2,840,385 results match your criteria Molecular Cancer Therapeutics[Journal]


Methods for Assessing Mast Cell Responses to Engineered Nanomaterial Exposure.

Methods Mol Biol 2019 ;1894:31-45

Department of Pharmaceutical Sciences, Colorado Center for Nanomedicine and Nanosafety, Skaggs School of Pharmacy and Pharmaceutical Sciences, University of Colorado, Aurora, CO, USA.

Mast cells are key effector cells in inflammatory and allergic immune responses such as asthma, rhinitis, and atopic dermatitis. Activation of mast cells leads to immediate release of preformed mediators such as histamine and proteases, which can regulate vascular permeability and the function of a number of immune and nonimmune cells. Engineered nanomaterials (ENM) have been utilized for a wide range of applications and introduced into a number of consumer products; yet the consequent increase in human exposure and any potential adverse effects have not been fully evaluated. Read More

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January 2019

Using Drug Expression Profiles and Machine Learning Approach for Drug Repurposing.

Methods Mol Biol 2019 ;1903:219-237

School of Biomedical Sciences, The Chinese University of Hong Kong, Shatin, Hong Kong.

The cost of new drug development has been increasing, and repurposing known medications for new indications serves as an important way to hasten drug discovery. One promising approach to drug repositioning is to take advantage of machine learning (ML) algorithms to learn patterns in biological data related to drugs and then link them up to the potential of treating specific diseases. Here we give an overview of the general principles and different types of ML algorithms, as well as common approaches to evaluating predictive performances, with reference to the application of ML algorithms to predict repurposing opportunities using drug expression data as features. Read More

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January 2019

Implementation of a Pipeline Using Disease-Disease Associations for Computational Drug Repurposing.

Methods Mol Biol 2019 ;1903:129-148

Department of Biotechnology, School of Bio Sciences and Technology, Vellore Institute of Technology, Vellore, Tamil Nadu, India.

Drug repurposing is a powerful technique which has been recently employed in both industry and academia to discover and validate previously approved drugs for new indications. It provides the quickest possible transition from bench to bedside. In essence, computational strategies are appealing because they putatively nominate the most encouraging candidate for a given indication. Read More

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January 2019

A Computational Bipartite Graph-Based Drug Repurposing Method.

Methods Mol Biol 2019 ;1903:115-127

Institute of Medical Information/Library, Chinese Academy of Medical Sciences/Peking Union Medical College, Beijing, China.

We present a bipartite graph-based approach to calculate drug pairwise similarity for identifying potential new indications of approved drugs. Both chemical and molecular features were used in drug similarity calculation. In this paper, we first extracted drug chemical structures and drug-target interactions. Read More

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January 2019

Repurposing Drugs Based on Evolutionary Relationships Between Targets of Approved Drugs and Proteins of Interest.

Methods Mol Biol 2019 ;1903:45-59

Molecular Biophysics Unit, Indian Institute of Science, Bangalore, Karnataka, India.

Drug repurposing has garnered much interest as an effective method for drug development among biopharmaceutical companies. The availability of information on complete sequences of genomes and their associated biological data, genotype-phenotype-disease relationships, and properties of small molecules offers opportunities to explore the repurpose-able potential of existing pharmacopoeia. This method gains further importance, especially, in the context of development of drugs against infectious diseases, some of which pose serious complications due to emergence of drug-resistant pathogens. Read More

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January 2019

Performing an In Silico Repurposing of Existing Drugs by Combining Virtual Screening and Molecular Dynamics Simulation.

Methods Mol Biol 2019 ;1903:23-43

Department of Biology, Faculty of Science, Golestan University, Gorgan, Iran.

Drug repurposing has become one of the most widely used methods that can make drug discovery more efficient and less expensive. Additionally, computational methods such as structure-based drug designing can be utilized to make drug discovery more efficient and more accurate. Now imagine what can be achieved by combining drug repurposing and computational methods together in drug discovery, "in silico repurposing. Read More

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January 2019

Methods for Discovering and Targeting Druggable Protein-Protein Interfaces and Their Application to Repurposing.

Methods Mol Biol 2019 ;1903:1-21

Department of Chemical and Biological Engineering, Koc University, Istanbul, Turkey.

Drug repurposing is a creative and resourceful approach to increase the number of therapies by exploiting available and approved drugs. However, identifying new protein targets for previously approved drugs is challenging. Although new strategies have been developed for drug repurposing, there is broad agreement that there is room for further improvements. Read More

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January 2019

Prevalence and cardiac phenotype of patients with a phospholamban mutation.

