1,751 results match your criteria Microsporidiosis


SYSTEMIC ENCEPHALITOZOONOSIS DUE TO ENCEPHALITOZOON CUNICULI STRAIN IV IN A VANCOUVER ISLAND MARMOT ( MARMOTA VANCOUVERENSIS).

J Zoo Wildl Med 2018 Jun;49(2):484-488

  A 2-mo-old Vancouver Island marmot ( Marmota vancouverensis), housed at a quarantined breeding facility, presented for acute obtundation and vestibular ataxia. Physical examination revealed poor growth compared with littermates, poor nutritional condition, and mild dehydration. The animal's condition deteriorated over 24 hr, and it was euthanized following the development of generalized seizures. Read More

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June 2018
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First characterization of a microsporidial triosephosphate isomerase and the biochemical mechanisms of its inactivation to propose a new druggable target.

Sci Rep 2018 Jun 5;8(1):8591. Epub 2018 Jun 5.

Grupo de Investigación en Biomoléculas, Laboratorio de Errores Innatos del Metabolismo y Tamiz, Instituto Nacional de Pediatría, Ciudad de México, 04530, Mexico.

The microsporidia are a large group of intracellular parasites with a broad range of hosts, including humans. Encephalitozoon intestinalis is the second microsporidia species most frequently associated with gastrointestinal disease in humans, especially immunocompromised or immunosuppressed individuals, including children and the elderly. The prevalence reported worldwide in these groups ranges from 0 to 60%. Read More

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Intestinal microsporidiosis in Strasbourg from 2014 to 2016: emergence of an Enterocytozoon bieneusi genotype of Asian origin.

Emerg Microbes Infect 2018 Jun 6;7(1):97. Epub 2018 Jun 6.

Institute of Parasitology and Tropical Diseases, EA 7292, University of Strasbourg, 3 rue Koeberlé, Strasbourg, France.

Microsporidia cause opportunistic infections in highly immunodeficient individuals. Few studies on the epidemiology of these infections have been conducted in France. Between 2014 and 2016, we undertook a study to estimate the prevalence and circulating genotypes of this fungus-related micro-organism among the population of Strasbourg University Hospital. Read More

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June 2018
2 Reads

Seroprevalence Survey for Microsporidia in Common Bottlenose Dolphin ( Tursiops truncatus): Example of A Quantitative Approach Based on Immunoblotting.

J Wildl Dis 2018 May 9. Epub 2018 May 9.

1 University of Miami, Division of Comparative Pathology, Department of Pathology & Laboratory Medicine, Miller School of Medicine, Miami, Florida 33136, USA.

Little is known about microsporidiosis pathogenicity in cetaceans. Here we report seroprevalence of 76% for microsporidia in blood samples from common bottlenose dolphins ( Tursiops truncatus), from animals managed under human care ( n=108) or captured for health assessments ( n=13) and released. Read More

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Molecular surveillance of Vittaforma-like microsporidia by a small-volume procedure in drinking water source in Taiwan: evidence for diverse and emergent pathogens.

Environ Sci Pollut Res Int 2018 Apr 30. Epub 2018 Apr 30.

Graduate Institute of Life Sciences, National Defense Medical Center, No. 161, Section 6, Minquan East Road, Neihu District, Taipei City, 114, Taiwan.

Vittaforma corneae belongs to microsporidia, which include over 1500 species of opportunistic obligate intracellular fungi infecting almost all known animal taxa. Although outbreaks of ocular infections caused by waterborne V. corneae have been reported in recent years, little is known about the occurrence of this pathogen in aquatic environments. Read More

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April 2018
2 Reads

Human-Pathogenic Enterocytozoon bieneusi in Captive Giant Pandas (Ailuropoda melanoleuca) in China.

Sci Rep 2018 Apr 26;8(1):6590. Epub 2018 Apr 26.

The Key Laboratory of Animal Disease and Human Health of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Wenjiang, 611100, China.

Human and animal infections of Enterocytozoon bieneusi (E. bieneusi) have consistently been reported worldwide, garnering public attention; however, the molecular epidemiology of E. bieneusi in the giant panda remains limited. Read More

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April 2018
2 Reads

Case Report: Ocular Microsporidiosis: Case in a Patient Returning from India and Review of the Literature.

Am J Trop Med Hyg 2018 Apr 23. Epub 2018 Apr 23.

