1,866 results match your criteria Microbial Cell Factories [Journal]


Cell factories converting lactate and acetate to butyrate: Clostridium butyricum and microbial communities from dark fermentation bioreactors.

Microb Cell Fact 2019 Feb 13;18(1):36. Epub 2019 Feb 13.

Institute of Biochemistry and Biophysics - Polish Academy of Sciences, Pawińskiego 5a, 02-106, Warsaw, Poland.

Background: Interactions between microorganisms during specific steps of anaerobic digestion determine metabolic pathways in bioreactors and consequently the efficiency of fermentation processes. This study focuses on conversion of lactate and acetate to butyrate by bacteria of dark fermentation. The recently recognized flavin-based electron bifurcation as a mode of energy coupling by anaerobes increases our knowledge of anaerobic lactate oxidation and butyrate formation. Read More

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http://dx.doi.org/10.1186/s12934-019-1085-1DOI Listing
February 2019

Engineering microbial consortia by division of labor.

Microb Cell Fact 2019 Feb 8;18(1):35. Epub 2019 Feb 8.

Department of Energy, Environmental and Chemical Engineering, Washington University, Saint Louis, MO, 63130, USA.

During microbial applications, metabolic burdens can lead to a significant drop in cell performance. Novel synthetic biology tools or multi-step bioprocessing (e.g. Read More

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https://microbialcellfactories.biomedcentral.com/articles/10
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http://dx.doi.org/10.1186/s12934-019-1083-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6368712PMC
February 2019
2 Reads

Large-scale production of tauroursodeoxycholic acid products through fermentation optimization of engineered Escherichia coli cell factory.

Microb Cell Fact 2019 Feb 8;18(1):34. Epub 2019 Feb 8.

The SATCM Key Laboratory for New Resources & Quality Evaluation of Chinese Medicine, The MOE Key Laboratory for Standardization of Chinese Medicines and Shanghai Key Laboratory of Compound Chinese Medicines, Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine, Shanghai, 201203, People's Republic of China.

Background: Bear bile powder is a valuable medicinal material characterized by high content of tauroursodeoxycholic acid (TUDCA) at a certain ratio to taurochenodeoxycholic acid (TCDCA). We had created an engineered E. coli harboring two-step bidirectional oxidative and reductive enzyme-catalyzing pathway that could rapidly convert TCDCA to TUDCA at a specific percentage in shake flasks. Read More

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http://dx.doi.org/10.1186/s12934-019-1076-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6368744PMC
February 2019

Establishment of BmoR-based biosensor to screen isobutanol overproducer.

Microb Cell Fact 2019 Feb 7;18(1):30. Epub 2019 Feb 7.

Key Laboratory of Molecular Medicine and Biotherapy, School of Life Science, Beijing Institute of Technology, No. 5 South Zhongguancun Street, Beijing, 100081, China.

Background: Isobutanol, a C4 branched-chain higher alcohol, is regarded as an attractive next-generation transport fuel. Metabolic engineering for efficient isobutanol production has been achieved in many studies. BmoR, an alcohol-regulated transcription factor, mediates a σ-dependent promoter P of alkane monooxygenase in n-alkane metabolism of Thauera butanivorans and displays high sensitivity to C4-C6 linear alcohols and C3-C5 branched-chain alcohols. Read More

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http://dx.doi.org/10.1186/s12934-019-1084-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6366067PMC
February 2019
4.221 Impact Factor

Direct pathway cloning of the sodorifen biosynthetic gene cluster and recombinant generation of its product in E. coli.

Microb Cell Fact 2019 Feb 7;18(1):32. Epub 2019 Feb 7.

Biosystems Chemistry, Department of Chemistry and Center for Integrated Protein Science Munich (CIPSM), Technical University of Munich, Lichtenbergstraße 4, 85748, Garching, Germany.

Background: Serratia plymuthica WS3236 was selected for whole genome sequencing based on preliminary genetic and chemical screening indicating the presence of multiple natural product pathways. This led to the identification of a putative sodorifen biosynthetic gene cluster (BGC). The natural product sodorifen is a volatile organic compound (VOC) with an unusual polymethylated hydrocarbon bicyclic structure (CH) produced by selected strains of S. Read More

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http://dx.doi.org/10.1186/s12934-019-1080-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6366047PMC
February 2019

Microbial production of lipid-protein vesicles using enveloped bacteriophage phi6.

Microb Cell Fact 2019 Feb 7;18(1):29. Epub 2019 Feb 7.

Molecular and Integrative Biosciences Research Programme, Faculty of Biological and Environmental Sciences, University of Helsinki, 00014, Helsinki, Finland.

Background: Cystoviruses have a phospholipid envelope around their nucleocapsid. Such a feature is unique among bacterial viruses (i.e. Read More

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http://dx.doi.org/10.1186/s12934-019-1079-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6366064PMC
February 2019

The YoaW signal peptide directs efficient secretion of different heterologous proteins fused to a StrepII-SUMO tag in Bacillus subtilis.

Microb Cell Fact 2019 Feb 7;18(1):31. Epub 2019 Feb 7.

