6,755 results match your criteria Molecular Biology Reports [Journal]


Genetic relationships among subspecies of Capparis spinosa L. from Tunisia by using ISSR markers.

Mol Biol Rep 2019 Feb 15. Epub 2019 Feb 15.

Department of Biology, Laboratory of Plant Biotechnology, Centre Urbain Nord, National Institute of Applied Sciences and Technologies (INSAT), B.P. 676, 1080, Tunis Cedex, Tunisia.

In Tunisia, Capparis spinosa L. is widely distributed in different geographic areas. Although it has been extensively studied morphologically, the phylogenetic relationships by using molecular markers among Capparis taxa are still unclear. Read More

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http://dx.doi.org/10.1007/s11033-019-04676-zDOI Listing
February 2019

Phytosterols and triterpenes from Morinda lucida Benth. exhibit binding tendency against class I HDAC and HDAC7 isoforms.

Mol Biol Rep 2019 Feb 15. Epub 2019 Feb 15.

Biochemistry Unit, Department of Chemical Sciences, Faculty of Natural Sciences, Ajayi Crowther University Oyo, Oyo, Oyo State, Nigeria.

The important role of histone deacetylases (HDACs) in the development of cancer has been demonstrated by various studies. Thus targeting HDACs with inhibitors is a major focus in anticancer drug research. Although few synthetic HDAC inhibitors (HDIs) have been approved for cancer treatment, they have significant undesirable side effects. Read More

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http://dx.doi.org/10.1007/s11033-019-04689-8DOI Listing
February 2019

Complete mitochondrial genome of Indian mithun, Bos frontalis and its phylogenetic implications.

Mol Biol Rep 2019 Feb 14. Epub 2019 Feb 14.

Department of Genomic Science, School of Biological Sciences, Central University of Kerala, Kasaragod, Kerala, 671316, India.

Mithun (Bos frontalis) is an endangered domestic bovine species native to the hilly areas of China, Bangladesh, Myanmar, Bhutan and India. It is believed to have been domesticated from gaur around 8000 years ago. However, a few studies suggest that mithun is either an independent species or a hybrid descendant of gaur and cattle. Read More

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http://link.springer.com/10.1007/s11033-019-04675-0
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http://dx.doi.org/10.1007/s11033-019-04675-0DOI Listing
February 2019
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In vitro antioxidant assessment, screening of enzyme inhibitory activities of methanol and water extracts and gene expression in Hypericum lydium.

Mol Biol Rep 2019 Feb 14. Epub 2019 Feb 14.

Agronomy and Plant Breeding Department, Razi University, Kermanshah, Iran.

Hypericum lydium Boiss. is a perennial plant of the Hypericaceae family, which has been used in particular to treat depression. The aim of this study was to determine in vitro antioxidant, antimicrobial activities, anticholinesterase (acetylcholinesterase (AChE)/butyrylcholinesterase (BChE)), antidiabetic activities (α-glucosidase/α-amylase) and Tyrosinase inhibitor activity of methanol and water extracts of H. Read More

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http://dx.doi.org/10.1007/s11033-019-04664-3DOI Listing
February 2019

Comparative RNA editing profile of mitochondrial transcripts in cytoplasmic male sterile and fertile pigeonpea reveal significant changes at the protein level.

Mol Biol Rep 2019 Feb 13. Epub 2019 Feb 13.

ICAR-National Research Centre on Plant Biotechnology, New Delhi, 110012, India.

RNA editing is a process which leads to post-transcriptional alteration of the nucleotide sequence of the corresponding mRNA molecule which may or may not lead to changes at the protein level. Apart from its role in providing variability at the transcript and protein levels, sometimes, such changes may lead to abnormal expression of the mitochondrial gene leading to a cytoplasmic male sterile phenotype. Here we report the editing status of 20 major mitochondrial transcripts in both male sterile (AKCMS11) and male fertile (AKPR303) pigeonpea genotypes. Read More

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http://dx.doi.org/10.1007/s11033-019-04657-2DOI Listing
February 2019

Renal effects of exendin-4 in an animal model of brain death.

Mol Biol Rep 2019 Feb 13. Epub 2019 Feb 13.

Laboratory of Human Pancreatic Islet Biology, Endocrine Division, Hospital de Clínicas de Porto Alegre, Rua Ramiro Barcelos 2350, prédio 12, 4° andar, Porto Alegre, Rio Grande Do Sul, 90035-003, Brazil.

Organ transplantation is the gold standard therapy for the majority of patients with terminal organ failure. However, it is still a limited treatment especially due to the low number of brain death (BD) donors in relation to the number of waiting list recipients. Strategies to increase the quantity and quality of donor organs have been studied, and the administration of exendin-4 (Ex-4) to the donor may be a promising approach. Read More

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http://dx.doi.org/10.1007/s11033-019-04674-1DOI Listing
February 2019

New insights into the catalytic inactivity of mammary gland protein-40, a chitinase-like protein expressed during mammary gland involution.

Mol Biol Rep 2019 Feb 13. Epub 2019 Feb 13.

