7,907 results match your criteria Molecular Immunology [Journal]


Measuring anti-HLA antibody active concentration and affinity by surface plasmon resonance: Comparison with the luminex single antigen flow beads and T-cell flow cytometry crossmatch results.

Mol Immunol 2019 Feb 15;108:34-44. Epub 2019 Feb 15.

CHU de Bordeaux, Laboratoire d'Immunologie et Immunogénétique, Hôpital Pellegrin, Place Amélie Raba Léon, Bordeaux, France; Immuno ConcEpT, UMR CNRS 5164, 146 rue Léo Saignat, Bordeaux, France; Université de Bordeaux, 146 rue Léo Saignat, Bordeaux, France.

Background: Although the Luminex single antigen flow beads (SAFB) and the flow cytometry crossmatch (FCXM) are the most sensitive assays used for anti-HLA antibodies characterization in transplant recipients, their semi-quantitative fluorescence read-out is not closely linked to graft outcome.

Methods: Surface plasmon resonance (SPR) was implemented to determine truly quantitative parameters of five human monoclonal anti-class I HLA antibodies (mAbs): first the active concentration and then the binding constants. The results were compared to those obtained with SAFB and T-cell FCXM (T-FCXM). Read More

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http://dx.doi.org/10.1016/j.molimm.2019.02.006DOI Listing
February 2019

Cytokines use different intracellular mechanisms to upregulate the membrane expression of CXCR1 in human monocytes.

Mol Immunol 2019 Feb 15;108:23-33. Epub 2019 Feb 15.

Laboratorio de Patogénesis e Inmunología de Procesos Infecciosos, Instituto de Medicina Experimental, Consejo Nacional de Investigaciones Científicas y Técnicas- Academia Nacional de Medicina, Buenos Aires, Argentina. Electronic address:

Membrane expression of fractalkine (CXCL1)-receptor (CXCR1) is relevant in monocytes (Mo) because CXCR1-CXCL1 interactions might participate on both, homeostatic and pathologic conditions. We have previously demonstrated that CXCR1 levels are decreased during culture and when Mo are differentiated into dendritic cells, but enhanced when differentiated into macrophages. Regarding soluble factors, lipopolysaccharide (LPS) accelerated the loss of CXCR1, while interleukin (IL)-10 and Interferon-gamma (IFN-γ) prevented it. Read More

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http://dx.doi.org/10.1016/j.molimm.2019.01.003DOI Listing
February 2019

Circulating CD14CD163CD209 M2-like monocytes are associated with the severity of infection in Helicobacter pylori-positive patients.

Mol Immunol 2019 Feb 13;108:13-22. Epub 2019 Feb 13.

Genetic Diagnosis Center, The First Hospital of Jilin University, Changchun 130021, China; Key Laboratory of Zoonosis Research, Ministry of Education, The First Hospital, Jilin University, Changchun 130021, China; Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou 225009, China. Electronic address:

Helicobacter pylori (H. pylori) initiates a robust host immune response and subsequently results in chronic inflammation in the gastric mucosa. This study monitored the circulating monocyte subsets and measured the plasma levels of IL-10 and IL-12 in response to H. Read More

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http://dx.doi.org/10.1016/j.molimm.2019.01.017DOI Listing
February 2019
1 Read

Inhibition of the alternative complement pathway accelerates repair processes in the murine model of choroidal neovascularization.

Mol Immunol 2019 Feb 11;108:8-12. Epub 2019 Feb 11.

Department of Ophthalmology and Microbiology, Medical University of South Carolina, Charleston, SC 29425, United States; Department of Ralph H. Johnson VA Medical Center, Division of Research, Charleston, SC 29401, United States. Electronic address:

Age-related macular degeneration (AMD) is the leading cause of blindness in the US. Polymorphisms in complement components are associated with increased AMD risk, and it has been hypothesized that an overactive complement system is partially responsible for AMD pathology. Choroidal neovascularization (CNV) has two phases, injury/angiogenesis and repair/fibrosis. Read More

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http://dx.doi.org/10.1016/j.molimm.2019.02.001DOI Listing
February 2019
1 Read

Inflammasome activation and Th17 responses.

Mol Immunol 2019 Mar 8;107:142-164. Epub 2019 Feb 8.

Advanced Medical Research Institute, Shandong University, Jinan, Shandong 250012, China; School of Biomedical Sciences, The University of Hong Kong, Pokfulam, Hong Kong. Electronic address:

Immune sensing of exogenous molecules from microbes (e.g., pathogen-associated molecular patterns) and nonmicrobial molecules (e. Read More

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http://dx.doi.org/10.1016/j.molimm.2018.12.024DOI Listing
March 2019
4 Reads

Fli-1 transcription factor regulates the expression of caspase-1 in lung pericytes.

Mol Immunol 2019 Feb 7;108:1-7. Epub 2019 Feb 7.

Department of Pathology and Laboratory Medicine, Medical University of South Carolina, Charleston, SC, 29425, United States; Department of Regenerative Medicine and Cell Biology, Medical University of South Carolina, Charleston, SC, 29425, United States. Electronic address:

Our previous data demonstrated that Friend leukemia virus integration 1 (Fli-1), an ETS transcription factor, governs pericyte loss and vascular dysfunction in cecal ligation and puncture-induced murine sepsis by regulating essential pyroptosis markers including caspase-1. However, whether Fli-1 regulates caspase-1 expression levels in vitro and how Fli-1 regulates caspase-1 remain unknown. Our present work further demonstrated that overexpressed Fli-1 significantly increased caspase-1 and IL-18 expression levels in cultured mouse lung pericytes. Read More

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http://dx.doi.org/10.1016/j.molimm.2019.02.003DOI Listing
February 2019

MLKL deficiency inhibits DSS-induced colitis independent of intestinal microbiota.