Department of Microbiology, Faculty of Natural and Environmental Sciences, University of Applied Sciences Zittau/Görlitz, Theodor-Körner-Allee 16, 02763, Zittau, Germany.

Background: Heterologous gene expression is well established for various prokaryotic model systems. However, low yield, incorrect folding and instability still impede the production of soluble, bioactive proteins. To improve protein production with the Gram-positive host Bacillus subtilis, a secretory expression system was designed that enhances translocation, folding and stability of heterologous proteins, and simplifies purification. Read More

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http://dx.doi.org/10.1186/s12934-019-1078-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6366066PMC
February 2019
1 Read

Tailoring the properties of (catalytically)-active inclusion bodies.

Microb Cell Fact 2019 Feb 7;18(1):33. Epub 2019 Feb 7.

Institut für Molekulare Enzymtechnologie, Heinrich-Heine-Universität Düsseldorf, Forschungszentrum Jülich, 52425, Jülich, Germany.

Background: Immobilization is an appropriate tool to ease the handling and recycling of enzymes in biocatalytic processes and to increase their stability. Most of the established immobilization methods require case-to-case optimization, which is laborious and time-consuming. Often, (chromatographic) enzyme purification is required and stable immobilization usually includes additional cross-linking or adsorption steps. Read More

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http://dx.doi.org/10.1186/s12934-019-1081-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6367779PMC
February 2019

Systems metabolic engineering for citric acid production by Aspergillus niger in the post-genomic era.

Microb Cell Fact 2019 Feb 4;18(1):28. Epub 2019 Feb 4.

Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, 300308, People's Republic of China.

Citric acid is the world's largest consumed organic acid and is widely used in beverage, food and pharmaceutical industries. Aspergillus niger is the main industrial workhorse for citric acid production. Since the release of the genome sequence, extensive multi-omic data are being rapidly obtained, which greatly boost our understanding of the citric acid accumulation mechanism in A. Read More

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http://dx.doi.org/10.1186/s12934-019-1064-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6362574PMC
February 2019

Production of L-alanyl-L-glutamine by immobilized Pichia pastoris GS115 expressing α-amino acid ester acyltransferase.

Microb Cell Fact 2019 Feb 2;18(1):27. Epub 2019 Feb 2.

School of Life Science and Biotechnology, Shanghai Jiaotong University, Shanghai, 200240, China.

Background: L-Alanyl-L-glutamine (Ala-Gln) represents the great application potential in clinic due to the unique physicochemical properties. A new approach was developed to synthesize Ala-Gln by recombinant Escherichia coli OPA, which could overcome the disadvantages of traditional chemical synthesis. Although satisfactory results had been obtained with recombinant E. Read More

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http://dx.doi.org/10.1186/s12934-019-1077-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6359838PMC
February 2019

Growth-dependent recombinant product formation kinetics can be reproduced through engineering of glucose transport and is prone to phenotypic heterogeneity.

Microb Cell Fact 2019 Feb 2;18(1):26. Epub 2019 Feb 2.

Departamento de Ingeniería Celular y Biocatálisis, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Cuernavaca, Morelos, Mexico.

Background: Escherichia coli W3110 and a group of six isogenic derivatives, each displaying distinct specific rates of glucose consumption were characterized to determine levels of GFP production and population heterogeneity. These strains have single or combinatory deletions in genes encoding phosphoenolpyruvate:sugar phosphotransferase system (PTS) permeases as PtsG and ManX, as well as common components EI, Hpr protein and EIIA, also the non-PTS Mgl galactose/glucose ABC transporter. They have been transformed for expressing GFP based on a lac-based expression vector, which is subject to bistability. Read More

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http://dx.doi.org/10.1186/s12934-019-1073-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6359759PMC
February 2019

Release of glucose repression on xylose utilization in Kluyveromyces marxianus to enhance glucose-xylose co-utilization and xylitol production from corncob hydrolysate.

Microb Cell Fact 2019 Feb 1;18(1):24. Epub 2019 Feb 1.

School of Life Sciences, University of Science and Technology of China, Hefei, 230027, Anhui, People's Republic of China.

Background: Lignocellulosic biomass is one of the most abundant materials for biochemicals production. However, efficient co-utilization of glucose and xylose from the lignocellulosic biomass is a challenge due to the glucose repression in microorganisms. Kluyveromyces marxianus is a thermotolerant and efficient xylose-utilizing yeast. Read More

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http://dx.doi.org/10.1186/s12934-019-1068-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6359873PMC
February 2019

Heterologous phosphoketolase expression redirects flux towards acetate, perturbs sugar phosphate pools and increases respiratory demand in Saccharomyces cerevisiae.

Microb Cell Fact 2019 Feb 1;18(1):25. Epub 2019 Feb 1.

Department of Biology and Biological Engineering, Chalmers University of Technology, Kemivägen 10, 41296, Gothenburg, Sweden.

Introduction: Phosphoketolases (Xfpk) are a non-native group of enzymes in yeast, which can be expressed in combination with other metabolic enzymes to positively influence the yield of acetyl-CoA derived products by reducing carbon losses in the form of CO. In this study, a yeast strain expressing Xfpk from Bifidobacterium breve, which was previously found to have a growth defect and to increase acetate production, was characterized.

