1,902 results match your criteria Microbial Cell Factories [Journal]


Development of a secretory expression system with high compatibility between expression elements and an optimized host for endoxylanase production in Corynebacterium glutamicum.

Microb Cell Fact 2019 Apr 17;18(1):72. Epub 2019 Apr 17.

National Engineering Laboratory for Cereal Fermentation Technology, Jiangnan University, Wuxi, 214122, China.

Background: In terms of protein production, the internal environment of the host influences the activity of expression elements, thus affecting the expression level of the target protein. Native expression elements from a specific strain always function well in the original host. In the present study, to enhance the endoxylanase (XynA) production level in Corynebacterium glutamicum CGMCC1. Read More

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http://dx.doi.org/10.1186/s12934-019-1116-yDOI Listing

Tailoring Corynebacterium glutamicum towards increased malonyl-CoA availability for efficient synthesis of the plant pentaketide noreugenin.

Microb Cell Fact 2019 Apr 11;18(1):71. Epub 2019 Apr 11.

Institute of Bio- and Geosciences, IBG-1: Biotechnology, Forschungszentrum Jülich, 52425, Jülich, Germany.

Background: In the last years, different biotechnologically relevant microorganisms have been engineered for the synthesis of plant polyphenols such as flavonoids and stilbenes. However, low intracellular availability of malonyl-CoA as essential precursor for most plant polyphenols of interest is regarded as the decisive bottleneck preventing high product titers.

Results: In this study, Corynebacterium glutamicum, which emerged as promising cell factory for plant polyphenol production, was tailored by rational metabolic engineering towards providing significantly more malonyl-CoA for product synthesis. Read More

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http://dx.doi.org/10.1186/s12934-019-1117-xDOI Listing

Construction of a bacterial surface display system based on outer membrane protein F.

Microb Cell Fact 2019 Apr 11;18(1):70. Epub 2019 Apr 11.

State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, School of Life Sciences, Xiamen University, Xiamen, 361102, China.

Background: Bacterial surface display systems were developed to surface expose heterologous proteins or peptides for different applications, such as peptide libraries screening and live bacterial vaccine design. Various outer membrane proteins, such as outer membrane protein A (OmpA), OmpC and outer membrane pore protein E precursor (PhoE), have been used as carriers for surface display, fused to the proteins or peptides of interest in Gram-negative bacteria. Here, we investigated the utility of constitutively expressed OmpF for the display of foreign immune epitopes on the Escherichia coli cell surface and then compared it with plasmid-induced expression of OmpF and OmpC. Read More

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https://microbialcellfactories.biomedcentral.com/articles/10
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http://dx.doi.org/10.1186/s12934-019-1120-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6458713PMC
April 2019
3 Reads

Enhanced extracellular expression of Bacillus stearothermophilus α-amylase in Bacillus subtilis through signal peptide optimization, chaperone overexpression and α-amylase mutant selection.

Microb Cell Fact 2019 Apr 11;18(1):69. Epub 2019 Apr 11.

State Key Laboratory of Food Science and Technology, Jiangnan University, 1800 Lihu Avenue, Wuxi, 214122, Jiangsu, China.

Background: Our laboratory has constructed a Bacillus stearothermophilus α-amylase (AmyS) derivative with excellent enzymatic properties. Bacillus subtilis is generally regarded as safe and has excellent protein secretory capability, but heterologous extracellular production level of B. stearothermophilus α-amylase in B. Read More

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http://dx.doi.org/10.1186/s12934-019-1119-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6458788PMC

High-yield production of multiple O-methylated phenylpropanoids by the engineered Escherichia coli-Streptomyces cocultivation system.

Microb Cell Fact 2019 Apr 10;18(1):67. Epub 2019 Apr 10.

Department of Chemistry and Nanoscience, Ewha Womans University, Ewhayeodae-gil 52, Seoul, 03760, Republic of Korea.

Background: O-Methylated phenylpropanoids, which are generally present in small amounts in plants, have improved or distinct biological activities and pharmacological properties as opposed to their unmethylated counterparts. Although microbial production could be a useful tool for the efficient and environment-friendly production of methylated phenylpropanoids, a high-yield microbial production of neither tri-methylated stilbenes nor di-/tri-methylated flavonoids has been achieved to date.

Results: A methyltransferase from Streptomyces avermitilis (SaOMT2), which has been known to possess 7-O-methylation activity toward several flavonoids, exhibited more diverse regiospecificity and catalyzed mono-, di-, and tri-methylation of stilbene, flavanone, and flavone when it was expressed in Streptomyces venezuelae. Read More

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http://dx.doi.org/10.1186/s12934-019-1118-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6456975PMC
April 2019
4.221 Impact Factor

Enhanced production of antifungal lipopeptide iturin A by Bacillus amyloliquefaciens LL3 through metabolic engineering and culture conditions optimization.

Microb Cell Fact 2019 Apr 10;18(1):68. Epub 2019 Apr 10.

Key Laboratory of Molecular Microbiology and Technology for Ministry of Education, Nankai University, Tianjin, 300071, China.