INSERM, U995-LIRIC, Fungal Associated Invasive & Inflammatory Diseases, Lille, France.

Microsporidia are protists close to the kingdom of fungi that may cause eye infections. Most cases are reported in Asia and affect both immunocompromised and immunocompetent patients. Here, we report a rare case of microsporidial keratoconjunctivitis in an immunocompetent French patient 3 weeks after returning from India. Read More

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A new microsporidium Percutemincola moriokae gen. nov., sp. nov. from Oscheius tipulae: A novel model of microsporidia-nematode associations.

Parasitology 2018 Apr 17:1-12. Epub 2018 Apr 17.

Department of Immunobiology,School of Pharmacy,Iwate Medical University,Nishitokuta 2-1-1,Yahaba,Shiwa,Iwate,Japan.

Here, we describe a new microsporidium Percutemincola moriokae gen. nov., sp. Read More

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April 2018
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Prevalence and genotypes of Enterocytozoon bieneusi in China.

Acta Trop 2018 Jul 13;183:142-152. Epub 2018 Apr 13.

College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi, 712100, People's Republic of China. Electronic address:

Enterocytozoon bieneusi has been considered as the most frequently diagnosed microsporidian species in humans and various animal species, accounting for more than 90% of the cases of human microsporidiosis. Spores of this pathogen excreted from both symptomatic and asymptomatic hosts into environment also would be an important source of waterborne outbreak of microsporidiosis. Due to limited effective drugs available but with too much side effects to mammals (eg. Read More

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July 2018
1 Read

Evaluation of four commercial DNA extraction kits for the detection of Microsporidia and the importance of pretreatments in DNA isolation.

Acta Parasitol 2018 Jun;63(2):386-392

Department of Parasitology, Faculty of Medicine, Erciyes University, Kayseri, Turkey.

Microsporidia are obligate intracellular parasitic protozoa infecting the wide variety of hosts and are commonly known as a cause of chronic diarrhea particularly in immunocompromised individuals. Molecular-based tests have high sensitivity and specificity in disease diagnosis. However, these tests' performance relies on the isolation of DNA in a good concentration. Read More

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June 2018
2 Reads

Rev Chilena Infectol 2018;35(1):73-74

Universidad de Valparaíso, Chile.

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May 2018
1 Read

Renal Microsporidiosis in Pediatric Bone Marrow Transplant Recipients: A Case Series.

Turk Patoloji Derg 2018 Apr 9. Epub 2018 Apr 9.

Department of Histopathology, Apollo Hospitals, CHENNAI, TAMIL NADU, INDIA.

Microsporidiosis is a rare, but emerging opportunistic infection in solid organ transplant and stem cell transplant recipients. Renal involvement in microsporidiosis is very rarely seen in these recipients. We describe two cases of pediatric renal microsporidiosis, diagnosed on renal biopsies, following bone marrow transplantation presenting as severe acute kidney injury. Read More

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Confirmed microsporidial graft infection in a HIV-negative renal transplant recipient: A case report and review of the literature.

Transpl Infect Dis 2018 Jun 10;20(3):e12888. Epub 2018 Apr 10.

Department of Nephrology, Monash Health, Clayton, VIC, Australia.

Microsporidia are intracellular organisms most commonly known to cause opportunistic infection in patients with human immunodeficiency virus (HIV). There have been several case reports of infection in solid organ and bone marrow transplant recipients. Here, we report a case of a non-HIV-infected renal transplant patient with microsporidiosis of the renal tract associated with acute graft dysfunction. Read More

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Visual loop-mediated isothermal amplification (LAMP) for the rapid diagnosis of Enterocytozoon hepatopenaei (EHP) infection.

Parasitol Res 2018 May 17;117(5):1485-1493. Epub 2018 Mar 17.

ICAR-Central Institute of Brackishwater Aquaculture, #75 Santhome High Road, Raja Annamalai Puram, Chennai, 600028, India.

The emerging microsporidian parasite Enterocytozoon hepatopenaei (EHP), the causative agent of hepatopancreatic microsporidiosis, has been widely reported in shrimp-farming countries. EHP infection can be detected by light microscopy observation of spores (1.7 × 1 μm) in stained hepatopancreas (HP) tissue smears, HP tissue sections, and fecal samples. Read More

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May 2018
8 Reads

Identification, characterization and heparin binding capacity of a spore-wall, virulence protein from the shrimp microsporidian, Enterocytozoon hepatopenaei (EHP).

