2,841,898 results match your criteria Molecular Cell [Journal]


Phenolic Profiles, Antioxidant and Antimicrobial Activities of Pawpaw Pulp (Asimina triloba [L.] Dunal) at Different Ripening Stages.

J Food Sci 2018 Dec 17. Epub 2018 Dec 17.

Dept. of Microbiology and Molecular Biology, Chungnam Natl. Univ., 99 Daehak-ro, Yuseong-gu, Daejeon, 34134, Republic of Korea.

In this study, the phenolic components, as well as the antioxidant and antimicrobial activities, of the ripe and unripe fruit of pawpaw (Asimina triloba [L.] Dunal) extracted using five different solvents (distilled water, 95% methanol, 80% methanol, 95% ethanol, and 80% ethanol) were analyzed. The total phenolic content and total flavonoid content were the highest in the 95% ethanol (149. Read More

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December 2018

Platelet-Dense Granules Worsen Pre-Infection Thrombocytopenia during Gram-Negative Pneumonia-Derived Sepsis.

J Innate Immun 2018 Dec 17:1-13. Epub 2018 Dec 17.

Center for Experimental and Molecular Medicine, Academic Medical Center, University of Amsterdam, Amsterdam, The Netherlands.

Platelet-dense (δ) granules are important for platelet function. Platelets contribute to host defense and vascular integrity during pneumonia and sepsis, and δ granule products, including adenosine diphosphate (ADP), can influence inflammatory responses. We therefore aimed to study the role of platelet δ granules in the host response during sepsis. Read More

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December 2018

Phenotypic Heterogeneity within Members of a Family Carrying the Same RBM20 Mutation R634W.

Cardiology 2018 Dec 17;141(3):150-155. Epub 2018 Dec 17.

Molecular Immunopathology and Histocompatibility Unit, Division of Molecular Genetics, Onassis Cardiac Surgery Center, Athens, Greece.

Objective: We present the genotypic and phenotypic characterization of a family displaying dilated cardiomyopathy (DCM).

Methods: The proband and his relatives underwent full cardiological assessment. Genetic analysis of the proband was performed with the use of next-generation sequencing technology. Read More

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December 2018

Towards Understanding the Molecular Basis of Nitric Oxide-Regulated Group Behaviors in Pathogenic Bacteria.

J Innate Immun 2018 Dec 17:1-11. Epub 2018 Dec 17.

Pathogenic bacteria have many strategies for causing disease in humans. One such strategy is the ability to live both as single-celled motile organisms or as part of a community of bacteria called a biofilm. Biofilms are frequently adhered to biotic or abiotic surfaces and are extremely antibiotic resistant. Read More

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December 2018

Downregulation of Microparticle Release and Pro-Inflammatory Properties of Activated Human Polymorphonuclear Neutrophils by LMW Fucoidan.

J Innate Immun 2018 Dec 17:1-17. Epub 2018 Dec 17.

Laboratório de Farmacologia Celular e Molecular, Universidade do Estado do Rio de Janeiro, Rio de Janeiro,

Exposition of neutrophils (polymorphonuclear neutrophils, PMNs) to bacterial products triggers exacerbated activation of these cells, increasing their harmful effects on host tissues. We evaluated the possibility of interfering with the classic immune innate responses of human PMNs exposed to bacterial endotoxin (lipopolysaccharide, LPS), and further stimulated with bacterial formyl peptide (N-formyl-methionine-leucine-phenylalanine, fMLP). We showed that the low- molecular-weight fucoidan (LMW-Fuc), a polysaccharide extracted from brown algae, attenuated the exacerbated activation induced by fMLP on LPS-primed PMNs, in vitro, impairing chemotaxis, NET formation, and the pro-survival and pro-oxidative effects. Read More

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December 2018

O pollution in a future climate increases the competition between summer rape and wild mustard.

Plant Physiol Biochem 2018 Dec 11;135:194-205. Epub 2018 Dec 11.

Vytautas Magnus University, Department of Environmental Sciences, Vileikos St. 8-223, LT-44404, Kaunas, Lithuania.

The initial aim of this study was to evaluate an effect of elevated CO concentration and air temperature (future climate) and O pollution on mono- and mixed-culture grown summer rape (Brassica napus L.) and wild mustard (Sinapis arvensis L.). Read More

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December 2018

Achieving highly sensitive detection of Cu based on AIE and FRET strategy in aqueous solution.

Spectrochim Acta A Mol Biomol Spectrosc 2018 Dec 11;211:272-279. Epub 2018 Dec 11.

Key Laboratory of Chemical Biology, Molecular Engineering of Ministry of Education, Institute of Molecular Science, Shanxi University, Taiyuan 030006, China. Electronic address:

The aggregation-induced emission (AIE) luminogens are now showing strong potential in mimicking the energy donor of fluorescence resonance energy transfer (FRET) system. Herein, one highly efficient FRET system 1-NiR is successfully fabricated in aqueous solution based on an AIE active compound 1 and fluorescence dyes (Nile red (NiR)). 1 acts as the energy donor and NiR acts as the acceptor in the FRET system with the optimum concentrations ratio [1]/[NiR] = 100. Read More

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December 2018

Regulation of inositol 1,4,5-trisphosphate-induced Ca release from the endoplasmic reticulum by AMP-activated kinase modulators.