Proteomics and Structural Biology Lab, Animal Biotechnology Center, National Dairy Research Institute, 132001, Karnal, India.

MGP-40 is a mammary gland-specific glycoprotein which is expressed during involution and is an important marker for mammary gland apoptosis. It is an inactive chitinase-like protein belonging to Glycosyl Hydrolase family 18. The present study reports sequence characterization, tissue-specific expression analysis, production of recombinant MGP-40 and its mutant (A117D and L119E) in both E. Read More

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http://dx.doi.org/10.1007/s11033-019-04679-wDOI Listing
February 2019

Can wheat survive in heat? Assembling tools towards successful development of heat stress tolerance in Triticum aestivum L.

Mol Biol Rep 2019 Feb 13. Epub 2019 Feb 13.

Plant Tissue Culture and Genetic Engineering, National Agri-Food Biotechnology Institute (NABI), Sector-81, S.A.S. Nagar, Mohali, Punjab, 140306, India.

Wheat is an important cereal crop that fulfils the calorie demands of the global humanity. Rapidly expanding populations are exposed to a fast approaching acute shortage in the adequate supply of food and fibre from agricultural resources. One of the significant threats to food security lies in the constantly increasing global temperatures which inflict serious injuries to the plants in terms of various physiological, biochemical and molecular processes. Read More

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http://dx.doi.org/10.1007/s11033-019-04686-xDOI Listing
February 2019

High levels of heavy metals in Western Arabian Gulf mangrove soils.

Mol Biol Rep 2019 Feb 13. Epub 2019 Feb 13.

Department of Biology, College of Science, Imam Abdulrahman Bin Faisal University (IAU), Dammam, 31441-1982, Saudi Arabia.

Major development along the Western Arabian Gulf coast has disturbed the marine environment, and led to increased concentrations of heavy metals in the coastal soils. The amount of 13 of these metals (Ag, Al, As, Cd, Cr, Cu, Fe, Mn, Mo, Ni, Pb, V and Zn) in Avicennia marina branches and leaves as well as in rhizosphere soil samples from two Bays 70 km apart (Tarut Bay; Saudi Arabia and Tubli Bay; Bahrain) was quantified. Heavy metal concentration in the two bays were similar and higher than those reported in other regions suggesting a generalized heavy metal pollution in the area. Read More

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http://dx.doi.org/10.1007/s11033-019-04603-2DOI Listing
February 2019

Phenolic compounds, antioxidant capacity and antimutagenic activity in different growth stages of in vitro raised plants of Origanum vulgare L.

Mol Biol Rep 2019 Feb 12. Epub 2019 Feb 12.

G. B. Pant National Institute of Himalayan Environment and Sustainable Development, Kosi-Katarmal, Almora, Uttarakhand, 263643, India.

Efficient micropropagation procedure was developed for Origanum vulgare, a high-value culinary herb, and the phytochemicals, phenolic content, antioxidant and antimutagenic activity of leaf and stem, derived from different growing stages were analyzed. The agar solidified Murashige and Skoog (MS) medium supplemented with a combination of 6-benzylaminopurine and α-naphthaleneacetic acid was optimized as best shoot-multiplication-medium. Shoots were rooted best on 1/2 strength MS medium supplemented with 50 µM indole-3-butyric acid (IBA). Read More

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http://dx.doi.org/10.1007/s11033-019-04678-xDOI Listing
February 2019

Isoflavones prevent oxidative stress and inhibit the activity of the enzyme monoamine oxidase in vitro.

Mol Biol Rep 2019 Feb 12. Epub 2019 Feb 12.

Mestrado em Ciências da Saúde e da Vida, Universidade Franciscana (UFN), Santa Maria, Rio Grande do Sul, 97010-032, Brazil.

Oxidative stress occurs due to an imbalance between antioxidant defenses and pro-oxidant agents in brain. This condition has been associated to the pathogenesis of several brain diseases; therefore, increasing the use of compounds that exert antioxidant activity. Thus, the objective of this study was to evaluate, in vitro, the effect of isoflavones in: (1) lipid peroxidation, catalase activity and thiol groups in the presence of pro-oxidants: sodium nitroprusside or Fe/EDTA complex in rat brain homogenates; (2) the activity of the enzyme monoamine oxidase (MAO). Read More

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http://dx.doi.org/10.1007/s11033-019-04684-zDOI Listing
February 2019

Genome-wide screening of hexokinase gene family and functional elucidation of HXK2 response to cold stress in Jatropha curcas.

Mol Biol Rep 2019 Feb 12. Epub 2019 Feb 12.

Center for Yunnan Plateau Biological Resources Protection and Utilization, Qujing Normal University, Qujing, 655011, Yunnan, China.