Results: Xfpk-expression was found to increase respiration and reduce biomass yield during glucose consumption in batch and chemostat cultivations. Read More

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http://dx.doi.org/10.1186/s12934-019-1072-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6359841PMC
February 2019
1 Read

Exploring natural biodiversity to expand access to microbial terpene synthesis.

Microb Cell Fact 2019 Feb 1;18(1):23. Epub 2019 Feb 1.

Aix-Marseille Univ, CNRS, Centrale Marseille, iSm2, Marseille, France.

Background: Terpenes are industrially relevant natural compounds the biosynthesis of which relies on two well-established-mevalonic acid (MVA) and methyl erythritol phosphate (MEP)-pathways. Both pathways are widely distributed in all domains of life, the former is predominantly found in eukaryotes and archaea and the latter in eubacteria and chloroplasts. These two pathways supply isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP), the universal building blocks of terpenes. Read More

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http://dx.doi.org/10.1186/s12934-019-1074-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6359773PMC
February 2019

Neutralizing-antibody-mediated protection of chickens against infectious bursal disease via one-time vaccination with inactivated recombinant Lactococcus lactis expressing a fusion protein constructed from the RCK protein of Salmonella enterica and VP2 of infectious bursal disease virus.

Microb Cell Fact 2019 Jan 31;18(1):21. Epub 2019 Jan 31.

State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, 150069, China.

Background: Infectious bursal disease (IBD) is an acute contagious immunosuppressive disease which lead to acute bursal injury and immune dysfunction in poultry. It has caused heavy economic losses in the commercial poultry industry for many years in worldwide. Attenuated live vaccine has widely used in poultry showing some promising signs against IBDV infection. Read More

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http://dx.doi.org/10.1186/s12934-019-1061-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6357496PMC
January 2019
4.221 Impact Factor

A rapid and versatile tool for genomic engineering in Lactococcus lactis.

Microb Cell Fact 2019 Jan 31;18(1):22. Epub 2019 Jan 31.

State Key Laboratory of Microbial Technology, Shandong University, No. 72 Binhai Road, Qingdao, 266237, People's Republic of China.

Background: Lactococcus lactis is one of the most extensively characterized lactic acid bacteria, from physiological traits to industrial exploitation. Since last decade, L. lactis has been developed into cell factories for the production of bioactive compounds such as enzymes, vaccine antigens and natural products. Read More

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https://microbialcellfactories.biomedcentral.com/articles/10
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http://dx.doi.org/10.1186/s12934-019-1075-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6357491PMC
January 2019
2 Reads
4.221 Impact Factor

Production of long-chain free fatty acids from metabolically engineered Rhodobacter sphaeroides heterologously producing periplasmic phospholipase A2 in dodecane-overlaid two-phase culture.

Microb Cell Fact 2019 Jan 31;18(1):20. Epub 2019 Jan 31.

Department of Life Science, Sogang University, Mapo, Shinsu 1, Seoul, 121-742, South Korea.

Background: Long-chain free fatty acids (FFAs) are a type of backbone molecule that can react with alcohol to produce biodiesels. Various microorganisms have become potent producers of FFAs. Efforts have focused on increasing metabolic flux to the synthesis of either neutral fat or fatty acyl intermediates attached to acyl carrier protein (ACP), which are the source of FFAs. Read More

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http://dx.doi.org/10.1186/s12934-019-1070-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6357386PMC
January 2019

Comparative proteome analysis in an Escherichia coli CyDisCo strain identifies stress responses related to protein production, oxidative stress and accumulation of misfolded protein.

Microb Cell Fact 2019 Jan 29;18(1):19. Epub 2019 Jan 29.

School of Biosciences, University of Kent, Canterbury, CT2 7NJ, UK.

Background: The Twin-arginine translocation (Tat) pathway of Escherichia coli has great potential for the export of biopharmaceuticals to the periplasm due to its ability to transport folded proteins, and its proofreading mechanism that allows correctly folded proteins to translocate. Coupling the Tat-dependent protein secretion with the formation of disulfide bonds in the cytoplasm of E. coli CyDisCo provides a powerful platform for the production of industrially challenging proteins. Read More

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http://dx.doi.org/10.1186/s12934-019-1071-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6350376PMC
January 2019
2 Reads

Biosensor-guided improvements in salicylate production by recombinant Escherichia coli.

Microb Cell Fact 2019 Jan 29;18(1):18. Epub 2019 Jan 29.

Department of Chemical & Biomolecular Engineering, University of Houston, S222 Engineering Building 1, Houston, TX, 77204-4004, USA.

Background: Salicylate can be biosynthesized from the common metabolic intermediate shikimate and has found applications in pharmaceuticals and in the bioplastics industry. While much metabolic engineering work focused on the shikimate pathway has led to the biosynthesis of a variety of aromatic compounds, little is known about how the relative expression levels of pathway components influence salicylate biosynthesis. Furthermore, some host strain gene deletions that improve salicylate production may be impossible to predict. Read More

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http://dx.doi.org/10.1186/s12934-019-1069-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6350385PMC
January 2019

Rational construction of genome-reduced and high-efficient industrial Streptomyces chassis based on multiple comparative genomic approaches.

