2,871,598 results match your criteria Molecular & cellular proteomics : MCP[Journal]


[Application of stretched exponential diffusion-weighted imaging model in quantitative diagnosis of nonalcoholic fatty liver disease: in a rabbit model].

Zhonghua Yi Xue Za Zhi 2019 Feb;99(7):500-504

Department of Radiology, Northern Jiangsu People's Hospital, Clinical Medical School of Yangzhou University, Yangzhou 225001, China.

To evaluate the feasibility of diffusion parameters obtained from mono-exponential and stretched exponential diffusion-weighted imaging models in staging of nonalcoholic fatty liver disease and compare the diagnosis ability of nonalcoholic steatohepatitis. Thirty-two 12 -week-old male New Zealand rabbits, about 2.5 kg of each, were randomly divided into five groups. Read More

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http://dx.doi.org/10.3760/cma.j.issn.0376-2491.2019.07.005DOI Listing
February 2019

[Activation of GPER inhibts proliferation and autophagy in prostatic epithelial cells].

Zhonghua Yi Xue Za Zhi 2019 Feb;99(6):419-422

Department of Urology, the First Affiliated Hospital of Guangzhou Medical College, Guangdong Key Laboratory of Urology, Guangzhou 510230, China.

To investigate the effect of G protein-coupled estrogen receptor (GPER) on proliferation and autophagy in prostatic epithelial cells. Cell Counting Kit-8 (CCK8) assay was used to observe the growth of BPH-1 and RWPE-1 cells after treating with or not with estradiol or GPER selective agonist G1. Autophagy was quantified with Western blot and Cyto-ID autophagy detection kit after treating with estradiol, G1 or both G1 and G15 in the same cells. Read More

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http://dx.doi.org/10.3760/cma.j.issn.0376-2491.2019.06.007DOI Listing
February 2019

KLF4 Regulates Corneal Epithelial Cell Cycle Progression by Suppressing Canonical TGF-β Signaling and Upregulating CDK Inhibitors P16 and P27.

Invest Ophthalmol Vis Sci 2019 Feb;60(2):731-740

Department of Ophthalmology, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania, United States.

Purpose: Krüppel-like factor 4 (KLF4) promotes corneal epithelial (CE) cell fate while suppressing mesenchymal properties. TGF-β plays a crucial role in cell differentiation and development, and if dysregulated, it induces epithelial-mesenchymal transition (EMT). As KLF4 and TGF-β regulate each other in a context-dependent manner, we evaluated the role of the crosstalk between KLF4 and TGF-β-signaling in CE homeostasis. Read More

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http://dx.doi.org/10.1167/iovs.18-26423DOI Listing
February 2019

Continuous intracerebroventricular injection of Porphyromonas gingivalis lipopolysaccharide induces systemic organ dysfunction in a mouse model of Alzheimer's disease.

Exp Gerontol 2019 Feb 17. Epub 2019 Feb 17.

Department of Pharmacology and Molecular Therapeutics, Graduate School of Medical Sciences, Kumamoto University, Japan.

Systemic organ dysfunction is one of the important issues for the patients with Alzheimer's disease (AD) and their caregivers. Recent evidences suggest that periodontitis is a possible risk factor for progression of AD and lipopolysaccharide derived from Porphyromonas gingivalis (Pg-LPS) which is a major periodontopathic bacteria induces cognitive impairment in mice. However, the precise relationships between the brain exposure of Pg-LPS and systemic organ dysfunction in AD patients are still undetermined. Read More

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http://dx.doi.org/10.1016/j.exger.2019.02.007DOI Listing
February 2019

An industry perspective: a streamlined screening strategy using alternative models for chemical assessment of developmental neurotoxicity.

Neurotoxicology 2019 Feb 17. Epub 2019 Feb 17.

Toxicology & Environmental Research and Consulting, The Dow Chemical Company, Midland, Michigan. Electronic address:

Developmental neurotoxicity (DNT) is an important endpoint for the safety assessment of chemicals. However, the current in vivo animal model for DNT assessment is resource-intensive and may not fully capture all mechanisms that may be relevant to DNT in humans. As a result, there is a growing need for more reliable, time- and cost-efficient approaches for DNT evaluation. Read More

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http://dx.doi.org/10.1016/j.neuro.2019.02.010DOI Listing
February 2019

Molecular Mechanisms of Plant Regeneration.

Annu Rev Plant Biol 2019 Feb 20. Epub 2019 Feb 20.

RIKEN Center for Sustainable Resource Science, Yokohama, Kanagawa 230-0045, Japan; email: , , , , , ,

Plants reprogram somatic cells following injury and regenerate new tissues and organs. Upon perception of inductive cues, somatic cells often dedifferentiate, proliferate, and acquire new fates to repair damaged tissues or develop new organs from wound sites. Wound stress activates transcriptional cascades to promote cell fate reprogramming and initiate new developmental programs. Read More

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http://dx.doi.org/10.1146/annurev-arplant-050718-100434DOI Listing
February 2019

A Molecular View of Plant Local Adaptation: Incorporating Stress-Response Networks.

Annu Rev Plant Biol 2019 Feb 20. Epub 2019 Feb 20.

