. Recently, H19 is reported to be upregulated in fibrotic rat lung and play a stimulative role in bleomycin (BLM)-induced pulmonary fibrosis in mice. However, its expression in human fibrotic lung tissues and action mechanism remain unclear. Read More

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http://dx.doi.org/10.1128/MCB.00143-19DOI Listing
April 2019
1 Read
4.777 Impact Factor

NOL12 repression induces nucleolar stress-driven cellular senescence and is associated with normative aging.

Mol Cell Biol 2019 Apr 15. Epub 2019 Apr 15.

i3S - Instituto de Investigação e Inovação em Saúde, Universidade do Porto, Portugal

The nucleolus is a subnuclear compartment with key roles in rRNA synthesis and ribosome biogenesis, complex processes that require hundreds of proteins and factors. Alterations in nucleolar morphology and protein content have been linked to the control of cell proliferation and stress responses, and recently further implicated in cell senescence and ageing. In this study, we report the functional role of NOL12 in the nucleolar homeostasis of human primary fibroblasts. Read More

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http://dx.doi.org/10.1128/MCB.00099-19DOI Listing

Transcriptional suppression of CPI-17 gene expression in vascular smooth muscle cells by TNF, KLF4, and Sp1 is associated with LPS-induced vascular hypocontractility, hypotension, and mortality.

Mol Cell Biol 2019 Apr 1. Epub 2019 Apr 1.

Saha Cardiovascular Research Center, University of Kentucky, Lexington, Kentucky, USA

Vasodilatory shock in sepsis is caused by the failure of the vasculature to respond to vasopressors, which results in hypotension, multi-organ failure, and ultimately patient death. Recently, it was reported that CPI-17, a key player in the regulation of smooth muscle contraction, was downregulated by lipopolysaccharide (LPS) in mesenteric arteries concordant with vascular hypocontractilty. While Sp1 has been shown to activate CPI-17 transcription, it is unknown whether Sp1 is involved in LPS-induced smooth muscle CPI-17 downregulation. Read More

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http://mcb.asm.org/lookup/doi/10.1128/MCB.00070-19
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http://dx.doi.org/10.1128/MCB.00070-19DOI Listing
April 2019
2 Reads
4.777 Impact Factor

Transcriptional and Epigenomic Regulation of Adipogenesis.

Mol Cell Biol 2019 Apr 1. Epub 2019 Apr 1.

Adipocyte Biology and Gene Regulation Section, Laboratory of Endocrinology and Receptor Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892, USA

Understanding adipogenesis, the process of adipocyte development, may provide new ways to treat obesity and related metabolic diseases. Adipogenesis is controlled by coordinated actions of lineage-determining transcription factors and epigenomic regulators. PPARγ and C/EBPα are master adipogenic transcription factors. Read More

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http://dx.doi.org/10.1128/MCB.00601-18DOI Listing
April 2019
1 Read

Isoginkgetin, a natural biflavonoid proteasome inhibitor, sensitizes cancer cells to apoptosis via disruption of lysosomal homeostasis and impaired protein clearance.

Mol Cell Biol 2019 Mar 25. Epub 2019 Mar 25.

Department of Laboratory Medicine and Pathobiology, University of Toronto, Toronto, Canada.

Protein degradation pathways are critical for maintaining proper protein dynamics within the cell, and considerable efforts have been made towards the development of therapeutics targeting these catabolic processes. We report here that isoginkgetin - a naturally derived biflavonoid, sensitized cells undergoing nutrient starvation to apoptosis, induced lysosomal stress, and activated the lysosome biogenesis gene TFEB. Isoginkgetin treatment led to the accumulation of aggregates of poly-ubiquitinated proteins that colocalized strongly with the adaptor protein p62, the 20S proteasome, and the ERAD protein UFD1L. Read More

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http://dx.doi.org/10.1128/MCB.00489-18DOI Listing

Targeted Degradation of Glucose Transporters Protects Against Arsenic Toxicity.

Mol Cell Biol 2019 Mar 18. Epub 2019 Mar 18.

Department of Pathology, Brigham and Women's Hospital and Harvard Medical School, Boston, Massachusetts, United States of America

The abundance of cell surface glucose transporters must be precisely regulated to ensure optimal growth under constantly changing environmental conditions. We recently conducted a proteomic analysis of the cellular response to trivalent arsenic, a ubiquitous environmental toxin and carcinogen. A surprising finding was that a subset of glucose transporters were among the most downregulated proteins in the cell upon arsenic exposure. Read More

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http://dx.doi.org/10.1128/MCB.00559-18DOI Listing
March 2019
1 Read

Rescuing Replication from Barriers: Mechanistic Insights from Single-molecule Studies.