Parasit Vectors 2018 03 12;11(1):177. Epub 2018 Mar 12.

Department of Biochemistry, Faculty of Science, Mahidol University, Bangkok, Thailand.

Background: The microsporidian Enterocytozoon hepatopenaei (EHP) is a spore-forming, intracellular parasite that causes an economically debilitating disease (hepatopancreatic microsporidiosis or HPM) in cultured shrimp. HPM is characterized by growth retardation and wide size variation that can result in economic loss for shrimp farmers. Currently, the infection mechanism of EHP in shrimp is poorly understood, especially at the level of host-parasite interaction. Read More

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A new scope for orlistat: Effect of approved anti-obesity drug against experimental microsporidiosis.

Med Mycol 2018 Feb 24. Epub 2018 Feb 24.

Department of Medical Parasitology.

As the current therapies for intestinal microsporidiosis are either inconsistent in their efficacies or hampered by several adverse effects, alternative antimicrosporidial agents are being sought. The present study is the first that was designed to evaluate the potency of orlistat, an approved anti-obesity drug, against intestinal microsporidiosis caused by both Enterocytozoon bieneusi and Encephalitozoon intestinalis. Results were assessed through studying fecal and intestinal spore load, intestinal histopathological changes, viability, and infectivity of spores from treated animals. Read More

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February 2018
1 Read

Prevalence, genotypes, and risk factors of Enterocytozoon bieneusi in Asiatic black bear (Ursus thibetanus) in Yunnan Province, Southwestern China.

Parasitol Res 2018 Apr 15;117(4):1139-1145. Epub 2018 Feb 15.

College of Veterinary Medicine, Yunnan Agricultural University, Kunming, Yunnan Province, 650201, People's Republic of China.

Microsporidiosis is an important zoonotic disease, even leading to severe diarrhea. However, no information about prevalence and genotypes of Enterocytozoon bieneusi infection in Asiatic black bears in southwestern China is available. The objectives of the present study were to investigate the prevalence of E. Read More

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April 2018
3 Reads

Wildlife as an environmental reservoir of Enterocytozoon bieneusi (Microsporidia) – analyses of data based on molecular methods

Ann Parasitol 2017;63(4):265-281

Department of Parasitology, Institute of Genetics and Microbiology, Wrocław University, ul. Przybyszewskiego 63, 51-148 Wrocław, Poland

Enterocytozoon bieneusi is the most commonly identified Microsporidia in humans and has also been detected worldwide in a large group of wild living and domestic animals. The identification of E. bieneusi in wildlife has raised the question of the importance of animal reservoirs in the epidemiology of microsporidiosis and the implications of the infection with this pathogen in hosts. Read More

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Enterocytozoon bieneusi genotypes in Tibetan sheep and yaks.

Parasitol Res 2018 Mar 14;117(3):721-727. Epub 2018 Jan 14.

Centers for Disease Control and Prevention, Atlanta, GA, 30329, USA.

Few studies have been conducted on the distribution of Enterocytozoon bieneusi genotypes in Tibetan sheep and yaks, which live outdoors in extreme climate with high altitude. In this study, fecal specimens from 312 Tibetan sheep and 554 yaks in Qinghai, China, were collected and examined for E. bieneusi by PCR-sequence analysis of the ribosomal internal transcribed spacer. Read More

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March 2018
6 Reads

Divergent Cryptosporidium parvum subtype and Enterocytozoon bieneusi genotypes in dromedary camels in Algeria.

Parasitol Res 2018 Mar 6;117(3):905-910. Epub 2018 Jan 6.

Centers for Disease Control and Prevention, 1600 Clifton Road, Atlanta, GA, 30329, USA.

Little information is available on the occurrence of the zoonotic protists Cryptosporidium spp. and none on Enterocytozoon bieneusi in camels. This preliminary study was conducted to examine the identity of Cryptosporidium subtypes and E. Read More

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March 2018
2 Reads

Intestinal microsporidiosis in Iran: infection in immune-compromised and immunocompetent patients.

Curr Med Mycol 2017 Mar;3(1):30-36

Regional Knowledge Hub and WHO Collaborating Centre for HIV Surveillance, Kerman University of Medical Sciences, Kerman, Iran.

