788,890 results match your criteria Microscopy and microanalysis : the official journal of Microscopy Society of America Microbeam Analysis Society Microscopical Society of Canada[Journal]


Folding the genome into mitotic chromosomes.

Curr Opin Cell Biol 2019 Apr 15;60:19-26. Epub 2019 Apr 15.

Division of Experimental Pathology, Cancer Institute of the Japanese Foundation for Cancer Research (JFCR), Tokyo, Japan. Electronic address:

How linear DNA molecules are packaged into compact cylindrical chromosomes in preparation for cell division has remained one of the central outstanding questions in cell biology. Condensin is a highly conserved protein complex that universally determines large-scale DNA geometry during mitotic chromosome assembly. A wide range of recently developed approaches, including super resolution microscopy, single molecule imaging, Hi-C analyses and computational modeling, have profoundly changed how we view mitotic chromosomes. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S09550674193002
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http://dx.doi.org/10.1016/j.ceb.2019.03.005DOI Listing
April 2019
1 Read

Cluster binding studies with two anti-Thomsen-Friedenreich (anti-core-1, CD176, TF) antibodies: Evidence for a multiple TF epitope.

Int Immunopharmacol 2019 Apr 15;72:186-194. Epub 2019 Apr 15.

Glycotope GmbH, 13125 Berlin-Buch, Germany. Electronic address:

Antibodies to carbohydrate epitopes are often of the IgM isotype and require multiple binding for sufficient avidity. Therefore clusters of epitopes are preferred antigenic sites in these cases. We have examined the type of clusters recognized by two anti-Thomsen-Friedenreich (TF, core-1, CD176) IgM antibodies, NM-TF1 and NM-TF2, using several different sets of TF-carrying synthetic glycoconjugates in ELISA experiments. Read More

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http://dx.doi.org/10.1016/j.intimp.2019.03.058DOI Listing

Evaluation of the genotoxicity of neurotoxic insecticides using the micronucleus test in Tradescantia pallida.

Chemosphere 2019 Apr 12;227:371-380. Epub 2019 Apr 12.

Institute of Biotechnology, Federal University of Uberlândia, Campus Umuarama, 38900-402, Uberlândia, Minas Gerais, Brazil.

Neonicotinoids and phenylpyrazoles are classes of neurotoxic insecticides which are able to bind at different ligand sites of neural receptors, leading to the deregulation of insect neural activity and hence resulting in death. The misuse or indiscriminate use of these chemicals is directly associated with several toxicological effects in biota and at different trophic levels. Based on this premise, the aim of the present study was to evaluate and compare the genotoxic capacity of different concentrations of thiamethoxam (TMX), acetamiprid (ACP), imidacloprid (IMI) and fipronil (FP) through the Micronucleus Test in Tradescantia pallida (Trad-MCN). Read More

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https://linkinghub.elsevier.com/retrieve/pii/S00456535193072
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http://dx.doi.org/10.1016/j.chemosphere.2019.04.073DOI Listing
April 2019
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Superparamagnetic iron oxide nanoparticles of curcumin enhance gemcitabine therapeutic response in pancreatic cancer.

Biomaterials 2019 Apr 8;208:83-97. Epub 2019 Apr 8.

Department of Pharmaceutical Sciences and Center for Cancer Research, University of Tennessee Health Science Center, Memphis, USA. Electronic address:

Pancreatic cancer is a complex disease accounting for fibrotic tumors and an aggressive phenotype. Gemcitabine (GEM) is used as a standard therapy, which develops chemoresistance leading to poor patient outcome. We have recently developed a superparamagnetic iron oxide nanoparticle (SPION) formulation of curcumin (SP-CUR), which is a nontoxic, bioactive anti-inflammatory/anti-cancer agent for its enhanced delivery in tumors. Read More

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http://dx.doi.org/10.1016/j.biomaterials.2019.04.005DOI Listing

A new test system for unraveling the effects of soil components on the uptake and toxicity of silver nanoparticles (NM-300K) in simulated pore water.

Sci Total Environ 2019 Apr 9;673:613-621. Epub 2019 Apr 9.

University of Bremen, FB02, UFT, General and Theoretical Ecology, Leobener Str. 6, 28359 Bremen, Germany.

Fate, bioavailability and toxicity of silver nanoparticles (AgNP) are largely affected by soil properties. Here we focused on how these processes are connected in simulated soil pore water. OECD soil components (sand, kaolin clay, peat) were covered with NM-300K-, AgNO- and NM-300K dispersant-contaminated water, and Folsomia candida were exposed on the water surface. Read More

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http://dx.doi.org/10.1016/j.scitotenv.2019.03.493DOI Listing

Developing biocompatible silver nanoparticles using epigallocatechin gallate for dental use.

Arch Oral Biol 2019 Mar 28;102:106-112. Epub 2019 Mar 28.

Faculty of Dentistry, The University of Hong Kong, Hong Kong, China. Electronic address:

Objective: To develop silver nanoparticles (AgNPs) using epigallocatechin gallate (EGCG) and evaluate its biocompatibility and inhibition effect on Streptococcus mutans biofilm growth.

Design: AgNPs were synthesized using EGCG as a reducing agent. Cytotoxicity was assessed using half-maximal inhibitory concentration (IC) against human gingival fibroblast (HGF-1) and stem cells from human exfoliated deciduous teeth (SHED). Read More

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http://dx.doi.org/10.1016/j.archoralbio.2019.03.022DOI Listing

Inhalable co-amorphous budesonide-arginine dry powders prepared by spray drying.