Department of Plant Biology, Michigan State University, East Lansing, Michigan 48824, USA; email:

Ecological specialization in plants occurs primarily through local adaptation to different environments. Local adaptation is widely thought to result in costly fitness trade-offs that result in maladaptation to alternative environments. However, recent studies suggest that such trade-offs are not universal. Read More

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http://dx.doi.org/10.1146/annurev-arplant-050718-100114DOI Listing
February 2019

Chloroplast Lipids and Their Biosynthesis.

Annu Rev Plant Biol 2019 Feb 20. Epub 2019 Feb 20.

Institute of Molecular Physiology and Biotechnology of Plants (IMBIO), University of Bonn, 53115 Bonn, Germany; email:

Chloroplasts contain high amounts of monogalactosyldiacylglycerol (MGDG) and digalactosyldiacylglycerol (DGDG) and low levels of the anionic lipids sulfoquinovosyldiacylglycerol (SQDG), phosphatidylglycerol (PG), and glucuronosyldiacylglycerol (GlcADG). The mostly extraplastidial lipid phosphatidylcholine is found only in the outer envelope. Chloroplasts are the major site for fatty acid synthesis. Read More

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http://dx.doi.org/10.1146/annurev-arplant-050718-100202DOI Listing
February 2019

The Scope, Functions, and Dynamics of Posttranslational Protein Modifications.

Annu Rev Plant Biol 2019 Feb 20. Epub 2019 Feb 20.

Systembiologie der Pflanze, Universität Hohenheim, 70599 Stuttgart, Germany; email:

Assessing posttranslational modification (PTM) patterns within protein molecules and reading their functional implications present grand challenges for plant biology. We combine four perspectives on PTMs and their roles by considering five classes of PTMs as examples of the broader context of PTMs. These include modifications of the N terminus, glycosylation, phosphorylation, oxidation, and N-terminal and protein modifiers linked to protein degradation. Read More

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http://dx.doi.org/10.1146/annurev-arplant-050718-100211DOI Listing
February 2019

Molecular Interactions Between Plants and Insect Herbivores.

Annu Rev Plant Biol 2019 Feb 20. Epub 2019 Feb 20.

Department of Plant Molecular Biology, University of Lausanne, 1015 Lausanne, Switzerland; email:

Diverse molecular processes regulate the interactions between plants and insect herbivores. Here, we review genes and proteins that are involved in plant-herbivore interactions and discuss how their discovery has structured the current standard model of plant-herbivore interactions. Plants perceive damage-associated and, possibly, herbivore-associated molecular patterns via receptors that activate early signaling components such as Ca, reactive oxygen species, and MAP kinases. Read More

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http://dx.doi.org/10.1146/annurev-arplant-050718-095910DOI Listing
February 2019

Membrane Electroporation and Electropermeabilization: Mechanisms and Models.

Annu Rev Biophys 2019 Feb 20. Epub 2019 Feb 20.

Faculty of Electrical Engineering, University of Ljubljana , SI-1000 Ljubljana, Slovenia; email: ,

Exposure of biological cells to high-voltage, short-duration electric pulses causes a transient increase in their plasma membrane permeability, allowing transmembrane transport of otherwise impermeant molecules. In recent years, large steps were made in the understanding of underlying events. Formation of aqueous pores in the lipid bilayer is now a widely recognized mechanism, but evidence is growing that changes to individual membrane lipids and proteins also contribute, substantiating the need for terminological distinction between electroporation and electropermeabilization. Read More

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http://dx.doi.org/10.1146/annurev-biophys-052118-115451DOI Listing
February 2019

Split Green Fluorescent Proteins: Scope, Limitations, and Outlook.

Annu Rev Biophys 2019 Feb 20. Epub 2019 Feb 20.

Department of Chemistry, Stanford University, Stanford, California 94305, USA; email: ,

Many proteins can be split into fragments that spontaneously reassemble, without covalent linkage, into a functional protein. For split green fluorescent proteins (GFPs), fragment reassembly leads to a fluorescent readout, which has been widely used to investigate protein-protein interactions. We review the scope and limitations of this approach as well as other diverse applications of split GFPs as versatile sensors, molecular glues, optogenetic tools, and platforms for photophysical studies. Read More

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http://dx.doi.org/10.1146/annurev-biophys-051013-022846DOI Listing
February 2019

How Good Can Single-Particle Cryo-EM Become? What Remains Before It Approaches Its Physical Limits?

Authors:
Robert M Glaeser

Annu Rev Biophys 2019 Feb 20. Epub 2019 Feb 20.

Molecular Biophysics and Integrated Bioimaging Division, Lawrence Berkeley National Laboratory, University of California, Berkeley, California 94720, USA; email:

Impressive though the achievements of single-particle cryo-electron microscopy are today, a substantial gap still remains between what is currently accomplished and what is theoretically possible. As is reviewed here, twofold or more improvements are possible as regards ( a) the detective quantum efficiency of cameras at high resolution, ( b) converting phase modulations to intensity modulations in the image, and ( c) recovering the full amount of high-resolution signal in the presence of beam-induced motion of the specimen. In addition, potential for improvement is reviewed for other topics such as optimal choice of electron energy, use of aberration correctors, and quantum metrology. Read More

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http://dx.doi.org/10.1146/annurev-biophys-070317-032828DOI Listing
February 2019

Maspin binds to cardiolipin in mitochondria and triggers apoptosis.

