58 results match your criteria Microarray Technologies in the Diagnosis and Treatment of Head and Neck Cancer


Application of Tissue Microarray in Esophageal Adenocarcinoma.

Methods Mol Biol 2018 ;1756:105-118

Cancer Molecular Pathology of School of Medicine, Griffith University, Gold Coast, Australia.

Tissue microarray technology could allow immunohistochemical staining or in situ hybridization on hundreds of different tissue samples simultaneously. It allows faster analysis and considerably reducing costs incurred in staining. The technique also provides a high-throughput analysis of multiple tissues for the different types of research. Read More

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http://dx.doi.org/10.1007/978-1-4939-7734-5_10DOI Listing
January 2019
1 Read

Identification of human papillomavirus (HPV) subtype in oral cancer patients through microarray technology.

Eur Arch Otorhinolaryngol 2018 Feb 9;275(2):535-543. Epub 2017 Dec 9.

Department of Oral Pathology, College of Dentistry, Gangneung-Wonju National University, Gangneung, South Korea.

Purpose: Human papilloma virus (HPV) is the main source of cervical cancer. Many recent studies have revealed the prevalence and prognosis of HPV associated with oropharyngeal squamous cell carcinoma, but fewer reports have evaluated HPV in oral squamous cell carcinoma (OSCC). The purpose of this study was to determine the prevalence and prognosis of HPV associated with OSCC according to HPV and tumor types. Read More

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http://link.springer.com/10.1007/s00405-017-4828-9
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http://dx.doi.org/10.1007/s00405-017-4828-9DOI Listing
February 2018
6 Reads

CXCR4/CXCR7/CXCL12 axis promotes an invasive phenotype in medullary thyroid carcinoma.

Br J Cancer 2017 Dec 7;117(12):1837-1845. Epub 2017 Nov 7.

Department of Surgery (A), Heinrich-Heine-University and University Hospital Duesseldorf, Moorenstr. 5, Duesseldorf 40225, Germany.

Background: Medullary thyroid carcinoma (MTC) is a rare and challenging endocrine malignancy. Once spread, the therapeutic options are limited and the outcome poor. For these patients, the identification of new druggable biological markers is of great importance. Read More

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http://dx.doi.org/10.1038/bjc.2017.364DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5729476PMC
December 2017
6 Reads

Toll-like receptor 5 and 7 expression may impact prognosis of HPV-positive oropharyngeal squamous cell carcinoma patients.

Cancer Immunol Immunother 2017 Dec 30;66(12):1619-1629. Epub 2017 Aug 30.

Pathology, University of Helsinki, HUSLAB, and Helsinki University Hospital, P.O Box 21, 00014 University of Helsinki, Helsinki, Finland.

A large subset of oropharyngeal squamous cell carcinomas (OPSCCs) is associated with HPV infection and has better outcome than non-viral-related tumors. Various malignancies also carry a role for TLRs, key activators of inflammation and innate immunity. We examined the expression of TLRs in OPSCC, and their association with HPV status and treatment outcome. Read More

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http://dx.doi.org/10.1007/s00262-017-2054-3DOI Listing
December 2017
3 Reads

Weighted gene co-expression network analysis of gene modules for the prognosis of esophageal cancer.

Authors:
Cong Zhang Qian Sun

J Huazhong Univ Sci Technolog Med Sci 2017 Jun 6;37(3):319-325. Epub 2017 Jun 6.

Cancer Biology Research Center & Key Laboratory of the Ministry of Education, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China.

Esophageal cancer is a common malignant tumor, whose pathogenesis and prognosis factors are not fully understood. This study aimed to discover the gene clusters that have similar functions and can be used to predict the prognosis of esophageal cancer. The matched microarray and RNA sequencing data of 185 patients with esophageal cancer were downloaded from The Cancer Genome Atlas (TCGA), and gene co-expression networks were built without distinguishing between squamous carcinoma and adenocarcinoma. Read More

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http://dx.doi.org/10.1007/s11596-017-1734-8DOI Listing
June 2017
4 Reads

Significance of PI3K/AKT signaling pathway in metastasis of esophageal squamous cell carcinoma and its potential as a target for anti-metastasis therapy.

Oncotarget 2017 Jun;8(24):38755-38766

School of Biomedical Sciences, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Pokfulam, Hong Kong SAR, China.

Metastasis is the most lethal hallmark of esophageal squamous cell carcinoma (ESCC). The aim of the study is to identify key signaling pathways that control metastasis in ESCC. Highly invasive ESCC sublines (designated I3 cells) were established through three rounds of selection of cancer cells invading through matrigel-coated chambers. Read More

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http://dx.doi.org/10.18632/oncotarget.16333DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5503569PMC
June 2017
16 Reads

An Expression Signature as an Aid to the Histologic Classification of Non-Small Cell Lung Cancer.

Clin Cancer Res 2016 Oct 28;22(19):4880-4889. Epub 2016 Jun 28.

Hamon Center for Therapeutic Oncology Research, University of Texas Southwestern Medical Center, Dallas, Texas. Simmons Cancer Center, University of Texas Southwestern Medical Center, Dallas, Texas. Department of Pathology, University of Texas Southwestern Medical Center, Dallas, Texas.