Hexokinase, the key rate-limiting enzyme of plant respiration and glycolysis metabolism, has been found to play a vital role in plant sugar sensing and sugar signal transduction. Using Jatropha curcas genome database and bioinformatics method, J. curcas HXK gene family (JcHXK) was identified and its phylogenetic evolution, functional domain, signal peptide at the N-terminal, and expression analysis were conducted. Read More

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http://link.springer.com/10.1007/s11033-019-04613-0
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http://dx.doi.org/10.1007/s11033-019-04613-0DOI Listing
February 2019
1 Read

Sirt1 antisense transcript is down-regulated in human tumors.

Mol Biol Rep 2019 Feb 12. Epub 2019 Feb 12.

Department of Biotechnology, School of Advanced Technologies in Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.

Natural antisense transcripts (NATs) have recently been associated with the development of human cancers. Recent studies have shown that a natural antisense transcript (NAT) is present in Sirt1 gene which encodes a NAD-dependent deacetylase. Interestingly, expression of Sirt1 mRNA changes during development and progression of human cancers. Read More

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http://dx.doi.org/10.1007/s11033-019-04687-wDOI Listing
February 2019
2.024 Impact Factor

Calendula arvensis L. as an anti-cancer agent against breast cancer cell lines.

Mol Biol Rep 2019 Feb 11. Epub 2019 Feb 11.

Bioproducts Research Chair, Department of Zoology, College of Science, King Saud University, Riyadh, Saudi Arabia.

Calendula arvensis L. is used in traditional folk medicine for the treatment of several diseases. Leaves, stems, and flowers of C. Read More

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http://dx.doi.org/10.1007/s11033-019-04672-3DOI Listing
February 2019

A novel myelin basic protein transcript variant in the murine central nervous system.

Mol Biol Rep 2019 Feb 12. Epub 2019 Feb 12.

Bascom Palmer Eye Institute, University of Miami, Miami, FL, USA.

Myelin basic protein is a multifunctional protein whose primary role is to adhere membranes of the myelin sheath. There are various isoforms that have been identified, 6 distinct isoforms in human and 13 distinct isoforms in mice. These distinct isoforms are the product of alternative splicing of a single gene. Read More

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http://dx.doi.org/10.1007/s11033-019-04635-8DOI Listing
February 2019

Decreased MMP1 gene expression in acute myeloid leukaemia.

Mol Biol Rep 2019 Feb 12. Epub 2019 Feb 12.

Laboratory of Molecular Diagnostics and Pharmacogenomics, Department of Pharmaceutical Biochemistry and Molecular Diagnostics, Medical University of Lodz, Muszynskiego 1, 90-151, Lodz, Poland.

Acute myeloid leukaemia (AML) is a heterogeneous disorder of haematopoietic stem cells or progenitor cells. Metalloproteinases (MMPs) are proteolytic enzymes whose activity is increased in different types of solid tumours. These enzymes regulate many processes associated with tumour progression. Read More

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http://dx.doi.org/10.1007/s11033-019-04685-yDOI Listing
February 2019

Fractionated whole body gamma irradiation modulates the hepatic response in type II diabetes of high fat diet model rats.

Mol Biol Rep 2019 Feb 12. Epub 2019 Feb 12.

Department of Molecular Biology and Biotechnology, AECS, P.O. Box 609, Damascus, Syria.

HFD animals were exposed to a low rate of different fractionated whole body gamma irradiation doses (0.5, 1 and 2 Gy, three fractions per week for two consecutive months) and the expression of certain genes involved in type 2 diabetes mellitus (T2DM) in livers and brains of HFD Wistar rats was investigated. Additionally, levels of diabetes-related proteins encoded by the studied genes were analyzed. Read More

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http://dx.doi.org/10.1007/s11033-019-04681-2DOI Listing
February 2019
1 Read
2.024 Impact Factor

Effects of dietary Allium mongolicum Regel polysaccharide on growth, lipopolysaccharide-induced antioxidant responses and immune responses in Channa argus.

Mol Biol Rep 2019 Feb 12. Epub 2019 Feb 12.

College of Animal Science and Technology, Jilin Agricultural University, Changchun, 130118, Jilin, China.

The present study evaluated the effects of dietary Allium mongolicum Regel polysaccharide (AMRP) on growth, lipopolysaccharide-induced antioxidant responses and immune responses in Channa argus. A basal diet was supplemented with AMRP at 0, 1, 1.5 or 2 g/kg feed for 56 days. Read More

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http://dx.doi.org/10.1007/s11033-019-04677-yDOI Listing
February 2019
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Baicalin induced colon cancer cells apoptosis through miR-217/DKK1-mediated inhibition of Wnt signaling pathway.

Mol Biol Rep 2019 Feb 8. Epub 2019 Feb 8.

, Taiyuan, China.

To analyze the anti-tumor mechanism of Baicalin in human colon cancer. The MTT assay and colony formation assay demonstrated that Baicalin treatment inhibits the proliferation of DLD1 and HCT-116 cells. The apoptosis rates were induced upon Baicalin treatment and which was determined by FACS. Read More

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http://link.springer.com/10.1007/s11033-019-04618-9
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http://dx.doi.org/10.1007/s11033-019-04618-9DOI Listing
February 2019
3 Reads

Epistasis between ADIPOQ rs1501299 and PON1 rs662 polymorphisms is potentially associated with the development of knee osteoarthritis.