Background: Iturins, which belong to antibiotic cyclic lipopeptides mainly produced by Bacillus sp., have the potential for application in biomedicine and biocontrol because of their hemolytic and antifungal properties. Bacillus amyloliquefaciens LL3, isolated previously by our lab, possesses a complete iturin A biosynthetic pathway as shown by genomic analysis. Read More

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http://dx.doi.org/10.1186/s12934-019-1121-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6457013PMC

Spontaneous C-cleavage of a truncated intein as fusion tag to produce tag-free VP1 inclusion body nanoparticle vaccine against CVB3-induced viral myocarditis by the oral route.

Microb Cell Fact 2019 Apr 4;18(1):66. Epub 2019 Apr 4.

The Jiangsu Key Laboratory of Infection and Immunity, Institutes of Biology and Medical Sciences, Soochow University, Suzhou, 215123, Jiangsu, China.

Background: Oral vaccine is highly desired for infectious disease which is caused by pathogens infection through the mucosal surface. The design of suitable vaccine delivery system is ongoing for the antigen protection from the harsh gastric environment and target to the Peyer's patches to induce sufficient mucosal immune responses. Among various potential delivery systems, bacterial inclusion bodies have been widely used as delivery systems in the field of nanobiomedicine. Read More

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http://dx.doi.org/10.1186/s12934-019-1115-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6449988PMC
April 2019
1 Read

Equilibrium of the intracellular redox state for improving cell growth and L-lysine yield of Corynebacterium glutamicum by optimal cofactor swapping.

Microb Cell Fact 2019 Apr 3;18(1):65. Epub 2019 Apr 3.

The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, 1800# Lihu Road, Wuxi, 214122, China.

Background: NAD(H/) and NADP(H/) are the most important redox cofactors in bacteria. However, the intracellular redox balance is in advantage of the cell growth and production of NAD(P)H-dependent products.

Results: In this paper, we rationally engineered glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and isocitrate dehydrogenase (IDH) to switch the nucleotide-cofactor specificity resulting in an increase in final titer [from 85. Read More

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https://microbialcellfactories.biomedcentral.com/articles/10
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http://dx.doi.org/10.1186/s12934-019-1114-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6448238PMC
April 2019
3 Reads

Construction of DGLA producing cell factory by genetic modification of Mucor circinelloides.

Microb Cell Fact 2019 Apr 3;18(1):64. Epub 2019 Apr 3.

Colin Ratledge Center for Microbial Lipids, School of Agricultural Engineering and Food Science, Shandong University of Technology, Zibo, 255000, Shandong, People's Republic of China.

Background: Dihomo-gamma linolenic acid (DGLA, 20:3, n-6) is the elongated product of Gamma linolenic acid (GLA, 18:3, n-6) catalyzed by the enzyme delta-6 elongase (D6E) or gamma linolenic acid elongase (GLELO). Construction of engineered oleaginous microbes have been attracting significant interest to produce DGLA because of its nutritional value and medicinal applications. Mucor circinelloides is a GLA producing filamentous fungus which can be a useful tool to produce DGLA. Read More

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http://dx.doi.org/10.1186/s12934-019-1110-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6448318PMC

CRISPR-mediated genome editing in non-conventional yeasts for biotechnological applications.

Microb Cell Fact 2019 Apr 2;18(1):63. Epub 2019 Apr 2.

Division of Biotechnology, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, 457 Zhongshan Road, Dalian, 116023, People's Republic of China.

Non-conventional yeasts are playing important roles as cell factories for bioproduction of biofuels, food additives and proteins with outstanding natural characteristics. However, the precise genome editing is challenging in non-conventional yeasts due to lack of efficient genetic tools. In the past few years, CRISPR-based genome editing worked as a revolutionary tool for genetic engineering and showed great advantages in cellular metabolic engineering. Read More

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http://dx.doi.org/10.1186/s12934-019-1112-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6444819PMC
April 2019
1 Read

Metabolic engineering of Ashbya gossypii for deciphering the de novo biosynthesis of γ-lactones.

Microb Cell Fact 2019 Mar 28;18(1):62. Epub 2019 Mar 28.

CEB-Centre of Biological Engineering, University of Minho, 4710-057, Braga, Portugal.

Background: Lactones are highly valuable cyclic esters of hydroxy fatty acids that find application as pure fragrances or as building blocks of speciality chemicals. While chemical synthesis often leads to undesired racemic mixtures, microbial production allows obtaining optically pure lactones. The production of a specific lactone by biotransformation depends on the supply of the corresponding hydroxy fatty acid, which has economic and industrial value similar to γ-lactones. Read More

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http://dx.doi.org/10.1186/s12934-019-1113-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6437850PMC
March 2019
1 Read

Engineering metabolite-responsive transcriptional factors to sense small molecules in eukaryotes: current state and perspectives.

Microb Cell Fact 2019 Mar 26;18(1):61. Epub 2019 Mar 26.

Department of Chemical Biochemical and Environmental Engineering, University of Maryland Baltimore County, Baltimore, MD, 21250, USA.

Nature has evolved exquisite sensing mechanisms to detect cellular and environmental signals surrounding living organisms. These biosensors have been widely used to sense small molecules, detect environmental cues and diagnose disease markers. Metabolic engineers and synthetic biologists have been able to exploit metabolites-responsive transcriptional factors (MRTFs) as basic tools to rewire cell metabolism, reprogram cellular activity as well as boost cell's productivity. Read More

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http://dx.doi.org/10.1186/s12934-019-1111-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6434827PMC

Redesign of a novel D-allulose 3-epimerase from Staphylococcus aureus for thermostability and efficient biocatalytic production of D-allulose.