Cell Calcium 2018 Dec 11;77:68-76. Epub 2018 Dec 11.

Institute of Biology and Molecular Genetics (IBGM), Department of Biochemistry and Molecular Biology and Physiology, Faculty of Medicine, University of Valladolid and CSIC, Ramón y Cajal, 7, E-47005 Valladolid, Spain. Electronic address:

The 5' AMP-activated protein kinase (AMPK) is a nutrient-sensitive kinase that plays a key role in the control of cellular energy metabolism. We have explored here the relationship between AMPK and Ca signaling by looking at the effect of an AMPK activator (A769662) and an AMPK inhibitor (dorsomorphin) on histamine-induced Ca-release from the endoplasmic reticulum (ER) in HeLa cells. Our data show that incubation with A769662 (EC = 29 μM) inhibited histamine-induced Ca-release from the ER in intact cells, as well as inositol-1,4,5-trisphosphate (IP)-induced Ca release in permeabilized cells. Read More

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December 2018

Sandwich-type electrochemical immunosensor for sensitive detection of CEA based on the enhanced effects of Ag NPs@CS spaced Hemin/rGO.

Biosens Bioelectron 2018 Dec 7;126:785-791. Epub 2018 Dec 7.

School of Chemical Engineering, Shandong University of Technology, Zibo 255049, PR China.

An ultrasensitive sandwich-type electrochemical immunosensor was designed by using gold nanoparticles (Au NPs) as the substrate material and microporous carbon spheres (CS) loading silver nanoparticles (Ag NPs) spaced Hemin/reduced graphene oxide (Hemin/rGO) porous composite materials (Ag NPs@CS-Hemin/rGO) as the detection antibodies (Ab) label for detecting carcinoembryonic antigen (CEA). The Au NPs with good electrical conductivity and biocompatibility could accelerate the electron transfer on the electrode interface and enhance the load capacity of capture antibodies (Ab). Hemin is peroxidase-like substance which has excellent catalytic ability for HO reduction but easy to molecular aggregation and oxidative self-destruction. Read More

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December 2018

Current antifungal drugs and immunotherapeutic approaches as promising strategies to treatment of fungal diseases.

Biomed Pharmacother 2018 Dec 14;110:857-868. Epub 2018 Dec 14.

Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran; Department of Immunology, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran. Electronic address:

Invasive candidiasis (IC) can affect individuals with various underlying diseases hospitalized in different parts of hospitals. In recent decades, IC has caused 27-55% mortality in general population. Although Candida albicans (C. Read More

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December 2018

Treating hyperuricemia related non-alcoholic fatty liver disease in rats with resveratrol.

Biomed Pharmacother 2018 Dec 14;110:844-849. Epub 2018 Dec 14.

The Hangzhou Xixi Hospital Affiliated to Zhejiang Chinese Medical University, Hangzhou 310023, Zhejiang, China. Electronic address:

Background Hyperuricemia is a recognised risk factor for the development of nonalcoholic fatty liver disease (NAFLD). This study aims to investigate the therapeutic effect of resveratrol (RES) on the treatment of hyperuricemia-related NAFLD in rats and the underlying mechanisms. Methods NAFLD with hyperuricemia was induced in rats using high-yeast high-fat diet containing potassium oxonate. Read More

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December 2018

Unravel a neuroactive sHA sulfation pattern with neurogenesis activity by a library of defined oligosaccharides.

Eur J Med Chem 2018 Dec 4;163:583-596. Epub 2018 Dec 4.

State Key Laboratory of Natural and Biomimetic Drugs, Department of Chemical Biology, School of Pharmaceutical Sciences, Peking University, No. 38 Xueyuan Road, 100191, Haidian District, Beijing, China. Electronic address:

Sulfated hyaluronic acid (sHA) is chemically synthetic mimetic of glycosaminoglycan (GAG) presenting promising biological functions. Specific sulfation pattern, termed as sulfation code plays critical roles in regulating the binding mode between GAG and proteins. As a structural analogue of chondroitin sulfate (CS), sHA bears much higher molecular weight and is nearly free of other proteoglycan contaminants. Read More

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December 2018

Surface enhanced Raman scattering with methyl-orange on Ag-TiO nanocomposites: A computational investigation.

J Mol Graph Model 2018 Nov 26;87:220-226. Epub 2018 Nov 26.

Department of Physics, University of the Free State, Bloemfontein, 9300, South Africa; Department of Chemistry, National Institute of Technology (NIT) Hamirpur, Himachal Pradesh 177005, India.

