2,901,878 results match your criteria Molecular & cellular proteomics : MCP[Journal]


Management of Antibiotic-Resistant Helicobacter pylori Infection: Perspectives from Vietnam.

Gut Liver 2019 Apr 23. Epub 2019 Apr 23.

Department of Environmental and Preventive Medicine, Oita University Faculty of Medicine, Oita, Japan.

Antibiotic resistance is the most important factor leading to the failure of eradication regimens. This review focuses on the prevalence of primary and secondary resistance to clarithromycin, metronidazole, amoxicillin, levofloxacin, tetracycline, and multidrug in Vietnam. We searched the PubMed, EMBASE, Vietnamese National Knowledge Infrastructure, and Vietnamese Biomedical databases from January 2000 to December 2016. Read More

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http://dx.doi.org/10.5009/gnl18137DOI Listing
April 2019
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Targeted Knock-In Mice with a Human Mutation in GRTH/DDX25 Reveals the Essential Role of Phosphorylated GRTH in Spermatid Development during Spermatogenesis.

Hum Mol Genet 2019 Apr 22. Epub 2019 Apr 22.

Section on Molecular Endocrinology, Division of Developmental Biology, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA.

Gonadotropin-regulated testicular RNA helicase (GRTH/DDX25) is a testis specific member of the DEAD-box family of RNA helicases expressed in meiotic and haploid germ cells which plays an essential role in spermatogenesis. There are two species of GRTH the 56 kDa non-phospho and 61 kDa phospho forms. Our early studies revealed a missense mutation (R242H) of GRTH in azoospermic men that when expressed in COS1-cells lack the phospho-form of GRTH. Read More

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https://academic.oup.com/hmg/advance-article/doi/10.1093/hmg
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http://dx.doi.org/10.1093/hmg/ddz079DOI Listing
April 2019
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Molecular imaging identifies age-related attenuation of acetylcholine in retrosplenial cortex in response to acetylcholinesterase inhibition.

Neuropsychopharmacology 2019 Apr 22. Epub 2019 Apr 22.

Department of Pharmaceutical Biosciences, Medical Mass Spectrometry Imaging, National Resource for Mass Spectrometry Imaging, Science for Life Laboratory, Uppsala University, Box 591, Uppsala, SE, 75124, Sweden.

The neurotransmitter of the cholinergic system, acetylcholine plays a major role in the brain's cognitive function and is involved in neurodegenerative disorders. Here, we present age-related alterations of acetylcholine levels after administration of the acetylcholinesterase inhibitor drug tacrine in normal mice. Using a quantitative, robust and molecular-specific mass spectrometry imaging method we found that tacrine administration significantly raised acetylcholine levels in most areas of sectioned mice brains, inter alia the striatum, hippocampus and cortical areas. Read More

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http://dx.doi.org/10.1038/s41386-019-0397-5DOI Listing

Socioeconomic position, lifestyle habits and biomarkers of epigenetic aging: a multi-cohort analysis.

Aging (Albany NY) 2019 Apr;11(7):2045-2070

See ACKNOWLEDGMENTS AND FUNDING.

Differences in health status by socioeconomic position (SEP) tend to be more evident at older ages, suggesting the involvement of a biological mechanism responsive to the accumulation of deleterious exposures across the lifespan. DNA methylation (DNAm) has been proposed as a biomarker of biological aging that conserves memory of endogenous and exogenous stress during life.We examined the association of education level, as an indicator of SEP, and lifestyle-related variables with four biomarkers of age-dependent DNAm dysregulation: the total number of stochastic epigenetic mutations (SEMs) and three epigenetic clocks (Horvath, Hannum and Levine), in 18 cohorts spanning 12 countries. Read More

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http://dx.doi.org/10.18632/aging.101900DOI Listing

Atomistic molecular dynamics simulations of the LCST conformational transition in poly(N-vinylcaprolactam) in water.

J Mol Graph Model 2019 Apr 12;90:51-58. Epub 2019 Apr 12.

School of Chemistry, V.N. Karazin Kharkiv National University, 4 Svobody Square, Kharkiv, 61022, Ukraine. Electronic address:

Thermoresponsive poly(N-vinylcaprolactam) (PVCL) has received growing interest due to a temperature-induced phase transition, which switches its solubility in aqueous solutions. However, the lower critical solution temperature (LCST) of PVCL is greatly influenced by the molecular weight, morphology and the environment. Therefore, despite of numerous experimental studies of the thermal response of PVCL, a driving force and a molecular origin of conformation transitions in solution remain far less studied. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S10933263193010
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http://dx.doi.org/10.1016/j.jmgm.2019.04.004DOI Listing
April 2019
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C9orf72 repeat expansions in South Africans with amyotrophic lateral sclerosis.

J Neurol Sci 2019 Apr 17;401:51-54. Epub 2019 Apr 17.

Neurology research group, Department of Medicine, University of Cape Town, Cape Town, South Africa; Division of Neurology, Department of Medicine, University of Cape Town, Cape Town, South Africa. Electronic address:

The hexanucleotide repeat expansion in the C9orf72 gene is the most common genetic variant found in individuals with sporadic amyotrophic lateral sclerosis (ALS), occurring at a frequency of between 7 and 11% in cohorts of European ancestry. While limited data suggest that C9-expansions (>30 repeats) are less frequent in African-Americans with ALS, there is no data on the frequency of C9-expansions among ALS subjects residing in Africa. We therefore investigated the frequency of this expansion mutation (using repeat-primed PCR) in a cohort of 143 South Africans (SA) with ALS. Read More

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http://dx.doi.org/10.1016/j.jns.2019.04.026DOI Listing

Induction of apoptosis in CD4(+) T-cells is linked with optimal treatment response in patients with relapsing-remitting multiple sclerosis treated with Glatiramer acetate.

