7,942 results match your criteria Molecular Immunology [Journal]


Effects of extracellular Hsp70 and cigarette smoke on differentiated THP-1 cells and human monocyte-derived macrophages.

Mol Immunol 2019 Apr 10;111:53-63. Epub 2019 Apr 10.

University of Zagreb, Faculty of Pharmacy and Biochemistry, Department of Medical Biochemistry and Hematology, Zagreb, Croatia. Electronic address:

Extracellular Hsp70 (eHsp70) can act as pro-inflammatory mediator and is elevated in blood of chronic obstructive pulmonary disease (COPD) patients. Most of those patients are smokers, and it was suggested previously that cigarette smoke might induce Hsp70 secretion from the circulating cells. Therefore, we aimed to explore inflammation-associated effects of cigarette smoke extract (CSE) and its combinations with eHsp70 in monocyte-derived macrophages (MDMs) and THP-1 cell line, used as systemic component models of COPD. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S01615890183104
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http://dx.doi.org/10.1016/j.molimm.2019.04.002DOI Listing
April 2019
3 Reads

Structural variants of Salmonella Typhimurium lipopolysaccharide induce less dimerization of TLR4/MD-2 and reduced pro-inflammatory cytokine production in human monocytes.

Mol Immunol 2019 Apr 5;111:43-52. Epub 2019 Apr 5.

Facultad de Química, Universidad Nacional Autónoma de México, Ciudad de México, Mexico. Electronic address:

Salmonella enterica serovar Typhimurium (S. Typhimurium) changes the structure of its lipopolysaccharide (LPS) in response to the environment. The two main LPS variants found in S. Read More

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http://dx.doi.org/10.1016/j.molimm.2019.03.003DOI Listing

PI3K activity in dendritic cells exerts paradoxical effects during autoimmune inflammation.

Mol Immunol 2019 Apr 5;111:32-42. Epub 2019 Apr 5.

Institute for Vascular Biology and Thrombosis Research, Center for Physiology and Pharmacology, Medical University of Vienna, Vienna, Austria; Christian Doppler Laboratory for Arginine Metabolism for Rheumatoid Arthritis and Thrombosis Research, Vienna, Austria. Electronic address:

The peripheral activation of autoreactive T cells and subsequent central nervous system (CNS) immune cell infiltration are key events relevant for experimental autoimmune encephalomyelitis (EAE), a commonly employed multiple sclerosis (MS) model, influenced by T1 and T17 mediated immunity. The phosphoinositide-3-kinase (PI3K)-AKT kinase pathway modulates outcome during EAE, with direct actions of PI3K on adaptive immunity implicated in deleterious and effects on antigen presenting cells involved in beneficial responses during EAE. Here, by genetically deleting the regulatory subunit of Class Ia PI3K, p85α, in selective myeloid cells, we aimed to resolve the impact of PI3K in EAE. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S01615890193003
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http://dx.doi.org/10.1016/j.molimm.2019.03.015DOI Listing
April 2019
3 Reads

IFN-β secretion is through TLR3 but not TLR4 in human gingival epithelial cells.

Mol Immunol 2019 Apr 3;111:27-31. Epub 2019 Apr 3.

Department of Periodontics, School of Dental Medicine, University of Pennsylvania, Philadelphia PA 19004, USA. Electronic address:

The oral cavity is home for a plethora of bacteria and viruses. Epithelial barriers encounter these micro-organisms and recognize them via pathogen recognition receptors (PRRs) that instigate antibacterial and antiviral responses. We and others have shown that human gingival epithelial cells (HGECs) express PRRs to defend invading pathogens. Read More

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http://dx.doi.org/10.1016/j.molimm.2019.03.006DOI Listing

Signaling pathway underlying splenocytes activation by polysaccharides from Atractylodis macrocephalae Koidz.

Mol Immunol 2019 Apr 2;111:19-26. Epub 2019 Apr 2.

Department of Veterinary Medicine, College of Animal Sciences, Zhejiang University, Hangzhou 310058, PR China. Electronic address:

Previous study demonstrated that total polysaccharides isolated from Atractylodis macrocephalae Koidz. (RAMPtp) were effective to eliminate intramammary infection in cows. The present study was designed to investigate the immunomodulatory activity of RAMPtp in mouse splenocytes. Read More

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http://dx.doi.org/10.1016/j.molimm.2019.03.004DOI Listing

The effect of DNA methylation on the miRNA expression pattern in lipopolysaccharide-induced inflammatory responses in human dental pulp cells.

Mol Immunol 2019 Apr 2;111:11-18. Epub 2019 Apr 2.

Guanghua School of Stomatology & Guangdong Provincial Key Laboratory of Stomatology, Sun Yat-sen University, Guangzhou, 510055, Guangdong, People's Republic of China. Electronic address:

Endodontic infection is a widespread oral problem. DNA methylation is a key epigenetic modification that plays important roles in various inflammatory responses, but its role in dental pulp inflammation is poorly understood. In this study, we assessed the expression of DNA methyltransferases (DNMTs) in human dental pulp cells (hDPCs) during lipopolysaccharide (LPS)-induced inflammation and found that DNMT3B mRNA expression was reduced and DNMT1 mRNA and protein levels decreased significantly. Read More

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http://dx.doi.org/10.1016/j.molimm.2019.03.012DOI Listing

Ventilator-induced lung injury is alleviated by inhibiting NLRP3 inflammasome activation.

Mol Immunol 2019 Apr 2;111:1-10. Epub 2019 Apr 2.