FASEB J 2019 Feb 20:fj201802182R. Epub 2019 Feb 20.

Department of Biochemistry and Molecular Genetics, Feinberg School of Medicine, Northwestern University, Chicago, Illinois, USA.

A central question in cell biology is how cells respond to stress signals and biochemically regulate apoptosis. One critical pathway involves the change of mitochondrial function and release of cytochrome c to initiate apoptosis. In response to apoptotic stimuli, we found that maspin-a noninhibitory member of the serine protease inhibitor superfamily-translocates from the cytosol to mitochondria and binds to cardiolipin in the inner mitochondrial membrane. Read More

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http://dx.doi.org/10.1096/fj.201802182RDOI Listing
February 2019

Kinetics of Drug Binding and Residence Time.

Annu Rev Phys Chem 2019 Feb 20. Epub 2019 Feb 20.

Department of Pharmacy and Biotechnology, University of Bologna, I-40126 Bologna, Italy; email:

The kinetics of drug binding and unbinding is assuming an increasingly crucial role in the long, costly process of bringing a new medicine to patients. For example, the time a drug spends in contact with its biological target is known as residence time (the inverse of the kinetic constant of the drug-target unbinding, 1/ k). Recent reports suggest that residence time could predict drug efficacy in vivo, perhaps even more effectively than conventional thermodynamic parameters (free energy, enthalpy, entropy). Read More

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http://dx.doi.org/10.1146/annurev-physchem-042018-052340DOI Listing
February 2019

Development and Applications of Bioluminescent and Chemiluminescent Reporters and Biosensors.

Annu Rev Anal Chem (Palo Alto Calif) 2019 Feb 20. Epub 2019 Feb 20.

Center for Membrane and Cell Physiology, Department of Molecular Physiology and Biological Physics, and Department of Chemistry, University of Virginia, Charlottesville, Virginia 22908, USA; email:

Although fluorescent reporters and biosensors have become indispensable tools in biological and biomedical fields, fluorescence measurements require external excitation light, thereby limiting their use in thick tissues and live animals. Bioluminescent reporters and biosensors may potentially overcome this hurdle because they use enzyme-catalyzed exothermic biochemical reactions to generate excited-state emitters. This review first introduces the development of bioluminescent reporters, and next, their applications in sensing biological changes in vitro and in vivo as biosensors. Read More

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http://dx.doi.org/10.1146/annurev-anchem-061318-115027DOI Listing
February 2019

Laser Desorption Combined with Laser Postionization for Mass Spectrometry.

Annu Rev Anal Chem (Palo Alto Calif) 2019 Feb 20. Epub 2019 Feb 20.

Department of Chemistry, University of Illinois at Chicago, Chicago, Illinois 60607, USA; email:

Lasers with pulse lengths from nanoseconds to femtoseconds and wavelengths from the mid-infrared to extreme ultraviolet (UV) have been used for desorption or ablation in mass spectrometry. Such laser sampling can often benefit from the addition of a second laser for postionization of neutrals. The advantages offered by laser postionization include the ability to forego matrix application, high lateral resolution, decoupling of ionization from desorption, improved analysis of electrically insulating samples, and potential for high sensitivity and depth profiling while minimizing differential detection. Read More

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http://dx.doi.org/10.1146/annurev-anchem-061318-115447DOI Listing
February 2019

High Throughput Complementary Analysis and Quantitation of Metabolites by MALDI- and Silicon Nanopost Array-Laser Desorption Ionization Mass Spectrometry.

Anal Chem 2019 Feb 20. Epub 2019 Feb 20.

Silicon nanopost arrays (NAPA) have been shown to be effective substrates for laser desorption ionization-mass spectrometry (LDI-MS), and have been used to analyze a variety of samples including peptides, metabolites, drugs, explosives, and intact cells, as well as to image lipids and metabolites in tissue sections. However, no direct comparison has yet been conducted between NAPA-MS and the most commonly-used LDI-MS technique, matrix-assisted laser desorption ionization (MALDI)-MS. In this work, we compare the utility of NAPA-MS to that of MALDI-MS using two common matrices for the analysis of metabolites in cellular extracts and human urine. Read More

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http://dx.doi.org/10.1021/acs.analchem.8b05074DOI Listing
February 2019

Crystal Structure and Conformational Dynamics of Pyrococcus furiosus Prolyl Oligopeptidase.

Biochemistry 2019 Feb 20. Epub 2019 Feb 20.

Enzymes in the prolyl oligopeptidase family possess unique structures and substrate specificities that are important for their biological activity and for potential biocatalytic applications. The crystal structures of Pyrococcus furiosus (Pfu) prolyl oligopeptidases (POP) and the corresponding S477C mutant were solved to 1.9 and 2. Read More

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http://dx.doi.org/10.1021/acs.biochem.9b00031DOI Listing
February 2019

Cellular origin of microRNA-371a-3p in healthy males based on systematic urogenital tract tissue evaluation.