Neth Heart J 2018 Dec 13. Epub 2018 Dec 13.

Department of Cardiology, University Medical Center Utrecht, Utrecht, The Netherlands.

Pathogenic mutations in the phospholamban (PLN) gene may give rise to inherited cardiomyopathies due to its role in calcium homeostasis. Several PLN mutations have been identified, with the R14del mutation being the most prevalent cardiomyopathy-related mutation in the Netherlands. It is present in patients diagnosed with arrhythmogenic cardiomyopathy as well as dilated cardiomyopathy. Read More

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December 2018

Gene Regulatory Networks: A Primer in Biological Processes and Statistical Modelling.

Methods Mol Biol 2019 ;1883:347-383

Institute of Fundamental Sciences, Palmerston North, New Zealand.

Modelling gene regulatory networks requires not only a thorough understanding of the biological system depicted, but also the ability to accurately represent this system from a mathematical perspective. Throughout this chapter, we aim to familiarize the reader with the biological processes and molecular factors at play in the process of gene expression regulation. We first describe the different interactions controlling each step of the expression process, from transcription to mRNA and protein decay. Read More

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January 2019

Integrative Approaches for Inference of Genome-Scale Gene Regulatory Networks.

Methods Mol Biol 2019 ;1883:161-194

Wisconsin Institute for Discovery, University of Wisconsin-Madison, Madison, WI, USA.

Transcriptional regulatory networks specify the regulatory proteins of target genes that control the context-specific expression levels of genes. With our ability to profile the different types of molecular components of cells under different conditions, we are now uniquely positioned to infer regulatory networks in diverse biological contexts such as different cell types, tissues, and time points. In this chapter, we cover two main classes of computational methods to integrate different types of information to infer genome-scale transcriptional regulatory networks. Read More

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January 2019

A Multiattribute Gaussian Graphical Model for Inferring Multiscale Regulatory Networks: An Application in Breast Cancer.

Methods Mol Biol 2019 ;1883:143-160

UMR MIA-Paris, AgroParisTech, INRA, Université Paris-Saclay, Paris, France.

This chapter addresses the problem of reconstructing regulatory networks in molecular biology by integrating multiple sources of data. We consider data sets measured from diverse technologies all related to the same set of variables and individuals. This situation is becoming more and more common in molecular biology, for instance, when both proteomic and transcriptomic data related to the same set of "genes" are available on a given cohort of patients. Read More

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January 2019

Statistical Network Inference for Time-Varying Molecular Data with Dynamic Bayesian Networks.

Methods Mol Biol 2019 ;1883:25-48

German Center for Neurodegenerative Diseases (DZNE), Bonn, Germany.

In this chapter, we review the problem of network inference from time-course data, focusing on a class of graphical models known as dynamic Bayesian networks (DBNs). We discuss the relationship of DBNs to models based on ordinary differential equations, and consider extensions to nonlinear time dynamics. We provide an introduction to time-varying DBN models, which allow for changes to the network structure and parameters over time. Read More

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January 2019

Multiplex PCR for the rapid detection of insulin-like growth factor in the Pacific oyster, Crassostrea gigas: a useful indicator for growth assessment.

Mol Biol Rep 2018 Dec 13. Epub 2018 Dec 13.

Department of Marine Bio-Materials & Aquaculture, Pukyong National University, Busan, 48513, South Korea.

Insulin-like growth factor (IGF) expression plays a critical role in the endocrine regulation of proliferation, differentiation, and growth in shellfish as well as in fish. The Pacific oyster, Crassostrea gigas, is a significant aquaculture species that comprises a large percentage of the Korean shellfish industry; moreover, its growth is economically important in aquaculture. However, when measuring the growth rate in shellfish, the soft tissue weight is difficult to determine because of the shell weight. Read More

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December 2018

Genetic diversity between two Egyptian clover varieties and QTL analysis for some agro-morphological traits.

Mol Biol Rep 2018 Dec 13. Epub 2018 Dec 13.

Department of Agronomy, Faculty of Agriculture, Assiut University, Assiut, 71526, Egypt.

