Search Our Scientific Publications & Authors

BMC Cancer 2020 Mar 14;20(1):218. Epub 2020 Mar 14.

Department of Oral Biology, Faculty of Dentistry, Mahidol University, Payathai Rd., Ratchathewi, Bangkok, 10400, Thailand.

Background: Oral squamous cell carcinoma (OSCC) is an aggressive human malignancy. Because of late diagnosis and recurrence of OSCC, the treatment of patients with OSCC is often ineffective. Thus, finding novel biomarkers of OSCC are essential. Read More

Virchows Arch 2020 May 11;476(5):725-734. Epub 2019 Dec 11.

Institute of Pathology, University of Bern, Murtenstrasse 31, 3008, Bern, Switzerland.

Esophageal carcinoma (EC) is one of the most aggressive human malignancies with high rates of resistance to conventional anticancer treatment. Cancer-associated fibroblasts (CAFs) are an important part of the tumor microenvironment and associated with tumor progression. COL11A1, SPARC, and CD90 have been identified as rather specific CAF markers, with COL11A1 expression particularly shown to influence response to chemotherapy. Read More

EBioMedicine 2019 Aug 16;46:54-65. Epub 2019 Jul 16.

Department of Thoracic Surgery, The First Affiliated Hospital of Nanjing Medical University, Nanjing, China. Electronic address:

Background: Esophageal squamous cell carcinoma (ESCC) is one of the most lethal malignancies with poor prognosis. Cancer-testis genes (CTGs) have been vigorously pursued as targets for cancer immunotherapy, but the expressive patterns and functional roles of CTGs remain unclear in ESCC.

Methods: A systematic screening strategy was adopted to screen CTGs in ESCC by integrating multiple public databases and RNA expression microarray data from 119 ESCC subjects. Read More

PLoS One 2019 16;14(7):e0218989. Epub 2019 Jul 16.

Integrated Head and Neck Oncology Program, Mazumdar Shaw Centre for Translational Research, Mazumdar Shaw Medical Foundation, Narayana Health, Bangalore, Karnataka, India.

Head and neck squamous cell carcinomas (HNSCC) includes multiple subsites that exhibit differential treatment outcome, which is in turn reflective of tumor stage/histopathology and molecular profile. This study hypothesized that the molecular profile is an accurate prognostic adjunct in patients triaged based on clinico-pathological characteristics. Towards this effect, publically available micro-array datasets (n = 8), were downloaded, classified based on HPV association (n = 83) and site (tongue n = 88; laryngopharynx n = 53; oropharynx n = 51) and re-analyzed (Genespring; v13. Read More

Biomolecules 2019 06 27;9(7). Epub 2019 Jun 27.

Cancer Biology Laboratory & DBT-AIST International Laboratory for Advanced Biomedicine (DAILAB), Department of Biosciences and Bioengineering, Indian Institute of Technology Guwahati, Guwahati, Assam 781039, India.

Protein kinase B (Akt) plays a very significant role in various cancers including oral cancer. However, it has three isoforms (Akt1, Akt2, and Akt3) and they perform distinct functions and even play contrasting roles in different cancers. Therefore, it becomes essential to evaluate the isoform-specific role of Akt in oral cancer. Read More

Mol Carcinog 2019 10 10;58(10):1809-1821. Epub 2019 Jun 10.

State Key Laboratory of Oral Diseases, Department of Oral and Maxillofacial Surgery, National Clinical Research Center for Oral Diseases, West China Hospital of Stomatology Sichuan University, Chengdu, Sichuan, China.

Macrophage migration inhibitory factor (MIF) has been shown to closely associate with the malignant progression of a variety of human carcinomas. However, the role and its underlying molecular mechanisms of MIF in the invasion and metastasis of oral squamous cell carcinoma (OSCC) still remains unclear. Here, we found that MIF silencing reduced the cell proliferation, migration, and invasion, as well as matrix metalloprotein-2 (MMP-2) and MMP-9 in OSCC cells. Read More

Interact Cardiovasc Thorac Surg 2019 10;29(4):510-516

Department of Thoracic Surgery, First Affiliated Hospital of Nanjing Medical University, Nanjing, China.

