9,927 results match your criteria Midgut Volvulus Imaging


Peptide receptor radionuclide therapy for patients with advanced pancreatic neuroendocrine tumors.

Semin Oncol 2018 Aug 24;45(4):236-248. Epub 2018 Oct 24.

Mayo Clinic Cancer Center, Rochester, MN, USA. Electronic address:

Lu-DOTATATE peptide receptor radionuclide therapy (PRRT) is now approved for patients with advanced gastroenteropancreatic neuroendocrine tumors (NET), and it is therefore important to understand the efficacy and safety of PRRT in this patient population. PRRT efficacy and safety outcomes have frequently been summarized for patient populations with gastroenteropancreatic NET, but not specifically in patients with pancreatic NET (panNET). The pivotal phase 3 trial of Lu-DOTATATE PRRT in NET was restricted to patients with a midgut primary site. Read More

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Functional characterization of horizontally transferred 4,5-DOPA extradiol dioxygenase gene in the domestic silkworm, Bombyx mori.

Insect Mol Biol 2018 Dec 10. Epub 2018 Dec 10.

Laboratory of Evolutionary and Functional Genomics, School of Life Sciences, Chongqing University, Chongqing, 401331, China.

4,5-DOPA dioxygenase (DODA) is a crucial enzyme in the biosynthetic pathway of betalain. Previous studies have shown that DODA is present in plants, fungi and bacteria. Using updated data, here, we show that DODA genes (BmDODA) in domestic silkworm (Bombyx mori) and other lepidopteran insects are most likely to be horizontally transferred from fungi. Read More

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December 2018

Prenatal diagnosis of antenatal midgut volvulus: Specific ultrasound features.

Prenat Diagn 2018 Dec 9. Epub 2018 Dec 9.

Department of Pediatric Imaging, CHU Montpellier, Montpellier, France.

Objective: To assess specific, direct, and indirect prenatal ultrasound features in cases of fetal midgut volvulus.

Methods: Retrospective case series of neonatal volvulus, based on postnatal and prenatal imaging findings that occurred from 2006-2017. Prenatal and postnatal signs including the specific "whirlpool sign" were computed. Read More

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December 2018

The Enteric Neural Crest Progressively Loses Capacity to form Enteric Nervous System.

Dev Biol 2018 Dec 7. Epub 2018 Dec 7.

Murdoch Children's Research Institute, Royal Children's Hospital, Parkville 3052, Victoria, Australia.

Cells of the vagal neural crest (NC) form most of the enteric nervous system (ENS) by a colonising wave in the embryonic gut, with high cell proliferation and differentiation. Enteric neuropathies have an ENS deficit and cell replacement has been suggested as therapy. This would be performed post-natally, which raises the question of whether the ENS cell population retains its initial ENS-forming potential with age. Read More

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December 2018

Identification of midgut membrane proteins from different instars of Helicoverpa armigera (Lepidoptera: Noctuidae) that bind to Cry1Ac toxin.

PLoS One 2018 6;13(12):e0207789. Epub 2018 Dec 6.

Instituto de Biotecnología, Universidad Nacional Autónoma de México, Morelos, Mexico.

Helicoverpa armigera is a polyphagous pest sensitive to Cry1Ac protein from Bacillus thuringiensis (Bt). The susceptibility of the different larval instars of H. armigera to Cry1Ac protoxin showed a significant 45-fold reduction in late instars compared to early instars. Read More

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December 2018

Silver effects on silkworm, Bombyx mori.

J Toxicol Sci 2018 ;43(12):697-709

School of Food and Biological Engineering, Jiangsu University, China.

Silver nanoparticles (Ag-NPs) are known as a noble metal, and owing to their exclusive properties, their use is widespread in consumer products and they are mostly incorporated into food packaging and food contact products. The aim of this work was to evaluate the effects of direct ingestion of Ag-NPs through food to assess their toxicity effects on the growth and development of silkworms at different concentrations (1 mg·L to 100 mg·L), in addition to the examination of the distribution of Ag-NPs in the silkworm body and midgut histopathological analysis. RNA sequencing was performed to investigate the transcriptomic responses to Ag-NPs exposure. Read More

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January 2018
1 Read

The Composition of Midgut Bacteria in Aedes aegypti (Diptera: Culicidae) That Are Naturally Susceptible or Refractory to Dengue Viruses.

J Insect Sci 2018 Nov 1;18(6). Epub 2018 Nov 1.

C2D2 Research Group, Department of Biological Sciences, Simon Fraser University, Burnaby, BC, Canada.