The influence of changing Ag concentration in a Ag-TiO nanocomposite system was investigated with molecular dynamics (MD) simulations for both a fixed as well as varying number of the surfactant MO adsorbed onto the nanocomposite. Density functional theory (DFT) simulations were performed to investigate the conditions for charge transfer from the MO surfactant via the highest occupied molecular orbitals (HOMO), lowest unoccupied molecular orbitals (LUMO) as well as the electrostatic potentials. It is observed in this study that the bonding mode of the surfactant contributes greatly to the observed Raman scattering enhancement. Read More

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November 2018

Identification of novel PI3Kδ inhibitors by docking, ADMET prediction and molecular dynamics simulations.

Comput Biol Chem 2018 Dec 7;78:190-204. Epub 2018 Dec 7.

Tianjin Key Laboratory on Technologies Enabling Development of Clinical Therapeutics and Diagnostics (Theranostics), School of Pharmacy, Tianjin Medical University, Tianjin 300070, China. Electronic address:

Background: Phosphoinositide-3-kinase Delta (PI3Kδ) plays a key role in B-cell signal transduction and inhibition of PI3Kδ is confirmed to have clinical benefit in certain types of activation of B-cell malignancies. Virtual screening techniques have been used to discover new molecules for developing novel PI3Kδ inhibitors with little side effects.

Method: Computer aided drug design method were used to rapidly screen optimal PI3Kδ inhibitors from the Asinex database. Read More

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December 2018

Identification of novel PPARα/γ dual agonists by pharmacophore screening, docking analysis, ADMET prediction and molecular dynamics simulations.

Comput Biol Chem 2018 Nov 27;78:178-189. Epub 2018 Nov 27.

Tianjin Key Laboratory on Technologies Enabling Development of Clinical Therapeutics and Diagnostics (Theranostics), School of Pharmacy, Tianjin Medical University, Tianjin, China. Electronic address:

PPARα and PPARγ play an important role in regulating glucose and lipid metabolism. The single and selective PPARα or PPARγ agonists have caused several side effects such as edema, weight gain and cardiac failure. In the recent years, the dual PPARs agonist development has become a hot topic in the antidiabetic medicinal chemistry field. Read More

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November 2018

Association between exposure to a mixture of phenols, pesticides, and phthalates and obesity: Comparison of three statistical models.

Environ Int 2018 Dec 14;123:325-336. Epub 2018 Dec 14.

State Key Laboratory of Reproductive Medicine, Center for Global Health, School of Public Health, Nanjing Medical University, Nanjing, 211166, China; Key Laboratory of Modern Toxicology of Ministry of Education, School of Public Health, Nanjing Medical University, Nanjing 211166, China. Electronic address:

Background: The evaluation of the chemical impact on human health is usually constrained to the analysis of the health effects of exposure to a single chemical or a group of similar chemicals at one time. The effects of chemical mixtures are seldom analyzed. In this study, we applied three statistical models to assess the association between the exposure to a mixture of seven xenobiotics (three phthalate metabolites, two phenols, and two pesticides) and obesity. Read More

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December 2018

Fine particle-bound polycyclic aromatic hydrocarbons (PAHs) at an urban site of Wuhan, central China: Characteristics, potential sources and cancer risks apportionment.

Environ Pollut 2018 Dec 3;246:319-327. Epub 2018 Dec 3.

State Key Laboratory of Biogeology and Environmental Geology, China University of Geosciences, Wuhan, 430074, China; Department of Environmental Science and Engineering, School of Environmental Studies, China University of Geosciences, Wuhan, 430074, China.

Levels, compositions, sources, and cancer risks of fine particle (PM)-bound PAHs were investigated at an urban site of Wuhan, Central China. Totally 115 PM samples collected during four seasons from 2014 to 2015 were analyzed for 16 USEPA priority PAHs. The annual average of PM and total PAHs were 106 ± 41. Read More

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December 2018

Assessment of forest fire impacts on carbonaceous aerosols using complementary molecular marker receptor models at two urban locations in California's San Joaquin Valley.

Environ Pollut 2018 Dec 7;246:274-283. Epub 2018 Dec 7.

Department of Civil & Environmental Engineering, University of Wisconsin-Madison, Madison, 53705, USA; Environmental Chemistry and Technology, University of Wisconsin-Madison, Madison, 53706, USA. Electronic address:

Two hundred sixty-three fine particulate matter (PM) samples were collected over fourteen months in Fresno and Bakersfield, California. Samples were analyzed for organic carbon (OC), elemental carbon (EC), water soluble organic carbon (WSOC), and 160 organic molecular markers. Chemical Mass Balance (CMB) and Positive Matrix Factorization (PMF) source apportionment models were applied to the results in order to understand monthly and seasonal source contributions to PM OC. Read More

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December 2018

Biosynthesized of reduced graphene oxide nanosheets and its loading with paclitaxel for their anti cancer effect for treatment of lung cancer.

J Photochem Photobiol B 2018 Nov 22;191:13-17. Epub 2018 Nov 22.