Int J Pharm 2019 Apr 15. Epub 2019 Apr 15.

Department of Pharmacy, University of Copenhagen, Universitetsparken 2, DK-2100, Copenhagen, Denmark; Wuya College of Innovation, Shenyang Pharmaceutical University, Wenhua Road 103, 110016, Shenyang, China. Electronic address:

Spray drying is a well-established technology to produce inhalable dry powders. However, the amorphous nature of the particles typically obtained from the process can lead to physically and chemically unstable products. The purpose of this study was to investigate whether spray-drying could be used as a manufacturing method to produce co-amorphous drug amino acid powders with high physical stability and inhalable particulate properties. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S03785173193029
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http://dx.doi.org/10.1016/j.ijpharm.2019.04.036DOI Listing
April 2019
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Egg white coated alginate nanoparticles with electron sprayer for potential anticancer application.

Int J Pharm 2019 Apr 15. Epub 2019 Apr 15.

Research Institute of Sun Yat-Sen University in Shenzhen, Shenzhen, 518057, PR China; Key Laboratory of Sensing Technology and Biomedical Instrument of Guangdong Province, School of Biomedical Engineering, Sun Yat-sen University, Guangzhou 510006, PR China. Electronic address:

To development biomimetic strategy for enhanced cancer therapy, the paclitaxel loaded egg white/sodium alginate nanoparticles were prepared by electronic spray method. Their appearance and particle size were observed by transmission electron microscope and particle size analyzer. The drug release behavior of nanoparticles was determined by high performance liquid chromatography (HPLC). Read More

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http://dx.doi.org/10.1016/j.ijpharm.2019.04.045DOI Listing

Post-irradiation recovery time strongly influences fractional laser-facilitated skin absorption.

Int J Pharm 2019 Apr 15. Epub 2019 Apr 15.

Research Center for Food and Cosmetic Safety and Research Center for Chinese Herbal Medicine, Chang Gung University of Science and Technology, Kweishan, Taoyuan, Taiwan; Pharmaceutics Laboratory, Graduate Institute of Natural Products, Chang Gung University, Kweishan, Taoyuan, Taiwan; Department of Anesthesiology, Chang Gung Memorial Hospital, Kweishan, Taoyuan, Taiwan. Electronic address:

Fractional CO laser treatment has been used in some clinical trials to promote topical drug delivery. Currently, there is no standard for laser settings to achieve a feasible therapy. The cutaneous recovery following laser treatment and its influence on drug absorption have not been well explored. Read More

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http://dx.doi.org/10.1016/j.ijpharm.2019.04.043DOI Listing

The nanoscopic molecular pathway through human skin.

Biochim Biophys Acta Gen Subj 2019 Apr 15. Epub 2019 Apr 15.

Department of Biochemistry and Molecular Biology, University of Southern Denmark, Denmark. Electronic address:

Background: Knowledge regarding the barrier properties of human skin is important for understanding skin pathology, developing of transdermal drug delivery systems and computational skin absorption models; however, the molecular pathways through human skin remains to be fully investigated on a nanoscopic level. In particular the nanoscopic pathway of molecules passing the intercellular lipid bilayers separating the corneocytes in the stratum corneum (SC) is not fully elucidated.

Methods: Using stimulated emission depletion microscopy (STED) and Förster resonance energy transfer (FRET) the molecular pathways through the SC, the main barrier of the skin, are determined for lipophilic and water-soluble molecules at a nanoscopic resolution. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S03044165193008
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http://dx.doi.org/10.1016/j.bbagen.2019.04.012DOI Listing

Quantitative atomic force microscopy provides new insight into matrix vesicle mineralization.

Arch Biochem Biophys 2019 Apr 15. Epub 2019 Apr 15.

Nanoscience & Nanotechnology & Innovative Instrumentation (NAST) Centre, University of Rome Tor Vergata, Rome, Italy; Department of Experimental Medicine, University of Rome Tor Vergata, Rome, Italy; Sanford Burnham Prebys Medical Discovery Institute, La Jolla, CA, 92037, USA. Electronic address:

Matrix vesicles (MVs) are a class of extracellular vesicles that initiate mineralization in cartilage, bone, and other vertebrate tissues by accumulating calcium ions (Ca) and inorganic phosphate (P) within their lumen and forming a nucleation core (NC). After further sequestration of Ca and P, the NC transforms into crystalline complexes. Direct evidence of the existence of the NC and its maturation have been provided solely by analyses of dried samples. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S00039861183104
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http://dx.doi.org/10.1016/j.abb.2019.04.003DOI Listing
April 2019
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Golden Palladium Zinc Ordered Intermetallics as Oxygen Reduction Electrocatalysts.

ACS Nano 2019 Apr 18. Epub 2019 Apr 18.

Exploring Pt-free electrocatalysts with high activity and long durability for the oxygen reduction reaction (ORR) has been long pursued by the renewable energy material community. In this work, we have designed an ordered intermetallics PdZn/C (O-PdZn) with a several atomic-layer Pd shell, which achieved a three-fold enhancement in ORR mass activities (MA) in alkaline media, relative to Pd/C and Pt/C. Further Au incorporation in O-PdZn/C (Au-O-PdZn/C) yielded a catalyst with superior durability with less than 10% loss in MA after 30,000 potential cycles. Read More

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http://dx.doi.org/10.1021/acsnano.9b01961DOI Listing

A mouse model of cochlear implantation with chronic electric stimulation.