J Neurol Sci 2019 Mar 29;401:43-50. Epub 2019 Mar 29.

Multiple Sclerosis Center, B' Neurological University Department, AHEPA General Hospital, Aristotle University of Thessaloniki, Thessaloniki, Greece. Electronic address:

Background: Induction of T-cell apoptosis constitutes a mechanism of action for Glatiramer Acetate (GA). We investigated whether activation of apoptotic T-cell death may be indicative of optimal treatment response in patients with relapsing-remitting Multiple Sclerosis (RRMS), with respect to radiological activity.

Methods: We studied apoptotic markers on blood T-cells of forty patients with RRMS, 19 patients under GA and 21 patients under interferon-β (IFNβ), by flow cytometry. Read More

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http://dx.doi.org/10.1016/j.jns.2019.03.030DOI Listing

Carbon black-induced detrimental effect on osteoblasts at low concentrations: Remarkably compromised differentiation without significant cytotoxicity.

Ecotoxicol Environ Saf 2019 Apr 19;178:211-220. Epub 2019 Apr 19.

Beijing Jishuitan Hospital, Peking University Health Science Center, Beijing, 100035, PR China. Electronic address:

Due to similar aerodynamic and micro-nano sized properties between airborne particles and synthetic nanoparticles, a large number of studies have been conducted using carbon-based particles, such as carbon black (CB), carbon nanotubes and graphite, in order to achieve deeper understandings of their adverse effects on human health. It has been reported that particulate matters can aggravate morbidity of patients suffering from bone and joint diseases, e.g. Read More

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http://dx.doi.org/10.1016/j.ecoenv.2019.04.029DOI Listing

miR-125b Upregulates miR-34a and Sequentially Activates Stress Adaption and Cell Death Mechanisms in Multiple Myeloma.

Mol Ther Nucleic Acids 2019 Mar 13;16:391-406. Epub 2019 Mar 13.

Department of Precision Medicine, University of Campania "Luigi Vanvitelli," 80138 Naples, Italy; IRGS, Biogem, Molecular and Precision Oncology Laboratory, Via Camporeale, 83031 Ariano Irpino, Italy. Electronic address:

miR-125b, ubiquitously expressed and frequently dysregulated in several tumors, has gained special interest in the field of cancer research, displaying either oncogenic or oncosuppressor potential based on tumor type. We have previously demonstrated its tumor-suppressive role in multiple myeloma (MM), but the analysis of molecular mechanisms needs additional investigation. The purpose of this study was to explore the effects of miR-125b and its chemically modified analogs in modulating cell viability and cancer-associated molecular pathways, also focusing on the functional aspects of stress adaptation (autophagy and senescence), as well as programmed cell death (apoptosis). Read More

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http://dx.doi.org/10.1016/j.omtn.2019.02.023DOI Listing

Antibacterial activity study of 1,2,4-triazole derivatives.

Eur J Med Chem 2019 Apr 16;173:274-281. Epub 2019 Apr 16.

Shanghai Key Laboratory of Molecular Imaging, Shanghai University of Medicine and Health Sciences, Shanghai, PR China. Electronic address:

Antibiotics are commonly used to fight against bacterial infections, but bacteria have already been resistant to almost all antibiotics due to abuse of antibiotics. 1,2,4-Triazole derived compounds possess chemotherapeutic effects including potential antibacterial activities against drug-sensitive as well as drug-resistant pathogens. Hybridization displays a high potential to develop novel drugs with the capacity to overcome drug resistance, reduce toxicity and improve pharmacokinetic profiles. Read More

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http://dx.doi.org/10.1016/j.ejmech.2019.04.043DOI Listing

Synthesis and immunological studies of β-1,2-mannan-peptide conjugates as antifungal vaccines.

Eur J Med Chem 2019 Apr 5;173:250-260. Epub 2019 Apr 5.

Department of Chemistry, University of Florida, 214 Leigh Hall, Gainesville, FL, 32611, USA. Electronic address:

Fungal cell surface carbohydrates and proteins are useful antigens for the development of antifungal vaccines. In this study, glycopeptides consisting of the β-1,2-mannan and N-terminal peptide epitopes of Candida albicans (C. albicans) cell wall phosphomannan complex and Als1p (rAls1p-N) protein, respectively, were synthesized and covalently conjugated with keyhole limpet hemocyanin (KLH) and human serum albumin (HSA) through homobifunctional disuccinimidyl glutarate. Read More

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http://dx.doi.org/10.1016/j.ejmech.2019.04.001DOI Listing

Design, synthesis and docking studies of benzimidazole derivatives as potential EGFR inhibitors.

Eur J Med Chem 2019 Apr 9;173:240-249. Epub 2019 Apr 9.

Ankara University, Faculty of Pharmacy, Department of Pharmacology, 06100, Tandoğan-Ankara, Turkey.

In this study, a series of benzimidazoles bearing thiosemicarbazide chain or triazole and thiadiazole rings were designed and synthesized. Crystal and molecular structure of the compound 5c has been characterized by single crystal X-ray crystallographic analysis. EGFR kinase inhibitory potencies of synthesized compounds were compared with erlotinib in vitro and most of the compounds exhibited significant activities. Read More

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http://dx.doi.org/10.1016/j.ejmech.2019.04.012DOI Listing

A pilot study on the efficacy of transcranial direct current stimulation applied to the pharyngeal motor cortex for dysphagia associated with brainstem involvement in multiple sclerosis.

