782,423 results match your criteria Microscopy and microanalysis : the official journal of Microscopy Society of America Microbeam Analysis Society Microscopical Society of Canada[Journal]


A simple layer-stacking technique to generate biomolecular and mechanical gradients in photocrosslinkable hydrogels.

Biofabrication 2019 Feb 20. Epub 2019 Feb 20.

Center for Minimally Invasive Therapeutics, UCLA, Los Angeles, California, UNITED STATES.

Physicochemical and biological gradients are desirable features for hydrogels to enhance their relevance to biological environments for three-dimensional (3D) cell culture. Therefore, simple and efficient techniques to generate chemical, physical and biological gradients within hydrogels are highly desirable. This work demonstrates a technique to generate biomolecular and mechanical gradients in photocrosslinkable hydrogels by stacking and crosslinking prehydrogel solution in a layer by layer manner. Read More

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http://dx.doi.org/10.1088/1758-5090/ab08b5DOI Listing
February 2019

How Good Can Single-Particle Cryo-EM Become? What Remains Before It Approaches Its Physical Limits?

Authors:
Robert M Glaeser

Annu Rev Biophys 2019 Feb 20. Epub 2019 Feb 20.

Molecular Biophysics and Integrated Bioimaging Division, Lawrence Berkeley National Laboratory, University of California, Berkeley, California 94720, USA; email:

Impressive though the achievements of single-particle cryo-electron microscopy are today, a substantial gap still remains between what is currently accomplished and what is theoretically possible. As is reviewed here, twofold or more improvements are possible as regards ( a) the detective quantum efficiency of cameras at high resolution, ( b) converting phase modulations to intensity modulations in the image, and ( c) recovering the full amount of high-resolution signal in the presence of beam-induced motion of the specimen. In addition, potential for improvement is reviewed for other topics such as optimal choice of electron energy, use of aberration correctors, and quantum metrology. Read More

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http://dx.doi.org/10.1146/annurev-biophys-070317-032828DOI Listing
February 2019

Surface Functionalization of Magnetite Nanoparticles with Sulfonic Acid and Heteropoly Acid: Efficient Magnetically Recoverable Solid Acid Catalysts.

Chem Asian J 2019 Feb 20. Epub 2019 Feb 20.

Faculty of Chemistry, Kharazmi University, Tehran, Islamic Republic of Iran.

New magnetically recoverable solid acid catalysts for acid-catalyzed reactions were designed via the surface chemical functionalization of silica-coated magnetite nanoparticles (SCMNPs) with sulfonic acid groups. First, the SCMNPs were covalently functionalized with 3-aminopropyl groups to achieve Amp-SCMNPs. Then, reaction of the Amp-SCMNPs with 1,4-butane sultone followed by acidification with phosphotungstic acid (HPW) or diluted sulfuric acid produced magnetically recoverable solid acid catalysts, HPW-ampsul-SCMNPs and H-ampsul-SCMNPs, respectively. Read More

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http://dx.doi.org/10.1002/asia.201801810DOI Listing
February 2019

The effect of final irrigation with MTAD, QMix, and EDTA on smear layer removal and mineral content of root canal dentin.

Microsc Res Tech 2019 Feb 20. Epub 2019 Feb 20.

Department of Histology and Embryology, Faculty of Medical Sciences, University of Kragujevac, Kragujevac, Serbia.

To compare the smear layer removal ability and mineral content of root canal dentine after initial irrigation with NaOCl and final irrigation with MTAD, QMix, and 17% EDTA. Forty extracted human maxillary incisors before root canal preparation and irrigation with NaOCl were randomly divided into four groups (n = 10) according to the type of final irrigants used: MTAD, QMix, 17% EDTA, and control (sterile distilled water). Scanning electron microscopy (SEM) was used to assess the presence of smear layer. Read More

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http://dx.doi.org/10.1002/jemt.23239DOI Listing
February 2019

The relationship between metastatic potential and in vitro mechanical properties of osteosarcoma cells.

Mol Biol Cell 2019 Feb 20:mbcE18080545. Epub 2019 Feb 20.

Biomechanics Laboratory, University Hospital Balgrist, University of Zürich, 8008 Zürich, Switzerland.

Osteosarcoma is the most frequent primary tumor of bone and is characterized by its high tendency to metastasize in lungs. Although in case of being early diagnosed treatment results in a 5-year survival rate of nearly 60%, prognosis of patients with secondary lesions at diagnosis is poor and their 5-year survival rate remains below 30%. In the present work we have used a number of analytical methods to investigate the impact of increased metastatic potential on the biophysical properties and force generation of osteosarcoma cells. Read More

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http://dx.doi.org/10.1091/mbc.E18-08-0545DOI Listing
February 2019

The activity of transient receptor potential channel C-6 modulates the differentiation of fat cells.

FASEB J 2019 Feb 20:fj201801518RR. Epub 2019 Feb 20.

School of Life Sciences, Faculty of Science, The Chinese University of Hong Kong, Hong Kong, China.