Microb Cell Fact 2019 Mar 25;18(1):59. Epub 2019 Mar 25.

Key Laboratory of Industrial Fermentation Microbiology of the Ministry of Education, Tianjin Key Laboratory of Industrial Microbiology, College of Biotechnology, National Engineering Laboratory for Industrial Enzymes, Tianjin University of Science and Technology, Tianjin, 300457, People's Republic of China.

Background: A novel D-allulose 3-epimerase from Staphylococcus aureus (SaDAE) has been screened as a D-allulose 3-epimerase family enzyme based on its high specificity for D-allulose. It usually converts both D-fructose and D-tagatose to respectively D-allulose and D-sorbose. We targeted potential biocatalysts for the large-scale industrial production of rare sugars. Read More

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http://dx.doi.org/10.1186/s12934-019-1107-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6432756PMC
March 2019
2 Reads

Optimizing a CRISPR-Cpf1-based genome engineering system for Corynebacterium glutamicum.

Microb Cell Fact 2019 Mar 25;18(1):60. Epub 2019 Mar 25.

Key Laboratory of Molecular Medicine and Biotherapy, School of Life Sciences, Beijing Institute of Technology, No. 5 South Zhongguancun Street, Beijing, 100081, China.

Background: Corynebacterium glutamicum is an important industrial strain for the production of a diverse range of chemicals. Cpf1 nucleases are highly specific and programmable, with efficiencies comparable to those of Cas9. Although the Francisella novicida (Fn) CRISPR-Cpf1 system has been adapted for genome editing in C. Read More

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http://dx.doi.org/10.1186/s12934-019-1109-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6432761PMC

Metabolic engineering of Escherichia coli for efficient biosynthesis of fluorescent phycobiliprotein.

Microb Cell Fact 2019 Mar 20;18(1):58. Epub 2019 Mar 20.

Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, 266071, China.

Background: Phycobiliproteins (PBPs) are light-harvesting protein found in cyanobacteria, red algae and the cryptomonads. They have been widely used as fluorescent labels in cytometry and immunofluorescence analysis. A number of PBPs has been produced in metabolically engineered Escherichia coli. Read More

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http://dx.doi.org/10.1186/s12934-019-1100-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6425641PMC
March 2019
2 Reads

Precise tuning of the glyoxylate cycle in Escherichia coli for efficient tyrosine production from acetate.

Microb Cell Fact 2019 Mar 19;18(1):57. Epub 2019 Mar 19.

School of Interdisciplinary Bioscience and Bioengineering, Pohang University of Science and Technology, 77 Cheongam-Ro, Nam-Gu, Pohang, Gyeongbuk, 37673, South Korea.

Background: Acetate is one of promising feedstocks owing to its cheap price and great abundance. Considering that tyrosine production is gradually shifting to microbial production method, its production from acetate can be attempted to further improve the economic feasibility of its production.

Results: Here, we engineered a previously reported strain, SCK1, for efficient production of tyrosine from acetate. Read More

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http://dx.doi.org/10.1186/s12934-019-1106-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6423740PMC

Challenges and tackles in metabolic engineering for microbial production of carotenoids.

Microb Cell Fact 2019 Mar 18;18(1):55. Epub 2019 Mar 18.

Division of Applied Life Science (BK21 Plus), PMBBRC, Gyeongsang National University, 501 Jinju-daero, Jinju, 52828, Republic of Korea.

Naturally occurring carotenoids have been isolated and used as colorants, antioxidants, nutrients, etc. in many fields. There is an ever-growing demand for carotenoids production. Read More

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http://dx.doi.org/10.1186/s12934-019-1105-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6421696PMC

Monoterpene production by the carotenogenic yeast Rhodosporidium toruloides.

Microb Cell Fact 2019 Mar 18;18(1):54. Epub 2019 Mar 18.

Biomass Science and Conversion Technology, Sandia National Laboratories, 7011 East Ave, Livermore, CA, 94551, USA.

Background: Due to their high energy density and compatible physical properties, several monoterpenes have been investigated as potential renewable transportation fuels, either as blendstocks with petroleum or as drop-in replacements for use in vehicles (both heavy and light-weight) or in aviation. Sustainable microbial production of these biofuels requires the ability to utilize cheap and readily available feedstocks such as lignocellulosic biomass, which can be depolymerized into fermentable carbon sources such as glucose and xylose. However, common microbial production platforms such as the yeast Saccharomyces cerevisiae are not naturally capable of utilizing xylose, hence requiring extensive strain engineering and optimization to efficiently utilize lignocellulosic feedstocks. Read More

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http://dx.doi.org/10.1186/s12934-019-1099-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6421710PMC
March 2019
1 Read
4.221 Impact Factor

Antibacterial and anticancer activities of orphan biosynthetic gene clusters from Atlantis II Red Sea brine pool.

Microb Cell Fact 2019 Mar 18;18(1):56. Epub 2019 Mar 18.

Graduate Program of Biotechnology, School of Sciences and Engineering, The American University in Cairo, New Cairo, Cairo, 11835, Egypt.