Department of Anesthesiology and Perioperative Medicine, Shandong Provincial Qianfoshan Hospital, Shandong University, No. 16766 Jingshi Road, Jinan, 250014, Shandong Province, China. Electronic address:

Background: Mechanical ventilation (MV) is frequently used but can aggravate or cause lung injury, known as ventilator-induced lung injury (VILI). However, the mechanisms are unclear. The NLR family pyrin domain containing 3 (NLRP3) inflammasome is a vital component of innate immunity and is closely related to VILI. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S01615890193005
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http://dx.doi.org/10.1016/j.molimm.2019.03.011DOI Listing
April 2019
3 Reads

A tetravalent single chain diabody (CD40/HER2) efficiently inhibits tumor proliferation through recruitment of T cells and anti-HER2 functions.

Mol Immunol 2019 May 2;109:149-156. Epub 2019 Apr 2.

Tianjin Medical University General Hospital, No. 154 Anshan Road, Heping District, Tianjin 300052, China. Electronic address:

Our aim was to construct a CD40×HER2 single chain diabody (ScDb) and determine its tumor-specific immune activation and anti-HER2 function. Overlap extension-polymerase chain reaction was applied in the construction of ScDb, and the protein was expressed with the pET28a (+)-Rosetta prokaryotic expression system. Soluble ScDb was purified by a nickel-nitrilotriacetic acid column. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S01615890183105
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http://dx.doi.org/10.1016/j.molimm.2019.03.001DOI Listing
May 2019
2 Reads

RS 504393 inhibits M-MDSCs recruiting in immune microenvironment of bladder cancer after gemcitabine treatment.

Mol Immunol 2019 May 2;109:140-148. Epub 2019 Apr 2.

Department of Urology, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China. Electronic address:

Bladder cancer (BC) is a malignant tumor of urinary epithelium. Gemcitabine is an introduced treatment for BC and also has immunomodulatory function, but the immunoregulation mechanism is not clear. In this study, we found that gemcitabine-treated BC cell recruited more monocyte-myeloid-derived suppressed cells (M-MDSCs), which played a significant role in immune suppression and contributed to cancer progression. Read More

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http://dx.doi.org/10.1016/j.molimm.2019.02.014DOI Listing
May 2019
1 Read

Impact of epitope density on CD8+ T cell development and function.

Mol Immunol 2019 Apr 1. Epub 2019 Apr 1.

Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, United States; Department of Pathology and Laboratory Medicine at the Children's Hospital of Philadelphia Research Institute, Philadelphia, PA, United States.

Effective immune responses against intracellular pathogens and tumors frequently rely upon CD8+ cytotoxic T lymphocytes (CTLs). In turn, CTL detection of foreign material from viruses and bacteria depends on antigen presentation by the MHC class I pathway. The underpinnings of antigen processing and presentation and, subsequent T cell activation and immunological memory development, have been extensively studied, leading to a better understanding of the balance between antigen dose, context, and, the T cell activation threshold. Read More

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http://dx.doi.org/10.1016/j.molimm.2019.03.010DOI Listing
April 2019
1 Read

Over expression of bmo-miR-2819 suppresses BmNPV replication by regulating the BmNPV ie-1 gene in Bombyx mori.

Mol Immunol 2019 May 1;109:134-139. Epub 2019 Apr 1.

Sericultural Research Institute, Jiangsu University of Science and Technology, Zhenjiang, Jiangsu, 212018, China. Electronic address:

Bombyx mori nucleopolyhedrovirus (BmNPV) is a major pathogen that threatens the growth and sustainability of the sericulture industry. Accumulating studies in recent years suggest that insect viruses infection can change the host microRNAs (miRNAs) expression profile and both cellular and viral miRNAs play roles in host-pathogen interactions. Until now, the functional analysis of miRNA encoded by silkworm for host-virus interaction is limited. Read More

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http://dx.doi.org/10.1016/j.molimm.2019.03.013DOI Listing
May 2019
1 Read

E3 ubiquitin ligase tripartite motif 7 positively regulates the TLR4-mediated immune response via its E3 ligase domain in macrophages.

Mol Immunol 2019 May 28;109:126-133. Epub 2019 Mar 28.

Department of Emergency Medicine, Jinling Clinical Medical College of Nanjing Medical University, Nanjing, China; Department of Emergency Medicine, Jinling Hospital, Medical School of Nanjing University, Nanjing, China. Electronic address:

Members of the tripartite motif (TRIM) family as E3 ubiquitin ligases have been regarded as critical regulators of innate immunity and antiviral response. However, the role of TRIM7 is still elusive. Here, we provide evidence for the importance of TRIM7 in regulation of the TLR4-mediated innate response. Read More

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http://dx.doi.org/10.1016/j.molimm.2019.01.015DOI Listing
May 2019
2.973 Impact Factor

TARBP2 inhibits IRF7 activation by suppressing TRAF6-mediated K63-linked ubiquitination of IRF7.

Mol Immunol 2019 May 27;109:116-125. Epub 2019 Mar 27.

Key Laboratory of Functional Small Organic Molecules, Ministry of Education and College of Life Science, Jiangxi Normal University, 99 Ziyang Avenue, Nanchang, Jiangxi, 330022, PR China. Electronic address:

Interferon regulatory factor 7 (IRF7), a crucial regulator of type I interferons (IFNs), plays a crucial role in resistance to viral infection. The abnormal production of type I IFNs is associated with many types of disease, such as cancer and inflammatory disorders. Thus, understanding the post-translational modifications of IRF7 is essential to promoting an appropriate immune response. Read More

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http://dx.doi.org/10.1016/j.molimm.2019.02.019DOI Listing
May 2019
1 Read

Transcriptome sequencing to unravel the molecular mechanisms underlying the cuticle liquefaction of Antheraea pernyi following Antheraea pernyi nucleopolyhedrovirus challenge.

