87,427 results match your criteria Microarray Technologies in the Diagnosis and Treatment of Head and Neck Cancer


A Female Patient with Xq28 Microduplication Presenting with Myotubular Myopathy, Confirmed with a Custom-Designed X-array.

Neuropediatrics 2018 Dec 12. Epub 2018 Dec 12.

Department of Medical Genetics, Medical School, National and Kapodistrian University of Athens, Athens, Greece.

X-linked myotubular myopathy (XLMTM) is a rare inherited neuromuscular disorder associated with mutations in the gene on the Xq28 region. We report a severely affected girl with XLMTM, caused by maternally inherited 661 kb Xq28 microduplication identified by chromosomal microarray analysis and confirmed also on DNA from muscle biopsy with a custom-designed X-chromosome-specific microarray. X-inactivation analysis revealed a skewed inactivation pattern on the proband's muscle biopsy. Read More

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December 2018

Identification of molecular signatures of cystic fibrosis disease status using plasma-based functional genomics

Physiol Genomics 2018 12 12. Epub 2018 Dec 12.

Department of Pediatrics, Medical College of Wisconsin, United States.

Although cystic fibrosis (CF) is attributed to dysfunction of a single gene, the relationships between the abnormal gene product and development of inflammation and progression of lung disease are not fully understood, limiting our ability to predict an individual patient's clinical course and treatment response. To better understand CF progression, we characterized the molecular signatures of CF disease status using plasma-based functional genomics. Peripheral blood mononuclear cells (PBMCs) from healthy donors were cultured with plasma samples from CF patients (n=103) and unrelated, healthy controls (n=31). Read More

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December 2018
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Plasminogen Activator Inhibitor 1 as a Poor Prognostic Indicator in Resectable Pancreatic Ductal Adenocarcinoma.

Chin Med J (Engl) 2018 Dec;131(24):2947-2952

Department of General Surgery, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100730, China.

Background: Plasminogen activator inhibitor 1 (PAI-1) was previously established to impact several phenotypes in many kinds of cancer, including pancreatic cancer. However, its prognostic significance in pancreatic ductal adenocarcinoma (PDAC) needs support of further evidence. This study was designed to address the issue. Read More

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December 2018
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miR-27a is highly expressed in H1650 cancer stem cells and regulates proliferation, migration, and invasion.

J Cancer Res Ther 2018 Dec;14(Supplement):S1004-S1011

Key Laboratory of Nutrition and Metabolism, Institute for Nutritional Sciences, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, University of Chinese Academy of Sciences, Shanghai, China.

Background: Cancer stem cells (CSCs) are responsible for tumor relapse after chemotherapy and radiotherapy in non-small cell lung cancer (NSCLC). The aim of this study is to explore the profile and role of microRNA (miRNA) in CSC of NSCLC.

Materials And Methods: We studied the expression of stem cell marker in side population cells and serum-free cultured spheres of NSCLC. Read More

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December 2018

Identification of disrupted pathways associated with colon cancer based on combining protein-protein interactions and pathway data.

J Cancer Res Ther 2018 Dec;14(Supplement):S998-S1003

Department of General Surgery, Xiangya Hospital, Central South University, Changsha, Hunan, P.R. China.

Objective: The objective of this paper was to identify the disrupted pathways associated with colon cancer at a network level based on protein-protein interaction (PPI) network and pathway analysis.

Materials And Methods: First of all, the Affymetrix microarray data of colon cancer, human PPIs relationships, and human pathways existed in the database were recruited and preprocessed. Second, differentially expressed genes (DEGs) between colon cancer and normal controls were identified. Read More

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December 2018

Unravelling reno-protective effects of SGLT2 inhibition in human proximal tubular cells.

Am J Physiol Renal Physiol 2018 Dec 12. Epub 2018 Dec 12.

Department of Internal Medicine IV, Nephrology and Hypertension, Medical University Innsbruck, Austria.

Large clinical trials demonstrated that SGLT2 inhibitors (SGLT2i) slow the progression of kidney function decline in type 2 diabetes. As the underlying molecular mechanisms are largely unknown, we studied the effects of SGLT2i on gene expression in two human proximal tubular (PT) cell lines under normoglycemic conditions, utilizing two SGLT2i, namely empagliflocin and canagliflocin. Genome-wide expression analysis did not reveal substantial differences between these two SGLT2i. Read More

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December 2018

Determination of Autoantibodies to Salivary Gland Antigens.

Methods Mol Biol 2019 ;1901:103-112

UCLA School of Dentistry, Los Angeles, CA, USA.