PLoS One 2019 18;14(4):e0215407. Epub 2019 Apr 18.

Department of Otolaryngology-Head and Neck Surgery, University of Iowa, Iowa City, IA, United States of America.

Objectives: Cochlear implants provide an effective treatment option for those with severe hearing loss, including those with preserved low frequency hearing. However, certain issues can reduce implant efficacy including intracochlear tissue response and delayed loss of residual acoustic hearing. We describe a mouse model of cochlear implantation with chronic electric stimulation that can be used to study cochlear implant biology and related pathologies. Read More

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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0215407PLOS

Ribosomal RNA gene repeats associate with the nuclear pore complex for maintenance after DNA damage.

PLoS Genet 2019 Apr 18;15(4):e1008103. Epub 2019 Apr 18.

Laboratory of Genome Regeneration, Institute for Quantitative Biosciences (IQB), Bunkyo-ku, Japan.

The ribosomal RNA genes (rDNA) comprise a highly repetitive gene cluster. The copy number of genes at this locus can readily change and is therefore one of the most unstable regions of the genome. DNA damage in rDNA occurs after binding of the replication fork blocking protein Fob1 in S phase, which triggers unequal sister chromatid recombination. Read More

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http://dx.doi.org/10.1371/journal.pgen.1008103DOI Listing

A stress-response-related inter-compartmental signalling pathway regulates embryonic cuticle integrity in Arabidopsis.

PLoS Genet 2019 Apr 18;15(4):e1007847. Epub 2019 Apr 18.

Laboratoire Reproduction et Développement des Plantes, Univ Lyon, ENS de Lyon, CNRS, INRA, Lyon, France.

The embryonic cuticle is necessary for normal seed development and seedling establishment in Arabidopsis. Although mutants with defective embryonic cuticles have been identified, neither the deposition of cuticle material, nor its regulation, has been described during embryogenesis. Here we use electron microscopy, cuticle staining and permeability assays to show that cuticle deposition initiates de novo in patches on globular embryos. Read More

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http://dx.doi.org/10.1371/journal.pgen.1007847DOI Listing

Prospective Respiration-Gated Photoacoustic Microscopy.

IEEE Trans Biomed Eng 2019 Apr 16. Epub 2019 Apr 16.

Objective: Photoacoustic microscopy (PAM) is a promising biomedical imaging technique that relies on sequential excitation to generate three-dimensional images. It combines the high contrast of optical imaging with high penetration depth of ultrasound imaging. The normal respiration rate of mice is greater than 3 Hz, which leads to motion artifacts in most reported PAM for in-vivo imaging. Read More

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https://ieeexplore.ieee.org/document/8692629/
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http://dx.doi.org/10.1109/TBME.2019.2911551DOI Listing
April 2019
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Coexistence of Elastic Modulations in the Charge Density Wave State of 2H-NbSe2.

Nano Lett 2019 Apr 18. Epub 2019 Apr 18.

Bulk and single-layer NbSe2 exhibit identical charge density wave order (CDW) with a quasi-commensurate 3x3 superlattice periodicity. Here we combine scanning tunnelling microscopy (STM) imaging at T = 1 K of 2H-NbSe2 with first-principles density functional theory (DFT) calculations to investigate the structural atomic rearrangement of this CDW phase. Our calculations reveal that six different atomic structures are compatible with the 3×3 CDW distortion, although all of them lie on a very narrow energy range of at most 3 meV per formula unit, suggesting the coexistence of such structures. Read More

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http://pubs.acs.org/doi/10.1021/acs.nanolett.9b00268
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http://dx.doi.org/10.1021/acs.nanolett.9b00268DOI Listing
April 2019
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[Effects of Oridonin on Proliferation Apoptosis of Human Multiple Myeloma Cells H929 in Vitro].

Zhongguo Shi Yan Xue Ye Xue Za Zhi 2019 Apr;27(2):458-463

Department of Hematology, Affiliated Hospital, Nanjing University of Traditional Chinese Medicine, Nanjing 210029, Jiangsu Province, China,E-mail:

Objective: To investigate the effects of oridonin (ORI) on the proliferation and apoptosis of human multiple myeloma cell line H929 and its possible mechanism.

Methods: H929 cells were exposed to ORI 0、4、8、12、16、20、24、28、32 μmol/L for 12, 24 and 36 hours respectively. The prolifcration inhibitory effect of ORI on H929 cells was determined by MTT assay and then the working concentrations of ORI were determined. Read More

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http://dx.doi.org/10.19746/j.cnki.issn.1009-2137.2019.02.024DOI Listing
April 2019
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[SIRT2-Reverses Drug-Resistance of HL-60/A through Autophagy Mechanism].

Zhongguo Shi Yan Xue Ye Xue Za Zhi 2019 Apr;27(2):409-414

Department of Third Internal Medicine, Neijiang Traditional Chinese Medicine Hospital, Neijiang 641000, Sichuan Province, China.

Objective: To investigate the effect of targeting the silent information regulator 2 hemolog 2 (SIRT2) expression on the apoptosis of drug-resistant AML cell line HL-60/A and its mechanism.

