3,537 results match your criteria Molecular Vision [Journal]


Micromorphology analysis of the anterior human lens capsule.

Mol Vis 2018 31;24:902-912. Epub 2018 Dec 31.

Department of Biomedical Engineering, University of Miami College of Engineering, Coral Gables, FL.

Purpose: This study aimed to quantify the three-dimensional micromorphology of the surface of the human lens capsule as a function of age.

Methods: Imaging experiments were conducted on whole human lenses received from eight human cadavers (donor age range: 30-88 years). Imaging was performed with an atomic force microscope (AFM) in contact mode in fluid. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6334982PMC
December 2018
1 Read

Age-related aqueous humor (AH) and lens epithelial cell/capsule protein carbonylation and AH protein concentration in cataract patients who have pseudoexfoliative diseases.

Mol Vis 2018 31;24:890-901. Epub 2018 Dec 31.

Department of Biology, University of Patras, Patras, Greece.

Purpose: The aim of this study is to investigate the age-correlation of oxidative stress (OS, assessed by the accumulative OS damage marker protein carbonyls) in aqueous humour (AH; together with protein concentration) and lens epithelial cells plus capsule (LECs/capsule) in patients with cataract (CAT), and also suffering from pseudoexfoliation syndrome (PEX), primary open-angle glaucoma (POAG) and pseudoexfoliation glaucoma (PXG).

Methods: AH samples from 78 male/female patients (21, 20, 19 and 18 with CAT, PEX, PXG, and POAG, respectively), and LECs/capsule samples from 104 male/female patients (34, 32, 18, and 20 with CAT, PEX, PXG and POAG, respectively) were collected during phacoemulsification CAT surgery. Average protein carbonyl concentrations were measured in patients grouped in 5-year age intervals (ranging from 56-60 to 86-90). Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6334981PMC
December 2018

Comprehensive characterization of the adult ND4 Swiss Webster mouse retina: Using discovery-based mass spectrometry to decipher the total proteome and phosphoproteome.

Mol Vis 2018 31;24:875-889. Epub 2018 Dec 31.

Department of Ophthalmology, Louisiana State University Health Science Center (LSUHSC), New Orleans, LA.

Purpose: Diverse groups of proteins play integral roles in both the physiology and pathophysiology of the retina. However, thorough proteomic analyses of retinas of experimental species are currently unavailable. The purpose of the present paper is providing the field with a comprehensive proteomic characterization of the retina of a commonly used laboratory mouse using a discovery-based mass spectrometry (MS) approach. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6334985PMC
December 2018

Immunophenotypic characterization of telocyte-like cells in pterygium.

Mol Vis 2018 29;24:853-866. Epub 2018 Dec 29.

Department of Biomedical Sciences, University of Cagliari, Italy.

Purpose: Telocytes (TCs) are peculiar interstitial cells, characterized by their typical elongated and interconnected processes called telopodes. TCs are supposed to contribute to maintain tissue homeostasis but also to be involved in the pathophysiology of many disorders. The aim of the study was to identify TCs in pterygium, a chronic condition of bulbar conjunctiva, and to examine possible differences in TCs in terms of immunophenotype and/or localization between pterygium and normal conjunctiva, to evaluate the possible involvement of TCs in pathogenesis of pterygium. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6334986PMC
December 2018

Identification and functional analysis of a novel oculocerebrorenal syndrome of Lowe () gene variant in two pedigrees with varying phenotypes including isolated congenital cataract.

Mol Vis 2018 31;24:847-852. Epub 2018 Dec 31.

Clinical and Experimental Sciences, University of Southampton, UK.

Purpose: To identify the genetic variation in two unrelated probands with congenital cataract and to perform functional analysis of the detected variants.

Methods: Clinical examination and phenotyping, segregation, and functional analysis were performed for the two studied pedigrees.

Results: A novel gene variant (c. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6334980PMC
December 2018
7 Reads
1.986 Impact Factor

Rescue of cone function in cone-only knockout mouse model with Leber congenital amaurosis phenotype.

Mol Vis 2018 30;24:834-846. Epub 2018 Dec 30.

Department of Ophthalmology, John A. Moran Eye Center, University of Utah Health Science Center, Salt Lake City, UT.

Purpose: Recessive mutations in the human gene are associated with Senior-Løken syndrome (SLS), a ciliopathy presenting with nephronophthisis and Leber congenital amaurosis (LCA). -knockout mice develop LCA without nephronophthisis. Mutant rods rapidly degenerate while mutant cones survive for months. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6334983PMC
December 2018

Bulk changes in posterior scleral collagen microstructure in human high myopia.

