6,895 results match your criteria Molecular Biology Reports [Journal]


Comparison of the effect of three different topoisomerase II inhibitors combined with cisplatin in human glioblastoma cells sensitized with double strand break repair inhibitors.

Mol Biol Rep 2019 Apr 24. Epub 2019 Apr 24.

Department of Molecular Genetics, Faculty of Biology and Environmental Protection, University of Lodz, Pomorska 141/143, Lodz, 90-236, Poland.

Topoisomerase II (Topo2) inhibitors in combination with cisplatin represent a common treatment modality used for glioma patients. The main mechanism of their action involves induction of DNA double-strand breaks (DSBs). DSBs are repaired via the homology-dependent DNA repair (HRR) and non-homologous end-joining (NHEJ). Read More

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http://dx.doi.org/10.1007/s11033-019-04605-0DOI Listing

Recombinant production of a bioactive peptide from spotless smooth-hound (Mustelus griseus) muscle and characterization of its antioxidant activity.

Mol Biol Rep 2019 Apr 24. Epub 2019 Apr 24.

Genetics and Agricultural Biotechnology Institute of Tabarestan, Sari Agricultural Sciences and Natural Resources University, Sari, Iran.

Bioactive peptides are short amino acid sequences with desirable health effects which are derived from animals, plants, and marine sources. In this study, recombinant production of a bioactive peptide (GIISHR) from spotless smooth-hound (Mustelus griseus) muscle and its antioxidant properties is discussed. A gene composed of 12 tandem copies of the peptide sequence was cloned in pET-28a and expressed as a His-tagged polypeptide in Escherichia coli. Read More

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http://link.springer.com/10.1007/s11033-018-4468-1
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http://dx.doi.org/10.1007/s11033-018-4468-1DOI Listing
April 2019
1 Read

Resolving the pathogenicity factors of a novel opportunistic fungus Schizophyllum commune at molecular level.

Mol Biol Rep 2019 Apr 23. Epub 2019 Apr 23.

SRM Research Institute, SRM Institute of Science and Technology, Kattankulathur, Chennai, Tamil Nadu, 603 203, India.

Schizophyllum commune is a well-known mushroom forming fungi which is an edible one due to its nutritive value. It exhibits a special wood degrading mechanism to grow in decay matters by releasing a series of enzymes. These enzymes might make them an opportunistic pathogen which has been reported to infect various animals and human beings too. Read More

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http://dx.doi.org/10.1007/s11033-019-04830-7DOI Listing

Selection of suitable reference genes for core clock gene expression analysis by real-time qPCR in rat ovary granulosa cells.

Mol Biol Rep 2019 Apr 23. Epub 2019 Apr 23.

College of Animal Science and Technology, Northwest Agriculture and Forestry University, Yangling, Shaanxi, 712100, People's Republic of China.

Selection of a suitable endogenous reference gene is essential for investigating expression of clock genes Bmal1, Clock, Pers, Crys, Rev-erbα/β, and RORα/β/γ involved in the circadian system. In this study, we treated rat ovary granulosa cells with dexamethasone to synchronize circadian oscillation in vitro and determined expression levels of Bmal1 and Per2 and six candidate reference genes (Actb, Beta actin; B2m, Beta-2-microglobulin; Ppia, Cyclophilin A; Gapdh, Glyceraldehyde-3-phosphate dehydrogenase; Hprt, Hypoxanthine guanine phosphoribosyl transferase and Tbp, TATA-box-binding protein) using quantitative real-time PCR. We then employed three software programs, GeNorm, NormFinder, and BestKeeper, to analyze the expression data for the selection of the best reference gene. Read More

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http://dx.doi.org/10.1007/s11033-019-04755-1DOI Listing

Behavioural and biochemical parameters in guppy (Poecilia vivipara) following exposure to waterborne zinc in salt or hard water.

Mol Biol Rep 2019 Apr 23. Epub 2019 Apr 23.

Programa de Pós-Graduação em Ciências Biológicas, Bioquímica Toxicológica, Universidade Federal de Santa Maria (UFSM), Avenida Roraima, 1000, Santa Maria, RS, 97015-900, Brazil.

Zinc is an essential trace mineral that is involved in many biological processes. In elevated concentrations, this metal may have toxic effects for aquatic organisms. Physicochemical properties of water, such as salinity and hardness, can influence the bioavailability of zinc and, therefore its toxicity in aquatic environments. Read More

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http://dx.doi.org/10.1007/s11033-019-04802-xDOI Listing

Lentiviral-mediated delivery of classical swine fever virus Erns gene into porcine kidney-15 cells for production of recombinant ELISA diagnostic antigen.

Mol Biol Rep 2019 Apr 23. Epub 2019 Apr 23.

Division of Veterinary Biotechnology, Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh, 243122, India.

Classical swine fever virus (CSFV), a member of the Pestivirus genus within the Flaviviridae family causes contagious fatal disease in swine. Antibodies against E2, Erns and NS3 proteins of virus can be detected in infected animals. Development of an ELISA coating antigen to improve the sensitivity of detecting Erns-specific antibodies in pig sera is always desirable for diagnosis as well as for differentiation of infected from vaccinated animals. Read More

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http://dx.doi.org/10.1007/s11033-019-04829-0DOI Listing

Association of vascular endothelial growth factor (VEGF) Gene polymorphisms and expression with the risk of endometriosis: a case-control study.

