4,377 results match your criteria Microscopy research and technique[Journal]


Light and transmission electron microscopic structure of skin glands and dermal scales of a caecilian amphibian Gegeneophis ramaswamii, with a note on antimicrobial property of skin gland secretion.

Microsc Res Tech 2019 Apr 19. Epub 2019 Apr 19.

Department of Animal Science, Central University of Kerala, Kasaragod, Kerala, India.

Amphibian skin secretions contain a variety of bioactive compounds that are involved in diverse roles such as communication, homeostasis, defence against predators, pathogens, and so on. Especially, the caecilian amphibians possess numerous cutaneous glands that produce the secretory material, which facilitate survival in their harsh subterranean environment. Inspite of the fact that India has a fairly abundant distribution of caecilian amphibians, there has hardly been any study on their skin and its secretion. Read More

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http://dx.doi.org/10.1002/jemt.23276DOI Listing

Comparative light and scanning electron microscopy in authentication of adulterated traded medicinal plants.

Microsc Res Tech 2019 Apr 17. Epub 2019 Apr 17.

Department of Plant Sciences, Quaid-i-Azam University, Islamabad, Pakistan.

The medicinal plants are utilized globally considering the cheap and chemical free source, but their correct identification and authentication is prerequisite for safety and efficacy of plant-based medicines. The present study encompassed traded medicinal plants (16) with high therapeutic value from diverse families like Brassicaceae, Berberidaceae, Malvaceae, Salicaceae, Myrtaceae, Papilionaceae, Ascelpiadaceae, Colchicaceae, Violaceae, and Vitaceae for detailed microscopic study of characters that is, morphology, pollen shape and sizes, P/E ratio, pore length and width, spine length, colpi dimensions, and exine sculpture pattern. The plants showed noteworthy differences in microscopy of Wattakaka volubilis having pollinia, translator and corpusculum like structures while pores were visible in Colchicum luteum, Alcea rosea, and Hibiscus syriacus. Read More

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http://dx.doi.org/10.1002/jemt.23266DOI Listing

Disinfection and surface changes of gutta-percha cones after immersion in sodium hypochlorite solution containing surfactant.

Microsc Res Tech 2019 Apr 16. Epub 2019 Apr 16.

Department of Dentistry/Endodontics, Federal University of Santa Catarina-UFSC, Florianópolis, Santa Catarina, Brazil.

In this study, we evaluated the effectiveness of 1 and 5.25% NaOCl solutions, with or without surfactant, in disinfecting gutta-percha cones. Surface changes of the gutta-percha cones after disinfection were also assessed under scanning electron microscopy (SEM). Read More

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http://dx.doi.org/10.1002/jemt.23279DOI Listing

Histological description of immature Chironomus columbiensis (Diptera: Chironomidae): A potential contribution to environmental monitoring.

Microsc Res Tech 2019 Apr 11. Epub 2019 Apr 11.

Grupo de Investigación GEBIOME, Departamento de Ciencias Biológicas, Facultad de Ciencias Exactas y Naturales, Universidad de Caldas, Caldas, Colombia.

Chironomids have been widely used in environmental monitoring and toxicity assays. Assessment criteria for chironomids range from the molecular-biochemical level to the population level. However, the use of markers to evaluate cellular and anatomical changes in organs and systems of individuals under contaminant exposure is still incipient. Read More

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https://onlinelibrary.wiley.com/doi/abs/10.1002/jemt.23278
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http://dx.doi.org/10.1002/jemt.23278DOI Listing
April 2019
4 Reads

Lungs nodule detection framework from computed tomography images using support vector machine.

Microsc Res Tech 2019 Apr 11. Epub 2019 Apr 11.

Department of EE, COMSATS University Islamabad, Wah Campus, Islamabad, Pakistan.

The emergence of cloud infrastructure has the potential to provide significant benefits in a variety of areas in the medical imaging field. The driving force behind the extensive use of cloud infrastructure for medical image processing is the exponential increase in the size of computed tomography (CT) and magnetic resonance imaging (MRI) data. The size of a single CT/MRI image has increased manifold since the inception of these imagery techniques. Read More

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https://onlinelibrary.wiley.com/doi/abs/10.1002/jemt.23275
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http://dx.doi.org/10.1002/jemt.23275DOI Listing
April 2019
2 Reads
1.170 Impact Factor

Identification of atrial fibrillation-associated lncRNAs in atria from patients with rheumatic mitral valve disease.

Microsc Res Tech 2019 Apr 11. Epub 2019 Apr 11.

Department of Cardiovascular Surgery, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, China.

Objective: We analysed lncRNA expression profiles in atrial samples from patients with rheumatic mitral valve disease (RMVD) to identify the potential differences in atrial fibrillation (AF)-associated lncRNAs between RMVD patients with AF and sinus rhythm (SR).

Methods: Masson's trichrome staining and scanning electron microscopy were performed to evaluate the tissue morphology. Western blotting was performed to detect the expression of fibrosis-related proteins. Read More

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http://dx.doi.org/10.1002/jemt.23261DOI Listing
April 2019
2 Reads

The effect varied scanning electron microscopy desiccation techniques has on demineralized dentin.