The composition, abundance, and diversity of midgut bacteria in mosquitoes can influence pathogen transmission. We used 16S rRNA microbiome profiling to survey midgut microbial diversity in pooled samples of laboratory colonized dengue-refractory, Cali-MIB, and dengue-susceptible, Cali-S Aedes aegypti (Linnaeus). The 16S rRNA sequences from the sugar-fed midguts of adult females clustered to 63 amplicon sequence variants (ASVs), primarily from Proteobacteria, Firmicutes, Flavobacteria, and Actinobacteria. Read More

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November 2018

The Midgut Muscle Network of Anopheles aquasalis (Culicidae, Anophelinae): Microanatomy and Structural Modification After Blood Meal and Plasmodium vivax (Haemosporida, Plasmodiidae) Infection.

J Med Entomol 2018 Dec 1. Epub 2018 Dec 1.

Fundação de Medicina Tropical Dr. Heitor Vieira Dourado, Avenida Pedro Teixeira, Manaus, PMG, Brazil.

The mosquito midgut is divided into two regions named anterior midgut (AMG) and posterior midgut (PMG). The midgut expands intensely after the blood ingestion to accommodate a large amount of ingested food. To efficiently support the bloodmeal-induced changes, the organization of the visceral muscle fibers has significant adjustments. Read More

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December 2018

The intestinal microbiota of larvae is affected by diet and shows a diverse composition in the different midgut regions.

Appl Environ Microbiol 2018 Nov 30. Epub 2018 Nov 30.

Department of Agricultural Sciences, University of Naples "Federico II", Portici (NA), Italy

The larva of the black soldier fly () has emerged as an efficient system for the bioconversion of organic waste. Although many research efforts are devoted to the optimization of rearing conditions to increase the yield of the bioconversion process, microbiological aspects related to this insect are still neglected. Here we describe the microbiota of the midgut of larvae showing the effect of different diets and midgut regions in shaping microbial load and diversity. Read More

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November 2018
3 Reads

Fiber-associated spirochetes are major agents of hemicellulose degradation in the hindgut of wood-feeding higher termites.

Proc Natl Acad Sci U S A 2018 Nov 30. Epub 2018 Nov 30.

Research Group Insect Gut Microbiology and Symbiosis, Max Planck Institute for Terrestrial Microbiology, 35043 Marburg, Germany.

Symbiotic digestion of lignocellulose in wood-feeding higher termites (family Termitidae) is a two-step process that involves endogenous host cellulases secreted in the midgut and a dense bacterial community in the hindgut compartment. The genomes of the bacterial gut microbiota encode diverse cellulolytic and hemicellulolytic enzymes, but the contributions of host and bacterial symbionts to lignocellulose degradation remain ambiguous. Our previous studies of spp. Read More

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November 2018
2 Reads

Sublethal Effects of Fruit Extract and Its Pure Glycoalkaloids on the Physiology of (Mealworm).

Toxins (Basel) 2018 Dec 1;10(12). Epub 2018 Dec 1.

Department of Animal Physiology and Development, Institute of Experimental Biology, Faculty of Biology, Adam Mickiewicz University in Poznań, ul. Umultowska 89, 61-614 Poznań, Poland.

Background: plants produce glycoalkaloids (GAs) that affect various physiological processes of herbivorous insects and they are being tested as potential alternatives for synthetic pesticides. They cause lethal and sublethal effects. Nevertheless, their mode of action remains unclear. Read More

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December 2018

Molecular interactions between tospoviruses and thrips vectors.

Curr Opin Virol 2018 Dec 27;33:191-197. Epub 2018 Nov 27.

Department of Entomology and Plant Pathology, North Carolina State University, Raleigh, NC, USA.

Thrips-transmitted tospoviruses are an emerging and re-emerging threat to crop production worldwide. Tospoviruses are transstadially transmitted from larval to pupal stages of development, with adults serving as the primary inoculators of plants. A unique feature of the transmission cycle is that adults-while they can acquire virus from plants directly-are competent as vectors only if they acquire virus as larvae. Read More

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December 2018
2 Reads

Identification and Characterization of Three New Cytochrome P450 Genes and the Use of RNA Interference to Evaluate Their Roles in Antioxidant Defense in Fabricius.

Front Physiol 2018 15;9:1608. Epub 2018 Nov 15.

College of Animal Science and Technology, Shandong Agricultural University, Tai'an, China.

Cytochrome P450s play critical roles in maintaining redox homeostasis and protecting organisms from the accumulation of toxic reactive oxygen species (ROS). The biochemical functions of the P450 family have essentially been associated with the metabolism of xenobiotics. Here, we sequenced and characterized three P450 genes, , and , from Fabricius; these genes play a critical role in maintaining biodiversity. Read More

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November 2018
1 Read

Methoprene-Tolerant (Met) Is Indispensable for Larval Metamorphosis and Female Reproduction in the Cotton Bollworm .