Purpose: Most non-small cell lung cancers (NSCLC) are now diagnosed from small specimens, and classification using standard pathology methods can be difficult. This is of clinical relevance as many therapy regimens and clinical trials are histology dependent. The purpose of this study was to develop an mRNA expression signature as an adjunct test for routine histopathologic classification of NSCLCs. Read More

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http://dx.doi.org/10.1158/1078-0432.CCR-15-2900DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5492382PMC
October 2016
35 Reads

PDZ binding kinase (PBK) is a theranostic target for nasopharyngeal carcinoma: driving tumor growth via ROS signaling and correlating with patient survival.

Oncotarget 2016 May;7(18):26604-16

State Key Laboratory of Oncology in South China, Collaborative Innovation Center for Cancer Medicine, Sun Yat-sen University Cancer Center, Guangzhou, Guangdong, China.

Nasopharyngeal carcinoma (NPC) is well known as one of the most common malignancies in southern China and Southeast Asia. However, the mechanisms underlying NPC progression remain poorly understood. Herein, through overlapping the differentially expressed genes from 3 microarray data sets with the human kinome, we identified PBK, a serine-threonine kinase, is highly upregulated and has not been intensively investigated in NPC. Read More

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5042002PMC
http://dx.doi.org/10.18632/oncotarget.8445DOI Listing
May 2016
7 Reads

Gene signature of the post-Chernobyl papillary thyroid cancer.

Eur J Nucl Med Mol Imaging 2016 Jul 26;43(7):1267-77. Epub 2016 Jan 26.

Department of Nuclear Medicine and Endocrine Oncology, Maria Skłodowska-Curie Memorial Cancer Center and Institute of Oncology, Gliwice Branch, Wybrzeze Armii Krajowej 15, 44-101, Gliwice, Poland.

Purpose: Following the nuclear accidents in Chernobyl and later in Fukushima, the nuclear community has been faced with important issues concerning how to search for and diagnose biological consequences of low-dose internal radiation contamination. Although after the Chernobyl accident an increase in childhood papillary thyroid cancer (PTC) was observed, it is still not clear whether the molecular biology of PTCs associated with low-dose radiation exposure differs from that of sporadic PTC.

Methods: We investigated tissue samples from 65 children/young adults with PTC using DNA microarray (Affymetrix, Human Genome U133 2. Read More

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http://dx.doi.org/10.1007/s00259-015-3303-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4869750PMC
July 2016
12 Reads

Meta-Analyses of Microarray Datasets Identifies ANO1 and FADD as Prognostic Markers of Head and Neck Cancer.

PLoS One 2016 25;11(1):e0147409. Epub 2016 Jan 25.

Integrated Head and Neck Oncology Program, Mazumdar Shaw Centre for Translational Research, Mazumdar Shaw Medical Centre, Narayana Health, Bangalore, Karnataka, India.

The head and neck squamous cell carcinoma (HNSCC) transcriptome has been profiled extensively, nevertheless, identifying biomarkers that are clinically relevant and thereby with translational benefit, has been a major challenge. The objective of this study was to use a meta-analysis based approach to catalog candidate biomarkers with high potential for clinical application in HNSCC. Data from publically available microarray series (N = 20) profiled using Agilent (4X44K G4112F) and Affymetrix (HGU133A, U133A_2, U133Plus 2) platforms was downloaded and analyzed in a platform/chip-specific manner (GeneSpring software v12. Read More

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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0147409PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4726811PMC
July 2016
18 Reads

Copy number increase of oncoprotein CIP2A is associated with poor patient survival in human head and neck squamous cell carcinoma.

J Oral Pathol Med 2016 May 5;45(5):329-37. Epub 2015 Oct 5.

Centre for Biotechnology, University of Turku and Åbo Akademi University, Turku, Finland.

Background: CIP2A, an inhibitor of PP2A tumour suppressor function, is a widely overexpressed biomarker of aggressive disease and poor therapy response in multiple human cancer types.

Methods: CIP2A and DPPA4 copy number alterations and expression were analysed by fluorescence in situ hybridisation (FISH) and immunohistochemistry (IHC) in different cell lines and a tissue microarray of 52 HNSCC patients. Results were correlated with patient survival and other clinicopathological data. Read More

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http://dx.doi.org/10.1111/jop.12372DOI Listing
May 2016
6 Reads

Developing Robust Predictive Models for Head and Neck Cancer across Microarray and RNA-seq Data.

ACM BCB 2015 Sep;2015:393-402

Department of Biomedical Engineering Georgia Institute of Technology Atlanta, GA 1-404-385-5059.

Increased understanding of the transcriptomic patterns underlying head and neck squamous cell carcinoma (HNSCC) can facilitate earlier diagnosis and better treatment outcomes. Integrating knowledge from multiple studies is necessary to identify fundamental, consistent gene expression signatures that distinguish HNSCC patient samples from disease-free samples, and particularly for detecting HNSCC at an early pathological stage. This study utilizes feature integration and heterogeneous ensemble modeling techniques to develop robust models for predicting HNSCC disease status in both microarray and RNAseq datasets. Read More

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http://dx.doi.org/10.1145/2808719.2808760DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5859557PMC
September 2015

The upregulated α-catulin expression was involved in head-neck squamous cell carcinogenesis by promoting proliferation, migration, invasion, and epithelial to mesenchymal transition.

Tumour Biol 2016 Feb 27;37(2):1671-81. Epub 2015 Aug 27.

