149,497 results match your criteria Molecular Diagnosis & Therapy[Journal]


[Application of stretched exponential diffusion-weighted imaging model in quantitative diagnosis of nonalcoholic fatty liver disease: in a rabbit model].

Zhonghua Yi Xue Za Zhi 2019 Feb;99(7):500-504

Department of Radiology, Northern Jiangsu People's Hospital, Clinical Medical School of Yangzhou University, Yangzhou 225001, China.

To evaluate the feasibility of diffusion parameters obtained from mono-exponential and stretched exponential diffusion-weighted imaging models in staging of nonalcoholic fatty liver disease and compare the diagnosis ability of nonalcoholic steatohepatitis. Thirty-two 12 -week-old male New Zealand rabbits, about 2.5 kg of each, were randomly divided into five groups. Read More

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http://dx.doi.org/10.3760/cma.j.issn.0376-2491.2019.07.005DOI Listing
February 2019

[Activation of GPER inhibts proliferation and autophagy in prostatic epithelial cells].

Zhonghua Yi Xue Za Zhi 2019 Feb;99(6):419-422

Department of Urology, the First Affiliated Hospital of Guangzhou Medical College, Guangdong Key Laboratory of Urology, Guangzhou 510230, China.

To investigate the effect of G protein-coupled estrogen receptor (GPER) on proliferation and autophagy in prostatic epithelial cells. Cell Counting Kit-8 (CCK8) assay was used to observe the growth of BPH-1 and RWPE-1 cells after treating with or not with estradiol or GPER selective agonist G1. Autophagy was quantified with Western blot and Cyto-ID autophagy detection kit after treating with estradiol, G1 or both G1 and G15 in the same cells. Read More

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http://dx.doi.org/10.3760/cma.j.issn.0376-2491.2019.06.007DOI Listing
February 2019

Cellular origin of microRNA-371a-3p in healthy males based on systematic urogenital tract tissue evaluation.

Andrology 2019 Feb 20. Epub 2019 Feb 20.

Pathology (LEPO), Erasmus MC Cancer Institute, University Medical Center, Rotterdam, The Netherlands.

Background: The microRNA-371a-3p (miR-371a-3p) has been reported to be an informative liquid biopsy (serum and plasma) molecular biomarker for both diagnosis and follow-up of patients with a malignant (testicular) germ cell tumor ((T)GCT). It is expressed in all histological cancer elements, with the exception of mature teratoma. However, normal testis, semen, and serum of males with a disrupted testicular integrity without a TGCT may contain miR-371a-3p levels above threshold, of which the cellular origin is unknown. Read More

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http://dx.doi.org/10.1111/andr.12595DOI Listing
February 2019

The Biospecimen Preanalytical Variables Program: A Multiassay Comparison of Effects of Delay to Fixation and Fixation Duration on Nucleic Acid Quality.

Arch Pathol Lab Med 2019 Feb 20. Epub 2019 Feb 20.

From Biorepositories and Biospecimen Research Branch, Cancer Diagnosis Program, Division of Cancer Treatment and Diagnosis, National Cancer Institute, Bethesda, Maryland (Drs Carithers, Guan, Odeh, Sachs, Branton, and Moore);Leidos Biomedical Research, Inc., Frederick National Laboratory for Cancer Research, Frederick, Maryland (Drs Agarwal, Barcus, Lih, Williams, Sobin, Roche, and Mr Soria);Preferred Solutions Group, Preferred Scientific Group, Washington, District of Columbia (Dr Engel);Kelly Government Solutions, Kelly Services, Rockville, Maryland (Dr Greytak and Mr Fombonne);the Department of Pathology, University of New Mexico, Albuquerque (Dr Bocklage);the Department of Pathology and Laboratory Medicine, Boston Medical Center, Boston, Massachusetts (Dr Andry and Ms Duffy);the Department of Pathology and Laboratory Medicine, Emory University, Winship Cancer Institute, Atlanta, Georgia (Dr Sica);the Department of Pathology, University of Pittsburg, Pittsburgh, Pennsylvania (Dr Dhir);and Van Andel Research Institute, Pathology and Biorepository Core, VARI Core Technologies and Services, Grand Rapids, Michigan (Dr Jewell).

Context.—: Despite widespread use of formalin-fixed, paraffin-embedded (FFPE) tissue in clinical and research settings, potential effects of variable tissue processing remain largely unknown.

Objective. Read More

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http://dx.doi.org/10.5858/arpa.2018-0172-OADOI Listing
February 2019

Pre-analytical parameters associated with unsuccessful karyotyping in myeloid neoplasm: a study of 421 samples.

Braz J Med Biol Res 2019 Feb 14;52(2):e8194. Epub 2019 Feb 14.

Hospital Israelita Albert Einstein, São Paulo, SP, Brasil.

Cytogenetics is essential in myeloid neoplasms (MN) and pre-analytical variables are important for karyotyping. We assessed the relationship between pre-analytical variables (time from collection to sample processing, material type, sample cellularity, and diagnosis) and failures of karyotyping. Bone marrow (BM, n=352) and peripheral blood (PB, n=69) samples were analyzed from acute myeloid leukemia (n=113), myelodysplastic syndromes (n=73), myelodysplastic syndromes/myeloproliferative neoplasms (n=17), myeloproliferative neoplasms (n=137), and other with conclusive diagnosis (n=6), and reactive disorders/no conclusive diagnosis (n=75). Read More

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http://dx.doi.org/10.1590/1414-431X20188194DOI Listing
February 2019

Neonatal pertussis diagnosis: low procalcitonin level and high lymphocyte count are able to discriminate pertussis from bacterial and viral infections.