Front Physiol 2018 15;9:1601. Epub 2018 Nov 15.

State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, China.

Juvenile hormone (JH) represses larval metamorphosis and induces adult reproduction in insects. Methoprene-tolerant (Met) is identified as an intranuclear receptor that mediates JH actions. In the present study, we characterized a Met from the severe agricultural pest, , namely HaMet. Read More

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November 2018

A toxicological, metabonomic and transcriptional analysis to investigate the property of mulberry 1-deoxynojirimycin against the growth of Samia cynthia ricini.

Pestic Biochem Physiol 2018 Nov 28;152:45-54. Epub 2018 Aug 28.

School of Pharmaceutical Sciences, Wenzhou Medical University, Wenzhou, China. Electronic address:

1-Deoxynojirimycin (DNJ) is a natural d-glucose analogue from mulberry with promising physiological activity in vivo. Up to the present, the antidiabetic effects of DNJ on lowering blood sugar and accelerating lipid metabolism in mammals were broadly reported, but the specific character of DNJ against insects was vastly ignored. In this study, a toxicological test of DNJ againgst eri-silkworm, Samia cynthia ricini was carried out to investigate the potential of DNJ in insect management. Read More

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November 2018
1 Read

Inhibition of JNK signaling in the Asian malaria vector Anopheles stephensi extends mosquito longevity and improves resistance to Plasmodium falciparum infection.

PLoS Pathog 2018 Nov 29;14(11):e1007418. Epub 2018 Nov 29.

Department of Entomology, Plant Pathology and Nematology and Department of Biological Sciences, University of Idaho, Moscow, ID, United States of America.

Malaria is a global health concern caused by infection with Plasmodium parasites. With rising insecticide and drug resistance, there is a critical need to develop novel control strategies, including strategies to block parasite sporogony in key mosquito vector species. MAPK signaling pathways regulated by extracellular signal-regulated kinases (ERKs) and the stress-activated protein kinases (SAPKs) c-Jun N-terminal kinases (JNKs) and p38 MAPKs are highly conserved across eukaryotes, including mosquito vectors of the human malaria parasite Plasmodium falciparum. Read More

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November 2018

The relapsing fever spirochete Borrelia turicatae persists in the highly oxidative environment of its soft-bodied tick vector.

Cell Microbiol 2018 Nov 29:e12987. Epub 2018 Nov 29.

Departments of Pediatrics and Molecular Virology and Microbiology, Baylor College of Medicine, Houston, Texas, United States of America.

The relapsing fever spirochete Borrelia turicatae possesses a complex life cycle in its soft-bodied tick vector, Ornithodoros turicata. Spirochetes enter the tick midgut during a bloodmeal, and during the following weeks spirochetes disseminate throughout O. turicata. Read More

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November 2018
1 Read

Assessing the Efficiency of Molecular Markers for the Species Identification of Gregarines Isolated from the Mealworm and Super Worm Midgut.

Microorganisms 2018 Nov 27;6(4). Epub 2018 Nov 27.

Department of Life Sciences, University of Siena, 53100 Siena, Italy.

Protozoa, of the taxon Gregarinasina, are a heterogeneous group of Apicomplexa that includes ~1600 species. They are parasites of a large variety of both marine and terrestrial invertebrates, mainly annelids, arthropods and mollusks. Unlike coccidians and heamosporidians, gregarines have not proven to have a negative effect on human welfare; thus, they have been poorly investigated. Read More

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November 2018

Vrille is required for larval moulting and metamorphosis of Helicoverpa armigera (Lepidoptera: Noctuidae).

Insect Mol Biol 2018 Nov 28. Epub 2018 Nov 28.

Department of Entomology and, MOA Key Lab of Pest Monitoring and Green Management, College of Plant Protection, China Agricultural University, 100193, Beijing, China.

Vrille (Vri), a basic leucine zipper (bZIP) transcription factor, plays important roles in insect circadian clock regulation, tracheal development, proliferation, flight, and metamorphosis. Here, Helicoverpa armigera was used as a model to investigate the role of Vri in larval moulting and metamorphosis. Sequence analysis results revealed that Helicoverpa armigera Vri (HaVri) shares a high amino acid identity with other Lepidoptera Vri homologues. Read More

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November 2018

An assessment of the ability to ingest and excrete microplastics by filter-feeders: A case study with the Mediterranean mussel.

Environ Pollut 2018 Nov 15;245:600-606. Epub 2018 Nov 15.