Cancer Research Center, The Key Laboratory of Brain and Spinal Cord Injury of Liaoning Province, Laboratory Animal Center, The First Affiliated Hospital of Liaoning Medical University, Jinzhou, 121001, China.

Rho signaling component, α-catulin, is a cytoskeletal linker protein and plays an important role in apoptotic and senescence resistance, cytoskeletal reorganization, mobility, invasion, and epithelial to mesenchymal transition (EMT) of cancer cells. Here, we transfected α-catulin-expressing plasmid into head and neck squamous cell carcinoma (HNSCC) cell and examined the phenotypes and relevant molecules. α-catulin expression was detected on tissue microarray containing squamous epithelium, dysplasia, and cancer of head and neck by immunohistochemistry. Read More

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http://dx.doi.org/10.1007/s13277-015-3901-5DOI Listing
February 2016
41 Reads

Global transcriptome and sequenome analysis of formalin-fixed salivary epithelial-myoepithelial carcinoma specimens.

Genes Chromosomes Cancer 2015 Apr 29;54(4):249-59. Epub 2014 Dec 29.

Serviço de Anatomia Patológica, Instituto Português de Oncologia Francisco Gentil, Lisboa, Portugal & Instituto de Anatomia Patológica, Faculdade de Medicina de Lisboa, Portugal.

Diverse microarray and sequencing technologies have been widely used to characterize molecular changes in malignant epithelial cells in salivary neoplasms. Such gene expression studies to identify markers and targets in tumor cells are, however, compromised by the cellular heterogeneity of these tumors and by the difficulties to accrue matching controls representing normal salivary glands. Seventeen samples of primary salivary epithelial-myoepithelial carcinoma along with tissue from six normal major salivary glands were microdissected from paraffin-embedded tissue. Read More

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http://dx.doi.org/10.1002/gcc.22238DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4845898PMC
April 2015
6 Reads

[Idenfication of microRNAs profiles in nasopharyngeal carcinoma].

Lin Chung Er Bi Yan Hou Tou Jing Wai Ke Za Zhi 2014 Jul;28(13):975-8

Objective: To filtrate and prove the different microRNAs (miRs) profiles in nasopharyngeal carcinoma.

Method: Screening the different expressions of miRs between nasopharyngeal carcinoma and the inflammatory tissues by the application of expression profiling of chip high-throughput and large-scale microarray analysis. Then we used RT-QPCR technology to prove the accuracy of screening results. Read More

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July 2014
10 Reads

Mutation and Transcriptional Profiling of Formalin-Fixed Paraffin Embedded Specimens as Companion Methods to Immunohistochemistry for Determining Therapeutic Targets in Oropharyngeal Squamous Cell Carcinoma (OPSCC): A Pilot of Proof of Principle.

Head Neck Pathol 2015 Jun 19;9(2):223-35. Epub 2014 Sep 19.

Department of Otolaryngology and Head and Neck Oncology Program, Winship Cancer Institute of Emory University, Emory University School of Medicine, Atlanta, GA, USA,

The role of molecular methods in the diagnosis of head and neck cancer is rapidly evolving and holds great potential for improving outcomes for all patients who suffer from this diverse group of malignancies . However, there is considerable debate as to the best clinical approaches, particularly for Next Generation Sequencing (NGS). The choices of NGS methods such as whole exome, whole genome, whole transcriptomes (RNA-Seq) or multiple gene resequencing panels, each have strengths and weakness based on data quality, the size of the data, the turnaround time for data analysis, and clinical actionability. Read More

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http://link.springer.com/content/pdf/10.1007%2Fs12105-014-05
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http://link.springer.com/10.1007/s12105-014-0566-0
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http://dx.doi.org/10.1007/s12105-014-0566-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4424213PMC
June 2015
27 Reads

Development of TRAIL resistance by radiation-induced hypermethylation of DR4 CpG island in recurrent laryngeal squamous cell carcinoma.

Int J Radiat Oncol Biol Phys 2014 Apr;88(5):1203-11

Department of Otolaryngology, Asan Medical Center, University of Ulsan College of Medicine, Seoul, Republic of Korea; Biomedical Research Institute, Korea Institute of Science and Technology, Seoul, Republic of Korea. Electronic address:

Purpose: There are limited therapeutic options for patients with recurrent head and neck cancer after radiation therapy failure. To assess the use of tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) as a salvage chemotherapeutic agent for recurrent cancer after radiation failure, we investigated the effect of clinically relevant cumulative irradiation on TRAIL-induced apoptosis.

Methods And Materials: Using a previously established HN3 cell line from a laryngeal carcinoma patient, we generated a chronically irradiated HN3R isogenic cell line. Read More

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http://dx.doi.org/10.1016/j.ijrobp.2013.12.016DOI Listing
April 2014
19 Reads

Methods and matrices: approaches to identifying miRNAs for nasopharyngeal carcinoma.

J Transl Med 2014 Jan 6;12. Epub 2014 Jan 6.

Department of Microbiology, Immunology and Tropical Medicine, School of Medicine and Health Science, George Washington University, Washington, DC, USA.

Background: Nasopharyngeal carcinoma (NPC) is a solid tumor of the head and neck. Multimodal therapy is highly effective when NPC is detected early. However, due to the location of the tumor and the absence of clinical signs, early detection is difficult, making a biomarker for the early detection of NPC a priority. Read More

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http://dx.doi.org/10.1186/1479-5876-12-3DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3895762PMC
January 2014
12 Reads

[Expression of Six1 and Six4 in esophageal squamous cell carcinoma and their correlation with clinical prognosis].