Clin Neurophysiol 2019 Apr 11;130(6):1017-1024. Epub 2019 Apr 11.

Unit of Neurology and Neurorehabilitation, IRCCS Neuromed, Via Atinense 18, 86077 Pozzilli, IS, Italy.

Objective: we investigated the effect of anodal transcranial direct current stimulation (tDCS) applied over the pharyngeal motor area in dysphagia associated with multiple sclerosis (MS).

Methods: Eighteen MS patients with dysphagia associated with brainstem involvement were randomized to receive either "real" or "sham" tDCS.

Primary Outcome: The Penetration/Aspiration Scale (PAS). Read More

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http://dx.doi.org/10.1016/j.clinph.2019.04.003DOI Listing

Untargeted LC/MS-based metabolic phenotyping (metabonomics/metabolomics): The state of the art.

J Chromatogr B Analyt Technol Biomed Life Sci 2019 Apr 8;1117:136-147. Epub 2019 Apr 8.

Division of Computational and Systems Medicine, Department of Surgery and Cancer, Imperial College, Exhibition Road, South Kensington, London SW7 2AZ, UK. Electronic address:

Liquid chromatography (LC) hyphenated to mass spectrometry is currently the most widely used means of determining metabolic phenotypes via both untargeted and targeted analysis. At present a range of analytical separations, including reversed-phase, hydrophilic interaction and ion-pair LC are employed to maximise metabolome coverage with ultra (high) performance liquid chromatography (UHPLC) increasingly displacing conventional high performance liquid chromatography because of the need for short analysis times and high peak capacity in such applications. However, it is widely recognized that these methodologies do not entirely solve the problems facing researchers trying to perform comprehensive metabolic phenotyping and in addition to these "routine" approaches there are continuing investigations of alternative separation methods including 2-dimensional/multi column approaches. Read More

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http://dx.doi.org/10.1016/j.jchromb.2019.04.009DOI Listing

A selectivity study of benzenesulfonamide derivatives on human carbonic anhydrase II/IX by 3D-QSAR, Molecular Docking and Molecular Dynamics Simulation.

Comput Biol Chem 2019 Apr 4;80:234-243. Epub 2019 Apr 4.

School of Pharmacy and Bioengineering, Chongqing University of Technology, Chongqing, 400054, China; Chongqing Key Laboratory of Targeted Drug Screening and Activity Evaluation, Chongqing, 400054, China; Chongqing Key Laboratory of Medicinal Chemistry and Molecular Pharmacology, Chongqing, 400054, China. Electronic address:

Nowadays, different approaches have been pursued with the intent to develop sulfonamide-like carbonic anhydrase inhibitors that possess better selectivity profiles toward the different human isoforms of the enzyme. Here, we used conventional 3D-QSAR methods, including comparative molecular field analysis (CoMFA), comparative molecular similarity indices analysis (CoMSIA), and Topomer CoMFA, to construct three-dimensional quantitative structure-activity relationship (3D-QSAR) models for benzenesulfonamide derivatives as human carbonic anhydrase (hCA) II/IX inhibitors. The theoretical models had good reliability (R>0. Read More

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http://dx.doi.org/10.1016/j.compbiolchem.2019.03.005DOI Listing

Role of nuclear RNA in regulating chromatin structure and transcription.

Curr Opin Cell Biol 2019 Apr 19;58:120-125. Epub 2019 Apr 19.

MRC Human Genetics Unit, Institute of Genetics and Molecular Medicine, University of Edinburgh, Edinburgh EH4 2XU, UK. Electronic address:

The importance of three-dimensional chromatin organisation in genome regulation has never been clearer. But in spite of the enormous technological advances to probe chromatin organisation in vivo, there is still a lack of mechanistic understanding of how such an arrangement is achieved. Here we review emerging evidence pointing to an intriguing role of nuclear RNA in shaping large-scale chromatin structure and regulating genome function. Read More

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http://dx.doi.org/10.1016/j.ceb.2019.03.007DOI Listing

Tip of the trichome: evolution of acylsugar metabolic diversity in Solanaceae.

Curr Opin Plant Biol 2019 Apr 19;49:8-16. Epub 2019 Apr 19.

Department of Biochemistry and Molecular Biology, Michigan State University, East Lansing, MI 48824, USA; Department of Plant Biology, Michigan State University, East Lansing, MI 48824, USA. Electronic address:

Acylsugars are insecticidal plant specialized metabolites produced in the Solanaceae (nightshade family). Despite having simple constituents, these compounds are unusually structurally diverse. Their structural variations in phylogenetically closely related species enable comparative biochemical approaches to understand acylsugar biosynthesis and pathway diversification. Read More

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http://dx.doi.org/10.1016/j.pbi.2019.03.005DOI Listing

Salvianolic acid B inhibits Ang II-induced VSMC proliferation in vitro and intimal hyperplasia in vivo by downregulating miR-146a expression.

Phytomedicine 2018 Nov 12;58:152754. Epub 2018 Nov 12.

Department of Biochemistry and Molecular Biology, The Key Laboratory of Neural and Vascular Biology, Ministry of Education of China, Hebei Medical University, Shijiazhuang, Hebei 050017, China. Electronic address:

Background: Salvianolic acid B (Sal B), a water-soluble compound extracted from Salvia miltiorrhiza that has been widely used to treat cardiovascular diseases for hundreds of years in China, exerts cardiovascular protection by multiple mechanisms. miR-146a is involved in vascular smooth muscle cell (VSMC) phenotypic modulation and proliferation. However, it has yet to be investigated whether the cardiovascular protective effect of Sal B is mediated by miR-146a. Read More

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http://dx.doi.org/10.1016/j.phymed.2018.11.014DOI Listing
November 2018

PML nuclear bodies are recruited to persistent DNA damage lesions in an RNF168-53BP1 dependent manner and contribute to DNA repair.