Genetic diversity between two ecotypes of Egyptian clover varieties, namely Fahl (mono-cut) and Helaly (multi-cut) have been assessed based on forage yield and yield components as well as molecular marker systems. The two parental genotypes were crossed to produce seeds of F and F progenies. Analyses of variance indicated significant differences between four populations (P (Fahl), P (Helaly), F and F) for fresh forage yield, number of florets/inflorescence, number of seeds/inflorescence and 1000 seed weight. Read More

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December 2018

Imaging of Bronchial Pathology in Antibody Deficiency: Data from the European Chest CT Group.

J Clin Immunol 2018 Dec 13. Epub 2018 Dec 13.

Paediatric Immunology Unit, Department of Paediatric Pulmonology, Allergology and Neonatology, Hanover Medical School, Carl-Neuberg Str. 1, 30625, Hannover, Germany.

Studies of chest computed tomography (CT) in patients with primary antibody deficiency syndromes (ADS) suggest a broad range of bronchial pathology. However, there are as yet no multicentre studies to assess the variety of bronchial pathology in this patient group. One of the underlying reasons is the lack of a consensus methodology, a prerequisite to jointly document chest CT findings. Read More

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December 2018
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Chromoblastomycosis Caused by Fonsecaea nubica: First Report in Northern China and Literature Review.

Mycopathologia 2018 Dec 13. Epub 2018 Dec 13.

Department of Dermatovenereology, West China Hospital, Sichuan University, No. 37, Guo Xue Xiang, Wuhou District, Chengdu, 610041, Sichuan Province, China.

Chromoblastomycosis is found worldwide with higher incidence in tropical and subtropical regions. Fonsecaea spp. is one of the major causative agents of this disease. Read More

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December 2018

Eotaxins and C-C chemokine receptor type 3 in Parkinson's disease.

Acta Neurol Belg 2018 Dec 13. Epub 2018 Dec 13.

Geriatric Care Research Center, Rafsanjan University of Medical Science, Rafsanjan, Iran.

Parkinson's disease (PD) is one of the most common neuroinflammatory disorders and inflammatory processes seem to play an important role in the pathogenesis of PD. Chemokines as inflammatory mediators, which are involved in the recruitment of leukocytes, can play a role in the pathogenesis of PD. The aim of this study was to examine the serum level of eotaxins (CCL11, CCL24, and CCL26) and the expression of C-C chemokine receptor type 3 (CCR3) in patients with PD compared with healthy subjects. Read More

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December 2018

Functional significance of channelopathy gene variants in unexplained death.

Forensic Sci Med Pathol 2018 Dec 13. Epub 2018 Dec 13.

Pediatrics, NYU School of Medicine, 450 E 29th Street, ACLS 824, New York, NY, 10016, USA.

Determining the cause of unexplained death in all age groups, including infants, is a priority in forensic medicine. The triple risk model proposed for sudden infant death syndrome involves the intersection of three risks: (1) a critical developmental period in homeostatic control (2), exogenous stressors, and (3) a vulnerable infant. Even though sex and age factor into some forms of inherited arrhythmogenic deaths in young individuals and adults, more appropriate a dual-risk disease model for adults involves exogenous stressors and a vulnerable individual. Read More

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December 2018
3 Reads

Insomnia and cardiorespiratory fitness in a middle-aged population: the SCAPIS pilot study.

Sleep Breath 2018 Dec 13. Epub 2018 Dec 13.

Center for Sleep and Vigilance Disorders, Department of Internal Medicine and Clinical Nutrition, Sahlgrenska Academy, University of Gothenburg, Medicinaregatan 8b, Box 421, SE-40530, Gothenburg, Sweden.

Background: The relationship between insomnia and cardiorespiratory fitness (CRF), a well-established risk factor for cardiovascular disease, has not been extensively studied. We aimed to assess the independent association between insomnia and CRF in a population-based cohort of subjects aged 50 to 64 years.

Methods: Subjects participating in the Swedish CArdioPulmonary bioImaging Study (SCAPIS) pilot cohort (n = 603, men 47. Read More

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December 2018

A review of filamin A mutations and associated interstitial lung disease.

Eur J Pediatr 2018 Dec 13. Epub 2018 Dec 13.

Clinical Genetics Department, Our Lady's Children's Hospital, Dublin, Ireland.

The filamin A gene (FLNA) on Xq28 encodes the filamin A protein. Mutation in FLNA causes a wide spectrum of disease including skeletal dysplasia, neuronal migration abnormality, cardiovascular malformation, intellectual disability and intestinal obstruction. Recently, childhood-onset interstitial lung disease associated with a range of FLNA mutations has been recognised and reported. Read More

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December 2018

Advances in quantitative assessment of parkinsonian motor symptoms with wearable devices.