Primary Sjögren's syndrome (pSS) is a systemic autoimmune disease marked by dry mouth/dry eye symptoms as a result of the destruction of the salivary and lacrimal glands. Patients with pSS may be at risk for the development of mucosa-associated lymphoid tissue (MALT) lymphoma. Testing of autoantibodies in the oral fluid of patients with pSS or MALT lymphoma using immunoassays may lead to a simple and noninvasive clinical tool for diagnostic or prognostic applications. Read More

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January 2019

An Overview of DNA Analytical Methods.

Methods Mol Biol 2019 ;1897:385-402

Department of Pathology and Laboratory Medicine, David Geffen School of Medicine at UCLA, Los Angeles, CA, USA.

The development of rapid parallel sequencing in the last 20 years has begun a revolution in the field of genetics that is changing nearly all disciplines within biology and medicine. Genomic sequencing has become crucial to the diagnosis and clinical management of patients with constitutional diseases and cancer and has quickly become an integral part of the new era of personalized and precision medicine. The precision medicine initiative, released by the NIH in 2015, has catapulted genomic technologies to the forefront of the practice of medicine and biomedical research. Read More

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January 2019

Fundamentals of RNA Analysis on Biobanked Specimens.

Authors:
Samuel P Strom

Methods Mol Biol 2019 ;1897:345-357

Fulgent Genetics, Temple City, CA, USA.

Compared to DNA, analysis of RNA is one step closer on the central dogma of biology to assessing cellular function. This makes it an extremely valuable target for research and clinical testing in nearly all areas of molecular biology. Most RNA molecules are ephemeral by nature. Read More

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January 2019

Making a Tissue Microarray.

Methods Mol Biol 2019 ;1897:313-323

Department of Pathology and Laboratory Medicine, David Geffen School of Medicine at UCLA, Los Angeles, CA, USA.

Tissue microarray (TMA) is a widely used, high-throughput, cost-effective, and tissue and reagent-conserving method of performing molecular analysis. Multiple donor tissue cores are procured and transferred into a recipient TMA block for simultaneous differential and comparative molecular profiling under theoretically identical performance conditions. Described herein is a discussion of the theory behind the TMA, an overview of the concepts and principles of TMA design and construction, a brief summary of its advantages and disadvantages, and a sample protocol of TMA construction. Read More

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January 2019

Circulating MicroRNA-4739 May Be a Potential Biomarker of Critical Limb Ischemia in Patients with Diabetes.

Biomed Res Int 2018 14;2018:4232794. Epub 2018 Nov 14.

Department of Pharmacy, The Central Hospital of Wuhan, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430021, Hubei, China.

Critical limb ischemia (CLI) is the most severe manifestation of peripheral artery disease, which is common but rarely diagnosed. Noninvasive biomarkers are urgently required to assist in the diagnosis of CLI. Accumulating evidence indicates that miRNAs play an important role in the development of various diseases. Read More

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November 2018

Alteration of gene expression profile in CD3 T-cells after downregulating MALT1.

Immunotargets Ther 2018 20;7:77-81. Epub 2018 Nov 20.

Key Laboratory for Regenerative Medicine of Ministry of Education, Jinan University, Guangzhou, 510632, People's Republic of China,

Background: T cell immunodeficiency is a common feature in patients with different kinds of hematological disease such as T cell non-Hodgkin lymphoma (T-NHL), B cells NHL (B-NHL), NK/T cell NHL (NK/T-CL) and acute myeloid leukemia (AML). In our recent research, we found that significantly lower expression levels in MALT1 and NF-κB were related to suppression of T cell activation. Therefore, this study was conducted to further investigate the role of downregulating MALT1 in the development of immunodeficiency in T cells. Read More

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November 2018
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15q23 Gain in a Neonate with a Giant Omphalocele and Multiple Co-Occurring Anomalies.

Case Rep Pediatr 2018 13;2018:8702568. Epub 2018 Nov 13.

The Department of Pathology and Immunology, Washington University School of Medicine, Saint Louis, MO 63110, USA.

Background: Omphalocele is a rare congenital abdominal wall defect. It is frequently associated with genetic abnormality and other congenital anomalies, although isolated omphalocele cases do exist. Data have shown that omphalocele with co-occurring genetic abnormality has worse prognosis than isolated omphalocele. Read More

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November 2018

Potential Root Foraging Strategy of Wheat ( L.) for Potassium Heterogeneity.

Front Plant Sci 2018 27;9:1755. Epub 2018 Nov 27.

State Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science, Chinese Academy of Sciences, Nanjing, China.

Potassium (K) distribution is horizontally heterogeneous under the conservation agriculture approach of no-till with strip fertilization. The root foraging strategy of wheat for K heterogeneity is poorly understood. In this study, WinRHIZO, microarray, Non-invasive Micro-test Technology (NMT) and a split-root system were performed to investigate root morphology, gene expression profiling and fluxes of K and O under K heterogeneity and homogeneity conditions. Read More

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November 2018

Altered epidermal fatty acid-binding protein expression in hepatocellular carcinoma predicts unfavorable outcomes.