Mol Immunol 2019 Mar 7;107:132-141. Epub 2019 Feb 7.

Key Laboratory of Zoonosis Research, Ministry of Education, College of Veterinary Medicine, Jilin University, Changchun, China. Electronic address:

The maintenance of intestinal tissue homeostasis is vital for the resistance against inflammatory bowel diseases (IBDs). Necroptosis is identified as an alternative mode of regulated cell death, which plays a pivotal role in tissue homeostasis. Thus, the roles of RIP3-mediated necroptosis in intestinal inflammation have been extensively studied. Read More

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http://dx.doi.org/10.1016/j.molimm.2019.01.018DOI Listing
March 2019
2 Reads
2.973 Impact Factor

CD226 is involved in megakaryocyte activation and early-stage differentiation.

Mol Immunol 2019 Mar 6;107:123-131. Epub 2019 Feb 6.

Department of Immunology, the fourth Military Medical University, #1 Changle Xi Road, Xi'an, PR China; Transplant Immunology Laboratory, the Fourth Military Medical University, #1 Changle Xi Road, Xi'an, PR China. Electronic address:

This study was conducted to investigate the effect of CD226 on the differentiation, activation, and polyploidization of megakaryocytes (MKs) and explore the potential mechanism. Dami (megakaryocyte line) cell maturation was induced by phorbol 12-myristate 13-acetate. CD226 was silenced by infection with a CD226-specific shRNA lentiviral vector. Read More

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http://dx.doi.org/10.1016/j.molimm.2019.01.013DOI Listing
March 2019
2.973 Impact Factor

Corrigendum to "Using single cell analysis for translational studies in immune mediated diseases: Opportunities and challenges" [Mol. Immunol. 103 (2018) 191-199].

Mol Immunol 2019 Mar 4;107:165. Epub 2019 Feb 4.

Department of Immunology and Microbiology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA, 92037, USA. Electronic address:

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http://dx.doi.org/10.1016/j.molimm.2019.01.011DOI Listing

MdRDH1, a HSP67B2-like rhodanese homologue plays a positive role in maintaining redox balance in Musca domestica.

Mol Immunol 2019 Mar 1;107:115-122. Epub 2019 Feb 1.

The Key Laboratory of Zoological Systematics and Application, College of Life Sciences, Hebei University, Baoding, Hebei, 071002, China. Electronic address:

Rhodanese homology domains (RHODs) are the structural modules of ubiquitous tertiary that occur in three major evolutionary phyla. Despite the versatile and important physiological functions of RHODs containing proteins, little is known about their invertebrate counterparts. A novel HSP67B2-like single-domain rhodanese homologue, MdRDH1 from Musca domestica, whose expression can be induced by bacterial infection or oxidative stress. Read More

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http://dx.doi.org/10.1016/j.molimm.2019.01.016DOI Listing

Fas/FasL signaling is critical for the survival of exhausted antigen-specific CD8 T cells during tumor immune response.

Mol Immunol 2019 Mar 30;107:97-105. Epub 2019 Jan 30.

Department of General Surgical Science, Gunma University Graduate School of Medicine, 3-39-22, Showa, Maebashi 371-8511, Japan.

Antigen (Ag)-specific activated CD8 T cells are critical for tumor elimination but become exhausted, and thus, dysfunctional during immune response against the tumor due to chronic antigen stimulation. The signaling of immune checkpoint receptors is known to be a critical component in this exhaustion; however, the fate of these exhausted CD8 T cells remains unclear. Therefore, to elucidate this, we followed the fate of Ag-specific CD8 T cells by directly visualizing them using MHC class I tetramers coupled with ovoalubumin in C57BL/6 mice inoculated with EG. Read More

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http://dx.doi.org/10.1016/j.molimm.2019.01.014DOI Listing

Identification and characterisation of conserved epitopes of E. ruminantium that activate Th1 CD4 T cells: Towards the development of a multi-epitope vaccine.

Mol Immunol 2019 Mar 31;107:106-114. Epub 2019 Jan 31.

Agricultural Research Council-Onderstepoort Veterinary Research, Private Bag X5, Onderstepoort, 0110, South Africa; Department of Veterinary Tropical Diseases, University of Pretoria, Private Bag X04, Onderstepoort, 0110, South Africa.

Several studies have shown that cytotoxic T lymphocytes (CTL) require CD4 + Th1 epitopes to generate strong immune responses to intracellular pathogens. However, not much is known about Ehrlichia ruminantium epitopes, particularly those that can be considered potential candidates for inclusion in a multi-epitope vaccine. In order to identify CD4 Th1 epitopes that induce IFNγ, a number of proteins previously identified as immunogenic were first screened to determine if they induce cellular immunity in tick infected immune sheep PBMC. Read More

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http://dx.doi.org/10.1016/j.molimm.2018.12.012DOI Listing
March 2019
1 Read

JAK/STAT proteins and their biological impact on NK cell development and function.

Mol Immunol 2019 Jan 28. Epub 2019 Jan 28.