Zhonghua Bing Li Xue Za Zhi 2013 Jul;42(7):446-50

Department of Pathology, Shanghai Medical College, Fudan University, Shanghai 200032, China.

Objective: Six1 and Six4 are expressed in several tumors, and associated with tumor progress and poor prognosis. The aim of this study was to investigate the expression of Six1 and Six4 in esophageal squamous cell carcinoma (ESCC), and to evaluate their correlation with the clinicopathological factors and prognosis.

Methods: Tissue microarray technology and immunohistochemical method (EnVision) were used to detect the expression of Six1 and Six4 in the tumor tissues and corresponding adjacent normal epithelium of esophagus from 292 ESCC patients. Read More

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http://dx.doi.org/10.3760/cma.j.issn.0529-5807.2013.07.004DOI Listing
July 2013
11 Reads

Radiation therapy and esophageal cancer.

Cancer Control 2013 Apr;20(2):97-110

Radiation Oncology Program, H Lee Moffitt Cancer Center & Research Institute, Tampa, FL 33612, USA.

Background: Squamous cell carcinoma and adenocarcinoma account for more than 90% of all esophageal cancer cases. Although the incidence of squamous cell carcinoma has declined, the incidence of adenocarcinoma has risen due to increases in obesity and gastroesophageal reflux disease.

Methods: The authors examine the role of radiation therapy alone (external beam and brachytherapy) for the management of esophageal cancer or combined with other modalities. Read More

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https://www.moffitt.org/File%20Library/Main%20Nav/Research%2
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http://dx.doi.org/10.1177/107327481302000203DOI Listing
April 2013
23 Reads

Wdr66 is a novel marker for risk stratification and involved in epithelial-mesenchymal transition of esophageal squamous cell carcinoma.

BMC Cancer 2013 Mar 21;13:137. Epub 2013 Mar 21.

Experimental Clinical Research Center at the Max-Delbrueck-Center for Molecular Medicine, Charité Campus Buch, Lindenbergerweg 80, Berlin, 13125, Germany.

Background: We attempted to identify novel biomarkers and therapeutic targets for esophageal squamous cell carcinoma by gene expression profiling of frozen esophageal squamous carcinoma specimens and examined the functional relevance of a newly discovered marker gene, WDR66.

Methods: Laser capture microdissection technique was applied to collect the cells from well-defined tumor areas in collaboration with an experienced pathologist. Whole human gene expression profiling of frozen esophageal squamous carcinoma specimens (n = 10) and normal esophageal squamous tissue (n = 18) was performed using microarray technology. Read More

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http://dx.doi.org/10.1186/1471-2407-13-137DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3610187PMC
March 2013
1 Read

Phosphorylated EGFR at tyrosine 1173 correlates with poor prognosis in oral squamous cell carcinomas.

Oral Dis 2014 Mar 6;20(2):178-85. Epub 2013 Mar 6.

Medicine and Oral Surgery Department, Dental Sciences Group - Health Sciences Research Centre, Higher Institute of Health Sciences (ISCSN), CESPU, Paredes, Portugal.

Objectives: To evaluate the expression of epidermal growth factor receptor (EGFR) and phosphorylated EGFR (pEGFR), in oral squamous cell carcinomas (OSCC). We examined their utility as prognostic markers by relating to clinicopathological characteristics and the clinical outcome.

Materials And Methods: We analysed 74 primary OSCC and examined immunohistochemical expression of EGFR and pEGFR (phosphorylated at tyrosine 1173) using tissue microarray technology. Read More

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http://doi.wiley.com/10.1111/odi.12087
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http://dx.doi.org/10.1111/odi.12087DOI Listing
March 2014
4 Reads

Comprehensive MicroRNA expression profiling identifies novel markers in follicular variant of papillary thyroid carcinoma.

Thyroid 2013 Nov 19;23(11):1383-9. Epub 2013 Sep 19.

1 Department of Pathology and Laboratory Medicine, University of Pittsburgh Medical Center , Pittsburgh, Pennsylvania.

Background: Follicular variant of papillary thyroid carcinoma (FVPTC) shares features of papillary (PTC) and follicular (FTC) thyroid carcinomas on a clinical, morphological, and genetic level. MicroRNA (miRNA) deregulation was extensively studied in PTCs and FTCs. However, very limited information is available for FVPTC. Read More

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http://dx.doi.org/10.1089/thy.2012.0632DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3822383PMC
November 2013
10 Reads
21 Citations
4.493 Impact Factor

Validation of a gene expression signature for assessment of lymph node metastasis in oral squamous cell carcinoma.

J Clin Oncol 2012 Nov 8;30(33):4104-10. Epub 2012 Oct 8.

Molecular Cancer Research, University Medical Center Utrecht, PO Box 85500, 3508 GA Utrecht, the Netherlands.

Purpose: Current assessment of lymph node metastasis in patients with head and neck squamous cell carcinoma is not accurate enough to prevent overtreatment. The aim of this study was validation of a gene expression signature for distinguishing metastasizing (N+) from nonmetastasizing (N0) squamous cell carcinoma of the oral cavity (OSCC) and oropharynx (OPSCC) in a large multicenter cohort, using a diagnostic DNA microarray in a Clinical Laboratory Improvement Amendments/International Organization for Standardization-approved laboratory.