Mol Biol Rep 2019 Feb 8. Epub 2019 Feb 8.

Rheumatology Department, Instituto Nacional de Rehabilitación "Luis Guillermo Ibarra Ibarra", Calzada Mexico-Xochimilco 289, Col. Arenal de Guadalupe, Tlalpan, 14389, Mexico City, Mexico.

Overweight produces oxidative stress (OS) on the articular cartilage, with the subsequent risk of developing knee osteoarthritis (OA). Associations between genetic polymorphisms related to OS and OA have been reported, but it is currently unknown whether there exist interactions among them that affect OA development. To identify and evaluate interactions between multiple SNPs related to OS in Mexican knee OA patients. Read More

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http://dx.doi.org/10.1007/s11033-019-04654-5DOI Listing
February 2019
1 Read

Family-1 UDP glycosyltransferases in pear (Pyrus bretschneideri): Molecular identification, phylogenomic characterization and expression profiling during stone cell formation.

Mol Biol Rep 2019 Feb 7. Epub 2019 Feb 7.

School of Life Science, Anhui Agricultural University, No. 130, Changjiang West Road, Hefei, 230036, China.

Stone cells are a characteristic trait of pear fruits, and excessive stone cell formation has a significant negative impact on the texture and flavour of the pulp. Lignin is one of the main components of stone cells. Family-1 uridine diphosphate-glycosyltransferases (UGTs) are responsible for the glycosylation modification of monolignols. Read More

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http://dx.doi.org/10.1007/s11033-019-04669-yDOI Listing
February 2019

Exposure to bisphenol A: current levels from food intake are toxic to human cells.

Mol Biol Rep 2019 Feb 7. Epub 2019 Feb 7.

Departamento de Alimentos y Biotecnologia, Facultad de Quimica, Universidad Nacional Autónoma de Mexico, Ciudad Universitaria, CP 04510, Coyoacan, Mexico.

In the present work, cell lines of different origin were exposed to BPA levels from food intake reported elsewhere. Specifically, we used an in vitro assay to determine cytotoxicity of BPA in three cell lines: MCF7 (breast cancer), PC3 (prostate cancer) and 3T3-L1 (mouse fibroblast). Cytotoxic effects were observed at concentrations higher than 50 μg/mL which is above the involuntary exposure level of BPA described before in fresh, canned and frozen foods and beverages. Read More

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http://dx.doi.org/10.1007/s11033-019-04666-1DOI Listing
February 2019

Validation of a one-step PCR assay for the molecular identification of Echinococcus granulosus sensu stricto G1-G3 genotype.

Mol Biol Rep 2019 Feb 7. Epub 2019 Feb 7.

OIE and National Reference Laboratory for Echinococcosis (CeNRE), Istituto Zooprofilattico Sperimentale della Sardegna, Sassari, Italy.

The Italian National Reference Center for Echinococcosis (CeNRE, Sassari, Italy) set up a diagnostic protocol of "one-step-PCR" useful for the detection of E. granulosus sensu stricto (E.g. Read More

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http://dx.doi.org/10.1007/s11033-019-04625-wDOI Listing
February 2019
1 Read

Effect of selenium on growth and antioxidative system of yeast cells.

Mol Biol Rep 2019 Feb 7. Epub 2019 Feb 7.

Faculty of Food Sciences, Department of Biotechnology, Microbiology and Food Evaluation, Warsaw University of Life Sciences-SGGW, Nowoursynowska 159 C, 02-776, Warsaw, Poland.

Selenium exhibits health-promoting properties in humans and animals. Therefore, the development of selenium-enriched dietary supplements has been growing worldwide. However, it may also exhibit toxicity at higher concentrations, causing increased oxidative stress. Read More

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http://dx.doi.org/10.1007/s11033-019-04630-zDOI Listing
February 2019

Analysis of p.Gly12Valfs*2, p.Trp24* and p.Trp77Arg mutations in GJB2 and p.Arg81Gln variant in LRTOMT among non syndromic hearing loss Egyptian patients: implications for genetic diagnosis.

Mol Biol Rep 2019 Feb 7. Epub 2019 Feb 7.

Laboboratoire Procédés de Criblage Moléculaire et Cellulaire, Centre de Biotechnologie de Sfax, Université de Sfax, Sfax, Tunisia.

Hearing loss (HL) is a global sensory disorder that affects children and deprives them from their rights to enjoy standard social and educational levels. Although hundreds of genetic mutations across several genes have been linked to HL, very limited studies are available on Egyptian population which has high rate of consanguinity and HL. The frequency of the p. Read More

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http://dx.doi.org/10.1007/s11033-019-04667-0DOI Listing
February 2019

Myxococcus xanthus truncated globin HbO: in silico analysis and functional characterization.

Mol Biol Rep 2019 Feb 7. Epub 2019 Feb 7.

Department cum National Centre for Human Genome Studies and Research, Panjab University, Chandigarh, India.