New Microbiol 2019 Feb 20;42(1). Epub 2019 Feb 20.

Dipartimento di Pediatria Sistematica, Pediatria 1, AORN "Santobono-Pausilipon", Napoli, Italy.

Pertussis is quite frequent and severe among infants; therefore, rapid diagnosis and timely targeted therapy are essential. Although a molecular test for etiological diagnosis is now available, it may not be available everywhere, and therefore adjunctive diagnostic tests are still useful for presumptive diagnosis. We describe the use of procalcitonin (PCT) and lymphocyte count to discriminate among pertussis, bacterial and viral infections. Read More

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February 2019

Hybridization chain reaction-based nanoprobe for cancer cell recognition and amplified photodynamic therapy.

Chem Commun (Camb) 2019 Feb 20. Epub 2019 Feb 20.

Molecular Science and Biomedicine Laboratory, State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, P. R. China.

Precision diagnosis and effective treatment are the cores of early cancer therapy. Here, for the first time, we report a hybridization chain reaction-based nanoprobe for selective and sensitive cancer cell recognition and amplified photodynamic therapy. Read More

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http://dx.doi.org/10.1039/c8cc10074hDOI Listing
February 2019

Matter, energy and information network of a graphene-peptide-based fluorescent sensing system for molecular logic computing, detection and imaging of cancer stem cell marker CD133 in cells and tumor tissues.

Analyst 2019 Feb 20. Epub 2019 Feb 20.

State Key Laboratory of Developmental Biology of Freshwater Fish, Hunan Provincial Key Laboratory of Microbial Molecular Biology, College of Life Science, Hunan Normal University, Changsha 410081, P. R. China.

Tumorigenesis, metastasis, and the recurrence of cancer, which may result from the abnormal presence or activation of cancer stem cells (CSCs), are involved in disorders of exchanged matter (biomarkers), energy and information in living organisms. Rapid and sensitive detection and imaging of CSC biomarkers (such as CD133) are helpful for early diagnosis and therapeutic evaluation of tumors. Recently, a preliminary exploration of a few affinity molecules (like peptide-based probes) has just begun for chemical measurements and imaging of CSC biomarker CD133. Read More

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http://dx.doi.org/10.1039/c8an02115eDOI Listing
February 2019

Contribution of In Vivo and In Vitro Testing for The Diagnosis of Local Allergic Rhinitis.

J Investig Allergol Clin Immunol 2019 Feb;29(1):46-48

Serviço de Imunoalergologia, Centro Hospitalar de Lisboa Norte, Lisbon, Portugal.

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http://dx.doi.org/10.18176/jiaci.0321DOI Listing
February 2019

Fumarate hydratase deficient renal cell carcinoma: Chromosomal numerical aberration analysis of 12 cases.

Ann Diagn Pathol 2019 Feb 10;39:63-68. Epub 2019 Feb 10.

Department of Pathology, Charles University in Prague, Faculty of Medicine in Plzeň, University Hospital Plzen, Pilsen, Czech Republic. Electronic address:

Hereditary leiomyomatosis and renal cell carcinoma-associated renal cell carcinoma (HLRCC)/fumarate hydratase deficient renal cell carcinoma (FHRCC) is defined by molecular genetic changes (mutation/LOH in fumarate hydratase (FH) gene). We investigated chromosomal numerical aberration pattern (CNV) in FHRCC/HLRCC using array comparative genomic hybridization analysis and low pass whole genome sequencing. Genetic analysis was successfully completed in 12 tumors. Read More

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http://dx.doi.org/10.1016/j.anndiagpath.2019.02.008DOI Listing
February 2019

Effect of intranasal oxytocin on alcohol withdrawal syndrome: A randomized placebo-controlled double-blind clinical trial.

Drug Alcohol Depend 2019 Feb 13;197:95-101. Epub 2019 Feb 13.

Department of Clinical and Molecular Medicine, Faculty of Medicine and Health Sciences, Norwegian University of Science and Technology - NTNU, Trondheim, Norway; Department of Clinical Pharmacology, St. Olav University Hospital, Trondheim, Norway.

Background: In a pilot study, intranasal oxytocin was demonstrated to reduce the benzodiazepine dose needed to relieve withdrawal symptoms during alcohol detoxification. The aim of the present study was to compare the effect of oxytocin and placebo during a three-day period of alcohol detoxification at an addiction treatment center in Norway.

Methods: Randomized, double-blind, placebo-controlled trial with 40 patients fulfilling criteria for ICD-10 diagnosis of alcohol dependence (F10. Read More

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http://dx.doi.org/10.1016/j.drugalcdep.2019.01.003DOI Listing
February 2019

Assembly of upconversion nanophotosensitizer in vivo to achieve scatheless real-time imaging and selective photodynamic therapy.

Biomaterials 2019 Feb 13;201:33-41. Epub 2019 Feb 13.