Microb Cell Fact 2019 Jan 28;18(1):16. Epub 2019 Jan 28.

Institute of Pharmaceutical Biotechnology & First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, 310058, China.

Background: Streptomyces chattanoogensis L10 is the industrial producer of natamycin and has been proved a highly efficient host for diverse natural products. It has an enormous potential to be developed as a versatile cell factory for production of heterologous secondary metabolites. Here we developed a genome-reduced industrial Streptomyces chassis by rational 'design-build-test' pipeline. Read More

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http://dx.doi.org/10.1186/s12934-019-1055-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6348691PMC
January 2019

Carbon sources and XlnR-dependent transcriptional landscape of CAZymes in the industrial fungus Talaromyces versatilis: when exception seems to be the rule.

Microb Cell Fact 2019 Jan 28;18(1):14. Epub 2019 Jan 28.

LISBP, Université de Toulouse, INSA, INRA, CNRS, Toulouse, France.

Background: Research on filamentous fungi emphasized the remarkable redundancy in genes encoding hydrolytic enzymes, the similarities but also the large differences in their expression, especially through the role of the XlnR/XYR1 transcriptional activator. The purpose of this study was to evaluate the specificities of the industrial fungus Talaromyces versatilis, getting clues into the role of XlnR and the importance of glucose repression at the transcriptional level, to provide further levers for cocktail production.

Results: By studying a set of 62 redundant genes representative of several categories of enzymes, our results underlined the huge plasticity of transcriptional responses when changing nutritional status. Read More

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http://dx.doi.org/10.1186/s12934-019-1062-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6348686PMC
January 2019

High-efficiency expression and secretion of human FGF21 in Bacillus subtilis by intercalation of a mini-cistron cassette and combinatorial optimization of cell regulatory components.

Microb Cell Fact 2019 Jan 28;18(1):17. Epub 2019 Jan 28.

Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, 300308, People's Republic of China.

Background: Recombinant human Fibroblast growth factor 21 (rhFGF21) is an endocrine hormone that has profound effects on treatment of metabolic diseases. However, rhFGF21 is prone to form inclusion body when expressed in bacteria, which results in, the downstream process of purification of bioactive rhFGF21 is time-consuming and labor intensive. The aim of this work is to explore a new method for improving the soluble expression and secretion level of rhFGF21 in B. Read More

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http://dx.doi.org/10.1186/s12934-019-1066-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6348689PMC
January 2019

Engineering an electroactive Escherichia coli for the microbial electrosynthesis of succinate from glucose and CO.

Microb Cell Fact 2019 Jan 28;18(1):15. Epub 2019 Jan 28.

Key Laboratory of Systems Microbial Biotechnology, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, 32 West 7th Ave, Tianjin Airport Economic Park, Tianjin, 300308, China.

Background: Electrochemical energy is a key factor of biosynthesis, and is necessary for the reduction or assimilation of substrates such as CO. Previous microbial electrosynthesis (MES) research mainly utilized naturally electroactive microbes to generate non-specific products.

Results: In this research, an electroactive succinate-producing cell factory was engineered in E. Read More

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http://dx.doi.org/10.1186/s12934-019-1067-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6348651PMC
January 2019
4.221 Impact Factor

Asp305Gly mutation improved the activity and stability of the styrene monooxygenase for efficient epoxide production in Pseudomonas putida KT2440.

Microb Cell Fact 2019 Jan 24;18(1):12. Epub 2019 Jan 24.

The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, 1800 Lihu Road, Wuxi, 214122, Jiangsu, China.

Background: Styrene monooxygenase (SMO) catalyzes the first step of aromatic alkene degradation yielding the corresponding epoxides. Because of its broad spectrum of substrates, the enzyme harbors a great potential for an application in medicine and chemical industries.

Results: In this study, we achieved higher enzymatic activity and better stability towards styrene by enlarging the ligand entrance tunnel and improving the hydrophobicity through error-prone PCR and site-saturation mutagenesis. Read More

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https://microbialcellfactories.biomedcentral.com/articles/10
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http://dx.doi.org/10.1186/s12934-019-1065-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6345017PMC
January 2019
2 Reads

Genomic sequencing, genome-scale metabolic network reconstruction, and in silico flux analysis of the grape endophytic fungus Alternaria sp. MG1.

Microb Cell Fact 2019 Jan 24;18(1):13. Epub 2019 Jan 24.

Key Laboratory for Space Bioscience and Biotechnology, School of Life Sciences, Northwestern Polytechnical University, 127 Youyi West Road, Xi'an, 710072, Shaanxi, China.

Background: Alternaria sp. MG1, an endophytic fungus isolated from grape, is a native producer of resveratrol, which has important application potential. However, the metabolic characteristics and physiological behavior of MG1 still remains mostly unraveled. Read More

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https://microbialcellfactories.biomedcentral.com/articles/10
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http://dx.doi.org/10.1186/s12934-019-1063-7DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6345013PMC
January 2019
2 Reads

Recombinant O-mannosylated protein production (PstS-1) from Mycobacterium tuberculosis in Pichia pastoris (Komagataella phaffii) as a tool to study tuberculosis infection.