Previously, the V1-3 isoforms of the transient receptor potential channel (TRP) have been shown to promote or prevent adipocyte differentiation. In the current study, the C isoforms were screened for blocking adipogenesis. The hypothesis that the TRP classic or canonical (TRPC) deters adipocyte differentiation was investigated in 3T3-L1 cells employing the channel-specific activator and antagonist, silencing, and overexpression techniques. Read More

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http://dx.doi.org/10.1096/fj.201801518RRDOI Listing
February 2019

Experimental Evidence for a Cluster Glass Transition in Concentrated Lysozyme Solutions.

J Phys Chem B 2019 Feb 20. Epub 2019 Feb 20.

Lysozyme is known to form equilibrium clusters at a pH≈7.8 and at low ionic strength as a result of a mixed potential. While this cluster formation and the related dynamic and static structure factors have been extensively investigated, its consequences on the macroscopic dynamic behavior expressed by the zero shear viscosity η remain controversial. Read More

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http://dx.doi.org/10.1021/acs.jpcb.8b11781DOI Listing
February 2019

Mussel-inspired Self-healing Coatings Based on Polydopamine Coated Nanocontainers for Corrosion Protection.

ACS Appl Mater Interfaces 2019 Feb 20. Epub 2019 Feb 20.

Mussel-inspired properties of dopamine have attracted immense scientific interest for surface modification of nanoparticles due to the high potential of dopamine functional groups to increase adhesion of nanoparticles to flat surfaces. Here, we report for the first time a novel type of inhibitor-loaded nanocontainers using polydopamine (PDA) as a pH-sensitive gatekeeper for mesoporous silica nanoparticles (MSNs). The encapsulated inhibitor (benzotriazole) was loaded into MSNs at neutral pH, demonstrating fast release in acidic environment. Read More

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http://dx.doi.org/10.1021/acsami.8b21197DOI Listing
February 2019

Core-Shell Metal-Organic Frameworks As The Mixed-Mode Stationary Phase For Hydrophilic Interaction/Reversed-Phase Chromatography.

ACS Appl Mater Interfaces 2019 Feb 20. Epub 2019 Feb 20.

Stationary phases with mixed-mode mechanisms emerge as a hot research topic. In the present research, monodisperse core-shell UiO-67@SiO2 materials were prepared and further served as the packed column for mixed-mode hydrophilic interaction liquid chromatography/reversed-phase liquid chromatography. The developed UiO-67@SiO2 materials were characterized via thermogravimetric analysis, scanning electron microscopy, X Ray Powder diffraction as well as FT-IR techniques. Read More

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http://dx.doi.org/10.1021/acsami.9b00285DOI Listing
February 2019

Edge Segregated Polymorphism in 2D Molybdenum Carbide.

Adv Mater 2019 Feb 20:e1808343. Epub 2019 Feb 20.

Department of Chemistry, National University of Singapore, 3 Science Drive 3, Singapore, 17543, Singapore.

Molybdenum carbide (Mo C), a class of unterminated MXene, is endowed with rich polymorph chemistry, but the growth conditions of the various polymorphs are not understood. Other than the most commonly observed T-phase Mo C, little is known about other phases. Here, Mo C crystals are successfully grown consisting of mixed polymorphs and polytypes via a diffusion-mediated mechanism, using liquid copper as the diffusion barrier between the elemental precursors of Mo and C. Read More

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http://dx.doi.org/10.1002/adma.201808343DOI Listing
February 2019

Antitumoral effects of Amblyomma sculptum Berlese saliva in neuroblastoma cell lines involve cytoskeletal deconstruction and cell cycle arrest.

Rev Bras Parasitol Vet 2019 Feb 14. Epub 2019 Feb 14.

Programa de Ciências da Saúde, Pontifícia Universidade Católica do Paraná - PUCPR, Curitiba, PR, Brasil.

The antitumor properties of ticks salivary gland extracts or recombinant proteins have been reported recently, but little is known about the antitumor properties of the secreted components of saliva. The goal of this study was to investigate the in vitro effect of the saliva of the hard tick Amblyomma sculptum on neuroblastoma cell lines. SK-N-SK, SH-SY5Y, Be(2)-M17, IMR-32, and CHLA-20 cells were susceptible to saliva, with 80% reduction in their viability compared to untreated controls, as demonstrated by the methylene blue assay. Read More

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http://dx.doi.org/10.1590/S1984-296120180098DOI Listing
February 2019

Dermatitis in humans caused by Ornithonyssus bursa (Berlese 1888) (Mesostigmata: Macronyssidae) and new records from Brazil.

Rev Bras Parasitol Vet 2019 Feb 14. Epub 2019 Feb 14.

Departamento de Medicina Veterinária Preventiva e Saúde Animal, Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo - FMVZ-USP, São Paulo, SP, Brasil.

Ornithonyssus bursa, known as the "tropical fowl mite", is a hematophagous mite of domestic and wild birds, occasionally biting humans. Infestation on humans occurs mainly when the abandoned nests are close to homes, or by manipulation of infested birds by humans. In Brazil, this species occurs in the south and southeast of the country. Read More

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http://dx.doi.org/10.1590/S1984-296120180097DOI Listing
February 2019

Microscopic alterations in Fasciola hepatica from sheep treated with albendazole.

Rev Bras Parasitol Vet 2019 Feb 14. Epub 2019 Feb 14.