Mol Biol Rep 2019 Apr 22. Epub 2019 Apr 22.

Metabolic Disorders Research Center, Endocrinology and Metabolism Molecular-Cellular Sciences Institute, Tehran University of Medical Sciences, 5th Floor, Shariati Hospital Complex, North Kargar Ave, Tehran, 1411713135, Iran.

Endometriosis is a polygenic and multifactorial gynecology situation which might be associated with angiogenesis. In the current study we assess the role of vascular endothelial growth factor (VEGF) - 2578 A/C, and + 936 C/T polymorphisms in susceptibility to endometriosis and checking the expression of VEGF mRNA in eutopic tissue of endometrium with and without endometriosis. The study was comprised of 300 patients who underwent laparascopic or laparotomy surgery with 100 cases who had confirmed histological diagnosis of endometriosis, and 200 controls with no histological diagnosis of disease. Read More

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http://dx.doi.org/10.1007/s11033-019-04807-6DOI Listing
April 2019
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Differential abundance and core members of the bacterial community associated with wild male Zeugodacus cucurbitae fruit flies (Insecta: Tephritidae) from three geographical regions of Southeast Asia.

Mol Biol Rep 2019 Apr 22. Epub 2019 Apr 22.

Institute of Ocean and Earth Sciences, University of Malaya, 50603, Kuala Lumpur, Malaysia.

Zeugodacus cucurbitae (Coquillet) is one of the most significant and widespread tephritid pest species of agricultural crops. This study reports the bacterial communities associated with Z. cucurbitae from three geographical regions in Southeast Asia (Thailand, Peninsular Malaysia, and Sarawak). Read More

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http://dx.doi.org/10.1007/s11033-019-04818-3DOI Listing

Erythrocyte binding ligand region VI specific IgA confers tissue protection in malaria infection.

Mol Biol Rep 2019 Apr 22. Epub 2019 Apr 22.

National Centre for Cell Science, Savitribai Phule Pune University Campus, Ganeshkhind Road, Pune, 411007, India.

A direct role for IgA either for elimination of malaria parasite or for improvement in tissue pathology has not been investigated in case of Malaria infection while IgG, IgE and IgM were all implicated in the adverse pathology. In this communication, we delineate further that Malaria specific IgA appears to be significant among individuals who had multiple episodes of infection. Interestingly, the IgA elicited by immunization of the homologous peptides derived from Plasmodium berghei ANKA have also resulted in protection of host from adverse lung pathology, while the parasite load is unaffected. Read More

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http://dx.doi.org/10.1007/s11033-019-04822-7DOI Listing

Conserved miRNAs modulate the expression of potential transcription factors of isoflavonoid biosynthetic pathway in soybean seeds.

Mol Biol Rep 2019 Apr 22. Epub 2019 Apr 22.

Division of Biochemistry, ICAR-Indian Agricultural Research Institute, Pusa Campus, New Delhi, 110 012, India.

Despite the significant importance of soybean isoflavone, the regulatory mechanism of miRNAs during its biosynthesis is highly unexplored. In the present work, nine existing miRNAs along with their ten corresponding target genes were identified and validated in soybean for their possible role during isoflavonoid biosynthesis and accumulation. Temporal expression analysis at four key stages of seed development (35, 45, 55 and 65DAF) of all the miRNA-target pairs showed varying degree of differential accumulation in two soybean genotypes (NRC37: high isoflavone; and NRC7: low isoflavone). Read More

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http://dx.doi.org/10.1007/s11033-019-04814-7DOI Listing

Strain identification and metabolites isolation of Aspergillus capensis CanS-34A from Brassica napus.

Mol Biol Rep 2019 Apr 22. Epub 2019 Apr 22.

State Key Laboratory of Agricultural Microbiology and Key Laboratory of Plant Pathology of Hubei Province, Huazhong Agricultural University, Wuhan, 430070, China.

An isolate (CanS-34A) of Aspergillus from a healthy plant of oilseed rape (Brassica napus) was identified based on morphological characterization and multi-locus phylogeny using the sequences of internal transcribed spacer (ITS)-5.8S rDNA region, BenA (for β-tubulin), CaM (for calmodulin) and RPB2 (for RNA polymerase II). The results showed that CanS-34A belongs to Aspergillus capensis Hirooka et al. Read More

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http://dx.doi.org/10.1007/s11033-019-04808-5DOI Listing

Identification and sequencing of the gene encoding DNA methyltransferase 3 (DNMT3) from sea cucumber, Apostichopus japonicus.

Mol Biol Rep 2019 Apr 20. Epub 2019 Apr 20.

School of Biological Sciences and Technology, Chonnam National University, Gwangju, 61186, Republic of Korea.

The sea cucumber Apostichopus japonicus is well known as a traditional tonic food and as a commercially important cultured aquatic species. This species produces saponins, and has a high potential to cope with environmental stress, such as aestivation, organ regeneration, and wound healing. Recently, several studies have shown that cellular reprogramming and the physiological responses of the sea cucumber to environmental changes, including aestivation, are potentially mediated by epigenetic DNA methylation. Read More

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http://dx.doi.org/10.1007/s11033-019-04821-8DOI Listing

Comparative transcriptome profiling of resistant and susceptible sugarcane genotypes in response to the airborne pathogen Fusarium verticillioides.