Mol Immunol 2019 May 27;109:108-115. Epub 2019 Mar 27.

College of Bioscience and Biotechnology, Liaoning Engineering & Technology Research Center for Insect Resources, Shenyang Agricultural University, Shenyang, 110866, China. Electronic address:

Baculovirus causes liquefaction of insect cuticle to enhance the dissemination of progeny virions away from the host cadavers for increasing viral transmission rates. Antheraea pernyi nucleopolyhedrovirus (ApNPV) infects A. pernyi larvae with circular pus blotches formed in cuticle in the early stage of liquefaction. Read More

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http://dx.doi.org/10.1016/j.molimm.2019.03.009DOI Listing

Immunoregulatory effect of human β-defensin 1 on neonatal cord blood monocyte-derived dendritic cells and T cells.

Mol Immunol 2019 May 26;109:99-107. Epub 2019 Mar 26.

Department of Rheumatology/Immunology, Shanghai Children's Medical Center, Shanghai Jiao Tong University School of Medicine, Shanghai 200127, China; Division of Immunology, Institute of Pediatric Translational Medicine, Shanghai Children's Medical Center, Shanghai Jiao Tong University School of Medicine, Shanghai 200127, China. Electronic address:

The relationship between breastfeeding and infant health has been well elucidated in past decades. Our previous study has shown that human β-defensin 1 (hBD-1) in human breast milk plays a protective role in reducing the incidence of upper respiratory infection in infants younger than 6 months. In the present study, we aim to reveal the mechanism underlying the protective role of hBD-1 by focusing on its immunoregulatory function in neonates. Read More

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http://dx.doi.org/10.1016/j.molimm.2019.03.007DOI Listing
May 2019
2.973 Impact Factor

The prophenoloxidase system in Drosophila participates in the anti-nematode immune response.

Mol Immunol 2019 May 22;109:88-98. Epub 2019 Mar 22.

Infection and Innate Immunity Lab, Department of Biological Sciences, The George Washington University, Washington DC, USA. Electronic address:

Drosophila melanogaster relies on an evolutionarily conserved innate immune system to protect itself from potentially deadly pathogens. One of the earliest pathways activated after injury or infection is the melanization pathway, which is responsible for synthesizing and depositing melanin at the site of injury, or onto invading microbes. Three genes, PPO1-3, encoding prophenoloxidase (PPO), an inactive precursor of phenoloxidase (PO), are responsible for the production of melanin after their activation via immune challenge. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S01615890183073
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http://dx.doi.org/10.1016/j.molimm.2019.03.008DOI Listing
May 2019
2 Reads

Metabolic regulation of T17 cells.

Mol Immunol 2019 May 20;109:81-87. Epub 2019 Mar 20.

Department of Radiation Oncology, The University of Alabama at Birmingham, Birmingham, AL, 35233, United States; Department of Microbiology, The University of Alabama at Birmingham, Birmingham, AL, 35233, United States; Programs in Immunology, The University of Alabama at Birmingham, Birmingham, AL, 35233, United States; O'Neal Comprehensive Cancer Center, The University of Alabama at Birmingham, Birmingham, AL, 35233, United States. Electronic address:

IL-17-producing T17 cells have been associated with autoimmune diseases such as multiple sclerosis (MS), psoriasis, Crohn's disease, and ulcerative colitis (Han et al., 2015), many of which lack effective therapies. Identifying effective approaches to selectively suppress T17 cell development and function represents a legitimate strategy to cure these autoimmune disorders. Read More

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http://dx.doi.org/10.1016/j.molimm.2019.03.005DOI Listing
May 2019
1 Read

The inhibitor of interleukin-3 receptor protects against sepsis in a rat model of cecal ligation and puncture.

Mol Immunol 2019 May 11;109:71-80. Epub 2019 Mar 11.

Weil Institute of Emergency and Critical Care Research, School of Medicine, Virginia Commonwealth University, Richmond, VA, USA; Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou, China. Electronic address:

Sepsis is defined as a life-threatening organ dysfunction caused by a dysregulated host response to infection. There are multiple cytokines involved in the process of sepsis. As an important upstream cytokine in inflammation, Interleukin-3 (IL-3) plays a crucial role during sepsis, however, its exact role is unclear. Read More

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http://dx.doi.org/10.1016/j.molimm.2019.03.002DOI Listing
May 2019
3 Reads

The Mycobacterium tuberculosis glycoprotein Rv1016c protein inhibits dendritic cell maturation, and impairs Th1 /Th17 responses during mycobacteria infection.

Mol Immunol 2019 May 8;109:58-70. Epub 2019 Mar 8.

Guangdong Second Provincial General Hospital, No. 466 Xingang Road, Guangzhou, 510220, China. Electronic address:

The myobacterial factors and the associated mechanism by which Mycobacterium tuberculosis (Mtb) evades the host immune surveillance system remain widely unexplored. Here, we found that overexpressing Rv1016c, a mannosylated protein of M. tuberculosis in BCG (rBCG-Rv1016c) led to increased virulence of the recombined BCG in the severe-combined immunodeficient (SCID) mice model and to a loss of protective efficacy in a zebrafish-M. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S01615890183059
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http://dx.doi.org/10.1016/j.molimm.2019.02.021DOI Listing
May 2019
5 Reads

Human pluripotent stem cell-derived mesenchymal stem cells prevent chronic allergic airway inflammation via TGF-β1-Smad2/Smad3 signaling pathway in mice.