Andrology 2019 Feb 20. Epub 2019 Feb 20.

Pathology (LEPO), Erasmus MC Cancer Institute, University Medical Center, Rotterdam, The Netherlands.

Background: The microRNA-371a-3p (miR-371a-3p) has been reported to be an informative liquid biopsy (serum and plasma) molecular biomarker for both diagnosis and follow-up of patients with a malignant (testicular) germ cell tumor ((T)GCT). It is expressed in all histological cancer elements, with the exception of mature teratoma. However, normal testis, semen, and serum of males with a disrupted testicular integrity without a TGCT may contain miR-371a-3p levels above threshold, of which the cellular origin is unknown. Read More

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http://dx.doi.org/10.1111/andr.12595DOI Listing
February 2019

Strongly Correlated Aromatic Molecular Conductor.

Small 2019 Feb 20:e1900299. Epub 2019 Feb 20.

Department of Mechanical and Aerospace Engineering, Research and Education in Energy, Environment & Water (RENEW) Institute, University at Buffalo, The State University of New York, Buffalo, NY, 14260, USA.

Strongly correlated electronic molecules open the way for strong coupling between charge, spin, and lattice degrees of freedom to enable interdisciplinary fields, such as molecular electronic switches and plasmonics, spintronics, information storage, and superconducting circuits. However, despite exciting computational predictions and promising advantages to prepare flexible geometries, the electron correlation effect in molecules has been elusive. Here, the electron correlation effects of molecular plasmonic films are reported to uncover their coupling of charge, spin, lattice, and orbital for the switchable metal-to-insulator transition under external stimuli, at which the simultaneous transition occurs from the paramagnetic, electrical, and thermal conducting state to the diamagnetic, electrical, and thermal insulating state. Read More

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http://dx.doi.org/10.1002/smll.201900299DOI Listing
February 2019

Influence of NKG2C gene deletion and CCR5Δ32 in Pre-eclampsia-Approaching the effect of innate immune gene variants in pregnancy.

Int J Immunogenet 2019 Feb 20. Epub 2019 Feb 20.

Laboratório de Imunobiologia e Imunogenética, Programa de Pós-Graduação em Genética e Biologia Molecular, Departamento de Genética, Universidade Federal do Rio Grande do Sul - UFRGS, Porto Alegre, RS, Brazil.

Pre-eclampsia (PE) is a hypertensive disorder that affects an important number of pregnant women worldwide. The exact causes of PE remain poorly understood. However, inflammation and deregulation of innate immune cells, such as natural killer (NK) cells, contribute to PE pathogenesis. Read More

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http://dx.doi.org/10.1111/iji.12416DOI Listing
February 2019

Concurrent Proteomic Fingerprinting and Molecular Analysis Of Cyathostomins.

Proteomics 2019 Feb 20:e1800290. Epub 2019 Feb 20.

Institute for Parasitology and Tropical Veterinary Medicine, Department of Veterinary Medicine, Freie Universität Berlin, Germany.

Rapid, cost-effective, efficient and reliable helminth species identification is of considerable importance to understand host-parasite interactions, clinical disease and drug resistance. Cyathostomins (Nematoda: Strongylidae) are considered to be the most important equine parasites, yet research on this group has been hampered by the large number of 50 morphologically differentiated species, their occurrence in mixed infections with up to 15 species and the difficulties associated with conventional identification methods. Here, MALDI-TOF MS, previously successfully applied to identify numerous organisms, was evaluated and compared with conventional and molecular genetic approaches. Read More

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http://dx.doi.org/10.1002/pmic.201800290DOI Listing
February 2019

Genetic association of DNMT variants can play a critical role in defining the methylation patterns in autism.

IUBMB Life 2019 Feb 20. Epub 2019 Feb 20.

Human Molecular Genetics Laboratory, Rajiv Gandhi Centre for Biotechnology, Trivandrum, Kerala, India.

Autism spectrum disorder (ASD) is a complex neurodevelopmental disorder with impairments in social communication, restricted, repetitive and stereotyped behaviors. Both genetic and environmental factors are known to contribute toward pathophysiology of Autism. Environmental influences on gene expression can be mediated by methylation patterns which are established and maintained by DNA methyltransferases. Read More

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http://dx.doi.org/10.1002/iub.2021DOI Listing
February 2019

pH-Responsive Pillar[6]arene-based Water-Soluble Supramolecular Hexagonal Boxes.

Angew Chem Int Ed Engl 2019 Feb 20. Epub 2019 Feb 20.

Tel Aviv University, School of Chemistry, Ramat Aviv, 69978, Tel Aviv, ISRAEL.

We describe the preparation of the first water-soluble pH-responsive supramolecular hexagonal boxes (SHBs) based on multiple charge-assisted hydrogen bonds between peramino-pillar[6]arenes 2 with the molecular "lid" mellitic acid (1a). The interaction between 2 and 1a, as well as the other "lids" pyromellitic and trimesic acids (1b and 1c, respecively) were studied by a combination of experimental and computational methods. Interestingly, the addition of 1a to the complexes of the protonated form of pillar[6]arene 2, i. Read More

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http://dx.doi.org/10.1002/anie.201900217DOI Listing
February 2019

Electron-Deficient Triborane and Tetraborane Ring Compounds: Synthesis, Structure and Bonding.