Department of Pediatrics, Baylor College of Medicine, Houston, TX, USA; Texas Children's Hospital, Center for Human Immunobiology, Department of Allergy, Immunology and Rheumatology, Houston, TX, USA. Electronic address:

NK cells are important early effectors in the innate immune response to a variety of viral infections and for elimination of tumor cells. The JAK/STAT signaling cascade is critical for NK cell development, maturation, survival, and proliferation, therefore, it is important to understand the role of this pathway in NK cell biology. Many cytokines can activate multiple JAK/STAT protein family members, creating a severe phenotype when mutations impair their function or expression. Read More

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http://dx.doi.org/10.1016/j.molimm.2018.12.005DOI Listing
January 2019
1 Read

SERPING1 exon 3 splicing variants using alternative acceptor splice sites.

Mol Immunol 2019 Mar 24;107:91-96. Epub 2019 Jan 24.

Centre for Cardiovascular Surgery and Transplantation, Brno, Czech Republic; Department of Clinical Immunology and Allergology, Faculty of Medicine, Masaryk University, Brno, Czech Republic.

Mutations in the C1 inhibitor (C1INH) encoding gene, SERPING1, are associated with hereditary angioedema (HAE) which manifests as recurrent submucosal and subcutaneous edema episodes. The major C1INH function is the complement system inhibition, preventing its spontaneous activation. The presented study is focused on SERPING1 exon 3, an alternative and extraordinarily long exon (499 bp). Read More

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http://dx.doi.org/10.1016/j.molimm.2019.01.007DOI Listing
March 2019
2 Reads

hsa-miR-20a-5p attenuates allergic inflammation in HMC-1 cells by targeting HDAC4.

Mol Immunol 2019 Mar 23;107:84-90. Epub 2019 Jan 23.

Department of Pediatrics, Nanjing Medical University Affiliated Jiangning Hospital, 168 Gushan Road, Nanjing 211100, Jiangsu Province, China. Electronic address:

micro-RNAs (miRNAs) are non-coding RNAs which play important role in human diseases. Dysregulated miRNAs have been identified in asthma patient while their precise roles in asthma are not well elucidated. We compared the expression level of total 11 miRNAs between PMA/A23187-treated and control HMC-1 mast cells. Read More

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http://dx.doi.org/10.1016/j.molimm.2019.01.010DOI Listing
March 2019
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Production, purification, and evaluation of quail immunoglobulin Y against Salmonella typhimurium and Salmonella enteritidis.

Mol Immunol 2019 Mar 18;107:79-83. Epub 2019 Jan 18.

Department of Pathobiology, School of Veterinary Medicine, Shiraz University, Shiraz, Iran.

Salmonella species have been the major foodborne problems in food production systems, with Salmonella enterica serovars typhimurium (S. typhimurium) and enteritidis (S. enteritidis) being among the more common isolates. Read More

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http://dx.doi.org/10.1016/j.molimm.2019.01.012DOI Listing
March 2019
6 Reads

Role of interleukin-17 in acute myocardial infarction.

Mol Immunol 2019 Mar 17;107:71-78. Epub 2019 Jan 17.

Unidad de Investigación Médica en Inmunología, UMAE, Hospital de Pediatría, Centro Médico Nacional Siglo XXI, Instituto Mexicano del Seguro Social, Ciudad de México, México. Electronic address:

Acute myocardial infarction (AMI) is a leading cause of death worldwide. Myocardial necrosis generates damage signals and triggers an intense inflammatory response. Many cytokines that contribute to repair tissue can also cause adverse left ventricular remodeling and heart failure. Read More

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http://dx.doi.org/10.1016/j.molimm.2019.01.008DOI Listing
March 2019
3 Reads

Ebi3 promotes T- and B-cell division and differentiation via STAT3.

Mol Immunol 2019 Mar 17;107:61-70. Epub 2019 Jan 17.

Laboratory of Immunology, Institute of Basic Medical Sciences, Beijing 100850, China. Electronic address:

Although sharing the same subunit Ebi3, IL-27 (p28/Ebi3) and IL-35 (p35/Ebi3) have different biological functions, suggesting that Ebi3 subunit may functions as a carrier. Our data demonstrated that activated T cells and B cells effectively up-regulated Ebi3 expression. In addition, Ebi3 effectively promoted T-cell activation and the differentiation of helper T 1 (Th1), Th17, and Foxp3 regulatory T (Treg) cells induced by Th1, Th17, and Treg polarizing condition, respectively. Read More

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http://dx.doi.org/10.1016/j.molimm.2019.01.009DOI Listing
March 2019
4 Reads

Anagliptin ameliorates high glucose- induced endothelial dysfunction via suppression of NLRP3 inflammasome activation mediated by SIRT1.

Mol Immunol 2019 Mar 17;107:54-60. Epub 2019 Jan 17.

Department of Anesthesiology, China-Japan Union Hospital of Jilin University, Changchun, 130033, China.

High glucose- induced endothelial dysregulation has been recognized as an initiation of vascular complications in Type 2 diabetes mellitus (T2DM). Anagliptin is a novel licensed dipeptidyl peptidase-4 (DPP-4) inhibitor for the treatment of T2DM. The effects of anagliptin in high glucose- induced endothelial dysfunction are less reported. Read More

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http://dx.doi.org/10.1016/j.molimm.2019.01.006DOI Listing

Granulysin species segregate to different lysosome-related effector vesicles (LREV) and get mobilized by either classical or non-classical degranulation.

Mol Immunol 2019 Mar 16;107:44-53. Epub 2019 Jan 16.