Background And Purpose: Gastroenteritis and the clinical profile caused by , an opportunistic pathogen, may be severe in immunocompromised individuals, especially in AIDS patients. Conventionally, it is necessary to detect the small infective spores in stained smears. However, due to the limitations of the microscopy-based methods, several DNA-based methods such as polymerase chain reaction (PCR) have recently been developed to enhance diagnosis sensitivity. Read More

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March 2017
15 Reads

MICROSPORIDIOSIS IN PEPPERMINT SHRIMP (DECAPODA: HIPPOLYTIDAE: LYSMATA SPP.).

J Zoo Wildl Med 2017 Dec;48(4):1223-1229

Microsporidia are a diverse, parasitic phylum closely related to fungi. They infect a broad range of host species and tissues. This report describes microsporidiosis infection in a captive, wild-caught peppermint shrimp ( Lysmata spp. Read More

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December 2017
2 Reads

First detection and genetic characterisation of Enterocytozoon bieneusi in wild deer in Melbourne's water catchments in Australia.

Parasit Vectors 2018 01 3;11(1). Epub 2018 Jan 3.

Department of Veterinary Biosciences, Melbourne Veterinary School, Faculty of Veterinary and Agricultural Sciences, The University of Melbourne, Parkville, VIC, 3010, Australia.

Background: Enterocytozoon bieneusi is reported to be a common microsporidian of humans and animals in various countries. However, E. bieneusi has yet to be recorded in animals in Australia. Read More

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January 2018
4 Reads

Dominance of the Enterocytozoon bieneusi genotype BEB6 in red deer (Cervus elaphus) and Siberian roe deer (Capreolus pygargus) in China and a brief literature review.

Parasite 2017 21;24:54. Epub 2017 Dec 21.

Department of Parasitology, Harbin Medical University, Harbin, Heilongjiang 150081, PR China.

Enterocytozoon bieneusi is the most frequently diagnosed microsporidian species in humans and is also found in a wide range of animals. It is considered to be an important but neglected zoonotic pathogen. With the development of deer bred in captivity, the number of deer has been increasing in recent years in China and there are more people involved in this work. Read More

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December 2017
6 Reads

Molecular diagnosis of human microsporidian infections.

Trans R Soc Trop Med Hyg 2017 Sep;111(9):382-383

Research Service (151), Corporal Michael J. Crescenz Department of Veterans Affairs (VA) Medical Center, University and Woodland Avenues, Philadelphia, PA 19104, USA.

With the transformation of HIV infection from an acutely life-threatening disease into a chronic condition (as a consequence of the development of effective antiretroviral medication), the perceived clinical importance of diagnosing and treating microsporidian infections diminished, at least in industrialized countries. In locales where effective antiretroviral therapy is not available, as well as in patients with immunodeficiency for reasons other than HIV infection (e.g. Read More

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September 2017
2 Reads

Clinical Signs, Diagnosis, and Treatment of Encephalitozoon cuniculi Infection in Rabbits.

Vet Clin North Am Exot Anim Pract 2018 Jan;21(1):69-82

Pet Care Veterinary Hospital, 5201 Virginia Beach Boulevard, Virginia Beach, VA 23462, USA.

Central vestibular dysfunction caused by Encephalitozoon cuniculi frequently mimics the condition of a peripheral disorder. A negative antibody titer rules out E cuniculi as the cause of present clinical signs. Cerebrospinal fluid analysis including polymerase chain reaction is considered an inappropriate diagnostic method for in vivo diagnosis of encephalitozoonosis. Read More

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January 2018

Ultrastructural and molecular characterization of Vairimorpha austropotamobii sp. nov. (Microsporidia: Burenellidae) and Thelohania contejeani (Microsporidia: Thelohaniidae), two parasites of the white-clawed crayfish, Austropotamobius pallipes complex (Decapoda: Astacidae).

J Invertebr Pathol 2018 Jan 6;151:59-75. Epub 2017 Nov 6.

Department of Earth and Environmental Sciences, University of Pavia, Via Adolfo Ferrata 7, I-27100 Pavia, Italy.