Authors:
Bo Sun

Mol Cell Biol 2019 Mar 18. Epub 2019 Mar 18.

School of Life Science and Technology, ShanghaiTech University, Shanghai 201210, China

To prevent replication failure due to fork barriers, several mechanisms have evolved to restart arrested forks independent of the origin of replication. Our understanding of these mechanisms that underlie replication reactivation has been aided through unique dynamic perspectives offered by single-molecule techniques. These techniques, such as optical tweezers, magnetic tweezers, and fluorescence-based methods, allow researchers to monitor the unwinding of DNA by helicase, nucleotide incorporation during polymerase synthesis and replication fork progression in real time. Read More

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http://mcb.asm.org/lookup/doi/10.1128/MCB.00576-18
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http://dx.doi.org/10.1128/MCB.00576-18DOI Listing
March 2019
5 Reads

Characterization of Mice with a Platelet-Specific Deletion of the Adapter Molecule ADAP.

Mol Cell Biol 2019 May 16;39(9). Epub 2019 Apr 16.

Otto von Guericke University Magdeburg, Institute of Molecular and Clinical Immunology, Magdeburg, Germany

The adhesion and degranulation-promoting adapter protein (ADAP) is expressed in T cells, NK cells, myeloid cells, and platelets. The involvement of ADAP in the regulation of receptor-mediated inside-out signaling leading to integrin activation is well characterized, especially in T cells and in platelets. Due to the fact that animal studies using conventional knockout mice are limited by the overlapping effects of the different ADAP-expressing cells, we generated conditional ADAP knockout mice (ADAP PF4-Cre) (PF4, platelet factor 4). Read More

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http://mcb.asm.org/lookup/doi/10.1128/MCB.00365-18
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http://dx.doi.org/10.1128/MCB.00365-18DOI Listing
May 2019
6 Reads

Correction for A.P. and Laishram, "Nuclear Phosphatidylinositol-Phosphate Type I Kinase α-Coupled Star-PAP Polyadenylation Regulates Cell Invasion".

Mol Cell Biol 2019 Mar 1;39(6). Epub 2019 Mar 1.

Cardiovascular Diseases & Diabetes Biology Group, Rajiv Gandhi Centre for Biotechnology, Trivandrum, India.

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http://dx.doi.org/10.1128/MCB.00025-19DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6399669PMC
March 2019
2 Reads

Inactivation of Cyclic AMP Response Element Transcription Caused by Constitutive p38 Activation Is Mediated by Hyperphosphorylation-Dependent CRTC2 Nucleocytoplasmic Transport.

Mol Cell Biol 2019 May 16;39(9). Epub 2019 Apr 16.

State Key Laboratory of Cellular Stress Biology, Innovation Center for Cell Signaling Network, School of Life Sciences, Xiamen University, Xiamen, Fujian, China

The p38 signal transduction pathway can be activated transiently or constitutively, depending on the contexts in which the activation occurs. However, the biological consequence of constitutive activation of p38 is largely unknown. After screening 300 transcriptional cofactors, we identified CRTC2 as a downstream substrate of constitutively activated p38. Read More

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http://mcb.asm.org/lookup/doi/10.1128/MCB.00554-18
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http://dx.doi.org/10.1128/MCB.00554-18DOI Listing
May 2019
1 Read

Cell Cycle Progression Regulates Biogenesis and Cellular Localization of Lipid Droplets.

Mol Cell Biol 2019 May 16;39(9). Epub 2019 Apr 16.

Program of Immunology and Tumor Biology, Brazilian National Cancer Institute (INCA), Rio de Janeiro, RJ, Brazil

Intracellular lipid accumulation has been associated with a poor prognosis in cancer. We have previously reported the involvement of lipid droplets in cell proliferation in colon cancer cells, suggesting a role for these organelles in cancer development. In this study, we evaluate the role of lipid droplets in cell cycle regulation and cellular transformation. Read More

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http://dx.doi.org/10.1128/MCB.00374-18DOI Listing
May 2019
1 Read

The Molecular Chaperone Heat Shock Protein 70 Controls Liver Cancer Initiation and Progression by Regulating Adaptive DNA Damage and Mitogen-Activated Protein Kinase/Extracellular Signal-Regulated Kinase Signaling Pathways.

Mol Cell Biol 2019 May 16;39(9). Epub 2019 Apr 16.