Microsc Res Tech 2019 Apr 11. Epub 2019 Apr 11.

Department of Biology, Maryville College, Maryville, Tennessee.

The study objective was to assess (a) the effect of a rubbing-application of ethylenediaminetetraacetic acid (EDTA) or citric acid (CA) has on the ultrastructure of surface dentin and (b) the effect of two scanning electron microscopy (SEM) desiccation preparation techniques have on the collagen surface produced. Treatment regions on proximal root surfaces of extracted human teeth were root planned to expose dentin. Cotton pellets soaked in either 30% CA or 24% EDTA solution were rubbed on the treatment region then processed for SEM using one of two desiccation techniques, that is, (a) critically point dried from liquid CO (control) or (b) air-dried from tetramethylsilane (experimental). Read More

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http://dx.doi.org/10.1002/jemt.23274DOI Listing
April 2019
1 Read

Scanning electron microscopy leads to identification of novel nonedible oil seeds as energy crops.

Microsc Res Tech 2019 Apr 5. Epub 2019 Apr 5.

Biodiesel Lab, Department of Plant Sciences, Quaid-i-Azam University, Islamabad, Pakistan.

Currently, exploration of alternative energy resources is hotly debated among the scientific community owing to rising energy crises and environmental issues. Biodiesel, as renewable energy source proves to be a better option and substitute to petro diesel. In this regard, nonedible seeds could be a better feedstock for synthesizing biodiesel due to their cost effectiveness and environmental friendly attributes. Read More

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http://dx.doi.org/10.1002/jemt.23265DOI Listing
April 2019
1 Read

Effect of long-term exposure of silver nanoparticles on growth indices, hematological and biochemical parameters and gonad histology of male goldfish (Carassius auratus gibelio).

Microsc Res Tech 2019 Apr 4. Epub 2019 Apr 4.

Department of Chemical, Biological, Pharmaceutical and Environmental Sciences, University of Messina, Messina, Italy.

Studying the impact of emerging pollutants such as nanoparticles is necessary to reveal the adverse effect. In this study, the effects of silver nanoparticles (Ag-NPs) on hematological, biochemical, and gonad histopathological indices of male goldfish were examined. Sublethal toxicity were calculated based on acute toxicity and three dosages were selected. Read More

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http://dx.doi.org/10.1002/jemt.23271DOI Listing
April 2019
2 Reads

Microstructural morphology of dermal and oral denticles of the sharpnose sevengill shark Heptranchias perlo (Elasmobranchii: Hexanchidae), a deep-water species.

Microsc Res Tech 2019 Apr 4. Epub 2019 Apr 4.

Central de Facilidades à Pesquisa, Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, São Paulo, SP, Brazil.

The dermal denticles are among the unique morphological adaptations of sharks, which have been acquired throughout their long evolutionary process of more than 400 million years. Species-specific morphological characteristics of these structures has been applied specially as tools for functional and taxonomic (family-level) studies. Nevertheless, few studies have explored the diversity of denticle structure in different around the body and oral cavity. Read More

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http://dx.doi.org/10.1002/jemt.23273DOI Listing
April 2019
1 Read

Uncommon hepatic macrophagic foamy-cell nodules in Iberian barbel (Luciobarbus bocagei Steindachner, 1864) from the Vizela River (Portugal).

Microsc Res Tech 2019 Apr 4. Epub 2019 Apr 4.

Laboratory of Histology and Embryology, Department of Microscopy, ICBAS - Institute of Biomedical Sciences Abel Salazar, UPorto - University of Porto, Porto, Portugal.

The main intent of this work (after the by chance finding, in archived histological slides) is to characterize one previously non-described liver lesion of the Iberian barbel from the Vizela River (Portugal). This ran through a textile and dyeing industrial region. The lesion type was made of groups of foamy cells (presumptive macrophages), which appear either as a "smaller non-nodular form," without a connective tissue capsule and displaying an irregular profile, or as a "bigger nodular form," presenting a thin capsule and a circular profile. Read More

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http://dx.doi.org/10.1002/jemt.23260DOI Listing
April 2019
3 Reads

Characterization of spatial patterns of dental restorative nanocomposites.

Microsc Res Tech 2019 Apr 1. Epub 2019 Apr 1.

School of Dentistry, Faculty of Medicine, Department of Restorative Dentistry and Endodontics, Clinic of Dentistry of Vojvodina, University of Novi Sad, Novi Sad, Serbia.

The aim of this study was to provide important insights into the effects of four different dental polishing protocols (one single-step and one multi-step either followed or not by diamond paste polishing) on the 3D surface morphology of two representative dental resin-based nanocomposites (a nanofilled and a nanohybrid composite) by means of digital image analysis and processing techniques. The 3D surface morphology was investigated by atomic force microscopy. Segmentation, statistics of height distributions (described by statistical parameters, according to ISO 25178-2: 2012) and Minkowski functionals were applied to the images to characterize the spatial patterns of analyzed samples at micrometer scale. Read More

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https://onlinelibrary.wiley.com/doi/abs/10.1002/jemt.23270
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http://dx.doi.org/10.1002/jemt.23270DOI Listing
April 2019
2 Reads

Plasmodium species aware based quantification of malaria parasitemia in light microscopy thin blood smear.