Mol Immunol 2019 May 7;109:51-57. Epub 2019 Mar 7.

Otorhinolaryngology Hospital, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, 510080, China; Centre for Stem Cell Clinical Research and Application, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, 510080, China. Electronic address:

Background: Asthma is a chronic disease involving inflamed airways, which were previously demonstrated, can be modulated by the mesenchymal stem cells derived from induced pluripotent stem cells (iPSC-MSCs). However, the long-term effects of iPSC-MSCs in inflamed airways are still unidentified. This study investigated the long-term effects and potential mechanisms involved in the immunomodulatory effects of iPSC-MSCs in the chronic mouse asthma model. Read More

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http://dx.doi.org/10.1016/j.molimm.2019.02.017DOI Listing
May 2019
2 Reads

Autophagy in regulatory T cells: A double-edged sword in disease settings.

Mol Immunol 2019 May 7;109:43-50. Epub 2019 Mar 7.

The Center for Biomedical Research, Key Laboratory of Organ Transplantation, Ministry of Education, NHC Key Laboratory of Organ Transplantation, Key Laboratory of Organ Transplantation, Chinese Academy of Medical Sciences, Huazhong University of Science and Technology, 1095 Jiefang Ave., Wuhan, 430030, China. Electronic address:

Autophagy is an evolutionarily conserved catabolic process that directs cytoplasmic proteins, organelles and microbes to lysosomes for degradation. It not only represents an essential cell-intrinsic mechanism to protect against internal and external stresses but also shapes both innate and adaptive immunity. Regulatory T cells (Tregs) are a developmentally and functionally distinct T cell subpopulation engaged in sustaining immunological self-tolerance and homeostasis. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S01615890183085
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http://dx.doi.org/10.1016/j.molimm.2019.02.004DOI Listing
May 2019
10 Reads

Molecular mechanism of action responsible for carrageenan-induced inflammatory response.

Mol Immunol 2019 May 7;109:38-42. Epub 2019 Mar 7.

Center for Veterinary Medicine, Office of Research Division of Applied Veterinary Research, 8401 Muirkirk Road, Laurel, MD 20708, United States.

Carrageenan-induced inflammation has long been used as an in vivo model of local inflammation. We developed an in vitro model of inflammation using primary blood cells to characterize gene induction following carrageenan (λ-CGN) stimulation and identify the signal transduction pathway(s) through which λ-CGN worked, using swine whole blood cultures from Yorkshire barrows. Blood samples were divided into stimulated and unstimulated groups. Read More

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http://dx.doi.org/10.1016/j.molimm.2019.02.020DOI Listing
May 2019
2 Reads

Both knock-down and overexpression of Rap2a small GTPase in macrophages result in impairment of NF-κB activity and inflammatory gene expression.

Mol Immunol 2019 May 6;109:27-37. Epub 2019 Mar 6.

Laboratory of Inflammatory Genes, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais (UFMG), Belo Horizonte, MG, Brazil. Electronic address:

Small Ras GTPases are key molecules that regulate a variety of cellular responses in different cell types. Rap1 plays important functions in the regulation of macrophage biology during inflammation triggered by toll-like receptors (TLRs). However, despite sharing a relatively high degree of similarity with Rap1, no studies concerning Rap2 in macrophages and innate immunity have been reported yet. Read More

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http://dx.doi.org/10.1016/j.molimm.2019.02.015DOI Listing
May 2019
6 Reads

PD-1-expressing B cells suppress CD4 and CD8 T cells via PD-1/PD-L1-dependent pathway.

Mol Immunol 2019 May 6;109:20-26. Epub 2019 Mar 6.

Department of Nuclear Medicine, The Affiliated Hospital of Qingdao University, Qingdao, Shandong, 266003, China. Electronic address:

B cell-mediated regulatory function is instrumental to the maintenance of tolerance, but may also contribute to immune dysfunction during infectious diseases and malignancies. In this study, we investigated a subset of B cells characterized by PD-1 expression. Data showed that these PD-1 B cells were rare in peripheral blood, but were significantly upregulated in differentiated thyroid tumors. Read More

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http://dx.doi.org/10.1016/j.molimm.2019.02.009DOI Listing
May 2019
4 Reads

Nanobody against the E7 oncoprotein of human papillomavirus 16.

Mol Immunol 2019 May 5;109:12-19. Epub 2019 Mar 5.

The State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing, Jiangsu, 210046, China; Changzhou High‑Tech Research Institute of Nanjing University and Jiangsu Target Pharma Laboratories, Inc., Changzhou, Jiangsu, 213164, China.

The persistent infection of high-risk human papillomavirus (HPV) is one of the most common causes of cervical cancer. It is well documented that expression of two oncogenes (E6/E7) plays a key role in tumor progression. HPV16E7 -targeting via nanobody (Nb) therefore could be beneficial for HPV16-associated cancer diagnosis and therapy. Read More

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http://dx.doi.org/10.1016/j.molimm.2019.02.022DOI Listing
May 2019
4 Reads
2.973 Impact Factor

Dust mite-derived Der f 3 activates a pro-inflammatory program in airway epithelial cells via PAR-1 and PAR-2.