Cancer Manag Res 2018 23;10:6275-6284. Epub 2018 Nov 23.

Sun Yat-sen University Cancer Center, State Key Laboratory of Oncology in South China, Collaborative Innovation Center for Cancer Medicine, Guangzhou 510060, China,

Objective: Hepatocellular carcinoma (HCC) is a rapidly proliferating malignancy that requires large amounts of fatty acids to synthesize cellular membranes and provide energy. Epidermal fatty acid-binding protein (EFABP) is uniquely expressed in epidermal cells, but its role and expression in HCC are not clear.

Subjects And Methods: A total of 804 HCC specimens were collected to construct a tissue microarray (TMA) and for immunohistochemistry (IHC) analysis. Read More

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November 2018

Upregulation of KIF20A correlates with poor prognosis in gastric cancer.

Cancer Manag Res 2018 23;10:6205-6216. Epub 2018 Nov 23.

Department of General Surgery, The First Affiliated Hospital of Anhui Medical University, Hefei, Anhui, China,

Background: KIF20A is well known as one of the key proteins in mitosis. Recently, a number of studies illustrated that might function as an oncogene in some carcinomas. However, its expression levels and clinical value remained unclear in gastric cancer (GC). Read More

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November 2018

High mobility group box 3 as an emerging biomarker in diagnosis and prognosis of hepatocellular carcinoma.

Cancer Manag Res 2018 20;10:5979-5989. Epub 2018 Nov 20.

Medical School of Nantong University, Nantong 226001, Jiangsu, China,

Purpose: High mobility group box 3 (HMGB3) is associated with hepatocytes malignant transformation by our previous work. We continued to investigate the diagnostic and prognostic values of HMGB3 for hepatocellular carcinoma (HCC).

Patients And Methods: Circulating HMGB3 levels were quantitatively detected in a cohort of 225 patients with chronic liver diseases by ELISA and compared with alpha-fetoprotein by the receiver operating characteristic curve. Read More

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November 2018
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A predictive algorithm to identify genes that discriminate individuals with fibromyalgia syndrome diagnosis from healthy controls.

J Pain Res 2018 21;11:2981-2990. Epub 2018 Nov 21.

Symptoms Biology Unit, National Institute of Nursing Research (NINR), Bethesda, MD, USA.

Objectives: Fibromyalgia syndrome (FMS) is a chronic and often debilitating condition that is characterized by persistent fatigue, pain, bowel abnormalities, and sleep disturbances. Currently, there are no definitive prognostic or diagnostic biomarkers for FMS. This study attempted to utilize a novel predictive algorithm to identify a group of genes whose differential expression discriminated individuals with FMS diagnosis from healthy controls. Read More

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November 2018

Biomimetic intrafibrillar mineralized collagen promotes bone regeneration via activation of the Wnt signaling pathway.

Int J Nanomedicine 2018 21;13:7503-7516. Epub 2018 Nov 21.

Department of Oral & Maxillofacial-Head & Neck Oncology, Shanghai Ninth People's Hospital, Shanghai Jiaotong University School of Medicine, Shanghai Key Laboratory of Stomatology & Shanghai Research, Institute of Stomatology and National Clinical Research Center of Stomatology, Shanghai, China,

Purpose: The purpose of this study was to assess the effects of biomimetic intrafibrillar mineralized collagen (IMC) bone scaffold materials on bone regeneration and the underlying biological mechanisms.

Materials And Methods: A critical-sized bone defect in the rat femur was created; then IMC, extrafibrillar mineralized collagen, and nano-hydroxyapatite bone scaffold materials were grafted into the defect. Ten weeks after implantation, micro-computed tomography and histology were applied to evaluate the bone regeneration. Read More

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November 2018

Role of miRNAs in skeletal muscle aging.

Clin Interv Aging 2018 22;13:2407-2419. Epub 2018 Nov 22.

Department of Geriatrics, The First Hospital of Jilin University, Changchun 130021, Jilin, China,

Purpose: The aim of this study was to explore the role of miRNAs in the process of skeletal muscle aging.

Materials And Methods: We analyzed the miRNA microarray datasets from 19 young and 17 old skeletal muscle samples by bioinformatic analysis. Differentially expressed miRNAs were identified, followed by function and pathway enrichment analysis. Read More

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November 2018

The COX2 effector microsomal PGE2 synthase-1 is a regulator of immunosuppression in cutaneous melanoma.

Clin Cancer Res 2018 Dec 11. Epub 2018 Dec 11.