Mol Vis 2018 30;24:818-833. Epub 2018 Dec 30.

Structural Biophysics Research Group, School of Optometry and Vision Sciences, Cardiff University, Cardiff, UK.

Purpose: We aimed to characterize any bulk changes in posterior scleral collagen fibril bundle architecture in human eyes with high myopia.

Methods: Wide-angle X-ray scattering (WAXS) was employed to map collagen orientation at 0.5 mm × 0. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6334987PMC
December 2018

Novel protein constituents of pathological ocular pseudoexfoliation syndrome deposits identified with mass spectrometry.

Mol Vis 2018 28;24:801-817. Epub 2018 Dec 28.

Department of Ophthalmology, Flinders University, Bedford Park, SA, Australia.

Purpose: Pseudoexfoliation (PEX) syndrome is an age-related progressive disease of the extracellular matrix with ocular manifestations. PEX is clinically diagnosed by the presence of extracellular exfoliative deposits on the anterior surface of the ocular lens. PEX syndrome is a major risk factor for developing glaucoma, the leading cause of irreversible blindness in the world, and is often associated with the development of cataract. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6334984PMC
December 2018
1 Read

Nintedanib inhibits TGF-β-induced myofibroblast transdifferentiation in human Tenon's fibroblasts.

Mol Vis 2018 9;24:789-800. Epub 2018 Dec 9.

State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou, China.

Purpose: This study aimed to investigate the effect of nintedanib on the conversion of human Tenon's fibroblasts (HTFs) into myofibroblasts and reveal the molecular mechanisms involved.

Methods: Primary cultured HTFs were incubated with transforming growth factor β1 (TGF-β1) alone or combined with nintedanib, and cell proliferation and migration were measured by cell counting kit-8 (CCK8) and the scratch wound assay, respectively. HTF contractility was evaluated with a 3D collagen contraction assay. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6300612PMC
December 2018
1 Read

Spectrum of germline mutations and clinical manifestations in retinoblastoma patients from Thailand.

Mol Vis 2018 9;24:778-788. Epub 2018 Dec 9.

Department of Pediatrics, Faculty of Medicine Ramathibodi Hospital, Mahidol University, Bangkok, Thailand.

Purpose: Retinoblastoma (RB) is a retinal tumor that most commonly occurs in children. Approximately 40% of RB patients carry germline mutations in the gene. RB survivors with germline mutations are at increased risk of passing on the disease to future offspring and of secondary cancer in adulthood. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6300611PMC
December 2018
2 Reads

Intravitreal bevacizumab upregulates transthyretin in experimental branch retinal vein occlusion.

Mol Vis 2018 26;24:759-766. Epub 2018 Nov 26.

Department of Ophthalmology, Aalborg University Hospital, Aalborg, Denmark.

Purpose: To identify retinal protein changes that mediate beneficial effects of intravitreal bevacizumab in experimental branch retinal vein occlusion (BRVO).

Methods: In six Danish Landrace pigs, BRVO was induced with argon laser in both eyes. After BRVO was induced, the right eye of each animal was given an intravitreal injection of bevacizumab while the left eye was treated with saline water. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6279196PMC
November 2018
6 Reads

Echinacoside protects retinal ganglion cells from ischemia/reperfusion-induced injury in the rat retina.

Mol Vis 2018 25;24:746-758. Epub 2018 Nov 25.

Department of Ophthalmology, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.

Objective: To investigate whether echinacoside (ECH) protects the retina against ischemia/reperfusion (I/R) injury and the underlying mechanisms.

Methods: Adult male Wistar rats were randomly divided into four groups: sham, sham plus ECH, I/R plus vehicle, and I/R plus ECH. Before the retinal I/R injury produced by high intraocular pressure (HOP), ECH was administered (20 mg/kg daily) for 7 days. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6279312PMC
November 2018
7 Reads

Photoreceptor preservation induced by intravitreal controlled delivery of GDNF and GDNF/melatonin in rhodopsin knockout mice.

Mol Vis 2018 16;24:733-745. Epub 2018 Nov 16.

Pharmaceutical Innovation in Ophthalmology Research Group, UCM 920415, Department of Pharmaceutics and Food Technology, Faculty of Pharmacy, Complutense University, Madrid, Spain.

Purpose: To evaluate the potential of a poly(lactic-co-glycolic acid) (PLGA)-based slow release formulation of glial cell line-derived neurotrophic factor (GDNF) alone or in combination with melatonin to rescue photoreceptors in a mouse model of retinal degeneration.