Molecular Chaperone Biology, Medical College of Georgia, Augusta University, Augusta, Georgia, USA

Delineating the mechanisms that drive hepatic injury and hepatocellular carcinoma (HCC) progression is critical for development of novel treatments for recurrent and advanced HCC but also for the development of diagnostic and preventive strategies. Heat shock protein 70 (HSP70) acts in concert with several cochaperones and nucleotide exchange factors and plays an essential role in protein quality control that increases survival by protecting cells against environmental stressors. Specifically, the HSP70-mediated response has been implicated in the pathogenesis of cancer, but the specific mechanisms by which HSP70 may support malignant cell transformation remains to be fully elucidated. Read More

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http://dx.doi.org/10.1128/MCB.00391-18DOI Listing
May 2019
1 Read

Distinct Functions of the Cap-Binding Complex in Stimulation of Nuclear mRNA Export.

Mol Cell Biol 2019 Apr 2;39(8). Epub 2019 Apr 2.

Department of Biochemistry and Molecular Biology, Southern Illinois University School of Medicine, Carbondale, Illinois, USA

Cap-binding complex (CBC) associates cotranscriptionally with the cap structure at the 5' end of nascent mRNA to protect it from exonucleolytic degradation. Here, we show that CBC promotes the targeting of an mRNA export adaptor, Yra1 (forming transcription export [TREX] complex with THO and Sub2), to the active genes and enhances mRNA export in Likewise, recruitment of Npl3 (an hnRNP involved in mRNA export via formation of export-competent ribonuclear protein complex [RNP]) to the active genes is facilitated by CBC. Thus, CBC enhances targeting of the export factors and promotes mRNA export. Read More

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http://dx.doi.org/10.1128/MCB.00540-18DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6447411PMC

tRNA Genes Affect Chromosome Structure and Function via Local Effects.

Mol Cell Biol 2019 Apr 2;39(8). Epub 2019 Apr 2.

Department of MCD Biology, University of California, Santa Cruz, California, USA

The genome is packaged and organized in an ordered, nonrandom manner, and specific chromatin segments contact nuclear substructures to mediate this organization. tRNA genes (tDNAs) are binding sites for transcription factors and architectural proteins and are thought to play an important role in the organization of the genome. In this study, we investigate the roles of tDNAs in genomic organization and chromosome function by editing a chromosome so that it lacked any tDNAs. Read More

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http://dx.doi.org/10.1128/MCB.00432-18DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6447413PMC
April 2019
2 Reads

Zfp423 Regulates Skeletal Muscle Regeneration and Proliferation.

Mol Cell Biol 2019 Apr 2;39(8). Epub 2019 Apr 2.

Department of Medicine, Harvard Medical School, Boston, Massachusetts, USA

Satellite cells (SCs) are skeletal muscle stem cells that proliferate in response to injury and provide myogenic precursors for growth and repair. Zfp423 is a transcriptional cofactor expressed in multiple immature cell populations, such as neuronal precursors, mesenchymal stem cells, and preadipocytes, where it regulates lineage allocation, proliferation, and differentiation. Here, we show that Zfp423 regulates myogenic progression during muscle regeneration. Read More

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http://dx.doi.org/10.1128/MCB.00447-18DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6447414PMC
April 2019
7 Reads

Reactive Oxygen Species Signaling Promotes Hypoxia-Inducible Factor 1α Stabilization in Sonic Hedgehog-Driven Cerebellar Progenitor Cell Proliferation.

Mol Cell Biol 2019 Apr 2;39(8). Epub 2019 Apr 2.

Department of Pediatric Oncology, Emory University School of Medicine, Atlanta, Georgia, USA

Cerebellar development is a highly regulated process involving numerous factors acting with high specificity, both temporally and by location. Part of this process involves extensive proliferation of cerebellar granule neuron precursors (CGNPs) induced by Sonic Hedgehog (SHH) signaling, but downstream effectors of mitogenic signaling are still being elucidated. Using primary CGNP cultures, a well-established model for SHH-driven proliferation, we show that SHH-treated CGNPs feature high levels of hypoxia-inducible factor 1α (HIF1α), which is known to promote glycolysis, stemness, and angiogenesis. Read More

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http://dx.doi.org/10.1128/MCB.00268-18DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6447416PMC
April 2019
1 Read

E3 Ubiquitin Ligases RNF20 and RNF40 Are Required for Double-Stranded Break (DSB) Repair: Evidence for Monoubiquitination of Histone H2B Lysine 120 as a Novel Axis of DSB Signaling and Repair.

Mol Cell Biol 2019 Apr 2;39(8). Epub 2019 Apr 2.