Microsc Res Tech 2019 Apr 1. Epub 2019 Apr 1.

Department of Computer Science, Islamia College Peshawar, KPK, Pakistan.

Malaria is a serious worldwide disease, caused by a bite of a female Anopheles mosquito. The parasite transferred into complex life round in which it is grown and reproduces into the human body. The detection and recognition of Plasmodium species are possible and efficient through a process called staining (Giemsa). Read More

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http://dx.doi.org/10.1002/jemt.23269DOI Listing
April 2019
1 Read

Morpho-palynological investigations of natural resources: A case study of Surghar mountain district Mianwali Punjab, Pakistan.

Microsc Res Tech 2019 Apr 1. Epub 2019 Apr 1.

Department of Plant Sciences, Quaid-i-Azam University, Islamabad, Pakistan.

Surghar mountain belt has comparatively less natural resources of floral diversity because it is composed of minerals of different kinds making it less favorable for the growth of different vegetation. The pollen morphology of some selected plants from Surghar belt Mainwali has been evaluated. The pollen grains were measured and demonstrated using scanning electron microscopy (SEM). Read More

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http://dx.doi.org/10.1002/jemt.23252DOI Listing
April 2019
2 Reads

Utilization of foliar cuticle morphology for the identification of weedy grasses.

Microsc Res Tech 2019 Apr 1. Epub 2019 Apr 1.

Department of Plant Sciences, Quaid-i-Azam University, Islamabad, Pakistan.

For the robust identification of weeds of taxonomically complex families like Poaceae, scanning electron microscopy (SEM) and light microscopy (LM) are of significant implications. In this study, SEM and LM methods were used by investigating 12 Pakistani weedy grass species as an example for foliar epidermal micromorphological characters for its role in the identification of grasses species. Qualitative and quantitative foliar micromorphological variations in stomatal type, number, guard cells, subsidiary cells, silica bodies and trichomes were studied. Read More

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http://dx.doi.org/10.1002/jemt.23272DOI Listing
April 2019
1 Read
1.170 Impact Factor

Histological analysis of biocompatibility of different surgical adhesives in subcutaneous tissue.

Microsc Res Tech 2019 Mar 29. Epub 2019 Mar 29.

Department of Pathology, School of Veterinary Medicine, Federal University of Campina Grande, Patos, Paraíba, Brazil.

The focus of this study was to test the hypothesis that there would be no difference between the biocompatibility of cyanoacrylate-based adhesives in rat subcutaneous tissues. In total, 60 male Wistar rats were used, and divided into four groups (n = 15): Group C (control, PVA-polyvinyl alcohol sponge), Group NO (N-butyl-2-octylcyanoacrylate), Group NH (n-hexyl-cyanoacrylate), and Group EC (Ethyl-cyanoacrylate). The animals were sacrificed after time intervals of 7, 15, and 30 days and tissues were analyzed under optical microscope as regards the events of inflammatory infiltrate, edema, necrosis, granulation tissue, giant cells, young fibroblasts, and collagen formation. Read More

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http://dx.doi.org/10.1002/jemt.23267DOI Listing

The effectiveness of glass ionomer cement as a fiber post cementation system in endodontically treated teeth.

Microsc Res Tech 2019 Mar 28. Epub 2019 Mar 28.

Department of Restorative Dentistry, Araraquara School of Dentistry, University of Sao Paulo State, Araraquara, Brazil.

This study compared the performance of a glass ionomer (GC Gold Label 1, GIC) as a fiber post cementation system for glass fiber posts with a self-adhesive resin cement (Relyx U200, RUC) and a conventional resin cement system (Scotchbond Muli-Purpose and Relyx ARC, RAC). Thirty endodontically treated canines were randomly divided in three groups (n = 10), according to the fiber post cementation system: (RAC)-Scotchbond Multi-Purpose and Relyx X ARC; (RUC)-Relyx U200 and (GIC)-GC Gold Label 1 Luting & Lining. Rhodamine was incorporated into the cementation system prior to the fiber post cementation. Read More

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http://dx.doi.org/10.1002/jemt.23268DOI Listing
March 2019
1 Read

Microstructure and material properties of hind wings of a bamboo weevil Cyrtotrachelus buqueti (Coleoptera: Curculionidae).

Authors:
Xin Li Ce Guo

Microsc Res Tech 2019 Mar 28. Epub 2019 Mar 28.

Institute of Bio-inspired Structure and Surface Engineering, Nanjing University of Aeronautics and Astronautics, Nanjing, China.

Wings of flying insects, as representative biomaterials, are composed of a flexible membrane and a stiff vein structure that are prone to bending and deformation under aerodynamic forces. Therefore, we must investigate the application value of insect wings in the field of engineering design from the perspective of bionics, which is a new challenge. In this study, we measured the mechanical properties of the hind wings of Cyrtotrachelus buqueti including "dried" and "fresh" samples. Read More

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http://dx.doi.org/10.1002/jemt.23258DOI Listing
March 2019
1 Read

Atomic force microscopy and energy dispersive X-ray spectrophotometry analysis of reciprocating and continuous rotary nickel-titanium instruments following root canal retreatment.