Methods: The expression of SIRT2 and antophagy-related protein LCT, P62 in HL-60/A and HL-60 was detected by Western blot, the effect of cytorabine on the apoptosis of HL-60/A cells was detected by using Annexin V/PI double staining after targeting inhibition of SIRT2 expression resulting from transfecting HL-60/A cells with SiRNA. The Western blot and transmission electray microscopy were used to detect the cell autophagy. Read More

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http://dx.doi.org/10.19746/j.cnki.issn.1009-2137.2019.02.016DOI Listing
April 2019
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[Effects of a Traditinal Chinese Medicine Compound Regimen on Differentiation and Proliferation of Acute Myeloid Leukemia Cell Lines].

Zhongguo Shi Yan Xue Ye Xue Za Zhi 2019 Apr;27(2):403-408

Laboratory for Stem Cell and Regenerative Medicine,Affiliated Hospital of Weifang Medical College, Weifang 261042, Shandong Province,

Objective: To study the effect of traditional chinese medicine (TCM) compound on myeloid leukemia cells and to explore its anti-leukemic mechanism.

Methods: Myeloid leukemia cell lines were cultured in vitro and treated with TCM compound. The proliferation of the leukemia cells was measured by CCK8 method. Read More

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http://dx.doi.org/10.19746/j.cnki.issn.1009-2137.2019.02.015DOI Listing

[Role of Vascular Niche of Bone Marrow in the Development of MLL-AF9 Induced Acute Myclaid Leukemia].

Zhongguo Shi Yan Xue Ye Xue Za Zhi 2019 Apr;27(2):318-326

State Key Laboratory of Experimental Hematology, Institute of Hematology & Blood Disease Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Tianjin 300020, China.E-mail:

Objective: To investigate the role of bone marrow vascular niche in the development of MLL-AF9 acute myeloid leukemia (AML).

Methods: Transplantation experiments were performed to establish non-radiated MLL-AF9 AML model; the half-bone immunofluorescence staining and tile-scan imaging of two-photon confocal microscopy were used to obtain the data of 3 main bone marrow niche cells; flow cytometry analysis was performed to characterize leukemia cells in different anatomical sites.

Results: In the early stage of MLL-AF9 AML, the proportion of leukemia cells in the metaphysis of the femur was significantly higher than that in the diaphysis. Read More

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http://dx.doi.org/10.19746/j.cnki.issn.1009-2137.2019.02.002DOI Listing
April 2019
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Vapor Phase Growth of Semiconductor Nanowires: Key Developments and Open Questions.

Chem Rev 2019 Apr 18. Epub 2019 Apr 18.

Laboratory of Semiconductor Materials, Institute of Materials , École Polytechnique Fédérale de Lausanne , 1015 Lausanne , Switzerland.

Nanowires are filamentary crystals with a tailored diameter that can be obtained using a plethora of different synthesis techniques. In this review, we focus on the vapor phase, highlighting the most influential achievements along with a historical perspective. Starting with the discovery of VLS, we feature the variety of structures and materials that can be synthesized in the nanowire form. Read More

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http://dx.doi.org/10.1021/acs.chemrev.8b00649DOI Listing

Tracing molybdenum attenuation in mining environments using molybdenum stable isotopes.

Environ Sci Technol 2019 Apr 18. Epub 2019 Apr 18.

Molybdenum contamination is a concern in mining regions worldwide. Better understanding of processes controlling Mo mobility in mine wastes is critical for assessing potential impacts and developing water-quality management strategies associated to this element. Here, we used Mo stable isotope (δMo) analyses to investigate geochemical controls on Mo mobility within a tailings management facility (TMF) featuring oxic and anoxic environments. Read More

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http://dx.doi.org/10.1021/acs.est.9b00766DOI Listing

Photosensitive fluorophores for single-molecule localization microscopy.

ACS Chem Biol 2019 Apr 17. Epub 2019 Apr 17.

The development of single-molecule localization microscopy (SMLM) has sparked a revolution in biological imaging, allowing 'super-resolution' fluorescence microscopy below the diffraction limit of light. The last decade has seen an explosion in not only optical hardware for SMLM but also the development or repurposing of fluorescent proteins and small-molecule fluorescent probes for this technique. In this review, written by chemists for chemists, we detail the history of single-molecule localization microscopy and collate the collection of probes with demonstrated utility in SMLM. Read More

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http://pubs.acs.org/doi/10.1021/acschembio.9b00197
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http://dx.doi.org/10.1021/acschembio.9b00197DOI Listing
April 2019
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Is there any difference between endometrial hyperplasia and endometrial carcinoma in terms of expression of TRPM2 and TRPM7 ion channels?

Turk J Med Sci 2019 Apr 18;49(2):653-660. Epub 2019 Apr 18.

Background/aim: This study compared TRPM2 and TRPM7 ion channel gene expression and immunohistochemical staining in endometrial hyperplasia and endometrium adenocarcinoma.

Materials And Methods: Sections were taken from paraffin blocks of 120 patients who were divided into 6 groups as follows: G1 (n = 20), proliferative endometrium (PE); G2 (n = 20), EH without atypia; G3 (n = 20), EH with atypia; G4 (n = 20), stage 1A, grade 1 EC; G5 (n = 20), stage 1A, grade 2 EC; and G6 (n = 20), stage 1A, grade 3 EC. TRPM2 and TRPM7 genes were analyzed with qRT-PCR in paraffin-embedded tissue samples. Read More

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http://dx.doi.org/10.3906/sag-1810-176DOI Listing

Toxicology in the Super-Resolution Era.