Department of Immunology, University of Toronto, Toronto, Ontario, Canada

Histone posttranslational modifications play fundamental roles in the regulation of double-stranded DNA break (DSB) repair. RNF20/RNF40-mediated monoubiquitination of histone H2B on lysine 120 (H2Bub) has been suggested as a potential mediator of DSB repair, although the nature and function of this posttranslational modification remain enigmatic. In this report, we demonstrate that RNF20 and RNF40 are required for DSB repair leading to homologous recombination (HR) and class switch recombination, a process driven by nonhomologous end joining (NHEJ), in mouse B cells. Read More

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http://dx.doi.org/10.1128/MCB.00488-18DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6447412PMC
April 2019
1 Read

Dynamic Methylation of an L1 Transduction Family during Reprogramming and Neurodifferentiation.

Mol Cell Biol 2019 Apr 19;39(7). Epub 2019 Mar 19.

Mater Research Institute, University of Queensland, Woolloongabba, Queensland, Australia

The retrotransposon LINE-1 (L1) is a significant source of endogenous mutagenesis in humans. In each individual genome, a few retrotransposition-competent L1s (RC-L1s) can generate new heritable L1 insertions in the early embryo, primordial germ line, and germ cells. L1 retrotransposition can also occur in the neuronal lineage and cause somatic mosaicism. Read More

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http://mcb.asm.org/lookup/doi/10.1128/MCB.00499-18
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http://dx.doi.org/10.1128/MCB.00499-18DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6425141PMC
April 2019
5 Reads

IMP2 Increases Mouse Skeletal Muscle Mass and Voluntary Activity by Enhancing Autocrine Insulin-Like Growth Factor 2 Production and Optimizing Muscle Metabolism.

Mol Cell Biol 2019 Apr 19;39(7). Epub 2019 Mar 19.

Department of Molecular Biology, Massachusetts General Hospital, Boston, Massachusetts, USA

Insulin-like growth factor 2 (IGF2) mRNA binding protein 2 (IMP2) was selectively deleted from adult mouse muscle; two phenotypes were observed: decreased accrual of skeletal muscle mass after weaning and reduced wheel-running activity but normal forced treadmill performance. Reduced wheel running occurs when mice are fed a high-fat diet but is normalized when mice consume standard chow. The two phenotypes are due to altered output from different IMP2 client mRNAs. Read More

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http://dx.doi.org/10.1128/MCB.00528-18DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6425142PMC
April 2019
1 Read

Generation and Molecular Characterization of Human Ring Sideroblasts: a Key Role of Ferrous Iron in Terminal Erythroid Differentiation and Ring Sideroblast Formation.

Mol Cell Biol 2019 Apr 19;39(7). Epub 2019 Mar 19.

Department of Hematology and Rheumatology, Tohoku University Graduate School of Medicine, Sendai, Japan

Ring sideroblasts are a hallmark of sideroblastic anemia, although little is known about their characteristics. Here, we first generated mutant mice by disrupting the GATA-1 binding motif at the intron 1 enhancer of the gene, a gene responsible for X-linked sideroblastic anemia (XLSA). Although heterozygous female mice showed an anemic phenotype, ring sideroblasts were not observed in their bone marrow. Read More

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http://mcb.asm.org/lookup/doi/10.1128/MCB.00387-18
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http://dx.doi.org/10.1128/MCB.00387-18DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6425143PMC
April 2019
18 Reads

Increased Expression of MicroRNA 551a by c-Fos Reduces Focal Adhesion Kinase Levels and Blocks Tumorigenesis.

Mol Cell Biol 2019 Apr 19;39(7). Epub 2019 Mar 19.

Department of Biotechnology, Indian Institute of Technology Madras, Chennai, India

Breast cancer is a recurrent type of cancer among women worldwide. Despite remarkable progress in the prevention, detection, and treatment of breast cancer, it still remains a major chronic problem worldwide and poses significant challenges, like metastasis to distant organs, demanding the need for novel biomarkers and therapeutic targets. Focal adhesion kinase (FAK), a member of the protein tyrosine kinases, has been shown to be expressed in high levels in breast tumors. Read More

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http://mcb.asm.org/lookup/doi/10.1128/MCB.00577-18
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http://dx.doi.org/10.1128/MCB.00577-18DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6425145PMC
April 2019
11 Reads

Mediator Subunit Med1 Is Required for GATA-Dependent Developmental Processes: Divergent Binding Interfaces for Conserved Coactivator Functions.

Mol Cell Biol 2019 Apr 19;39(7). Epub 2019 Mar 19.