DNA Repair (Amst) 2019 Apr 6;78:114-127. Epub 2019 Apr 6.

Department of Genome Integrity, Institute of Molecular Genetics, v.v.i., Academy of Sciences of the Czech Republic, Prague, Czech Republic; Laboratory of Genome Integrity, Institute of Molecular and Translational Medicine, Palacky University, Olomouc, Czech Republic; Genome Integrity Unit, Danish Cancer Society Research Center, Copenhagen, Denmark; Science for Life Laboratory, Division of Genome Biology, Department of Medical Biochemistry and Biophysics, Karolinska Institutet, S-171 21, Stockholm, Sweden. Electronic address:

The bulk of DNA damage caused by ionizing radiation (IR) is generally repaired within hours, yet a subset of DNA lesions may persist even for long periods of time. Such persisting IR-induced foci (pIRIF) co-associate with PML nuclear bodies (PML-NBs) and are among the characteristics of cellular senescence. Here we addressed some fundamental questions concerning the nature and determinants of this co-association, the role of PML-NBs at such sites, and the reason for the persistence of DNA damage in human primary cells. Read More

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http://dx.doi.org/10.1016/j.dnarep.2019.04.001DOI Listing

The non-anticoagulant promise of heparin and its mimetics.

Authors:
Barbara Mulloy

Curr Opin Pharmacol 2019 Apr 19;46:50-54. Epub 2019 Apr 19.

Institute of Pharmaceutical Science, King's College London, 150 Stamford Street, London, SE1 9NH, UK. Electronic address:

Heparin, the widely used anticoagulant and antithrombotic polysaccharide, has other potential therapeutic uses that arise from its similarity to heparan sulfate. This review provides a brief overview of the most recent developments in this field, paying particular respect to pulmonary and respiratory pharmacology. It has often been said that heparin, with its mimetics and derivatives, shows great promise in the treatment of inflammatory, infectious, and malignant conditions. Read More

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http://dx.doi.org/10.1016/j.coph.2019.03.009DOI Listing

Generation of the human induced pluripotent stem cell (hiPSC) line PSMi005-A from a patient carrying the KCNQ1-R190W mutation.

Stem Cell Res 2019 Apr 13;37:101437. Epub 2019 Apr 13.

Coronary Care Unit, Laboratory of Experimental Cardiology for Cell and Molecular Therapy, Fondazione IRCCS Policlinico San Matteo, Pavia, Italy; Department of Molecular Medicine, Unit of Cardiology, Università degli studi di Pavia, Pavia, Italy; Department of Medicine, University of Cape Town, Cape Town, South Africa. Electronic address:

We generated human induced pluripotent stem cells (hiPSCs) from dermal fibroblasts of a woman carrier of the heterozygous mutation c.568C > T p.R190W on the KCNQ1 gene. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S18735061193006
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http://dx.doi.org/10.1016/j.scr.2019.101437DOI Listing
April 2019
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Characterizing the role of tissue-type plasminogen activator in a mouse model of Group A streptococcal infection.

Microbes Infect 2019 Apr 19. Epub 2019 Apr 19.

Illawarra Health and Medical Research Institute and School of Chemistry and Molecular Bioscience, Molecular Horizons, University of Wollongong, Wollongong, New South Wales, Australia. Electronic address:

Plasmin(ogen) acquisition is critical for invasive disease initiation by Streptococcus pyogenes (GAS). Host urokinase plasminogen activator (uPA) plays a role in mediating plasminogen activation for GAS dissemination, however the contribution of tissue-type plasminogen activator (tPA) to GAS virulence is unknown. Using novel tPA-deficient ALBPLG1 mice, our study revealed no difference in mouse survival, bacterial dissemination or the pathology of GAS infection in the absence of tPA in AlbPLG1/tPA mice compared to AlbPLG1 mice. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S12864579193005
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http://dx.doi.org/10.1016/j.micinf.2019.04.004DOI Listing
April 2019
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Stathmin regulates the proliferation and odontoblastic/ osteogenic differentiation of human dental pulp stem cells through Wnt/β-catenin signaling pathway.

J Proteomics 2019 Apr 19. Epub 2019 Apr 19.

Department of Stomatology, Nanfang Hospital, Southern Medical University, Guangzhou, PR China; College of Stomatology, Southern Medical University, Guangzhou, PR China. Electronic address:

Osteogenic/ odontoblastic differentiation of human dental pulp stem cells (hDPSCs) is a key factor in tooth and pulp regeneration, but its mechanism still remains unknown. The purpose of this research is to look into the mechanism by which Stathmin affects the proliferation and odontoblastic/ osteogenic differentiation of hDPSCs, and whether the Wnt/β- catenin is related to this regulation. First, the Stathmin expression was inhibited by lentiviral vector, after that the transcriptome sequencing technology was used to screen the differentially expressed genes, Wnt5a which related to the regulation of Wnt/β-catenin was regulated. Read More

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http://dx.doi.org/10.1016/j.jprot.2019.04.014DOI Listing

Risk Factors for Severe Liver Disease in Patients With Type 2 Diabetes.

Clin Gastroenterol Hepatol 2019 Apr 19. Epub 2019 Apr 19.