Methods: GDNF and GDNF/melatonin-loaded PLGA microspheres (MSs) were prepared using a solid-in-oil-in-water emulsion solvent extraction-evaporation technique. A combination of PLGA and vitamin E (VitE) was used to create the microcarriers. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6279195PMC
November 2018
11 Reads

Epac1 deacetylates HMGB1 through increased IGFBP-3 and SIRT1 levels in the retinal vasculature.

Mol Vis 2018 16;24:727-732. Epub 2018 Nov 16.

Department of Ophthalmology, Visual and Anatomical Sciences, Wayne State University School of Medicine, Detroit MI.

Purpose: Inflammation is a key component of retinal disease. We previously reported that exchange protein for cAMP 1 (Epac1) reduced inflammatory mediators, including total levels of high mobility group box 1 (HMGB1) in retinal endothelial cells (RECs) and the mouse retina. The goal of this study was to determine intermediate pathways that allow Epac1 to reduce HMGB1, which could lead to novel targets for therapeutics. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6279194PMC
November 2018
14 Reads

TGF-β-induced IOP elevations are mediated by RhoA in the early but not the late fibrotic phase of open angle glaucoma.

Mol Vis 2018 29;24:712-726. Epub 2018 Oct 29.

Neuroscience and Ophthalmology Research Group, Institute of Inflammation and Ageing, University of Birmingham, Birmingham, United Kingdom.

Purpose: Elevations in intraocular pressure (IOP) are associated with the development of glaucoma and loss of sight. High transforming growth factor-β (TGF-β) 1 levels in the eye's anterior chamber can lead to dysfunctional contractions through RhoA signaling in trabecular meshwork (TM) cells and IOP spikes. Sustained high TGF-β levels leads to TM fibrosis and sustained increases in IOP. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6205807PMC
January 2019
8 Reads

Comprehensive screening of in a cohort of Chinese patients with Bietti crystalline dystrophy.

Mol Vis 2018 26;24:700-711. Epub 2018 Oct 26.

Beijing Institute of Ophthalmology, Beijing Tongren Eye Center, Beijing Tongren Hospital, Capital Medical University; Beijing Ophthalmology & Visual Sciences Key Laboratory, Beijing, China.

Purpose: Bietti crystalline dystrophy (BCD) is an autosomal recessive retinal degeneration disorder caused by mutations in . The aim of this study is to describe the genetic and clinical findings in 128 unrelated Chinese patients diagnosed with BCD.

Methods: Ophthalmological evaluations were performed in all patients. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6204257PMC
January 2019
8 Reads
1.990 Impact Factor

Advanced molecular approaches pave the road to a clear-cut diagnosis of hereditary retinal dystrophies.

Mol Vis 2018 19;24:679-689. Epub 2018 Oct 19.

Genomic Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.

Purpose: The aim of this study was to identify the molecular genetic basis of hereditary retinal dystrophies (HRDs) in five unrelated Iranian families.

Methods: Whole exome sequencing and Sanger sequencing were performed in all families. Variants were analyzed using various bioinformatics databases and software. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6197863PMC
January 2019
2 Reads
1.986 Impact Factor

Non-homologous recombination between Alu and LINE-1 repeats results in a 91 kb deletion in causing severe retinitis pigmentosa.

Mol Vis 2018 18;24:667-678. Epub 2018 Oct 18.

Medical Biosciences/Medical and Clinical Genetics, University of Umeå, Umeå, Sweden.

Purpose: Retinitis pigmentosa (RP) represents a large group of inherited retinal diseases characterized by clinical and genetic heterogeneity. Among patients with RP in northern Sweden, we identified two severely affected siblings and aimed to reveal a genetic cause underlying their disease.

Methods: Whole exome sequencing (WES) was performed on both affected individuals. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6197864PMC
January 2019
1 Read

Set screw homogenization of murine ocular tissue, including the whole eye.

Mol Vis 2018 26;24:690-699. Epub 2018 Oct 26.

Department of Ophthalmology, Emory University, Atlanta, GA.

Purpose: To compare methods for homogenizing the mouse whole eye or retina for RNA extraction.

Methods: We tested five homogenization techniques for the whole eye and the retina. Two established shearing techniques were a version of the Potter-Elvehjem homogenizer, which uses a plastic pellet pestle in a microfuge tube, and a Dounce homogenizer. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6202007PMC
January 2019
10 Reads

Activator protein-1 contributes to the NaCl-induced expression of VEGF and PlGF in RPE cells.

Mol Vis 2018 9;24:647-666. Epub 2018 Oct 9.

Department of Ophthalmology and Eye Hospital, University of Leipzig, Leipzig, Germany.