Centre de Biologie Intégrative (CBI)-CBD, UMR5547 CNRS/Université Toulouse III, Toulouse, France

DNA-bound transcription factors (TFs) governing developmental gene regulation have been proposed to recruit polymerase II machinery at gene promoters through specific interactions with dedicated subunits of the evolutionarily conserved Mediator (MED) complex. However, whether such MED subunit-specific functions and partnerships have been conserved during evolution has been poorly investigated. To address this issue, we generated the first loss-of-function mutants for Med1, known as a specific cofactor for GATA TFs and hormone nuclear receptors in mammals. Read More

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http://dx.doi.org/10.1128/MCB.00477-18DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6425144PMC

Reevaluation of the Role of Extracellular Signal-Regulated Kinase 3 in Perinatal Survival and Postnatal Growth Using New Genetically Engineered Mouse Models.

Mol Cell Biol 2019 Mar 1;39(6). Epub 2019 Mar 1.

Institute for Research in Immunology and Cancer, Université de Montréal, Montreal, Quebec, Canada

The physiological functions of the atypical mitogen-activated protein kinase extracellular signal-regulated kinase 3 (ERK3) remain poorly characterized. Previous analysis of mice with a targeted insertion of the reporter in the locus ( ) showed that inactivation of ERK3 in mice leads to perinatal lethality associated with intrauterine growth restriction, defective lung maturation, and neuromuscular anomalies. To further explore the role of ERK3 in physiology and disease, we generated novel mouse models expressing a catalytically inactive ( ) or conditional ( ) allele of ERK3. Read More

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http://dx.doi.org/10.1128/MCB.00527-18DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6399666PMC
March 2019
1 Read

Germ Line Deletion Reveals a Nonessential Role of Atypical Mitogen-Activated Protein Kinase 6/Extracellular Signal-Regulated Kinase 3.

Mol Cell Biol 2019 Mar 1;39(6). Epub 2019 Mar 1.

Institute of Cell Biochemistry, Hannover Medical School, Hannover, Germany

Mitogen-activated protein kinase 6/extracellular signal-regulated kinase 3 (MAPK6/ERK3) is an atypical member of the MAPKs. An essential role has been suggested by the perinatal lethal phenotype of ERK3 knockout mice carrying a insertion in exon 2 due to pulmonary dysfunction and by defects in function, activation, and positive selection of T cells. To study the role of ERK3 , we generated mice carrying a conditional allele with exon 3 flanked by sites. Read More

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http://dx.doi.org/10.1128/MCB.00516-18DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6399663PMC
March 2019
2 Reads

A Feedback Loop between MicroRNA 155 (miR-155), Programmed Cell Death 4, and Activation Protein 1 Modulates the Expression of miR-155 and Tumorigenesis in Tongue Cancer.

Mol Cell Biol 2019 Mar 1;39(6). Epub 2019 Mar 1.

Department of Biotechnology, Bhupat and Jyoti Mehta School of Biosciences, Indian Institute of Technology Madras, Chennai, India

MicroRNA 155 (miR-155) is an oncomir, generated as a noncoding RNA from the gene whose promoter activity is mainly controlled via activation protein 1 (AP-1) and NF-κB transcription factors. We found that the expression levels of miR-155 and programmed cell death 4 (Pdcd4) exhibit inverse relationships in tongue cancer cells (SAS and AWL) and tumor tissues compared to their relationships in normal FBM cells and normal tongue tissues, respectively. and studies with the 3' untranslated region (UTR) of Pdcd4 via luciferase reporter assays, quantitative PCR (qPCR), and Western blotting showed that miR-155 directly targets Pdcd4 mRNA and blocks its expression. Read More

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http://mcb.asm.org/lookup/doi/10.1128/MCB.00410-18
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http://dx.doi.org/10.1128/MCB.00410-18DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6399668PMC
March 2019
13 Reads

Loss of Estrogen-Related Receptor Alpha Facilitates Angiogenesis in Endothelial Cells.

Mol Cell Biol 2019 Mar 15;39(5). Epub 2019 Feb 15.

Metabolic and Degenerative Diseases, Institute of Molecular Medicine, The University of Texas McGovern Medical School, Houston, Texas, USA

Estrogen-related receptors (ERRs) have emerged as major metabolic regulators in various tissues. However, their expression and function in the vasculature remains unknown. Here, we report the transcriptional program and cellular function of ERRα in endothelial cells (ECs), a cell type with a multifaceted role in vasculature. Read More

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http://dx.doi.org/10.1128/MCB.00411-18DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6379583PMC
March 2019
3 Reads

CSB-Dependent Cyclin-Dependent Kinase 9 Degradation and RNA Polymerase II Phosphorylation during Transcription-Coupled Repair.

Mol Cell Biol 2019 Mar 1;39(6). Epub 2019 Mar 1.