Background: Cancer and infectious diseases are problematic because of continuous emergence of drug resistance. One way to address this enormous global health threat is bioprospecting the unlikeliest environments, such as extreme marine niches, which have tremendous biodiversity that is barely explored. One such environment is the Red Sea brine pool, Atlantis II Deep (ATII). Read More

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http://dx.doi.org/10.1186/s12934-019-1103-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6423787PMC

Increased triacylglycerol production in oleaginous microalga Neochloris oleoabundans by overexpression of plastidial lysophosphatidic acid acyltransferase.

Microb Cell Fact 2019 Mar 13;18(1):53. Epub 2019 Mar 13.

Institute of Molecular Biosciences, Mahidol University, Salaya Campus, Phutthamonthon, Nakhon Pathom, 73170, Thailand.

Background: Microalgae are promising sources of lipid triacylglycerol (TAG) for sustainable production of natural edible oils and biofuels. Nevertheless, products derived from microalgal TAG are not yet economically feasible; increasing TAG content via targeted genetic engineering of genes in TAG biosynthesis pathway are important to achieve economic viability. To increase TAG content, oleaginous microalga Neochloris oleoabundans was genetically engineered with the endogenous enzyme lysophosphatidic acid acyltransferase (NeoLPAAT1) responsible for plastidial TAG biosynthesis RESULTS: NeoLPAAT1 was found to contain all canonical motifs attributed to LPAAT proteins, two hypothetical membrane-spanning domains and a putative chloroplast transit peptide, indicating as a member of plastidial LPAAT type 1 subfamily. Read More

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http://dx.doi.org/10.1186/s12934-019-1104-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6415348PMC
March 2019
1 Read

Synthetic metabolic pathway for the production of 1-alkenes from lignin-derived molecules.

Microb Cell Fact 2019 Mar 11;18(1):48. Epub 2019 Mar 11.

Faculty of Engineering and Natural Sciences, Tampere University, PO Box 527, Tampere, FI-33014, Finland.

Background: Integration of synthetic metabolic pathways to catabolically diverse chassis provides new opportunities for sustainable production. One attractive scenario is the use of abundant waste material to produce a readily collectable product, which can reduce the production costs. Towards that end, we established a cellular platform for the production of semivolatile medium-chain α-olefins from lignin-derived molecules: we constructed 1-undecene synthesis pathway in Acinetobacter baylyi ADP1 using ferulate, a lignin-derived model compound, as the sole carbon source for both cell growth and product synthesis. Read More

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https://microbialcellfactories.biomedcentral.com/articles/10
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http://dx.doi.org/10.1186/s12934-019-1097-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6410514PMC
March 2019
3 Reads
4.221 Impact Factor

Screening and purification of nanobodies from E. coli culture supernatants using the hemolysin secretion system.

Microb Cell Fact 2019 Mar 11;18(1):47. Epub 2019 Mar 11.

Department of Microbial Biotechnology, Centro Nacional de Biotecnología, Consejo Superior de Investigaciones Científicas (CSIC), Campus UAM-Cantoblanco, 28049, Madrid, Spain.

Background: The hemolysin (Hly) secretion system of E. coli allows the one-step translocation of hemolysin A (HlyA) from the bacterial cytoplasm to the extracellular medium, without a periplasmic intermediate. In this work, we investigate whether the Hly secretion system of E. Read More

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http://dx.doi.org/10.1186/s12934-019-1094-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6410518PMC
March 2019
1 Read

Development of a novel, robust and cost-efficient process for valorizing dairy waste exemplified by ethanol production.

Microb Cell Fact 2019 Mar 11;18(1):51. Epub 2019 Mar 11.

National Food Institute, Technical University of Denmark, 2800, Kongens Lyngby, Denmark.

Background: Delactosed whey permeate (DWP) is a side stream of whey processing, which often is discarded as waste, despite of its high residual content of lactose, typically 10-20%. Microbial fermentation is one of the most promising approaches for valorizing nutrient rich industrial waste streams, including those generated by the dairies. Here we present a novel microbial platform specifically designed to generate useful compounds from dairy waste. Read More

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http://dx.doi.org/10.1186/s12934-019-1091-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6410493PMC

Increasing jojoba-like wax ester production in Saccharomyces cerevisiae by enhancing very long-chain, monounsaturated fatty acid synthesis.

Microb Cell Fact 2019 Mar 11;18(1):49. Epub 2019 Mar 11.

Department of Biology and Biological Engineering, Systems and Synthetic Biology, Chalmers University of Technology, Kemivägen 10, 412 96, Gothenburg, Sweden.

Background: Fatty acids (FAs) with a chain length of more than 18 carbon atoms (> C18) are interesting for the production of specialty compounds derived from these FAs. These compounds include free FAs, like erucic acid (C22:1-Δ13), primary fatty alcohols (FOHs), like docosanol (C22:0-FOH), as well as jojoba-like wax esters (WEs) (C38-WE to C44-WE), which are esters of (very) long-chain FAs and (very) long-chain FOHs. In particular, FAs, FOHs and WEs are used in the production of chemicals, pharmaceuticals and cosmetic products. Read More

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http://dx.doi.org/10.1186/s12934-019-1098-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6410506PMC

Metabolic engineering of microbial cell factories for production of nutraceuticals.