The microsporidiosis of the endangered white-clawed crayfish Austropotamobius pallipes complex has generally been attributed to only one species, Thelohania contejeani, the agent of porcelain disease. Species identification was mostly assessed by macroscopic examination or microscopic evaluation of muscle samples rather than by molecular or ultrastructural analyses. A survey conducted on A. Read More

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January 2018
7 Reads

Production and characterization of monoclonal antibodies against Encephalitozoon intestinalis and Encephalitozoon sp. spores and their developmental stages.

Parasit Vectors 2017 Nov 9;10(1):560. Epub 2017 Nov 9.

Laboratorio de Parasitología, Universidad San Pablo CEU, Madrid, Spain.

Background: Microsporidia are intracellular obligate parasites traditionally associated with immunosuppressed patients; their detection in immunocompetent patients has increased, highlighting their possible importance as emerging pathogens. Detection of spores in stools, urine, body fluids and tissues is difficult and immunological techniques such as immunofluorescence have proved to be a useful and reliable tool in the diagnosis of human microsporidiosis. For this reason, we have produced and characterized monoclonal antibodies (MAbs) specific for Encephalitozoon intestinalis (the second most frequent microsporidian infecting humans), and other Encephalitozoon species, that can be used in different diagnostic techniques. Read More

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November 2017
6 Reads

Diabetes mellitus increases the susceptibility to encephalitozoonosis in mice.

PLoS One 2017 1;12(11):e0186954. Epub 2017 Nov 1.

Programa de Patologia Ambiental e Experimental, Universidade Paulista (UNIP), São Paulo, Brasil.

Microsporidiosis are diseases caused by opportunistic intracellular fungi in immunosuppressed individuals, as well as in transplanted patients, the elderly and children, among others. Diabetes mellitus (DM) is a metabolic disease characterized by hyperglycemia and decreased T cell response, neutrophil function, humoral immunity failure, increasing the susceptibility to infections. Here, we investigated the susceptibility of streptozotocin (STZ)-induced type I diabetic and/or immunosuppressed mice to encephalitozoonosis by Encephalitozoon cuniculi. Read More

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November 2017
3 Reads

Morphology and phylogeny of Ameson portunus n. sp. (Microsporidia) infecting the swimming crab Portunus trituberculatus from China.

Eur J Protistol 2017 Oct 27;61(Pt A):122-136. Epub 2017 Sep 27.

Key Laboratory of East China Sea Fishery Resources Exploitation, Ministry of Agriculture; East China Sea Fisheries Research Institute, China Academy of Fishery Sciences, Shanghai 200090, China. Electronic address:

Ameson portunus n. sp. is a new microsporidian species that infects the skeletal muscle of Portunus trituberculatus, a pond-reared swimming crab from China. Read More

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October 2017
2 Reads

Transmission of Pseudoloma neurophilia in Laboratory Zebrafish (Danio rerio) When Using Mass Spawning Chambers and Recommendations for Chamber Disinfection.

Zebrafish 2018 02 19;15(1):63-72. Epub 2017 Oct 19.

1 Tri-Institutional Training Program in Laboratory Animal Medicine and Science , New York, New York.

Pseudoloma neurophilia, a microsporidium that primarily infects neural tissues, is a common pathogen in laboratory zebrafish. The risk of parasite transmission with different spawning apparatuses and the effectiveness of disinfection are unknown. In this study, we spawned uninfected zebrafish with P. Read More

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February 2018

Population genetics of Enterocytozoon bieneusi in captive giant pandas of China.

Parasit Vectors 2017 Oct 18;10(1):499. Epub 2017 Oct 18.

The Key Laboratory of Animal Disease and Human Health of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Sichuan Province, China.

Background: Most studies on Enterocytozoon bieneusi are conducted based on the internal transcribed spacer (ITS) region of the rRNA gene, whereas some have examined E. bieneusi population structures. Currently, the population genetics of this pathogen in giant panda remains unknown. Read More

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October 2017

Limited effect of adaptive immune response to control encephalitozoonosis.

Parasite Immunol 2017 Dec 1;39(12). Epub 2017 Nov 1.

Institute of Parasitology, BC CAS, v.v.i., České Budějovice, Czech Republic.