Microsc Res Tech 2019 Mar 27. Epub 2019 Mar 27.

Department of Endodontics, Faculty of Dentistry, Kütahya Sağlık Bilimleri University, Kütahya, Turkey.

The aim was to examine the effect of retreatment process on the surface roughness and nickel titanium (NiTi) composition of ProTaper Universal Retreatment (PTUR; consists of 3 files; D1, D2, D3) and WaveOne Gold (WOG) (primary) instruments. Twenty extracted mandibular molar teeth with severe curved (30-40°) mesial roots were selected and divided into two groups (n = 10) based on the instrument used for the removal of the root canal filling. Before and after using the instruments in two canals, they were subjected to atomic force microscopy (AFM) and energy dispersive X-ray spectrophotometry (EDX) analysis. Read More

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http://dx.doi.org/10.1002/jemt.23264DOI Listing
March 2019
1 Read

A protocol for processing the delicate larval and prepupal salivary glands of Drosophila for scanning electron microscopy.

Microsc Res Tech 2019 Mar 26. Epub 2019 Mar 26.

Laboratory of Developmental Genetics, Institute of Experimental Endocrinology, Biomedical Research Center, Slovak Academy of Sciences, Bratislava, Slovakia.

Although scanning electron microscopy (SEM) has been broadly used for the examination of fixed whole insects or their hard exoskeleton-derived structures, including model organisms such as Drosophila, the routine use of SEM to evaluate vulnerable soft internal organs and tissues was often hampered by their fragile nature and frequent surface contamination. Here, we describe a simple four-step protocol that allows for the reliable and reproducible preparation of the larval and prepupal salivary glands (SGs) of Drosophila for SEM devoid of any surface contamination. The steps are to: first, proteolytically digest the adhering fat body; second, use detergent washes to remove contaminating coarse tissue fragments, including sticky remnants of the fat body; third, use nonionic emulsifying polysorbate emulsifiers to remove fine contaminants from the SGs surface; and fourth, use aminopolycarboxylate-based chelating agents to detach sessile hemocytes. Read More

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http://dx.doi.org/10.1002/jemt.23263DOI Listing

Chemical and morphological evaluation of enamel and dentin near cavities restored with conventional and zirconia modified glass ionomer subjected to erosion-abrasion.

Microsc Res Tech 2019 Mar 24. Epub 2019 Mar 24.

Laboratório de Cerâmicas Avançadas, Instituto de Pesquisa e Desenvolvimento (IP&D), Universidade do Vale do Paraíba (UNIVAP), São Paulo, Brazil.

Microenergy dispersive X-ray fluorescence (μ-EDXRF) spectroscopy and scanning electron microscopy (SEM) were used to test the hypothesis that zirconia modified glass ionomer cement (GIC) could improve resistance to erosion-abrasion to a greater extent than conventional cement. Bovine enamel (n = 40) and dentin (n = 40) samples were prepared with cavities, filled with one of the two restorative materials (GIC: glass-ionomer cement or ZrGIC: zirconia-modified GIC). Furthermore, the samples were treated with abrasion-saliva (AS) or abrasion-erosion cycles (AE). Read More

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http://dx.doi.org/10.1002/jemt.23259DOI Listing

Morphology of the mandibular gland of the ant Paraponera clavata (Hymenoptera: Paraponerinae).

Microsc Res Tech 2019 Mar 22. Epub 2019 Mar 22.

Programa de Pós-Graduação em Biodiversidade, Ambiente e Saúde, Departamento de Biologia e Química, Universidade Estadual do Maranhão, Caxias, Maranhão, Brazil.

The ant Paraponera clavata (Fabricius, 1775) is the only extant species of Paraponerinae and is widely distributed in Brazilian forests. Aspects of its biology are documented extensively in the literature; however, knowledge of P. clavata internal morphology, specifically of exocrine glands, is restricted to the venom apparatus. Read More

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http://dx.doi.org/10.1002/jemt.23242DOI Listing

Macromorphological and micromorphological seed features of selected taxa of Caesalpiniaceae using light and scanning electron microscopy.

Microsc Res Tech 2019 Mar 22. Epub 2019 Mar 22.

Department of Plant Sciences, Quaid-i-Azam University, Islamabad, Pakistan.

Seed micromorphological and macromorphological characteristics of 12 taxa of Caesalpiniaceae from Pakistan have been studied, using light and scanning electron microscopy, to investigate the importance of seed coat features as a taxonomic tool. Great variations have been observed in color, shape, dimension, and seed surface pattern among the different genera of the family. A taxonomic key was prepared for the studied taxa. Read More

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http://dx.doi.org/10.1002/jemt.23255DOI Listing
March 2019
6 Reads

Silver release of Ag (I) doped hydroxyapatite: In vitro study.

Microsc Res Tech 2019 Mar 22. Epub 2019 Mar 22.

Faculty of Engineering, Department of Electric and Electronics Engineering, Karabuk University, Karabuk, Turkey.