Molecular Immunology, Institute of Immunology, Christian-Albrechts-University of Kiel and University Hospital Schleswig-Holstein Campus Kiel, Arnold-Heller-Str. 3, Building 17, D-24105 Kiel, Germany. Electronic address:

Granulysin (GNLY) is a cationic antimicrobial, proinflammatory, and cytotoxic effector protein primarily expressed in human cytotoxic T and NK cells. Its two variants, the 15 kDa precursor and the mature 9 kDa protein processed by proteolysis, act on different microbes or infected and transformed target cells and utilize mechanistically different effector activities. In human peripheral blood lymphocytes of healthy individuals, both forms of GNLY are detected in TCR αβ (CD4 and CD8) T cells, TCR γδ T cells, and CD3CD56 NK cells. Read More

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http://dx.doi.org/10.1016/j.molimm.2018.12.031DOI Listing
March 2019
3 Reads

New Aspect of Liver IL-17γδ T Cells.

Mol Immunol 2019 Mar 12;107:41-43. Epub 2019 Jan 12.

The First Affiliated Hospital, Biomedical Translational Research Institute and School of Pharmacy, Jinan University, Guangzhou, 510632, China; The Sixth Affiliated Hospital of Guangzhou Medical University, Qingyuan People's Hospital, Qingyuan, 511518, China. Electronic address:

Liver is a critical organ where comprehensive immune regulation or defense occurs. γδ T cells in liver represent indispensable population and are found to be regulating a variety of diseases including autoimmunity, cancer, fibrosis, and infections. IL-17 γδ T cells in liver is a functional subset which has been found to have pro-inflammatory effect or anti-infection ability. Read More

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http://dx.doi.org/10.1016/j.molimm.2018.12.030DOI Listing
March 2019
8 Reads

Blockade of YAP alleviates hepatic fibrosis through accelerating apoptosis and reversion of activated hepatic stellate cells.

Mol Immunol 2019 Mar 11;107:29-40. Epub 2019 Jan 11.

The Key Laboratory of Major Autoimmune Diseases, Anhui Province, Anhui Institute of Innovative Drugs, School of Pharmacy, Anhui Medical University, Hefei, China; The Key Laboratory of Anti-inflammatory of Immune Medicines, Ministry of Education, Hefei, China; Institute for Liver Diseases of Anhui Medical University, Hefei, China. Electronic address:

Yes-associated protein (YAP) is a significant downstream protein in the Hippo signaling pathway with important functions in cell proliferation, apoptosis, invasion and migration. YAP also plays a role in the progression and development of various liver diseases. In hepatic fibrosis development and reversion, the proliferation and apoptosis of activated hepatic stellate cells (HSCs) play a critical role. Read More

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http://dx.doi.org/10.1016/j.molimm.2019.01.004DOI Listing
March 2019
1 Read
2.973 Impact Factor

ZAP70 deficiency promotes reverse cholesterol transport through MAPK/ERK pathway in Jurkat cell.

Mol Immunol 2019 Mar 10;107:21-28. Epub 2019 Jan 10.

Department of Cardiology, Huiqiao Medical Center, Nanfang Hospital, Southern Medical University, Guangzhou 510515, Guangdong, People's Republic of China. Electronic address:

Background: Lots of studies have demonstrated that immune cells could regulate reverse cholesterol transport (RCT). However, neither T cell receptor (TCR) signalling nor Zeta-chain associated protein 70 (ZAP70) have been demonstrated to be associated with RCT. To investigate this association, we used a ZAP70-deficient Jurkat-derived mutant, P116 cell line, to detect the effect of ZAP70 on RCT and inflammatory response. Read More

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http://dx.doi.org/10.1016/j.molimm.2019.01.001DOI Listing
March 2019
2 Reads

Calreticulin induced endothelial ICAM-1 up-regulation associated with tristetraprolin expression alteration through PI3K/Akt/eNOS/p38 MAPK signaling pathway in rheumatoid arthritis.

Mol Immunol 2019 Mar 9;107:10-20. Epub 2019 Jan 9.

Department of Clinical Immunology, School of Medical Laboratory, Tianjin Medical University, Tianjin 300203, China. Electronic address:

The present study was undertaken to determine whether extracellular calreticulin (CRT) participates in the regulation of ICAM-1in rheumatoid arthritis (RA) and further explore the potential mechanism. Our results showed that ICAM-1 and VCAM-1 levels were positively correlated with CRT levels in RA serum and synovial fluid, respectively. In RA synovial tissue, increased co-expressions of CRT and ICAM-1 in vascular endothelium and perivascular areas and elevated co-location of CRT and VCAM-1 localized predominantly to lining layer were observed compared to those in OA. Read More

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http://dx.doi.org/10.1016/j.molimm.2019.01.005DOI Listing
March 2019
4 Reads

IL-16 regulates macrophage polarization as a target gene of mir-145-3p.

Mol Immunol 2019 Mar 8;107:1-9. Epub 2019 Jan 8.

Department of hematology,department of orthopedics,the First Affiliated Hospital of Kunming Medical University, China.. Electronic address:

Background: Interleukin 16 is an immunomodulatory chemokine that signals through CD4 + T cells, monocytes, macrophages and dendritic cells. Its expression in immune-related cells enhances the antimicrobial effect and inhibits HIV replication in macrophages. However, the role of IL-16 in macrophage polarization is uncertain. Read More

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http://dx.doi.org/10.1016/j.molimm.2018.12.027DOI Listing
March 2019
2 Reads

Comprehensive assessment of T cell receptor β repertoire in Stevens-Johnson syndrome/toxic epidermal necrolysis patients using high-throughput sequencing.

Mol Immunol 2019 Feb 7;106:170-177. Epub 2019 Jan 7.