Truncated globins are 20-40 amino acids shorter than full length globins. Till date, globins have been characterized predominantly from bacteria involved in pathogenicity, nitrogen fixation and photosynthesis, where they are implicated in bacterial virulence within the host, protection of nitrogenase from oxygen inactivation and prevention of oxidative damage to the photosynthetic machinery respectively. Myxococcus xanthus, the model myxobacterium, is an obligate aerobe with a multicellular stage in its life cycle where cells encounter oxygen limitation. Read More

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http://dx.doi.org/10.1007/s11033-019-04662-5DOI Listing
February 2019
1 Read
2.024 Impact Factor

High fat diet administration leads to the mitochondrial dysfunction and selectively alters the expression of class 1 GLUT protein in mice.

Mol Biol Rep 2019 Feb 6. Epub 2019 Feb 6.

Department of Pharmaceutical Sciences and Technology, Birla Institute of Technology, Mesra, Ranchi, Jharkhand, 835215, India.

Metabolic syndrome is an agglomeration of disorders including obesity, diabetes and cardiovascular diseases and characterized as chronic mild inflammation which elevates the circulatory inflammatory markers. This could be due to mitochondrial dysfunction, oxidative stress and hypoxia as a consequence of high fat diet (HFD) intake. The present study focuses on the effects of HFD on lactate and mitochondrial metabolism as well as tissue dependent changes in glucose transporter (GLUT) expression in liver, skeletal muscles and adipose tissue of mouse. Read More

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http://dx.doi.org/10.1007/s11033-019-04623-yDOI Listing
February 2019
1 Read

Combining bioinformatics and conventional PCR optimization strategy for one-time design of high-specificity primers for WRKY gene family using unigene database.

Mol Biol Rep 2019 Feb 6. Epub 2019 Feb 6.

Plant Cell Biotechnology Department, CSIR-Central Food Technological Research Institute, KRS Road, Mysuru, Karnataka, 570020, India.

Gene families, like the conserved transcription factor families, evolve through gene duplications and share moderate similarity between member genes. Lack of genomic data makes it difficult to design high-specificity primers to the target genes. Furthermore, many primers under-perform in highly sensitive assays like quantitative PCR due to issues of thermodynamic nature, thereby increasing the cost and time for analysis. Read More

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http://link.springer.com/10.1007/s11033-018-04577-7
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http://dx.doi.org/10.1007/s11033-018-04577-7DOI Listing
February 2019
4 Reads

The anti-cancer effect of amygdalin on human cancer cell lines.

Mol Biol Rep 2019 Feb 5. Epub 2019 Feb 5.

Young Researchers and Elite Club, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran.

Derived from rosaceous plant seed, amygdalin belongs to aromatic cyanogenic glycoside group, and its anticancer effects have been supported by mounting evidence. In this study, we objected to investigate amygdalin effect on two antiapoptotic genes (Survivin, XIAP) and two lncRNAs (GAS5, MALAT1) in human cancer cells (A549, MCF7, AGS). Employing RT-qPCR analysis, we compared the mRNA levels of the genes related to apoptosis in A549, MCF7, and AGS cancer cells between amygdalin-treated (24, 48 and 72 h) and un-treated groups. Read More

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http://dx.doi.org/10.1007/s11033-019-04656-3DOI Listing
February 2019
2 Reads

A report on three live births in women with poor ovarian response following intra-ovarian injection of platelet-rich plasma (PRP).

Mol Biol Rep 2019 Feb 5. Epub 2019 Feb 5.

Endometrium and Endometriosis Research Center, Hamadan University of Medical Sciences, Hamadan, Iran.

The prevalence of poor response to gonadotropin stimulation is approximately 9-24% in women undergoing in vitro fertilization. Interestingly, due to containing a variety of growth factors, platelet-rich plasma (PRP) can play an important role in tissue regeneration and healing. Thus, in this research, we aimed to investigate the intra-ovarian injection of PRP in women with poor ovarian response. Read More

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http://dx.doi.org/10.1007/s11033-019-04609-wDOI Listing
February 2019
1 Read

Genome-wide identification and expression analysis of brassinosteroid action-related genes during the shoot growth of moso bamboo.

Mol Biol Rep 2019 Feb 5. Epub 2019 Feb 5.

State Forestry Administration Key Open Laboratory on the Science and Technology of Bamboo and Rattan, Institute of Gene Science for Bamboo and Rattan Resources, International Centre for Bamboo and Rattan, Beijing, 100102, China.

Brassinosteroids (BRs) are a group of plant steroid hormones that play crucial roles in a range of plant growth and development processes. BR action includes active BR formation by a complex biosynthesis process and driving BR biological function through signal transduction. Although the characterization of several BR action-related genes has been conducted in a few model plants, systematic information about these genes in bamboo is still lacking. Read More

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http://dx.doi.org/10.1007/s11033-019-04642-9DOI Listing
February 2019
1 Read

Silencing the OCT4-PG1 pseudogene reduces OCT-4 protein levels and changes characteristics of the multidrug resistance phenotype in chronic myeloid leukemia.