Van 't Hoff Institute for Molecular Sciences. University of Amsterdam, Science Park 904, 1098 XH, Amsterdam, the Netherlands. Electronic address:

A perfect "off" to "on" switch of the therapeutic function is very important to minimize the phototoxicity of nanoplatforms assisted imaging-guided photodynamic therapy (PDT) of cancer. Current approaches rely on preloaded photosensitizers, where the off/on state of PDT is regulated by the sensitizing light of photosensitizers. However, the photoactivities inevitably occur when imaging/diagnosis or exposure to sunlight, etc. Read More

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http://dx.doi.org/10.1016/j.biomaterials.2019.02.015DOI Listing
February 2019

Novel ELISA for thrombospondin type 1 domain-containing 7A autoantibodies in membranous nephropathy.

Kidney Int 2019 Mar;95(3):666-679

Université Côte d'Azur, Centre National de la Recherche Scientifique, Institut de Pharmacologie Moléculaire et Cellulaire, UMR7275 Valbonne Sophia Antipolis, France. Electronic address:

Autoantibodies against phospholipase A2 receptor 1 (PLA2R1) and thrombospondin type 1 domain-containing 7A (THSD7A) are emerging as biomarkers to classify membranous nephropathy (MN) and to predict outcome or response to treatment. Anti-THSD7A autoantibodies are detected by Western blot and indirect immunofluorescence test (IIFT). Here, we developed a sensitive enzyme-linked immunosorbent assay (ELISA) optimized for quantitative detection of anti-THSD7A autoantibodies. Read More

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http://dx.doi.org/10.1016/j.kint.2018.10.024DOI Listing

Prognostic risk model construction and molecular marker identification in glioblastoma multiforme based on mRNA/microRNA/long non-coding RNA analysis using random survival forest method.

Authors:
H Wang D Liu J Yang

Neoplasma 2019 Feb 17;2019. Epub 2019 Feb 17.

Department of Neurosurgery, Beijing Luhe Hospital, Capital Medical University, Beijing, China.

We aim to identify novel molecular signatures for prognosis prediction of glioblastoma multiforme (GBM). The expression and microarray data of GBM were downloaded from The Cancer Genome Atlas (TCGA) and the Gene Expression Omnibus (GEO). Differentially expressed mRNAs, microRNAs (miRNAs) and long non-coding RNAs (lncRNAs) between GBM and normal samples were identified by differential expression analysis using Bayesian T-test. Read More

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http://dx.doi.org/10.4149/neo_2018_181008N746DOI Listing
February 2019

The spectrum of genetic variants and phenotypic features of Southeast Asian patients with Noonan syndrome.

Mol Genet Genomic Med 2019 Feb 19:e581. Epub 2019 Feb 19.

Genetics Service, Department of Paediatrics, KK Women's & Children's Hospital, Singapore.

Background: Noonan syndrome (NS) is an autosomal dominant disorder that belongs to a group of developmental disorders called RASopathies with overlapping features and multiple causative genes. The aim of the study was to identify mutations underlying this disorder in patients from Southeast Asia and characterize their clinical presentations.

Methods: Patients were identified from the hospital's Genetics clinics after assessment by attending clinical geneticists. Read More

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http://dx.doi.org/10.1002/mgg3.581DOI Listing
February 2019

Lymphoma diagnostics: getting more from less.

Br J Haematol 2019 Feb 19. Epub 2019 Feb 19.

Department of Pathology, University of Utah and ARUP Laboratories, Salt Lake City, UT, USA.

In the modern era, clinicians and pathologists increasingly make challenging diagnoses in patients with suspected lymphoma using minimal amounts of diagnostic material. The increase in utilization of minimally invasive procedures, such as fine needle aspiration or needle core biopsies, lead to challenges in our ability to make accurate histopathological assessments of disease, including the integration of new diagnostic and prognostic testing, with smaller amounts of material. The trend towards minimally invasive diagnostics is also often in conflicting interest with researchers seeking to study tissue specimens to better understand the biology and genetics of these diseases to move the field forward. Read More

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http://dx.doi.org/10.1111/bjh.15788DOI Listing
February 2019

High-Throughput Profiling of Circulating Antibody Signatures for Stroke Diagnosis Using Small Volumes of Whole Blood.

Neurotherapeutics 2019 Feb 19. Epub 2019 Feb 19.

Valtari Bio Incorporated, Morgantown, West Virginia, USA.

Accurate stroke recognition during triage can streamline care and afford patients earlier access to life-saving interventions. However, the tools currently available to clinicians for prehospital and early in-hospital identification of stroke are limited. The peripheral immune system is intricately involved in stroke pathology and thus may be targetable for the development of immunodiagnostics. Read More

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http://link.springer.com/10.1007/s13311-019-00720-9
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http://dx.doi.org/10.1007/s13311-019-00720-9DOI Listing
February 2019
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Tear-film-oriented diagnosis for dry eye.

Jpn J Ophthalmol 2019 Feb 19. Epub 2019 Feb 19.

Department of Optics and Spectroscopy, Faculty of Physics, St. Kliment Ohridski University of Sofia, Sofia, Bulgaria.