Authors:
Richard Cole

Curr Protoc Toxicol 2019 Apr 18:e77. Epub 2019 Apr 18.

Wadsworth Center, New York State Department of Health, Albany, New York.

Light microscopy has played a central role in science for the past couple of hundred years and will continue to do so. Multiple super-resolution microscopy techniques have been in the headlines for smashing what for more than 100+ years was believed to be the limits of optical microscopy. This resolution improvement enables the visualization of molecular structures and processes on the nano scale. Read More

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http://dx.doi.org/10.1002/cptx.77DOI Listing

Anthecotulide Sesquiterpene Lactone Exhibits Selective Anticancer Effects in Human Malignant Melanoma Cells by Activating Apoptotic and Autophagic Pathways, S-Phase Cell Cycle Arrest, Caspase Activation, and Inhibition of NF-κB Signalling Pathway.

Med Sci Monit 2019 Apr 18;25:2852-2858. Epub 2019 Apr 18.

Department of Dermatology, Shengjing Hospital of China Medical University, Shenyang, Liaoning, China (mainland).

BACKGROUND We examined the anticancer potential of anthecotulide against SK-MEL-24 malignant melanoma cells. The apoptotic and autophagic effects of anthecotulide were also investigated. MATERIAL AND METHODS The cell viability of SK-MEL-24 human malignant melanoma cells was evaluated by WST-1 assay. Read More

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http://dx.doi.org/10.12659/MSM.913771DOI Listing

Enteric nervous system in the European beaver (Castor fiber) pylorus - an immunohistochemical study.

Pol J Vet Sci 2019 Mar;22(1):101-107

Department of Animal Anatomy, Faculty of Veterinary Medicine, University of Warmia and Mazury, Oczapowskiego 13, 10-718 Olsztyn, Poland.

European beaver (Castor fiber), the largest rodent species inhabiting a wide area of Eurasia, feeds mainly on dry parts of plants, bark or wood. Such kind of nourishment needs to be properly digested in each part of the gastrointestinal tract. The time of stomach digestion, which directly influences all the following steps of the digestion process, is precisely controlled by the pylorus and its innervation. Read More

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http://journals.pan.pl/dlibra/publication/127076
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http://dx.doi.org/10.24425/pjvs.2019.127076DOI Listing
March 2019
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Canine post-thaw sperm quality can be predicted by using CASA, and classification and regression tree (CART)-analysis.

Pol J Vet Sci 2019 Mar;22(1):51-59

Institute for Bioinformatics and Biostatistics Vetmeduni Vienna, Veterinärplatz 1, 1210-Vienna, Austria.

In individual dogs, despite good quality of raw sperm, some parameters are significantly changed after thawing, which cannot be predicted. We therefore investigated whether motility parameters objectively obtained by CASA, membrane integrity (MI), cell morphology or a combination are suitable to improve the prediction of bad post-thaw quality. For this purpose 250 sperm analysis protocols from 141 healthy stud dogs, all patients introduced for sperm cryopreservation, were evaluated and a Classification and Regression Tree (CART) -analysis performed. Read More

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http://dx.doi.org/10.24425/pjvs.2018.125607DOI Listing

Phase-Controlled Synthesis of Monolayer Ternary Telluride with a Random Local Displacement of Tellurium Atoms.

Adv Mater 2019 Apr 17:e1900862. Epub 2019 Apr 17.

School of Materials Science and Engineering, Nanyang Technological University, Singapore, 639798, Singapore.

Alloying 2D transition metal dichalcogenides has opened up new opportunities for bandgap engineering and phase control. Developing a simple and scalable synthetic route is therefore essential to explore the full potential of these alloys with tunable optical and electrical properties. Here, the direct synthesis of monolayer WTe S alloys via one-step chemical vapor deposition (CVD) is demonstrated. Read More

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http://dx.doi.org/10.1002/adma.201900862DOI Listing

The endocytic pathways of carbon dots in human adenoid cystic carcinoma cells.

Cell Prolif 2019 Apr 17:e12586. Epub 2019 Apr 17.

Key Laboratory of Shaanxi Province for Craniofacial Precision Medicine Research, College of Stomatology, Xi'an Jiaotong University, Xi'an, China.

Objectives: This study aimed at investigating cellular uptake pathways of carbon dots (CDs) in human adenoid cystic carcinoma cell line ACC-2.

Materials And Methods: We synthesized CDs using a hydrothermal method with citric acid and polyethylenimine (PEI, Mw = 25 000). The CDs incubated with the ACC-2 cells showed their bioimaging capabilities using a confocal microscopy test. Read More

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https://onlinelibrary.wiley.com/doi/abs/10.1111/cpr.12586
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http://dx.doi.org/10.1111/cpr.12586DOI Listing
April 2019
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Evidence of Spin Frustration in a Vanadium Diselenide Monolayer Magnet.

Adv Mater 2019 Apr 18:e1901185. Epub 2019 Apr 18.

Centre for Advanced 2D Materials (CA2DM) and Graphene Research Centre (GRC), National University of Singapore, 6 Science Drive 2, Singapore, 117546, Singapore.