This study revises our understanding of the effectiveness of cell-mediated adaptive immunity and treatment against microsporidia using molecular detection and quantification of microsporidia in immunocompetent C57Bl/6 and immunodeficient CD4 and CD8 mice for the first time. We demonstrate an intense dissemination of microsporidia into most organs within the first weeks post-infection in all strains of mice, followed by a chronic infection characterized by microsporidia persistence in CD4 and C57Bl/6 mice and a lethal outcome for CD8 mice. Albendazole application reduces microsporidia burden in C57Bl/6 and CD4 mice, whereas CD8 mice experience only a temporary effect of the treatment. Read More

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December 2017
1 Read

Infection by and genotype characteristics of Enterocytozoon bieneusi in HIV/AIDS patients from Guangxi Zhuang autonomous region, China.

BMC Infect Dis 2017 10 13;17(1):684. Epub 2017 Oct 13.

National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention; Key Laboratory of Parasite and Vector Biology, Ministry of Health, WHO Collaborating Center for Tropical Diseases, 207 Rui Jin Er Road, Shanghai, 200025, China.

Background: Enterocytozoon bieneusi has been increasingly reported to infect humans and various mammals. Microsporidia cause diarrhea in HIV-infected patients worldwide. PCR amplification and sequencing based on the internal transcribed spacer region have been used to describe the genotypes of E. Read More

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October 2017
2 Reads

First report of fatal disseminated microsporidiosis in two inland bearded dragons in Japan.

JMM Case Rep 2017 Apr 12;4(4):e005089. Epub 2017 Apr 12.

Laboratory of Veterinary Parasitology, Nippon Veterinary and Life Science University, 1-7-1 Kyonancho, Musashino, Tokyo, Japan.

is a newly described pathogen belonging to the phylum Microsporidia. In Austria and the USA, this species has been isolated from fatal and disseminated cases of captive-bred inland bearded dragons. Here, we report the case of fatal disseminated microsporidiosis caused by E. Read More

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Zoonotic microsporidia in dogs and cats in Poland.

Vet Parasitol 2017 Nov 13;246:108-111. Epub 2017 Sep 13.

Institute of Parasitology, Biology Centre of the AS CR, Branišovská 31, České Budějovice, 37005, Czech Republic.

This study investigated the prevalence, genetic diversity, and zoonotic concerns of microsporidia in household dogs and cats in Poland. A total of 126 (82 dogs and 44 cats) fecal specimens were analyzed for the presence of specific DNA of Enterocytozoon bieneusi and Encephalitozoon spp. using a nested PCR protocol amplifying the internal transcribed spacer region of the rRNA gene. Read More

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November 2017
6 Reads

Microsporidia: Obligate Intracellular Pathogens Within the Fungal Kingdom.

Microbiol Spectr 2017 04;5(2)

Department of Pathology, Division of Tropical Medicine and Parasitology.

Microsporidia are obligate intracellular pathogens related to Fungi. These organisms have a unique invasion organelle, the polar tube, which upon appropriate environmental stimulation rapidly discharges out of the spore, pierces a host cell's membrane, and serves as a conduit for sporoplasm passage into the host cell. Phylogenetic analysis suggests that microsporidia are related to the Fungi, being either a basal branch or sister group. Read More

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April 2017
5 Reads

The course of infection caused by Encephalitozoon cuniculi genotype III in immunocompetent and immunodeficient mice.

Exp Parasitol 2017 Nov 21;182:16-21. Epub 2017 Sep 21.

Institute of Parasitology, Biology Centre, Czech Academy of Sciences, České Budějovice, Czech Republic; Faculty of Agriculture, University of South Bohemia in České Budějovice, České Budějovice, Czech Republic.

Encephalitozoon cuniculi is probably the most common microsporidia which infects a wide range of vertebrates, including human. So far, four genotypes of this parasite have been identified based on the rRNA internal transcribed spacer variations. The course of infection caused by E. Read More

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November 2017
1 Read

Pragmatic Combination of Available Diagnostic Tools for Optimal Detection of Intestinal Microsporidia.

Adv Exp Med Biol 2018 ;1057:85-94

Department of Medicine, University College of Medical Sciences & Guru Teg Bahadur Hospital, Dilshad Garden, Delhi, 110095, India.

Diarrhea is a debilitating condition in HIV infected individuals and with the finding that almost 1/4 cases of diarrhea in HIV are due to microsporidia, there is a dire need to institute measures for its detection on a regular basis. Keeping this in mind the study aims to determine the burden of intestinal microsporidiosis in HIV seropositive patients presenting with and without diarrhea and to compare the ability of microscopy and PCR in its detection.The study group consisted of 120 patients divided into four groups HIV seropositive with/without diarrhea, and HIV seronegative with/without diarrhea. Read More

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January 2018
19 Reads

Microsporidial infection masquerading as graft rejection post-Descemet's stripping automated endothelial keratoplasty.