Mol Biol Rep 2019 Apr 20. Epub 2019 Apr 20.

Key Laboratory of Sugarcane Biotechnology and Genetic Improvement (Guangxi), Ministry of Agriculture, Sugarcane Research Institute, Guangxi Academy of Agricultural Sciences, Sugarcane Research Center, Chinese Academy of Agricultural Science, Nanning, 530007, China.

Fusarium verticillioides is the pathogen associated with pokkah boeng disease (PBD), the most significant airborne disease of sugarcane. The molecular mechanisms that regulate the defense responses of sugarcane towards this fungus are not yet fully known. Samples of 'YT 94/128' (resistant, R) and 'GT 37' (susceptible, S) inoculated with F. Read More

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http://link.springer.com/10.1007/s11033-019-04820-9
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http://dx.doi.org/10.1007/s11033-019-04820-9DOI Listing
April 2019
2 Reads

Yangyin Tongnao granules enhance neurogenesis in the peri-infarct area and upregulate brain-derived neurotrophic factor and vascular endothelial growth factor after focal cerebral ischemic infarction in rats.

Mol Biol Rep 2019 Apr 20. Epub 2019 Apr 20.

Zhejiang Chinese Medical University, Hangzhou, 310053, China.

Yangyin Tongnao granules (YYTNG) have been extensively applied in the treatment of brain injury, mainly due to its antioxidant effects, inhibition of apoptosis, and enhancement of blood circulation. To analyze the effect of YYTNG on the recovery of neurological function and neurogenesis in the peri-infarct area after cerebral ischemic infarction in rats and to elucidate its role in the neuroprotective mechanism of stroke, Sprague-Dawley (SD) rats were subjected to middle cerebral artery occlusion (MCAO) for 90 min followed by reperfusion. Rats were randomly divided into five groups: sham, MCAO, and YYTNG-treated rats given doses of 0. Read More

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http://dx.doi.org/10.1007/s11033-019-04824-5DOI Listing

The molecular basis and genotype-phenotype correlations of congenital adrenal hyperplasia (CAH) in Anatolian population.

Mol Biol Rep 2019 Apr 20. Epub 2019 Apr 20.

Department of Medical Genetics, Erciyes University, Kayseri, 38039, Turkey.

Congenital adrenal hyperplasia (CAH) is an autosomal recessive genetic disorder due to presence of mutations in the genes involved in the metabolism of steroid hormones in adrenal gland. There are two main forms of CAH, classic form and non-classic form. While classic form stands for the severe form, the non-classic form stands for the moderate and more frequent form of CAH. Read More

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http://link.springer.com/10.1007/s11033-019-04809-4
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http://dx.doi.org/10.1007/s11033-019-04809-4DOI Listing
April 2019
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Potential risk factors of ovarian cancer and analysis of CA125, a biomarker used for its monitoring and diagnosis.

Mol Biol Rep 2019 Apr 20. Epub 2019 Apr 20.

School of Life and Environmental Sciences, University of Sydney, Sydney, Australia.

Ovarian cancer (OC) is the fourth most common cancer among Pakistani, Scottish and Chinese women. The aim of the present study was to determine the association of potential risk factors with OC and analysis of Cancer Antigen 125 (CA125) in its monitoring and diagnosis. A total of 200 patients diagnosed with OC were included in this study. Read More

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http://dx.doi.org/10.1007/s11033-019-04794-8DOI Listing

Lycopene protects against t-BHP-induced neuronal oxidative damage and apoptosis via activation of the PI3K/Akt pathway.

Mol Biol Rep 2019 Apr 20. Epub 2019 Apr 20.

Department of Pathophysiology, Institute of Brain Science Research, Key Laboratory of State Administration of Traditional Chinese Medicine of the People's Republic of China, School of Medicine, Jinan University, Guangzhou, 510632, Guangdong, China.

Oxidative stress is a key factor of and closely implicated in the pathogenesis of Alzheimer's disease (AD). We herein used tert-butyl hydroperoxide (t-BHP) to induce oxidative stress and mimic oxidative neurotoxicity in vitro. Lycopene is a natural antioxidant that has a strong ability to eliminate free radicals and shows effective protection in some neurodegenerative disease models. Read More

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http://link.springer.com/10.1007/s11033-019-04801-y
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http://dx.doi.org/10.1007/s11033-019-04801-yDOI Listing
April 2019
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Characterization and molecular cloning of novel isoforms of human spermatogenesis associated gene SPATA3.

Mol Biol Rep 2019 Apr 20. Epub 2019 Apr 20.

Key Laboratory of Epigenetics and Oncology, the Research Center for Preclinical Medicine, Southwest Medical University, 3-319, Zhongshan Rd, Luzhou, 646000, Sichuan, China.

This study aimed to clone and characterize novel isoforms of the human SPATA3 gene. The isoforms of SPATA3 gene was cloned into pGMT vector using human testis cDNA as template, and Sanger sequencing was performed. Their characterizations and tissue-specific expression profiles were analyzed. Read More

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http://dx.doi.org/10.1007/s11033-019-04825-4DOI Listing

Effects of pomegranate aril juice and its punicalagin on some key regulators of insulin resistance and oxidative liver injury in streptozotocin-nicotinamide type 2 diabetic rats.

Mol Biol Rep 2019 Apr 20. Epub 2019 Apr 20.