Tear-film (TF) stability protects the ocular surface epithelium from desiccation and is ensured via cooperation between the ocular surface components including constituents of the TF and ocular surface epithelium. Thus, when those components are insufficient or impaired, the TF breakup that initiates dry eye occurs. Recently, new, commercially available eye drops have appeared in Japan that enable TF stabilization via targeted supplementation of deficient ocular surface components. Read More

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http://dx.doi.org/10.1007/s10384-018-00645-4DOI Listing
February 2019

Campylobacter culture fails to correctly detect Campylobacter in 30% of positive patient stool specimens compared to non-cultural methods.

Eur J Clin Microbiol Infect Dis 2019 Feb 19. Epub 2019 Feb 19.

TriCore Reference Laboratories, Albuquerque, NM, USA.

Campylobacter diagnosis is hampered because many laboratories continue to use traditional stool culture, which is slow and suffers false-negative results. This large multi-site study used a composite reference method consisting of a new FDA-cleared immunoassay and four molecular techniques to compare to culture. Prospectively collected patient fecal specimens (1552) were first preliminarily categorized as positive or negative by traditional culture. Read More

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http://dx.doi.org/10.1007/s10096-019-03499-xDOI Listing
February 2019

Diagnostic utility of a targeted next-generation sequencing gene panel in the clinical suspicion of systemic autoinflammatory diseases: a multi-center study.

Rheumatol Int 2019 Feb 19. Epub 2019 Feb 19.

Department of Molecular Biology-Genetics and Biotechnology, Dr. Orhan Öcalgiray Molecular Biology-Biotechnology and Genetics Research Centre, Graduate School of Science, Engineering and Technology, İstanbul Technical University, Ayazağa Campus, Maslak, 34469, Istanbul, Turkey.

Systemic autoinflammatory diseases (sAIDs) are a heterogeneous group of disorders, having monogenic inherited forms with overlapping clinical manifestations. More than half of patients do not carry any pathogenic variant in formerly associated disease genes. Here, we report a cross-sectional study on targeted Next-Generation Sequencing (NGS) screening in patients with suspected sAIDs to determine the diagnostic utility of genetic screening. Read More

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http://dx.doi.org/10.1007/s00296-019-04252-5DOI Listing
February 2019

Unsupervised identification of disease states from high-dimensional physiological and histopathological profiles.

Mol Syst Biol 2019 Feb 19;15(2):e8636. Epub 2019 Feb 19.

Laboratory of Systems Pharmacology and Department of Systems Biology, Harvard Medical School, Boston, MA, USA.

The liver and kidney in mammals play central roles in protecting the organism from xenobiotics and are at high risk of xenobiotic-induced injury. Xenobiotic-induced tissue injury has been extensively studied from both classical histopathological and biochemical perspectives. Here, we introduce a machine-learning approach to analyze toxicological response. Read More

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http://dx.doi.org/10.15252/msb.20188636DOI Listing
February 2019

Finnish National Esophago-Gastric Cancer Cohort (FINEGO) for studying outcomes after oesophageal and gastric cancer surgery: a protocol for a retrospective, population-based, nationwide cohort study in Finland.

BMJ Open 2019 Jan 15;9(1):e024094. Epub 2019 Jan 15.

Department of Gastroenterology and Alimentary Tract Surgery, Tampere University Hospital, Tampere, Finland.

Introduction: Surgery for oesophageal and gastric cancers is associated with high morbidity, mortality and poor quality of life postoperatively. The Finnish National Esophago-Gastric Cancer Cohort has been established with the aim of identifying factors that could contribute to improved outcomes in oesophago-gastric cancer.

Methods And Analysis: All patients with oesophageal and gastric cancer diagnosed in Finland between 1987 and 2015 will be identified from the Finnish national registries. Read More

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http://dx.doi.org/10.1136/bmjopen-2018-024094DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6340442PMC
January 2019

Clinical features, molecular characteristics, and treatments of a Chinese girl with sitosterolemia: A case report and literature review.

J Clin Lipidol 2019 Jan 22. Epub 2019 Jan 22.

Department of Genetics and Endocrinology, Guangzhou Women and Children's Medical Center, Guangzhou Medical University, Guangzhou, Guangdong, China. Electronic address:

Sitosterolemia is a rare autosomal recessive disease characterized by a significant increase in blood plant sterol levels. Clinical manifestations usually include xanthomas, hypercholesterolemia,premature atherosclerosis and hematological abnormalities. We report here a sitosterolemia patient who presented with multiple xanthomas and profound hypercholesterolemia since 3 years old. Read More

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http://dx.doi.org/10.1016/j.jacl.2019.01.007DOI Listing
January 2019

NUT carcinoma in a nutshell: A diagnosis to be considered more frequently.

Pathol Res Pract 2019 Jan 30. Epub 2019 Jan 30.

Institute of Pathology, Heidelberg University Hospital, University of Heidelberg, Im Neuenheimer Feld 224, 69120 Heidelberg, Germany.

NUT carcinoma is a rarely diagnosed, poorly differentiated subtype of squamous cell carcinoma, defined by chromosomal rearrangements of the gene encoding nuclear protein of the testis (NUT). It is characterized by extremely aggressive clinical behavior resulting in a dismal prognosis, with a median survival of 6.7 months. Read More

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http://dx.doi.org/10.1016/j.prp.2019.01.043DOI Listing
January 2019

Smartphone-based fluorescent lateral flow immunoassay platform for highly sensitive point-of-care detection of Zika virus nonstructural protein 1.