Department of Dermatology, Huashan Hospital, Fudan University, Shanghai, 200040, China. Electronic address:

Stevens-Johnson syndrome (SJS) /toxic epidermal necrolysis (TEN) are life-threatening severe cutaneous adverse drug reactions characterized by widespread epidermal necrosis. Recent studies have indicated that SJS/TEN is a specific immune reaction regulated by T cells. Certain drug serves as foreign antigens that are presented by major histocompatibility complex (MHC) and recognized by T cell receptors (TCRs), inducing adaptive immune responses. Read More

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http://dx.doi.org/10.1016/j.molimm.2019.01.002DOI Listing
February 2019
6 Reads

The vaccine adjuvant MPLA activates glycolytic metabolism in mouse mDC by a JNK-dependent activation of mTOR-signaling.

Mol Immunol 2019 Feb 9;106:159-169. Epub 2019 Jan 9.

Paul-Ehrlich-Institut, Vice President´s Research Group 1: Molecular Allergology, Langen, Germany. Electronic address:

Introduction: The detoxified TLR4-ligand MPLA is a successfully used adjuvant in clinically approved vaccines. However, its capacity to activate glycolytic metabolism in mDC and the influence of MPLA-induced metabolic changes on cytokine secretion are unknown.

Aim: To analyze the capacity of MPLA to activate mDC metabolism and the mechanisms contributing to MPLA-induced metabolism activation and cytokine secretion. Read More

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http://dx.doi.org/10.1016/j.molimm.2018.12.029DOI Listing
February 2019
9 Reads

The protective effect of miR-377 inhibitor against renal ischemia-reperfusion injury through inhibition of inflammation and oxidative stress via a VEGF-dependent mechanism in mice.

Mol Immunol 2019 Feb 3;106:153-158. Epub 2019 Jan 3.

Department of Anesthesiology, Cangzhou Central Hospital, Teaching Hospital of Tianjin Medical University, Cangzhou 061000, Hebei, China.

MicroRNAs (miRNAs) play important roles in kidney development and maintenance of kidney physiological functions. MiR-377 has been reported to regulate inflammation in cardiac and cerebral ischemia. However, it remains unclear whether it has a similar function in renal ischemia/reperfusion (I/R). Read More

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http://dx.doi.org/10.1016/j.molimm.2018.12.028DOI Listing
February 2019
1 Read

Electroacupuncture at ST-36 ameliorates DSS-induced acute colitis via regulating macrophage polarization induced by suppressing NLRP3/IL-1β and promoting Nrf2/HO-1.

Mol Immunol 2019 Feb 2;106:143-152. Epub 2019 Jan 2.

Department of Gastroenterology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, China. Electronic address:

Background: Electroacupuncture (EA) at ST-36 can attenuate acute experimental colitis, but the mechanisms are unclear. We investigated the role of macrophages in the anti-inflammatory effects of EA and its molecular mechanisms.

Methods: Male C57BL/6 mice were randomized into five groups: normal control, dextran sulfate sodium (DSS)-induced acute colitis (DSS), DSS with sham EA (SEA), DSS with high-frequency EA (HEA) and DSS with low-frequency EA (LEA). Read More

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http://dx.doi.org/10.1016/j.molimm.2018.12.023DOI Listing
February 2019
7 Reads

Widespread positive selection on cetacean TLR extracellular domain.

Mol Immunol 2019 Feb 29;106:135-142. Epub 2018 Dec 29.

Jiangsu Key Laboratory for Biodiversity and Biotechnology, College of Life Sciences, Nanjing Normal University, 1 Wenyuan Road, Nanjing, 210023, China. Electronic address:

Toll like receptors (TLRs), key members of innate immune system, can recognize a wide diversity of pathogens and initiate both innate and adaptive immune responses in vertebrate. Cetaceans must have faced new challenges of pathogens when their terrestrial relatives transitioned from the terrestrial to aquatic environment. Here, we sequenced the extracellular domain (ECD) of 10 TLRs in cetacean lineages because this region involved in the recognition of pathogens. Read More

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http://dx.doi.org/10.1016/j.molimm.2018.12.022DOI Listing
February 2019

Cross-allergenicity of crustacean and the edible insect Gryllus bimaculatus in patients with shrimp allergy.

Mol Immunol 2019 Feb 28;106:127-134. Epub 2018 Dec 28.

Division of Bioscience and Bioindustry, Tokushima University, Tokushima, Japan. Electronic address:

Food scarcity is a serious problem for many developing as well as developed countries. Edible insects have attracted attention recently as a novel food source. Crickets are especially high in nutritional value and easy to breed and harvest. Read More

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http://dx.doi.org/10.1016/j.molimm.2018.12.015DOI Listing
February 2019

TIMP-3 suppression induces choroidal neovascularization by moderating the polarization of macrophages in age-related macular degeneration.

Mol Immunol 2019 Feb 27;106:119-126. Epub 2018 Dec 27.

Department of Geriatrics, Qilu Hospital of Shandong University, No. 107, Wenhuaxi Road, Jinan, 250012, China. Electronic address:

Purpose: To investigate the role of tissue inhibitor of metalloproteinases-3 (TIMP-3) as a key moderator of macrophage polarization in choroidal neovascularization (CNV) lesions of model mice and in bone marrow-derived macrophage (BMDM).