MARE - Marine and Environmental Sciences Centre, Departamento de Ciências e Engenharia do Ambiente, Faculdade de Ciências e Tecnologia da Universidade Nova de Lisboa, 2829-516, Caparica, Portugal.

Plastic debris has been recognized as a growing threat to marine biota due to its widespread distribution and possible interactions with marine species. Concerns over the effects of plastic polymers in marine ecosystems is reflected in the high number of toxicological studies, regarding microplastics (<5 mm) and marine fauna. Although several studies reported that organisms ingest and subsequently eliminate microplastics (MP), the potential effects at organ and tissue level remain unclear, especially considering exposure to different microplastic sizes and concentrations. Read More

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November 2018

Parameters of oxidative stress, cholinesterase activity, Cd bioaccumulation in the brain and midgut of Lymantria dispar (Lepidoptera: Lymantriidae) caterpillars from unpolluted and polluted forests.

Chemosphere 2018 Nov 22;218:416-424. Epub 2018 Nov 22.

University of Belgrade, Institute of Biological Research "Siniša Stanković", Department of Insect Physiology and Biochemistry, Despot Stefan Blvd. 142, 11060 Belgrade, Serbia.

Cadmium (Cd) can display a variety of different effects on living organisms. The objectives of the present study were to investigate Cd bioaccumulation and differences in parameters of oxidative stress (activities of the enzymes: superoxide dismutase, catalase, glutathione reductase, ascorbate peroxidase, glutathione S-transferase, and amounts of non-enzymatic free sulfhydryl groups and total glutathione) and cholinesterase activity in larval brain and midgut tissues of the polyphagous forest insect Lymantria dispar collected from unpolluted and polluted oak forests. Fourth instar L. Read More

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November 2018

ALP2 is a functional receptor of Cry1Ac but not Cry2Ab in Helicoverpa zea.

Insect Mol Biol 2018 Nov 26. Epub 2018 Nov 26.

Department of Entomology and BIO5 Institute, University of Arizona, Tucson, AZ, 85721.

Although membrane-bound alkaline phosphatases (ALPs) have been proposed as a receptor for Cry1Ac in a few Lepidopteran speceis, their potential functions as a Cry2Ab receptor are yet to be verified. To determine if ALP2 also serves as a receptor for Cry1Ac and even for Cry2Ab in Helicoverpa zea, we measured the potency of activated Cry1Ac and Cry2Ab against the midgut and fat body cell lines of H. zea and the ovarian cell line of Spodoptera frugiperda (Sf9) expressing H. Read More

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November 2018
3 Reads

The DNA damage response is developmentally regulated in the African trypanosome.

DNA Repair (Amst) 2018 Nov 14. Epub 2018 Nov 14.

The Wellcome Centre for Molecular Parasitology, College of Medical, Veterinary and Life Sciences, Institute of Infection, Immunity and Inflammation, University of Glasgow, Sir Graeme Davies Building, 120 University Place, Glasgow, G12 8TA, UK. Electronic address:

Genomes are affected by a wide range of damage, which has resulted in the evolution of a number of widely conserved DNA repair pathways. Most of these repair reactions have been described in the African trypanosome Trypanosoma brucei, which is a genetically tractable eukaryotic microbe and important human and animal parasite, but little work has considered how the DNA damage response operates throughout the T. brucei life cycle. Read More

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November 2018

Analysis of the gut-specific microbiome from field-captured tsetse flies, and its potential relevance to host trypanosome vector competence.

BMC Microbiol 2018 Nov 23;18(Suppl 1):146. Epub 2018 Nov 23.

Department of Epidemiology of Microbial Diseases, Yale School of Public Health, New Haven, CT, USA.

Background: The tsetse fly (Glossina sp.) midgut is colonized by maternally transmitted and environmentally acquired bacteria. Additionally, the midgut serves as a niche in which pathogenic African trypanosomes reside within infected flies. Read More

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November 2018
1 Read
2.730 Impact Factor

Boundary behaviours of Leishmania mexicana: A hydrodynamic simulation study.

J Theor Biol 2018 Nov 20;462:311-320. Epub 2018 Nov 20.

Wolfson Centre for Mathematical Biology, Mathematical Institute, University of Oxford, Oxford, OX2 6GG, UK. Electronic address:

It is well established that the parasites of the genus Leishmania exhibit complex surface interactions with the sandfly vector midgut epithelium, but no prior study has considered the details of their hydrodynamics. Here, the boundary behaviours of motile Leishmania mexicana promastigotes are explored in a computational study using the boundary element method, with a model flagellar beating pattern that has been identified from digital videomicroscopy. In particular a simple flagellar kinematics is observed and quantified using image processing and mode identification techniques, suggesting a simple mechanical driver for the Leishmania beat. Read More

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November 2018

n. gen., n. sp. (Nematoda: Rhabditida: Thelastomatidae) from Appalachian Polydesmid Millipedes (Polydesmida: Xystodesmidae).