Mol Immunol 2019 May 2;109:1-11. Epub 2019 Mar 2.

State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen University, China; Department of Biochemistry, School of Life Sciences, Sun Yat-sen University, China. Electronic address:

Protease activity of allergens has been suggested to be involved in the pathogenesis of allergic diseases. The major allergen Der f 3 from Dermatophagoides farinae harbors serine protease activity, but its immunopathogenesis remains unclear. This study aims to explore the effect of Der f 3 on the airway epithelial barrier and on the molecular pathways by which Der f 3 induces inflammation. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S01615890183082
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http://dx.doi.org/10.1016/j.molimm.2019.02.018DOI Listing
May 2019
7 Reads

The ficolin response to LPS challenge in mice.

Mol Immunol 2019 Apr 26;108:121-127. Epub 2019 Feb 26.

Laboratory of Molecular Medicine, Department of Clinical Immunology, Section 7631, Rigshospitalet, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark. Electronic address:

The ficolins belong to an important family of pattern recognition molecules, which contributes to complement activation via the lectin pathway. How the ficolins respond to inflammatory stimuli remains only partly understood. In the present study, we investigated the ficolin A and ficolin B expression and protein distribution patterns in a mouse model of LPS-induced inflammation. Read More

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http://dx.doi.org/10.1016/j.molimm.2019.02.013DOI Listing
April 2019
3 Reads

The ubiquitin-like modifier FAT10 is required for normal IFN-γ production by activated CD8 T cells.

Mol Immunol 2019 Apr 25;108:111-120. Epub 2019 Feb 25.

Division of Immunology, Department of Biology, University of Konstanz, 78457 Konstanz, Germany; Biotechnology Institute Thurgau at the University of Konstanz, 8280 Kreuzlingen, Switzerland. Electronic address:

FAT10 is the only ubiquitin-like modifier which directly targets its substrate proteins for rapid degradation by the proteasome. While the conjugation and proteasomal targeting of FAT10 are fairly well understood, the biological functions of FAT10 have remained largely elusive. Here we have investigated the role of FAT10 in cytokine responses in mice upon viral infection. Read More

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http://dx.doi.org/10.1016/j.molimm.2019.02.010DOI Listing
April 2019
2 Reads

Modified DCs and MSCs with HPV E7 antigen and small Hsps: Which one is the most potent strategy for eradication of tumors?

Mol Immunol 2019 Apr 23;108:102-110. Epub 2019 Feb 23.

National Cell Bank of Iran, Pasteur Institute of Iran, Tehran, Iran.

Immunotherapy with DCs as antigen-presenting vehicles have already improved patients' outcome against a variety of tumors. Moreover, MSCs were recently used to develop anti-cancer therapeutic or anti-microbial prophylactic vaccines. The current study evaluated immune responses and anti-tumor effects generated by DCs and MSCs derived from mouse bone marrow which were modified with small heat shock proteins 27 and 20 (sHsp27 and sHsp20) and also E7 oncoprotein in tumor mouse model. Read More

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http://dx.doi.org/10.1016/j.molimm.2019.02.016DOI Listing
April 2019
2 Reads

Modulation of antiviral immunity by the ichnovirus HdIV in Spodoptera frugiperda.

Mol Immunol 2019 Apr 20;108:89-101. Epub 2019 Feb 20.

UMR 1333 INRA - Université de Montpellier Diversité, Génomes & Interactions Microorganismes-Insectes (DGIMI), 34095 Montpellier, France. Electronic address:

Polydnaviruses (PDVs) are obligatory symbionts found in thousands of endoparasitoid species and essential for successful parasitism. The two genera of PDVs, ichnovirus (IV) and bracovirus (BV), use different sets of virulence factors to ensure successful parasitization of the host. Previous studies have shown that PDVs target apoptosis, one of the innate antiviral responses in many host organisms. Read More

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http://dx.doi.org/10.1016/j.molimm.2019.02.011DOI Listing
April 2019
2 Reads

Phenylethanoid glycosides of Phlomis younghusbandii Mukerjee ameliorate acute hypobaric hypoxia-induced brain impairment in rats.

Mol Immunol 2019 Apr 20;108:81-88. Epub 2019 Feb 20.

Department of Pharmacy, Shaanxi Provincial Hospital of Tuberculosis Prevention and Treatment, Xi'an 710100, PR China. Electronic address:

High altitude cerebral edema (HACE), whose development process is associated with oxidative stress and inflammatory response, is a life-threatening condition caused by rapid ascent speed to high altitudes. Phenylethanoid glycosides (PhGCs) are primary active constituents isolated from Phlomis younghusbandii Mukerjee that reportedly exhibit potent anti-oxidant and anti-inflammatory activities. The present study aims to investigate the protective effect of phenylethanoid glycosides (PhGCs) from P. Read More

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http://dx.doi.org/10.1016/j.molimm.2019.02.002DOI Listing
April 2019
3 Reads

Regulation of chromatin remodeling through RNA polymerase II stalling in the immune system.

Mol Immunol 2019 Apr 19;108:75-80. Epub 2019 Feb 19.