Department of Melanoma Medical Oncology, University of Texas MD Anderson Cancer Center

Purpose: Microsomal prostaglandin E2 synthase 1 (mPGES1) was evaluated as an important downstream effector of the cyclooxygenase 2 (COX2) pathway responsible for tumor-mediated immunosuppression in melanoma.

Experimental Design: The analysis of a Stage III melanoma tissue microarray (n=91) was performed to assess the association between mPGES1, COX2, CD8, and patient survival. Pharmacological inhibitors and syngeneic mouse models using mPGES1 knockout mouse melanoma cell lines were used to evaluate the mPGES1-mediated immunosuppressive function. Read More

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December 2018

Characterization of the Esi3/RCI2/PMP3 gene family in the Triticeae.

BMC Genomics 2018 Dec 11;19(1):898. Epub 2018 Dec 11.

Biology Department, Concordia University, 7141, Sherbrooke, W. Montreal (Quebec), H4B 1R6, Canada.

Background: Members of the Early Salt Induced 3 (Esi3/RCI2/PMP3) gene family in plants have been shown to be induced in response to both biotic and abiotic stresses and to enhance stress tolerance in both transgenic plants and Saccharomyces cerevisiae. Esi3 was first identified as a salt stress induced gene in the salt tolerant wild wheat grass, Lophopyrum elongatum, and subsequently homologous genes in many other species were found to be members of the gene family. These include Arabidopsis thaliana and Oryza sativa where they are referred to as Rare Cold Inducible 2 (RCI2), and Zea mays where they are referred to as Plasma Membrane Protein 3 (PMP3). Read More

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December 2018
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Effects of long-term physical exercise on skeletal muscles in senescence-accelerated mice (SAMP8).

Biosci Biotechnol Biochem 2018 Dec 12:1-7. Epub 2018 Dec 12.

a Graduate School of Life and Environmental Sciences , Kyoto Prefectural University , Kyoto , Japan.

We examined the effect of long-term exercise on the prevention of sarcopenia using a senescence-accelerated-prone mice (SAMP8) model. Mice were housed in a wheel cage for 25 weeks to increase voluntary exercise. At week 23, endurance running capacity was examined using a treadmill. Read More

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December 2018

Stromal Cell-Derived CCL20 Promotes Tumor Progression and Osteolysis in Giant Cell Tumor of Bone.

Cell Physiol Biochem 2018 11;51(5):2472-2483. Epub 2018 Dec 11.

Spine Tumor Center, Department of Orthopedic Oncology, Changzheng Hospital, Second Military Medical University, Shanghai, China.

Background/aims: Giant cell tumor of bone (GCTB), one of the most common primary bone tumors, leads to extensive bone destruction. However, the mechanisms underlying GCTB progression remain elusive and prognostic factors and treatment targets are required. In the current study, we explored the function of the chemokine family member CCL20 in GCTB progression. Read More

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December 2018
3 Reads

MicroRNA-128 Protects Dopamine Neurons from Apoptosis and Upregulates the Expression of Excitatory Amino Acid Transporter 4 in Parkinson's Disease by Binding to AXIN1.

Cell Physiol Biochem 2018 7;51(5):2275-2289. Epub 2018 Dec 7.

Department of Neurosurgery, The First Affiliated Hospital of Kunming Medical University, Kunming,

Background/aims: Parkinson's disease (PD) is a frequently occurring condition that resulted from the loss of midbrain neurons, which synthesize the neurotransmitter dopamine. In this study, we established mouse models of PD to investigate the expression of microRNA-128 (miR-128) and mechanism through which it affects apoptosis of dopamine (DA) neurons and the expression of excitatory amino acid transporter 4 (EAAT4) via binding to axis inhibition protein 1 (AXIN1).

Methods: Gene expression microarray analysis was performed to screen differentially expressed miRNAs that are associated with PD. Read More

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December 2018
2 Reads

circZMYM2 Competed Endogenously with miR-335-5p to Regulate JMJD2C in Pancreatic Cancer.

Cell Physiol Biochem 2018 7;51(5):2224-2236. Epub 2018 Dec 7.

Departments of Urology, the Third Affiliated Hospital of SooChow University, Changzhou,

Background/aims: We aimed to study the involvement of circZMYM2 (hsa_circ_0099999) in pancreatic cancer (PC) cell proliferation, apoptosis and invasion and to figured out the underlying mechanism of circZMYM2 regulating miR-335-5p and JMJD2C.

Methods: CircRNA differential expressions in twenty PC samples and paired normal tissue samples were analyzed using Arraystar Human CircRNA microarray V1. CircZMYM2 expression level was determined via qRT-PCR. Read More

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December 2018
1 Read

Molecular Evolution of Early-Onset Prostate Cancer Identifies Molecular Risk Markers and Clinical Trajectories.