Department of Upper Gastrointestinal Diseases, Division of Hepatology, Karolinska University Hospital, Stockholm, Sweden; Department of Medicine, Solna, Karolinska Institutet, Stockholm, Sweden. Electronic address:

Background & Aims: Type 2 diabetes is a risk factor for development of cirrhosis and hepatocellular carcinoma. However, risk factor that identify persons with the highest risk for these outcomes are missing from unselected, population-based cohorts.

Methods: The National Diabetes Register contains data on about 90% of persons in Sweden with type 2 diabetes. Read More

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http://dx.doi.org/10.1016/j.cgh.2019.04.038DOI Listing

Nucleophosmin in leukemia: consequences of anchor loss.

Int J Biochem Cell Biol 2019 Apr 19. Epub 2019 Apr 19.

Institute of Hematology and Blood Transfusion, U Nemocnice 1, 128 20 Prague 2, Czech Republic. Electronic address:

Nucleophosmin (NPM), one of the most abundant nucleolar proteins, has crucial functions in ribosome biogenesis, cell cycle control, and DNA-damage repair. In human cells, NPM occurs mainly in oligomers. It functions as a chaperone, undergoes numerous interactions and forms part of many protein complexes. Read More

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http://dx.doi.org/10.1016/j.biocel.2019.04.007DOI Listing

Metabolic and epigenetic reprogramming in the arsenic-induced cancer stem cells.

Semin Cancer Biol 2019 Apr 19. Epub 2019 Apr 19.

Department of Pharmaceutical Sciences, Eugene Applebaum College of Pharmacy and Health Sciences, Wayne State University, 259 Mack Avenue, Detroit, MI, 48201, USA. Electronic address:

At present, the belief that genetic mutations control every aspect of tumorigenesis is still very popular. Even for the highly debated "bad luck" theory of cancers, it ascertained that random mutation of genes during the self-renewal of somatic stem cells is responsible for cancer initiation. Logically, most of the new therapeutic strategies so far, from molecular targeting to precision medicine or personalized medicine, are genome-obsessed and focused on identifying and targeting these mutated genes. Read More

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http://dx.doi.org/10.1016/j.semcancer.2019.04.003DOI Listing

Revisiting SEPT7 and the slippage of β-strands in the septin family.

J Struct Biol 2019 Apr 19. Epub 2019 Apr 19.

São Carlos Institute of Physics, University of São Paulo, São Carlos, São Paulo, Brazil. Electronic address:

Septins are GTP-binding proteins that will often spontaneously assemble into filaments. In some species, particularly budding yeast, it is well known that these are capable of associating with membranes in order to fulfill their cellular role as a component of the cytoskeleton. Different from other human septins, SEPT7 appears to be unique in that it is an essential component of all hetero-oligomeric complexes described to date. Read More

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http://dx.doi.org/10.1016/j.jsb.2019.04.015DOI Listing

A Pilot Metabolomics Study of Tuberculosis Immune Reconstitution Inflammatory Syndrome.

Int J Infect Dis 2019 Apr 19. Epub 2019 Apr 19.

Mycobacterial Research Laboratories, Colorado State University, Fort Collins, CO, USA; Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, CO, USA. Electronic address:

Background: Diagnosis of paradoxical tuberculosis-associated immune reconstitution inflammatory syndrome (TB-IRIS) is challenging and new tools are needed for early diagnosis as well as to understand the biochemical events that underlie the pathology in TB-IRIS.

Methods: Plasma samples were obtained from participants from a randomized HIV/TB treatment strategy study (AIDS Clinical Trials Group [ACTG] A5221) with (n = 26) and without TB-IRIS (n = 22) for an untargeted metabolomics pilot study by liquid-chromatography mass spectrometry. The metabolic profile of these participants was compared at the study entry and as close to the diagnosis of TB-IRIS as possible (TB-IRIS window). Read More

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http://dx.doi.org/10.1016/j.ijid.2019.04.015DOI Listing

Nitric oxide and interactions with reactive oxygen species in the development of melanoma, breast, and colon cancer: A redox signaling perspective.

Nitric Oxide 2019 Apr 19. Epub 2019 Apr 19.

New York University School of Medicine, New York, NY, USA.

Cancer development is closely related to chronic inflammation, which is associated with identifiable markers of tumor progression, such as uncontrolled cell proliferation, angiogenesis, genomic instability, chemotherapeutic resistance, and metastases. Redox processes mediated by reactive oxygen species (ROS) and nitric oxide (NO) within the inflammatory tumor microenvironment play an essential role in directly influencing intercellular and intracellular signaling. These reactive species originating in the cancer cell or its microenvironment, mediate the epithelial-mesenchymal transition (EMT) and the mesenchymal-epithelial transition (MET). Read More

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http://dx.doi.org/10.1016/j.niox.2019.04.009DOI Listing

A sub-chronic Xysmalobium undulatum hepatotoxicity investigation in HepG2/C3A spheroid cultures compared to an in vivo model.

J Ethnopharmacol 2019 Apr 19:111897. Epub 2019 Apr 19.

Pharmacen™, Centre of Excellence for Pharmaceutical Sciences, North-West University, Private Bag X6001, Potchefstroom, 2520, South Africa; Department of Biochemistry and Molecular Biology, University of Southern Denmark, DK-5230, Odense M, Denmark; Celvivo IVS, Blommenslyst, Denmark. Electronic address:

Ethnopharmacology Relevance: Traditional herbal medicines are utilized by 27 million South Africans. Xysmalobium undulatum (Uzara) is one of the most widely used traditional medicinal plants in Southern Africa. A false belief in the safety of herbal medicine may result in liver injury. Read More

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http://dx.doi.org/10.1016/j.jep.2019.111897DOI Listing

Interaction Between Microfilament and Microtubule- Dependent Tensions and Ischemia/Hypoxic-Induced Alteration of Structural Tension in Neuronal Cells.