Key Laboratory of Carcinogenesis and Cancer Invasion, Ministry of Education, Xiangya Hospital, Central South University, 87 Xiangya Road, Changsha, Hunan, 410008, China; Key Laboratory of Carcinogenesis, Ministry of Health, Cancer Research Institute, Central South University, 110 Xiangya Road, Changsha, Hunan, 410078, China; Department of Thoracic Surgery, Second Xiangya Hospital, Central South University, Changsha, China. Electronic address:

RNA polymerase II (Pol II) binds to promoter-proximal regions of inducible target genes that are controlled and not transcribed by several negative elongation factors, which is known as Pol II stalling. The occurrence of stalling is due to particular modification signatures and structural conformations of chromatin that affect Pol II elongation. The existence and physiological importance of Pol II stalling implies that there is a dynamic balance in chromatin regulation prior to endogenous or exogenous stimulation. Read More

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http://dx.doi.org/10.1016/j.molimm.2019.02.012DOI Listing
April 2019
2 Reads

Tumor suppressor p53 inhibits porcine epidemic diarrhea virus infection via interferon-mediated antiviral immunity.

Mol Immunol 2019 Apr 18;108:68-74. Epub 2019 Feb 18.

Division of Swine Infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, 678 Haping Road, Harbin, 150069, China. Electronic address:

p53 is a tumor suppressor gene that can be activated in many contexts, such as DNA damage or stressful conditions. p53 has also been shown to be important for responses to certain viral infections. Porcine epidemic diarrhea virus (PEDV) is a major enteric pathogen of the coronavirus family that causes extensive mortality among piglets. Read More

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http://dx.doi.org/10.1016/j.molimm.2019.02.005DOI Listing
April 2019
2 Reads
2.973 Impact Factor

Urinary tract infection: Pathogenicity, antibiotic resistance and development of effective vaccines against Uropathogenic Escherichia coli.

Mol Immunol 2019 Apr 18;108:56-67. Epub 2019 Feb 18.

Department of Molecular Biology, Pasteur Institute of Iran, Pasteur Ave., Tehran, 13164, Iran. Electronic address:

Urinary tract infections (UTIs) are recognized as one of the most common infectious diseases in the world that can be divided to different types. Uropathogenic Escherichia coli (UPEC) strains are the most prevalent causative agent of UTIs that applied different virulence factors such as fimbriae, capsule, iron scavenger receptors, flagella, toxins, and lipopolysaccharide for their pathogenicity in the urinary tract. Despite the high pathogenicity of UPEC strains, host utilizes different immune systems such as innate and adaptive immunity for eradication of them from the urinary tract. Read More

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http://dx.doi.org/10.1016/j.molimm.2019.02.007DOI Listing
April 2019
11 Reads

Heat shock protein 27 inhibits HMGB1 translocation by regulating CBP acetyltransferase activity and ubiquitination.

Mol Immunol 2019 Apr 16;108:45-55. Epub 2019 Feb 16.

Jiangsu Province Key Laboratory for Molecular and Medical Biotechnology, College of Life Science, Nanjing Normal University, Nanjing, Jiangsu, PR China. Electronic address:

Heat-shock protein 27 (Hsp27) is a member of the small heat shock protein family that has been reported to protect cells against pro-inflammatory stresses. High mobility group box 1 (HMGB1) is a proinflammatory cytokine associated with death from sepsis and other inflammatory diseases. After being acetylated by CREB-binding protein (CBP), the transcriptional adaptor and acetyltransferase, HMGB1 translocates from the nucleus to the cytoplasm. Read More

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http://dx.doi.org/10.1016/j.molimm.2019.02.008DOI Listing
April 2019
2 Reads

Measuring anti-HLA antibody active concentration and affinity by surface plasmon resonance: Comparison with the luminex single antigen flow beads and T-cell flow cytometry crossmatch results.

Mol Immunol 2019 Apr 16;108:34-44. Epub 2019 Feb 16.

CHU de Bordeaux, Laboratoire d'Immunologie et Immunogénétique, Hôpital Pellegrin, Place Amélie Raba Léon, Bordeaux, France; Immuno ConcEpT, UMR CNRS 5164, 146 rue Léo Saignat, Bordeaux, France; Université de Bordeaux, 146 rue Léo Saignat, Bordeaux, France.

Background: Although the Luminex single antigen flow beads (SAFB) and the flow cytometry crossmatch (FCXM) are the most sensitive assays used for anti-HLA antibodies characterization in transplant recipients, their semi-quantitative fluorescence read-out is not closely linked to graft outcome.

Methods: Surface plasmon resonance (SPR) was implemented to determine truly quantitative parameters of five human monoclonal anti-class I HLA antibodies (mAbs): first the active concentration and then the binding constants. The results were compared to those obtained with SAFB and T-cell FCXM (T-FCXM). Read More

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http://dx.doi.org/10.1016/j.molimm.2019.02.006DOI Listing
April 2019
3 Reads

Cytokines use different intracellular mechanisms to upregulate the membrane expression of CXCR1 in human monocytes.

Mol Immunol 2019 Apr 16;108:23-33. Epub 2019 Feb 16.

Laboratorio de Patogénesis e Inmunología de Procesos Infecciosos, Instituto de Medicina Experimental, Consejo Nacional de Investigaciones Científicas y Técnicas- Academia Nacional de Medicina, Buenos Aires, Argentina. Electronic address:

Membrane expression of fractalkine (CXCL1)-receptor (CXCR1) is relevant in monocytes (Mo) because CXCR1-CXCL1 interactions might participate on both, homeostatic and pathologic conditions. We have previously demonstrated that CXCR1 levels are decreased during culture and when Mo are differentiated into dendritic cells, but enhanced when differentiated into macrophages. Regarding soluble factors, lipopolysaccharide (LPS) accelerated the loss of CXCR1, while interleukin (IL)-10 and Interferon-gamma (IFN-γ) prevented it. Read More

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http://dx.doi.org/10.1016/j.molimm.2019.01.003DOI Listing
April 2019
6 Reads

Circulating CD14CD163CD209 M2-like monocytes are associated with the severity of infection in Helicobacter pylori-positive patients.