Indian J Ophthalmol 2017 Sep;65(9):869-871

Department of Cornea, Aravind Eye Hospital, Madurai, Tamil Nadu, India.

A 51-year-old immunocompetent male with a history of Fuchs' endothelial dystrophy and immature cataract who underwent Descemet's stripping automated endothelial keratoplasty with intraocular lens implantation in both eyes presented with redness and defective vision of 1-day duration in his left eye. Slit lamp examination revealed coarse superficial punctate lesions with graft edema. He was diagnosed with acute graft rejection and treated with topical steroids. Read More

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September 2017
4 Reads

Molecular diagnosis of potentially human pathogenic Enterocytozoon bieneusi and Encephalitozoon species in exotic birds in Southwestern Iran.

J Infect Public Health 2018 Mar - Apr;11(2):192-196. Epub 2017 Aug 30.

Department of Parasitology, Faculty of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.

Microsporidia are obligate intracellular parasites that produce spores. The infections caused by these parasites are mostly considered to be opportunistic in immunodeficient patients. Because of the zoonotic nature of microsporidia as well as the increasing prevalence of immunodeficiency diseases, the aim of this study was to evaluate the molecular diagnosis of Enterocytozoon bieneusi (E. Read More

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August 2017
1 Read

A DNA dot hybridization model for molecular diagnosis of parasitic keratitis.

Mol Vis 2017 24;23:614-623. Epub 2017 Aug 24.

Department of Ophthalmology, Kaohsiung Chang Gung Memorial Hospital and Chang Gung University College of Medicine, Kaohsiung, Taiwan.

Purpose: Developing a DNA dot hybridization model for diagnosing parasitic keratitis.

Methods: Newly designed oligonucleotide probes for detecting and microsporidia were tested with target reference strains of (n = 20) and microsporidia (n = 3), and non-target microorganisms, including bacteria (n = 20) and fungi (n = 20). These probes, which had passed the preliminary tests, were then assembled as a parasite dot hybridization (PDH) model for assessing 33 clinical samples from patients with clinically suspected and microsporidia keratitis, including eight positives for , 13 positives for microsporidia, and 12 negatives for both pathogens. Read More

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April 2018
6 Reads

Molecular characterization of Enterocytozoon bieneusi isolates in laboratory macaques in north China: zoonotic concerns.

Parasitol Res 2017 Oct 31;116(10):2877-2882. Epub 2017 Aug 31.

Heilongjiang Key Laboratory for Zoonosis, College of Veterinary Medicine, Northeast Agricultural University, 600 Changjiang Road, Harbin, Heilongjiang, 150030, China.

The significance of wild and zoo nonhuman primates (NHPs) as potential sources of human Enterocytozoon bieneusi infections has been increasingly appreciated, while the role of laboratory NHPs in zoonotic transmission of microsporidiosis remains elusive. In this study, the infection rate, genetic characteristic, and zoonotic potential of E. bieneusi were investigated for 205 laboratory macaques in Beijing, north China. Read More

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October 2017
8 Reads

Evidence-Based Advances in Rabbit Medicine.

Vet Clin North Am Exot Anim Pract 2017 Sep;20(3):749-771

Department of Veterinary Clinical Sciences, Center for Veterinary Health Sciences, Oklahoma State University, 2065 West Farm Road, Stillwater, OK 74078, USA. Electronic address:

Rabbit medicine has been continuously evolving over time with increasing popularity and demand. Tremendous advances have been made in rabbit medicine over the past 5 years, including the use of imaging tools for otitis and dental disease management, the development of laboratory testing for encephalitozoonosis, or determination of prognosis in rabbits. Recent pharmacokinetic studies have been published, providing additional information on commonly used antibiotics and motility-enhancer drugs, as well as benzimidazole toxicosis. Read More

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September 2017
6 Reads

Effects of selected Indonesian plant extracts on E. cuniculi infection in vivo.

Exp Parasitol 2017 Oct 2;181:94-101. Epub 2017 Aug 2.

Department of Parasitology, Faculty of Veterinary Medicine, Gadjah Mada University, Yogyakarta, Indonesia; Department of Botany and Zoology, Faculty of Science, Masaryk University, Brno, Czech Republic.