Microb Cell Fact 2019 Mar 11;18(1):46. Epub 2019 Mar 11.

Institute for Cellular and Molecular Biology, The University of Texas at Austin, Austin, TX, USA.

Metabolic engineering allows for the rewiring of basic metabolism to overproduce both native and non-native metabolites. Among these biomolecules, nutraceuticals have received considerable interest due to their health-promoting or disease-preventing properties. Likewise, microbial engineering efforts to produce these value-added nutraceuticals overcome traditional limitations of low yield from extractions and complex chemical syntheses. Read More

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http://dx.doi.org/10.1186/s12934-019-1096-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6410520PMC
March 2019
6 Reads

SCRaMbLE generates evolved yeasts with increased alkali tolerance.

Microb Cell Fact 2019 Mar 11;18(1):52. Epub 2019 Mar 11.

Frontier Science Center for Synthetic Biology and Key Laboratory of Systems Bioengineering (Ministry of Education), Tianjin University, Tianjin, 300072, China.

Background: Strains with increased alkali tolerance have a broad application in industrial, especially for bioremediation, biodegradation, biocontrol and production of bio-based chemicals. A novel synthetic chromosome recombination and modification by LoxP-mediated evolution (SCRaMbLE) system has been introduced in the synthetic yeast genome (Sc 2.0), which enables generation of a yeast library with massive structural variations and potentially drives phenotypic evolution. Read More

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http://dx.doi.org/10.1186/s12934-019-1102-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6410612PMC

Exploring small-scale chemostats to scale up microbial processes: 3-hydroxypropionic acid production in S. cerevisiae.

Microb Cell Fact 2019 Mar 11;18(1):50. Epub 2019 Mar 11.

The Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, Kongens Lyngby, Denmark.

Background: The physiological characterization of microorganisms provides valuable information for bioprocess development. Chemostat cultivations are a powerful tool for this purpose, as they allow defined changes to one single parameter at a time, which is most commonly the growth rate. The subsequent establishment of a steady state then permits constant variables enabling the acquisition of reproducible data sets for comparing microbial performance under different conditions. Read More

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http://dx.doi.org/10.1186/s12934-019-1101-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6410522PMC

Pectinase secreted by psychrotolerant fungi: identification, molecular characterization and heterologous expression of a cold-active polygalacturonase from Tetracladium sp.

Microb Cell Fact 2019 Mar 7;18(1):45. Epub 2019 Mar 7.

Laboratorio de Genética, Departamento de Ciencias Ecológicas, Facultad de Ciencias, Universidad de Chile, Las Palmeras 3425, Casilla 653, Santiago, Chile.

Background: Pectinolytic enzymes, which are used in several industries, especially in the clarification process during wine and fruit juice production, represent approximately 10% of the global enzyme market. To prevent the proliferation of undesired microorganisms, to retain labile and volatile flavor compounds, and to save energy, the current trend is to perform this process at low temperatures. However, the commercially available pectinases are highly active at temperatures approximately 50 °C and poorly active at temperatures below 35 °C, which is the reason why there is a constant search for cold-active pectinases. Read More

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http://dx.doi.org/10.1186/s12934-019-1092-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6407229PMC

Optimization of secretion and surface localization of heterologous OVA protein in mycobacteria by using LipY as a carrier.

Microb Cell Fact 2019 Mar 6;18(1):44. Epub 2019 Mar 6.

Medical Microbiology and Infection Control, Cancer Center Amsterdam, Amsterdam UMC, Vrije Universiteit Amsterdam, De Boelelaan 1117, Amsterdam, Netherlands.

Background: Mycobacterium bovis Bacille Calmette-Guérin (BCG) is not only used as a vaccine against tuberculosis but also protects against leprosy and is used as part of bladder cancer treatment to induce a protective immune response. However, protection by BCG vaccination is not optimal. To improve vaccine efficacy, recombinant BCG expressing heterologous antigens has been put forward to elicit antigen-specific cellular and humoral responses. Read More

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http://dx.doi.org/10.1186/s12934-019-1093-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6402100PMC
March 2019
3 Reads

Fermentative production of the unnatural amino acid L-2-aminobutyric acid based on metabolic engineering.

Microb Cell Fact 2019 Feb 28;18(1):43. Epub 2019 Feb 28.

Key Laboratory of Bioorganic Synthesis of Zhejiang Province, College of Biotechnology and Bioengineering, Zhejiang University of Technology, Hangzhou, 310014, China.

Background: L-2-aminobutyric acid (L-ABA) is an unnatural amino acid that is a key intermediate for the synthesis of several important pharmaceuticals. To make the biosynthesis of L-ABA environmental friendly and more suitable for the industrial-scale production. We expand the nature metabolic network of Escherichia coli using metabolic engineering approach for the production of L-ABA. Read More

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https://microbialcellfactories.biomedcentral.com/articles/10
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http://dx.doi.org/10.1186/s12934-019-1095-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6393993PMC
February 2019
3 Reads

Rational strain improvement for surfactin production: enhancing the yield and generating novel structures.

Microb Cell Fact 2019 Feb 28;18(1):42. Epub 2019 Feb 28.

College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, No. 30 Puzhu South Road, Nanjing, Jiangsu, China.