Purpose: Systemic hypertension is a risk factor of neovascular age-related macular degeneration; consumption of dietary salt resulting in extracellular hyperosmolarity is a main cause of hypertension. Extracellular hyperosmolarity was shown to induce expression of angiogenic growth factors, such as vascular endothelial growth factor (VEGF) and placental growth factor (PlGF), in RPE cells. The aim of the present study was to determine whether the hyperosmotic expression of growth factor genes in RPE cells is mediated by activator protein-1 (AP-1), and whether c-Fos and c-Jun genes are regulated by extracellular osmolarity. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6178337PMC
November 2018
9 Reads

Effects of aging and environmental tobacco smoke exposure on ocular and plasma circulatory microRNAs in the Rhesus macaque.

Mol Vis 2018 24;24:633-646. Epub 2018 Sep 24.

Department of Ophthalmology & Vision Science, School of Medicine, University of California, Davis, Davis, CA.

Purpose: To identify changes induced by environmental tobacco smoke (ETS) in circulatory microRNA (miRNA) in plasma and ocular fluids of the Rhesus macaque and compare these changes to normal age-related changes. Tobacco smoke has been identified as the leading environmental risk factor for age-related macular degeneration (AMD).

Methods: All Rhesus macaques were housed at the California National Primate Research Center (CNPRC), University of California, Davis. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6161805PMC
November 2018
2 Reads

Bilberry extract and anthocyanins suppress unfolded protein response induced by exposure to blue LED light of cells in photoreceptor cell line.

Mol Vis 2018 24;24:621-632. Epub 2018 Sep 24.

Molecular Pharmacology, Department of Biofunctional Evaluation, Gifu Pharmaceutical University, Gifu, Japan.

Purpose: The purpose of this study was to investigate the effects of bilberry extract with its anthocyanins on retinal photoreceptor cell damage and on the endoplasmic reticulum (ER) stress induced by exposure to blue light-emitting diode (LED) light.

Methods: Cultured murine photoreceptor cells (661W) were exposed to blue LED light with or without bilberry extract or its anthocyanins in the culture media. Aggregated short-wavelength opsin (S-opsin) in murine photoreceptor cells was observed with immunostaining. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6161804PMC
November 2018
13 Reads

Elevation of preoperative recipient aqueous cytokine levels in eyes with primary graft failure after corneal transplantation.

Mol Vis 2018 13;24:613-620. Epub 2018 Sep 13.

Department of Ophthalmology, Tokyo Dental College, Ichikawa General Hospital, Chiba, Japan.

Purpose: Primary graft failure after corneal transplantation is caused by dysfunction of corneal endothelial cells. Recently, we demonstrated that preoperative recipients' aqueous cytokine levels are associated with rapid corneal endothelial cell loss after corneal transplantation. In the present study, we evaluated the preoperative inflammatory cytokine levels in the aqueous humor (AqH) of eyes with primary graft failure following corneal transplantation. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6139874PMC
November 2018
3 Reads

Novel homozygous splicing mutations in cause autosomal recessive retinitis pigmentosa.

Mol Vis 2018 31;24:603-612. Epub 2018 Aug 31.

UCL Institute of Ophthalmology, London, UK.

Purpose: Mutations in encoding ADP-ribosylation factor-like 2 binding protein, have recently been implicated as a cause of autosomal recessive retinitis pigmentosa (arRP), with three homozygous variants identified to date. In this study, we performed next-generation sequencing to reveal additional arRP cases associated with variants.

Methods: Whole-genome sequencing (WGS) or whole-exome sequencing (WES) was performed in 1,051 unrelated individuals recruited for the UK Inherited Retinal Disease Consortium and NIHR-BioResource Rare Diseases research studies. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6128700PMC
November 2018
9 Reads
1.990 Impact Factor

Targeted disruption of the endogenous zebrafish locus as models of rapid rod photoreceptor degeneration.

Mol Vis 2018 27;24:587-602. Epub 2018 Aug 27.

Department of Biological Science, Florida State University, Tallahassee, FL.

Purpose: Retinitis pigmentosa (RP) is a collection of genetic disorders that results in the degeneration of light-sensitive photoreceptor cells, leading to blindness. RP is associated with more than 70 loci that may display dominant or recessive modes of inheritance, but mutations in the gene encoding the visual pigment rhodopsin (RHO) are the most frequent cause. In an effort to develop precise mutations in zebrafish as novel models of photoreceptor degeneration, we describe the generation and germline transmission of a series of novel clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9-induced insertion and deletion (indel) mutations in the major zebrafish locus, . Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6128699PMC
November 2018
4 Reads

Phenotypic characterization of patients with early-onset high myopia due to mutations in or : Why not Stickler syndrome?