Anal Chim Acta 2019 May 23;1055:140-147. Epub 2018 Dec 23.

Beijing Institute of Radiation Medicine, Beijing, 100850, PR China; Beijing Key Laboratory of New Molecular Diagnosis Technologies for Infectious Diseases, Beijing, 100850, PR China. Electronic address:

Simple, inexpensive, and rapid diagnostic tests in low-resource settings with limited laboratory equipment and technical expertise are instrumental in reducing morbidity and mortality from epidemic infectious diseases. We developed a smartphone-based fluorescent lateral flow immunoassay (LFIA) platform for the highly sensitive point-of-care detection of Zika virus nonstructural protein 1 (ZIKV NS1). An attachment was designed and 3D-printed to integrate the smartphone with external optical and electrical components, enabling the miniaturization of the instrument and reduction in cost and complexity. Read More

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http://dx.doi.org/10.1016/j.aca.2018.12.043DOI Listing

Plasmonic metal nanoparticles as efficient mass tags for ion signal amplification and ultrasensitive detection of protein markers.

Anal Chim Acta 2019 May 9;1055:1-6. Epub 2019 Jan 9.

Department of Chemistry, The University of Hong Kong, Pokfulam Road, Hong Kong S.A.R, PR China. Electronic address:

The development of sensitive and specific analytical methods is critical for the discovery of molecular biomarkers, which assists disease diagnosis and understanding biological processes. Herein, a highly sensitive method is developed using antibody-conjugated plasmonic metal nanoparticles for the detection of targeted biomarkers down to low attomole level via coupling of immunoassay techniques with laser ionization mass spectrometry (LI-MS). The conjugated antibodies target specific antigens, while the metal nanoparticles act as mass tags and ion reservoirs for the signal amplification. Read More

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http://dx.doi.org/10.1016/j.aca.2018.12.057DOI Listing

Melanoma arising in a Giant congenital melanocytic nevus: two case reports.

Diagn Pathol 2019 Feb 19;14(1):21. Epub 2019 Feb 19.

Federal State Budgetary Institution, "N.N. Blokhin Medical Research Center of Oncology" of the Ministry of Health of the Russian Federation, 115478 Kashirskoye shosse, 24, Moscow, Russian Federation.

Background: A giant congenital melanocytic nevus (GCMN) is found in 0.1% of live-born infants. If present, the lesion has a chance of about 6% to develop into malignant melanoma. Read More

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http://dx.doi.org/10.1186/s13000-019-0797-1DOI Listing
February 2019

A novel lncRNA MCM3AP-AS1 promotes the growth of hepatocellular carcinoma by targeting miR-194-5p/FOXA1 axis.

Mol Cancer 2019 Feb 19;18(1):28. Epub 2019 Feb 19.

Department of Hepatobiliary Surgery, the First Affiliated Hospital of Xi'an Jiaotong University, 277 Yanta West Road, Xi'an, 710061, Shaanxi Province, China.

Background: Hepatocellular carcinoma (HCC) is the most common malignant liver tumor with poor clinical outcomes. Increasing amount of long non-coding RNAs (lncRNAs) have been revealed to be implicated in the carcinogenesis and progression of HCC. However, the expressions, clinical significances, and roles of most lncRNAs in HCC are still unknown. Read More

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http://dx.doi.org/10.1186/s12943-019-0957-7DOI Listing
February 2019

First Report of Downy Mildew on Greenhouse and Landscape Coleus Caused by a Peronospora sp. in Louisiana and New York.

Plant Dis 2006 Aug;90(8):1111

School of Engineering of Lullier, University of Applied Sciences of Western Switzerland, 1254 Jussy, Switzerland.

In May 2005, two commercial greenhouse flower growers, one in Louisiana (LA) and one in New York (NY), submitted coleus, Solenostemon scutellarioides (L.) Codd, plants for diagnosis after observing stunted growth, inward curling and twisting of leaves, and leaf abscission on multiple cultivars. Downy mildew-like growth was observable with hand lens or a microscope on the abaxial leaf surfaces of affected plants. Read More

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http://dx.doi.org/10.1094/PD-90-1111BDOI Listing

First Report of Asian Soybean Rust Caused by Phakopsora pachyrhizi from Mexico.

Plant Dis 2006 Sep;90(9):1260

USDA, ARS, Systematic Botany and Mycology Laboratory, Beltsville, MD 20705.

Leaves of soybean (Glycine max (L.) Merr.; Fabaceae) cv. Read More

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http://dx.doi.org/10.1094/PD-90-1260BDOI Listing
September 2006

Molecular Detection of Plasmodiophora brassicae, Causal Agent of Clubroot of Crucifers, in Plant and Soil.

Plant Dis 2007 Jan;91(1):80-87

Department of Agricultural, Food and Nutritional Science, University of Alberta, Edmonton, AB T6G 2P5, Canada.

Clubroot of crucifers, caused by Plasmodiophora brassicae, recently has been identified in canola (Brassica napus) fields in Alberta, Canada. An effective strategy for managing the disease is to avoid planting cruciferous crops in P. brassicae-infested soil, because the pathogen produces resting spores that can remain infectious for many years. Read More

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http://dx.doi.org/10.1094/PD-91-0080DOI Listing
January 2007

Host-Parasite Relationships in Fall-Sown Sugar Beets Infected by the Stem and Bulb Nematode, Ditylenchus dipsaci.