Departamento de Parasitologia Animal, Instituto de Veterinária, Universidade Federal Rural do Rio de Janeiro - UFRRJ, Seropédica, RJ, Brasil.

Currently, albendazole is one of the most commonly used drugs because of its affordability. The objective was to evaluate the histopathology of Fasciola hepatica specimens. For this, the efficacy test was performed on sheep treated with albendazole at the dose recommended for F. Read More

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http://dx.doi.org/10.1590/S1984-296120180095DOI Listing
February 2019

Protection of hepatocyte mitochondrial function by blueberry juice and probiotics via SIRT1 regulation in non-alcoholic fatty liver disease.

Food Funct 2019 Feb 20. Epub 2019 Feb 20.

Guiyang Dental Hospital, Guiyang 550003, China.

Mitochondrial dysfunction has been implicated in the pathogenesis of nonalcoholic fatty liver disease (NAFLD). Our previous study has firstly reported that blueberry juice and probiotics (BP) effectively protect liver function in NAFLD. However, the role of BP in hepatic mitochondria is unknown. Read More

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http://dx.doi.org/10.1039/c8fo02298dDOI Listing
February 2019

Stepwise activation mechanism of the scramblase nhTMEM16 revealed by cryo-EM.

Elife 2019 Feb 20;8. Epub 2019 Feb 20.

Department of Structural Biology, University of Groningen, Groningen, Netherlands.

Scramblases catalyze the movement of lipids between both leaflets of a bilayer. Whereas the X-ray structure of the protein nhTMEM16 has previously revealed the architecture of a Ca-dependent lipid scramblase, its regulation mechanism has remained elusive. Here, we have used cryo-electron microscopy and functional assays to address this question. Read More

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http://dx.doi.org/10.7554/eLife.44364DOI Listing
February 2019

Induction of Apoptosis in HeLa by Corn Small RNAs.

Nutr Cancer 2019 Feb 20:1-11. Epub 2019 Feb 20.

a Cancer and Translational Research Lab, Dr D. Y. Patil Biotechnology & Bioinformatics Institute, Dr D.Y. Patil Vidyapeeth , Pune , India.

Insights in RNA biology have opened up a plethora of opportunities to explore the small regulatory RNAs from various natural and artificial sources. These small RNAs have been suggested to play a role too in tumor progression by either as oncogenic or tumor suppressor small RNAs. In this study, authors have attempted to evaluate the therapeutic potential of small RNAs fractionated from corn (Zea mays) upon growth and survival of HeLa. Read More

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http://dx.doi.org/10.1080/01635581.2018.1526307DOI Listing
February 2019

Atomic-scale origin of enhanced ionic conductivity at crystal defects.

Nano Lett 2019 Feb 20. Epub 2019 Feb 20.

In oxide materials, the presence of dislocations often strongly affects the properties of thin film and multilayer devices. Although it was reported that ionic conduction can be enhanced by introducing dislocations in ionic conductors, the underlying mechanism of such enhancement remains unclear. Here we analyzed the ionic conduction enhancement in an yttria-stabilized zirconia (YSZ) single edge dislocation from structural point of view, using atomic resolution scanning transmission electron microscopy (STEM). Read More

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http://dx.doi.org/10.1021/acs.nanolett.9b00506DOI Listing
February 2019

Cryo-Electron Microscopy Uncovers Key Residues within the Core of Alpha-Synuclein Fibrils.

ACS Chem Neurosci 2019 Feb 20. Epub 2019 Feb 20.

Protein Folding and Dynamics Laboratory, Structural Biology and Bioinformatics Division , CSIR-Indian Institute of Chemical Biology , 4, Raja Subodh Chandra Mullick Road , Kolkata 700032 , India.

Recent expeditious advances in the determination of the 3-D structure of fibrils of alpha-synuclein, the intrinsically disordered protein associated with the neurodegenerative Parkinson's disease (PD), have identified amino acid contacts that form the fibril's inter-protofilament interface. The residues that constitute this "steric zipper" interface determine the morphology of the fibrils as well as toxicity of the oligomeric building units or "kernels" which lead to the formation of the protofilaments. The zipper interface houses key amino acid residues involved in familial PD that can be targeted by drug design. Read More

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http://dx.doi.org/10.1021/acschemneuro.9b00090DOI Listing
February 2019

Ferroelectric Properties of Perovskite Thin Films and Their Implications for Solar Energy Conversion.

Adv Mater 2019 Feb 20:e1806661. Epub 2019 Feb 20.

Karlsruhe Institute of Technology, Light Technology Institute (LTI), Engesserstrasse 13, 76131, Karlsruhe, Germany.

Whether or not methylammonium lead iodide (MAPbI ) is a ferroelectric semiconductor has caused controversy in the literature, fueled by many misunderstandings and imprecise definitions. Correlating recent literature reports and generic crystal properties with the authors' experimental evidence, the authors show that MAPbI thin-films are indeed semiconducting ferroelectrics and exhibit spontaneous polarization upon transition from the cubic high-temperature phase to the tetragonal phase at room temperature. The polarization is predominantly oriented in-plane and is organized in characteristic domains as probed with piezoresponse force microscopy. Read More

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http://dx.doi.org/10.1002/adma.201806661DOI Listing
February 2019

Antimicrobial and anti-biofilm properties of novel synthetic lignan-like compounds.