Angew Chem Int Ed Engl 2019 Feb 20. Epub 2019 Feb 20.

Ruprecht-Karls-Universität Heidelberg, Institut für Anorganische Chemie, Im Neuenheimer Feld 270, 69120, Heidelberg, GERMANY.

Electron-deficient small boron rings are unique in their formation of sigma- and pi-delocalized electron systems, and the avoidance of "classical" structures in terms of two-center-two-electron (2c,2e) bonds. These rings are tolerant to several skeletal electron numbers, making their redox-chemistry highly interesting. In the past decades, the synthesis of a range of stable compounds with varying electron numbers in the B3 and B4 cores was achieved. Read More

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http://dx.doi.org/10.1002/anie.201900563DOI Listing
February 2019

Second-Sphere Biomimetic Multipoint Hydrogen-Bonding Patterns to Boost CO Reduction of Iron Porphyrins.

Angew Chem Int Ed Engl 2019 Feb 20. Epub 2019 Feb 20.

Institut de Biologie Intégrative de la Cellule (I2BC), Institut des Sciences du Vivant Frédéric-Joliot, CEA Saclay, 91191, Gif-sur-Yvette, France.

Inspired by nature's orchestra of chemical subtleties to activate and reduce CO , we have developed a family of iron porphyrin derivatives in to which we have introduced urea groups functioning as multipoint hydrogen-bonding pillars on the periphery of the porphyrinic ring. This structure closely resembles the hydrogen-bond stabilization scheme of the carbon dioxide (CO ) adduct in the carbon monoxide dehydrogenase (CODH). We found that such changes to the second coordination sphere significantly lowered the overpotential for CO reduction in this family of molecular catalysts and importantly increased the CO binding rate while maintaining high turnover frequency (TOF) and selectivity. Read More

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http://dx.doi.org/10.1002/anie.201814339DOI Listing
February 2019

Polymer Dispersity Control via Organocatalyzed Living Radical Polymerization.

Angew Chem Int Ed Engl 2019 Feb 20. Epub 2019 Feb 20.

Nanyang Technological University, School of Physical and Mathematical Sciences, 21 Nanyang Link, 637371, SINGAPORE.

Molecular weight distribution of polymer, termed dispersity (Đ), is a fundamental key parameter for determining polymer material properties. This paper reports a novel approach for controlling Đ, exploiting a temperature-selective radical generation in organocatalyzed living radical polymerization. The polymers with tailored Đ were synthesized in a batch system without the assistance of an external pump. Read More

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http://dx.doi.org/10.1002/anie.201814573DOI Listing
February 2019

HspBP1 and anti-HspBP1 levels in the serum of HIV-infected individuals are associated to the disease progression.

J Appl Microbiol 2019 Feb 20. Epub 2019 Feb 20.

Laboratory of Cellular and Molecular Immunology - Federal University of Health Sciences of Porto Alegre, Av. Sarmento Leite, 245, Centro Historico, 90050-170, Porto Alegre, RS, Brazil.

Aims: The objective of this research was to quantify the levels of circulating HspBP1 and anti-HspBP1 IgG in HIV-infected individuals and to correlate them with CD4 T cell counts and viral load, as well as to determine the kinetics of those proteins during acute phase.

Methods And Results: Sixty serum samples from HIV-positive outpatients, thirty with high viral load and thirty with low viral load were analyzed. The HspBP1and anti-HspBP1 were quantified by ELISA. Read More

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http://dx.doi.org/10.1111/jam.14230DOI Listing
February 2019

Creating Cell and Animal Models of Human Disease by Genome Editing Using CRISPR/Cas9.

J Gene Med 2019 Feb 20:e3082. Epub 2019 Feb 20.

Department of Genetics, Shiraz University of Medical Science, Shiraz, Iran.

Aims: Set of unique sequences in bacterial genomes, responsible for protecting bacteria against bacteriophages, have been recently used for genetic manipulation of specific points in the genome. These system comprises of one RNA component and one enzyme component, known as CRISPR and Cas9, respectively. The present review focuses on the applications of the CRISPR/Cas9 technology in the development of cellular and animal models of human disease. Read More

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http://dx.doi.org/10.1002/jgm.3082DOI Listing
February 2019

Coordinating Tissue Regeneration through TGF-β Activated Kinase 1 (TAK1) In-activation and Re-activation.

Stem Cells 2019 Feb 20. Epub 2019 Feb 20.

Department of Surgery, University of Michigan, Ann Arbor, Michigan.

Aberrant wound healing presents as inappropriate or insufficient tissue formation. Using a model of musculoskeletal injury, we demonstrate that loss of TGF-β activated kinase 1 (TAK1) signaling reduces inappropriate tissue formation (heterotopic ossification) through reduced cellular differentiation. Upon identifying increased proliferation with loss of TAK1 signaling, we considered a regenerative approach to address insufficient tissue production through coordinated inactivation of TAK1 to promote cellular proliferation, followed by re-activation to elicit differentiation and extracellular matrix (ECM) production. Read More

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http://dx.doi.org/10.1002/stem.2991DOI Listing
February 2019

Comprehensive mass spectrometry-guided plant specialized metabolite phenotyping reveals metabolic diversity in the cosmopolitan plant family Rhamnaceae.