Cancer Cell 2018 Dec;34(6):996-1011.e8

Finsen Laboratory, Rigshospitalet, DK-2200, Copenhagen, Denmark; Biotech Research & Innovation Centre (BRIC), University of Copenhagen, DK-2200, Copenhagen, Denmark; European Molecular Biology Laboratory (EMBL), Genome Biology Unit, 69120 Heidelberg, Germany; Charité Universitätsmedizin Berlin, Charitéplatz 1, D-10117 Berlin, Germany. Electronic address:

Identifying the earliest somatic changes in prostate cancer can give important insights into tumor evolution and aids in stratifying high- from low-risk disease. We integrated whole genome, transcriptome and methylome analysis of early-onset prostate cancers (diagnosis ≤55 years). Characterization across 292 prostate cancer genomes revealed age-related genomic alterations and a clock-like enzymatic-driven mutational process contributing to the earliest mutations in prostate cancer patients. Read More

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December 2018
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Clinicopathologic and prognostic significance of MKK4 and MKK7 in resectable pancreatic ductal adenocarcinoma.

Hum Pathol 2018 Dec 8. Epub 2018 Dec 8.

Department of General Surgery, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences/Peking Union Medical College, Beijing 100730, China. Electronic address:

Mitogen-activated protein kinase kinase 4 (MKK4) and mitogen-activated protein kinase kinase 7 (MKK7) were shown to regulate biological behavior in many malignancies. In pancreatic ductal adenocarcinoma (PDAC), it remains controversial whether MKK4 and MKK7 have pro-oncogenic or tumor-suppressive activities. Furthermore, their clinicopathologic and prognostic implications are unknown. Read More

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December 2018

Feature selection and tumor classification for microarray data using relaxed Lasso and generalized multi-class support vector machine.

J Theor Biol 2018 Dec 8. Epub 2018 Dec 8.

College of Mathematics and Physics, Qingdao University of Science and Technology, Qingdao 266061, China; Artificial Intelligence and Biomedical Big Data Research Center, Qingdao University of Science and Technology, Qingdao 266061, China; School of Life Sciences, University of Science and Technology of China, Hefei 230027, China. Electronic address:

At present, the study of gene expression data provides a reference for tumor diagnosis at the molecular level. It is a challenging task to select the feature genes related to the classification from the high-dimensional and small-sample gene expression data and successfully separate the different subtypes of tumor or the normal from the patient. In this paper, we present a new method for tumor classification-relaxed Lasso (least absolute shrinkage and selection operator) and generalized multi-class support vector machine (rL-GenSVM). Read More

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December 2018

Follicle Populations and Gene Expression Profiles of Nelore and Angus Heifers with Low and High Ovarian Follicle Counts.

Mol Reprod Dev 2018 Dec 8. Epub 2018 Dec 8.

Department of Pharmacology, , Institute of Biosciences, São Paulo State University (UNESP), Botucatu, Brazil.

The number of antral follicles counted by ultrasound (AFC) is associated with fertility in cattle. Cows with higher follicle count (HFC) have higher performance in reproductive-assisted technologies than cows with lower follicle count (LFC). In this study, we aimed to define the preantral follicle count by histology and to identify differentially expressed genes (DEGs) using a microarray in Nelore and Angus heifers with HFC and LFC. Read More

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December 2018

MYD88 L265P mutations identify a prognostic gene expression signature and a pathway for targeted inhibition in CLL.

Br J Haematol 2018 Dec 9. Epub 2018 Dec 9.

Department of Medical Oncology, Dana-Farber Cancer Institute, Cambridge, MA, USA.

The L265P somatic mutation in the Myeloid Differentiation Primary Response 88 (MYD88) gene is a recurrent mutation in chronic lymphocytic leukaemia (CLL). This mutation has functional effects in various haematological malignancies but its role in CLL remains to be fully elucidated. Here, we report that MYD88 L265P mutations are associated with mutated immunoglobulin heavy-chain gene (IGHV-M) status and that among IGHV-M patients, the presence of MYD88 L265P is associated with younger age at diagnosis. Read More

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December 2018

Combination of Whole Grape Seed Flour and Newly Isolated Kefir Lactic Acid Bacteria Reduces High-Fat (HF)-induced Hepatic Steatosis.

Mol Nutr Food Res 2018 Dec 11:e1801040. Epub 2018 Dec 11.

J.-H. Kwon, H. G. Lee, K.-H. Seo, H. Kim, Department of Food and Nutrition, Hanyang University, Seoul.