Plant Dis 2007 Jan;91(1):71-79

ETSIAM-UCO, and IAS-CSIC.

Stunted growth of fall-sown sugar beets (Beta vulgaris) associated with high incidence of crownroot infections and large soil infestations by Ditylenchus dipsaci were observed at the end of the crop growing season in southern Spain by early June 2005. The largest proportion (75%) of the nematode life-stages in plant and soil was the fourth-stage juvenile. The large number (up to 3,750 nematodes per gram of fresh tissue) of D. Read More

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http://dx.doi.org/10.1094/PD-91-0071DOI Listing
January 2007

A New Potyvirus found in Passiflora incence in Florida.

Authors:
C A Baker L Jones

Plant Dis 2007 Feb;91(2):227

Florida Department of Agriculture and Consumer Services, Division of Plant Industry, Gainesville 32614.

During March of 2004 (Alachua County) and again during February of 2006 (Highlands County), specimens of the plant Passiflora incence (passionfruit) with chlorotic symptoms were submitted to the Division of Plant Industry, Gainesville, FL for diagnosis. Cytoplasmic cylindrical inclusions seen in epidermal strips of plant leaves stained in Luxol brilliant green and calcomine orange but not seen in those stained in Azure A indicated the presence of a potyvirus infection. Leaf dips made for electron microscopy also showed virus particles consistent with a potyvirus infection. Read More

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http://dx.doi.org/10.1094/PDIS-91-2-0227ADOI Listing
February 2007

A multidisciplinary approach to understanding skeletal dysplasias.

Expert Rev Endocrinol Metab 2011 Sep;6(5):731-743

d Department of Child Health, University of Glasgow, Royal Hospital for Sick Children (Yorkhill), Dalnair Street, Glasgow, G3 8SJ, UK.

The skeletal dysplasias are a heterogeneous group of conditions of abnormal cartilage and bone development, resulting in a wide range of phenotypes of variable severity from perinatal lethality to mild short stature. Elucidation of the molecular mechanisms underlying these disorders is allowing us to understand more about the etiology of these conditions and classify them based upon the underlying gene defect. This article will discuss the development of bone and cartilage in relation to these conditions, present a clinical approach to their diagnosis and management, and consider new avenues of therapy. Read More

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http://dx.doi.org/10.1586/eem.11.61DOI Listing
September 2011

Update on the molecular signature of differentiated thyroid cancer: clinical implications and potential opportunities.

Expert Rev Endocrinol Metab 2011 Nov;6(6):819-834

d Division of Endocrinology, Department of Pediatrics, Children's Hospital of Richmond at The Commonwealth University Health System, Medical College of Virginia, Richmond, VA, USA.

With the development and maturation of new technologies, there has been a steady incorporation of powerful new tools into the evaluation and management of thyroid nodules and thyroid cancer. An increasing number of reports on oncogene testing and molecular screening in fine-needle aspiration biopsy samples have been published. However, there remains a paucity of data and consensus on combining both conventional and molecular technologies to determine the diagnosis and/or prognosis of disease. Read More

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http://dx.doi.org/10.1586/eem.11.68DOI Listing
November 2011

Prophylactic thyroidectomy in multiple endocrine neoplasia type 2.

Expert Rev Endocrinol Metab 2010 Nov;5(6):867-874

a Endocrine Practice and Molecular Laboratory, Brückenstr. 21, 69120 Heidelberg, Germany.

Medullary thyroid carcinoma (MTC) is the main component of the autosomal dominant cancer syndrome multiple endocrine neoplasia type 2 (MEN 2). MEN 2 is caused by autosomal dominant gain-of-function mutations in the RET proto-oncogene. In RET-mutation carriers, an age-related progression has been documented from normal C-cells to premalignant C-cell hyperplasia and finally to MTC with or without cervical lymph node metastases. Read More

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http://dx.doi.org/10.1586/eem.10.58DOI Listing
November 2010

Complete androgen insensitivity syndrome: diagnosis and management.

Expert Rev Endocrinol Metab 2009 Nov;4(6):565-573

g Department of Pediatric Surgery and Urology and Centre de Référence des Pathologies Gynécologiques Rares, AP-HP Hôpital Necker Enfants-malades, 149, rue de Sèvres, 75743, Paris Cedex 15, France and Université Paris Descartes, Paris, France.

Complete androgen insensitivity syndrome (CAIS) is an X-linked genetic disorder affecting 46,XY individuals, characterized by the loss of function of the androgen receptor gene resulting in complete peripheral androgen resistance. Patients have a nonambiguous female phenotype with normal female external genitalia. Gonads are undescended testes (either intra-abdominal or inguinal), there is no uterus and the length of the vagina is usually very short. Read More

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http://dx.doi.org/10.1586/eem.09.31DOI Listing
November 2009

Autoimmune hypophysitis: expanding the differential diagnosis to CTLA-4 blockade.

Expert Rev Endocrinol Metab 2009 Nov;4(6):681-698

g Department of Pathology, The Johns Hopkins University School of Medicine, Baltimore, MD, USA Feinstone Department of Molecular Microbiology and Immunology, The Johns Hopkins Bloomberg School of Public Health, Baltimore, MD, USA.