Methods: A multigene signature, previously reported as predictive for the presence of lymph node metastases in OSCC and OPSCC, was first re-evaluated and trained on 94 samples using generic, whole-genome, DNA microarrays. Read More

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http://dx.doi.org/10.1200/JCO.2011.40.4509DOI Listing
November 2012
10 Reads

Prognostication and prediction using gene expression profiling in oesophageal cancer.

Eur J Surg Oncol 2013 Jan 25;39(1):17-23. Epub 2012 Jul 25.

Department of Surgery, The Netherlands Cancer Institute - Antoni van Leeuwenhoek Hospital, Plesmanlaan 121, 1066 CX Amsterdam, The Netherlands.

Aim: To evaluate current literature on gene expression profiling in oesophageal cancer.

Methods: We performed a review of the literature (2000-2010) on prognostication and prediction using gene expression analysis in oesophageal cancer.

Results: Seventeen papers comprising 638 patients were included. Read More

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http://dx.doi.org/10.1016/j.ejso.2012.07.008DOI Listing
January 2013
1 Read

Expression of CA-IX is associated with advanced stage tumors and poor survival in oral squamous cell carcinoma patients.

J Oral Pathol Med 2012 Oct 4;41(9):667-74. Epub 2012 Apr 4.

Department of Pathology, Forensic Sciences, University Hospital and School of Medicine of Santiago de Compostela, Servicio de Anatomia Patológica, Hospital Clinico de Santiago, Santiago de Compostela, Spain.

Introduction: Carbonic anhydrases (CAs), a group of ubiquitously expressed metalloenzymes, are involved in numerous physiological and pathological processes, including tumorigenicity. Specifically, CA-IX has been primarily found in hypoxic tumor tissues.

Material And Methods: This is a retrospective study of tumors from the Tissue Bank of the Pathology Department of the University Hospital of Santiago de Compostela. Read More

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http://dx.doi.org/10.1111/j.1600-0714.2012.01147.xDOI Listing
October 2012
8 Reads

Combined cytoplasmic and membranous EGFR and p53 overexpression is a poor prognostic marker in early stage oral squamous cell carcinoma.

J Oral Pathol Med 2012 Aug 14;41(7):559-67. Epub 2012 Mar 14.

Medicine and Oral Surgery Department, Dental Sciences Group-Health Sciences Research Centre, Instituto Superior de Ciências da Saúde Norte, Paredes, Portugal.

Objective: Our aim was to evaluate the expression of several molecules that regulate growth, the cell cycle and signalling pathways including EGFR, p53, p16 and p27 in oral squamous cell carcinomas (OSCC). We examined their utility as prognostic markers by relating to clinicopathological characteristics and the clinical outcome.

Patients And Methods: Using tissue microarray technology, we analysed 67 primary OSCC and examined immunohistochemical expression of EGFR, p53, p16 and p27. Read More

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http://dx.doi.org/10.1111/j.1600-0714.2012.01142.xDOI Listing
August 2012
5 Reads

Study of the differentially expressed genes in pleomorphic adenoma using cDNA microarrays.

Pathol Oncol Res 2011 Sep 9;17(3):765-9. Epub 2011 May 9.

Department of Stomatology, First People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.

Recent studies have determined that gene expression profiling using microarray technology can be used to identify tumor-related molecules. The objective of this study was to screen the differentially expressed genes between pleomorphic adenoma (PA) and the normal tissue adjacent to PA using cDNA microarrays and to further validate the differentially expressed genes by real-time PCR. In this study, we selected five pairs of PA and the surrounding normal salivary gland tissues. Read More

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http://link.springer.com/10.1007/s12253-011-9384-9
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http://dx.doi.org/10.1007/s12253-011-9384-9DOI Listing
September 2011
10 Reads

Metabolic imaging allows early prediction of response to vandetanib.

J Nucl Med 2011 Feb 13;52(2):231-40. Epub 2011 Jan 13.

Institute of Nuclear Medicine, University Hospital, Bern, Switzerland.

Unlabelled: The RET (rearranged-during-transfection protein) protooncogene triggers multiple intracellular signaling cascades regulating cell cycle progression and cellular metabolism. We therefore hypothesized that metabolic imaging could allow noninvasive detection of response to the RET inhibitor vandetanib in vivo.

Methods: The effects of vandetanib treatment on the full-genome expression and the metabolic profile were analyzed in the human medullary thyroid cancer cell line TT. Read More

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http://dx.doi.org/10.2967/jnumed.110.081745DOI Listing
February 2011
2 Reads

Prognostic biomarkers for esophageal adenocarcinoma identified by analysis of tumor transcriptome.

PLoS One 2010 Nov 30;5(11):e15074. Epub 2010 Nov 30.

Department of Systems Biology, University of Texas MD Anderson Cancer Center, Houston, Texas, United States of America.

Background: Despite many attempts to establish pre-treatment prognostic markers to understand the clinical biology of esophageal adenocarcinoma (EAC), validated clinical biomarkers or parameters remain elusive. We generated and analyzed tumor transcriptome to develop a practical biomarker prognostic signature in EAC.

Methodology/principal Findings: Untreated esophageal endoscopic biopsy specimens were obtained from 64 patients undergoing surgery and chemoradiation. Read More

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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0015074PLOS
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2994829PMC
November 2010
4 Reads

Is gene expression profiling of head and neck cancers ready for the clinic?