Department of pharmacy, Fujian Provincial Hospital& Fujian Medical University, People's Republic of China; Molecular Biology Laboratory of Chinese Traditional Medicine, Fujian Provincial Hospital, People's Republic of China. Electronic address:

Multifunctional nanocarriers are in immediate need to develop anticancer activity for the treatment of cancers. In the present research, the graphene oxide was reduced via an efficient method which reduced to RGO by using Euphorbia milii leaves extract. Thus obtained RGO nanocomposites were subsequently characterized by means UV-Vis absorption technique. Read More

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November 2018

Neuronal Receptors Display Cytoskeleton-Independent Directed Motion on the Plasma Membrane.

iScience 2018 Dec 5;10:234-244. Epub 2018 Dec 5.

Centre for Developmental Neurobiology, Institute of Psychiatry, Psychology and Neuroscience, King's College London, London SE1 1UL, UK; MRC Centre for Neurodevelopmental Disorders, King's College London, New Hunt's House, Guy's Campus, London SE1 1UL, UK. Electronic address:

Directed transport of transmembrane proteins is generally believed to occur via intracellular transport vesicles. However, using single-particle tracking in rat hippocampal neurons with a pH-sensitive quantum dot probe that specifically reports surface movement of receptors, we have identified a subpopulation of neuronal EphB2 receptors that exhibit directed motion between synapses within the plasma membrane itself. This receptor movement occurs independently of the cytoskeleton but is dependent on cholesterol and is regulated by neuronal activity. Read More

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December 2018
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Mapping whole brain connectivity changes: The potential impact of different surgical resection approaches for temporal lobe epilepsy.

Cortex 2018 Nov 17;113:1-14. Epub 2018 Nov 17.

Neuroscience and Aphasia Research Unit, Division of Neuroscience and Experimental Psychology, School of Biological Sciences, University of Manchester, UK; MRC Cognition and Brain Sciences Unit, University of Cambridge, UK. Electronic address:

In neurosurgery there are several situations that require transgression of the temporal cortex. For example, a subset of patients with temporal lobe epilepsy require surgical resection (most typically, en-bloc anterior temporal lobectomy). This procedure is the gold standard to alleviate seizures but is associated with chronic cognitive deficits. Read More

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November 2018
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Oxidative damage induced by copper in testis of the red swamp crayfish Procambarus clarkii and its underlying mechanisms.

Aquat Toxicol 2018 Dec 6;207:120-131. Epub 2018 Dec 6.

Ecological Society of Shandong, Zhijinshi Jie 12, Jinan 250012, China; Shandong Institute of Environmental Science, Lishan Lu 50, Jinan 250013, China. Electronic address:

Copper (Cu) is one of the most widespread environmental pollutants and is known to exert multiple toxic effects including reproductive toxicity. In this study, we investigated the toxic effect of Cu on reproduction of the red swamp crayfish (Procambarus clarkii), an economic crustacean species, by exposing adult male crayfish to 0.03 and 3. Read More

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December 2018

Cobalt tetracarboxyl phthalocyanine-manganese octahedral molecular sieve (OMS-2) as a heterogeneous catalyst of peroxymonosulfate for degradation of diclofenac.

Chemosphere 2018 Dec 5;219:756-765. Epub 2018 Dec 5.

Shanghai Institute of Pollution Control and Ecological Security, Key Laboratory of Yangtze River Water Environment Ministry of Education, College of Environmental Science and Engineering, Tongji University, Shanghai 200092, China. Electronic address:

A composite of cobalt tetracarboxyl phthalocyanine and amino-functionalized manganese octahedral molecular sieve (CNOMS-2) was synthesized in a simple way, and applied for degrading diclofenac in aqueous media by catalyzing of peroxymonosulfate heterogeneously. The experiment results revealed that the CNOMS-2/PMS system was highly efficient for DCF degradation in the pH range of 5-9, and the mechanism involved the generation of OH, SO⁻ and O by the activation of PMS. The main reactive oxygen species was found to be O by radical scavenging experiments and electron paramagnetic resonance (EPR) spectroscopy. Read More

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December 2018

Immediate Hypersensitivity to Polyethylene Glycols and Polysorbates: More Common Than We Have Recognized.

J Allergy Clin Immunol Pract 2018 Dec 14. Epub 2018 Dec 14.

Division of Infectious Diseases, Department of Medicine, Vanderbilt University Medical Center, Nashville, Tennessee, USA.

Background: The most common immediate hypersensitivity to macrogols is associated with PEG 3350, however the epidemiology, mechanisms and cross-reactivity are poorly understood. Thousands of medications contain either PEGs or structurally similar polysorbates.

Objective: Our objective was to better understand the mechanism, cross-reactivity and scope of PEG hypersensitivity. Read More

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December 2018
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Metabolic perturbation, proliferation and reactive oxygen species jointly contribute to cytotoxicity of human breast cancer cell induced by tetrabromo and tetrachloro bisphenol A.

Ecotoxicol Environ Saf 2018 Dec 14;170:495-501. Epub 2018 Dec 14.