Zoology Department, Faculty of Science, Ain Shams University, Khalifa El-Maamon St, Abbasiya Sq, Cairo, 11566, Egypt.

Nowadays, medicinal plants have been widely used everywhere to provide essential care for many disorders including diabetes. Recent reports assumed that the antidiabetic activities of pomegranate aril juice (PAJ) may be ascribed to its punicalagin (PCG). Therefore, the present study evaluated and compared the antidiabetic activities of PAJ and its PCG, and monitored some mechanisms of their actions in streptozotocin-nicotinamide (STZ-NA) type 2 diabetic rats. Read More

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http://dx.doi.org/10.1007/s11033-019-04813-8DOI Listing
April 2019
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M51R and Delta-M51 matrix protein of the vesicular stomatitis virus induce apoptosis in colorectal cancer cells.

Mol Biol Rep 2019 Apr 20. Epub 2019 Apr 20.

Cell and Molecular Research center, Golestan University of Medical Science, 1 Shastcola Ave, 5 km Sari Rd, Gorgan, Iran.

Colorectal cancer (CRC) is the third most common cancer in both men and women. Oncolytic viral-based therapy methods seem to be promising for CRC treatment. Vesicular stomatitis virus (VSV) is considered as a potent candidate in viral therapy for several tumors. Read More

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http://dx.doi.org/10.1007/s11033-019-04799-3DOI Listing

Potential role of lncRNA-TSIX, miR-548-a-3p, and SOGA1 mRNA in the diagnosis of hepatocellular carcinoma.

Mol Biol Rep 2019 Apr 19. Epub 2019 Apr 19.

Medical Biochemistry and Molecular Biology Department, Faculty of Medicine, Ain Shams University, Abbassia, Cairo, P.O. Box 11381, Egypt.

Recent trends are moving towards the use of the circulating transcriptome as a potential diagnostic and therapeutic tool for hepatocellular carcinoma (HCC). The aim of this study is to identify circulatory RNA based biomarker panel, in addition to their relationship to the outcome in HCC. First, utilizing bioinformatics tools, we selected an HCC-specific RNA-based biomarker panel that depended on the integration of suppressor of glucose autophagy-associated (SOGA1) gene expression with the chosen panel of epigenetic regulators of this gene [long non-coding RNA antisense for X-inactive-specific transcript (lncRNA-TSIX) and microRNA-548-a-3p (miR-548-a-3p)]. Read More

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http://link.springer.com/10.1007/s11033-019-04810-x
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http://dx.doi.org/10.1007/s11033-019-04810-xDOI Listing
April 2019
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Cytotoxicity screening of supercritical fluid extracted seaweeds and phenylpropanoids.

Mol Biol Rep 2019 Apr 19. Epub 2019 Apr 19.

Department of Bioengineering, Faculty of Engineering, Ege University, 35100, Bornova, Izmir, Turkey.

Detached leaves of Posidonia oceanica and Zostera marina creating nuisance at the shores were extracted by means of supercritical CO enriched with a co-solvent, compared with that of soxhlet extraction. The extracts and their active compounds which are phenylpropanoids (chicoric, p-coumaric, rosmarinic, benzoic, ferulic and caffeic acids) were screened for cytotoxicity in cancer cell lines including human breast adenocarcinoma (MCF-7, MDA-MB-231, SK-BR-3), human colon adenocarcinoma (HT-29), human cervix adenocarcinoma (HeLa), human prostate adenocarcinoma (PC-3), Mus musculus neuroblastoma (Neuro 2A) cell lines and African green monkey kidney (VERO) as healthy cell line. Supercritical CO extracts proved to be more active than soxhlet counterparts. Read More

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http://link.springer.com/10.1007/s11033-019-04812-9
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http://dx.doi.org/10.1007/s11033-019-04812-9DOI Listing
April 2019
1 Read

Blocking P2X7 receptor ameliorates oxidized LDL-mediated podocyte apoptosis.

Mol Biol Rep 2019 Apr 19. Epub 2019 Apr 19.

Department of Pediatric Nephrology and Rheumatism and Immunology, Shandong Provincial Hospital Affiliated to Shandong University, No. 324 Jing Wu Road, Jinan, 250021, People's Republic of China.

The purpose of our research is to elucidate whether oxLDL activates P2X7R in cultured human podocytes and if the activation of P2X7R leads to podocyte apoptosis. Additionally, we explore the underlying mechanism involved in podocyte apoptosis. Immortalized human podocytes were incubated with oxLDL (80 µg/ml), P2X7R antagonist A438079 (10 µM), or the compound of A438079 and oxLDL for 48 h, respectively. Read More

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http://dx.doi.org/10.1007/s11033-019-04823-6DOI Listing

New insight into poly (3-hydroxybutyrate) production by Azomonas macrocytogenes isolate KC685000: large scale production, kinetic modeling, recovery and characterization.

Mol Biol Rep 2019 Apr 17. Epub 2019 Apr 17.

Department of Microbiology and Immunology, Faculty of Pharmacy, Ain Shams University, Organization of African Unity St., Abbassia, 11566, Cairo, Egypt.

About 24 h incubation of Azomonas (A.) macrocytogenes isolate KC685000 in 14L fermenter produced 22% poly (3-hydroxybutyrate) (PHB) per cell dry weight (CDW) biopolymer using 1 vvm aeration, 10% inoculum size, and initial pH of 7.2. Read More

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http://dx.doi.org/10.1007/s11033-019-04798-4DOI Listing

Isolation and screening of extracellular anticancer enzymes from halophilic and halotolerant bacteria from different saline environments in Iran.