Sci China Life Sci 2018 Dec 3. Epub 2018 Dec 3.

Department of Neurology, Fujian Institute of Geriatrics, Fujian Medical University Union Hospital, Fuzhou, 350001, China.

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December 2018

The bacteriology in adult patients with pneumonia and parapneumonic effusions: increased yield with DNA sequencing method.

Eur J Clin Microbiol Infect Dis 2018 Dec 13. Epub 2018 Dec 13.

Department of Medicine, Solna, Infectious Diseases Unit, Karolinska University Hospital, Karolinska Institutet, Stockholm, Sweden.

The aim of this study was to use a 16S rDNA sequencing method in combination with conventional culture in patients with parapneumonic effusions (PPE) to evaluate the methods, study the microbiological spectrum, and examine the presence of bacteria within the different stages of PPE. Adults with community-acquired pneumonia (CAP) and PPE (n = 197) admitted to the Departments of Infectious Diseases at four hospitals in Stockholm County during 2011-2014 were prospectively studied. All patients underwent thoracentesis. Read More

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December 2018

Early Hereditary Diffuse Gastric Cancer (eHDGC) is Characterized by Subtle Genomic Instability and Active DNA Damage Response.

Pathol Oncol Res 2018 Dec 13. Epub 2018 Dec 13.

Division of Biomedical Science and Biochemistry, Research School of Biology, ANU College of Medicine, Biology and Environment, Australian National University, P.O. Box: 2601, Canberra, Australia.

Diffuse gastric cancer (DGC) is one of the two primary types of stomach cancer. Carriers of germline mutations in the gene encoding E-cadherin are predisposed to DGC. The primary aim of the present study was to determine if genomic instability is an early event in DGC and how it may lead to disease progression. Read More

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December 2018

Structure, Molecular Modification, and Anti-radiation Activity of Melanin from Lachnum YM156 on Ultraviolet B-Induced Injury in Mice.

Appl Biochem Biotechnol 2018 Dec 14. Epub 2018 Dec 14.

School of Food and Biological Engineering, Hefei University of Technology, Hefei, 230009, China.

The purpose of this study was to examine the protective effects of intracellular homogeneous melanin produced by Lachnum YM156 (LIM) against ultraviolet B (UVB) induced damage in mice. The possible structural formula of the LIM was concluded based on elemental analysis, ultraviolet-visible spectroscopy (UV-Vis), Fourier transform infrared spectroscopy (FTIR), nuclear magnetic resonance (NMR), and mass spectrometry (MS). The LIM was modified by arginine to improve its water solubility and biological activities. Read More

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December 2018

Isolation of living apical and basal cell lineages of early proembryos for transcriptome analysis.

Plant Reprod 2018 Dec 13. Epub 2018 Dec 13.

State Key Laboratory of Hybrid Rice, College of Life Sciences, Wuhan University, Wuhan, 430072, China.

Plant zygotes usually undergo asymmetrical cell division, giving rise to the formation of two daughter cells with distinct developmental cell fate. The small apical cell will develop into the major part of embryo proper, whereas the larger basal cell will divide to form a transient suspensor. Thus, the apical and basal cell lineages are an excellent model to study cell fate determination in relation to zygote polarity. Read More

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December 2018

Glucose metabolic brain patterns to discriminate amyotrophic lateral sclerosis from Parkinson plus syndromes.

EJNMMI Res 2018 Dec 13;8(1):110. Epub 2018 Dec 13.

Department of Nuclear Medicine and Molecular Imaging, Division of Nuclear Medicine, KU Leuven, Herestraat 49, 3000, Leuven, Belgium.

Background: F-FDG brain PET measures metabolic changes in neurodegenerative disorders and may discriminate between different diseases even at an early stage. The objective of this study was to classify patients with amyotrophic lateral sclerosis (ALS) and Parkinson plus syndromes (PP). To this end, different approaches were evaluated using generalized linear models and corresponding glucose metabolic brain patterns. Read More

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December 2018

Cyanase-independent utilization of cyanate as a nitrogen source in ascomycete yeasts.

Authors:
Tomas Linder

World J Microbiol Biotechnol 2018 Dec 13;35(1). Epub 2018 Dec 13.