Method: We used siR-TIMP-3 to transfect BMDM and gave an intravitreal injection of siR-TIMP-3 to laser-induced CNV mice model, real time-PCR and western blot were applied for detecting the expressions of TIMP-3 and macrophages' biomarker. Besides, CNV lesions in different treatment groups of animal model were examined by the optical coherence tomography angiography (OCTA). Read More

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http://dx.doi.org/10.1016/j.molimm.2018.12.026DOI Listing
February 2019
2 Reads

Immunogenicity and protective efficacy of a new Leishmania hypothetical protein applied as a DNA vaccine or in a recombinant form against Leishmania infantum infection.

Mol Immunol 2019 Feb 27;106:108-118. Epub 2018 Dec 27.

Programa de Pós-Graduação em Ciências da Saúde: Infectologia e Medicina Tropical, Faculdade de Medicina, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil; Departamento de Patologia Clínica, COLTEC, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil. Electronic address:

Vaccination is one the most important strategies for the prevention of visceral leishmaniasis (VL). In the current study, a new Leishmania hypothetical protein, LiHyP, which was previously showed as antigenic in an immunoproteomic search in canine VL, was evaluated regarding its immunogenicity and protective efficacy against Leishmania infantum infection. The effects of the immunization using LiHyP were evaluated when administered as a DNA plasmid (DNA LiHyP) or recombinant protein (rLiHyP) associated with saponin. Read More

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http://dx.doi.org/10.1016/j.molimm.2018.12.025DOI Listing
February 2019

A time-course study of gene expression and antibody repertoire at early time post vaccination of Atlantic salmon.

Mol Immunol 2019 Feb 26;106:99-107. Epub 2018 Dec 26.

Nofima AS, Norwegian Institute of Food, Fisheries & Aquaculture Research, Ås, Norway. Electronic address:

The majority of studies of vaccine responses in Atlantic salmon have focused on several weeks after vaccination, and employed a limited number of marker genes. In this study, novel techniques were used to examine a broad panel of expressed genes and antibody repertoire of Atlantic salmon following vaccination. Salmon parr were vaccinated with a multivalent oil-based vaccine, and blood plasma and head kidney were sampled at several time-points between 0-35 days post vaccination. Read More

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http://dx.doi.org/10.1016/j.molimm.2018.12.018DOI Listing
February 2019

CpG oligodeoxynucleotide-specific duck TLR21 mediates activation of NF-κB signaling pathway and plays an important role in the host defence of DPV infection.

Mol Immunol 2019 Feb 26;106:87-98. Epub 2018 Dec 26.

Research Centre of Avian Disease, College of Veterinary Medicine of Sichuan Agricultural University, Chengdu, Sichuan, 611130, PR China; Institute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu, Sichuan, 611130, PR China; Key Laboratory of Animal Disease and Human Health of Sichuan Province, Chengdu, Sichuan, 611130, PR China. Electronic address:

TLR21 can recognize unmethylated cytosine-phosphate-guanine oligodeoxynucleotides (CpG-ODN) and activates NF-κB immune signaling pathway. However, the function of TLR21 in duck remains largely unclear. Here, the complete duck TLR21 (duTLR21) cDNA was cloned from Cherry Valley duck for the first time, and its immune response was preliminarily studied. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S01615890183104
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http://dx.doi.org/10.1016/j.molimm.2018.12.019DOI Listing
February 2019
8 Reads
2.973 Impact Factor

Dysregulation of T cell immunoglobulin and mucin domain 3 (TIM-3) signaling in peripheral immune cells is associated with immune dysfunction in autistic children.

Mol Immunol 2019 Feb 24;106:77-86. Epub 2018 Dec 24.

Department of Pharmacology and Toxicology, College of Pharmacy, King Saud University, Riyadh, Saudi Arabia; Department of Pharmacology and Toxicology, College of Pharmacy, Al-Azhar University, Cairo, Egypt.

Evidence suggests that immune dysregulation is associated with autism spectrum disorder (ASD). T cell immunoglobulin and mucin domain-3 (TIM-3) has a critical role in several inflammatory disorders; however, the role of TIM-3 signaling has not been demonstrated in ASD. In the present study, we assessed the role of TIM-3 signaling in children with ASD. Read More

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http://dx.doi.org/10.1016/j.molimm.2018.12.020DOI Listing
February 2019
1 Read

Peptidoglycan recognition proteins in insect immunity.

Mol Immunol 2019 Feb 24;106:69-76. Epub 2018 Dec 24.

Institute of Life Sciences, Jiangsu University, Zhenjiang, Jiangsu, PR China. Electronic address:

Insects lack an acquired immune system and rely solely on the innate immune system to combat microbial infection. The innate immunity of insects mainly depends on the interaction between the host's pattern recognition receptor (PRR) and pathogen-associated molecular pattern (PAMP). The peptidoglycan recognition proteins (PGRPs) family is the most important pattern recognition receptor (PRR) for insects. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S01615890183090
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http://dx.doi.org/10.1016/j.molimm.2018.12.021DOI Listing
February 2019
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Human antibodies eluted from ligand-free Sepharose capable of binding bacterial polysaccharides and sulfated glycans.

Mol Immunol 2019 Feb 22;106:63-68. Epub 2018 Dec 22.

Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, 16/10 ul. Miklukho-Maklaya, Moscow, 117997, Russian Federation; School of Engineering, Computer & Mathematical Sciences, Auckland University of Technology, Auckland 1010, New Zealand. Electronic address:

Sepharose matrix without immobilized ligands binds antibodies from human blood serum or immunoglobulin preparations. The eluted antibodies bind bacterial polysaccharides having no structural similarity to agarose (Sepharose is a cross-linked polysaccharide agarose) with a high affinity. It is concluded that the identified antibodies are capable of recognizing spatial rather than linear epitopes of bacterial polysaccharides. Read More

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http://dx.doi.org/10.1016/j.molimm.2018.12.011DOI Listing
February 2019

USP25 promotes endotoxin tolerance via suppressing K48-linked ubiquitination and degradation of TRAF3 in Kupffer cells.