State Key Laboratory of Environmental and Biological Analysis, Department of Chemistry, Hong Kong Baptist University, Hong Kong, China. Electronic address:

Halogenated bisphenol A analogues (X-BPA) have been widely used in industrial production, such as flame retardant. Although BPA exposure was found to result in cytotoxicity, toxicity of X-BPA and molecular mechanism remain under-explored. In this study, we employed human breast cancer cell as a model to investigate the concentration-dependent toxicity and underlying mechanisms of tetrabromo bisphenol A (TBBPA) and tetrachloro bisphenol A (TCBPA). Read More

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December 2018

Profiling of miRNAs in serum of children with attention-deficit hyperactivity disorder shows significant alterations.

J Psychiatr Res 2018 Dec 8;109:185-192. Epub 2018 Dec 8.

Cellular and Molecular Research Center, Yasuj University of Medical Sciences, Yasuj, Iran; CEINGE-biotecnologie Avanzate, Naples, Italy. Electronic address:

Background: Attention-deficit/hyperactivity disorder (ADHD), a common psychiatric disorder, is identified by abnormal levels of impulsivity, inattention, and hyperactivity. MiRNAs play important roles in neural network development of the brain. Circulating miRNAs (cmiRNAs) are offered as promising noninvasive markers for psychiatric disorders. Read More

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December 2018

The Intestinal Lymphatic System: Functions and Metabolic Implications.

Cell Mol Gastroenterol Hepatol 2018 Dec 14. Epub 2018 Dec 14.

Cardiovascular Research Center and Department of Cellular and Molecular Physiology, Yale University School of Medicine, New Haven, CT 06510-3221, USA; INSERM U970, Paris Cardiovascular Research Center, 75015 Paris, France.

The lymphatic system of the gut plays important roles in the transport of dietary lipids, as well as in immunosurveillance and removal of interstitial fluid. Historically, despite its crucial functions in intestinal homeostasis, the lymphatic system has been poorly studied. In the last two decades, identification of specific molecular mediators of lymphatic endothelial cells (LECs) growth together with novel genetic approaches and intravital imaging techniques, have advanced our understanding of the mechanisms regulating intestinal lymphatic physiology in health and disease. Read More

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December 2018
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Ligation of CD180 contributes to endotoxic shock by regulating the accumulation and immunosuppressive activity of myeloid-derived suppressor cells through STAT3.

Biochim Biophys Acta Mol Basis Dis 2018 Dec 14. Epub 2018 Dec 14.

Department of Medicine, Immunology Institute, Icahn School of Medicine at Mount Sinai, New York, NY 10029, USA. Electronic address:

Myeloid-derived suppressor cells (MDSCs) play an immunosuppressive role in the pathogenesis of inflammatory diseases. CD180, a TLR-like protein, can regulate the proliferation and activation of immune cells. However, the roles of CD180 in regulating the accumulation and function of MDSCs have not been investigated. Read More

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December 2018

A mouse model for intellectual disability caused by mutations in the X-linked 2'‑O‑methyltransferase Ftsj1 gene.

Biochim Biophys Acta Mol Basis Dis 2018 Dec 14. Epub 2018 Dec 14.

Department of Functional Genomics, Interfaculty Institute of Genetics and Functional Genomics, University Medicine Greifswald, Felix-Hausdorff-Strasse 8, 17489 Greifswald, Germany.

Mutations in the X chromosomal tRNA 2'‑O‑methyltransferase FTSJ1 cause intellectual disability (ID). Although the gene is ubiquitously expressed affected individuals present no consistent clinical features beyond ID. In order to study the pathological mechanism involved in the aetiology of FTSJ1 deficiency-related cognitive impairment, we generated and characterized an Ftsj1 deficient mouse line based on the gene trapped stem cell line RRD143. Read More

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December 2018

In vitro Drug Release from Acetylated High Amylose Starch-Zein Films for Oral Colon-Specific Drug Delivery.

Int J Pharm 2018 Dec 14. Epub 2018 Dec 14.

School of Pharmacy, University of East Anglia, Norwich NR7 1AT, UK. Electronic address:

This study describes the preparation of free films of zein with and without acetylated high amylose maize starch (HAS) and their corresponding coated tablets as a novel approach to colonic drug delivery. We hypothesise that the embedding of a digestible starch component within the inert zein would allow the film to remain intact until the large intestine is reached. Free films of zein alone and starch/zein were prepared and characterised. Read More

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December 2018

Understanding the transition from water to land: Insights from multi-omic analyses of the perivitelline fluid of apple snail eggs.

J Proteomics 2018 Dec 14. Epub 2018 Dec 14.

Department of Biology, Hong Kong Baptist University, Hong Kong, China; HKBU Institute of Research and Continuing Education, Shenzhen, China. Electronic address:

Most gastropods deposit their eggs either on land or underwater, but the family Ampullariidae includes members that exhibit both underwater and aerial oviposition, making it an ideal model for understanding mechanisms underlying the evolutionary transition from water to land. We applied SDS-PAGE and LC-MS/MS to analyse the proteome of the egg perivitelline fluid (PVF) of Marisa cornuarietis - an aquatic ovipositing ampullariid. Comparison with the reported PVF proteomes of two aerial ovipositing ampullariids (Pomacea canaliculata and P. Read More

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December 2018
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Comprehensive identification of protein disulfide bonds with pepsin/trypsin digestion, Orbitrap HCD and Spectrum Identification Machine.