Objectives: Melanoma-associated antigen A1 (MAGEA1) is a potential target for immunotherapy and has been associated with poor survival rate in several cancers. However, little is known about the prognostic predictive value of MAGEA1 in oesophageal squamous cell carcinoma (OSCC). This study aims to determine whether the expression of MAGEA1 is an independent predictor of survival in patients with resectable OSCC. Read More

Neoplasia 2019 06 29;21(6):571-581. Epub 2019 Apr 29.

Department of Preventive Medicine, Institute of Biomedical Informatics, Joint National Laboratory for Antibody Drug Engineering, Cell Signal Transduction Laboratory, School of Basic Medical Sciences, Henan University, Kaifeng 475004, China. Electronic address:

Esophageal squamous cell carcinoma (ESCC) is one of the most common cancers worldwide, particularly in Asian populations, and responds poorly to conventional therapy. Subclassification of ESCCs by molecular analysis is a powerful strategy in extending conventional clinicopathologic classification, improving prognosis and therapy. Here we identified two ESCC molecular subtypes in Chinese population using gene expression profiling data and further validated the molecular subtypes in two other independent Asian populations (Japanese and Vietnamese). Read More

Theranostics 2019 28;9(6):1599-1613. Epub 2019 Feb 28.

The University of Hong Kong-Shenzhen Institute of Research and Innovation (HKU-SIRI), China.

Dysregulated microRNA (miRNA) expressions in cancer can contribute to chemoresistance. This study aims to identify miRNAs that are associated with fluorouracil (5-FU) chemoresistance in esophageal squamous cell carcinoma (ESCC). The potential of miR-29c as a novel diagnostic, prognostic and treatment-predictive marker in ESCC, and its mechanisms and therapeutic implication in overcoming 5-FU chemoresistance were explored. Read More

World J Surg Oncol 2019 Apr 1;17(1):60. Epub 2019 Apr 1.

Department of Radiation Oncology, Zhejiang Cancer Hospital, Hangzhou, Zhejiang, China.

Background: The incidence of nasopharyngeal carcinoma (NPC) is rare, but a certain amount of mortality remains in NPC patients. Our study aimed to identify candidate genes as biomarkers for NPC screening, diagnosis, and therapy.

Methods: We investigated two microarray profile datasets GSE64634 and GSE12452 to screen the potential differentially expressed genes (DEGs) in NPC. Read More

Arch Oral Biol 2019 Mar 4;99:92-106. Epub 2019 Jan 4.

Integrated Head and Neck Oncology Program, Mazumdar Shaw Medical Foundation, Mazumdar Shaw Medical Center, Narayana Health City, Bommasandra Industrial Area, Anekal Taluk, Bangalore, 560099, Karnataka, India; Department of Head and Neck Oncology, Mazumdar Shaw Medical Center, Narayana Health City, Bommasandra Industrial Area, Anekal Taluk, Bangalore, 560099, Karnataka, India; Mazumdar Shaw Medical Centre-Roswell Park Collaboration Program, Roswell Park Cancer Institute, Elm and Carlton Streets, Buffalo, 14263, New York, USA. Electronic address:

Objective: This study aimed to identify the cancer stem cell specific biomarkers that can be effective candidate prognosticators of oral squamous cell carcinoma.

Design: Microarray-based meta-analysis derived transcriptional profile of head and neck cancers was compared with the Cancer Stem Cell database to arrive at a subset of markers. This subset was further co-related with clinico-pathological parameters, recurrence and survival of oral cancer patients (n = 313) in The Cancer Genome Atlas database and in oral cancer (n = 28) patients. Read More

Cancer Biomark 2019 ;24(1):71-83

Department of Microbiology and Immunology, New York Medical College, Valhalla, NY, USA.

Background: Thyroid cancer is the most common endocrine malignancy worldwide, with the predominant form papillary thyroid carcinoma (PTC) representing approximately 80% of cases.

Objective: This study was addressed to identify potential genes and pathways involved in the pathogenesis of PTC and potential novel biomarkers for this disease.