Monolayer VSe , featuring both charge density wave and magnetism phenomena, represents a unique van der Waals magnet in the family of metallic 2D transition-metal dichalcogenides (2D-TMDs). Herein, by means of in situ microscopy and spectroscopic techniques, including scanning tunneling microscopy/spectroscopy, synchrotron X-ray and angle-resolved photoemission, and X-ray absorption, direct spectroscopic signatures are established, that identify the metallic 1T-phase and vanadium 3d electronic configuration in monolayer VSe grown on graphite by molecular-beam epitaxy. Element-specific X-ray magnetic circular dichroism, complemented with magnetic susceptibility measurements, further reveals monolayer VSe as a frustrated magnet, with its spins exhibiting subtle correlations, albeit in the absence of a long-range magnetic order down to 2 K and up to a 7 T magnetic field. Read More

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https://onlinelibrary.wiley.com/doi/abs/10.1002/adma.2019011
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http://dx.doi.org/10.1002/adma.201901185DOI Listing
April 2019
1 Read
17.493 Impact Factor

Key dermoscopic signs in the diagnosis and progression of extrafacial lentigo maligna: Evaluation of a series of 41 cases.

Australas J Dermatol 2019 Apr 17. Epub 2019 Apr 17.

Dermatology Service, Hospital Fundación de Alcorcón, Madrid, Spain.

Background/objectives: Lentigo maligna is usually located on the face. Extrafacial lentigo maligna is less common, and diagnosis of early forms is very difficult. Confocal microscopy of facial and extrafacial lentigo maligna shares the same features (abundant dendritic cells and generalised atypical junctional thickenings) and helps us to identify the dermoscopic features of extrafacial lentigo maligna. Read More

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http://dx.doi.org/10.1111/ajd.13051DOI Listing

Sphingosine Kinase-1 Is Essential for Maintaining External/Outer Limiting Membrane and Associated Adherens Junctions in the Aging Retina.

Mol Neurobiol 2019 Apr 17. Epub 2019 Apr 17.

Department of Cell Biology, University of Oklahoma Health Sciences Center, Oklahoma City, OK, 73104, USA.

Sphingosine-1-phosphate (S1P) produced by sphingosine kinases (SPHK1 and SPHK2) is a signaling molecule involved in cell proliferation and formation of cellular junctions. In this study, we characterized the retinas of Sphk1 knockout (KO) mice by electron microscopy and immunocytochemistry. We also tested cultured Müller glia for their response to S1P. Read More

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http://dx.doi.org/10.1007/s12035-019-1599-xDOI Listing

Fabrication of polydimethylsiloxane nanofluidic chips under AFM tip-based nanomilling process.

Nanoscale Res Lett 2019 Apr 17;14(1):136. Epub 2019 Apr 17.

Center for Precision Engineering, Harbin Institute of Technology, Harbin, 150001, Heilongjiang, People's Republic of China.

In current research realm, polydimethylsiloxane (PDMS)-based nanofluidic devices are widely used in medical, chemical, and biological applications. In the present paper, a novel nanomilling technique (consisting of an AFM system and a piezoelectric actuator) was proposed to fabricate nanochannels (with controllable sizes) on PDMS chips, and nanochannel size was controlled by the driving voltage and frequency inputted to the piezoelectric actuator. Moreover, microchannel and nanochannel molds were respectively fabricated by UV lithography and AFM tip-based nanomilling, and finally, PDMS slabs with micro/nanochannels were obtained by transfer process. Read More

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http://dx.doi.org/10.1186/s11671-019-2962-6DOI Listing

Chymase uptake by cardiomyocytes results in myosin degradation in cardiac volume overload.

Heliyon 2019 Apr 4;5(4):e01397. Epub 2019 Apr 4.

Birmingham Veteran Affairs Medical Center, USA.

Background: Volume overload (VO) of isolated mitral regurgitation (MR) or aortocaval fistula (ACF) is associated with extracellular matrix degradation and cardiomyocyte myofibrillar and desmin breakdown. Left ventricular (LV) chymase activity is increased in VO and recent studies demonstrate chymase presence within cardiomyocytes. Here we test the hypothesis that chymase within the cardiomyocyte coincides with myosin and desmin breakdown in VO. Read More

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http://dx.doi.org/10.1016/j.heliyon.2019.e01397DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6451194PMC

Data from functionalization of diatom mesoporous biosilica with bisphosphonates.

Data Brief 2019 Jun 16;24:103831. Epub 2019 Mar 16.

CNR- ICCOM, Department of Chemistry, University of Bari "Aldo Moro", Via E. Orabona, 4, 70126 Bari, Italy.

Diatoms are unicellular photosynthetic microalgae that produce a sophisticated mesoporous biosilica shell called . Easy to achieve and extract, diatom frustules represent a low-cost source of mesoporous biocompatible biosilica. In this paper, the possibility to functionalize the diatom biosilica with bisphosphonates (BPs) was investigated. Read More

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http://dx.doi.org/10.1016/j.dib.2019.103831DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6451687PMC

Electrocatalytic water oxidation over AlFeB.

Chem Sci 2019 Mar 23;10(9):2796-2804. Epub 2019 Jan 23.