Mol Immunol 2019 Apr 13;108:13-22. Epub 2019 Feb 13.

Genetic Diagnosis Center, The First Hospital of Jilin University, Changchun 130021, China; Key Laboratory of Zoonosis Research, Ministry of Education, The First Hospital, Jilin University, Changchun 130021, China; Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou 225009, China. Electronic address:

Helicobacter pylori (H. pylori) initiates a robust host immune response and subsequently results in chronic inflammation in the gastric mucosa. This study monitored the circulating monocyte subsets and measured the plasma levels of IL-10 and IL-12 in response to H. Read More

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http://dx.doi.org/10.1016/j.molimm.2019.01.017DOI Listing
April 2019
6 Reads

Inhibition of the alternative complement pathway accelerates repair processes in the murine model of choroidal neovascularization.

Mol Immunol 2019 Apr 12;108:8-12. Epub 2019 Feb 12.

Department of Ophthalmology and Microbiology, Medical University of South Carolina, Charleston, SC 29425, United States; Department of Ralph H. Johnson VA Medical Center, Division of Research, Charleston, SC 29401, United States. Electronic address:

Age-related macular degeneration (AMD) is the leading cause of blindness in the US. Polymorphisms in complement components are associated with increased AMD risk, and it has been hypothesized that an overactive complement system is partially responsible for AMD pathology. Choroidal neovascularization (CNV) has two phases, injury/angiogenesis and repair/fibrosis. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S01615890183096
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http://dx.doi.org/10.1016/j.molimm.2019.02.001DOI Listing
April 2019
9 Reads

Inflammasome activation and Th17 responses.

Mol Immunol 2019 Mar 8;107:142-164. Epub 2019 Feb 8.

Advanced Medical Research Institute, Shandong University, Jinan, Shandong 250012, China; School of Biomedical Sciences, The University of Hong Kong, Pokfulam, Hong Kong. Electronic address:

Immune sensing of exogenous molecules from microbes (e.g., pathogen-associated molecular patterns) and nonmicrobial molecules (e. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S01615890183105
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http://dx.doi.org/10.1016/j.molimm.2018.12.024DOI Listing
March 2019
15 Reads

Fli-1 transcription factor regulates the expression of caspase-1 in lung pericytes.

Mol Immunol 2019 Apr 8;108:1-7. Epub 2019 Feb 8.

Department of Pathology and Laboratory Medicine, Medical University of South Carolina, Charleston, SC, 29425, United States; Department of Regenerative Medicine and Cell Biology, Medical University of South Carolina, Charleston, SC, 29425, United States. Electronic address:

Our previous data demonstrated that Friend leukemia virus integration 1 (Fli-1), an ETS transcription factor, governs pericyte loss and vascular dysfunction in cecal ligation and puncture-induced murine sepsis by regulating essential pyroptosis markers including caspase-1. However, whether Fli-1 regulates caspase-1 expression levels in vitro and how Fli-1 regulates caspase-1 remain unknown. Our present work further demonstrated that overexpressed Fli-1 significantly increased caspase-1 and IL-18 expression levels in cultured mouse lung pericytes. Read More

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http://dx.doi.org/10.1016/j.molimm.2019.02.003DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6409192PMC
April 2019
4 Reads

MLKL deficiency inhibits DSS-induced colitis independent of intestinal microbiota.

Mol Immunol 2019 Mar 7;107:132-141. Epub 2019 Feb 7.

Key Laboratory of Zoonosis Research, Ministry of Education, College of Veterinary Medicine, Jilin University, Changchun, China. Electronic address:

The maintenance of intestinal tissue homeostasis is vital for the resistance against inflammatory bowel diseases (IBDs). Necroptosis is identified as an alternative mode of regulated cell death, which plays a pivotal role in tissue homeostasis. Thus, the roles of RIP3-mediated necroptosis in intestinal inflammation have been extensively studied. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S01615890183104
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http://dx.doi.org/10.1016/j.molimm.2019.01.018DOI Listing
March 2019
21 Reads
2.973 Impact Factor

CD226 is involved in megakaryocyte activation and early-stage differentiation.

Mol Immunol 2019 Mar 6;107:123-131. Epub 2019 Feb 6.

Department of Immunology, the fourth Military Medical University, #1 Changle Xi Road, Xi'an, PR China; Transplant Immunology Laboratory, the Fourth Military Medical University, #1 Changle Xi Road, Xi'an, PR China. Electronic address:

This study was conducted to investigate the effect of CD226 on the differentiation, activation, and polyploidization of megakaryocytes (MKs) and explore the potential mechanism. Dami (megakaryocyte line) cell maturation was induced by phorbol 12-myristate 13-acetate. CD226 was silenced by infection with a CD226-specific shRNA lentiviral vector. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S01615890183080
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http://dx.doi.org/10.1016/j.molimm.2019.01.013DOI Listing
March 2019
13 Reads
2.973 Impact Factor

Corrigendum to "Using single cell analysis for translational studies in immune mediated diseases: Opportunities and challenges" [Mol. Immunol. 103 (2018) 191-199].

Mol Immunol 2019 Mar 4;107:165. Epub 2019 Feb 4.