Mol Biol Rep 2019 Feb 5. Epub 2019 Feb 5.

Post-Graduate Program in Physiological Sciences - PPGCF, Federal University of Rio Grande -FURG, Avenue Itália, Km 8, Rio Grande, RS, Zip Code 96203-900, Brazil.

Cancer stem cells show epigenetic plasticity and intrinsic resistance to anti-cancer therapy, rendering capable of initiating cancer relapse and progression. Transcription factor OCT-4 regulates various pathways in stem cells, but its expression can be regulated by pseudogenes. This work evaluated how OCT4-PG1 pseudogene can affect OCT-4 expression and mechanisms related to the multidrug resistance (MDR) phenotype in FEPS cells. Read More

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http://dx.doi.org/10.1007/s11033-019-04639-4DOI Listing
February 2019
1 Read

Angiogenic regeneration defines loco-regional recurrence following pre-operative radio-chemotherapy for rectal cancer: a pilot study.

Mol Biol Rep 2019 Feb 5. Epub 2019 Feb 5.

Department of Pathology, Democritus University of Thrace, 68100, Alexandroupolis, Greece.

Previous studies from our group have brought forward the concept of angiogenic regeneration during radiotherapy (RT), as a major cause of RT failure. This process was examined herein in rectal cancer patients undergoing preoperative chemo-radiotherapy. Out of 25 patients with stage II/III rectal adenocarcinoma, 15 had incomplete response (pIR) after preoperative chemo-radiotherapy. Read More

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http://dx.doi.org/10.1007/s11033-019-04668-zDOI Listing
February 2019
1 Read

ELEquant: a developmental framework and validation of forensic and conservation real-time PCR assays.

Mol Biol Rep 2019 Feb 5. Epub 2019 Feb 5.

Department of Chemistry, Physics and Forensic Science, Arcadia University, 450 S. Easton Road, Glenside, PA, 19038, USA.

A framework for the development and validation of a qPCR assay for species identification and DNA quantification for conservation and forensic purposes is presented. Elephants are commonly poached for their ivory tusks, which is the primary driving force behind their endangered status. In addition to poaching and trade, habitat loss due to logging and mining has also resulted in loss of elephants. Read More

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http://link.springer.com/10.1007/s11033-019-04660-7
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http://dx.doi.org/10.1007/s11033-019-04660-7DOI Listing
February 2019
2 Reads

Vitamin D status and vitamin D receptor gene polymorphism in Saudi children with acute lower respiratory tract infection.

Mol Biol Rep 2019 Feb 5. Epub 2019 Feb 5.

Pharmacology Department, Faculty of Medicine, Cairo University, Cairo, Egypt.

There is a significant association exists between vitamin D deficiencies, low respiratory tract infections, and certain types of VDR gene polymorphism. Various studies are being conducted to prove any such link between the different clinical conditions due to disturbed vitamin D regulation and VDR gene polymorphisms. The present study analyzed the presence of vitamin D receptor (VDR) gene polymorphisms (ApaI and TaqI) in Saudi pediatric patient suffering from acute lower respiratory tract infection (ALRTI) cases. Read More

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http://dx.doi.org/10.1007/s11033-019-04645-6DOI Listing
February 2019
1 Read

Antiproliferative and apoptotic effects of caffeic acid on SK-Mel-28 human melanoma cancer cells.

Mol Biol Rep 2019 Feb 4. Epub 2019 Feb 4.

PPGBtox, CCNE, Federal University of Santa Maria, Santa Maria, RS, Brazil.

Cutaneous melanoma (CM) is an extremely aggressive cancer presenting low survival and high mortality. The vast majority of patients affected by this disease does not respond or show resistance to the chemotherapeutic drugs, which makes the treatment ineffective. In this sense, the necessity for the development of new agents to assist in CM therapy is extremely important. Read More

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http://link.springer.com/10.1007/s11033-019-04658-1
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http://dx.doi.org/10.1007/s11033-019-04658-1DOI Listing
February 2019
9 Reads

Association of rs6259 polymorphism with SHBG levels and Poly Cystic Ovary Syndrome in Indian population: a case control study.

Mol Biol Rep 2019 Feb 4. Epub 2019 Feb 4.

Centre for Medical Biotechnology, Maharshi Dayanand University, Rohtak, 124001, India.

Polycystic ovary syndrome (PCOS) is the most common endocrinopathy of reproductive-aged women. PCOS reflects a number of possible etiologies but its pathophysiology is still unclear. The principal abnormality of the syndrome is hyperandrogenism (70-80%). Read More

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http://dx.doi.org/10.1007/s11033-019-04665-2DOI Listing
February 2019
1 Read

Elucidation of marine fungi derived anthraquinones as mycobacterial mycolic acid synthesis inhibitors: an in silico approach.

Mol Biol Rep 2019 Feb 4. Epub 2019 Feb 4.

Department of Biosciences, Integral University, Lucknow, UP, 226026, India.