Mol Vis 2018 10;24:560-573. Epub 2018 Aug 10.

State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-Sen University, Guangzhou, China.

Purpose: Our previous study reported that 5.5% of probands with early-onset high myopia (eoHM) had mutations in or . Why were the probands initially considered to have eoHM but not Stickler syndrome (STL)?

Methods: Probands and family members with eoHM and mutations in or were followed up and reexamined based on the criteria for STL. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6089037PMC
November 2018
1 Read
1.990 Impact Factor

The increased expression of GABA receptors within the arcuate nucleus is associated with high intraocular pressure.

Mol Vis 2018 15;24:574-586. Epub 2018 Aug 15.

Department of Ophthalmology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.

Purpose: To investigate the relationship between intraocular pressure (IOP) and GABA receptors within the arcuate nucleus (ARC).

Methods: In the chronic high IOP rat model, ibotenic acid (IBO) was injected to induce impairment of the ARC, and IOP was measured at the 0, 1, 2, 3, and 4 week time points with a Tono-Pen. To assess the expression of GABA-A/B receptors within the ARC under persistent high IOP, we performed immunofluorescence (IF) and immunohistochemical (IHC) staining at 2 weeks and 4 weeks. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6107798PMC
November 2018
11 Reads

Variants in the gene in a Brazilian population with Stargardt disease.

Mol Vis 2018 1;24:546-559. Epub 2018 Aug 1.

Department of Ophthalmology and Visual Sciences, Federal University of São Paulo (UNIFESP), São Paulo, Brazil.

Purpose: The aim of this study was to analyze and report pathogenic variants in the gene in Brazilian patients with a clinical diagnosis of Stargardt disease.

Methods: This retrospective study evaluated variants in the gene in Brazilian patients with Stargardt disease. The patients' visual acuity and age of symptom onset were obtained from previous medical records. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6070459PMC
November 2018
3 Reads

Microglia increases the proliferation of retinal precursor cells during postnatal development.

Mol Vis 2018 30;24:536-545. Epub 2018 Jul 30.

Molecular Pharmacology, Department of Biofunctional Evaluation, Gifu Pharmaceutical University, Gifu, Japan.

Purpose: In mice, retinal development continues throughout the postnatal stage accompanied by the proliferation of retinal precursor cells. Previous reports showed that during the postnatal stage microglia increase from postnatal day 0 (P0) to P7. However, how microglia are associated with retinal development remains unknown. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6066272PMC
November 2018
16 Reads

Osmotic and hypoxic induction of the complement factor C9 in cultured human retinal pigment epithelial cells: Regulation of VEGF and NLRP3 expression.

Mol Vis 2018 28;24:518-535. Epub 2018 Jul 28.

Department of Ophthalmology and Eye Hospital, University of Leipzig, Leipzig, Germany.

Purpose: Variants of complement factor genes, hypoxia and oxidative stress of the outer retina, and systemic hypertension affect the risk of age-related macular degeneration. Hypertension often results from the high intake of dietary salt that increases extracellular osmolarity. We determined the effects of extracellular hyperosmolarity, hypoxia, and oxidative stress on the expression of complement genes in cultured (dedifferentiated) human RPE cells and investigated the effects of C9 siRNA and C9 protein on RPE cells. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6066273PMC
November 2018
1 Read

Chitosan inhibits inflammation and adipogenesis of orbital fibroblasts in Graves ophthalmopathy.

Mol Vis 2018 26;24:509-517. Epub 2018 Jul 26.

Department of Ophthalmology, the Second Affiliated Hospital of Chongqing Medical University, Chongqing, China.

Purpose: The aim of this study was to investigate the roles of chitosan in inflammation and adipogenesis of primary cultured orbital fibroblasts in Graves ophthalmopathy (GO).

Methods: Cell viability, apoptosis, and cell cycle were determined with the Cell Counting Kit-8 (CCK-8), the Annexin V-FITC/PI kit, and flow cytometry, respectively. Inflammation of orbital fibroblasts was stimulated by interleukin-1 beta (IL-1β). Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6066269PMC
November 2018
6 Reads
1.990 Impact Factor

Protective effect of magnesium acetyltaurate and taurine against NMDA-induced retinal damage involves reduced nitrosative stress.

Mol Vis 2018 25;24:495-508. Epub 2018 Jul 25.

Center for Neuroscience Research, Faculty of Medicine, Universiti Teknologi MARA Sungai Buloh Campus, Selangor, Malaysia.