Institut NeuroMyoGène (INMG), CNRS UMR 5310, INSERM U1217, Université de Lyon, Université Claude Bernard Lyon 1, Villeurbanne, France

DNA lesions block cellular processes such as transcription, inducing apoptosis, tissue failures, and premature aging. To counteract the deleterious effects of DNA damage, cells are equipped with various DNA repair pathways. Transcription-coupled repair specifically removes helix-distorting DNA adducts in a coordinated multistep process. Read More

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http://dx.doi.org/10.1128/MCB.00225-18DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6399667PMC

Common and Differential Transcriptional Actions of Nuclear Receptors Liver X Receptors α and β in Macrophages.

Mol Cell Biol 2019 Mar 15;39(5). Epub 2019 Feb 15.

Instituto de Investigaciones Biomédicas Alberto Sols, Consejo Superior de Investigaciones Científicas (CSIC)-Universidad Autónoma de Madrid, Madrid, Spain

The liver X receptors α and β (LXRα and LXRβ) are oxysterol-activated transcription factors that coordinately regulate gene expression that is important for cholesterol and fatty acid metabolism. In addition to their roles in lipid metabolism, LXRs participate in the transcriptional regulation of macrophage activation and are considered potent regulators of inflammation. LXRs are highly similar, and despite notable exceptions, most of their reported functions are substantially overlapping. Read More

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http://dx.doi.org/10.1128/MCB.00376-18DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6379585PMC
March 2019
1 Read

RNA Binding Protein HuR Promotes Autophagosome Formation by Regulating Expression of Autophagy-Related Proteins 5, 12, and 16 in Human Hepatocellular Carcinoma Cells.

Mol Cell Biol 2019 Mar 1;39(6). Epub 2019 Mar 1.

Department of Biochemistry, The Catholic University of Korea College of Medicine, Seoul, South Korea

Autophagy is a process of lysosomal self-degradation of cellular components by forming autophagosomes. Autophagosome formation is an essential process in autophagy and is fine-tuned by various autophagy-related gene (ATG) products, including ATG5, ATG12, and ATG16. Although several reports have shown that numerous factors affect multiple levels of gene regulation to orchestrate cellular autophagy, the detailed mechanism of autophagosome formation still needs further investigation. Read More

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http://dx.doi.org/10.1128/MCB.00508-18DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6399664PMC
March 2019
5 Reads

Rapid Recapitulation of Nonalcoholic Steatohepatitis upon Loss of Host Cell Factor 1 Function in Mouse Hepatocytes

Mol Cell Biol 2019 02 15;39(5). Epub 2019 Feb 15.

Center for Integrative Genomics, Génopode, University of Lausanne, Lausanne, Switzerland

Host-cell factor 1 (HCF-1), encoded by the ubiquitously expressed X-linked gene , is an epigenetic coregulator important for mouse development and cell proliferation, including during liver regeneration. We used a hepatocyte-specific inducible knock-out allele (called ), to induce HCF-1 loss in hepatocytes of hemizygous males by four days. In heterozygous females, owing to random X-chromosome inactivation, upon allele induction, a 50/50 mix of HCF-1 positive and negative hepatocyte clusters is engineered. Read More

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http://mcb.asm.org/lookup/doi/10.1128/MCB.00405-18
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http://dx.doi.org/10.1128/MCB.00405-18DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6379584PMC
February 2019
16 Reads

G-Alpha Subunit Abundance and Activity Differentially Regulate β-Catenin Signaling.

Mol Cell Biol 2019 Mar 15;39(5). Epub 2019 Feb 15.

Centre for Craniofacial and Regenerative Biology, King's College London, Guy's Hospital, London, United Kingdom

Heterotrimeric G proteins are signal transduction proteins involved in regulating numerous signaling events. In particular, previous studies have demonstrated a role for G-proteins in regulating β-catenin signaling. However, the link between G-proteins and β-catenin signaling is controversial and appears to depend on G-protein specificity. Read More

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http://dx.doi.org/10.1128/MCB.00422-18DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6379582PMC

Role of SMPD3 during Bone Fracture Healing and Regulation of Its Expression.

Mol Cell Biol 2019 Feb 4;39(4). Epub 2019 Feb 4.