Tuberculosis (TB) is a leading cause of mortality amongst infectious diseases. While the anti-TB drugs can cure TB, the non-compliance and rapidly increasing resistance is of serious concern. The study aimed to search novel potent inhibitor(s) against MabA and PKS18 targets of Mycobacterium tuberculosis (M. Read More

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http://link.springer.com/10.1007/s11033-019-04621-0
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http://dx.doi.org/10.1007/s11033-019-04621-0DOI Listing
February 2019
1 Read

Mutation analysis of genes related to methylmalonic acidemia: identification of eight novel mutations.

Mol Biol Rep 2019 Feb 2. Epub 2019 Feb 2.

Department of Medical Laboratory Sciences, Varastegan Institute for Medical Sciences, Mashhad, Iran.

Methylmalonic acidemia (MMA), an inherited metabolic disease, results from genetic defects in methylmalonyl-CoA mutase or any of the proteins involved in adenosylcobalamin synthesis. This enzyme is classified into several complementation groups and genotypic classes. In this work we explain the biochemical, structural and genetic analysis of 25 MMA patients, from Iran. Read More

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http://dx.doi.org/10.1007/s11033-018-4469-0DOI Listing
February 2019

Enhanced purification of histidine-tagged carboxymethylcellulase produced by Escherichia coli BL21/LBH-10 and comparison of its characteristics with carboxymethylcellulase without histidine-tag.

Mol Biol Rep 2019 Feb 2. Epub 2019 Feb 2.

Department of Applied Biology, Graduate School, Dong-A University, Busan, 49315, Korea.

To enhance purification yield of the carboxymethylcellulase (CMCase) of P. aquimaris LBH-10, E. coli BL21/LBH-10 was constructed to produce the six histidine-tagged CMCase (CMCase with a His-tag). Read More

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http://dx.doi.org/10.1007/s11033-019-04647-4DOI Listing
February 2019
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Interpretation of the effect of CYP2C9, VKORC1 and CYP4F2 variants on warfarin dosing adjustment in Turkey.

Mol Biol Rep 2019 Feb 2. Epub 2019 Feb 2.

Department of Molecular Medicine, Aziz Sancar Institute of Experimental Medicine, Istanbul University, Istanbul, Turkey.

It was aimed to underline the importance and explain the meaning of genetic testing in warfarin dosing and investigate and evaluate the contributions of the CYP2C9, VKORC1, and CYP4F2 variants in a Turkish population. Two hundred patients were genotyped for CYP2C9 (rs1799853, rs1057910 and rs56165452), VKORC1 (rs9934438, rs8050894, rs9923231, rs7294 and rs2359612) and CYP4F2 (rs2108622), yet, only 127 patients were found suitable for further evaluation in terms of their personal response to warfarin due to long term usage and available INR and dose usage information. The DNA sequences were determined by the ABI PRISM 3100 Genetic Analyzer to 3130xl System (Applied Biosystems, Foster City, California). Read More

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http://link.springer.com/10.1007/s11033-019-04634-9
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http://dx.doi.org/10.1007/s11033-019-04634-9DOI Listing
February 2019
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Genome-wide identification of microRNA signatures associated with stem/progenitor cells in Philadelphia chromosome-positive acute lymphoblastic leukemia.

Mol Biol Rep 2019 Feb 2. Epub 2019 Feb 2.

Biotechnology Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, Iran.

Acute lymphoblastic leukemia (ALL) is a malignant transformation with uncontrolled proliferation of lymphoid precursor cells within bone marrow including a dismal prognosis after relapse. Survival of a population of quiescent leukemia stem cells (LSCs, also termed leukemia-initiating cells (LICs)) after treatment is one of the relapse reasons in Ph ALL patient. MicroRNAs (miRNAs) are known as highly conserved 19-24 nucleotides non-protein-coding small RNAs that regulate the expression of human genes. Read More

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http://dx.doi.org/10.1007/s11033-019-04600-5DOI Listing
February 2019
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Do ACE and CKMM gene variations have potent effects on physical performance in inactive male adolescents?

Mol Biol Rep 2019 Feb 1. Epub 2019 Feb 1.

Department of Physical Education and Sport Sciences, Tabriz Branch, Islamic Azad University, Tabriz, Iran.

We studied to ascertain whether the ACE and/or CKMM genotypes independently influence the baseline level of some sport performances in 613 inactive male adolescents (mean ± SD age: 13.24 ± 0.28 years). Read More

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http://link.springer.com/10.1007/s11033-019-04636-7
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http://dx.doi.org/10.1007/s11033-019-04636-7DOI Listing
February 2019
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Anti-proliferative and anti-apoptotic potential effects of epigallocatechin-3-gallate and/or metformin on hepatocellular carcinoma cells: in vitro study.

Mol Biol Rep 2019 Feb 1. Epub 2019 Feb 1.

Biotechnology Department, Faculty of Science, Sebha University, Sabha, Libya.