Department of Chemistry and Biochemistry , Florida State University , 95 Chieftan Way , Tallahassee , FL 32306 , USA . Email:

We report excellent electrocatalytic performance by AlFeB in the oxygen-evolution reaction (OER). The inexpensive catalytic material, prepared simply by arc-melting followed by ball-milling, exhibits high stability and sustained catalytic performance under alkaline conditions. The overpotential value of 0. Read More

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http://xlink.rsc.org/?DOI=C8SC04106G
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http://dx.doi.org/10.1039/c8sc04106gDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6425857PMC
March 2019
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Faceted polymersomes: a sphere-to-polyhedron shape transformation.

Chem Sci 2019 Mar 10;10(9):2725-2731. Epub 2019 Jan 10.

School of Chemistry , University of New South Wales , NSW 2052 , Australia . Email: ; Email:

The creation of "soft" deformable hollow polymeric nanoparticles with complex non-spherical shapes block copolymer self-assembly remains a challenge. In this work, we show that a perylene-bearing block copolymer can self-assemble into polymeric membrane sacs (polymersomes) that not only possess uncommonly faceted polyhedral shapes but are also intrinsically fluorescent. Here, we further reveal for the first time an experimental visualization of the entire polymersome faceting process. Read More

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http://xlink.rsc.org/?DOI=C8SC04206C
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http://dx.doi.org/10.1039/c8sc04206cDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6419931PMC
March 2019
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Highly reversible potassium-ion intercalation in tungsten disulfide.

Chem Sci 2019 Mar 27;10(9):2604-2612. Epub 2018 Dec 27.

CAS Key Laboratory of Design and Assembly of Functional Nanostructures, and Fujian Key Laboratory of Nanomaterials , Fujian Institute of Research on the Structure of Matter , Chinese Academy of Sciences , Fuzhou , Fujian 350002 , P.R. China . Email:

Rechargeable potassium-ion batteries (PIBs) show promise beyond Li-ion technology in large-scale electrical-energy storage due to the abundance and low cost of potassium resources. However, the intercalation of large-size K generally results in irreversible structural degradation and short lifespan to the hosts, representing a major obstacle. Here, we report a new electrochemical K-intercalation host, tungsten disulfide (WS), which can store 0. Read More

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http://xlink.rsc.org/?DOI=C8SC04350G
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http://dx.doi.org/10.1039/c8sc04350gDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6419940PMC
March 2019
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Single-site metal-organic framework catalysts for the oxidative coupling of arenes C-H/C-H activation.

Chem Sci 2019 Mar 18;10(12):3616-3622. Epub 2019 Feb 18.

Centre for Surface Chemistry and Catalysis , KU Leuven , Celestijnenlaan 200F P. O. Box 2461 , 3001 Leuven , Belgium . Email:

C-H activation reactions are generally associated with relatively low turnover numbers (TONs) and high catalyst concentrations due to a combination of low catalyst stability and activity, highlighting the need for recyclable heterogeneous catalysts with stable single-atom active sites. In this work, several palladium loaded metal-organic frameworks (MOFs) were tested as single-site catalysts for the oxidative coupling of arenes (-xylene) C-H/C-H activation. Isolation of the palladium active sites on the MOF supports reduced Pd(0) aggregate formation and thus catalyst deactivation, resulting in higher turnover numbers (TONs) compared to the homogeneous benchmark reaction. Read More

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http://xlink.rsc.org/?DOI=C8SC05510F
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http://dx.doi.org/10.1039/c8sc05510fDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6432273PMC
March 2019
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Flux melting of metal-organic frameworks.

Chem Sci 2019 Mar 12;10(12):3592-3601. Epub 2019 Feb 12.

Department of Materials Science and Metallurgy , University of Cambridge , Charles Babbage Road , Cambridge , CB3 0FS , UK . Email:

Recent demonstrations of melting in the metal-organic framework (MOF) family have created interest in the interfacial domain between inorganic glasses and amorphous organic polymers. The chemical and physical behaviour of porous hybrid liquids and glasses is of particular interest, though opportunities are limited by the inaccessible melting temperatures of many MOFs. Here, we show that the processing technique of flux melting, 'borrowed' from the inorganic domain, may be applied in order to melt ZIF-8, a material which does not possess an accessible liquid state in the pure form. Read More

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http://xlink.rsc.org/?DOI=C8SC04044C
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http://dx.doi.org/10.1039/c8sc04044cDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6430010PMC
March 2019
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Selective high-resolution DNP-enhanced NMR of biomolecular binding sites.

Chem Sci 2019 Mar 1;10(11):3366-3374. Epub 2019 Feb 1.

Univ. Grenoble Alpes , CEA , CNRS , INAC-MEM , Grenoble , France . Email: ; Email:

Locating binding sites in biomolecular assemblies and solving their structures are of the utmost importance to unravel functional aspects of the system and provide experimental data that can be used for structure-based drug design. This often still remains a challenge, both in terms of selectivity and sensitivity for X-ray crystallography, cryo-electron microscopy and NMR. In this work, we introduce a novel method called Selective Dynamic Nuclear Polarization (Sel-DNP) that allows selective highlighting and identification of residues present in the binding site. Read More

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http://xlink.rsc.org/?DOI=C8SC05696J
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http://dx.doi.org/10.1039/c8sc05696jDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6429603PMC
March 2019
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Downsizing feature of microphase-separated structures intramolecular crosslinking of block copolymers.

Chem Sci 2019 Mar 14;10(11):3330-3339. Epub 2019 Jan 14.