Faculty of Dentistry, McGill University, Montreal, Quebec, Canada

Sphingomyelin phosphodiesterase 3 (SMPD3), a lipid-metabolizing enzyme present in bone and cartilage, has important roles in the developing skeleton. We previously showed that SMPD3 deficiency results in delayed extracellular matrix (ECM) mineralization and severe skeletal deformities in an inducible knockout mouse model, ; mice, in which was ablated in -expressing chondrocytes and osteoblasts during early skeletogenesis. However, as shown in the current study, ablation of postnatally in 3-month-old ; mice resulted in only a mild bone mineralization defect. Read More

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http://dx.doi.org/10.1128/MCB.00370-18DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6362318PMC
February 2019

Yeast Mpo1 Is a Novel Dioxygenase That Catalyzes the α-Oxidation of a 2-Hydroxy Fatty Acid in an Fe-Dependent Manner.

Mol Cell Biol 2019 Mar 15;39(5). Epub 2019 Feb 15.

Laboratory of Biochemistry, Faculty of Pharmaceutical Sciences, Hokkaido University, Sapporo, Japan

Phytosphingosine (PHS) is the major long-chain base component of sphingolipids in The PHS metabolic pathway includes a fatty acid (FA) α-oxidation reaction. Recently, we identified the novel protein Mpo1, which is involved in PHS metabolism. However, the details of the FA α-oxidation reaction and the role of Mpo1 in PHS metabolism remained unclear. Read More

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http://mcb.asm.org/lookup/doi/10.1128/MCB.00428-18
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http://dx.doi.org/10.1128/MCB.00428-18DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6379581PMC
March 2019
7 Reads

Both a Unique Motif at the C Terminus and an N-Terminal HEAT Repeat Contribute to G-Quadruplex Binding and Origin Regulation by the Rif1 Protein.

Mol Cell Biol 2019 Feb 4;39(4). Epub 2019 Feb 4.

Department of Genome Medicine, Tokyo Metropolitan Institute of Medical Science, Kamikitazawa, Setagaya-ku, Tokyo, Japan

Rif1 is a key factor for spatiotemporal regulation of DNA replication. Rif1 suppresses origin firing in the mid-late replication domains by generating replication-suppressive chromatin architecture and by recruiting a protein phosphatase. In fission yeast, the function of Hsk1, a kinase important for origin firing, can be bypassed by Δ due to the loss of origin suppression. Read More

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http://mcb.asm.org/lookup/doi/10.1128/MCB.00364-18
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http://dx.doi.org/10.1128/MCB.00364-18DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6362314PMC
February 2019
37 Reads

hnRNP Q Regulates Internal Ribosome Entry Site-Mediated Translation in Neurons.

Mol Cell Biol 2019 Feb 4;39(4). Epub 2019 Feb 4.

Department of Life Sciences, Pohang University of Science and Technology, Pohang, Republic of Korea

Fragile X syndrome (FXS) caused by loss of fragile X mental retardation protein (FMRP), is the most common cause of inherited intellectual disability. Numerous studies show that FMRP is an RNA binding protein that regulates translation of its binding targets and plays key roles in neuronal functions. However, the regulatory mechanism for FMRP expression is incompletely understood. Read More

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http://dx.doi.org/10.1128/MCB.00371-18DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6362317PMC
February 2019
2 Reads

Mutant p53 Sequestration of the MDM2 Acidic Domain Inhibits E3 Ligase Activity.

Mol Cell Biol 2019 Feb 4;39(4). Epub 2019 Feb 4.

Molecular Oncology Department, Moffitt Cancer Center, Tampa, Florida, USA

Missense p53 mutants often accumulate in tumors and drive progression through gain of function. MDM2 efficiently degrades wild-type p53 but fails to degrade mutant p53 in tumor cells. Previous studies revealed that mutant p53 inhibits MDM2 autoubiquitination, suggesting that the interaction inhibits MDM2 E3 activity. Read More

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http://dx.doi.org/10.1128/MCB.00375-18DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6362316PMC
February 2019

A Novel Regulatory Axis, CHD1L-MicroRNA 486-Matrix Metalloproteinase 2, Controls Spermatogonial Stem Cell Properties.

Mol Cell Biol 2019 Feb 4;39(4). Epub 2019 Feb 4.

Affiliated Cancer Hospital & Institute of Guangzhou Medical University, Xinzao Town, Panyu District, Guangzhou, Guangdong, China

Spermatogonial stem cells (SSCs) are unipotent germ cells that are at the foundation of spermatogenesis and male fertility. However, the underlying molecular mechanisms governing SSC stemness and growth properties remain elusive. We have recently identified chromodomain helicase/ATPase DNA binding protein 1-like (Chd1l) as a novel regulator for SSC survival and self-renewal, but how these functions are controlled by Chd1l remains to be resolved. Read More

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http://mcb.asm.org/lookup/doi/10.1128/MCB.00357-18
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http://dx.doi.org/10.1128/MCB.00357-18DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6362313PMC
February 2019
28 Reads

The Phosphorylated Estrogen Receptor α (ER) Cistrome Identifies a Subset of Active Enhancers Enriched for Direct ER-DNA Binding and the Transcription Factor GRHL2.