Department of Molecular Sciences, Swedish University of Agricultural Sciences, Box 7015, 750 07, Uppsala, Sweden.

The occurrence of putative cyanases (EC 4.2.1. Read More

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December 2018
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Bone marrow cell homing to sites of acute tibial fracture: Zr-oxine cell labeling with positron emission tomographic imaging in a mouse model.

EJNMMI Res 2018 Dec 13;8(1):109. Epub 2018 Dec 13.

Molecular Imaging Program, Center for Cancer Research, National Cancer Institute, National Institutes of Health, NIH, Building 10, Room B3B406, Bethesda, MD, 20892-1002, USA.

Background: Bone fracture healing is dependent upon the rapid migration and engraftment of bone marrow (BM) progenitor and stem cells to the site of injury. Stromal cell-derived factor-1 plays a crucial role in recruiting BM cells expressing its receptor CXCR4. Recently, a CXCR4 antagonist, plerixafor, has been used to mobilize BM cells into the blood in efforts to enhance cell migration to sites of injury presumably improving healing. Read More

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December 2018

A novel rare CUBN variant and three additional genes identified in Europeans with and without diabetes: results from an exome-wide association study of albuminuria.

Diabetologia 2018 Dec 13. Epub 2018 Dec 13.

Novo Nordisk Foundation Center for Basic Metabolic Research, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark.

Aims/hypothesis: Identifying rare coding variants associated with albuminuria may open new avenues for preventing chronic kidney disease and end-stage renal disease, which are highly prevalent in individuals with diabetes. Efforts to identify genetic susceptibility variants for albuminuria have so far been limited, with the majority of studies focusing on common variants.

Methods: We performed an exome-wide association study to identify coding variants in a two-stage (discovery and replication) approach. Read More

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December 2018

Use of human islets to understand islet biology and diabetes: progress, challenges and suggestions.

Diabetologia 2018 Dec 14. Epub 2018 Dec 14.

Department of Medicine, Division of Diabetes, Endocrinology, and Metabolism, 7465 Medical Research Bldg IV, Vanderbilt University Medical Center, 2215 Garland Avenue, Nashville, TN, 37232-0475, USA.

Over the last two decades, improved access to human islets and the development of human islet distribution networks have enabled the use of millions of human islets in hundreds of scientific research projects, leading to a dramatic increase in our understanding of human islet biology. Here we discuss recent scientific advances as well as methodological and experimental challenges that impact human islet quality, experimental outcomes and the reporting of human islets used in scientific publications. In a survey of over 200 scientific publications with human islet experimentation, we found that the reporting of critical information was quite variable, sometimes obscure, and often failed to adequately outline the experiments and results using human islets. Read More

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December 2018

Increases in cytosolic Ca induce dynamin- and calcineurin-dependent internalisation of CFTR.

Cell Mol Life Sci 2018 Dec 13. Epub 2018 Dec 13.

Institute for Cell and Molecular Biosciences, Newcastle University, Newcastle upon Tyne, NE2 4HH, UK.

The cystic fibrosis transmembrane conductance regulator (CFTR) is a cAMP-regulated, apical anion channel that regulates ion and fluid transport in many epithelia including the airways. We have previously shown that cigarette smoke (CS) exposure to airway epithelia causes a reduction in plasma membrane CFTR expression which correlated with a decrease in airway surface hydration. The effect of CS on CFTR was dependent on an increase in cytosolic Ca. Read More

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December 2018

Biochemical characterization and low-resolution SAXS structure of two-domain endoglucanase BlCel9 from Bacillus licheniformis.

Appl Microbiol Biotechnol 2018 Dec 13. Epub 2018 Dec 13.

Instituto de Física de São Carlos, Universidade de São Paulo, Av. Trabalhador Saocarlense 400, São Carlos, SP, 13560-970, Brazil.

Lignocellulose feedstock constitutes the most abundant carbon source in the biosphere; however, its recalcitrance remains a challenge for microbial conversion into biofuel and bioproducts. Bacillus licheniformis is a microbial mesophilic bacterium capable of secreting a large number of glycoside hydrolase (GH) enzymes, including a glycoside hydrolase from GH family 9 (BlCel9). Here, we conducted biochemical and biophysical studies of recombinant BlCel9, and its low-resolution molecular shape was retrieved from small angle X-ray scattering (SAXS) data. Read More

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December 2018

Correction to: Morphological MRI-based features provide pretreatment survival prediction in glioblastoma.