J Nematol 2018 Sep 3;50(2):133-146. Epub 2018 Sep 3.

Department of Entomology and Plant Pathology, University of Tennessee, 370 Plant Biotechnology Building, 2505 E J Chapman Drive, Knoxville, TN 37996-4560 USA.

Stauratostoma shelleyi n. gen., n. Read More

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September 2018
2 Reads

Fine scale differences within the vagal neural crest for enteric nervous system formation.

Dev Biol 2018 Nov 16. Epub 2018 Nov 16.

Murdoch Children's Research Institute, Royal Children's Hospital, Parkville 3052, Victoria, Australia. Electronic address:

The enteric nervous system is mostly derived from vagal neural crest (NC) cells adjacent to somites (s)1-7. We used in ovo focal fluorescent vital dyes and focal electroporation of fluorophore-encoding plasmids in quail embryos to investigate NC cell migration to the foregut initially and later throughout the entire gut. NC cells of different somite-level origins were largely separate until reaching the foregut at about QE2. Read More

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November 2018
3 Reads

Phaseolin ingestion affects vesicular traffic causing oxidative stress in the midgut of Callosobruchus maculatus larvae.

Comp Biochem Physiol B Biochem Mol Biol 2018 Nov 14;228:34-40. Epub 2018 Nov 14.

Departamento de Bioquímica, Centro de Ciências Biológicas, Universidade Federal de Santa Catarina, C.P. 476, Florianópolis 88040-900, Brazil. Electronic address:

It has been reported that phaseolin, the major storage globulin of the common bean (Phaseolus vulgaris), is toxic to Callosobruchus maculatus larvae, an Old World bruchid beetle that is not capable of infesting this New World edible bean. It has also been demonstrated that vicilin, the major storage globulin found in cowpea (Vigna unguiculata) seeds, is absorbed through receptor-mediated endocytosis in the insect midgut. A putative vicilin receptor has been purified and showed high homology to α-tocopherol transfer protein. Read More

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November 2018
2 Reads

Gastric Deserosalization: What Lies Behind Closed Doors.

J Indian Assoc Pediatr Surg 2018 Oct-Dec;23(4):216-218

Department of Anesthesiology, All India Institute of Medical Sciences, New Delhi, India.

Congenital intestinal malrotation has a wide spectrum of presentation varying from incidental detection to recurrent episodes of benign abdominal pain to frank gastrointestinal obstruction, mid-gut volvulus, and bowel gangrene. Herein, we report the first case of congenital intestinal malrotation leading to gastric deserosalization. Intraoperative findings were conspicuous by the presence of midgut volvulus and gastric perforation in the posterior wall. Read More

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November 2018

Laparoscopic Ladd's procedure for malrotation in infants and children is still a controversial approach.

J Pediatr Surg 2018 Oct 28. Epub 2018 Oct 28.

Specialist Neonatal and Paediatric Surgery, Great Ormond Street Hospital, NHS Foundation Trust, London, UK; Stem Cells and Regenerative Medicine Section, UCL Great Ormond Street Institute of Child Health, London, UK. Electronic address:

Background: Open Ladd's procedure is the gold standard for the correction of intestinal malrotation and laparoscopic approach remains controversial. This study aimed to evaluate our experience in laparoscopic management of malrotation.

Methods: Single center retrospective study including patients who underwent a laparoscopic assessment of intestinal malrotation with correction if appropriate between 2007 and 2017. Read More

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October 2018
8 Reads

Litopenaeus vannamei stylicins are constitutively produced by hemocytes and intestinal cells and are differentially modulated upon infections.

Fish Shellfish Immunol 2018 Nov 13;86:82-92. Epub 2018 Nov 13.

Laboratory of Immunology Applied to Aquaculture, Department of Cell Biology, Embryology and Genetics, Federal University of Santa Catarina, 88040-900, Florianópolis, SC, Brazil. Electronic address:

Stylicins are anionic antimicrobial host defense peptides (AAMPs) composed of a proline-rich N-terminal region and a C-terminal portion containing 13 conserved cysteine residues. Here, we have increased our knowledge about these unexplored crustacean AAMPs by the characterization of novel stylicin members in the most cultivated penaeid shrimp, Litopenaeus vannamei. We showed that the L. Read More

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November 2018

Restriction of Zika virus infection and transmission in Aedes aegypti mediated by an insect-specific flavivirus.

Emerg Microbes Infect 2018 Nov 15;7(1):181. Epub 2018 Nov 15.