Mol Cell Biol 2019 Feb 16;39(3). Epub 2019 Jan 16.

McArdle Laboratory for Cancer Research, Department of Oncology and Carbone Comprehensive Cancer Center, University of Wisconsin-Madison, Madison, Wisconsin, USA

Posttranslational modifications are key regulators of protein function, providing cues that can alter protein interactions and cellular location. Phosphorylation of estrogen receptor α (ER) at serine 118 (pS118-ER) occurs in response to multiple stimuli and is involved in modulating ER-dependent gene transcription. While the cistrome of ER is well established, surprisingly little is understood about how phosphorylation impacts ER-DNA binding activity. Read More

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http://dx.doi.org/10.1128/MCB.00417-18DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6336141PMC
February 2019
14 Reads

Cancer-Associated Eukaryotic Translation Initiation Factor 1A Mutants Impair Rps3 and Rps10 Binding and Enhance Scanning of Cell Cycle Genes.

Mol Cell Biol 2019 Feb 16;39(3). Epub 2019 Jan 16.

Department of Biomolecular Sciences, The Weizmann Institute of Science, Rehovot, Israel

Protein synthesis is linked to cell proliferation, and its deregulation contributes to cancer. Eukaryotic translation initiation factor 1A (eIF1A) plays a key role in scanning and AUG selection and differentially affects the translation of distinct mRNAs. Its unstructured N-terminal tail (NTT) is frequently mutated in several malignancies. Read More

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http://mcb.asm.org/lookup/doi/10.1128/MCB.00441-18
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http://dx.doi.org/10.1128/MCB.00441-18DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6336143PMC
February 2019
5 Reads

Nuclear Phosphatidylinositol 5-Phosphatase Is Essential for Allelic Exclusion of Variant Surface Glycoprotein Genes in Trypanosomes.

Mol Cell Biol 2019 Feb 16;39(3). Epub 2019 Jan 16.

Center for Infectious Disease Research, Seattle, Washington, USA

Allelic exclusion of variant surface glycoprotein (VSG) genes is essential for African trypanosomes to evade the host antibody response by antigenic variation. The mechanisms by which this parasite expresses only one of its ∼2,000 VSG genes at a time are unknown. We show that nuclear phosphatidylinositol 5-phosphatase (PIP5Pase) interacts with repressor activator protein 1 (RAP1) in a multiprotein complex and functions in the control of VSG allelic exclusion. Read More

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http://dx.doi.org/10.1128/MCB.00395-18DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6336139PMC
February 2019
1 Read

Senescent Breast Luminal Cells Promote Carcinogenesis through Interleukin-8-Dependent Activation of Stromal Fibroblasts.

Mol Cell Biol 2019 Jan 3;39(2). Epub 2019 Jan 3.

Department of Molecular Oncology, King Faisal Specialist Hospital and Research Centre, Riyadh, Kingdom of Saudi Arabia

Aging and stress promote senescence, which has intrinsic tumor suppressor functions and extrinsic tumor promoting properties. Therefore, it is of utmost importance to delineate the effects of senescence inducers on the various types of cells that compose the different organs. We show here that primary normal breast luminal (NBL) cells are more sensitive than their corresponding stromal fibroblasts to proliferative as well as oxidative damage-induced senescence. Read More

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http://mcb.asm.org/lookup/doi/10.1128/MCB.00359-18
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http://dx.doi.org/10.1128/MCB.00359-18DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6321881PMC
January 2019
27 Reads
4.780 Impact Factor

Coordinated Regulation of Intracellular Fascin Distribution Governs Tumor Microvesicle Release and Invasive Cell Capacity.

Mol Cell Biol 2019 Feb 16;39(3). Epub 2019 Jan 16.

Department of Biological Sciences, University of Notre Dame, Notre Dame, Indiana, USA

Tumor cell invasion is one result of the bidirectional interactions occurring between tumor cells and the surrounding milieu. The ability of tumor cells to invade through the extracellular matrix is in part regulated by the formation of a class of protease-loaded extracellular vesicles, called tumor microvesicles (TMVs), which are released directly from the cell surface. Here we show that the actin bundling protein, fascin, redistributes to the cell periphery in a ternary complex with podocalyxin and ezrin, where it promotes TMV release. Read More

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http://mcb.asm.org/lookup/doi/10.1128/MCB.00264-18
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http://dx.doi.org/10.1128/MCB.00264-18DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6336144PMC
February 2019
15 Reads