Purpose: Retinal nitrosative stress associated with altered expression of nitric oxide synthases (NOS) plays an important role in excitotoxic retinal ganglion cell loss in glaucoma. The present study evaluated the effects of magnesium acetyltaurate (MgAT) on changes induced by N-methyl-D-aspartate (NMDA) in the retinal expression of three NOS isoforms, retinal 3-nitrotyrosine (3-NT) levels, and the extent of retinal cell apoptosis in rats. Effects of MgAT with taurine (TAU) alone were compared to understand the benefits of a combined salt of Mg and TAU. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6066271PMC
November 2018
2 Reads
1.990 Impact Factor

Maternal uniparental isodisomy of chromosome 6 unmasks a novel variant in in a patient with early onset retinal dystrophy.

Mol Vis 2018 21;24:478-484. Epub 2018 Jul 21.

Department of Ophthalmology, Flinders University, Flinders Medical Centre, Adelaide, Australia.

Purpose: Inherited retinal dystrophies are a clinically and genetically heterogeneous group of disorders. Molecular diagnosis has proven utility for affected individuals. In this study, we report an individual enrolled in the Australian Inherited Retinal Disease Registry and DNA Bank diagnosed with clinical features overlapping between Leber congenital amaurosis and retinitis pigmentosa. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6066270PMC
November 2018
2 Reads

Mutation profiles of congenital cataract genes in 21 northern Chinese families.

Mol Vis 2018 20;24:471-477. Epub 2018 Jul 20.

Beijing Institute of Ophthalmology, Beijing Tongren Eye Center, Beijing Tongren Hospital of Capital Medical University; Beijing Key Laboratory of Ophthalmology & Visual Sciences, Beijing, China.

Purpose: To identify disease-causing gene mutations in 21 northern Chinese families with congenital cataracts.

Methods: Medical record collection and ophthalmologic examinations were conducted for 21 families with congenital cataracts. A volume of 5 ml of peripheral blood was drawn from each participant for genomic DNA isolation. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6054834PMC
November 2018
23 Reads
1.990 Impact Factor

Anti-inflammatory activity of CKC-containing cationic emulsion eye drop vehicles.

Mol Vis 2018 20;24:459-470. Epub 2018 Jul 20.

SANTEN SAS, Novagali Innovation center, 1, rue Pierre Fontaine, Bâtiment Genavenir IV, F-91058 Evry Cedex, France.

Purpose: Preservative-free cationic emulsion-based artificial tears (ATs) or drug vehicles are innovative eye drop formulations with tear film stabilization and drug delivery properties, and valuable in vivo anti-inflammatory and wound healing properties. These ATs have recently reached the market as ATs for the management of dry eye disease (DED) symptoms (i.e. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6054836PMC
November 2018
7 Reads

Expression patterns of and gene paralogs in developing zebrafish retina.

Mol Vis 2018 19;24:443-458. Epub 2018 Jul 19.

University of Idaho, Department of Biological Sciences, Moscow, ID.

Purpose: The differential adhesion hypothesis states that a cell adhesion code provides cues that direct the specificity of nervous system development. The Down syndrome cell adhesion molecule (DSCAM) and sidekick (SDK) proteins belong to the immunoglobulin superfamily of cell adhesion molecules (CAMs) and provide both attractive and repulsive cues that help to organize the nervous system during development, according to the differential adhesion hypothesis. The zebrafish genome is enriched in and genes, making the zebrafish an excellent model system to further test this hypothesis. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6054835PMC
November 2018
2 Reads

Coexpression of nonvisual opsin, retinal G protein-coupled receptor, and visual pigments in human and bovine cone photoreceptors.

Mol Vis 2018 2;24:434-442. Epub 2018 Jul 2.

Department of Ophthalmology, Keck School of Medicine of the University of Southern California, Los Angeles, CA.

Purpose: Retinal G protein-coupled receptor (RGR) mRNA is transcribed in the outer nuclear layer of human retinas; however, it is not known whether the gene is expressed in the rod or cone photoreceptors. In this study, we investigate broader expression of the normal RGR isoform in photoreceptors of human and bovine retinas.

Methods: We produced and validated a rabbit polyclonal antipeptide antibody (DE15) that is directed against a peptide sequence (SSLLRRWPHGSEGC) partly conserved in RGR across several species. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6031102PMC
November 2018
1 Read

Method for measuring extracellular flux from intact polarized epithelial monolayers.

Mol Vis 2018 25;24:425-433. Epub 2018 Jun 25.

Department of Genetics, Stanford University School of Medicine, Stanford, CA.