A material is produced by doping of silver (Ag (I)) which has antibacterial property to nano hydroxyapatite (nHAp), to remove the hipersensitivity in the teeth by closing the dentine tubules or dental micro cracks of the teeth and effective against for some bacteria. The doping of Ag (I) can also produces a toxic effect. Ag (I) can be released from the structure as a result of biological, physical and chemical effects and may cause toxicity. Read More

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https://onlinelibrary.wiley.com/doi/abs/10.1002/jemt.23243
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http://dx.doi.org/10.1002/jemt.23243DOI Listing
March 2019
4 Reads

Microscopic and phytochemical techniques as a tool for authentication of herbal drug chiraita: Swertia cordata (G. Don) C.B. Clarke.

Microsc Res Tech 2019 Mar 21. Epub 2019 Mar 21.

Department of Botany, Hazara University, Mansehra, Pakistan.

Swertia cordata (G. Don) C.B. Read More

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http://dx.doi.org/10.1002/jemt.23257DOI Listing
March 2019
3 Reads

Morphology and mechanical properties of the dorsal bony plates in the Chinese sturgeon (Acipenser sinensis).

Microsc Res Tech 2019 Mar 20. Epub 2019 Mar 20.

College of Mechanical and Electrical Engineering, Nanjing University of Aeronautics and Astronautics, Nanjing, 210016, China.

The morphology, composition, and mechanical properties of the dorsal bony plates in the Chinese sturgeon (Acipenser sinensis) were determined in this study. Scanning electron microscopy images of the bony plates revealed a ridge-like shape similar to that of traditional Chinese architecture and displayed hierarchical microstructures whose the degree of mineralization decreased from the surface layer to the internal layer. Moreover, the crests in the central part of the bony plates displayed porous characteristics. Read More

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http://dx.doi.org/10.1002/jemt.23256DOI Listing

Efficacy of reciprocating systems for removing root filling material plus complementary cleaning methods in flattened canals: Microtomography and scanning electron microscopy study.

Microsc Res Tech 2019 Mar 19. Epub 2019 Mar 19.

Department of Restorative Dentistry, Dental Materials and Endodontics, Bauru Dental School, University of São Paulo, São Paulo, Brazil.

The aim of this study was to evaluate three reciprocating systems and complementary cleaning methods on filling material removal during retreatment of flattened canals. Thirty-six mandibular incisors were prepared using rotary instruments up to size 25.08 and filled using the single-cone technique. Read More

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http://dx.doi.org/10.1002/jemt.23253DOI Listing

Analysis of efficacy of the self-assembling peptide-based remineralization agent on artificial enamel lesions.

Microsc Res Tech 2019 Mar 18. Epub 2019 Mar 18.

Department of Pediatric Dentistry, Istanbul University Faculty of Dentistry, Istanbul, Turkey.

The objective of this in vitro study was to analyze and compare the biomimetic remineralizing efficacy of the self-assembling peptide (P11-4) with agents containing casein phoshopeptide-amorhous calcium phosphate fluoride (CPP-ACFP) and sodium fluoride (NaF) on artificial caries lesions using DIAGNOdent and micro-computed tomography (μCT). Artificial enamel lesions were prepared on extracted impacted sound mandibular third molars. The samples were randomly allocated to four groups (n = 8): Group 1, P11-4 (Curodont Repair, Credentis AG, Switzerland); Group 2, CPP-ACFP (MI Varnish, GCCo. Read More

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http://dx.doi.org/10.1002/jemt.23254DOI Listing

The effects of osmium tetroxide post-fixation and drying steps on leafy liverwort ultrastructure study by scanning electron microscopy.

Microsc Res Tech 2019 Mar 13. Epub 2019 Mar 13.

Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Indonesia, Depok, Indonesia.

Leafy liverwort is one of the most abundant and diverse plants in Indonesia. Their high variation and beneficial secondary metabolites contained in the oil bodies have attracted researchers' attention. The ultrastructural analysis of leafy liverworts is important as a means of species identification and also for further exploration of their oil bodies. Read More

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http://dx.doi.org/10.1002/jemt.23251DOI Listing
March 2019
1 Read

Comparison of microtensile bond strength and resin-dentin interfaces of two self-adhesive flowable composite resins by using different universal adhesives: Scanning electron microscope study.

Microsc Res Tech 2019 Mar 13. Epub 2019 Mar 13.

Department of Pediatric Dentistry, Faculty of Dentistry, Cumhuriyet University, Sivas, Turkey.

The aim of this in-vitro study was to evaluate microtensile bond strength (μTBS) of two different self-adhesive composites (SACs) on the permanent dentin by applying five different universal adhesive systems. In this study, two different SACs [Vertise Flow (VF), Fusio Liquid Dentin (FLD)] and five different bonding systems [Clearfil Universal Bond Quick (CUB), Single Bond Universal (SBU), All Bond Universal (ABU), Prime Bond Universal (PBU), Futurabond U (FBU)] were used. A total of 22 groups were created in which SACs were applied without adhesive and with five different universal bonding agents in total-etch (TE) and self-etch (SE) modes. Read More

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http://dx.doi.org/10.1002/jemt.23250DOI Listing

Taxonomic study of subfamily Nepetoideae (Lamiaceae) by polynomorphological approach.