Microb Cell Fact 2019 Jan 19;18(1):11. Epub 2019 Jan 19.

Programa de Investigación de Producción de Biomoléculas, Departamento de Biología Molecular y Biotecnología, Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, AP. 70228, CP. 04510, Ciudad de México, Mexico.

Background: Pichia pastoris (syn. Komagataella phaffii) is one of the most highly utilized eukaryotic expression systems for the production of heterologous glycoproteins, being able to perform both N- and O-mannosylation. In this study, we present the expression in P. Read More

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https://microbialcellfactories.biomedcentral.com/articles/10
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http://dx.doi.org/10.1186/s12934-019-1059-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6339365PMC
January 2019
10 Reads

Simultaneous enhancement of the beta-exo synergism and exo-exo synergism in Trichoderma reesei cellulase to increase the cellulose degrading capability.

Microb Cell Fact 2019 Jan 18;18(1). Epub 2019 Jan 18.

College of Life Sciences, Northwest A&F University, 22 Xinong Road, Yangling, 712100, Shaanxi, China.

Background: Cellulase is the one of the largest contributors to the high production costs of the lignocellulose-based biorefineries. As the most widely used cellulase producer, Trichoderma reesei has two weaknesses, deficiencies in β-glucosidase and cellobiohydrolase II. This work aimed at solving this problem by simultaneous enhancement of the beta-exo synergism and exo-exo synergism in T. Read More

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http://dx.doi.org/10.1186/s12934-019-1060-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6337788PMC
January 2019

Engineering the flagellar type III secretion system: improving capacity for secretion of recombinant protein.

Microb Cell Fact 2019 Jan 18;18(1):10. Epub 2019 Jan 18.

Integrated BioSciences, School of Clinical Dentistry, University of Sheffield, Sheffield, S10 2TA, UK.

Background: Many valuable biopharmaceutical and biotechnological proteins have been produced in Escherichia coli, however these proteins are almost exclusively localised in the cytoplasm or periplasm. This presents challenges for purification, i.e. Read More

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http://dx.doi.org/10.1186/s12934-019-1058-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6337784PMC
January 2019
1 Read

Combinatorial approach for improved cyanidin 3-O-glucoside production in Escherichia coli.

Microb Cell Fact 2019 Jan 17;18(1). Epub 2019 Jan 17.

Department of Life Science and Biochemical Engineering, SunMoon University, 70 Sunmoon-ro 221, Tangjeong-myeon, Asan-si, Chungnam, 31460, Republic of Korea.

Background: Multi-monocistronic and multi-variate vectors were designed, built, and tested for the improved production of cyanidin 3-O-glucoside (C3G) in Escherichia coli BL21 (DE3). The synthetic bio-parts were designed in such a way that multiple genes can be assembled using the bio-brick system, and expressed under different promoters in a single vector. The vectors harbor compatible cloning sites, so that the genes can be shuffled from one vector to another in a single step, and assembled into a single vector. Read More

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http://dx.doi.org/10.1186/s12934-019-1056-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6335687PMC
January 2019
4.221 Impact Factor

Protein production in Escherichia coli is guided by the trade-off between intracellular substrate availability and energy cost.

Microb Cell Fact 2019 Jan 17;18(1). Epub 2019 Jan 17.

Institute of Biochemical Engineering, University of Stuttgart, Stuttgart, Germany.

Background: In vivo protein formation is a crucial part of cellular life. The process needs to adapt to growth conditions and is exploited for the production of technical and pharmaceutical proteins in microbes such as Escherichia coli. Accordingly, the elucidation of basic regulatory mechanisms controlling the in vivo translation machinery is of primary interest, not only to improve heterologous protein production but also to elucidate fundamental regulation regimens of cellular growth. Read More

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http://dx.doi.org/10.1186/s12934-019-1057-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6337870PMC
January 2019
1 Read

Metabolic engineering of Escherichia coli carrying the hybrid acetone-biosynthesis pathway for efficient acetone biosynthesis from acetate.

Microb Cell Fact 2019 Jan 14;18(1). Epub 2019 Jan 14.

State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, Shanghai, 200237, China.

Background: The shortage of food based feedstocks has been one of the stumbling blocks in industrial biomanufacturing. The acetone bioproduction from the traditional acetone-butanol-ethanol fermentation is limited by the non-specificity of products and competitive utilization of food-based substrates. Using genetically modified Escherichia coli to produce acetone as sole product from the cost-effective non-food based substrates showed great potential to overcome these problems. Read More

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http://dx.doi.org/10.1186/s12934-019-1054-8DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6330746PMC
January 2019

Evaluation of scFv protein recovery from E. coli by in vitro refolding and mild solubilization process.

Microb Cell Fact 2019 Jan 14;18(1). Epub 2019 Jan 14.

Faculty of Life Sciences and Biotechnology, South Asian University, New Delhi, India.

Background: The production of therapeutically active single chain variable fragment (scFv) antibody is still challenging in E. coli due to the aggregation propensity of recombinant protein into inclusion bodies (IBs). However, recent advancement of biotechnology has shown substantial recovery of bioactive protein from such insoluble IBs by solubilization and refolding processes. Read More

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http://dx.doi.org/10.1186/s12934-019-1053-9DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6330739PMC
January 2019
2 Reads

Improvement of isoprene production in Escherichia coli by rational optimization of RBSs and key enzymes screening.