Faculty of Engineering and Graduate School of Chemical Sciences and Engineering , Hokkaido University , Sapporo 060-8628 , Japan . Email: ; Email:

A novel strategy for downsizing the feature of microphase-separated structures was developed the intramolecular crosslinking reaction of block copolymers (BCPs) without changing the molecular weight. A series of BCPs consisting of poly[styrene--(-3-butenyl styrene)] and poly(-lactide) (SBS-LA) was subjected to Ru-catalyzed olefin metathesis under highly diluted conditions to produce intramolecularly crosslinked BCPs (SBS()-LAs). Small-angle X-ray scattering measurement and transmission electron microscopy observation of the SBS()-LAs revealed feature size reduction in lamellar (LAM) and hexagonally close-packed cylinder (HEX) structures in the bulk state, which was surely due to the restricted chain dimensions of the intramolecularly crosslinked SBS block. Read More

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http://xlink.rsc.org/?DOI=C8SC05016C
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http://dx.doi.org/10.1039/c8sc05016cDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6429781PMC
March 2019
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Addressing the properties of "Metallo-DNA" with a Ag(i)-mediated supramolecular duplex.

Chem Sci 2019 Mar 8;10(11):3186-3195. Epub 2019 Feb 8.

Chemical Nanoscience Laboratory , School of Natural & Environmental Sciences , Newcastle University , Newcastle upon Tyne , NE1 7RU , UK . Email:

The silver-nucleoside complex [Ag(i)-(3-cytidine)], , self-assembles to form a supramolecular metal-mediated base-pair array highly analogous to those seen in metallo-DNA. A combination of complementary hydrogen-bonding, hydrophobic and argentophilic interactions drive the formation of a double-helix with a continuous silver core. Electrical measurements on show that despite having Ag···Ag distances within <5% of the metallic radii, the material is electrically insulating. Read More

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http://xlink.rsc.org/?DOI=C8SC05103H
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http://dx.doi.org/10.1039/c8sc05103hDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6429620PMC
March 2019
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Blood supply in the bicipital groove: A histological analysis.

Orthop Rev (Pavia) 2019 Feb 29;11(1):8106. Epub 2019 Mar 29.

Clinic for Orthopedics.

The vascular anatomy in the closed bicipital groove with the long head of the biceps brachii muscle tendon (LHBT), its mesotenon and the transverse ligament intact has not been analyzed on a histological level yet. An anatomic dissection and histologic study was conducted by using 24 cadaveric formaldehyde fixated shoulders. The bicipital groove including the LHBT and its intact sheath was cut , fixated, sliced in 7 μm sections, Azan stained and the vascular anatomy analyzed under light microscopy. Read More

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http://dx.doi.org/10.4081/or.2019.8106DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6452089PMC
February 2019

Identification of Isoform 2 Acid-Sensing Ion Channel Inhibitors as Tool Compounds for Target Validation Studies in CNS.

ACS Med Chem Lett 2019 Apr 7;10(4):627-632. Epub 2019 Feb 7.

Promidis, Via Olgettina 60, 20132 Milan, Italy.

Acid-sensing ion channels (ASICs) are a family of ion channels permeable to cations and largely responsible for the onset of acid-evoked ion currents both in neurons and in different types of cancer cells, thus representing a potential target for drug discovery. Owing to the limited attention ASIC2 has received so far, an exploratory program was initiated to identify ASIC2 inhibitors using diminazene, a known -ASIC inhibitor, as a chemical starting point for structural elaboration. The performed exploration enabled the identification of a novel series of ASIC2 inhibitors. Read More

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http://pubs.acs.org/doi/10.1021/acsmedchemlett.8b00591
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http://dx.doi.org/10.1021/acsmedchemlett.8b00591DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6466820PMC
April 2019
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Self-assembling Releasable Thiocolchicine-Diphenylbutenylaniline Conjugates.

ACS Med Chem Lett 2019 Apr 4;10(4):611-614. Epub 2019 Jan 4.

Dipartimento di Chimica, Università degli Studi di Milano, Via Golgi 19, 20133 Milano, Italy.

The design and the synthesis of new self-assembling conjugates is reported. The target compounds are characterized by the presence of a self-immolative linker that secures a controlled release induced by lipase cleavage. 4-(1,2-Diphenylbut-1-en-1-yl)aniline is used as a self-assembling inducer and amino-thiocolchicine as prototype of drug. Read More

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http://pubs.acs.org/doi/10.1021/acsmedchemlett.8b00605
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http://dx.doi.org/10.1021/acsmedchemlett.8b00605DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6466830PMC
April 2019
1 Read

Multifunctional Carrier Based on Halloysite/Laponite Hybrid Hydrogel for Kartogenin Delivery.

ACS Med Chem Lett 2019 Apr 22;10(4):419-424. Epub 2018 Oct 22.

Dipartimento STEBICEF, Sez. Chimica, Università degli Studi di Palermo, Viale delle Scienze, Ed. 17, 90128 Palermo, Italy.

A novel carrier system based on halloysite nanotubes (HNT), for the potential intraarticular delivery of kartogenin (KGN) by means laponite (Lap) hydrogel (HNT/KGN/Lap), is developed. The drug was first loaded into HNT, and the hybrid composite obtained was used as filler for laponite hydrogel. Both the filler and the hydrogel were thoroughly investigated by several techniques and the hydrogel morphology was imaged by transmission electron microscopy. Read More

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http://dx.doi.org/10.1021/acsmedchemlett.8b00465DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6466553PMC
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