J Proteomics 2018 Dec 14. Epub 2018 Dec 14.

Center for Advanced Proteomics Research and Department of Microbiology, Biochemistry and Molecular Genetics, Rutgers University - New Jersey Medical School and Cancer Institute of New Jersey, Newark, NJ 07103, USA. Electronic address:

Disulfide bonds (SS) are post-translational modifications important for the proper folding and stabilization of many cellular proteins with therapeutic uses, including antibodies and other biologics. With budding advances of biologics and biosimilars, there is a mounting need for a robust method for accurate identification of SS. Even though several mass spectrometry methods have emerged for this task, their practical use rests on the broad effectiveness of both sample preparation methods and bioinformatics tools. Read More

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December 2018

Dissecting the molecular effects of cigarette smoke on proteasome function.

J Proteomics 2018 Dec 14. Epub 2018 Dec 14.

Comprehensive Pneumology Center (CPC), University Hospital, Ludwig-Maximilians University, Helmholtz Zentrum München, Member of the German Center for Lung Research (DZL), Munich, Germany. Electronic address:

Proteasome dysfunction is emerging as a novel pathomechanism for the development of chronic obstructive pulmonary disease (COPD), a major leading cause of death in the world. Cigarette smoke, one of the main risk factors for COPD, impairs proteasome function in vitro and in vivo. In the present study, we dissected the molecular changes induced by cigarette smoke on the proteasome in lung epithelial cells and mouse lungs. Read More

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December 2018

Non-invasive MRI of brain clearance pathways using multiple echo time arterial spin labelling: An Aquaporin-4 study.

Neuroimage 2018 Dec 14. Epub 2018 Dec 14.

UCL Centre for Advanced Biomedical Imaging, Division of Medicine, UCL, London, UK.

There is currently a lack of non-invasive tools to assess water transport in healthy and pathological brain tissue. Aquaporin-4 (AQP4) water channels are central to many water transport mechanisms, and emerging evidence also suggests that AQP4 plays a key role in amyloid-β (Aβ) clearance, possibly via the glymphatic system. Here, we present the first non-invasive technique sensitive to AQP4 channels polarised at the blood-brain interface (BBI). Read More

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December 2018

A multiomic approach to characterize the temporal sequence in Alzheimer's disease-related pathology.

Neurobiol Dis 2018 Dec 14. Epub 2018 Dec 14.

Institute of Biomedicine, University of Eastern Finland, Kuopio 70210, Finland. Electronic address:

No single-omic approach completely elucidates the multitude of alterations taking place in Alzheimer's disease (AD). Here, we coupled transcriptomic and phosphoproteomic approaches to determine the temporal sequence of changes in mRNA, protein, and phosphopeptide expression levels from human temporal cortical samples, with varying degree of AD-related pathology. This approach highlighted fluctuation in synaptic and mitochondrial function as the earliest pathological events in brain samples with AD-related pathology. Read More

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December 2018

Phycoerythrin-phycocyanin aggregates and phycoerythrin aggregates from phycobilisomes of the marine red alga Polysiphonia urceolata.

Int J Biol Macromol 2018 Dec 14. Epub 2018 Dec 14.

College of Life Sciences, Yantai University, Shandong 264005, PR China.

Phycoerythrin-phycocyanin aggregates and phycoerythrin aggregates showing special spectral characteristics were prepared from the partly dissociated products of phycobilisomes from Polysiphonia urceolata. The absorption difference spectra between the aggregates and phycoerythrins showing normal spectral characteristics show peaks at 583 nm. The fluorescence emission difference spectra between the phycoerythrin aggregates and normal phycoerythrins show peaks at 602 nm. Read More

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December 2018

Gene cloning, expression, molecular modeling and docking study of the protease SAPRH from Bacillus safensis strain RH12.

Int J Biol Macromol 2018 Dec 14. Epub 2018 Dec 14.

Laboratory of Microbial Biotechnology and Engineering Enzymes (LMBEE), Centre of Biotechnology of Sfax (CBS), University of Sfax, Road of Sidi Mansour, Km 6, P.O. Box 1177, Sfax 3018, Tunisia; Biotech ECOZYM Start-up, Business Incubator, Centre of Biotechnology of Sfax (CBS), University of Sfax, Road of Sidi Mansour Km 6, P.O. Box 1177, Sfax 3018, Tunisia. Electronic address:

The sapRH gene, which encodes the serine alkaline protease SAPRH, from Bacillus safensis RH12, was isolated and its DNA sequence was determined. The deduced amino-acid sequence showed strong homology with other Bacillus proteases. The highest sequence identity value (97%) was obtained with SAPB from B. Read More

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December 2018

Role of anoctamin 5, a gene associated with gnathodiaphyseal dysplasia, in osteoblast and osteoclast differentiation.

Bone 2018 Dec 14. Epub 2018 Dec 14.