Purpose: The Seahorse XFp platform is widely used for metabolic assessment of cultured cells. Current methods require replating of cells into specialized plates. This is problematic for certain cell types, such as primary human fetal RPE (hfRPE) cells, which must be cultured for months to become properly differentiated. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6031101PMC
November 2018
14 Reads

Preoperative profile of inflammatory factors in aqueous humor correlates with postoperative inflammatory response in patients with congenital cataract.

Mol Vis 2018 9;24:414-424. Epub 2018 Jun 9.

State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou, People's Republic of China.

Purpose: To measure the aqueous humor concentrations of inflammatory factors in patients with congenital cataract and to investigate the relationship between the levels and postoperative inflammatory responses.

Methods: Aqueous humor samples were prospectively collected from 65 eyes of children with congenital cataracts from January to June 2015. The levels of 41 inflammation-related cytokines, chemokines, and growth factors in aqueous humor were measured using multiplex bead immunoassay. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5993531PMC
November 2018
3 Reads

Familial congenital cataract, coloboma, and nystagmus phenotype with variable expression caused by mutation in in a South African family.

Mol Vis 2018 9;24:407-413. Epub 2018 Jun 9.

Division of Ophthalmology, Department of Neurosciences, University of Witwatersrand, Johannesburg, South Africa.

Purpose: To report on a clinical and genetic investigation of a large, multigenerational South African family of mixed ancestry with autosomal dominant congenital cataracts, coloboma, and nystagmus.

Methods: Ophthalmic examination was performed in 27 individuals from the same admixed South African family. DNA was sampled from either peripheral blood or buccal swabs in all 27 individuals, and whole genome sequencing was performed in six individuals. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5993530PMC
November 2018
23 Reads

Upregulation of multiple signaling pathways by Dock5 deletion in epithelial cells.

Mol Vis 2017 31;23:1081-1092. Epub 2017 Dec 31.

Department of Oncologic Pathology, Kanazawa Medical University, Ishikawa, Japan.

Purpose: Rupture of lens cataract (RLC) is a hereditary mouse model that shows spontaneous rupture of the lens at the posterior pole at 45-100 days of age. The responsible gene for this phenotype was identified as , a guanine nucleotide exchange factor for small GTPase Rac1. This study was performed to elucidate the pathway initiating this phenotype. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5987311PMC
June 2018
20 Reads

Matrix metalloproteinase-14 is a biomarker of angiogenic activity in proliferative diabetic retinopathy.

Mol Vis 2018 18;24:394-406. Epub 2018 May 18.

Rega Institute for Medical Research, Department of Microbiology and Immunology, University of Leuven, KU Leuven, Leuven, Belgium.

Purpose: Matrix metalloproteinase-14 (MMP-14) is a transmembrane MMP that plays a critical role in promoting angiogenesis. We investigated the expression levels of MMP-14 and correlated the levels with clinical disease activity and with the levels of the angiogenic factors vascular endothelial growth factor (VEGF) and MMP-9 in proliferative diabetic retinopathy (PDR). To reinforce the findings at the functional level, we examined the expression of MMP-14 in the retinas of diabetic rats. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5957543PMC
November 2018
10 Reads
1.990 Impact Factor

Inflammation and outer blood-retina barrier (BRB) compromise following choroidal murine cytomegalovirus (MCMV) infections.

Mol Vis 2018 18;24:379-394. Epub 2018 May 18.

Department of Cellular Biology and Anatomy, Medical College of Georgia, Augusta University, Augusta, GA.

Purpose: The purpose of this study was to determine whether the blood-retina barrier is compromised by choroidal murine cytomegalovirus (MCMV) infection, using electron microscopy.

Methods: BALB/c mice were immunosuppressed with methylprednisolone and monoclonal antibodies to CD4 and CD8. At several time points post-MCMV intraperitoneal inoculation, the eyes were removed and analyzed with western blotting and immunoelectron microscopy for the presence of MCMV early antigen (EA) and the host protein RIP3. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5957546PMC
November 2018
2 Reads

Low-dose minocycline mediated neuroprotection on retinal ischemia-reperfusion injury of mice.

Mol Vis 2018 18;24:367-378. Epub 2018 May 18.

Department of Ophthalmology, The First Affiliated Hospital of Jinan University, Guangzhou, China.

Purpose: The aim of this study was to investigate the effect of minocycline (MC) on the survival of retinal ganglion cells (RGCs) in an ischemic-reperfusion (I/R) injury model of retinal degeneration.