Eur Radiol 2018 Dec 13. Epub 2018 Dec 13.

Mathematical Oncology Laboratory (MôLAB), Department of Mathematics, Universidad de Castilla-La Mancha, 13071, Ciudad Real, Spain.

The original version of this article, published on 15 October 2018, unfortunately contained a mistake. The following correction has therefore been made in the original: The name of Mariano Amo-Salas and the affiliation of Ismael Herruzo were presented incorrectly. Read More

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December 2018

SIU-ICUD consultation on bladder cancer: basic science.

World J Urol 2018 Dec 13. Epub 2018 Dec 13.

Scott Department of Urology, Dan L. Duncan Cancer Center, Baylor College of Medicine, Houston, TX, USA.

Purpose: To provide a condensed summary of the Basic Science chapter that was included in the Third International Consultation on Bladder Cancer.

Methods: World bladder cancer basic science experts used the published literature to create summaries of recent progress in their areas of expertise.

Results: The completion of several large-scale genomics projects coupled with a strong collaborative culture within the research community and the exciting clinical activity of immune checkpoint blockade have combined to transform the bladder cancer research landscape. Read More

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December 2018

Breaking the curse of dimensionality to identify causal variants in Breeding 4.

Theor Appl Genet 2018 Dec 13. Epub 2018 Dec 13.

Institute for Genomic Diversity, Institute of Biotechnology, Cornell University, 175 Biotechnology Building, Ithaca, NY, 14853, USA.

In the past, plant breeding has undergone three major transformations and is currently transitioning to a new technological phase, Breeding 4. This phase is characterized by the development of methods for biological design of plant varieties, including transformation and gene editing techniques directed toward causal loci. The application of such technologies will require to reliably estimate the effect of loci in plant genomes by avoiding the situation where the number of loci assayed (p) surpasses the number of plant genotypes (n). Read More

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December 2018
2 Reads

Characterisation of barley resistance to rhynchosporium on chromosome 6HS.

Theor Appl Genet 2018 Dec 13. Epub 2018 Dec 13.

The James Hutton Institute, Invergowrie, Dundee, DD2 5DA, Scotland, UK.

Key Message: Major resistance gene to rhynchosporium, Rrs18, maps close to the telomere on the short arm of chromosome 6H in barley. Rhynchosporium or barley scald caused by a fungal pathogen Rhynchosporium commune is one of the most destructive and economically important diseases of barley in the world. Testing of Steptoe × Morex and CIho 3515 × Alexis doubled haploid populations has revealed a large effect QTL for resistance to R. Read More

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December 2018

Improving NanoCluster Beacon performance by blocking the unlabeled NC probes.

Chem Commun (Camb) 2018 Dec 14. Epub 2018 Dec 14.

Department of Biomedical Engineering, University of Texas at Austin, Austin, Texas 78712, USA. and Texas Materials Institute, University of Texas at Austin, Austin, Texas 78712, USA.

While NanoCluster Beacon (NCB) is a versatile molecular probe, it suffers from a low target-specific signal issue due to impurities. Here we show that adding a "blocker" strand to the reaction can effectively block the nonfunctional probes and enhance the target-specific signal by 14 fold at a 0.1 target/probe ratio. Read More

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December 2018

AuNP-peptide probe for caspase-3 detection in living cells by SERS.

Analyst 2018 Dec 14. Epub 2018 Dec 14.

State Key Laboratory of Food Nutrition and Safety, Tianjin University of Science and Technology, 300457 Tianjin, China. and Research Centre of Food Science and Human Health, School of Medicine, Nankai University, 300071 Tianjin, China.

Colloidal nanoparticles can be used as surface-enhanced Raman scattering (SERS) substrates because the very close spacing between particles existing in these colloidal systems is beneficial for the generation of extremely strong and highly spatially localized electric field enhancements. Herein, a caspase-3-specified peptide was used as a molecular cross-linker to engineer gold nanoparticle (AuNP) junctions in a controllable manner. The peptide was designed with a sequence of CCALNNPFFDVED (Cys-Cys-Ala-Leu-Asn-Asn-Pro-Phe-Phe-Asp-Val-Glu-Asp) or CCALNNKYDDVED (Cys-Cys-Ala-Leu-Asn-Asn-Lys-Tyr-Asp-Asp-Val-Glu-Asp), where the CALNN (Cys-Ala-Leu-Asn-Asn) fragment helps to stabilize AuNP suspension in aqueous media and the sequence of DVED (Asp-Glu-Val-Asp) can be cleaved by caspase-3. Read More

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December 2018

Elucidating cation-cation interactions in neptunyl dications using multi-reference ab initio theory.