Biomark Med 2010 Aug;4(4):571-80

University of Pennsylvania Health System, Philadelphia, PA, USA.

One of the major challenges in the head and neck oncology clinic is the need to identify biomarkers and/or gene expression signatures that complement, strengthen and increase the sensitivity and specificity of the current clinicopathologic analyses. Microarray analysis of head and neck tumors has demonstrated that the combined influence of many genes or biomarkers can make superior identifiers and/or predictors of tumor behavior and patient outcome. Here, an update of the recent literature on the prognostic and predictive value of microarrays for patients with head and neck squamous cell carcinomas is presented. Read More

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http://dx.doi.org/10.2217/bmm.10.71DOI Listing
August 2010
4 Reads

Thyroid tumor marker genomics and proteomics: diagnostic and clinical implications.

J Cell Physiol 2010 Sep;224(3):612-9

Department of Reproduction and Ageing, University of Pisa, Pisa, Italy.

Two systems biology concepts, genomics and proteomics, are highlighted in this review. These techniques are implemented to optimize the use of thyroid tumor markers (TTM). Tissue microarray studies can produce genetic maps and proteomics, patterns of protein expression of TTM derived from preoperative biopsies and specimens. Read More

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http://doi.wiley.com/10.1002/jcp.22187
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http://dx.doi.org/10.1002/jcp.22187DOI Listing
September 2010
3 Reads

[Diagnostic and prognostic biomarkers in head and neck squamous cell carcinoma].

HNO 2010 Jul;58(7):713-23; quiz 724-5

Universitäts-Hals-Nasen-Ohren-Klinik, Universitätsklinikum Essen, Hufelandstrasse 55, 45122 Essen.

Classical prognostic factors for squamous cell carcinoma of the head and neck (HNSCC) are based on general parameters such as tumor stage or histological grading and only allow for a rough estimation of the clinical course. However, predicting individual responses to treatment remains challenging and diverging clinical courses of same-stage HNSCC stage remain obscure. The need for a better understanding of the individual genomic or proteomic signature of HNSCC resulted in a great number of publications on novel biomarkers. Read More

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http://dx.doi.org/10.1007/s00106-010-2108-8DOI Listing
July 2010
5 Reads

Neoadjuvant chemotherapy in Barrett's carcinoma - prognosis and response prediction.

Anticancer Res 2010 Apr;30(4):1065-70

Department of General, Visceral, and Children's Surgery, Universitaetsklinikum Duesseldorf, Heinrich Heine Universitaet, Duesseldorf, Germany.

In spite of endoscopic surveillance programs, 90% of patients initially presenting with Barrett's carcinoma have locally advanced disease. In these patients, preoperative chemotherapy increases the chance of a curative resection in responding patients. Unfortunately, response occurs in only 50% of patients after chemotherapy with cisplatin, 5-fluorouracil and leucovorin. Read More

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April 2010
7 Reads

Gene expression profile changes and possible molecular subtypes in differentiated-type nonkeratinizing nasopharyngeal carcinoma.

Int J Cancer 2011 Feb;128(4):753-62

Department of Pathology, School of Basic Medical Sciences, Southern Medical University, Guangzhou, China.

Nasopharyngeal carcinoma (NPC) is a human malignant tumor with a high incidence and a poor prognosis in Southern China and South-eastern Asia. In this study, we comprehensively analyzed the gene expression profiles in 24 samples of primary differentiated-type nonkeratining NPC (DNK-NPC) tissues, 24 samples of normal nasopharyngeal tissues and 4 DNK-NPC cell lines using cDNA microarray technology and bioinformatics methods. We found expression level of some genes was wildly alerted in the DNK-NPC samples. Read More

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http://dx.doi.org/10.1002/ijc.25392DOI Listing
February 2011
3 Reads

Molecular analysis of oral squamous cell carcinoma: a tissue microarray study.

Indian J Cancer 2010 Apr-Jun;47(2):166-72

Department of Oral Pathology, Manipal College of Dental Science, Manipal University, Manipal, Karnataka 576 104, India.

Background: An intriguing aspect of Oral Squamous Cell Carcinomas (OSCC) is its behavioral disparity. Among patients who present with the similar clinicopathological features, some have a better prognosis than others. Identification of molecular alterations responsible for this may contribute to a greater understanding of tumor behavior. Read More

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http://dx.doi.org/10.4103/0019-509X.63013DOI Listing
August 2010
2 Reads

EGFR and Ki-67 expression in oral squamous cell carcinoma using tissue microarray technology.

J Oral Pathol Med 2010 Aug 14;39(7):571-8. Epub 2010 Feb 14.

Department of Oral Surgery and Oral Medicine, Higher Institute of Health Sciences, Paredes, Portugal.

Objective: Our aim was to validate the use of tissue microarrays (TMA) in oral squamous cell carcinomas (OSCC) to analyse epidermal growth factor receptor (EGFR) and Ki-67 expression. We also analysed the relationship that the expression of these markers may have with clinical, pathological and survival variables.

Patients And Methods: The study sample comprised 39 unselected patients diagnosed and treated for OSCC. Read More

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http://dx.doi.org/10.1111/j.1600-0714.2009.00876.xDOI Listing
August 2010
15 Reads

Molecular profiling of laryngeal cancer.