Mol Biol Rep 2019 Apr 16. Epub 2019 Apr 16.

Research Center for Prevention of Oral and Dental Diseases, Baqiyatallah University of Medical Sciences, Tehran, Iran.

It was confirmed that several enzymes have anti-cancer activity. The enzymes L-asparaginase, L-glutaminase, and L-arginase were chosen according to amino acids starvation in cancer cells and screened in halophilic and halotolerant bacteria, given probably less immunological reactions of halophilic or halotolerant enzymes in patients. Out of 110 halophilic and halotolerant strains, isolated from different saline environments in Iran and screened, some could produce a variety of anticancer enzymes. Read More

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http://dx.doi.org/10.1007/s11033-019-04787-7DOI Listing
April 2019
2.024 Impact Factor

Polyclonal predominance of concurrently producing OXA-23 and OXA-58 carbapenem-resistant Acinetobacter baumannii strains in a pediatric intensive care unit.

Mol Biol Rep 2019 Apr 12. Epub 2019 Apr 12.

Infectious Disease Unit, 3rd Department of Pediatrics, Medical Faculty, Aristotle University School of Health Sciences, Hippokration General Hospital, Konstantinoupoleos 49, 546 42, Thessaloniki, Greece.

We report a predominance (64.7%) of polyclonal carbapenem-resistant Acinetobacter baumannii (CRAB) strains concurrently producing OXA-23 and OXA-58 carbapenemases in a pediatric intensive care unit in an endemic area. This is the first report of emergence of such double-OXA CRAB strains in a single unit worldwide. Read More

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http://dx.doi.org/10.1007/s11033-019-04744-4DOI Listing
April 2019
2.024 Impact Factor

Haplotypes of CYP1B1 and CCDC57 genes in an Afro-Caribbean female population with uterine leiomyoma.

Mol Biol Rep 2019 Apr 13. Epub 2019 Apr 13.

Department of Biological and Chemical Sciences, Faculty of Science and Technology, University of the West Indies Cave Hill Campus, Bridgetown, Barbados.

Uterine leiomyomas (UL) are prevalent benign tumors, especially among women of African ancestry. The disease also has genetic liability and is influenced by risk factors such as hormones and obesity. This study investigates the haplotypes of the Cytochrome P450 1B1 gene (CYP1B1) related to hormones and coiled-coil domain containing 57 gene (CCDC57) related to obesity in Afro-Caribbean females. Read More

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http://link.springer.com/10.1007/s11033-019-04790-y
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http://dx.doi.org/10.1007/s11033-019-04790-yDOI Listing
April 2019
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The role of MAPK signaling pathway in formation of EMT in oral squamous carcinoma cells induced by TNF-α.

Mol Biol Rep 2019 Apr 15. Epub 2019 Apr 15.

Department of Stomatology, Yijishan Hospital of Wannan Medical College, Wuhu, 241002, People's Republic of China.

To study the role of MAPK signaling pathway in the development of Epithelial-mesenchymal transition in oral squamous cell carcinoma induced by inflammatory factor TNF-α. After the action of TNF-α, the expression of JNK, ERK, P38 in MAPK signaling pathway increased and the expression of E-cadherin, Claudin1 decreased significantly compared to the normal control group. After the addition of corresponding inhibitor, the expression of JNK, ERK, P38 decreased and the expression of E-cadherin, Claudin1 increased compared with TNF-α group. Read More

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http://link.springer.com/10.1007/s11033-019-04772-0
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http://dx.doi.org/10.1007/s11033-019-04772-0DOI Listing
April 2019
2 Reads

Is it useful to use several "omics" for obtaining valuable results?

Mol Biol Rep 2019 Apr 15. Epub 2019 Apr 15.

Departament of Biochemistry, Faculty of Veterinary Medicine, University of Life Science in Lublin, Akademicka Street 12, 20-033, Lublin, Poland.

The integration of cell communication and the transfer of signals from stimuli via transcription to translation and further to activation of new protein is crucial for appropriate metabolism and function of living organisms. The overall elucidation and the examination of these complex processes require multistep laboratory approaches in order to obtain results which will not only detect particular stage but also indicate the mechanisms lying upon this process. Such results will be reliable because they will cover multidirectional methods and approaches. Read More

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http://dx.doi.org/10.1007/s11033-019-04793-9DOI Listing

Identification and development of microsatellite (SSRs) makers of Exbucklandia (HAMAMELIDACEAE) by high-throughput sequencing.

Mol Biol Rep 2019 Apr 15. Epub 2019 Apr 15.

State Key Laboratory of Biocontrol and Guangdong Key Laboratory of Plant Resources, Sun Yat-sen University, Guangzhou, 510275, China.

Hamamelidaceae (Saxifragales, previously Rosales) comprises approximately six subfamily, 30 genera and 140 species, most of which are Tertiary relicts. Exbucklandia is the only genus of the subfamily Exbucklandioideae, Hamelidaceae, containing only 2-4 species. Of them, the species E. Read More

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http://dx.doi.org/10.1007/s11033-019-04800-zDOI Listing

Pro-atherogenic proteoglycanase ADAMTS-1 is down-regulated by lauric acid through PI3K and JNK signaling pathways in THP-1 derived macrophages.