New Microbiol 2019 Feb 20;42(1). Epub 2019 Feb 20.

Department of Pharmacy, University of Naples Federico II, 80131, Italy.

Antibiotic resistance and biofilm tolerance are among the principal factors involved in the persistence of chronic infections. The need for new antimicrobials is an ever-increasing challenge in clinical environments and in the control of global health. Arylfurans form a set of structures that have been identified in many natural products, e. Read More

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February 2019

Zein-based core-shell microcapsules for the potential delivery of algae oil and lipophilic compounds.

Food Funct 2019 Feb 20. Epub 2019 Feb 20.

Protein Research and Development Center, Guangdong Province Key Laboratory for Green Processing of Natural Products and Product Safety, National Engineering Laboratory of Wheat & Corn Further Processing, South China University of Technology, Guangzhou 510640, P. R China.

Due to the benefits involving brain development and cardiovascular health, algae oil which is rich in omega-3 fatty acids has become a popular dietary supplement in recent years. However, the incompatibility with water and poor oxidative stability limits its addition in foods and beverages. In this work, the formation and characterization of zein-based core-shell microcapsules with tunable shell thicknesses for the potential delivery of algae oil were reported. Read More

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http://dx.doi.org/10.1039/c8fo02302fDOI Listing
February 2019

Lipid coated liquid crystal droplets for the on-chip detection of antimicrobial peptides.

Lab Chip 2019 Feb 20. Epub 2019 Feb 20.

School of Physics and Astronomy, University of Leeds, Leeds, UK.

We describe a novel biosensor based on phospholipid-coated nematic liquid crystal (LC) droplets and demonstrate the detection of Smp43, a model antimicrobial peptide (AMP) from the venom of North African scorpion Scorpio maurus palmatus. Mono-disperse lipid-coated LC droplets of diameter 16.7 ± 0. Read More

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http://dx.doi.org/10.1039/c8lc01291aDOI Listing
February 2019

Cationic polymer-derived carbon dots for enhanced gene delivery and cell imaging.

Biomater Sci 2019 Feb 20. Epub 2019 Feb 20.

Key Laboratory of Green Chemistry and Technology (Ministry of Education), College of Chemistry, Sichuan University, Chengdu 610064, P. R. China.

Carbon dots have attracted rapidly growing interest in recent years. In this report, we prepared two cationic polymer-derived carbon dots (Taea-CD and Cyclen-CD, collectively called C-dots) via a hydrothermal method. Transmission electron microscopy (TEM) results show that the C-dots were sphere-like and the size distribution was 1. Read More

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http://dx.doi.org/10.1039/c8bm01578cDOI Listing
February 2019

Antitumor Activity of Asiaticoside Against Multiple Myeloma Drug-Resistant Cancer Cells Is Mediated by Autophagy Induction, Activation of Effector Caspases, and Inhibition of Cell Migration, Invasion, and STAT-3 Signaling Pathway.

Med Sci Monit 2019 Feb 20;25:1355-1361. Epub 2019 Feb 20.

Department of Hematology, Shengjing Hospital, China Medical University, Shenyang, Liaoning, China (mainland).

BACKGROUND Accumulating evidence suggests that plant-derived molecules may prove extremely beneficial in the development of chemotherapy for deadly cancer types. Multiple myeloma is a rare and incurable type of cancers. Very little research has been directed towards the development of chemotherapy for the management of multiple myeloma. Read More

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http://dx.doi.org/10.12659/MSM.913397DOI Listing
February 2019

Determination of norfloxacin in urine and pharmaceutical samples using terbium doped zinc sulphide nanomaterials-sensitized fluorescence method.

Spectrochim Acta A Mol Biomol Spectrosc 2019 Feb 12;214:261-268. Epub 2019 Feb 12.

Department of Chemistry, Punjabi University, Patiala 147002, Punjab, India. Electronic address:

Highly crystalline polyethylene glycol (PEG) coated Tb doped ZnS nanoparticles have been synthesized and successfully used for norfloxacin sensing. The crystallographic and morphological analyses of PEG coated Tb doped ZnS nanoparticles were performed by X-ray diffraction and Transmission electron microscopy, respectively. The confirmation of Tb doping in ZnS host matrix was done by emission spectroscopy and energy dispersive X-ray spectroscopy. Read More

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http://dx.doi.org/10.1016/j.saa.2019.02.015DOI Listing
February 2019

Food additive dye (quinoline yellow) promotes unfolding and aggregation of myoglobin: A spectroscopic and molecular docking analysis.

Spectrochim Acta A Mol Biomol Spectrosc 2019 Feb 13;214:216-226. Epub 2019 Feb 13.

Department of Chemistry, Collage of Science Princess Noura Bint Abdul Rahman University, Riyadh, Saudi Arabia. Electronic address:

Protein aggregation leads to vast conformational changes and plays a key role in the pathogenesis of various neurodegenerative diseases including Alzheimer's and Parkinson's. In the current piece of work, we have explored the interaction of quinoline yellow (QY) with myoglobin (Mb) at two different pH (3.5 and 7. Read More

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http://dx.doi.org/10.1016/j.saa.2019.01.090DOI Listing
February 2019

Spatiotemporally Controlled Myosin Relocalization and Internal Pressure Generate Sibling Cell Size Asymmetry.

iScience 2019 Feb 6;13:9-19. Epub 2019 Feb 6.