Expert Rev Anticancer Ther 2009 Sep;9(9):1251-60

Department of Otorhinolaryngology-Head and Neck Surgery, Helsinki University Central Hospital, Helsinki, Finland.

Head and neck squamous cell carcinoma, including laryngeal cancer, is considered to progress from dysplasia to invasive cancer through well-defined clinical and histological stages. Numerous genetic alterations have been described in laryngeal squamous cell carcinoma, but the molecular mechanisms contributing to initiation and progression of laryngeal cancer are still poorly understood. Genomic, transcriptomic and protein alterations in laryngeal cancer progression are key areas for the current research aimed at finding instruments for classification, prognostication and patient-tailored treatment. Read More

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http://dx.doi.org/10.1586/era.09.102DOI Listing
September 2009
4 Reads

Identification of potential therapeutic targets in human head & neck squamous cell carcinoma.

Head Neck Oncol 2009 Jul 14;1:27. Epub 2009 Jul 14.

Tumor Vaccines and Biotechnology Branch, Division of Cellular and Gene Therapies, Center for Biologics Evaluation and Research, Food and Drug Administration, Bethesda, MD 20892, USA.

Background: Human head and neck squamous cell carcinoma (HNSCC) is an aggressive and recurrent malignancy. Identification of unique or overexpressed cell-associated or cell surface antigens is critical for diagnosis and development of cancer vaccines and targeted therapies for HNSCC. We have used high throughput microarray technology to search for candidate targets in HNSCC. Read More

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http://dx.doi.org/10.1186/1758-3284-1-27DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2719634PMC
July 2009
3 Reads

Three-dimensional endoscopic pituitary surgery.

Neurosurgery 2009 May;64(5 Suppl 2):288-93; discussion 294-5

Department of Otolaryngology-Head and Neck Surgery, New York Presbyterian Hospital, Weill Medical College of Cornell University, New York, New York 10065, USA.

Objective: We describe a novel 3-dimensional (3-D) stereoendoscope and discuss our early experience using it to provide improved depth perception during transsphenoidal pituitary surgery.

Methods: Thirteen patients underwent endonasal endoscopic transsphenoidal surgery. A 6. Read More

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http://dx.doi.org/10.1227/01.NEU.0000338069.51023.3CDOI Listing
May 2009
3 Reads

Building a bioresource for esophageal research: lessons from the early experience of an academic medical center.

Dis Esophagus 2010 Jan 15;23(1):1-7. Epub 2009 Apr 15.

Department of Surgery, Trinity Centre for Health Sciences, St James's Hospital, Trinity College Dublin, Ireland.

The establishment of biorepositories, linked to clinical and epidemiologic data, are central to the goals of personalized medicine and individualized cancer therapy. Repositories of DNA, RNA, and serum samples are valuable resources for cancer research, enabling the investigation of the underlying causes of cancer development, progression, and prognosis, as well as providing a resource for the investigation of biomarkers for early detection and prediction of response. With a greater reliance on sample-derived data for molecular-based research and clinical care, improved standards and informatics for sample procurement, storage, and analysis are necessary to maximize the value of tissue collection for research participants, investigators, and academic medical centers. Read More

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http://dx.doi.org/10.1111/j.1442-2050.2009.00969.xDOI Listing
January 2010
2 Reads

Expression profile of metastasis-related genes in invasive oral cancers.

Histol Histopathol 2008 10;23(10):1213-22

Department of Dental Laboratory Technology, Central Taiwan University of Science and Technology, Taichung, Taiwan.

We used suppression subtractive hybridization (SSH) and oligonucleotide microarray to differentiate expression profiles of metastasis-related genes and to evaluate their clinical significance in patients with invasive oral cancer (OCa). Overexpression of the specific genes was confirmed by reverse transcription-PCR (RT-PCR). Cells expressing the gene were identified by immunohistochemistry in pathology specimens. Read More

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http://dx.doi.org/10.14670/HH-23.1213DOI Listing
October 2008
1 Read

An ultrasensitive new DNA microarray chip provides gene expression profiles for preoperative esophageal cancer biopsies without RNA amplification.

Oncology 2007 30;73(5-6):366-75. Epub 2008 May 30.

Division of Gastroenterology, Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, MD, USA.

Objectives: Gene expression profiling using pretreatment biopsies has been limited due to their small sample sizes. This study evaluated the usefulness of an ultrasensitive new DNA microarray chip, which has a unique array structure, for the clinical diagnosis of esophageal cancer using preoperative biopsies.

Methods: Paired cancer and normal esophageal epithelial tissues from 56 patients who underwent esophagectomy and from 48 patients who underwent preoperative endoscopy were studied. Read More

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http://dx.doi.org/10.1159/000136156DOI Listing
August 2008
12 Reads

Exploration of metastasis-related proteins as biomarkers and therapeutic targets in the treatment of head and neck cancer.

Curr Cancer Drug Targets 2007 Nov;7(7):613-22

Department of Hematology and Oncology, Emory University Winship Cancer Institute, 1365 Clifton Road, Building C, Suite C3086, Atlanta, GA 30322, USA.

Head and neck (HN) cancer is a significant health-care problem worldwide. One of the major prognostic factors for squamous cell carcinomas of the head and neck (HNSCC) is metastasis. Early detection of lymph node metastasis and the identification of key protein targets for the treatment of metastatic HNSCC remain a challenge in current cancer research. Read More

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November 2007
3 Reads

Nasopharyngeal cancer: new frontiers from the laboratory to the clinic.