Plant J 2019 Feb 20. Epub 2019 Feb 20.

Collaborative Mass Spectrometry Innovation Center, Skaggs School of Pharmacy and Pharmaceutical Sciences, University of California, San Diego, La Jolla, California, 92093, United States.

Plants produce a myriad of specialized metabolites to overcome their sessile habit and combat biotic as well as abiotic stresses. Evolution has shaped specialized metabolite diversity, which drives many other aspects of plant biodiversity. However, until recently, large-scale studies investigating specialized metabolite diversity in an evolutionary context have been limited by the impossibility to identify chemical structures of hundreds to thousands of compounds in a time-feasible manner. Read More

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http://dx.doi.org/10.1111/tpj.14292DOI Listing
February 2019

Maternal Transient Receptor Potential Vanilloid 6 (Trpv6) Is Involved In Offspring Bone Development.

J Bone Miner Res 2019 Feb 20:e3646. Epub 2019 Feb 20.

Pharmacology and Toxicology, Center for Molecular Signaling (PZMS), Saarland University School of Medicine, Homburg, Germany.

Embryonic growth and bone development depend on placental Ca transport across the feto-maternal barrier to supply minerals to the fetus. The individual factors and cellular mechanisms that regulate placental Ca transfer, however, are only beginning to emerge. We find that the Ca -selective transient receptor potential vanilloid 6 (TRPV6) channel is expressed in trophoblasts of the fetal labyrinth, in the yolk sac, and in the maternal part of the placenta. Read More

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http://dx.doi.org/10.1002/jbmr.3646DOI Listing
February 2019

New Organic Electrode Materials for Ultrafast Electrochemical Energy Storage.

Adv Mater 2019 Feb 20:e1806599. Epub 2019 Feb 20.

Helmholtz Institute Ulm, 89081, Ulm, Germany.

Organic materials are both environmentally and economically attractive as potential electrode candidates. This Research News reports on a new class of stable and electrically conductive organic electrodes based on metal porphyrins with functional groups that are capable of electrochemical polymerization, rendering the materials promising for electrochemical applications. Their structural flexibility and the unique highly conjugated macrocyclic structure allows the produced organic electrodes to act as both cathode and anode materials giving access to fast charging as well as high cycling stability. Read More

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http://dx.doi.org/10.1002/adma.201806599DOI Listing
February 2019

mHDFS-HoF: A generalized multilevel homodesmotic fragment-separation reaction based program for heat-of-formation calculation for acyclic hydrocarbons.

J Comput Chem 2019 Feb 20. Epub 2019 Feb 20.

Chemical Engineering Department, International University, VNU-HCM, Vietnam.

Based on our modified classification of elemental species, a framework for automatic generation of multilevel Homodesmotic fragment-separation (mHDFS) reactions for chemical species was proposed. Combined the mHDFS framework with a database of heat of formation (HoF) and the calculated electronic structure data for the elemental mHD species, the mHDFS-HoF program was constructed in C/C++ language to calculate heat of formation for a species of interest on-the-fly. Using the electronic structure data calculated at CBS-QB3 level of theory for the elemental mHD species, applications and robustness of the code were discussed with several acyclic hydrocarbon systems including neutral and radical species. Read More

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http://dx.doi.org/10.1002/jcc.25790DOI Listing
February 2019

Bayesian personalized treatment selection strategies that integrate predictive with prognostic determinants.

Biom J 2019 Feb 20. Epub 2019 Feb 20.

Quantitative Health Sciences and The Taussig Cancer Institute, Cleveland Clinic, Cleveland, OH, USA.

The evolution of "informatics" technologies has the potential to generate massive databases, but the extent to which personalized medicine may be effectuated depends on the extent to which these rich databases may be utilized to advance understanding of the disease molecular profiles and ultimately integrated for treatment selection, necessitating robust methodology for dimension reduction. Yet, statistical methods proposed to address challenges arising with the high-dimensionality of omics-type data predominately rely on linear models and emphasize associations deriving from prognostic biomarkers. Existing methods are often limited for discovering predictive biomarkers that interact with treatment and fail to elucidate the predictive power of their resultant selection rules. Read More

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http://dx.doi.org/10.1002/bimj.201700323DOI Listing
February 2019

The plastidial metabolite 2-C-methyl-D-erythritol-2,4-cyclodiphosphate modulates defense responses against aphids.

Plant Cell Environ 2019 Feb 20. Epub 2019 Feb 20.

Laboratory of Entomology, Wageningen University, PO Box 16, 6700AA, Wageningen, The Netherlands.