Phys Chem Chem Phys 2018 Dec 14. Epub 2018 Dec 14.

Institute of Physics, Faculty of Physics, Astronomy and Informatics, Nicolaus Copernicus University in Toruń, Grudziądzka 5, 87-100 Toruń, Poland.

Understanding the binding mechanism in neptunyl clusters formed due to cation-cation interactions is of crucial importance in nuclear waste reprocessing and related areas of research. Since experimental manipulations with such species are often rather limited, we have to rely on quantum-chemical predictions of their electronic structures and spectroscopic parameters. In this work, we present a state-of-the-art quantum chemical study of the T-shaped and diamond-shaped neptunyl(v) and neptunyl(vi) dimers. Read More

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December 2018

FeO-ZIF-8 assemblies as pH and glutathione responsive T-T switching magnetic resonance imaging contrast agent for sensitive tumor imaging in vivo.

Chem Commun (Camb) 2018 Dec 14. Epub 2018 Dec 14.

The Key Laboratory of Resource Chemistry of Ministry of Education, Shanghai Key Laboratory of Rare Earth Functional Materials, and Shanghai Municipal Education Committee Key Laboratory of Molecular Imaging Probes and Sensors, Shanghai Normal University, Shanghai 200234, China.

A small Fe3O4 nanoparticles-based T1 contrast agent was assembled into a pH- and glutathione-responsive T2-T1 switching contrast agent, Fe3O4-ZIF-8. In vivo T1-weighted images of mice showed that Fe3O4-ZIF-8 displayed darkening contrast enhancement for liver sites, while darkening to brightening contrast enhancement at tumor sites, giving large inverse contrast for distinguishing the normal and tumor tissues. Read More

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December 2018

Visible-light mediated carbamoyl radical addition to heteroarenes.

Chem Commun (Camb) 2018 Dec 14. Epub 2018 Dec 14.

University of Bordeaux, Institute of Molecular Sciences (ISM), UMR-CNRS 5255, 351, Cours de la Libération, 33405 Talence Cedex, France.

The generation of carbamoyl radicals, followed by their addition to heteroarenes, was performed under mild conditions through a metal-free photocatalyzed decarboxylation of oxamic acids. The process has been applied to the carbamoylation of heteroaromatic bases using α-aminoacid-derived oxamic acids, leading to the corresponding amides without racemization. Read More

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December 2018

Successful treatment of disseminated intravascular coagulation associated with aortic dissection.

J Vasc Surg Cases Innov Tech 2018 Dec 4;4(4):268-271. Epub 2018 Dec 4.

Division of Cardiovascular Surgery, Narita Red Cross Hospital, Chiba, Japan.

Disseminated intravascular coagulation (DIC) is an infrequent aortic dissection complication, and its optimal treatment remains controversial. A 55-year-old woman developed DIC associated with Stanford type B aortic dissection, which improved by administration of low-molecular-weight heparin combined with tranexamic acid, but the dissecting aneurysm of the descending aorta was dilated. After thoracic endovascular aortic repair for occlusion of entry tears detected by transesophageal echocardiography, DIC improved without anticoagulant therapy. Read More

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December 2018

Thyroid Hormone Deiodination and Action in the Gonads.

Authors:
Arturo Hernandez

Curr Opin Endocr Metab Res 2018 Oct 7;2:18-23. Epub 2018 Feb 7.

Maine Medical Center Research Institute, Center for Molecular Medicine, Scarborough, Maine, USA.

Alterations in thyroid hormone (TH) status during developmental or adult age are associated with abnormal gonadal development and function in human and animal models. In the developing testis, aberrant levels of TH action lead to disruptions in cell proliferation and differentiation, ultimately influencing testicular size, spermatogenesis, steroidogenesis and male fertility. Recent work is increasingly identifying the systems and signaling pathways in gonadal cells that are affected by TH, as well as delineating the major factors that determine TH signaling in gonadal tissue. Read More

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October 2018

Subregional Hippocampal Thickness Abnormalities in Older Adults with a History of Heavy Cannabis Use.

Cannabis Cannabinoid Res 2018 10;3(1):242-251. Epub 2018 Dec 10.