Department of Biology, University of Washington, Life Science Building, Seattle, WA 98195, USA. Electronic address:

Metazoan cells can generate unequal-sized sibling cells during cell division. This form of asymmetric cell division depends on spindle geometry and Myosin distribution, but the underlying mechanics are unclear. Here, we use atomic force microscopy and live cell imaging to elucidate the biophysical forces involved in the establishment of physical asymmetry in Drosophila neural stem cells. Read More

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http://dx.doi.org/10.1016/j.isci.2019.02.002DOI Listing
February 2019

Novel nanocomposite derived from ZnO/CdS QDs embedded crosslinked chitosan: An efficient photocatalyst and effective antibacterial agent.

J Hazard Mater 2019 Feb 10;369:398-407. Epub 2019 Feb 10.

Department of Applied Chemistry, Indian Institute of Technology (ISM), Dhanbad, 826004, India. Electronic address:

A novel nanocomposite (cl-Ch-pMAc@ZnO/CdSQDs) has been developed under microwave irradiation via fabrication of ZnO/CdS quantum dots on anionically functionalized chitosan [i.e. poly (methacrylic acid) crosslinked chitosan (cl-Ch-pMAc) in the presence of diethylene glycol dimethacrylate (DEGDMA) crosslinker]. Read More

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http://dx.doi.org/10.1016/j.jhazmat.2019.02.022DOI Listing
February 2019

A novel L-Histidine (C, N) codoped-TiO-CdS nanocomposite for efficient visible photo-degradation of recalcitrant compounds from wastewater.

J Hazard Mater 2019 Feb 14;369:384-397. Epub 2019 Feb 14.

Institut fuer Technische Chemie, Leibniz Universität Hannover, Callinstrasse 3, D-30167 Hannover, Germany.

The aim of current study is to synthesis novel visible driven photocatalysts (L-Histidine (C, N) codoped-TiO-CdS) with different loadings of L-Hisitdine (1, 2, and 3 wt.%) and CdS (1:9, 7:1, and 1:5 mass ratios of CdS to TiO). Then, their application for photo-degradation of methyl orange (MO) and biologically treated palm oil mill effluent (POME) were studied. Read More

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http://dx.doi.org/10.1016/j.jhazmat.2019.02.049DOI Listing
February 2019

Evaluation of the sub-surface morphology and composition of gunshot residue using focussed ion beam analysis.

Forensic Sci Int 2019 Feb 11;297:100-110. Epub 2019 Feb 11.

College of Science and Engineering, Flinders University, Sturt Road, Bedford Park, South Australia, Australia.

Recent work in the forensic analysis of Gunshot residues (GSR) has suggested that the sub-surface or internal composition and morphology of these residues be explored. A particular area of interest is in heavy metal free, or non-toxic ammunition, which are becoming more frequently encountered in the marketplace. As the formulation of the primer compound changes the conditions of the firearm discharge, there is the possibility that different primer formulations may result in the formation of different GSR particles with distinct internal morphologies and compositions. Read More

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http://dx.doi.org/10.1016/j.forsciint.2019.01.030DOI Listing
February 2019

Alkaloids from Nelumbinis Plumula (AFNP) ameliorate aortic remodeling via RhoA/ROCK pathway.

Biomed Pharmacother 2019 Feb 20;112:108651. Epub 2019 Feb 20.

Academy of Integrative Medicine, Fujian University of Traditional Chinese Medicine, Fuzhou, Fujian, 350122, PR China; Fujian Key Laboratory of Integrative Medicine on Geriatrics, Fujian University of Traditional Chinese Medicine, Fuzhou, Fujian, 350122, PR China. Electronic address:

The protective role of alkaloids from Nelumbinis Plumula (AFNP) on the aorta during hypertension is not yet fully understood. We hypothesize that AFNP exerts protective effects against Ang II-induced hypertension by mediating RhoA/ROCK pathway and phenotypic switching during hypertension. In the present study, we evaluated the effect of AFNP on angiotensin II (Ang II)-induced actin cytoskeleton reorganization and aorta remodeling, as well as the involvement of RhoA/Rho-associated coiled kinase (ROCK) pathway in protecting against hypertension. Read More

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http://dx.doi.org/10.1016/j.biopha.2019.108651DOI Listing
February 2019

Opuntioside, opuntiol and its metallic nanoparticles attenuate adjuvant-induced arthritis: Novel suppressors of Toll-like receptors -2 and -4.

Biomed Pharmacother 2019 Feb 20;112:108624. Epub 2019 Feb 20.