Surfactin, one of the most powerful microbial surfactants, is a lipopeptide-type biosurfactant which combines interesting physicochemical properties and biological activities. However, the high cost caused by its low productivity largely limits the commercial application of surfactin. Hence, many engineered bacterium have also been used to enhance surfactin biosynthesis. Read More

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http://dx.doi.org/10.1186/s12934-019-1089-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6394072PMC
February 2019
1 Read

Disruption of poly (3-hydroxyalkanoate) depolymerase gene and overexpression of three poly (3-hydroxybutyrate) biosynthetic genes improve poly (3-hydroxybutyrate) production from nitrogen rich medium by Rhodobacter sphaeroides.

Microb Cell Fact 2019 Feb 26;18(1):40. Epub 2019 Feb 26.

Graduate School of Science, Technology and Innovation, Kobe University, 1-1 Rokkodaicho, Nada-ku, Kobe, Hyogo, 657-8501, Japan.

Background: Due to various environmental problems, biodegradable polymers such as poly (3-hydroxybutyrate) (PHB) have gained much attention in recent years. Purple non-sulfur (PNS) bacteria have various attractive characteristics useful for environmentally harmless PHB production. However, production of PHB by PNS bacteria using genetic engineering has never been reported. Read More

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http://dx.doi.org/10.1186/s12934-019-1088-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6390342PMC
February 2019
1 Read

Metabolic engineering of microorganisms for production of aromatic compounds.

Microb Cell Fact 2019 Feb 26;18(1):41. Epub 2019 Feb 26.

Metabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical and Biomolecular Engineering (BK21 Plus Program) and Institute for the BioCentury, Korea Advanced Institute of Science and Technology (KAIST), Daejeon, 34141, Republic of Korea.

Metabolic engineering has been enabling development of high performance microbial strains for the efficient production of natural and non-natural compounds from renewable non-food biomass. Even though microbial production of various chemicals has successfully been conducted and commercialized, there are still numerous chemicals and materials that await their efficient bio-based production. Aromatic chemicals, which are typically derived from benzene, toluene and xylene in petroleum industry, have been used in large amounts in various industries. Read More

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https://microbialcellfactories.biomedcentral.com/articles/10
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http://dx.doi.org/10.1186/s12934-019-1090-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6390333PMC
February 2019
3 Reads

Engineering of leucine-responsive regulatory protein improves spiramycin and bitespiramycin biosynthesis.

Microb Cell Fact 2019 Feb 19;18(1):38. Epub 2019 Feb 19.

NHC Key Laboratory of Biotechnology of Antibiotics, Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences, No. 1 Tian Tan Xi Li, Beijing, 100050, People's Republic of China.

Background: Bitespiramycin (BT) is produced by recombinant spiramycin (SP) producing strain Streptomyces spiramyceticus harboring a heterologous 4″-O-isovaleryltransferase gene (ist). Exogenous L-Leucine (L-Leu) could improve the production of BT. The orf2 gene found from the genomic sequence of S. Read More

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http://dx.doi.org/10.1186/s12934-019-1086-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6379999PMC
February 2019

Electricigens in the anode of microbial fuel cells: pure cultures versus mixed communities.

Microb Cell Fact 2019 Feb 19;18(1):39. Epub 2019 Feb 19.

CAS Key Laboratory of Biobased Materials, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, 266101, China.

Microbial fuel cell (MFC) is an environmentally friendly technology for electricity harvesting from a variety of substrates. Microorganisms used as catalysts in the anodic chamber, which are termed as electricigens, play a major role in the operation of MFCs. This review provides an introduction to the currently identified electricigens on their taxonomical groups and electricity producing abilities. Read More

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http://dx.doi.org/10.1186/s12934-019-1087-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6380051PMC
February 2019
1 Read

Correction to: Triauxic growth of an oleaginous red yeast Rhodosporidium toruloides on waste 'extract' for enhanced and concomitant lipid and β‑carotene production.

Microb Cell Fact 2019 Feb 18;18(1):37. Epub 2019 Feb 18.

Amity Institute of Biotechnology, Amity University, Sec 125, Noida, Uttar Pradesh, 201313, India.

Upon publication of this article [1], the authors realized that one of the coauthor name was accedentally deleted during the review process and not presented in the original version of the article. The coauthor Dr. Ram Prasad should be listed as fifth author. Read More

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http://dx.doi.org/10.1186/s12934-019-1082-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6378736PMC
February 2019
7 Reads
4.221 Impact Factor

Cell factories converting lactate and acetate to butyrate: Clostridium butyricum and microbial communities from dark fermentation bioreactors.

Microb Cell Fact 2019 Feb 13;18(1):36. Epub 2019 Feb 13.

Institute of Biochemistry and Biophysics - Polish Academy of Sciences, Pawińskiego 5a, 02-106, Warsaw, Poland.

Background: Interactions between microorganisms during specific steps of anaerobic digestion determine metabolic pathways in bioreactors and consequently the efficiency of fermentation processes. This study focuses on conversion of lactate and acetate to butyrate by bacteria of dark fermentation. The recently recognized flavin-based electron bifurcation as a mode of energy coupling by anaerobes increases our knowledge of anaerobic lactate oxidation and butyrate formation. Read More

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http://dx.doi.org/10.1186/s12934-019-1085-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6373154PMC
February 2019

Engineering microbial consortia by division of labor.