Microb Cell Fact 2019 Jan 9;18(1). Epub 2019 Jan 9.

Key Laboratory of Biobased Materials, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, No.135 Songlin Road, Qingdao, 266101, People's Republic of China.

Background: As an essential platform chemical mostly used for rubber synthesis, isoprene is produced in industry through chemical methods, derived from petroleum. As an alternative, bio-production of isoprene has attracted much attention in recent years. Previous researches were mostly focused on key enzymes to improve isoprene production. Read More

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http://dx.doi.org/10.1186/s12934-018-1051-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6327615PMC
January 2019

Integration of enzymatic data in Bacillus subtilis genome-scale metabolic model improves phenotype predictions and enables in silico design of poly-γ-glutamic acid production strains.

Microb Cell Fact 2019 Jan 9;18(1). Epub 2019 Jan 9.

The Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, 2800, Kgs. Lyngby, Denmark.

Background: Genome-scale metabolic models (GEMs) allow predicting metabolic phenotypes from limited data on uptake and secretion fluxes by defining the space of all the feasible solutions and excluding physio-chemically and biologically unfeasible behaviors. The integration of additional biological information in genome-scale models, e.g. Read More

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http://dx.doi.org/10.1186/s12934-018-1052-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6325765PMC
January 2019

Combinatorial pathway enzyme engineering and host engineering overcomes pyruvate overflow and enhances overproduction of N-acetylglucosamine in Bacillus subtilis.

Microb Cell Fact 2019 Jan 4;18(1). Epub 2019 Jan 4.

Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, Jiangnan University, Wuxi, 214122, China.

Background: Glucosamine-6-phosphate N-acetyltransferase (GNA1) is the key enzyme that causes overproduction of N-acetylglucosamine in Bacillus subtilis. Previously, we increased GlcNAc production by promoting the expression of GNA1 from Caenorhabditis elegans (CeGNA1) in an engineered B. subtilis strain BSGN12. Read More

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http://dx.doi.org/10.1186/s12934-018-1049-xDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6318901PMC
January 2019
1 Read

Sustainable biosurfactant produced by Serratia marcescens UCP 1549 and its suitability for agricultural and marine bioremediation applications.

Microb Cell Fact 2019 Jan 4;18(1). Epub 2019 Jan 4.

Nucleus of Research in Environmental Sciences and Biotechnology, Catholic University of Pernambuco, Recife, PE, 50050-590, Brazil.

Background: Biosurfactants are surface-active agents produced by microorganisms that have higher efficiency and stability, lower toxicity and higher biocompatibility and biodegradability than chemical surfactants. Despite its properties and potential application in a wide range of environmental and industrial processes, biosurfactants are still not cost-competitive when compared to their synthetic counterparts. Cost effective technologies and renewable raw substrates as agro-industrial and regional waste from northeast of Brazil as cassava flour wastewater, supplemented with lactose and corn oil are mainly the chemically media for growing microorganism and in turn the production of the biosurfactant of quality. Read More

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http://dx.doi.org/10.1186/s12934-018-1046-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6318876PMC
January 2019

Functional analysis of the role of CcpA in Lactobacillus plantarum grown on fructooligosaccharides or glucose: a transcriptomic perspective.

Microb Cell Fact 2018 Dec 28;17(1):201. Epub 2018 Dec 28.

School of Perfume and Aroma Technology, Shanghai Institute of Technology, Shanghai, 201418, People's Republic of China.

Background: The catabolite control protein A (CcpA) is a master regulator of many important cellular processes in Gram-positive bacteria. In Lactobacillus plantarum, CcpA directly or indirectly controls the transcription of a large number of genes that are involved in carbohydrate metabolism, aerobic and anaerobic growth, stress response and metabolite production, but its role in response to different carbon sources remains unclear.

Results: Here a combined transcriptomic and physiological approach was used to survey the global alterations that occurred during the logarithmic growth phase of wild-type and ccpA mutant strains of L. Read More

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http://dx.doi.org/10.1186/s12934-018-1050-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6309078PMC
December 2018
1 Read

OhsR acts as an organic peroxide-sensing transcriptional activator using an S-mycothiolation mechanism in Corynebacterium glutamicum.

Microb Cell Fact 2018 Dec 26;17(1):200. Epub 2018 Dec 26.

College of Life Sciences, Qufu Normal University, Qufu, 273165, Shandong, China.

Background: Corynebacterium glutamicum is a well-known producer of various L-amino acids in industry. During the fermenting process, C. glutamicum unavoidably encounters oxidative stress due to a specific reactive oxygen species (ROS) produced by consistent adverse conditions. Read More

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http://dx.doi.org/10.1186/s12934-018-1048-yDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6306002PMC
December 2018

Transcriptomic and fluxomic changes in Streptomyces lividans producing heterologous protein.

Microb Cell Fact 2018 Dec 21;17(1):198. Epub 2018 Dec 21.

Department of Chemical Engineering, Bio- and Chemical Systems Technology, Reactor Engineering and Safety Section, KU Leuven, Celestijnenlaan 200F, box 2424, 3001, Leuven, Belgium.