Brain Res Bull 2019 Apr 19. Epub 2019 Apr 19.

Laboratory Center for Basic Medical Sciences, Nanjing Medical University, Nanjing, PR China. Electronic address:

Intracellular mechanical tension plays a vital role in maintaining neuronal function and is generated steerablely by motor proteins along microfilaments (MFs) and microtubules (MTs). To explore the interaction between these subcellular tensions and elucidate their underlying mechanisms, we constructed MF- and MT-dependent tension probes using the Förster resonance energy transfer technique. Hypotonic stress activated MF and MT tensions in calcium-dependent manner, which antagonized outward expansion of cells synergistically; conversely, hypertonic stress attenuated MF and MT tensions in a calcium-independent manner and their interaction is antagonistical. Read More

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http://dx.doi.org/10.1016/j.brainresbull.2019.04.007DOI Listing

Effective Encapsulation of Curcumin in Nanoparticles Enabled by Hydrogen Bonding Using Flash Nanocomplexation.

Int J Pharm 2019 Apr 19. Epub 2019 Apr 19.

Institute for NanoBioTechnology, Johns Hopkins University, Baltimore, MD, USA; Department of Materials Science and Engineering, Whiting School of Engineering, Johns Hopkins University, Baltimore, MD, USA; Translational Tissue Engineering Center, Johns Hopkins University School of Medicine, Baltimore, MD, USA; Department of Biomedical Engineering, Johns Hopkins School of Medicine, Baltimore, MD, USA. Electronic address:

Nanoparticular system of a model small molecular drug curcumin (CUR) was prepared using a novel method, namely, flash nanocomplexation by hydrogen bonding interactions. The CUR-loaded nanoparticles (NPs) were fabricated by mixing CUR, tannic acid and polyvinyl alcohol (PVA) in aqueous solutions under turbulent condition through a three-inlet confined impinging jet (CIJ) device. Compared to bulk mixing, FNC has the advantage of scalability, reproducibility and without causing the variations by different mixing sequences. Read More

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http://dx.doi.org/10.1016/j.ijpharm.2019.04.053DOI Listing

The first complete mitochondrial genome of a cyclophorid land snail, with implications for architaenioglossan relationships (Mollusca, Caenogastropoda, Cyclophoroidea).

Int J Biol Macromol 2019 Apr 19. Epub 2019 Apr 19.

School of Life Sciences, Nanchang University, Nanchang 330031, China. Electronic address:

Herein, we described the complete mitochondrial genome ('mitogenome') of the Chinese land snail Cyclophorus martensianus, which is the first published mitogenome for the caenogastropod family Cyclophoridae. This mitogenome is 15,308 bp long consisting of 37 genes: 13 protein-coding genes (PCG's), 22 tRNA genes and two rRNA genes. The A + T content (69. Read More

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http://dx.doi.org/10.1016/j.ijbiomac.2019.04.138DOI Listing

Untargeted LC-MS-based metabonomics revealed that aristolochic acid I induces testicular toxicity by inhibiting amino acids metabolism, glucose metabolism, β-oxidation of fatty acids and the TCA cycle in male mice.

Toxicol Appl Pharmacol 2019 Apr 19. Epub 2019 Apr 19.

Chinese Academy of Inspection and Quarantine, Institute of Chemicals Safety, Beijing, China. Electronic address:

As the main toxic component of aristolochic acid, aristolochic acid I (AAI) is primarily found in Aristolochiaceae plants such as Aristolochia, Aristolochia fangchi and Caulis aristolochiae manshuriensis. AAI has been proven to be carcinogenic, mutagenic and nephrotoxic. Although the role of AAI in testicular toxicity has been reported, its mechanism of action is unknown. Read More

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http://dx.doi.org/10.1016/j.taap.2019.04.014DOI Listing

Identification of arylsulfatase B gene mutations and clinical presentations of Iranian patients with Mucopolysaccharidosis VI.

Gene 2019 Apr 19. Epub 2019 Apr 19.

Diabetes Research Center, Health Research Institute, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran; Department of Medical Genetics, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran. Electronic address:

Background: Mucopolysaccharidosis (MPS) type VI, also known as Maroteaux-Lamy syndrome, is an autosomal recessive lysosomal storage disorder caused by a deficiency in arylsulfatase B (ARSB) enzyme. Our objectives were to investigate clinical phenotypes and performed molecular studies in Iranian patients with MPS VI, for the first time, in the southwestern Iran.

Methods: We studied 14 cases from 10 unrelated kindreds with MPS VI that were enrolled during 8 years. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S03781119193040
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http://dx.doi.org/10.1016/j.gene.2019.04.050DOI Listing
April 2019
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Genetic status of indigenous poultry (red jungle fowl) from India.

Gene 2019 Apr 19. Epub 2019 Apr 19.

Department of Biotechnology, Assam University, Silchar, Assam Pin-788011, India; University of Kalyani, Nadia, West Bengal Pin-741235, India. Electronic address:

The global biodiversity of domesticated red jungle fowl (Gallus gallus) is gradually eroding by replacement with commercial poultry breeds and results loss of valuable genetic and physical traits like resistance to disease, extreme environment, etc. posing a threat to the poultry genetic resources. Very fewer reports exist on Indian poultry diversity, especially native chicken of India. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S03781119193040
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http://dx.doi.org/10.1016/j.gene.2019.04.051DOI Listing
April 2019
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Novel association of SNP rs479200 in EGLN1 gene with predisposition to preeclampsia.