Feeding by insect herbivores such as caterpillars and aphids induces plant resistance mechanisms that are mediated by the phytohormones jasmonic acid (JA) and salicylic acid (SA). These phytohormonal pathways often crosstalk. Besides phytohormones, methyl-D-erythriol-2,4-cyclodiphosphate (MEcPP), the penultimate metabolite in the methyl-D-erythritol-4-phosphate (MEP) pathway, has been speculated to regulate transcription of nuclear genes in response to biotic stressors such as aphids. Read More

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http://dx.doi.org/10.1111/pce.13538DOI Listing
February 2019

Human papillomavirus type 8 oncoproteins E6 and E7 cooperate in downregulation of the cellular checkpoint kinase-1.

Int J Cancer 2019 Feb 20. Epub 2019 Feb 20.

Institute of Virology, University of Cologne, Faculty of Medicine and University Hospital of Cologne, Fürst-Pückler-Str. 56, 50935 Cologne, Germany.

Human papillomavirus 8 (HPV8) is associated with the development of squamous cell carcinomas (SCC) of the skin. HPV-infected keratinocytes are able to over-ride normal checkpoint control mechanisms and sustain cell cycle activity, allowing for synthesis of cellular proteins necessary for viral genome amplification. To study how HPV8 may disrupt cell-cycle control, we analysed the impact of HPV8 early gene expression on one of the key regulators of cell cycle and DNA damage response, checkpoint kinase-1 (CHK1). Read More

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http://dx.doi.org/10.1002/ijc.32223DOI Listing
February 2019

NK007 helps in mitigating paclitaxel resistance through p38MAPK activation and HK2 degradation in ovarian cancer.

J Cell Physiol 2019 Feb 20. Epub 2019 Feb 20.

The First Affiliated Hospital, Biomedical Translational Research Institute and School of Pharmacy, Jinan University, Guangzhou, China.

Ovarian cancer resistance to available medicines is a huge challenge in dire need of a solution, which makes its recurrence and mortality rate further exacerbated. A promising approach to overcome chemoresistance is drug screening from natural products. Here, we report that NK007, a (±)-tylophorine malate isolated from the Asclepiadaceae family, selectively inhibited the proliferation of A2780 and A2780 (Taxol) cells and migration of paclitaxel-sensitive and -resistant ovarian cancer cells. Read More

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http://dx.doi.org/10.1002/jcp.28278DOI Listing
February 2019

In vivo prediction of temporomandibular joint disc thickness and position changes for different jaw positions.

J Anat 2019 Feb 20. Epub 2019 Feb 20.

Department of Computer Science, University of Saskatchewan, Saskatoon, SK, Canada.

Temporomandibular joint disorders (TMD) are common dysfunctions of the masticatory region and are often linked to dislocation or changes of the temporomandibular joint (TMJ) disc. Magnetic resonance imaging (MRI) is the gold standard for TMJ imaging but standard clinical sequences do not deliver a sufficient resolution and contrast for the creation of detailed meshes of the TMJ disc. Additionally, bony structures cannot be captured appropriately using standard MRI sequences due to their low signal intensity. Read More

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http://dx.doi.org/10.1111/joa.12951DOI Listing
February 2019
2.097 Impact Factor

Functional Genetic Variation in the Anti-Müllerian Hormone Pathway in Women with Polycystic Ovary Syndrome.

J Clin Endocrinol Metab 2019 Feb 20. Epub 2019 Feb 20.

Division of Endocrinology, Metabolism, and Molecular Medicine, Department of Medicine, Northwestern University Feinberg School of Medicine, Chicago, IL.

Context: PCOS is a highly heritable, common endocrine disorder characterized by hyperandrogenism, irregular menses and polycystic ovaries. PCOS is often accompanied by elevated levels of anti-Müllerian hormone (AMH). AMH inhibits follicle maturation. Read More

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http://dx.doi.org/10.1210/jc.2018-02178DOI Listing
February 2019

Epigenetic age acceleration in adolescence associates with BMI, inflammation and risk score for middle age cardiovascular disease.

J Clin Endocrinol Metab 2019 Feb 20. Epub 2019 Feb 20.

Curtin/UWA Centre for Genetic Origins of Health and Disease, School of Biomedical Sciences, The University of Western Australia.

Background: 'Accelerated ageing', assessed by adult DNA methylation predicts cardiovascular disease (CVD). Adolescent accelerated aging might predict CVD earlier. We investigated whether epigenetic age acceleration (assessed age 17-years) associated with adiposity/CVD-risk measured (ages 17, 20, 22-years), and projected CVD by middle-age. Read More

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http://dx.doi.org/10.1210/jc.2018-02076DOI Listing
February 2019

Stimulated thyroglobulin and Thyroglobulin Reduction Index Predict Excellent Response in Differentiated Thyroid Cancers.

J Clin Endocrinol Metab 2019 Feb 20. Epub 2019 Feb 20.

CHU Clermont-Ferrand, Service d'endocrinologie, diabétologie et maladies métaboliques, Clermont-Ferrand, France.

Introduction: Despite its good prognosis, differentiated thyroid cancer (DTC) is characterized by a high risk of disease persistence and/or recurrence. Estimation of long-term remission (excellent response) thanks to specific parameters could help to personalize the schedule of active surveillance.