Mol Biol Rep 2019 Apr 15. Epub 2019 Apr 15.

Department of Biomedical Science, Faculty of Science, Universiti Tunku Abdul Rahman, Jalan Universiti, Bandar Barat, 31900, Kampar, Perak, Malaysia.

The prevalence of atherosclerosis has increased significantly in the recent years due to sedentary lifestyle and high-fat diet. However, the association between saturated fat intake and the increased risk for atherosclerotic cardiovascular diseases remains heavily debated. Lauric acid belongs to the saturated fatty acid group and its unique medium chain fatty acid properties are proven to be beneficial to humans in many ways. Read More

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http://dx.doi.org/10.1007/s11033-019-04661-6DOI Listing

Identification of eleven different mutations including six novel, in the arylsulfatase B gene in Iranian patients with mucopolysaccharidosis type VI.

Mol Biol Rep 2019 Apr 13. Epub 2019 Apr 13.

Department of Medical Genetics and Molecular Biology, Iran University of Medical Sciences, Crossroads of Shahid Hemmat & Shahid Chamran Highways, P.O. Box 15875-6171, Tehran, 1449614535, Iran.

Mucopolysaccharidosis VI is a rare autosomal recessive disorder caused by the deficiency of enzyme Arylsulfatase B. The enzyme deficiency leads to the accumulation of dermatan sulfate in connective tissue which causes manifestations related to MPS VI. Up to now, three different disease causing variants are reported in Iranian patients. Read More

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http://dx.doi.org/10.1007/s11033-019-04804-9DOI Listing

Skeletal muscle proteome analysis provides insights on high altitude adaptation of yaks.

Mol Biol Rep 2019 Apr 13. Epub 2019 Apr 13.

Key Laboratory of Bio-resources and Eco-environment of the Ministry of Education, College of Life Sciences, Sichuan University, Chengdu, 610064, China.

The differences in proteome profile of longissimus thoracis (LT) muscles of yak (Bos grunniens) and cattle (Bos taurus) were investigated employing isobaric tag for relative and absolute quantification (iTRAQ) approach to identify differentially expressed proteins and to understand the cellular level adaptations of yaks to high altitudes. Fifty-two proteins were differentially expressed in the two species, among which 20 were up-regulated and 32 were down-regulated in yaks. Gene ontology (GO) annotation revealed that most of the differentially expressed proteins were involved in the molecular function of protein binding, catalytic activity, and structural activity. Read More

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http://dx.doi.org/10.1007/s11033-019-04732-8DOI Listing

In silico screening of sugar alcohol compounds to inhibit viral matrix protein VP40 of Ebola virus.

Mol Biol Rep 2019 Apr 13. Epub 2019 Apr 13.

School of Humanities, Nanyang Technological University, 14 Nanyang Dr, Singapore, 637332, Singapore.

Ebola virus is a virulent pathogen that causes highly lethal hemorrhagic fever in human and non-human species. The rapid growth of this virus infection has made the scenario increasingly complicated to control the disease. Receptor viral matrix protein (VP40) is highly responsible for the replication and budding of progeny virus. Read More

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http://dx.doi.org/10.1007/s11033-019-04792-wDOI Listing

In-silico analysis of novel p.(Gly14Ser) variant of ATOX1 gene: plausible role in modulating ATOX1-ATP7B interaction.

Mol Biol Rep 2019 Apr 12. Epub 2019 Apr 12.

Department of Biochemistry, PGIMER, Chandigarh, India.

Clinical heterogeneity is commonly observed in Wilson disease (WD), including cases with identical ATP7B mutations. It is thought to be an outcome of impairment in other genes involved in cellular copper homeostasis in addition to the mutations in the ATP7B gene. ATOX1, a copper chaperone that delivers copper to ATP7B, is a potential genetic modifier of WD. Read More

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http://link.springer.com/10.1007/s11033-019-04791-x
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http://dx.doi.org/10.1007/s11033-019-04791-xDOI Listing
April 2019
2 Reads

PYHIN genes as potential biomarkers for prognosis of human papillomavirus-positive or -negative head and neck squamous cell carcinomas.

Mol Biol Rep 2019 Apr 12. Epub 2019 Apr 12.

Department of Public Health and Pediatric Sciences, University of Turin, Via Santena 9, 10126, Turin, Italy.

The aim of the present study is to determine the expression levels of PYHIN (IFI16 and AIM2) and APOBEC3 (A3A, A3B, A3C, A3D, A3F, A3G, and A3H) gene family members in a cohort of patients with head and neck squamous cell carcinoma (HNSCC) and assess their potential correlation with human papillomavirus (HPV) infection status, clinical characteristics, and survival. For this purpose, 34 HNSCC tissue specimens along with healthy surrounding mucosa were collected from patients surgically treated for HNSCC. Nucleic acids were isolated to assess the presence of HPV and the expression levels of selected molecular markers. Read More

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http://dx.doi.org/10.1007/s11033-019-04795-7DOI Listing
April 2019
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Marine macroalga Caulerpa: role of its metabolites in modulating cancer signaling.

Mol Biol Rep 2019 Apr 12. Epub 2019 Apr 12.

Laboratory of Molecular Medicine, Department of Human Genetics and Molecular Medicine, Central University of Punjab, Mansa Road, Bathinda, Punjab, 151001, India.