Gene 2019 Apr 19. Epub 2019 Apr 19.

Department of Cell Biology and Molecular Genetics, Sri Devaraj Urs Academy of Higher Education and Research, Kolar, India. Electronic address:

Objectives: Placental hypoxia is a hallmark of preeclampsia. SNP rs479200 in the EGLN1 gene is associated with reduced responsiveness to hypoxia. Whether this translates into an association between SNP rs479200 and preeclampsia is not known. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S03781119193040
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http://dx.doi.org/10.1016/j.gene.2019.04.049DOI Listing

Differential expression patterns of sodium potassium ATPase alpha and beta subunit isoforms in mouse brain during postnatal development.

Neurochem Int 2019 Apr 19. Epub 2019 Apr 19.

Ruhr University Bochum, Faculty of Chemistry and Biochemistry, Biochemistry II, Bochum, Germany; Ruhr University Bochum, International Graduate School for Neuroscience, Germany. Electronic address:

The sodium potassium ATPase (Na/K ATPase) is essential for the maintenance of a low intracellular Na and a high intracellular K concentration. Loss of function of the Na/K ATPase due to mutations in Na/K ATPase genes, anoxic conditions, depletion of ATP or inhibition of the Na/K ATPase function using cardiac glycosides such as digitalis, causes a depolarization of the resting membrane potential. While in non-excitable cells, the uptake of glucose and amino acids is decreased if the function of the Na/K ATPase is compromised, in excitable cells the symptoms range from local hyper-excitability to inactivating depolarization. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S01970186183045
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http://dx.doi.org/10.1016/j.neuint.2019.04.009DOI Listing
April 2019
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Differential effects of psychoactive substances on human wildtype and polymorphic T356M dopamine transporters (DAT).

Toxicology 2019 Apr 19. Epub 2019 Apr 19.

Neurotoxicology Research Group, Division Toxicology, Institute for Risk Assessment Sciences (IRAS), Faculty of Veterinary Medicine, Utrecht University, Utrecht, the Netherlands. Electronic address:

Many psychoactive substances affect the human dopamine (DA) reuptake transporter (hDAT). Polymorphisms in the encoding gene could affect the functionality of the transporter and consequently alter effects of psychotropic and recreational drugs. Recently, a T356 M single nucleotide polymorphism in the human SLC6A3 gene was described, which resulted in functional impairments of DA uptake. Read More

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http://dx.doi.org/10.1016/j.tox.2019.04.012DOI Listing

Computer-designed active human butyrylcholinesterase double mutant with a new catalytic triad.

Chem Biol Interact 2019 Apr 19. Epub 2019 Apr 19.

Kazan Federal University, Neuropharmacology Laboratory, Kremlevskaya Str, 18, Kazan, 420008, Russia. Electronic address:

A computer-designed mutant of human butyrylcholinesterase (BChE), N322E/E325G, with a novel catalytic triad was made. The catalytic triad of the wild-type enzyme (S198·H438·E325) was replaced by S198·H438·N322E in silico. Molecular dynamics for 1. Read More

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http://dx.doi.org/10.1016/j.cbi.2019.04.019DOI Listing

Dual actions of norathyriol as a new candidate hypouricaemic agent: uricosuric effects and xanthine oxidase inhibition.

Eur J Pharmacol 2019 Apr 19. Epub 2019 Apr 19.

Biomedical Engineering Research Center, Kunming Medical University, Kunming, 650500, China. Electronic address:

Hyperuricaemia, which results from the overproduction and underexcretion of uric acid, has been linked with chronic renal dysfunction, cardiovascular diseases, diabetes and metabolic syndrome. However, available clinical drugs cannot simultaneously target the production and excretion of uric acid. Norathyriol, a natural xanthone, was expected to have the dual actions. Read More

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http://dx.doi.org/10.1016/j.ejphar.2019.04.034DOI Listing

Progress in agonist therapy for substance use disorders: Lessons learned from methadone and buprenorphine.

Neuropharmacology 2019 Apr 19. Epub 2019 Apr 19.

Molecular Targets and Medications Discovery Branch, National Institute on Drug Abuse, Intramural Research Program, Baltimore, MD, 21224, USA. Electronic address:

Substance use disorders (SUD) are serious public health problems worldwide. Although significant progress has been made in understanding the neurobiology of drug reward and the transition to addiction, effective pharmacotherapies for SUD remain limited and a majority of drug users relapse even after a period of treatment. The United States Food and Drug Administration (FDA) has approved several medications for opioid, nicotine, and alcohol use disorders, whereas none are approved for the treatment of cocaine or other psychostimulant use disorders. Read More

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http://dx.doi.org/10.1016/j.neuropharm.2019.04.015DOI Listing
April 2019
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The sensilla trichodea-biased EoblPBP1 binds sex pheromones and green leaf volatiles in Ectropis obliqua Prout, a geometrid moth pest that uses Type-II sex pheromones.

J Insect Physiol 2019 Apr 19. Epub 2019 Apr 19.