Division of Vector-Borne Diseases, National Center for Emerging Zoonotic Infectious Diseases, Centers for Disease Control and Prevention, Fort Collins, CO, 80521, USA.

Previous studies demonstrated an insect-specific flavivirus, Nhumirim virus (NHUV), can suppress growth of West Nile virus (WNV) and decrease transmission rates in NHUV/WNV co-inoculated Culex quinquefasciatus. To assess whether NHUV might interfere with transmission of other medically important flaviviruses, the ability of NHUV to suppress viral growth of Zika virus (ZIKV) and dengue-2 virus (DENV-2) was assessed in Aedes albopictus cells. Significant reductions in ZIKV (100,000-fold) and DENV-2 (10,000-fold) were observed in either cells concurrently inoculated with NHUV or pre-inoculated with NHUV. Read More

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November 2018
7 Reads

An Unusual Case of Intestinal Malrotation Causing Duodenal Obstruction by a Looped Appendix.

Am J Case Rep 2018 Nov 15;19:1362-1365. Epub 2018 Nov 15.

Department of General Surgery, Mount Lebanon Hospital, Beirut, Lebanon.

BACKGROUND Bowel obstruction is a mechanical or functional obstruction of the intestines which prevents the normal movement of the products of digestion. Intestinal malrotation is one of the rarest causes of mechanical bowel obstruction. In adults, the incidence rate is 0. Read More

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November 2018
3 Reads

Long-term live imaging of the adult midgut reveals real-time dynamics of division, differentiation and loss.

Elife 2018 11 14;7. Epub 2018 Nov 14.

Department of Molecular and Cellular Physiology, Stanford University School of Medicine, Stanford, United States.

Organ renewal is governed by the dynamics of cell division, differentiation and loss. To study these dynamics in real time, we present a platform for extended live imaging of the adult midgut, a premier genetic model for stem-cell-based organs. A window cut into a living animal allows the midgut to be imaged while intact and physiologically functioning. Read More

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November 2018
8.520 Impact Factor

The insect, Galleria mellonella, is a compatible model for evaluating the toxicology of okadaic acid.

Cell Biol Toxicol 2018 Nov 13. Epub 2018 Nov 13.

Department of Biosciences, College of Science, Swansea University, Swansea, Wales, SA2 8PP, UK.

The polyether toxin, okadaic acid, causes diarrhetic shellfish poisoning in humans. Despite extensive research into its cellular targets using rodent models, we know little about its putative effect(s) on innate immunity. We inoculated larvae of the greater wax moth, Galleria mellonella, with physiologically relevant doses of okadaic acid by direct injection into the haemocoel (body cavity) and/or gavage (force-feeding). Read More

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November 2018
9 Reads

Paraquat-Mediated Oxidative Stress in Mosquitoes Is Regulated by An Endoplasmic Reticulum (ER) Stress Response.

Proteomes 2018 Nov 12;6(4). Epub 2018 Nov 12.

School of Life Science and Bioengineering, Nelson Mandela-African Institution of Science and Technology, Tengeru, Arusha 23302, Tanzania.

Paraquat is a potent superoxide (O₂)-inducing agent that is capable of inducing an oxidative imbalance in the mosquito midgut. This oxidative imbalance can super-stress the malaria parasite, leading to arrested development in the mosquito midgut and reduced transmission. While several studies have explored the effect of paraquat on malaria parasites, a fundamental understanding of the mosquito response to this compound remains unknown. Read More

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November 2018
5 Reads

Polyamidoamine Nanoparticles for the Oral Administration of Antimalarial Drugs.

Pharmaceutics 2018 Nov 10;10(4). Epub 2018 Nov 10.

Barcelona Institute for Global Health (ISGlobal, Hospital Clínic-Universitat de Barcelona), Rosselló 149-153, ES-08036 Barcelona, Spain.

Current strategies for the mass administration of antimalarial drugs demand oral formulations to target the asexual stages in the peripheral bloodstream, whereas recommendations for future interventions stress the importance of also targeting the transmission stages of the parasite as it passes between humans and mosquitoes. Orally administered polyamidoamine (PAA) nanoparticles conjugated to chloroquine reached the blood circulation and cured -infected mice, slightly improving the activity of the free drug and inducing in the animals immunity against malaria. Liquid chromatography with tandem mass spectrometry analysis of affinity chromatography-purified PAA ligands suggested a high adhesiveness of PAAs to proteins, which might be the mechanism responsible for the preferential binding of PAAs to -infected erythrocytes vs. Read More

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November 2018
1 Read

Cloning and Expression Profile of Glyceraldehyde-3-Phosphate Dehydrogenase in Haemaphysalis flava (Acari: Ixodidae).