HEJ Research Institute of Chemistry, International Center for Chemical and Biological Sciences, University of Karachi, Karachi, 74270, Pakistan. Electronic address:

Rheumatoid arthritis (RA) is a chronic autoimmune disease of synovial inflammation and joint destruction. This study reports anti-arthritic potential of opuntioside-I opuntiol, and its gold and silver nanoparticles (NPs) against Complete Freund's Adjuvant (CFA)-induced arthritic rats. The mechanistic studies were performed targeting TLRs (TLR-2 and TLR-4) and cytokines (IL-1β and TNF-α) expressions to validate their anti-inflammatory and immuno-modulatory response. Read More

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http://dx.doi.org/10.1016/j.biopha.2019.108624DOI Listing
February 2019
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Design and development of multitarget-directed N-Benzylpiperidine analogs as potential candidates for the treatment of Alzheimer's disease.

Eur J Med Chem 2019 Feb 14;167:510-524. Epub 2019 Feb 14.

Department of Pharmaceutical Engineering & Technology, Indian Institute of Technology (Banaras Hindu University), Varanasi, 221005, India. Electronic address:

The multitarget-directed strategy offers an effective and promising paradigm to treat the complex neurodegenerative disorder, such as Alzheimer's disease (AD). Herein, a series of N-benzylpiperidine analogs (17-31 and 32-46) were designed and synthesized as multi-functional inhibitors of acetylcholinesterase (AChE) and β-secretase-1 (BACE-1) with moderate to excellent inhibitory activities. Among the tested inhibitors, 25, 26, 40, and 41 presented the most significant and balanced inhibition against both the targets. Read More

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http://dx.doi.org/10.1016/j.ejmech.2019.02.030DOI Listing
February 2019

Detectability of the degree of freeze damage in meat depends on analytic-tool selection.

Meat Sci 2019 Feb 8;152:8-19. Epub 2019 Feb 8.

Faculty of Ecology and Natural Resource Management, Norwegian University of Life Sciences, 1432 Aas, Norway.

Novel freezing solutions are constantly being developed to reduce quality loss in meat production chains. However, there is limited focus on identifying the sensitive analytical tools needed to directly validate product changes that result from potential improvements in freezing technology. To benchmark analytical tools relevant to meat research and production, we froze pork samples using traditional (-25 °C, -35 °C) and cryogenic freezing (-196 °C). Read More

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http://dx.doi.org/10.1016/j.meatsci.2019.02.002DOI Listing
February 2019

Effects of miR-181a-5p abnormal expression on zebrafish (Danio rerio) vascular development following triclosan exposure.

Chemosphere 2019 Feb 11;223:523-535. Epub 2019 Feb 11.

Zhejiang Provincial Key Laboratory of Medical Genetics, Key Laboratory of Laboratory Medicine, Ministry of Education, China, School of Laboratory Medicine and Life Sciences, Wenzhou Medical University, Wenzhou, Zhejiang, 325035, China. Electronic address:

Triclosan (TCS), one of the important bactericides, is widely used in personal care products, and its chronic exposure leads to severe toxic effects on the growth and development of blood vessels in zebrafish (Danio rerio). Herein, we screened out three differentially expressed miRNAs (miR-181a-5p, miR-132-3p and miR-128-3p) by sequencing and qRT-PCR analyses of 4-96-hpf TCS-exposed zebrafish, among which miR-181a-5p was found to regulate many signaling pathways involved in fatty acid biosynthesis and phosphatidylimositol signaling systems. By O-dianisidine staining, TCS-exposure resulted in decreased distribution of red blood cells and induced blood hypercoagulable state and thrombotic effects. Read More

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http://dx.doi.org/10.1016/j.chemosphere.2019.02.038DOI Listing
February 2019

Filamentous Aggregates Are Fragmented by the Proteasome Holoenzyme.

Cell Rep 2019 Feb;26(8):2140-2149.e3

Department of Chemistry, University of Cambridge, Lensfield Road, Cambridge CB2 1EW, UK; Department of Cell Biology, Harvard Medical School, Longwood Avenue, Boston, MA 02115, USA. Electronic address:

Filamentous aggregates (fibrils) are regarded as the final stage in the assembly of amyloidogenic proteins and are formed in many neurodegenerative diseases. Accumulation of aggregates occurs as a result of an imbalance between their formation and removal. Here we use single-aggregate imaging to show that large fibrils assembled from full-length tau are substrates of the 26S proteasome holoenzyme, which fragments them into small aggregates. Read More

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http://dx.doi.org/10.1016/j.celrep.2019.01.096DOI Listing
February 2019

Facile synthesis of graphitic carbon-nitride supported antimony-doped tin oxide nanocomposite and its application for the adsorption of volatile organic compounds.

J Environ Sci (China) 2019 May 30;79:35-42. Epub 2018 Oct 30.

Department of Convergence Technology Engineering, Chonbuk National University, Jeonju 561-756, Republic of Korea; Eco-friendly Machine Parts Design Center, Chonbuk National University, Jeonju 561-756, Republic of Korea. Electronic address:

Antimony-doped tin oxide (ATO) nanoparticles with an average size of ~ 6 nm were prepared by co-precipitation and subsequent heat treatment. Graphitic carbon nitride (g-CN)/ATO hybrid nanocomposite was designed by the combination of thermally synthesized g-CN and ATO nanoparticles by ultrasonication. The materials were characterized using N adsorption/desorption (BET), X-ray diffraction (XRD), scanning electron microscopy (SEM), energy dispersive spectroscopy (EDS), transmission electron microscopy (TEM) and Fourier transform infrared spectroscopy (FTIR). Read More

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http://dx.doi.org/10.1016/j.jes.2018.10.008DOI Listing

Visible-light driven photocatalytic degradation of bisphenol-A using ultrasonically synthesized polypyrrole/K-birnessite nanohybrids: Experimental and DFT studies.