Scope: This study aimed to determine the separate and combined effect of a prebiotic (Catechin-rich wine grape seed flour, GSF) and a probiotic (newly isolated kefir lactic acid bacteria, LAB) on hepatic steatosis of obese mice.

Methods And Results: High-fat (HF)-induced obese mice were fed HF diet containing 6% microcrystalline cellulose (MCC, control), 10% GSF, orally administrated LAB, or combination of GSF and LAB for 9 weeks. There was a significant reduction of body weight gain, and liver weights, plasma insulin concentrations, and HOMA-IR in all experimental groups compared to control. Read More

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December 2018

miR-125a/b inhibits tumor-associated macrophages mediated in cancer stem cells of hepatocellular carcinoma by targeting CD90.

J Cell Biochem 2018 Dec 9. Epub 2018 Dec 9.

Department of General Surgery, Shanghai Tongji Hospital, Medical School of Tongji University, Shanghai, China.

Cancer stem cells promote tumorigenesis and progression of hepatocellular carcinoma (HCC). Recently, emerging evidence indicates tumor-associated macrophages (TAMs) play an important role in tumor progression. However, TAMs often occurs with unknown mechanisms. Read More

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December 2018

IRAK3 gene silencing prevents cardiac rupture and ventricular remodeling through negative regulation of the NF-κB signaling pathway in a mouse model of acute myocardial infarction.

J Cell Physiol 2018 Dec 7. Epub 2018 Dec 7.

Department of Cardiovascular Surgery, Henan Provincial People's Hospital, Zhengzhou, P. R. China.

Cardiac rupture and ventricular remodeling are recognized as the severe complications and major risk factors of acute myocardial infarction (AMI). This study aims to evaluate the regulatory roles of interleukin-1 receptor-associated kinase 3 (IRAK3) and nuclear factor-κB (NF-κB) signaling pathway in cardiac rupture and ventricular remodeling. Microarray analysis was performed to screen AMI-related differentially expressed genes and IRAK3 was identified. Read More

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December 2018

Differential Mucosal Gene Expression Patterns in Candida-Associated, Chronic Oral Denture Stomatitis.

J Prosthodont 2018 Dec 10. Epub 2018 Dec 10.

Department of Pharmaceutical and Biomedical Sciences, Touro College of Pharmacy, New York, NY.

Purpose: Denture stomatitis is a common condition manifested by inflammation of the oral mucous membrane beneath a denture. The objective of this study was to compare the transcriptome of human palatal mucosa with chronic oral stomatitis-associated Candida albicans infection to that of healthy oral mucosa.

Materials And Methods: Oral palatal biopsies were obtained from 17 healthy and 15 C. Read More

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December 2018

Intellectual, adaptive and behavioural characteristics in four patients with 18p deletion syndrome.

J Intellect Disabil Res 2018 Dec 9. Epub 2018 Dec 9.

Genetics Division, Department of Morphology and Genetics, Universidade Federal de São Paulo, São Paulo, Brazil.

Background: The association of behavioural phenotype assessment with cytogenomic characterisation may provide a better comprehension of genotype-phenotype correlations in syndromes caused by chromosomal abnormalities, such as 18p deletion syndrome.

Method: We report on four Brazilian patients with 18p deletion syndrome characterised by cytogenomic techniques and detailed neuropsychological evaluation. Intellectual, adaptive and behavioural characteristics were assessed through the Wechsler's Scales, the Vineland-II Scale and the Child Behaviour Checklist, respectively. Read More

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December 2018
1 Read

Toll-like receptor 2 regulates metabolic reprogramming in gastric cancer via superoxide dismutase 2.

Int J Cancer 2018 Dec 11. Epub 2018 Dec 11.

Department of General Surgery, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, 200080, China.

Toll-like receptors (TLRs) play critical roles in host defence following recognition of conserved microbial- and host-derived components, and their dysregulation is a common feature of various inflammation-associated cancers, including gastric cancer (GC). Despite the recent recognition that metabolic reprogramming is a hallmark of cancer, the molecular effectors of altered metabolism during tumorigenesis remain unclear. Here, using bioenergetics function assays on human GC cells, we reveal that ligand-induced activation of TLR2, predominantly through TLR1/2 heterodimer, augments both oxidative phosphorylation (OXPHOS) and glycolysis, with a bias towards glycolytic activity. Read More

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December 2018
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The P-class pentatricopeptide repeat protein PpPPR_21 is needed for accumulation of the psbI-ycf12 dicistronic mRNA in Physcomitrella chloroplasts.

Plant J 2018 Dec 7. Epub 2018 Dec 7.

Center for Gene Research, Nagoya University, Nagoya, 464-8602, Japan.