Key Laboratory of Tea Quality and Safety Control, Ministry of Agriculture, Tea Research Institute, Chinese Academy of Agricultural Sciences, Hangzhou, 310008, China. Electronic address:

Pheromone-binding proteins (PBPs) are considered to play critical roles in sex pheromone detection. Lepidopteran moths can be divided into two taxa, those that use Type-I sex pheromones, such as C10-C18 unsaturated aldehydes, alcohols and acetates, and those that use Type-II pheromones, which are C17-C23 polyunsaturated hydrocarbons and their epoxide derivatives. To date, nearly all the characterized PBPs have been reported in moths with Type-I sex pheromones, and the physiological functions of PBPs in moths that use Type-II sex pheromones remains unclear. Read More

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http://dx.doi.org/10.1016/j.jinsphys.2019.04.005DOI Listing

Matrix metalloproteinase-12 produced by Ly6C macrophages prolongs the survival after myocardial infarction by preventing neutrophil influx.

J Mol Cell Cardiol 2019 Apr 19. Epub 2019 Apr 19.

Department of Allergy and Clinical Immunology, Graduate School of Medicine, Chiba University, Chiba, Japan. Electronic address:

Background: Various immune cells are involved in different phases of cardiac repair after myocardial infarction (MI). Especially, Ly6C M2-like macrophages (Ly6C macrophages) are vital for cardiac repair after MI. However, the molecular mechanisms how Ly6C macrophages promote wound healing after MI are still largely unknown. Read More

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http://dx.doi.org/10.1016/j.yjmcc.2019.04.007DOI Listing

A reliable fluorimetric method to screen the nitric oxide synthase inhibitors in 96 well plate.

Anal Biochem 2019 Apr 19. Epub 2019 Apr 19.

Dr. BR Ambedkar Center for Biomedical Research, University of Delhi, Delhi, 110007, India. Electronic address:

In general, 4 amino-5-methylamino-2',7'-difluorescein diacetate (DAF-FM-DA) dye is used to detect nitric oxide in biological systems through cell imaging. In this study, we have used 96 well plate format to quantify nitric oxide using DAF-FM-DA through a multimode reader (or independently using fluorospectrometer) and could be visualized in fluorescence microscope. Similar study otherwise will require a high-end instrument. Read More

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http://dx.doi.org/10.1016/j.ab.2019.04.014DOI Listing

Nanoparticle-Assembled Thin Film with amphipathic nanopores for Organic Solvent Nanofiltration.

ACS Appl Mater Interfaces 2019 Apr 22. Epub 2019 Apr 22.

Polymeric thin film composite (TFC) membranes have been proved promising for a wide range of separation applications. However, their development is significantly hindered by low permeance (below 8.0 L m-2 h-1 bar-1). Read More

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http://pubs.acs.org/doi/10.1021/acsami.9b03753
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http://dx.doi.org/10.1021/acsami.9b03753DOI Listing

Specific Targeting, Imaging and Ablation of Tumor-Associated Macrophages by Theranostic Mannose-AIEgen Conjugates.

Anal Chem 2019 Apr 22. Epub 2019 Apr 22.

Tumor associated macrophages (TAMs) that exist in tumor microenvironment promote tumor progression, and have been suggested as a promising therapeutic target for cancer therapy in pre-clinical studies. Development of theranostic systems capable of specific targeting, imaging, and ablation of TAMs will offer clinical benefits. Here we constructed a theranostic probe, namely TPE-Man, by attaching mannose moieties to a red-emissive and AIE (aggregation-induced emission)-active photosensitizer. Read More

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http://dx.doi.org/10.1021/acs.analchem.9b01053DOI Listing

Characterization of Ethoxylated Alcohols in Friction Reducers using Matrix-Assisted Laser Desorption/Ionization - Time-of-Flight - Mass Spectrometry.

Rapid Commun Mass Spectrom 2019 Apr 22. Epub 2019 Apr 22.

Department of Chemistry and Biochemistry, The University of Texas at Arlington, Arlington, TX, USA.

Rationale: Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) provides detailed information for the analysis of ethoxylated alcohols and polymers. In this study, five friction reducers used in commercial hydraulic fracturing processes were analyzed in their as-received form to identify their ethoxylated alcohol content. The friction reducers were then subjected to lab-simulated downhole conditions. Read More

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http://dx.doi.org/10.1002/rcm.8468DOI Listing

Rapid profiling of triglycerides in human breast milk using LESA-FTMS reveals new VLCFAs and differences within individuals.

Rapid Commun Mass Spectrom 2019 Apr 22. Epub 2019 Apr 22.

Nutrition and Bone Health group MRC HNR, Cambridge, CB1 9NL, United Kingdom.

Rationale: In this paper, we describe a novel method for preparing milk samples and profiling the triglyceride fraction from them. This method was used to explore how the triglyceride (TG) profile of milk modulates as lactation progresses and how the TG profile differs between breasts.

Methods: Fresh milk was spotted onto Whatman filter paper and air-dried. Read More

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http://dx.doi.org/10.1002/rcm.8465DOI Listing

The altered expression of autophagy-related genes participates in heart failure: NRBP2 and CALCOCO2 are associated with left ventricular dysfunction parameters in human dilated cardiomyopathy.

PLoS One 2019 22;14(4):e0215818. Epub 2019 Apr 22.

Cardiocirculatory Unit, Health Research Institute of La Fe University Hospital (IIS La Fe), Valencia, Spain.

This study aimed to analyze changes in the expression of autophagy- and phagocytosis-related genes in patients with dilated cardiomyopathy (DCM), especially in relation to left ventricular (LV) dysfunction. Furthermore, transmission electron microscopy of the diseased tissue was carried out to investigate if the gene expression changes are translated into ultrastructural alterations. LV tissue samples from patients with DCM (n = 13) and from controls (CNT; n = 10) were analyzed by RNA-sequencing, whereupon the altered expression (P < 0. Read More

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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0215818PLOS
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