Department of Immunology and Microbiology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA, 92037, USA. Electronic address:

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http://dx.doi.org/10.1016/j.molimm.2019.01.011DOI Listing
March 2019
2 Reads
2.973 Impact Factor

MdRDH1, a HSP67B2-like rhodanese homologue plays a positive role in maintaining redox balance in Musca domestica.

Mol Immunol 2019 Mar 1;107:115-122. Epub 2019 Feb 1.

The Key Laboratory of Zoological Systematics and Application, College of Life Sciences, Hebei University, Baoding, Hebei, 071002, China. Electronic address:

Rhodanese homology domains (RHODs) are the structural modules of ubiquitous tertiary that occur in three major evolutionary phyla. Despite the versatile and important physiological functions of RHODs containing proteins, little is known about their invertebrate counterparts. A novel HSP67B2-like single-domain rhodanese homologue, MdRDH1 from Musca domestica, whose expression can be induced by bacterial infection or oxidative stress. Read More

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http://dx.doi.org/10.1016/j.molimm.2019.01.016DOI Listing
March 2019
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Fas/FasL signaling is critical for the survival of exhausted antigen-specific CD8 T cells during tumor immune response.

Mol Immunol 2019 Mar 30;107:97-105. Epub 2019 Jan 30.

Department of General Surgical Science, Gunma University Graduate School of Medicine, 3-39-22, Showa, Maebashi 371-8511, Japan.

Antigen (Ag)-specific activated CD8 T cells are critical for tumor elimination but become exhausted, and thus, dysfunctional during immune response against the tumor due to chronic antigen stimulation. The signaling of immune checkpoint receptors is known to be a critical component in this exhaustion; however, the fate of these exhausted CD8 T cells remains unclear. Therefore, to elucidate this, we followed the fate of Ag-specific CD8 T cells by directly visualizing them using MHC class I tetramers coupled with ovoalubumin in C57BL/6 mice inoculated with EG. Read More

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http://dx.doi.org/10.1016/j.molimm.2019.01.014DOI Listing
March 2019
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Identification and characterisation of conserved epitopes of E. ruminantium that activate Th1 CD4 T cells: Towards the development of a multi-epitope vaccine.

Mol Immunol 2019 Mar 31;107:106-114. Epub 2019 Jan 31.

Agricultural Research Council-Onderstepoort Veterinary Research, Private Bag X5, Onderstepoort, 0110, South Africa; Department of Veterinary Tropical Diseases, University of Pretoria, Private Bag X04, Onderstepoort, 0110, South Africa.

Several studies have shown that cytotoxic T lymphocytes (CTL) require CD4 + Th1 epitopes to generate strong immune responses to intracellular pathogens. However, not much is known about Ehrlichia ruminantium epitopes, particularly those that can be considered potential candidates for inclusion in a multi-epitope vaccine. In order to identify CD4 Th1 epitopes that induce IFNγ, a number of proteins previously identified as immunogenic were first screened to determine if they induce cellular immunity in tick infected immune sheep PBMC. Read More

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http://dx.doi.org/10.1016/j.molimm.2018.12.012DOI Listing
March 2019
3 Reads

JAK/STAT proteins and their biological impact on NK cell development and function.

Mol Immunol 2019 Jan 28. Epub 2019 Jan 28.

Department of Pediatrics, Baylor College of Medicine, Houston, TX, USA; Texas Children's Hospital, Center for Human Immunobiology, Department of Allergy, Immunology and Rheumatology, Houston, TX, USA. Electronic address:

NK cells are important early effectors in the innate immune response to a variety of viral infections and for elimination of tumor cells. The JAK/STAT signaling cascade is critical for NK cell development, maturation, survival, and proliferation, therefore, it is important to understand the role of this pathway in NK cell biology. Many cytokines can activate multiple JAK/STAT protein family members, creating a severe phenotype when mutations impair their function or expression. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S01615890183101
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http://dx.doi.org/10.1016/j.molimm.2018.12.005DOI Listing
January 2019
9 Reads

SERPING1 exon 3 splicing variants using alternative acceptor splice sites.

Mol Immunol 2019 Mar 24;107:91-96. Epub 2019 Jan 24.

Centre for Cardiovascular Surgery and Transplantation, Brno, Czech Republic; Department of Clinical Immunology and Allergology, Faculty of Medicine, Masaryk University, Brno, Czech Republic.

Mutations in the C1 inhibitor (C1INH) encoding gene, SERPING1, are associated with hereditary angioedema (HAE) which manifests as recurrent submucosal and subcutaneous edema episodes. The major C1INH function is the complement system inhibition, preventing its spontaneous activation. The presented study is focused on SERPING1 exon 3, an alternative and extraordinarily long exon (499 bp). Read More

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http://dx.doi.org/10.1016/j.molimm.2019.01.007DOI Listing
March 2019
5 Reads

hsa-miR-20a-5p attenuates allergic inflammation in HMC-1 cells by targeting HDAC4.

Mol Immunol 2019 Mar 23;107:84-90. Epub 2019 Jan 23.

Department of Pediatrics, Nanjing Medical University Affiliated Jiangning Hospital, 168 Gushan Road, Nanjing 211100, Jiangsu Province, China. Electronic address:

micro-RNAs (miRNAs) are non-coding RNAs which play important role in human diseases. Dysregulated miRNAs have been identified in asthma patient while their precise roles in asthma are not well elucidated. We compared the expression level of total 11 miRNAs between PMA/A23187-treated and control HMC-1 mast cells. Read More

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http://dx.doi.org/10.1016/j.molimm.2019.01.010DOI Listing
March 2019
3 Reads