Department of Pharmacology, Chonnam National University Medical School, Gwangju 61469, Republic of Korea; Department of Biomedical Sciences, Chonnam National University Medical School, Gwangju 61469, Republic of Korea. Electronic address:

Anoctamin 5 (Ano5) mutations are responsible for gnathodiaphyseal dysplasia, a rare skeletal syndrome. Despite the close linkage of Ano5 to bone remodeling, the molecular mechanisms underlying the role of Ano5 in bone remodeling remain unknown. In this study, we investigated whether Ano5 regulates osteoblast or osteoclast differentiation to maintain normal bone remodeling. Read More

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December 2018

KIT as a therapeutic target for non-oncological diseases.

Pharmacol Ther 2018 Dec 14. Epub 2018 Dec 14.

Aix Marseille Université, INSERM, INRA, C2VN, Marseille, France; Clinique des Bronches, Allergies et Sommeil, Hôpital Nord, AP-, HM, Marseille, France.. Electronic address:

KIT is a receptor tyrosine kinase that after binding to its ligand stem cell factor activates signaling cascades linked to biological processes such as proliferation, differentiation, migration and cell survival. Based on studies performed on SCF and/or KIT mutant animals that presented anemia, sterility, and/or pigmentation disorders, KIT signaling was mainly considered to be involved in the regulation of hematopoiesis, gametogenesis, and melanogenesis. More recently, novel animal models and ameliorated cellular and molecular techniques have led to the discovery of a widen repertoire of tissue compartments and functions that are being modulated by KIT. Read More

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December 2018
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Mechanisms of cardiac ethanol toxicity and novel treatment options.

Pharmacol Ther 2018 Dec 14. Epub 2018 Dec 14.

Department of Internal Medicine II, University Medical Center Regensburg, Germany. Electronic address:

Ethanol can acutely and chronically alter cardiomyocyte and whole-organ function in the heart. Importantly, ethanol acutely and chronically predisposes to arrhythmias, while chronic abuse can induce heart failure. However, the molecular mechanisms of ethanol toxicity in the heart are incompletely understood. Read More

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December 2018

Single-molecule 3D imaging of human plasma intermediate-density lipoproteins reveals a polyhedral structure.

Biochim Biophys Acta Mol Cell Biol Lipids 2018 Dec 14. Epub 2018 Dec 14.

The Molecular Foundry, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA. Electronic address:

Intermediate-density lipoproteins (IDLs), the remnants of very-low-density lipoproteins via lipolysis, are rich in cholesteryl ester and are correlated with cardiovascular disease. Despite pharmacological interests in IDL, the three-dimensional (3D) structure of IDLs is still undetermined due to their variation in size, compositions, and dynamic structure. To explore the 3D structure of IDL, we reconstructed 3D density maps from each individual IDL particles using cryo-electron microscopy (cryo-EM) and individual-particle electron tomography (IPET, without averaging from different molecules). Read More

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December 2018

Induced Pluripotent Stem Cells: A New Strategy to Model Human Cancer.

Int J Biochem Cell Biol 2018 Dec 14. Epub 2018 Dec 14.

Laboratory for Cancer Biology, Department of Medical Oncology and Clinical Research, Cancer Institute (WIA), Chennai, India. Electronic address:

Induced pluripotent stem cells are derived from adult somatic cells by ectopic expression of stem cell factors OCT4, SOX2, MYC and KLF4. These cells have characteristic features similar to embryonic stem cells. Although there exists in vitro and in vivo models of cancer, recapitulating the earliest events in the pathogenesis remain challenging. Read More

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December 2018

DHA inhibits proliferation and induces ferroptosis of leukemia cells through autophagy dependent degradation of ferritin.

Free Radic Biol Med 2018 Dec 14. Epub 2018 Dec 14.

Department of Laboratory Medicine, Zhejiang Provincial People's Hospital, People's Hospital of Hangzhou Medical College, Hangzhou, Zhejiang 310014, China; Clinical Research Institute, Zhejiang Provincial People's Hospital, People's Hospital of Hangzhou Medical College, Hangzhou, Zhejiang 310014, China. Electronic address:

Dihydroartemisinin (DHA) has been shown to be capable of inhibiting cancer growth, whereas it remains largely elusive that the underlying molecular mechanism of DHA induced acute myeloid leukemia (AML) cell death. In the present study, we examined the effects of DHA on the proliferation and ferroptosis of AML cells as well as to elucidate the underlying molecular mechanisms. We found that DHA strongly inhibited the viability of AML cell lines and arrest cell cycle at G0/G1 phase. Read More

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December 2018

Aquatic animal viruses mediated immune evasion in their host.

Authors:
Fei Ke Qi-Ya Zhang

Fish Shellfish Immunol 2018 Dec 14. Epub 2018 Dec 14.

State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, 430072, China. Electronic address:

Viruses are important and lethal pathogens that hamper aquatic animals. The result of the battle between host and virus would determine the occurrence of diseases. The host will fight against virus infection with various responses such as innate immunity, adaptive immunity, apoptosis, and so on. Read More

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December 2018

Molecular cloning and expression analysis of coagulation factor VIII and plasminogen involved in immune response to GCRV, and immunity activity comparison of grass carp Ctenopharyngodon idella with different viral resistance.