Microb Cell Fact 2019 Feb 8;18(1):35. Epub 2019 Feb 8.

Department of Energy, Environmental and Chemical Engineering, Washington University, Saint Louis, MO, 63130, USA.

During microbial applications, metabolic burdens can lead to a significant drop in cell performance. Novel synthetic biology tools or multi-step bioprocessing (e.g. Read More

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https://microbialcellfactories.biomedcentral.com/articles/10
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http://dx.doi.org/10.1186/s12934-019-1083-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6368712PMC
February 2019
10 Reads

Large-scale production of tauroursodeoxycholic acid products through fermentation optimization of engineered Escherichia coli cell factory.

Microb Cell Fact 2019 Feb 8;18(1):34. Epub 2019 Feb 8.

The SATCM Key Laboratory for New Resources & Quality Evaluation of Chinese Medicine, The MOE Key Laboratory for Standardization of Chinese Medicines and Shanghai Key Laboratory of Compound Chinese Medicines, Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine, Shanghai, 201203, People's Republic of China.

Background: Bear bile powder is a valuable medicinal material characterized by high content of tauroursodeoxycholic acid (TUDCA) at a certain ratio to taurochenodeoxycholic acid (TCDCA). We had created an engineered E. coli harboring two-step bidirectional oxidative and reductive enzyme-catalyzing pathway that could rapidly convert TCDCA to TUDCA at a specific percentage in shake flasks. Read More

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http://dx.doi.org/10.1186/s12934-019-1076-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6368744PMC
February 2019
4.221 Impact Factor

Establishment of BmoR-based biosensor to screen isobutanol overproducer.

Microb Cell Fact 2019 Feb 7;18(1):30. Epub 2019 Feb 7.

Key Laboratory of Molecular Medicine and Biotherapy, School of Life Science, Beijing Institute of Technology, No. 5 South Zhongguancun Street, Beijing, 100081, China.

Background: Isobutanol, a C4 branched-chain higher alcohol, is regarded as an attractive next-generation transport fuel. Metabolic engineering for efficient isobutanol production has been achieved in many studies. BmoR, an alcohol-regulated transcription factor, mediates a σ-dependent promoter P of alkane monooxygenase in n-alkane metabolism of Thauera butanivorans and displays high sensitivity to C4-C6 linear alcohols and C3-C5 branched-chain alcohols. Read More

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http://dx.doi.org/10.1186/s12934-019-1084-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6366067PMC
February 2019
2 Reads
4.221 Impact Factor

Direct pathway cloning of the sodorifen biosynthetic gene cluster and recombinant generation of its product in E. coli.

Microb Cell Fact 2019 Feb 7;18(1):32. Epub 2019 Feb 7.

Biosystems Chemistry, Department of Chemistry and Center for Integrated Protein Science Munich (CIPSM), Technical University of Munich, Lichtenbergstraße 4, 85748, Garching, Germany.

Background: Serratia plymuthica WS3236 was selected for whole genome sequencing based on preliminary genetic and chemical screening indicating the presence of multiple natural product pathways. This led to the identification of a putative sodorifen biosynthetic gene cluster (BGC). The natural product sodorifen is a volatile organic compound (VOC) with an unusual polymethylated hydrocarbon bicyclic structure (CH) produced by selected strains of S. Read More

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http://dx.doi.org/10.1186/s12934-019-1080-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6366047PMC
February 2019

Microbial production of lipid-protein vesicles using enveloped bacteriophage phi6.

Microb Cell Fact 2019 Feb 7;18(1):29. Epub 2019 Feb 7.

Molecular and Integrative Biosciences Research Programme, Faculty of Biological and Environmental Sciences, University of Helsinki, 00014, Helsinki, Finland.

Background: Cystoviruses have a phospholipid envelope around their nucleocapsid. Such a feature is unique among bacterial viruses (i.e. Read More

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http://dx.doi.org/10.1186/s12934-019-1079-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6366064PMC
February 2019
1 Read

The YoaW signal peptide directs efficient secretion of different heterologous proteins fused to a StrepII-SUMO tag in Bacillus subtilis.

Microb Cell Fact 2019 Feb 7;18(1):31. Epub 2019 Feb 7.

Department of Microbiology, Faculty of Natural and Environmental Sciences, University of Applied Sciences Zittau/Görlitz, Theodor-Körner-Allee 16, 02763, Zittau, Germany.

Background: Heterologous gene expression is well established for various prokaryotic model systems. However, low yield, incorrect folding and instability still impede the production of soluble, bioactive proteins. To improve protein production with the Gram-positive host Bacillus subtilis, a secretory expression system was designed that enhances translocation, folding and stability of heterologous proteins, and simplifies purification. Read More

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http://dx.doi.org/10.1186/s12934-019-1078-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6366066PMC
February 2019
2 Reads

Tailoring the properties of (catalytically)-active inclusion bodies.

Microb Cell Fact 2019 Feb 7;18(1):33. Epub 2019 Feb 7.

Institut für Molekulare Enzymtechnologie, Heinrich-Heine-Universität Düsseldorf, Forschungszentrum Jülich, 52425, Jülich, Germany.