Chloroplast gene expression is controlled by numerous nuclear-encoded RNA-binding proteins. Among them, pentatricopeptide repeat (PPR) proteins are known to be key players of posttranscriptional regulation in chloroplasts. However, the functions of many PPR proteins remain unknown. Read More

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December 2018

Prostate cancer induces C/EBPβ expression in surrounding epithelial cells which relates to tumor aggressiveness and patient outcome.

Prostate 2018 Dec 11. Epub 2018 Dec 11.

Department of Medical Biosciences, Pathology, Umeå University, Umeå, Sweden.

Background: Implantation of rat prostate cancer cells into the normal rat prostate results in tumor-stimulating adaptations in the tumor-bearing organ. Similar changes are seen in prostate cancer patients and they are related to outcome. One gene previously found to be upregulated in the non-malignant part of tumor-bearing prostate lobe in rats was the transcription factor CCAAT/enhancer-binding protein-β (C/EBPβ). Read More

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December 2018
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MiR-15b-5p is Involved in Doxorubicin-Induced Cardiotoxicity via Inhibiting Bmpr1a Signal in H9c2 Cardiomyocyte.

Cardiovasc Toxicol 2018 Dec 7. Epub 2018 Dec 7.

Department of Cardiology, The Second Affiliated Hospital of Shantou University Medical College, Dongxia North Road, Shantou, 515041, Guangdong, People's Republic of China.

The wide use of anthracyclines represented by doxorubicin (DOX) has benefited cancer patients, yet the clinical application is limited due to its cardiotoxicity. Although numerous evidences have supported a role of microRNAs (miRNAs) in DOX-induced myocardial damage, the exact etiology and pathogenesis remain largely obscure. In this study, we focused on the role of miR-15b-5p in DOX-induced cardiotoxicity. Read More

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December 2018

Expression profile of long non-coding RNAs in rat models of OSA-induced cardiovascular disease: new insight into pathogenesis.

Sleep Breath 2018 Dec 7. Epub 2018 Dec 7.

Department of Respiratory Disease, The First Affiliated Hospital of Fujian Medical University, No.20 Chazhong Road, Fuzhou, 350005, Taijiang District, China.

Purpose: Long non-coding RNAs (lncRNAs) are a recently identified class of regulatory molecules involved in the regulation of numerous biological processes, but their functions in a rat model of chronic intermittent hypoxia (CIH) remain largely unknown. Therefore, for further investigation, we aimed to explore lncRNA expression profiles and reveal their potential functional roles in rat models of CIH.

Methods: We used a well-established CIH rat model and conducted lncRNA microarray experiments on the heart samples of rats with CIH and under normoxia control. Read More

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December 2018

Expression levels and co‑targets of miRNA‑126‑3p and miRNA‑126‑5p in lung adenocarcinoma tissues: Αn exploration with RT‑qPCR, microarray and bioinformatic analyses.

Oncol Rep 2018 Dec 4. Epub 2018 Dec 4.

Department of Pathology, First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi Zhuang Autonomous Region 530021, P.R. China.

Lung adenocarcinoma (LUAD) is the most common histological subtype of lung cancer. Previous studies have found that many microRNAs (miRNAs), including miRNA‑126‑3p, may play a critical role in the development of LUAD. However, no study of LUAD has researched the synergistic effects and co‑targets of both miRNA‑126‑3p and miRNA‑126‑5p. Read More

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December 2018

MicroRNA‑143‑3p suppresses cell growth and invasion in laryngeal squamous cell carcinoma via targeting the k‑Ras/Raf/MEK/ERK signaling pathway.

Authors:
Feng Zhang Hua Cao

Int J Oncol 2018 Dec 6. Epub 2018 Dec 6.

Ear Nose and Throat Hospital, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan 450052, P.R. China.

MicroRNAs (miRNAs or miRs) have been identified as an important regulator in carcinogenesis and other pathological processes. However, the molecular mechanism underlying the function of miRNAs in the progression and development of laryngeal squamous cell carcinoma (LSCC) remains to be fully elucidated. In the present study, the miRNA expression pattern in LSCC tissues was profiled using miRNA microarray analysis. Read More

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December 2018

miR‑138‑5p modulates the expression of excision repair cross‑complementing proteins ERCC1 and ERCC4, and regulates the sensitivity of gastric cancer cells to cisplatin.

Oncol Rep 2018 Dec 6. Epub 2018 Dec 6.

Department of Oncology, The First Affiliated Hospital of Anhui Medical University, Hefei, Anhui 230032, P.R. China.