Methods: Retinal I/R injury was induced in the left eye of mice for 60 min by maintaining intraocular pressure at 90 mmHg. Low- or high-dose MC (20 or 100 mg/kg, respectively) was administered by intravenous injection at 5 min after the retinal ischemic insult and then administered once daily until the mice were euthanized. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5957545PMC
November 2018
1 Read

Subtle thinning of retinal layers without overt vascular and inflammatory alterations in a rat model of prediabetes.

Mol Vis 2018 18;24:353-366. Epub 2018 May 18.

Coimbra Institute for Clinical and Biomedical Research (iCBR), Faculty of Medicine, University of Coimbra, Coimbra, Portugal.

Purpose: Diabetic retinopathy is a neurovascular disease characterized by increased permeability of the blood-retinal barrier, changes in the neural components of the retina, and low-grade chronic inflammation. Diabetic retinopathy is a major complication of diabetes; however, the impact of a prediabetic state on the retina remains to be elucidated. The aim of this study was to assess possible early retinal changes in prediabetic rats, by evaluating changes in the integrity of the blood-retinal barrier, the retinal structure, neural markers, and inflammatory mediators. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5957544PMC
November 2018
2 Reads

Placental growth factor promotes epithelial-mesenchymal transition-like changes in ARPE-19 cells under hypoxia.

Mol Vis 2018 26;24:340-352. Epub 2018 Apr 26.

Department of Ophthalmology, the Second Affiliated Hospital of Xi'an Jiaotong University.

Purpose: To investigate the role of placental growth factor (PGF) in the epithelial-mesenchymal transition (EMT) of ARPE-19 cells under hypoxia, and whether the NF-κB signaling pathway is involved in this process.

Methods: ARPE-19 cells were treated in five groups: a control group, hypoxia group, PGF group, hypoxia+PGF group, and NF-κB-blocked group. A chemical hypoxia model was established in the ARPE-19 cells by adding CoCl to the culture medium. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5937674PMC
November 2018
4 Reads

Novel variants identified with next-generation sequencing in Polish patients with cone-rod dystrophy.

Mol Vis 2018 26;24:326-339. Epub 2018 Apr 26.

Department of Medical Genetics, Poznan University of Medical Sciences, Poznan, Poland.

Purpose: The aim of this study was to identify the molecular genetic basis of cone-rod dystrophy in 18 unrelated families of Polish origin. Cone-rod dystrophy is one of the inherited retinal dystrophies, which constitute a highly heterogeneous group of disorders characterized by progressive dysfunction of photoreceptors and retinal pigment epithelium (RPE) cells.

Methods: The study group was composed of four groups of patients representing different Mendelian inheritance of the disease: autosomal dominant (AD), autosomal recessive (AR), X-linked recessive (XL), and autosomal recessive or X-linked recessive (AR/XL). Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5937672PMC
November 2018
10 Reads

Protective effects of Hand.- Mazz. (EBHM) extract in retinal neurodegeneration models.

Mol Vis 2018 25;24:315-325. Epub 2018 Apr 25.

Department of Ophthalmology, Peking University People's Hospital, Beijing, China.

Purpose: To investigate the neuroprotective effects of scutellarin, an active component of the multifunctional traditional Chinese herb (vant.) Hand.-Mazz. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5937673PMC
November 2018
6 Reads

αA-crystallin-derived minichaperone stabilizes αAG98R-crystallin by affecting its zeta potential.

Mol Vis 2018 11;24:297-304. Epub 2018 Apr 11.

Department of Ophthalmology, University of Missouri-Columbia School of Medicine, Columbia, MO.

Purpose: The G98R mutant of αA-crystallin is associated with the development of presenile cataracts. In vitro, the recombinant mutant protein exhibits altered structural and functional characteristics, along with the propensity to aggregate by itself and precipitate. Previously, we have reported that the N-terminal aspartate substituted form of the antiaggregation peptide, DFVIFLDVKHFSPEDLTVK (αA-minichaperone or mini-αA) prevented aggregation of αAG98R. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5906106PMC
November 2018
9 Reads

Structure and microanalysis of tear film ferning of camel tears, human tears, and Refresh Plus.

Mol Vis 2018 16;24:305-314. Epub 2018 Apr 16.

Cornea Research Chair, Department of Optometry, College of Applied Medical Science, King Saud University, Riyadh, Saudi Arabia.

Purpose: This study aimed to investigate the tear ferning pattern and chemical elements of the tear film of camel tears compared with human tears and Refresh Plus eye lubricant. Refresh Plus was used as a control because it provides a healthy ferning pattern, due to the presence of an optimum ratio of carboxymethylcellulose (CMC) sodium and electrolytes. The main research focus is elucidating the viability of camel tear film in the dry, harsh environment of the desert. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5900468PMC
November 2018
3 Reads