Objective: Evaluation of the value of stimulated thyroglobulin and thyroglobulin reduction index (TRI) to predict long-term remission in patients with DTC managed by thyroidectomy and radioactive iodine (RAI) remnant ablation. Read More

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http://dx.doi.org/10.1210/jc.2018-02680DOI Listing
February 2019

DNA Methylation Regulates Corneal Epithelial Wound Healing by Targeting miR-200a and CDKN2B.

Invest Ophthalmol Vis Sci 2019 Feb;60(2):650-660

School of Ophthalmology and Optometry, Eye Hospital, Wenzhou Medical University, Wenzhou, Zhejiang, China.

Purpose: DNA methylation is a key epigenetic modification involved in various biological processes and diseases. Corneal epithelial wound healing (CEWH) is essential for restoring corneal integrity and transparency after injury. However, the role of DNA methylation in CEWH remains elusive. Read More

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http://dx.doi.org/10.1167/iovs.18-25443DOI Listing
February 2019

Functional analysis finds differences on the muscle transcriptome of pigs fed an n-3 PUFA-enriched diet with or without antioxidant supplementations.

PLoS One 2019 20;14(2):e0212449. Epub 2019 Feb 20.

Interdepartmental Centre for Industrial Agrifood Research (CIRI- AGRO), University of Bologna, Cesena, Italy.

Supplementing pig diets with n-3 polyunsaturated fatty acids (n-3 PUFA) may produce meat products with an increased n-3 fatty acid content, and the combined antioxidants addition could prevent lipid oxidation in the feed. However, to date, the effects of these bioactive compounds at the molecular level in porcine skeletal muscle are mostly unknown. This study aimed to analyse changes in the Longissimus thoracis transcriptome of 35 pigs fed three diets supplemented with: linseed (L); linseed, vitamin E and Selenium (LES) or linseed and plant-derived polyphenols (LPE). Read More

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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0212449PLOS
February 2019

Trans ε viniferin decreases amyloid deposits and inflammation in a mouse transgenic Alzheimer model.

PLoS One 2019 20;14(2):e0212663. Epub 2019 Feb 20.

University of Poitiers, EA3808 Neurovascular Unit and Cognitive Disorders, Pôle Biologie Santé, POITIERS, France.

As Alzheimer's disease (AD) induces several cellular and molecular damages, it could be interesting to use multi-target molecules for therapeutics. We previously published that trans ε-viniferin induced the disaggregation of Aβ42 peptide and inhibited the inflammatory response in primary cellular model of AD. Here, effects of this stilbenoid were evaluated in transgenic APPswePS1dE9 mice. Read More

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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0212663PLOS
February 2019
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Molecular features of steroid-binding antidins and their use for assaying serum progesterone.

PLoS One 2019 20;14(2):e0212339. Epub 2019 Feb 20.

Structural Bioinformatics Laboratory, Biochemistry, Faculty of Science and Engineering, Åbo Akademi University, Turku, Finland.

Chicken avidin (Avd) and streptavidin from Streptomyces avidinii are extensively used in bionanotechnology due to their extremely tight binding to biotin (Kd ~ 10-15 M for chicken Avd). We previously reported engineered Avds known as antidins, which have micro- to nanomolar affinities for steroids, non-natural ligands of Avd. Here, we report the 2. Read More

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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0212339PLOS
February 2019

The ovine hepatic mitochondrial proteome: Understanding seasonal weight loss tolerance in two distinct breeds.

PLoS One 2019 20;14(2):e0212580. Epub 2019 Feb 20.

Linking Landscape, Environmental, Agriculture and Food, Instituto Superior de Agronomia, University of Lisbon, Lisbon, Portugal.

Seasonal weight loss (SWL) is a primary constraint for farmers in the Mediterranean and tropics. One cost-effective solution to SWL is utilizing breeds like the Damara sheep that have adapted to deal with nutritional stress. Previous studies concluded that one of the adaptation mechanisms of SWL is a specialized fatty acid metabolism. Read More

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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0212580PLOS
February 2019

RaCaT: An open source and easy to use radiomics calculator tool.

PLoS One 2019 20;14(2):e0212223. Epub 2019 Feb 20.

Department of Nuclear Medicine and Molecular Imaging, University of Groningen, University Medical Center Groningen, Groningen, The Netherlands.

Purpose: The widely known field 'Radiomics' aims to provide an extensive image based phenotyping of e.g. tumors using a wide variety of feature values extracted from medical images. Read More

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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0212223PLOS
February 2019

Metal based donepezil analogues designed to inhibit human acetylcholinesterase for Alzheimer's disease.

PLoS One 2019 20;14(2):e0211935. Epub 2019 Feb 20.

Division of Computer-Aided Drug Design, The Red-Green Research Centre, Dhaka, Bangladesh.

Among neurodegenerative disorders, Alzheimer's disease (AD) is one of the most common disorders showing slow progressive cognitive decline. Targeting acetylcholinesterase (AChE) is one of the major strategies for AD therapeutics, as cholinergic pathways in the cerebral cortex and basal forebrain are compromised. Herein, we report the design of some copper and other metal based donepezil derivatives, employing density functional theory (DFT). Read More

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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0211935PLOS
February 2019
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