Background: The Gram-positive Streptomyces lividans TK24 is an attractive host for heterologous protein production because of its high capability to secrete proteins-which favors correct folding and facilitates downstream processing-as well as its acceptance of methylated DNA and its low endogeneous protease activity. However, current inconsistencies in protein yields urge for a deeper understanding of the burden of heterologous protein production on the cell. In the current study, transcriptomics and [Formula: see text]-based fluxomics were exploited to uncover gene expression and metabolic flux changes associated with heterologous protein production. Read More

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https://microbialcellfactories.biomedcentral.com/articles/10
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http://dx.doi.org/10.1186/s12934-018-1040-6DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6302529PMC
December 2018
8 Reads

Tuning recombinant protein expression to match secretion capacity.

Microb Cell Fact 2018 Dec 22;17(1):199. Epub 2018 Dec 22.

Manchester Institute of Biotechnology, School of Chemistry, University of Manchester, Manchester, M1 7DN, UK.

Background: The secretion of recombinant disulfide-bond containing proteins into the periplasm of Gram-negative bacterial hosts, such as E. coli, has many advantages that can facilitate product isolation, quality and activity. However, the secretion machinery of E. Read More

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https://microbialcellfactories.biomedcentral.com/articles/10
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http://dx.doi.org/10.1186/s12934-018-1047-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6303999PMC
December 2018
5 Reads

Comparative analysis of deep sequenced methanogenic communities: identification of microorganisms responsible for methane production.

Microb Cell Fact 2018 Dec 20;17(1):197. Epub 2018 Dec 20.

Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Pawinskiego 5a, 02-106, Warsaw, Poland.

Background: Although interactions between microorganisms involved in biogas production are largely uncharted, it is commonly accepted that methanogenic Archaea are essential for the process. Methanogens thrive in various environments, but the most extensively studied communities come from biogas plants. In this study, we employed a metagenomic analysis of deeply sequenced methanogenic communities, which allowed for comparison of taxonomic and functional diversity as well as identification of microorganisms directly involved in various stages of methanogenesis pathways. Read More

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http://dx.doi.org/10.1186/s12934-018-1043-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6302309PMC
December 2018
1 Read

Developing Gram-negative bacteria for the secretion of heterologous proteins.

Microb Cell Fact 2018 Dec 20;17(1):196. Epub 2018 Dec 20.

Department of Chemical and Biological Engineering, Northwestern University, Evanston, USA.

Gram-negative bacteria are attractive hosts for recombinant protein production because they are fast growing, easy to manipulate, and genetically stable in large cultures. However, the utility of these microbes would expand if they also could secrete the product at commercial scales. Secretion of biotechnologically relevant proteins into the extracellular medium increases product purity from cell culture, decreases downstream processing requirements, and reduces overall cost. Read More

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http://dx.doi.org/10.1186/s12934-018-1041-5DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6302416PMC
December 2018

Metabolic engineering of Methylobacterium extorquens AM1 for the production of butadiene precursor.

Microb Cell Fact 2018 Dec 20;17(1):194. Epub 2018 Dec 20.

School of Life Sciences, Shandong Province Key Laboratory of Applied Mycology, and Qingdao International Center on Microbes Utilizing Biogas, Qingdao Agricultural University, Qingdao, Shandong, China.

Background: Butadiene is a platform chemical used as an industrial feedstock for the manufacture of automobile tires, synthetic resins, latex and engineering plastics. Currently, butadiene is predominantly synthesized as a byproduct of ethylene production from non-renewable petroleum resources. Although the idea of biological synthesis of butadiene from sugars has been discussed in the literature, success for that goal has so far not been reported. Read More

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http://dx.doi.org/10.1186/s12934-018-1042-4DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6300920PMC
December 2018
2 Reads

Saccharomyces cerevisiae morphological changes and cytokinesis arrest elicited by hypoxia during scale-up for production of therapeutic recombinant proteins.

Microb Cell Fact 2018 Dec 20;17(1):195. Epub 2018 Dec 20.

Department of Bioanalytical Sciences, Research and Development, GlaxoSmithKline, 709 Swedeland Road, King of Prussia, PA, 19406, USA.

Background: Scaling up of bioprocesses represents a crucial step in the industrial production of biologicals. However, our knowledge about the impact of scale-up on the organism's physiology and function is still incomplete. Our previous studies have suggested the existence of morphological changes during the scale-up of a yeast (Saccharomyces cerevisiae) fermentation process as inferred from the volume fraction occupied by yeast cells and exometabolomics analyses. Read More

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http://dx.doi.org/10.1186/s12934-018-1044-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6300885PMC
December 2018

Production efficiency of the bacterial non-ribosomal peptide indigoidine relies on the respiratory metabolic state in S. cerevisiae.

Microb Cell Fact 2018 Dec 13;17(1):193. Epub 2018 Dec 13.

Biological Systems and Engineering Division, Lawrence Berkeley National Laboratory, Berkeley, CA, 94720, USA.