The present study was conducted to evaluate the methanolic extracts from several plant leaves widely used in traditional medicine to cure digestive tract disorders and in the self-medication of wild animals such as non-human primates, namely Archidendron fagifolium, Diospyros sumatrana, Shorea sumatrana, and Piper betle leaves, with regard to their antimicrosporidial activity against Encephalitozoon cuniculi in immunocompetent BALB/c mice determined using molecular detection of microsporidial DNA (qPCR) in various tissues and body fluids of infected, treated mice. Of the plant extracts tested, Diospyros sumatrana provided the most promising results, reducing spore shedding by 88% compared to untreated controls. Moreover, total burden per 1 g of tissue in the D. Read More

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October 2017
1 Read

A case study of Desmozoon lepeophtherii infection in farmed Atlantic salmon associated with gill disease, peritonitis, intestinal infection, stunted growth, and increased mortality.

Parasit Vectors 2017 Aug 2;10(1):370. Epub 2017 Aug 2.

Norwegian Veterinary Institute, P.O.Box 750 Dep., N-0106, Oslo, Norway.

Background: In September 2008, a disease outbreak characterized by acute, severe gill pathology and peritonitis, involving the gastrointestinal tract, was observed in an Atlantic salmon (Salmo salar L.) farm in north-western Norway. During subsequent sampling in November 2008 and January 2009, chronic proliferative gill inflammation and peritonitis was observed. Read More

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August 2017
1 Read

Functional characterization of an aquaporin from a microsporidium, Nosema bombycis.

PLoS One 2017 27;12(7):e0181703. Epub 2017 Jul 27.

Jiangsu University of Science and Technology, Zhenjiang, Jiangsu Province, China.

Microsporidia are a diverse group of eukaryotic organisms, capable of causing parasitic infections in both vertebrates and invertebrates. During the germination process, there is an increase in the osmotic pressure of microsporidian spores. As part of this study, we cloned a homologous aquaporin gene in Nosema bombycis, and named it Nosema bombycis aquaporin (NbAQP). Read More

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September 2017
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Application of mass spectrometry to elucidate the pathophysiology of Encephalitozoon cuniculi infection in rabbits.

PLoS One 2017 19;12(7):e0177961. Epub 2017 Jul 19.

University of Miami - Miller School of Medicine, Division of Comparative Pathology, Department of Pathology & Laboratory Medicine, Miami, Florida, United States of America.

Encephalitozoon cuniculi is a microsporidian species which can induce subclinical to serious disease in mammals including rabbits, a definitive natural host. The pathophysiology of infection has not been comprehensively elucidated. In this exploratory study, we utilized two mass spectrometry approaches: first, the analysis of the humoral response by profiling the microsporidian antigens as revealed by Western blot screening, and second, implementing the iTRAQ®-labeling protocol to focus on the changes within the host proteome during infection. Read More

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September 2017
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Phylogeny and morphology of Ovipleistophora diplostomuri n. sp. (Microsporidia) with a unique dual-host tropism for bluegill sunfish and the digenean parasite Posthodiplostomum minimum (Strigeatida).

Authors:
J Lovy S E Friend

Parasitology 2017 Dec 12;144(14):1898-1911. Epub 2017 Jul 12.

Office of Fish and Wildlife Health and Forensics,N.J. Division of Fish and Wildlife,605 Pequest Road,Oxford, NJ 07863,USA.

Microsporidia are diverse opportunistic parasites abundant in aquatic organisms with some species hyperparasitic in digenean parasites. In the current study, we describe a unique microsporidian parasite, Ovipleistophora diplostomuri n. sp. Read More

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December 2017
26 Reads

Detection of in White Yaks in Gansu Province, China.

Biomed Res Int 2017 6;2017:5790181. Epub 2017 Jun 6.

State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, Gansu Province 730046, China.

, the most common zoonotic pathogen of microsporidiosis, has been found in various animals and humans, but no information is available concerning the prevalence and genotypes of in white yaks . In the present study, 353 faecal samples from white yaks in Tianzhu Tibetan Autonomous County, Gansu Province, Northwestern China, were collected and examined by PCR amplification of the internal transcribed spacer gene to estimate prevalence and identify their genotypes. Of the 353 faecal samples, 4 (1. Read More

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March 2018
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