Fish Shellfish Immunol 2018 Dec 14. Epub 2018 Dec 14.

Hunan Engineering Technology Research Center of Featured Aquatic Resources Utilization, Hunan Agricultural University, Changsha, 410128, China; Collaborative Innovation Center for Efficient and Health Production of Fisheries in Hunan Province, Changde, Hunan, 415000, China. Electronic address:

The grass carp reovirus (GCRV) has been shown to cause lethal infections in the grass carp Ctenopharyngodon idella (C. idella). In order to investigate the immune response to GCRV infection, the full-length cDNA sequences of coagulation factor VIII (CiFVIII) and plasminogen (CiPLG) from C. Read More

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December 2018
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TCF7L2 and EGR1 synergistic activation of transcription of LCN2 via an ERK1/2-dependent pathway in esophageal squamous cell carcinoma cells.

Cell Signal 2018 Dec 14. Epub 2018 Dec 14.

Department of Pathology, Shantou Central Hospital, Affiliated Shantou Hospital of Sun Yat-sen University, Shantou 515041, China; Guangdong Province Key Laboratory of Malignant Tumor Epigenetics and Genes Regulation, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou 510120, China. Electronic address:

High level expression of lipocalin 2 (LCN2) usually indicates poor prognosis in esophageal squamous cell carcinoma (ESCC) and many other cancers. Our previous study showed LCN2 promotes migration and invasion of ESCC cells through a novel positive feedback loop. However, the key transcription activation protein (KTAP) in the loop had not yet been identified. Read More

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December 2018

Dioclea violacea lectin ameliorates inflammation in the temporomandibular joint of rats by suppressing intercellular adhesion molecule-1 expression.

Biochimie 2018 Dec 14. Epub 2018 Dec 14.

Faculdade São Leopoldo Mandic, Instituto de Pesquisas São Leopoldo Mandic, Área de Imunologia, Campinas, Brazil. Electronic address:

Inflammation of temporomandibular joint (TMJ) tissues are the most common cause of pain conditions associated with temporomandibular disorders (TMDs). After a tissue and/or neural damage, the inflammatory response is characterized by plasma extravasation and leukocytes infiltration in the TMJ tissues, which in turn, release inflammatory cytokines cascades responsible for inflammatory pain. Lectins are glycoproteins widely distributed in nature that may exhibit anti-inflammatory properties. Read More

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December 2018

Expression of immediate early genes in brain reward circuitries: Differential regulation by psychostimulant and opioid drugs.

Neurochem Int 2018 Dec 14. Epub 2018 Dec 14.

NIDA Intramural Program, Molecular Neuropsychiatry Research Branch, 251 Bayview Boulevard, Baltimore, MD, 21224, USA. Electronic address:

Although some of the clinical manifestations of substance use disorders might be superficially similar, it is highly likely that different classes of abused drugs including opioids (heroin, morphine, and oxycodone, other opioids) and psychostimulants (cocaine and amphetamines) cause different neuroadaptations in various brain regions dependent in the distribution and concentration of their biochemical sites of actions. In fact, different molecular networks are indeed impacted by acute and chronic administration of addictive substances. Some of the genes whose expression is influenced by the administration of these substances are immediate-early genes (IEGs). Read More

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December 2018

Nicotine protects against manganese and iron-induced toxicity in SH-SY5Y cells: Implication for Parkinson's disease.

Neurochem Int 2018 Dec 14. Epub 2018 Dec 14.

Department of Pharmacology, Howard University College of Medicine, Washington, DC, USA. Electronic address:

Manganese (Mn) and iron (Fe) are trace elements that are essential for proper growth and physiological functions as both play critical role in a variety of enzymatic reactions. At high concentrations, however, they can be toxic and cause neurodegenerative disorders, particularly Parkinson-like syndromes. Nicotine, on the other hand, has been shown to have neuroprotective effects against various endogenous or exogenous toxins that selectively damage the dopaminergic cells. Read More

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December 2018

Molecular characterization of newly identified Klebsiella PKBSG14 and analysis of its effect on immune response and cell cycle progression using common catfish (Channa punctatus) as a model.

Microb Pathog 2018 Dec 14. Epub 2018 Dec 14.

Parasitology Laboratory, Department of Zoology, University of Kalyani, 741235, West Bengal, India. Electronic address:

Microbial studies on Catfish revealed that Klebsiella is the most common pathogen causing prevalence of ulcers, fin erosion, and other lesions. During this study, a new strain of bacteria was isolated from Channa punctatus, and molecular identification by 16srRNA revealed the strain was Klebsiella PKBSG14 (Accession no KJ162158). The strain was also PCR positive for two virulent gene wcaG (Accession no LN606595) and rmpA (Accession no LN606594) responsible for inflammatory reactions and induction of innate immune response in the host cell. Read More

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December 2018
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