Cancer, the leading causes of death worldwide, causes multiple metabolic and physiological alterations, leading to an unregulated proliferation of cells. The existing anticancer therapies are usually nonspecific with side effects and or are extremely expensive, thus hunt for better therapeutics is still on, specially efforts are made to look for naturally occurring molecules. Sea harbors several organisms which are unexplored for their biological potentials. Read More

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http://link.springer.com/10.1007/s11033-019-04743-5
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http://dx.doi.org/10.1007/s11033-019-04743-5DOI Listing
April 2019
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Cyanobacterial bioactive compound EMTAHDCA recovers splenomegaly, affects protein profile of E. coli and spleen of lymphoma bearing mice.

Mol Biol Rep 2019 Apr 12. Epub 2019 Apr 12.

Laboratory of Microbial Genetics, Department of Botany, Banaras Hindu University, Varanasi, 221005, India.

The antibacterial and anticancerous properties of EMTAHDCA have already been reported in our previous study. However, mode of action of EMTAHDCA is still elusive. The present study was aimed to investigate the molecular targets in Escherichia coli and spleen of lymphoma-bearing mice in response to cyanocompound 9-ethyliminomethyl-12 (morpholin-4-ylmethoxy)-5, 8, 13, 16-tetraaza -hexacene-2, 3- dicarboxylic acid (EMTAHDCA) isolated from fresh water cyanobacterium Nostoc sp. Read More

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http://link.springer.com/10.1007/s11033-019-04659-0
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http://dx.doi.org/10.1007/s11033-019-04659-0DOI Listing
April 2019
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The newly developed single nucleotide polymorphism (SNP) markers for a potentially medicinal plant, Crepidiastrum denticulatum (Asteraceae), inferred from complete chloroplast genome data.

Mol Biol Rep 2019 Apr 12. Epub 2019 Apr 12.

Department of Life Science, Gachon University, Seongnam, 13120, Republic of Korea.

Medicinal effects of Crepidiastrum denticulatum have been previously reported. However, the genomic resources of this species and its applications have not been studied. In this study, based on the next generation sequencing method (Miseq sequencing system), we characterize the chloroplast genome of C. Read More

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http://dx.doi.org/10.1007/s11033-019-04789-5DOI Listing
April 2019
2.024 Impact Factor

Production optimization, characterization, and covalent immobilization of a thermophilic Serratia rubidaea lipase isolated from an Algerian oil waste.

Mol Biol Rep 2019 Apr 12. Epub 2019 Apr 12.

Laboratory of Microbial Biotechnology and Engineering Enzymes (LMBEE), Centre of Biotechnology of Sfax (CBS), University of Sfax, Sidi Mansour Road Km 6, P.O. Box 1177, Sfax, 3018, Tunisia.

A new thermophilic non-induced lipase producer named Serratia rubidaea strain Nehal-mou was isolated from oil waste in Tissemsilat, Algeria. The most influential lipase production parameters were screened by the Plackett-Burman design for enhancing enzyme yield. An optimum condition of a 1. Read More

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http://dx.doi.org/10.1007/s11033-019-04774-yDOI Listing

Female gender specific association of the Reelin (RELN) gene rs7341475 variant with schizophrenia.

Mol Biol Rep 2019 Apr 12. Epub 2019 Apr 12.

Department of Psychiatry, Faculty of Medicine, University of Kocaeli, Kocaeli, Turkey.

RELN gene encodes a large extracellular matrix protein which is critical for neuronal migration, cell positioning and cell-cell interactions. It also controls the synaptic plasticity of neurons for initiation and maintenance of long term potentiation. The aim of this study is to investigate the association of RELN rs7341475 variant with schizophrenia. Read More

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http://dx.doi.org/10.1007/s11033-019-04803-wDOI Listing

Analysis of differential gene expression of the transgenic pig with overexpression of PGC1α in muscle.

Mol Biol Rep 2019 Apr 12. Epub 2019 Apr 12.

Key Lab of Swine Genetics and Breeding of Ministry of Agriculture and Rural Affairs and Key Lab of Agricultural Animal Genetics and Breeding, Ministry of Education, College of Animal Science and Veterinary Medicine, Huazhong Agricultural University, Wuhan, 430070, People's Republic of China.

In order to better understand the key regulatory mechanisms of PGC1α in muscle fiber type transition, the RNA-seq was used to compare the change of gene expression in gastrocnemius muscles between wild type pigs and transgenic pigs with overexpression of PGC1α gene in muscle. 371 differentially expressed genes (P ≤ 0.05 and Ratio ≥ 2), including 184 up-regulated genes and 187 down-regulated genes, were identified. Read More

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http://dx.doi.org/10.1007/s11033-019-04805-8DOI Listing

Efficient episomal gene transfer to human hepatic cells using the pFAR4-S/MAR vector.

Mol Biol Rep 2019 Apr 12. Epub 2019 Apr 12.

Department of General Biology, Medical School, University of Patras, 26504, Rion, Patras, Greece.