Authors:
Fei-Fei Liu

Int J Radiat Oncol Biol Phys 2007 ;69(2 Suppl):S122-4

Division of Applied Molecular Oncology, Ontario Cancer Institute, University Health Network, Toronto, Ontario, Canada.

Nasopharyngeal carcinoma (NPC) is a unique malignant epithelial carcinoma of the head-and-neck region, with unique viral biology and geographic distributions in the world. Significant advances have been made in the clinical therapies for NPC, with novel techniques for radiation delivery and randomized trials demonstrating the survival advantage of concurrent chemotherapy for patients with locally advanced diseases. At the same time, there has been an improved understanding of the molecular dysregulations underpinning this disease, with recent discoveries emerging from the use of high-throughput technologies, including microarray expression profiling and robotic screens of chemical libraries. Read More

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http://dx.doi.org/10.1016/j.ijrobp.2007.05.070DOI Listing
November 2007
1 Read

Pathogenesis, diagnosis and therapeutic possibilities of esophageal cancer.

Authors:
Peter D Siersema

Curr Opin Gastroenterol 2007 Jul;23(4):456-61

Department of Gastroenterology and Hepatology, University Medical Center, Utrecht, The Netherlands.

Purpose Of Review: This article reviews developments in pathogenesis, diagnosis and therapy of esophageal cancer published in 2006.

Recent Findings: Gene expression profiles in esophageal adenocarcinoma reveal information on its pathogenesis. One study disputed the UK guideline to perform upper endoscopy only in the presence of alarm symptoms. Read More

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http://dx.doi.org/10.1097/MOG.0b013e32816a91deDOI Listing
July 2007
21 Reads

Identification of a predictive gene expression signature of cervical lymph node metastasis in oral squamous cell carcinoma.

Cancer Sci 2007 May 28;98(5):740-6. Epub 2007 Mar 28.

Department of Molecular Genetics, Medical Research Institute, Tokyo Medical and Dental University, Tokyo, Japan.

An accurate assessment of the cervical lymph node metastasis status in oral cavity cancer not only helps predict the prognosis of patients, but also helps surgeons to perform the appropriate treatment. We investigated the utilization of microarray technology focusing on the differences in gene expression profiles between primary tumors of oral squamous cell carcinoma that had metastasized to cervical lymph nodes and those that had not metastasized in the hope of finding new biomarkers to serve for diagnosis and treatment of oral cavity cancer. To design this experiment, we prepared two groups: the learning case group with 30 patients and the test case group with 13 patients. Read More

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http://dx.doi.org/10.1111/j.1349-7006.2007.00454.xDOI Listing
May 2007
8 Reads

Identification of a gene signature for rapid screening of oral squamous cell carcinoma.

Clin Cancer Res 2006 Oct;12(20 Pt 1):5960-71

Department of Otorhinolaryngology-Head and Neck Surgery, Center for Clinical Epidemiology and Biostatistics, University of Pennsylvania Health System, Philadelphia, Pennsylvania 19104, USA.

Purpose: Oral cancer is a major health problem worldwide and in the U.S. The 5-year survival rate for oral cancer has not improved significantly over the past 20 years and remains at approximately 50%. Read More

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http://clincancerres.aacrjournals.org/content/12/20/5960.ful
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http://clincancerres.aacrjournals.org/cgi/doi/10.1158/1078-0
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http://dx.doi.org/10.1158/1078-0432.CCR-06-0535DOI Listing
October 2006
5 Reads

[Gene expression profiling for prediction of response to chemotherapy].

Gan To Kagaku Ryoho 2006 Jan;33(1):1-5

Cancer Institute Japanese Foundation For Cancer Research, Genome Center.

Numerous genes whose expression is controlled by complex regulatory networks are involved in the development and progression of each cancer,and those genes will be the key factors for determining each characteristic of the tumor. The recent development of DNA microarray and related technologies provides an opportunity to perform more detailed characterization( profiling) of individual tumor cells. Indeed, the gene expression profile of a tumor provides a unique molecular portrait or signature that can be correlated with clinical behavior and drug responsiveness. Read More

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January 2006
3 Reads

[Expression of S-100 positive dendritic cell, TIMP-1 and p63 and its significance in the primary laryngeal carcinoma].

Lin Chuang Er Bi Yan Hou Ke Za Zhi 2005 Feb;19(3):127-9

Department of Otolaryngology-Head and Neck Surgery, First People's Hospital of Shanghai Jiaotong University, Shanghai 200080, China.

Objective: To evaluate the predictive role of S-100 positive dendritic cell, tissue inhibitor of metalloproteinases-1 (TIMP-1) and p63 gene in primary laryngeal carcinoma with epidemiology (smoking and drinking), histological grading, surgical treatment, TNM stage and prognosis by the tissuechip technology.

Methods: We studied the expression of dendritic cell (S-100), TIMP-1 and p63 gene on a series of 85 primary laryngeal carcinoma patients who had ever received in our hospital between 1992 and 2000 by the tissuechip technology and SP method. The correlation of each score according to the intensity and percentage of labeled cells or intercellular substance with relevant clinical dada was statistically analyzed. Read More

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February 2005
6 Reads