Microsc Res Tech 2019 Mar 12. Epub 2019 Mar 12.

Department of Botany, Government College University, Lahore, Pakistan.

The present study is insight into pollen morphology for characterizing species and their utility in the taxonomic separation of certain taxa of subfamily Nepetoideae (Lamiaceae) from Pakistan. The pollen micromorphology of 11 species of the Nepetoideae was analyzed and documented using light microscopy and scanning electron microscopy (SEM) for both qualitative and quantitative characteristics. Most species have hexazonocolpate pollen grains but trizonocolpate and tetrazonocolpate pollen with circular and oval amb were also rarely observed in Mentha spicata. Read More

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http://doi.wiley.com/10.1002/jemt.23249
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http://dx.doi.org/10.1002/jemt.23249DOI Listing
March 2019
7 Reads
1.170 Impact Factor

Investigating Schizocarp morphology as a taxonomic tool in study of Apiaceae family by utilizing LM and SEM techniques.

Microsc Res Tech 2019 Mar 9. Epub 2019 Mar 9.

Department of Environmental Sciences, Fatima Jinnah Women University, Rawalpindi, Pakistan.

In present study, the schizocarp morphology of 14 species belonging to Apiaceae family has been investigated. Light microscopy (LM) and scanning electron microscopy (SEM) have been utilized to highlight qualitative and quantitative features of studied species. Variations have been observed in macro- and micro-morphological features such as color, shape, symmetry, length, width, apex, epicuticular projections, surface patterns, anticlinal, and periclinal wall patterns. Read More

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http://dx.doi.org/10.1002/jemt.23248DOI Listing
March 2019
1 Read

Neural network sliding mode controller of atomic force microscope-based manipulation with different cantilever probes.

Microsc Res Tech 2019 Mar 6. Epub 2019 Mar 6.

Faculty of Electrical, Biomedical and Mechatronics Engineering, Qazvin Branch, Islamic Azad University, Qazvin, Iran.

Development of nanotechnology has given rise to various applications, including the nano-manipulation process within small-size environments. The implementation of such processes requires the use of tools and proper equipment and understanding of various factors influencing it. One such tool is the atomic force microscope (AFM) and its probe, used for imaging surfaces and manipulation tools. Read More

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http://dx.doi.org/10.1002/jemt.23246DOI Listing
March 2019
1 Read

Bone demineralization promotes superior spread of preosteoblast in culture.

Microsc Res Tech 2019 Mar 6. Epub 2019 Mar 6.

Department of Prosthodontics and Periodontics, Bauru School of Dentistry, University of São Paulo, Bauru, São Paulo, Brazil.

Previous studies have shown substances capable of similar effects of demineralization, accelerating the process of bone remodeling. This study investigated preosteoblasts behavior in cell culture after bone demineralization with citric acid and tetracycline. Seventy-four Wistar rats provided 144 calvarial bone samples, 126 of which were randomly divided in seven groups according to the treatment given to the surface: no demineralization (C), citric acid (CA), tetracycline (TCN) during 15, 30, and 60 s. Read More

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http://dx.doi.org/10.1002/jemt.23247DOI Listing
March 2019
6 Reads

Dentinal tubule penetration of endodontic sealers after nonthermal plasma treatment: A confocal laser scanning microscopy study.

Microsc Res Tech 2019 Feb 27. Epub 2019 Feb 27.

Faculty of Agriculture, Department of Animal Science Biometry and Genetic, Eskisehir Osmangazi University, Eskişehir, Turkey.

One of the factors affecting the success of endodontic treatment is to fill the root canal system hermetically. The aim of this in vitro study was to evaluate the effect of nonthermal plasma (NP) on dentinal tubule penetration of root canal sealers using confocal laser scanning microscopy. Forty mandibular premolar teeth were selected and the root canals were prepared with large-Waveone-Gold rotary-files. Read More

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http://dx.doi.org/10.1002/jemt.23237DOI Listing
February 2019
1 Read

Functionalized magnetic nanoparticles attenuate cancer cells proliferation: Transmission electron microscopy analysis.

Microsc Res Tech 2019 Feb 26. Epub 2019 Feb 26.

Department of Stem Cell Biology, Institute for Research and Medical Consultations (IRMC), Imam Abdulrahman Bin Faisal University, Dammam, Saudi Arabia.

The penetration and transportation of nanoparticles (NPs) inside the cancer cells is critical to study. In this article, cancer cells (HCT-116) were treated with functionalized magnetic NPs for the period of 48 hr and studied their ultrastructure by transmission electron microscopy (TEM). The NPs-treated cells were prepared by chemical fixation and sliced into electron-transparent arbitrary sections (200 × 200 μm ) by ultramicrotome. Read More

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http://dx.doi.org/10.1002/jemt.23245DOI Listing
February 2019
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Implication of palynological techniques for the authentication of adulterated drugs traded with the same name in different herbal markets of district Lahore, Pakistan.