J Environ Sci (China) 2019 May 13;79:161-173. Epub 2018 Dec 13.

Materials Research Laboratory, Department of Chemistry, Jamia Millia Islamia, New Delhi 110025, India. Electronic address:

Although manganese oxides are known for their semiconductor characteristics, the photocatalytic performance of conducting polymer intercalated K-Birnessite (K-Bi) has not been explored till date. With the view to design a visible light driven organic-inorganic hybrid photocatalyst for rapid degradation of Bisphenol A (BPA), the present work reports the ultrasound-assisted green synthesis of K-Bi/polypyrrole (Ppy) nanohybrids. The loading of Ppy in K-Bi was confirmed by thermogravimetric analysis while the formation of organic-inorganic hybrid was confirmed by infrared spectroscopy. Read More

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http://dx.doi.org/10.1016/j.jes.2018.11.021DOI Listing

DNA binding adaptors to assemble proteins of interest on DNA scaffold.

Methods Enzymol 2019 13;617:287-322. Epub 2019 Feb 13.

Institute of Advanced Energy, Kyoto University, Uji, Kyoto, Japan. Electronic address:

DNA nanostructures serve as the ideal scaffolds to assemble materials of interest. Among these, proteins are of particularly interesting class of molecules to assemble because of their huge functional variability. Sequence-specific DNA binding proteins have been applied as adaptors to stably locate the fused proteins at defined positions of DNA scaffold in high loading yields. Read More

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http://dx.doi.org/10.1016/bs.mie.2018.12.014DOI Listing
February 2019

Cargo encapsulation in bacterial microcompartments: Methods and analysis.

Methods Enzymol 2019 10;617:155-186. Epub 2019 Feb 10.

Department of Chemical and Biological Engineering, Northwestern University, Technological Institute, Evanston, IL, United States; Center for Synthetic Biology, Northwestern University, Technological Institute, Evanston, IL, United States. Electronic address:

Metabolic engineers seek to produce high-value products from inexpensive starting materials in a sustainable and cost-effective manner by using microbes as cellular factories. However, pathway development and optimization can be arduous tasks, complicated by pathway bottlenecks and toxicity. Pathway organization has emerged as a potential solution to these issues, and the use of protein- or DNA-based scaffolds has successfully increased the production of several industrially relevant compounds. Read More

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http://dx.doi.org/10.1016/bs.mie.2018.12.009DOI Listing
February 2019

[Express] Role of Intraganglionic Transmission in the Trigeminovascular Pathway.

Mol Pain 2019 Feb 20:1744806919836570. Epub 2019 Feb 20.

Indiana University School of Medicine.

Migraine is triggered by poor air quality and odors through unknown mechanisms. Activation of the trigeminovascular pathway by environmental irritants may occur via activation of TRPA1 receptors on nasal trigeminal neurons, but how that results in peripheral and central sensitization is unclear. The anatomy of the trigeminal ganglion suggests that noxious nasal stimuli are not being transduced to the meninges by axon reflex but likely through intraganglionic transmission. Read More

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http://dx.doi.org/10.1177/1744806919836570DOI Listing
February 2019

Spherical agglomeration to improve dissolution and micromeritic properties of an anticancer drug, Bicalutamide.

Drug Dev Ind Pharm 2019 Feb 20:1-40. Epub 2019 Feb 20.

a Department of Pharmaceutics , ROFEL, Shri G. M. Bilakhia College of Pharmacy , Vapi , 396191 , India.

Bicalutamide (BCT), an anticancer drug, suffers from dissolution rate limited bioavailability and poor micromeritic properties. Spherical crystallization involves formation of spherical agglomerates with enhanced dissolution properties, obviating the need of further granulation process. The present investigation was focused on spherical agglomeration of BCT by quasi emulsion solvent diffusion method. Read More

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http://dx.doi.org/10.1080/03639045.2019.1585447DOI Listing
February 2019

In vitro and in vivo characterization of pharmaceutical nanocarriers used for drug delivery.

Artif Cells Nanomed Biotechnol 2019 Dec;47(1):524-539

a Institute of Pharmaceutical Sciences , Guru Ghasidas Vishwavidyalaya , Bilaspur , Chhattisgarh , India.

Nanotechnology has emerged strongly in most of the field of sciences at a tiny scale. At this size, atoms and molecules work differently and present a diversity of amazing and appealing applications. Pharmaceutical nanocarriers comprise nanoparticles, nanospheres, nanocapsules, nanoemulsion, nanoliposomes and nanoniosomes. Read More

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http://dx.doi.org/10.1080/21691401.2018.1561457DOI Listing
December 2019

Novel dye for detection of callus embryo by confocal laser scanning fluorescence microscopy.

Luminescence 2019 Feb 19. Epub 2019 Feb 19.