Autoimmune hypophysitis is an increasingly recognized disorder that enters in the differential diagnosis of nonfunctioning pituitary masses. The differential diagnosis of these conditions is challenging because of similar clinical presentations and radiological signs. This review describes the essential features of hypophysitis and the other nonfunctioning pituitary masses. Read More

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http://dx.doi.org/10.1586/eem.09.37DOI Listing
November 2009

First Report of Phakopsora pachyrhizi on Kudzu (Pueraria montana var. lobata) in North Carolina and Increased Incidence of Soybean Rust on Soybean in 2006.

Plant Dis 2007 May;91(5):637

Department of Plant Pathology, North Carolina State University, Raleigh 27695-7616.

Asian soybean rust, caused by Phakopsora pachyrhizi H. Sydow & Sydow, was first detected in the continental United States in soybean (Glycine max (L.) Merr. Read More

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http://dx.doi.org/10.1094/PDIS-91-5-0637ADOI Listing

First Report of the Pale Cyst Nematode, Globodera pallida, in the United States.

Plant Dis 2007 Mar;91(3):325

Nematology Laboratory, USDA-ARS, Beltsville Agricultural Research Center, Beltsville, MD 20705.

In 2006, a cyst nematode was discovered in tare dirt at a potato (Solanum tuberosum) processing facility in eastern Idaho. The nematode was found during a routine survey conducted jointly by the Idaho State Department of Agriculture and the USDA Animal and Plant Health Inspection Service through the Cooperative Agricultural Pest Survey program. Extensive additional sampling from two suspect fields led to the identification of the same nematode in a 45-acre (18. Read More

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http://dx.doi.org/10.1094/PDIS-91-3-0325BDOI Listing

First Report of Tomato yellow leaf curl virus in Venezuela.

Plant Dis 2007 Jun;91(6):768

Instituto Venezolano de Investigaciones Científicas, Centro de Microbiología y Biología Celular, Laboratorio de Biotecnología y Virología Vegetal, Apartado Postal 21827, Caracas 1020-A, Venezuela.

Tomato yellow leaf curl virus (TYLCV), a member of the family Geminiviridae, is a serious production constraint to tomato worldwide. In the new world, the virus had been identified as the causal agent of tomato yellow leaf curl disease in the Caribbean countries of the Dominican Republic, Cuba, and Guadeloupe and also in Florida and the Yucatan Peninsula (1). Molecular data from these TYLCV isolates identified the virus as the TYLCV prototype from Israel. Read More

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http://dx.doi.org/10.1094/PDIS-91-6-0768ADOI Listing

A Silicon-based Coral-like Nanostructured Microfluidics to Isolate Rare Cells in Human Circulation: Validation by SK-BR-3 Cancer Cell Line and Its Utility in Circulating Fetal Nucleated Red Blood Cells.

Micromachines (Basel) 2019 Feb 17;10(2). Epub 2019 Feb 17.

Department of Genomic Medicine and Center for Medical Genetics, Changhua Christian Hospital; and Department of Genomic Science and Technology, Changhua Christian Hospital Healthcare System, Changhua 50046, Taiwan.

Circulating fetal cells (CFCs) in maternal blood are rare but have a strong potential to be the target for noninvasive prenatal diagnosis (NIPD). "Cell Reveal system" is a silicon-based microfluidic platform capable to capture rare cell populations in human circulation. The platform is recently optimized to enhance the capture efficiency and system automation. Read More

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http://dx.doi.org/10.3390/mi10020132DOI Listing
February 2019

Roles of MicroRNA-34a in Epithelial to Mesenchymal Transition, Competing Endogenous RNA Sponging and Its Therapeutic Potential.

Int J Mol Sci 2019 Feb 16;20(4). Epub 2019 Feb 16.

Key Laboratory of Epigenetics and Oncology, the Research Center for Preclinical Medicine, Southwest Medical University, Luzhou 646000, China.

MicroRNA-34a (miR-34a), a tumor suppressor, has been reported to be dysregulated in various human cancers. MiR-34a is involves in certain epithelial-mesenchymal transition (EMT)-associated signal pathways to repress tumorigenesis, cancer progression, and metastasis. Due to the particularity of miR-34 family in tumor-associated EMT, the significance of miR-34a is being increasingly recognized. Read More

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http://dx.doi.org/10.3390/ijms20040861DOI Listing
February 2019

Influenza A virus (H1N1) triggers a hypoxic response by stabilizing hypoxia-inducible factor-1α via inhibition of proteasome.

Virology 2019 Feb 11;530:51-58. Epub 2019 Feb 11.

Department of Critical Care Medicine, Shanghai General Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 201620, China. Electronic address:

Virus reprogramming of host cellular function is a critical strategy for viral survival and replication. A better understanding of virus-host interaction may provide new potential avenues for the treatment of viral diseases. It has been reported that hypoxia-inducible factor-1 (HIF-1) pathway is activated by a range of pathogens via different mechanisms, but the impact of Influenza A virus on HIF-1 signaling is still unclear. Read More

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http://dx.doi.org/10.1016/j.virol.2019.02.010DOI Listing
February 2019

Blood-derived DNA Methylation Signatures of Crohn's Disease and Severity of Intestinal Inflammation.

Gastroenterology 2019 Feb 16. Epub 2019 Feb 16.