Microsc Res Tech 2019 Feb 25. Epub 2019 Feb 25.

Department of Botany, Lahore College For Women University, Lahore, Pakistan.

Adulteration in traded medicinal plants is a significant issue nowadays and use of these adulterated medicinal plants can impose harmful impact to end user. However, this problem can be overcome by ensuring the identification of traded medicinal plants which are used in making different herbal medicines. In this regard, palynological markers are considered to be an important taxonomic tool in the identification of original medicinal plant from its adulterant. Read More

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http://dx.doi.org/10.1002/jemt.23240DOI Listing
February 2019
2 Reads
1.170 Impact Factor

Foliar and stem epidermal anatomy of the tribe Cynoglosseae (Boraginaceae) and their taxonomic significance.

Microsc Res Tech 2019 Feb 25. Epub 2019 Feb 25.

State Key Laboratory of Systematic and Evolutionary Botany, Institute of Botany, Chinese Academy of Sciences, Beijing, China.

Foliar and stem epidermal anatomical features of the tribe Cynoglosseae have been studied in detail for the taxonomic identification using light microscopy (LM) and scanning electron microscopic (SEM) techniques. A comparative study was conducted on different plant parts (leaf and stem epidermal anatomy) of 25 species belonging to eight genera of the tribe Cynoglosseae (Boraginaceae) collected from different phytogeographical regions of Iran for the first time. Different qualitative and quantitative characteristics were observed in detail using LM and SEM. Read More

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http://dx.doi.org/10.1002/jemt.23223DOI Listing
February 2019
1 Read
1.170 Impact Factor

Brain tumor detection and classification: A framework of marker-based watershed algorithm and multilevel priority features selection.

Microsc Res Tech 2019 Feb 23. Epub 2019 Feb 23.

Department of EE, COMSATS University Islamabad, Wah Cantt, Pakistan.

Brain tumor identification using magnetic resonance images (MRI) is an important research domain in the field of medical imaging. Use of computerized techniques helps the doctors for the diagnosis and treatment against brain cancer. In this article, an automated system is developed for tumor extraction and classification from MRI. Read More

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http://dx.doi.org/10.1002/jemt.23238DOI Listing
February 2019
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Morphology and distribution of antennal sensilla in egg parasitoid, Ooencyrtus nezarae (Hymenoptera: Encyrtidae).

Microsc Res Tech 2019 Feb 25. Epub 2019 Feb 25.

Institute of Life Science (BK21+ Program)/Institute of Agriculture and Life Science, Gyeongsang National University, Jinju 52828, Republic of Korea.

Morphology of antennal sensilla and their distribution were investigated in adults of Ooencyrtus nezarae, an egg parasitoid of Riptortus pedestris, using scanning electron microscopy. Male antennae was found to be significantly greater in overall length than female antennae. The antenna of O. Read More

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http://dx.doi.org/10.1002/jemt.23244DOI Listing
February 2019
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Effects of human umbilical cord mesenchymal stem cells on co-cultured ovarian carcinoma cells.

Microsc Res Tech 2019 Feb 20. Epub 2019 Feb 20.

Neurobiology Laboratory, Luohe Medical College, Luohe, China.

Ovarian carcinoma is mainly treated by surgery aided by chemotherapy. If supplemented by stem cells treatment, its recurrence rate and mortality rate will be decreased. This is a new therapy. Read More

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http://dx.doi.org/10.1002/jemt.23236DOI Listing
February 2019
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Evaluation of X-ray tomography contrast agents: A review of production, protocols, and biological applications.

Microsc Res Tech 2019 Feb 20. Epub 2019 Feb 20.

School of Engineering, London South Bank University, London, United Kingdom.

X-ray computed tomography is a strong tool that finds many applications both in medical applications and in the investigation of biological and nonbiological samples. In the clinics, X-ray tomography is widely used for diagnostic purposes whose three-dimensional imaging in high resolution helps physicians to obtain detailed image of investigated regions. Researchers in biological sciences and engineering use X-ray tomography because it is a nondestructive method to assess the structure of their samples. Read More

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http://dx.doi.org/10.1002/jemt.23225DOI Listing
February 2019
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The effect of final irrigation with MTAD, QMix, and EDTA on smear layer removal and mineral content of root canal dentin.

Microsc Res Tech 2019 Feb 20. Epub 2019 Feb 20.

Department of Histology and Embryology, Faculty of Medical Sciences, University of Kragujevac, Kragujevac, Serbia.

To compare the smear layer removal ability and mineral content of root canal dentine after initial irrigation with NaOCl and final irrigation with MTAD, QMix, and 17% EDTA. Forty extracted human maxillary incisors before root canal preparation and irrigation with NaOCl were randomly divided into four groups (n = 10) according to the type of final irrigants used: MTAD, QMix, 17% EDTA, and control (sterile distilled water). Scanning electron microscopy (SEM) was used to assess the presence of smear layer. Read More

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http://dx.doi.org/10.1002/jemt.23239DOI Listing
February 2019
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Modeling the clay minerals-enzyme binding by fusion fluorescent proteins and under atomic force microscope.