Mol Immunol 2019 Feb 19;106:53-62. Epub 2018 Dec 19.

Department of Hepatobiliary Surgery, he Second Affiliated Hospital of Chongqing Medical University, Chongqing, 400010, China. Electronic address:

The inhibition of tumor necrosis factor receptor-associated factor 3 (TRAF3) degradation induces endotoxin tolerance (ET) in macrophages. However, the mechanisms leading to TRAF3 inhibition by ET are largely unknown. Here, we found that ubiquitin-specific peptidase 25 (USP25), a deubiquitinating enzyme (DUB), interacted with TRAF3 and stabilized ET in Kupffer cells (KCs). Read More

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http://dx.doi.org/10.1016/j.molimm.2018.12.017DOI Listing
February 2019

Design, biological activity and signaling pathway of bovine consensus omega interferon expressed in Pichia pastoris.

Mol Immunol 2019 Feb 18;106:46-52. Epub 2018 Dec 18.

Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Jilin University, Jilin, Changchun, 130062, PR China; Jiangsu Co-Innovation Center for the Prevention and Control of Important Animal Infectious Disease and Zoonose,Yangzhou University,Yangzhou, 225009,PR China. Electronic address:

The bovine IFN-ω (BoIFN-ω) multigene family is located on chromosome 8, which has 14 potential functional genes and 10 pseudogenes. After aligning 14 BoIFN-ω subtypes and assigning the most frequently occurring amino acids in each position, one artificial consensus BoIFN-ω (CoBoIFN-ω) gene was designed, optimized and synthesized. Then, CoBoIFN-ω was expressed in Pichia pastoris, which was demonstrated to have 3. Read More

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http://dx.doi.org/10.1016/j.molimm.2018.12.004DOI Listing
February 2019

Molecular variants of Leishmania (Viannia) braziliensis trigger distinct patterns of cytokines and chemokines expression in golden hamster.

Mol Immunol 2019 Feb 18;106:36-45. Epub 2018 Dec 18.

Grupo de Estudos em Leishmanioses, Instituto René Rachou, Fundação Oswaldo Cruz (FIOCRUZ), Av. Augusto de Lima, 1715 Barro Preto, CEP 30190-002, Belo Horizonte, Minas Gerais, Brazil.

Cutaneous leishmaniasis (CL) mainly caused by Leishmania braziliensis is a chronic inflammatory disease widely spread in Brazil. Genetic variant strains of this parasite have been associated with atypical clinical manifestations of CL in an endemic area in Brazil. Furthermore, these strains have presented distinct biological behaviors in golden hamster, suggesting differential activation of the immune response. Read More

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http://dx.doi.org/10.1016/j.molimm.2018.12.013DOI Listing
February 2019
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The neurokinin-1 receptor antagonist aprepitant ameliorates oxidized LDL-induced endothelial dysfunction via KLF2.

Mol Immunol 2019 Feb 18;106:29-35. Epub 2018 Dec 18.

Department Obstetrics and Gynecology, Affiliated Hospital of North Sichuan Medical College, Nanchong, Sichuang, 637000, China. Electronic address:

Atherosclerosis is the main cause of many cardiovascular diseases. Endothelial dysfunction is recognized as an early event in the development of atherosclerosis. Many drugs have been studied to mitigate hyperlipidemia-induced endothelial injury. Read More

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http://dx.doi.org/10.1016/j.molimm.2018.12.009DOI Listing
February 2019

IL-17 production by NKG2D-expressing CD56+ T cells in type 2 diabetes.

Mol Immunol 2019 Feb 18;106:22-28. Epub 2018 Dec 18.

The Centre for Research and Development of Medical Diagnostic Laboratories (CMDL), Faculty of Associated Medical Sciences, Khon Kaen University, Khon Kaen, 40002, Thailand; Department of Clinical Immunology and Transfusion Sciences, Faculty of Associated Medical Sciences, Khon Kaen University, Khon Kaen, 40002, Thailand. Electronic address:

T cells expressing CD56 (identified as CD3+CD56+) play a potential role in activation or regulation of other immune cells by secreting various cytokines. We hypothesized that these cells expressing the natural group 2, member D (NKG2D) could produce high levels of interleukin (IL)-17 in type 2 diabetes (T2D). CD56 + T cells expressing NKG2D of T2D patients, particularly in poor glycemic control (PC) predominantly produced higher IL-17 compared to the NKG2D negative population. Read More

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http://dx.doi.org/10.1016/j.molimm.2018.12.008DOI Listing
February 2019

Chemerin-activated functions of CMKLR1 are regulated by G protein-coupled receptor kinase 6 (GRK6) and β-arrestin 2 in inflammatory macrophages.

Mol Immunol 2019 Feb 18;106:12-21. Epub 2018 Dec 18.