Genetics and Molecular Biology Program, Emory University, Atlanta, GA, USA; Division of Pediatric Gastroenterology, Department of Pediatrics, Emory University School of Medicine & Children's Healthcare of Atlanta, Atlanta, GA, USA; Department of Human Genetics, Emory University, Atlanta, GA, USA. Electronic address:

Background & Aims: Crohn's disease is a relapsing and remitting inflammatory disorder with a variable clinical course. Although most patients present with an inflammatory phenotype (B1), approximately 20% of patients rapidly progresses to complicated disease, which includes stricturing (B2) within 5 years. We analyzed DNA methylation patterns in blood samples of pediatric patients with Crohn's disease at diagnosis and later time points to identify changes that associate with and might contribute to disease development and progression. Read More

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http://dx.doi.org/10.1053/j.gastro.2019.01.270DOI Listing
February 2019

WDR72 Mutations Associated with Amelogenesis Imperfecta and Acidosis.

J Dent Res 2019 Feb 19:22034518824571. Epub 2019 Feb 19.

1 Department of Biologic and Materials Sciences, School of Dentistry, University of Michigan, Ann Arbor, MI, USA.

Dental enamel malformations, or amelogenesis imperfecta (AI), can be isolated or syndromic. To improve the prospects of making a successful diagnosis by genetic testing, it is important that the full range of genes and mutations that cause AI be determined. Defects in WDR72 (WD repeat-containing protein 72; OMIM *613214) cause AI, type IIA3 (OMIM #613211), which follows an autosomal recessive pattern of inheritance. Read More

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http://dx.doi.org/10.1177/0022034518824571DOI Listing
February 2019

Non-neuroendocrine neoplasms of the pituitary region.

J Clin Endocrinol Metab 2019 Feb 19. Epub 2019 Feb 19.

Centre for Endocrinology, Barts and the London School of Medicine, Queen Mary University of London, London, United Kingdom.

Context: Although the majority of sellar lesions are related to pituitary adenomas, the region gives rise to a variety of neoplasms that can be associated with substantial morbidity and/or mortality.

Evidence Acquisition: Information from reviews and guidelines of relevant societies dealing with such neoplasms, and articles that have provided new developments which made important contributions in their pathogenesis and treatment up to 2018, were obtained: public indexes such as PubMed/MEDLINE were used with the search items.

Evidence Synthesis: Sellar neoplasms have a worse outcome than pituitary adenomas that is related not only to their natural history but also to side effects of therapies and evolving endocrine and/or hypothalamic deficiencies. Read More

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http://dx.doi.org/10.1210/jc.2018-01871DOI Listing
February 2019

Parasite specific 7SL-derived small RNA is an effective target for diagnosis of active trypanosomiasis infection.

PLoS Negl Trop Dis 2019 Feb 19;13(2):e0007189. Epub 2019 Feb 19.

The Roslin Institute, Royal (Dick) School of Veterinary Studies, University of Edinburgh, Easter Bush, Midlothian, United Kingdom.

Human and animal African trypanosomiasis (HAT & AAT, respectively) remain a significant health and economic issue across much of sub-Saharan Africa. Effective control of AAT and potential eradication of HAT requires affordable, sensitive and specific diagnostic tests that can be used in the field. Small RNAs in the blood or serum are attractive disease biomarkers due to their stability, accessibility and available technologies for detection. Read More

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http://dx.plos.org/10.1371/journal.pntd.0007189
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http://dx.doi.org/10.1371/journal.pntd.0007189DOI Listing
February 2019
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High-Grade Sinonasal Carcinoma: Classification Through Molecular Profiling.

Arch Pathol Lab Med 2019 Feb 19. Epub 2019 Feb 19.

From the Department of Pathology, Massachusetts General Hospital and Harvard Medical School, Boston.

High-grade sinonasal carcinomas are a cohort of malignant epithelial neoplasms arising in the sinonasal cavities with distinct, ominous morphologic features or lacking well-differentiated features that might otherwise classify them as less biologically worrisome. Recent advances in molecular profiling have led to the identification of several distinct tumor entities previously grouped together. These molecularly distinct lesions include NUT (midline) carcinoma, INI1 ( SMARCB1)-deficient carcinoma, SMARCA4-deficient sinonasal carcinoma, and novel IDH-mutant sinonasal undifferentiated carcinoma, in addition to the previously described lymphoepithelial carcinoma that may also be included in the differential diagnosis. Read More

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http://dx.doi.org/10.5858/arpa.2018-0224-RSDOI Listing
February 2019

Identification of DNA mutations in gastric washes from gastric adenocarcinoma patients: possible implications for liquid biopsies and patient follow-up.

Int J Cancer 2019 Feb 18. Epub 2019 Feb 18.

Laboratory of Medical Genomics, A.C.Camargo Cancer Center, São Paulo, SP, Brazil.

Whereas cancer patients have benefited from liquid biopsies, the scenario for gastric adenocarcinoma (GAC) is still dismal. We used next-generation deep sequencing of TP53 - a highly mutated and informative gene in GAC - to assess mutations in tumor biopsies, plasma (PL) and stomach fluids (gastric wash - GW). We evaluated their potential to reveal tumor-derived mutations, useful for monitoring mutational dynamics at diagnosis, progression, and treatment. Read More

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http://dx.doi.org/10.1002/ijc.32217DOI Listing
February 2019