Microsc Res Tech 2019 Feb 18. Epub 2019 Feb 18.

Department of Agricultural Sciences, Southern University Agriculture Research and Extension Center, Baton Rouge, Louisiana.

In the present study, binding of cellulase protein to different clay minerals were tested using fluorescent-protein complex and microscopic techniques. Cellulase gene (Cel5H) was cloned into three fluorescent vectors and expressed as fusion enzymes. Binding of Cel5H-mineral particles was confirmed by confocal microscopy, and enzyme assay. Read More

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http://dx.doi.org/10.1002/jemt.23233DOI Listing
February 2019
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Cross-scale focus measure aggregation in depth recovery of microscopic objects.

Microsc Res Tech 2019 Feb 18. Epub 2019 Feb 18.

School of Computer Science and Engineering, Korea University of Technology and Education, Cheonan, 31253, Republic of Korea.

In shape from focus (SFF) methods, image focus volume enhancement is an important issue for acquiring accurate depth maps. Mostly, conventional approaches do focus aggregation locally to enhance the focus volume, which may not suppress noisy focus measurements properly and consequently, may provide deteriorated depth maps. Multiresolution fusion-based method is proposed for image focus volume enhancement. Read More

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http://dx.doi.org/10.1002/jemt.23230DOI Listing
February 2019
2 Reads

Feature enhancement framework for brain tumor segmentation and classification.

Microsc Res Tech 2019 Feb 15. Epub 2019 Feb 15.

Department of Computer Science, University of Engineering and Technology, Taxila, Pakistan.

Automatic medical image analysis is one of the key tasks being used by the medical community for disease diagnosis and treatment planning. Statistical methods are the major algorithms used and consist of few steps including preprocessing, feature extraction, segmentation, and classification. Performance of such statistical methods is an important factor for their successful adaptation. Read More

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http://dx.doi.org/10.1002/jemt.23224DOI Listing
February 2019
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Construction of saliency map and hybrid set of features for efficient segmentation and classification of skin lesion.

Microsc Res Tech 2019 Feb 15. Epub 2019 Feb 15.

Department of Information Systems, Al Yamamah University, Riyadh, KSA.

Skin cancer is being a most deadly type of cancers which have grown extensively worldwide from the last decade. For an accurate detection and classification of melanoma, several measures should be considered which include, contrast stretching, irregularity measurement, selection of most optimal features, and so forth. A poor contrast of lesion affects the segmentation accuracy and also increases classification error. Read More

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http://dx.doi.org/10.1002/jemt.23220DOI Listing
February 2019

Sample preparation method for visualization of nanoparticulate captured on mixed cellulose ester filter media by enhanced darkfield microscopy and hyperspectral imaging.

Microsc Res Tech 2019 Feb 15. Epub 2019 Feb 15.

College of Nanoscale Science, Nanobioscience Constellation, State University of New York (SUNY) Polytechnic Institute, College of Nanoscale Science, New York.

A significant hurdle in conducting effective health and safety hazard analysis and risk assessment for the nanotechnology workforce is the lack of a rapid method for the direct visualization and analysis of filter media used to sample nanomaterials from work environments that represent potential worker exposure. Current best-known methods include transmission electron microscopy (TEM) coupled with energy dispersive x-ray spectroscopy (EDS) for elemental identification. TEM-EDS is considerably time-, cost-, and resource-intensive, which may prevent timely health and safety recommendations and corrective actions. Read More

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http://dx.doi.org/10.1002/jemt.23231DOI Listing
February 2019
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Rapid histologic diagnosis using quick fluorescence staining and tissue confocal microscopy.

Microsc Res Tech 2019 Feb 14. Epub 2019 Feb 14.

Innovative Medical Engineering & Technology, National Cancer Center, Goyang, South Korea.

With the development of advanced and minimally invasive surgical techniques, and in view of the functional and cosmetic aspects, the need for rapid and accurate diagnosis during surgery is increasing. This study was conducted to develop a tissue diagnosis method using confocal microscopy after simple tissue staining that does not require freezing and slicing. At present, fluorescence staining with confocal microscopy is not generalized for real-time diagnosis during surgery. Read More

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http://doi.wiley.com/10.1002/jemt.23234
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http://dx.doi.org/10.1002/jemt.23234DOI Listing
February 2019
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Detoxification and functionalization of gold nanorods with organic polymers and their applications in cancer photothermal therapy.

Microsc Res Tech 2019 Feb 14. Epub 2019 Feb 14.

Institute of Applied Bioresource, College of Animal Sciences, Zhejiang University, Zhejiang, Hangzhou, People's Republic of China.

Gold nanorods (AuNRs) have attracted widespread attention in the last few decades due to their potential applications in cancer photothermal therapy (CPTT). However, the utilization of AuNRs is restricted because of their high toxicity for cells and tissues. Numerous methods have been developed to solve this problem, among which the most convenient one is to coat AuNRs with biocompatible materials or replace their original toxic surfactant such as the hexadecyl-trimethyl ammonium bromide (CTAB). Read More

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http://dx.doi.org/10.1002/jemt.23213DOI Listing
February 2019
1 Read