Department of Biotechnology, Daugavpils University, Institute of Life Sciences and Technology, Daugavpils, Latvia.

In the present study a new luminescent dye 3-N-(2-pyrrolidinylacetamido)benzanthrone (AZR) was synthesized. Spectroscopic measurements of the novel benzanthrone 3-aminoderivative were performed in seven organic solvents showing strong fluorescence. The capability of the prepared dye for visualization has been tested on flax, red clover and alfalfa to determinate the embryo in plant callus tissue cultures. Read More

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http://dx.doi.org/10.1002/bio.3616DOI Listing
February 2019

Gel- and Solid-State-Structure of Dialanine and Diphenylalanine Amphiphiles - The importance of C∙∙∙H Interactions in Gelation.

Chemphyschem 2019 Feb 19. Epub 2019 Feb 19.

University of New South Wales, School of Chemistry, School of Chemistry, 2052, Sydney, AUSTRALIA.

To investigate the role of the capping group in the solution and solid-state self-assembly of short peptide amphiphiles, dialanine and diphenylalanine have been linked via the N-terminus to a benzene (phenyl) and 3-naphthyl capping groups using five different methylene linkers; (CH₂)n, n = 0-4 for the benezene and three (0, 1 and 2) for the naphthalene capping group. Atomic force microscopy (AFM), oscillatory rheology, circular dichroism (CD) and IR analysis have been employed to understand the properties of these peptide-based hydrogels. Several X-ray structures of these short peptide gelators give useful conformational information regarding packing. Read More

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http://dx.doi.org/10.1002/cphc.201801104DOI Listing
February 2019

Ultrasound microscopy through a fine fiber for renal tissues.

Clin Exp Nephrol 2019 Feb 19. Epub 2019 Feb 19.

Department of Pediatrics, Jichi Medical University, 3311-1 Yakushiji, Shimotsuke, Tochigi, 329-0498, Japan.

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http://dx.doi.org/10.1007/s10157-019-01703-yDOI Listing
February 2019

Formate-removing inoculum dominated by Methanobacterium congolense supports succinate production from crude glycerol fermentation.

J Ind Microbiol Biotechnol 2019 Feb 19. Epub 2019 Feb 19.

Department of Life Science, Ewha Womans University, 52, Ewhayeodae-gil, Seodaemun-gu, Seoul, 03760, Republic of Korea.

We developed a formate-removing methanogenic inoculum (FRI) to facilitate succinate production from crude glycerol by Escherichia coli. FRI converted formate to methane, thereby enabling glycerol fermentation without additional electron acceptors under neutral pH. FRI was selectively enriched from sludge from the anaerobic digester of the Seonam sewage treatment plant (Seoul); this process was assessed via Illumina sequencing and scanning electron microscopy imaging. Read More

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http://dx.doi.org/10.1007/s10295-019-02154-wDOI Listing
February 2019

Exosomes derived from mesenchymal stem cells inhibit mitochondrial dysfunction-induced apoptosis of chondrocytes via p38, ERK, and Akt pathways.

In Vitro Cell Dev Biol Anim 2019 Feb 19. Epub 2019 Feb 19.

Beijing Research Institute of Traumatology and Orthopaedics, Beijing Jishuitan Hospital, Beijing, 100035, China.

Osteoarthritis (OA) is the most common chronic joint disease worldwide. Chondrocyte, as the only resident cell type in cartilage, its apoptosis is of pathogenetic significance in OA. Mesenchymal stem cell (MSC)-based-therapy has been proved effective in OA in animals and clinical studies. Read More

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http://dx.doi.org/10.1007/s11626-019-00330-xDOI Listing
February 2019

2D-Single-crystal hexagonal gold nanosheets for ultra-trace voltammetric determination of captopril.

Mikrochim Acta 2019 Feb 19;186(3):195. Epub 2019 Feb 19.

Analytical Research Laboratory, Department of Chemistry, University of Sistan and Baluchestan, P.O. Box 98135-674, Zahedan, 98167-45845, Iran.

Two dimensional single-crystal hexagonal gold nanosheets (SCHGNSs) were prepared by microwave heating of a solution of HAuCl in an ionic liquid. The SCHGNSs were characterized by field emission electron microscopy, energy-dispersive X-ray spectroscopy, X-ray diffraction, atomic force microscopy and electrochemical impedance spectroscopy. The SCHGNSs were then used to modify a graphite paste electrode for voltammetric determination of the hypertension drug captopril (CAP). Read More

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http://dx.doi.org/10.1007/s00604-019-3260-9DOI Listing
February 2019

A computational fluid dynamics approach to determine white matter permeability.

Biomech Model Mechanobiol 2019 Feb 20. Epub 2019 Feb 20.

Department of Mechanical Engineering, Imperial College London, London, SW7 2AZ, UK.

Glioblastomas represent a challenging problem with an extremely poor survival rate. Since these tumour cells have a highly invasive character, an effective surgical resection as well as chemotherapy and radiotherapy is very difficult. Convection-enhanced delivery (CED), a technique that consists in the injection of a therapeutic agent directly into the parenchyma, has shown encouraging results. Read More

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http://dx.doi.org/10.1007/s10237-019-01131-7DOI Listing
February 2019
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