Liver-directed gene therapy, using mainly viral vectors for the genetic cell modification, is a promising therapeutic approach for many genetic and metabolic liver diseases. The recent successful preclinical trials with AAV vectors expose the benefits as well as the limitations of the system. We focused on the development of an alternative non-viral episomal gene transfer system, by inserting the DNA element Scaffold/Matrix Attachment Region (S/MAR) into the free of antibiotic resistance gene miniplasmid vector (pFAR4). Read More

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http://link.springer.com/10.1007/s11033-019-04777-9
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http://dx.doi.org/10.1007/s11033-019-04777-9DOI Listing
April 2019
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Strong association between VDR FokI (rs2228570) gene variant and serum vitamin D levels in Turkish Cypriots.

Mol Biol Rep 2019 Apr 12. Epub 2019 Apr 12.

Department of Medical Biology, Faculty of Medicine, Near East University, 99138, Nicosia, Cyprus.

Vitamin D is an important molecule to keep teeth, bones and muscles healthy. It is obtained from diet, supplements and primarily from exposure to sunlight. In recent years, vitamin D deficiency is recognised as a worldwide health problem, which results in disturbances in mineral metabolism and skeletal problems. Read More

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http://dx.doi.org/10.1007/s11033-019-04796-6DOI Listing

Protective properties of the cultured stem cell proteome studied in an animal model of acetaminophen-induced acute liver failure.

Mol Biol Rep 2019 Apr 12. Epub 2019 Apr 12.

Institute of Science and Technology (IST Austria), Klosterneuburg, Austria.

Chronic overuse of common pharmaceuticals, e.g. acetaminophen (paracetamol), often leads to the development of acute liver failure (ALF). Read More

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http://link.springer.com/10.1007/s11033-019-04765-z
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http://dx.doi.org/10.1007/s11033-019-04765-zDOI Listing
April 2019
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Blood pressure lowering effect of Ficus deltoidea var kunstleri in spontaneously hypertensive rats: possible involvement of renin-angiotensin-aldosterone system, endothelial function and anti-oxidant system.

Mol Biol Rep 2019 Apr 12. Epub 2019 Apr 12.

Faculty of Medicine, Universiti Teknologi MARA, Sungai Buloh Campus, Sungai Buloh, Selangor, Malaysia.

This study investigated the effects of a standardised ethanol and water extract of Ficus deltoidea var. Kunstleri (FDK) on blood pressure, renin-angiotensin-aldosterone system (RAAS), endothelial function and antioxidant system in spontaneously hypertensive rats (SHR). Seven groups of male SHR were administered orally in volumes of 0. Read More

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http://dx.doi.org/10.1007/s11033-019-04730-wDOI Listing
April 2019
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Adaptation of Pseudomonas aeruginosa clinical isolates to benzalkonium chloride retards its growth and enhances biofilm production.

Mol Biol Rep 2019 Apr 11. Epub 2019 Apr 11.

Department of Pharmaceutical Microbiology, Faculty of Pharmacy, Medical Campus, Tanta University, El-Geish Street, Tanta, El-Gharbia, 31611, Egypt.

The increasing percentage of Pseudomonas aeruginosa strains that are resistant to multiple antibiotics is a global problem. The exposure of P. aeruginosa isolates to repeated sub lethal concentrations of biocides in hospitals and communities may be one of the causes leading to increased antibiotic resistance. Read More

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http://dx.doi.org/10.1007/s11033-019-04806-7DOI Listing

Molecular cloning and expression analysis of SRLK1 gene in self-incompatible Asteraceae species Erigeron breviscapus.

Mol Biol Rep 2019 Apr 8. Epub 2019 Apr 8.

National and Local Joint Engineering Research Center on Germplasm Utilization & Innovation of Chinese Medicinal Materials in Southwest, Yunnan Agricultural University, Kunming, 650201, Yunnan, China.

Based on the transcriptome data, using RACE techniques, we cloned the full-length EbSRLK1 gene in a medicinal, self-incompatible Asteraceae species, Erigeron breviscapus. Bioinformatics approaches were used to analyze the DNA and protein sequences, physical and chemical properties, and domains of the encoded protein. The full-length EbSRLK1 cDNA is 2891 base pairs (bp) with an open reading frame (ORF) of 2634 bp, which encodes the EbSRLK1 protein with 878 amino acids and an estimated molecular weight of 98. Read More

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http://dx.doi.org/10.1007/s11033-019-04773-zDOI Listing
April 2019
2.024 Impact Factor

Quantitative expression of microRNAs in Brassica oleracea infected with Xanthomonas campestris pv. campestris.

Mol Biol Rep 2019 Apr 3. Epub 2019 Apr 3.

Embrapa Recursos Genéticos e Biotecnologia, Parque Estação Biológica, PqEB, Av. W5, Norte (final), Brasília, DF, CEP 70770-917, Brazil.

Brassica oleracea var. capitata (cabbage) is an economically important crop affected by black rot disease caused by Xanthomonas campestris pv. campestris (Xcc). Read More

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http://dx.doi.org/10.1007/s11033-019-04779-7DOI Listing
April 2019
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Phytochemical, antioxidant, anti-proliferative and antimicrobial properties of Catharanthus roseus root extract, saponin-enriched and aqueous fractions.

Mol Biol Rep 2019 Apr 3. Epub 2019 Apr 3.

School of Environmental and Life Sciences, Faculty of Science, University of Newcastle, Brush Road, Ourimbah, NSW, 2258, Australia.

Catharanthus roseus (L.) G. Don (C. Read More

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http://dx.doi.org/10.1007/s11033-019-04786-8DOI Listing
April 2019
2 Reads