J Med Entomol 2018 Nov 9. Epub 2018 Nov 9.

College of Veterinary Medicine, Hunan Provincial Key Laboratory of Protein Engineering in Animal Vaccines, Hunan Agricultural University, Changsha, China.

Haemaphysalis flava (Acari: Ixodidae) harbors pathogenic microorganisms and transfers these to hosts during blood feeding. Proteomic analysis in the midgut contents of H. flava detected glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and contig 1683 was retrieved as a GAPDH gene fragment by searching our previous transcriptomic library. Read More

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November 2018
9 Reads

Role of a serine protease gene (AccSp1) from Apis cerana cerana in abiotic stress responses and innate immunity.

Cell Stress Chaperones 2018 Nov 9. Epub 2018 Nov 9.

State Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, Taian, 271018, Shandong, People's Republic of China.

Clip-domain serine proteases (Clip-SPs) mediate innate immunity and embryonic development in insects. However, the function of Clip-SPs in Apis cerana cerana is little known. Here, a Clip-SP gene, AccSp1, was identified. Read More

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November 2018
5 Reads

intestinal stem and progenitor cells are major sources and regulators of homeostatic niche signals.

Proc Natl Acad Sci U S A 2018 Nov 7;115(48):12218-12223. Epub 2018 Nov 7.

Department of Genetics, Harvard Medical School, Boston, MA 02115;

Epithelial homeostasis requires the precise balance of epithelial stem/progenitor proliferation and differentiation. While many signaling pathways that regulate epithelial stem cells have been identified, it is probable that other regulators remain unidentified. Here, we use gene-expression profiling by targeted DamID to identify the stem/progenitor-specific transcription and signaling factors in the midgut. Read More

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November 2018
3 Reads

Inhibition of Sodium-Hydrogen Antiport by Antibodies to NHA1 in Brush Border Membrane Vesicles from Whole Aedes aegypti Larvae.

J Membr Biol 2018 Nov 3. Epub 2018 Nov 3.

Department of Physiology and Functional Genomics, University of Florida, Gainesville, FL, 32610, USA.

The present research report describes Na/H antiport by brush border membrane vesicles isolated from whole larvae of Aedes aegypti (AeBBMVw). Our hypothesis is that acid quenching of acridine orange by AeBBMVw is predominantly mediated by Na/H antiport via the NHA1 component of the AeBBMVw in the absence of amino acids and ATP. AeNHA1 is a Na/H antiporter that has been postulated to exchange Na and H across the apical plasma membrane in posterior midgut of A. Read More

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November 2018
2 Reads
2.460 Impact Factor

Molecular cloning and functional analysis of small heat shock protein 19.1 gene from the Chinese oak silkworm, Antheraea pernyi.

Arch Insect Biochem Physiol 2018 Dec 2;99(4):e21516. Epub 2018 Nov 2.

College of Life Sciences, Anhui Agricultural University, Hefei, China.

Small heat shock proteins (sHSPs) are a class of highly conserved proteins that are ubiquitously found in all types of organisms, from prokaryotes to eukaryotes. In the current study, we identified and characterized the full-length cDNA encoding sHSP 19.1 from the oak silkworm, Antheraea pernyi. Read More

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December 2018
1 Read

RNAi-knockdown of the Locusta migratoria nuclear export factor protein results in insect mortality and alterations in gut microbiome.

Pest Manag Sci 2018 Nov 2. Epub 2018 Nov 2.

Chongqing Engineering Research Center for Fungal Insecticide, Genetic Engineering Research Center, School of Life Sciences, Chongqing University, Chongqing, 400044, China.

Background: The migratory locust Locusta migratoria is one of the most important agricultural pests worldwide. The nuclear export factor 1 (NXF1) protein plays a crucial role in mediating mRNAs transport from the nucleus to the cytoplasm. This study was to evaluate whether NXF1 could be a potential target for RNAi-mediated pest control of L. Read More

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November 2018
1 Read
2.690 Impact Factor

De novo transcriptome assembly of the bamboo snout beetle reveals ability to degrade lignocellulose of bamboo feedstock.

Biotechnol Biofuels 2018 27;11:292. Epub 2018 Oct 27.

1Bamboo Diseases and Pests Control and Resources Development Key Laboratory of Sichuan Province, College of Life Science, Leshan Normal University, No. 778, Riverside Road, Central District, Leshan, 614000 China.