Background: Immobilization is an appropriate tool to ease the handling and recycling of enzymes in biocatalytic processes and to increase their stability. Most of the established immobilization methods require case-to-case optimization, which is laborious and time-consuming. Often, (chromatographic) enzyme purification is required and stable immobilization usually includes additional cross-linking or adsorption steps. Read More

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http://dx.doi.org/10.1186/s12934-019-1081-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6367779PMC
February 2019

Systems metabolic engineering for citric acid production by Aspergillus niger in the post-genomic era.

Microb Cell Fact 2019 Feb 4;18(1):28. Epub 2019 Feb 4.

Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, 300308, People's Republic of China.

Citric acid is the world's largest consumed organic acid and is widely used in beverage, food and pharmaceutical industries. Aspergillus niger is the main industrial workhorse for citric acid production. Since the release of the genome sequence, extensive multi-omic data are being rapidly obtained, which greatly boost our understanding of the citric acid accumulation mechanism in A. Read More

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http://dx.doi.org/10.1186/s12934-019-1064-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6362574PMC
February 2019

Production of L-alanyl-L-glutamine by immobilized Pichia pastoris GS115 expressing α-amino acid ester acyltransferase.

Microb Cell Fact 2019 Feb 2;18(1):27. Epub 2019 Feb 2.

School of Life Science and Biotechnology, Shanghai Jiaotong University, Shanghai, 200240, China.

Background: L-Alanyl-L-glutamine (Ala-Gln) represents the great application potential in clinic due to the unique physicochemical properties. A new approach was developed to synthesize Ala-Gln by recombinant Escherichia coli OPA, which could overcome the disadvantages of traditional chemical synthesis. Although satisfactory results had been obtained with recombinant E. Read More

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http://dx.doi.org/10.1186/s12934-019-1077-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6359838PMC
February 2019
1 Read

Growth-dependent recombinant product formation kinetics can be reproduced through engineering of glucose transport and is prone to phenotypic heterogeneity.

Microb Cell Fact 2019 Feb 2;18(1):26. Epub 2019 Feb 2.

Departamento de Ingeniería Celular y Biocatálisis, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Cuernavaca, Morelos, Mexico.

Background: Escherichia coli W3110 and a group of six isogenic derivatives, each displaying distinct specific rates of glucose consumption were characterized to determine levels of GFP production and population heterogeneity. These strains have single or combinatory deletions in genes encoding phosphoenolpyruvate:sugar phosphotransferase system (PTS) permeases as PtsG and ManX, as well as common components EI, Hpr protein and EIIA, also the non-PTS Mgl galactose/glucose ABC transporter. They have been transformed for expressing GFP based on a lac-based expression vector, which is subject to bistability. Read More

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http://dx.doi.org/10.1186/s12934-019-1073-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6359759PMC
February 2019

Release of glucose repression on xylose utilization in Kluyveromyces marxianus to enhance glucose-xylose co-utilization and xylitol production from corncob hydrolysate.

Microb Cell Fact 2019 Feb 1;18(1):24. Epub 2019 Feb 1.

School of Life Sciences, University of Science and Technology of China, Hefei, 230027, Anhui, People's Republic of China.

Background: Lignocellulosic biomass is one of the most abundant materials for biochemicals production. However, efficient co-utilization of glucose and xylose from the lignocellulosic biomass is a challenge due to the glucose repression in microorganisms. Kluyveromyces marxianus is a thermotolerant and efficient xylose-utilizing yeast. Read More

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http://dx.doi.org/10.1186/s12934-019-1068-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6359873PMC
February 2019
2 Reads

Heterologous phosphoketolase expression redirects flux towards acetate, perturbs sugar phosphate pools and increases respiratory demand in Saccharomyces cerevisiae.

Microb Cell Fact 2019 Feb 1;18(1):25. Epub 2019 Feb 1.

Department of Biology and Biological Engineering, Chalmers University of Technology, Kemivägen 10, 41296, Gothenburg, Sweden.

Introduction: Phosphoketolases (Xfpk) are a non-native group of enzymes in yeast, which can be expressed in combination with other metabolic enzymes to positively influence the yield of acetyl-CoA derived products by reducing carbon losses in the form of CO. In this study, a yeast strain expressing Xfpk from Bifidobacterium breve, which was previously found to have a growth defect and to increase acetate production, was characterized.

Results: Xfpk-expression was found to increase respiration and reduce biomass yield during glucose consumption in batch and chemostat cultivations. Read More

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http://dx.doi.org/10.1186/s12934-019-1072-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6359841PMC
February 2019
4 Reads

Exploring natural biodiversity to expand access to microbial terpene synthesis.

Microb Cell Fact 2019 Feb 1;18(1):23. Epub 2019 Feb 1.

Aix-Marseille Univ, CNRS, Centrale Marseille, iSm2, Marseille, France.

Background: Terpenes are industrially relevant natural compounds the biosynthesis of which relies on two well-established-mevalonic acid (MVA) and methyl erythritol phosphate (MEP)-pathways. Both pathways are widely distributed in all domains of life, the former is predominantly found in eukaryotes and archaea and the latter in eubacteria and chloroplasts. These two pathways supply isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP), the universal building blocks of terpenes. Read More

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http://dx.doi.org/10.1186/s12934-019-1074-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6359773PMC
February 2019