Semel Institute for Neuroscience and Human Behavior, University of California, Los Angeles, California.

Legalization of cannabis (CB) for both medicinal and, in some states, recreational use, has given rise to increasing usage rates across the country. Of particular concern are indications that frequent CB use may be selectively harmful to the developing adolescent brain compared with adult-onset usage. However, the long-term effects of heavy, adolescent CB use on brain structure and cognitive performance in late-life remain unknown. Read More

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December 2018
1 Read

Neuroinflammatory signals drive spinal curve formation in zebrafish models of idiopathic scoliosis.

Sci Adv 2018 Dec 12;4(12):eaav1781. Epub 2018 Dec 12.

Program in Developmental & Stem Cell Biology, The Hospital for Sick Children, 686 Bay Street, Toronto, Ontario M5G 0A4, Canada.

The etiopathogenesis of idiopathic scoliosis (IS), a highly prevalent spinal deformity that occurs in the absence of obvious congenital or physiological abnormalities, is poorly understood. Although recent zebrafish genetic studies have linked cilia motility and cerebrospinal fluid (CSF) flow defects with scoliosis progression, underlying mechanisms were not identified. Here, we use next-generation sequencing and conditional genetic methodologies to define the spatial and biological origins of spinal curve formation in mutant zebrafish, a faithful IS model. Read More

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December 2018

Drug-Drug Interaction Discovery: Kernel Learning from Heterogeneous Similarities.

Smart Health (Amst) 2018 Dec 7;9-10:88-100. Epub 2018 Jul 7.

Erik Jonsson School of Engineering and Computer Science, The University of Texas at Dallas, United States.

We develop a pipeline to mine complex drug interactions by combining different similarities and interaction types (molecular, structural, phenotypic, genomic etc). Our goal is to learn an optimal kernel from these heterogeneous similarities in a supervised manner. We formulate an extensible framework that can easily integrate new interaction types into a rich model. Read More

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December 2018

Draft genome sequence of the moderately halophilic bacterium sp. BBL2006.

Data Brief 2018 Dec 17;21:2410-2413. Epub 2018 Nov 17.

Department of Biology, Indiana University Southeast, New Albany, IN, USA.

We present the draft genome sequence of sp. BBL2006, a moderately halophilic, gram positive bacterium isolated from a sulfidic salt spring in Big Bone Lick State Park, Boone County, Kentucky. The genome of sp. Read More

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December 2018

Lentiviral Vector Purification Using Genetically Encoded Biotin Mimic in Packaging Cell.

Mol Ther Methods Clin Dev 2018 Dec 23;11:155-165. Epub 2018 Oct 23.

UCL Cancer Institute, University College London, Paul O'Gorman Building, 72 Huntley Street, London WC1E 6BT, UK.

Lentiviral vectors (LVs) have recently witnessed an increasing demand in research and clinical applications. Their current purification processes represent the main bottleneck in their widespread use, as the methods used are cumbersome and yield low recoveries. We aimed to develop a one-step method to specifically purify LVs, with high yields and reduced levels of impurities, using the biotin-streptavidin system. Read More

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December 2018

Alternative Splicing Regulator RBM20 and Cardiomyopathy.

Front Mol Biosci 2018 28;5:105. Epub 2018 Nov 28.

Laboratory of Gene Expression, Medical Research Institute, Tokyo Medical and Dental University (TMDU), Tokyo, Japan.

RBM20 is a vertebrate-specific RNA-binding protein with two zinc finger (ZnF) domains, one RNA-recognition motif (RRM)-type RNA-binding domain and an arginine/serine (RS)-rich region. has initially been identified as one of dilated cardiomyopathy (DCM)-linked genes. RBM20 is a regulator of heart-specific alternative splicing and mice lacking the RRM domain are defective in the splicing regulation. Read More

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November 2018

Biochemical Characterization of AroH, a Putative Aromatic Amino Acid Aminotransferase.

Front Mol Biosci 2018 28;5:104. Epub 2018 Nov 28.

Department of Experimental Medicine, University of Perugia, Perugia, Italy.

The rise in the frequency of nosocomial infections is becoming a major problem for public health, in particular in immunocompromised patients. is an opportunistic fungus normally present in the environment directly responsible for lethal invasive infections. Recent results suggest that the metabolic pathways related to amino acid metabolism can regulate the fungus-host interaction and that an important role is played by enzymes involved in the catabolism of L-tryptophan. Read More

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November 2018
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