The effects of epigallocatechin-3-gallate (EGCG) and metformin single treatment have been tested against hepatocellular carcinoma (HCC). This study aimed to assess the combination effects of EGCG and metformin on proliferation and apoptosis of HepG2cells and identified new potential molecular targets. The effect of EGCG and metformin against cell proliferation in HepG2 was determined using MTT assay. Read More

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http://dx.doi.org/10.1007/s11033-019-04653-6DOI Listing
February 2019
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The effects of Pueraria mirifica extract, diadzein and genistein in testosterone-induced prostate hyperplasia in male Sprague Dawley rats.

Mol Biol Rep 2019 Feb 1. Epub 2019 Feb 1.

Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603, Kuala Lumpur, Malaysia.

Pueraria mirifica (PM) is a medicinal plant native to Thailand contained high amount of phytoestrogen and possesses anticancer activity. This study reports the effect of P. mirifica extract, phytoestrogen of diadzein and genistein for its benign prostate hyperplasia properties in testosterone-induced prostate hyperplasia in male Sprague Dawley rats. Read More

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http://dx.doi.org/10.1007/s11033-019-04638-5DOI Listing
February 2019

Characterization of aneuploidy in interspecific hybrid between Urochloa ruziziensis (R. Germ. & Evrard) Crins and Urochloa decumbens (Stapf) R. D. Webster.

Mol Biol Rep 2019 Feb 1. Epub 2019 Feb 1.

Department of Biology/DBI-Plant Cytogenetics Laboratory, Federal University of Lavras (UFLA), Lavras, Minas Gerais, Brazil.

The aim of the study was to characterize the type of aneuploidy present in the hybrid Urochloa ruziziensis × Urochloa decumbens and to confirm the origin of the additional chromosomes through comparative analysis of the hybrid and parental karyotypes. C and CMA banding techniques were used for chromosome differentiation. The parental genotypes showed 36 chromosomes. Read More

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http://dx.doi.org/10.1007/s11033-019-04643-8DOI Listing
February 2019

Genome-wide identification and characterization of Hsp70 gene family in Nicotiana tabacum.

Mol Biol Rep 2019 Feb 1. Epub 2019 Feb 1.

Key Laboratory for Cultivation of Tobacco Industry, College of Tobacco Science, Henan Agricultural University, Zhengzhou, China.

Heat shock proteins 70 (Hsp70) constitute a highly conserved protein family of cellular chaperones widely distributed in plants, where they play a fundamental role in response to biotic and abiotic stress. Until now, genome-wide analyses of the Hsp70 gene family have been conducted for some species. However, reports about Hsp70 genes in Nicotiana tabacum are scarce. Read More

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http://dx.doi.org/10.1007/s11033-019-04644-7DOI Listing
February 2019
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Effect of intravenous injection of antagomiR-1 on brain ischemia.

Mol Biol Rep 2019 Feb 1. Epub 2019 Feb 1.

Faculty of Life Sciences and Biotechnology, Shahid Beheshti University, Tehran, Iran.

Stroke is one of the leading causes of death in the world, but the underlying molecular mechanism of this disease remains elusive, thus it will be great challenges to finding appropriate protection. MicroRNAs are short, single-stranded, non-coding RNAs and recent studies have shown that they are aberrantly expressed in ischemic condition. Due to the fact that miR-1 has harmful effects on neural damages during brain ischemia, limited miR-1 has been proven to be protective in middle cerebral artery occlusion (MCAO). Read More

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http://dx.doi.org/10.1007/s11033-018-04580-yDOI Listing
February 2019
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Optimization of Agrobacterium-mediated transformation in spring bread wheat using mature and immature embryos.

Mol Biol Rep 2019 Feb 1. Epub 2019 Feb 1.

ICAR-Indian Institute of Wheat and Barley Research (IIWBR), Karnal, Haryana, 132001, India.

Wheat is the most widely grown staple food crop in the world and accounts for dietary needs of more than 35% of the human population. Current status of transgenic wheat development is slow all over the world due to the lack of a suitable transformation system. In the present study, an efficient and reproducible Agrobacterium-mediated transformation system in bread wheat (Triticum aestivum L. Read More

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http://dx.doi.org/10.1007/s11033-019-04637-6DOI Listing
February 2019
2.024 Impact Factor

Identification of human endogenous retrovirus transcripts in Hodgkin Lymphoma cells.

Mol Biol Rep 2019 Feb 1. Epub 2019 Feb 1.

University Clinic and Outpatient Clinic for Pediatrics I, Martin Luther University Halle-Wittenberg, Ernst-Grube-Str. 40, 06120, Halle, Germany.

During the last decades, the prognosis for patients with Hodgkin Lymphoma (HL) has been steadily improved. Nevertheless, new and less toxic therapy strategies have to be developed especially for patients with advanced disease. The activation of human endogenous retroviruses (HERV) is suspected to occur in HL and therefore, HERV might represent interesting target structures. Read More

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http://link.springer.com/10.1007/s11033-019-04640-x
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http://dx.doi.org/10.1007/s11033-019-04640-xDOI Listing
February 2019
3 Reads