Thurston Arthritis Research Center and the Department of Medicine, Division of Rheumatology, Allergy, and Immunology, University of North Carolina, Chapel Hill, NC 27599, United States; Department of Cell Biology and Physiology, University of North Carolina, Chapel Hill, NC 27599, United States. Electronic address:

Chemerin receptor (CMKLR1) is a G protein-coupled receptor (GPCR) implicated in macrophage-mediated inflammation and in several forms of human arthritis. Analogous to other GPCR, CMKLR1 is likely regulated by G protein-coupled receptor kinase (GRK) phosphorylation of intracellular domains in an activation-dependent manner, which leads to recruitment and termination of intracellular signaling via desensitization and internalization of the receptor. The ubiquitously expressed GRK family members include GRK2, GRK3, GRK5, and GRK6, but it is unknown which GRK regulates CMKLR1 cellular and signaling functions. Read More

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http://dx.doi.org/10.1016/j.molimm.2018.12.016DOI Listing
February 2019

Antibody responses to chimeric peptides derived from parasite antigens in mice and other animal species.

Mol Immunol 2019 Feb 18;106:1-11. Epub 2018 Dec 18.

Laboratorio de Parasitología, Facultad de Farmacia, Universidad de Santiago de Compostela, 15782 Santiago de Compostela, Spain. Electronic address:

Peptide vaccines constitute an interesting alternative to classical vaccines due to the possibility of selecting specific epitopes, easy of production and safety. However, an inadequate design may render these peptides poorly immunogenic or lead to undesirable outcomes (e.g. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S01615890183057
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http://dx.doi.org/10.1016/j.molimm.2018.11.019DOI Listing
February 2019
5 Reads

Evaluation of Epstein-Barr virus-specific antibodies in Cypriot multiple sclerosis patients.

Mol Immunol 2019 Jan 15;105:270-275. Epub 2018 Dec 15.

Cyprus School of Molecular Medicine, The Cyprus Institute of Neurology and Genetics, Nicosia, Cyprus; Department of Molecular Virology, The Cyprus Institute of Neurology and Genetics, Nicosia, Cyprus.

Multiple Sclerosis (MS) is a chronic, demyelinating, inflammatory disease of the central nervous system (CNS) with a strong autoimmune component. Several genetic and environmental factors have been suggested to contribute in MS. The Epstein-Barr virus (EBV) is one pathogenic candidate proposed to be involved in the onset of MS and/or induction of subsequent exacerbations. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S01615890183062
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http://dx.doi.org/10.1016/j.molimm.2018.12.010DOI Listing
January 2019
8 Reads

Antifungal activities of surfactant protein D in an environment closely mimicking the lung lining.

Mol Immunol 2019 Jan 15;105:260-269. Epub 2018 Dec 15.

Department of Infectious Diseases and Immunology, Division Molecular Host Defence, Faculty of Veterinary Medicine, Utrecht University, Utrecht, the Netherlands. Electronic address:

At the lung lining innate defenses protect our lungs against inhaled fungal cells that could pose a threat to our health. These defenses are comprised of mucociliary clearance, soluble effector molecules and roaming phagocytic cells, such as macrophages and neutrophils. How important each of these defenses is during fungal clearance depends on the specific fungal pathogen in question and on the stage of infection. Read More

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http://dx.doi.org/10.1016/j.molimm.2018.12.003DOI Listing
January 2019

Next-generation sequencing reveals new insights about gene usage and CDR-H3 composition in the horse antibody repertoire.

Mol Immunol 2019 Jan 15;105:251-259. Epub 2018 Dec 15.

Laboratory of Synthetic Biology and Biomimetics, Departamento de Bioquímica e Imunologia, Instituto de Ciências Biológicas - ICB, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil. Electronic address:

Horse serum antibodies have been used for greater than a century for the treatment and prophylaxis of infectious diseases and envenomations. Little is known, however, about the immunogenetic diversity that produces horse serum antibodies. Here, we employed next-generation sequencing for a first-in-kind comprehensive analysis of the equine B-cell repertoire. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S01615890183066
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http://dx.doi.org/10.1016/j.molimm.2018.11.017DOI Listing
January 2019

IL-17A contributes to myocardial ischemic injury by activating NLRP3 inflammasome in macrophages through AMPKα/p38MAPK/ERK1/2 signal pathway in mice.

Mol Immunol 2019 Jan 15;105:240-250. Epub 2018 Dec 15.

Department of Cardiovascular Medicine, The First Affiliated Hospital, Xi'an Jiaotong University, Xi'an, Shaanxi, PR China; Key Laboratory of Molecular Cardiology, Shaanxi Province, Xi'an, Shaanxi, PR China. Electronic address:

Background: Acute myocardial infarction (AMI) is followed by an acute inflammation involving inflammasome activation, thereby inducing cardiac dysfunction. Interleukin-17A (IL-17A) involves in many inflammatory diseases, but its roles in inflammation following AMI are still obscure. The aim of this study is to investigate the roles of IL-17A in the inflammatory response following AMI and its underlying mechanisms. Read More

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http://dx.doi.org/10.1016/j.molimm.2018.12.014DOI Listing
January 2019
2.973 Impact Factor

Chlamydia trachomatis ct143 stimulates secretion of proinflammatory cytokines via activating the p38/MAPK signal pathway in THP-1 cells.

Mol Immunol 2019 Jan 13;105:233-239. Epub 2018 Dec 13.

Department of Immunology, Xiangya School of Medicine, Central South University, Changsha, 410078, China.

Chlamydia trachomatis (Ct) infections can cause bacterial sexually-transmitted and preventable blindness. The Ct infections induced excessive cytokines generation which attributed to pathologic changes in host cells. However, the precise mechanisms of Ct-induced cytokines production are still unclear. Read More

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http://dx.doi.org/10.1016/j.molimm.2018.12.007DOI Listing
January 2019
1 Read