Background: The bamboo weevil , which is considered a pest species, damages bamboo shoots via its piercing-sucking mode of feeding. is well known for its ability to transform bamboo shoot biomass into nutrients and energy for growth, development and reproduction with high specificity and efficacy of bioconversion. Woody bamboo is a perennial grass that is a potential feedstock for lignocellulosic biomass because of its high growth rate and lignocellulose content. Read More

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October 2018
7 Reads

The C-terminal protoxin domain of Bacillus thuringiensis Cry1Ab toxin has a functional role in binding to GPI-anchored receptors in the insect midgut.

J Biol Chem 2018 Nov 1. Epub 2018 Nov 1.

MICROBIOLOGIA MOLECULAR, Instituto De Biotecnologia-UNAM, Mexico.

Bacillus thuringiensis (Bt) Cry toxins are used worldwide for controlling insects. Cry1Ab is produced as a 130 kDa protoxin that is activated by proteolytic removal of an inert 500 amino-acids-long C-terminal region, enabling the activated toxin to bind to insect midgut receptor proteins and leading to its membrane insertion and pore formation. It has been proposed that the C-terminal region is only involved in toxin crystallization, but its role in receptor binding is undefined. Read More

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November 2018
5 Reads

Reoperation after Ladd's procedure in the neonatal period.

Pediatr Surg Int 2018 Oct 31. Epub 2018 Oct 31.

Department of Pediatric Surgery, Children's Hospital of Fudan University, Shanghai, China.

Aim Of The Study: To investigate (1) the indications for reoperation after neonatal Ladd's procedure, (2) the type of reoperation and (3) its outcome.

Methods: We reviewed all neonatal Ladd's procedures in our hospital from 2003 to 2017 and the outcomes of reoperation in these patients.

Main Results: 252 neonates had Ladd's procedure: 59 were laparoscopic (23. Read More

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October 2018
1 Read

Reduced diversity of gut microbiota in two Aedes mosquitoes species in areas of recent invasion.

Sci Rep 2018 Oct 31;8(1):16091. Epub 2018 Oct 31.

Fondazione Edmund Mach, Research and Innovation Centre, San Michele all'Adige, Trento, Italy.

Aedes mosquitoes are considered highly successful global invasive species and vectors of several pathogens of relevance for public health. Their midgut's microbiota can play an important role in affecting not only their vectorial competence but also their fitness, physiology, food digestion, metabolism, immunity and adaptation to new environmental conditions. Using high-throughput sequencing we compared the microbiota of Aedes albopictus collected in Italy with those reported in populations from France and Vietnam. Read More

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October 2018
12 Reads

Influence of carcinoid syndrome on the clinical characteristics and outcomes of patients with gastroenteropancreatic neuroendocrine tumors undergoing operative resection.

Surgery 2018 Oct 27. Epub 2018 Oct 27.

Division of Surgical Oncology, The Ohio State University Comprehensive Cancer Center, Columbus, OH. Electronic address:

Background: The incidence, clinical characteristics, and long-term outcomes of patients with gastroenteropancreatic neuroendrocrine tumors and carcinoid syndrome undergoing operative resection have not been well characterized.

Methods: Patients undergoing resection of primary or metastatic gastroenteropancreatic neuroendrocrine tumors between 2000 and 2016 were identified from an 8-institution collaborative database. Clinicopathologic and postoperative characteristics as well as overall survival and disease-free survival were compared among patients with and without carcinoid syndrome. Read More

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October 2018
1 Read

Control of dengue virus in the midgut of Aedes aegypti by ectopic expression of the dsRNA-binding protein Loqs2.

Nat Microbiol 2018 Dec 29;3(12):1385-1393. Epub 2018 Oct 29.

Department of Biochemistry and Immunology, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte/MG, Brazil.

Dengue virus (DENV) is an arbovirus transmitted to humans by Aedes mosquitoes. In the insect vector, the small interfering RNA (siRNA) pathway is an important antiviral mechanism against DENV. However, it remains unclear when and where the siRNA pathway acts during the virus cycle. Read More

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December 2018
5 Reads

Characterization of Sphingomyelin Phosphodiesterase Expression in Bumblebee (Bombus lantschouensis).

J Insect Sci 2018 Sep 1;18(5). Epub 2018 Sep 1.

Key Laboratory of Insect-Pollinator Biology of the Ministry of Agriculture, Institute of Apicultural Research, Chinese Academy of Agricultural Sciences, Xiangshan, Haidian District, Beijing, China.

Sphingomyelin phosphodiesterase (SMPD) is a hydrolase that plays a major role in metabolic reactions involving sphingomyelin. Here, we describe an analysis of the cDNA sequence and gene structure of SMPD in bumblebee (Bombus lantschouensis). The expression of SMPD in different tissues and at different developmental stages and reproductive statuses was examined by real-time polymerase chain reaction (PCR). Read More

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September 2018
1 Read