Background: Beyond pathway engineering, the metabolic state of the production host is critical in maintaining the efficiency of cellular production. The biotechnologically important yeast Saccharomyces cerevisiae adjusts its energy metabolism based on the availability of oxygen and carbon sources. This transition between respiratory and non-respiratory metabolic state is accompanied by substantial modifications of central carbon metabolism, which impact the efficiency of metabolic pathways and the corresponding final product titers. Read More

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https://microbialcellfactories.biomedcentral.com/articles/10
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http://dx.doi.org/10.1186/s12934-018-1045-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6293659PMC
December 2018
6 Reads

One-step affinity purification of fusion proteins with optimal monodispersity and biological activity: application to aggregation-prone HPV E6 proteins.

Microb Cell Fact 2018 Dec 1;17(1):191. Epub 2018 Dec 1.

Équipe Labellisée Ligue 2015, Department of Integrated Structural Biology, Institut de Génétique et de Biologie Moléculaire et Cellulaire (IGBMC), INSERM U1258/CNRS UMR 7104/Université de Strasbourg, 1 rue Laurent Fries, BP 10142, 67404, Illkirch, France.

Background: Bacterial expression and purification of recombinant proteins under homogeneous active form is often challenging. Fusion to highly soluble carrier proteins such as Maltose Binding Protein (MBP) often improves their folding and solubility, but self-association may still occur. For instance, HPV E6 oncoproteins, when produced as MBP-E6 fusions, are expressed as mixtures of biologically inactive oligomers and active monomers. Read More

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http://dx.doi.org/10.1186/s12934-018-1039-zDOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6271572PMC
December 2018

Engineered E. coli W enables efficient 2,3-butanediol production from glucose and sugar beet molasses using defined minimal medium as economic basis.

Microb Cell Fact 2018 Nov 30;17(1):190. Epub 2018 Nov 30.

Institute for Chemical, Environmental and Bioscience Engineering, Research Area Biochemical Engineering, Technische Universität Wien, Gumpendorfer Straße 1a, 1060, Vienna, Austria.

Background: Efficient microbial production of chemicals is often hindered by the cytotoxicity of the products or by the pathogenicity of the host strains. Hence 2,3-butanediol, an important drop-in chemical, is an interesting alternative target molecule for microbial synthesis since it is non-cytotoxic. Metabolic engineering of non-pathogenic and industrially relevant microorganisms, such as Escherichia coli, have already yielded in promising 2,3-butanediol titers showing the potential of microbial synthesis of 2,3-butanediol. Read More

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http://dx.doi.org/10.1186/s12934-018-1038-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6267845PMC
November 2018

A strategy to identify a ketoreductase that preferentially synthesizes pharmaceutically relevant (S)-alcohols using whole-cell biotransformation.

Microb Cell Fact 2018 Dec 3;17(1):192. Epub 2018 Dec 3.

Bugworks Research India, Pvt. Ltd, Bengaluru, India.

Introduction: Chemical industries are constantly in search of an expeditious and environmentally benign method for producing chiral synthons. Ketoreductases have been used as catalysts for enantioselective conversion of desired prochiral ketones to their corresponding alcohol. We chose reported promiscuous ketoreductases belonging to different protein families and expressed them in E. Read More

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http://dx.doi.org/10.1186/s12934-018-1036-2DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6276252PMC
December 2018
4.221 Impact Factor

Highly efficient production of Clostridium cellulolyticum H10 D-psicose 3-epimerase in Bacillus subtilis and use of these cells to produce D-psicose.

Microb Cell Fact 2018 Nov 28;17(1):188. Epub 2018 Nov 28.

State Key Laboratory of Food Science and Technology, Jiangnan University, 1800 Lihu Avenue, Wuxi, 214122, China.

Background: D-Psicose 3-epimerase (DPEase) catalyzes the isomerization of D-fructose to the rare sugar D-psicose, which may help prevent obesity, reduce blood sugar and blood fat, and inhibit intra-abdominal fat accumulation.

Results: In this study, the DPEase of Clostridium cellulolyticum H10 was expressed in the food-grade host Bacillus subtilis. Optimization of the culture medium during shake-flask experiments yielded a DPEase activity of 314 U/mL. Read More

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http://dx.doi.org/10.1186/s12934-018-1037-1DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6260708PMC
November 2018
4.221 Impact Factor

The metabolic switch can be activated in a recombinant strain of Streptomyces lividans by a low oxygen transfer rate in shake flasks.

Microb Cell Fact 2018 Nov 28;17(1):189. Epub 2018 Nov 28.

Programa de Investigación de Producción de Biomoléculas, Unidad de Bioprocesos, Departamento de Biología Molecular y Biotecnología, Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, AP. 70228, CP. 04510, Ciudad de México, Mexico.

Background: In Streptomyces, understanding the switch from primary to secondary metabolism is important for maximizing the production of secondary metabolites such as antibiotics, as well as for optimizing recombinant glycoprotein production. Differences in Streptomyces lividans bacterial aggregation as well as recombinant glycoprotein production and O-mannosylation have been reported due to modifications in the shake flask design. We hypothetized that such differences are related to the metabolic switch that occurs under oxygen-limiting conditions in the cultures. Read More

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http://dx.doi.org/10.1186/s12934-018-1035-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6260694PMC
November 2018