The microRNA (miR)‑138‑5p affects the chemotherapeutic sensitivity of several human cancer types. In the present study, the expression and regulatory mechanisms of miR‑138‑5p were investigated in the gastric cancer cell line SGC7901 and its cisplatin‑resistant derivative SGC7901/DDP. Gene microarray and reverse transcription‑quantitative polymerase chain reaction analyses revealed that miR‑138‑5p was expressed at significantly lower levels in SGC7901/DDP compared with SGC7901 cells. Read More

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December 2018

Inactivation of RUNX3 protein expression in tongue squamous cell carcinoma and its association with clinicopathological characteristics.

Mol Med Rep 2018 Nov 29. Epub 2018 Nov 29.

Jiangsu Center for The Collaboration and Innovation of Cancer Biotherapy, Cancer Institute, Xuzhou Medical University, Xuzhou, Jiangsu 221002, P.R. China.

The function of runt‑related transcription factor 3 (RUNX3) in oral cancer remains controversial. The present study aimed to determine the status of RUNX3 protein expression and its association with clinicopathological characteristics in tongue squamous cell carcinomas (SCC). The present study used three pairs of tongue SCC and non‑cancerous tissues to assess RUNX3 protein expression by western blot analysis, and two tongue SCC cell lines to determine RUNX3 protein localization by immunofluorescence and immunocytochemistry. Read More

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November 2018

MicroRNA‑100‑5p inhibits osteoclastogenesis and bone resorption by regulating fibroblast growth factor 21.

Int J Mol Med 2018 Dec 6. Epub 2018 Dec 6.

Department of Orthopedic Surgery, Shengjing Hospital of China Medical University, Shenyang, Liaoning 110004, P.R. China.

MicroRNAs (miRNAs/miRs) are post‑transcriptional regulators that serve important roles in osteoclastogenesis and bone metabolism; however, the roles of miRNAs have not been completely clarified. The present study aimed to investigate the effects of miR‑100‑5p on the mechanism of liver‑bone endocrine metabolism. A miRNA microarray analysis was conducted to evaluate the miRNA expression profile during receptor activator of nuclear factor‑κB ligand‑stimulated osteoclastogenesis. Read More

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December 2018

Effects of Icariin on Atherosclerosis and Predicted Function Regulatory Network in ApoE Deficient Mice.

Biomed Res Int 2018 6;2018:9424186. Epub 2018 Nov 6.

Department of Pharmacology and Toxicology, Jilin University School of Pharmaceutical Sciences, 1163 Xinmin Ave, Changchun, 130021, China.

Icariin plays a pivotal role in ameliorating atherosclerosis for animal models although its comprehensive biological role remains largely unexplored. This study aimed to fully understand the effects of icariin on atherosclerosis in high-fat diet-induced ApoE mice and investigate mRNA-miRNA regulation based on microarray and bioinformatics analysis. The areas of atherosclerotic lesions in en face aorta were evaluated. Read More

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November 2018

Pancreatic-cancer-cell-derived trefoil factor 2 impairs maturation and migration of human monocyte-derived dendritic cells .

Anim Cells Syst (Seoul) 2018 1;22(6):368-381. Epub 2018 Oct 1.

Institute for Healthcare and Life Science and Institute for Translational and Clinical Research, Catholic Kwandong University International St. Mary's Hospital, Incheon, Republic of Korea.

Pancreatic cancer is a challenging disease with a high mortality rate. While the importance of crosstalk between cancer and immune cells has been well documented, the understanding of this complex molecular network is incomplete. Thus, identification of the secreted proteins contributing to the immunosuppressive microenvironment in pancreatic cancer is crucial for effective diagnosis and/or therapy. Read More

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October 2018

Validation of epigenetic mechanisms regulating gene expression in canine B-cell lymphoma: An in vitro and in vivo approach.

PLoS One 2018 11;13(12):e0208709. Epub 2018 Dec 11.

Department of Comparative Biomedicine and Food Science, University of Padova, Padova, Italy.

Despite canine B-cell Lymphoma (BCL) representing the most common haematological tumour, epigenetic events driving development and progression are scarcely known. Recently, canine Diffuse Large BCL (DLBCL) DNA methylome by genome-wide CpG microarray has identified genes and pathways associated to pathogenesis. To validate data previously obtained by array analysis, the CLBL-1 cell line was used and the HOXD10, FGFR2, ITIH5 and RASAL3 genes were selected. Read More

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December 2018

Enolase-1 serves as a biomarker of diagnosis and prognosis in hepatocellular carcinoma patients.

Cancer Manag Res 2018 15;10:5735-5745. Epub 2018 Nov 15.

Precision Medicine Center, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan 450052, China,

Background: Hepatocellular carcinoma (HCC) is an aggressive malignancy with high incidence rate and poor prognosis. Enolase-1 (ENO1), a key glycolytic enzyme, has been implicated in the tumorigenesis of various cancers. However, its diagnostic value and clinical significance in HCC are unclear. Read More

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November 2018
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