Methods: Gene expression profiling was carried out by DNA microarray technology. Read More

J Transl Med 2019 01 3;17(1). Epub 2019 Jan 3.

Department of Gastroenterological Surgery, Nagoya University Graduate School of Medicine, 65, Tsurumai-cho, Showa-ku, Nagoya, 466-8550, Japan.

Background: Neoadjuvant chemotherapy (NAC) has become the standard of care for resectable esophageal squamous cell carcinoma (ESCC) which is one of the most lethal cancers, to improve resectability and prognosis. On this basis, to provide individually optimized therapy for ESCC, a minimally-invasive biomarker for response to NAC is strongly desired. This study aimed to identify the miRNA signature in serum specimens taken from ESCC patients undergoing NAC through genome-wide microarray technology. Read More

Acta Neuropathol Commun 2018 10 19;6(1):107. Epub 2018 Oct 19.

Department of Pathology and Laboratory Medicine, Medical University of South Carolina, 171 Ashley Ave, Charleston, 29425, SC, USA.

Endolymphatic sac tumor (ELST) is a rare neoplasm arising in the temporal petrous region thought to originate from endolymphatic sac epithelium. It may arise sporadically or in association with Von-Hippel-Lindau syndrome (VHL). The ELST prevalence in VHL ranges from 3 to 16% and may be the initial presentation of the disease. Read More

Cancer Gene Ther 2019 03 4;26(3-4):103-113. Epub 2018 Sep 4.

Department of Nuclear Medicine, Seventh People's Hospital of Shanghai University of Traditional Chinese Medicine, Shanghai, China.

To explore the mechanisms of GINS2 on cell proliferation and apoptosis in thyroid cancer (TC) cells. Expressions of GINS2 were inhibited in K1 and SW579 cells using gene interference technology. The abilities of proliferation and apoptosis, and cell cycle were determined by MTT assay and flow cytometric assay. Read More

Hum Pathol 2018 11 25;81:245-254. Epub 2018 Jul 25.

University Medical Center Utrecht, Department of Surgery, 3584CX Utrecht, The Netherlands. Electronic address:

Cancer Med 2018 08 28;7(8):3848-3861. Epub 2018 Jun 28.

Shenzhen Key Laboratory of Viral Oncology, the Clinical Innovation & Research Center (CIRC), Shenzhen Hospital, Southern Medical University, Shenzhen, China.

The major histocompatibility complex (MHC) is most closely associated with nasopharyngeal carcinoma (NPC), but the complexity of its genome structure has proven challenging for the discovery of causal MHC loci or genes. We conducted a targeted MHC sequencing in 40 Cantonese NPC patients followed by a two-stage replication in 1065 NPC cases and 2137 controls of Southern Chinese descendent. Quantitative RT-PCR analysis (qRT-PCR) was used to detect gene expression status in 108 NPC and 43 noncancerous nasopharyngeal (NP) samples. Read More

J BUON 2018 Mar-Apr;23(2):433-438

ENT Department, Medical School, University of Thessaly, Larissa, Greece.

Purpose: Human papillomavirus (HPV) is implicated in carcinogenesis of a variety of epithelia, including oropharyngeal and laryngeal. High risk (HR) HPV persistent infection in head and neck squamous cell carcinomas (HNSCC) is a significant event, but its influence regarding the prognosis and survival in these patients remains under consideration. Our aim was to analyze a series of oropharyngeal (OP) SCCs at the HPV DNA level, correlating them to the survival status of the corresponding patients. Read More

Cell Commun Signal 2018 04 27;16(1):19. Epub 2018 Apr 27.

Department of Cell and Molecular Biology, College of Medicine, Chang Gung University, Taoyuan, Taiwan.

Background: Golgin-97 is a tethering factor in the trans-Golgi network (TGN) and is crucial for vesicular trafficking and maintaining cell polarity. However, the significance of golgin-97 in human diseases such as cancer remains unclear.

Methods: We searched for a potential role of golgin-97 in cancers using Kaplan-Meier Plotter ( http://kmplot. Read More

Methods Mol Biol 2018 ;1756:105-118

Cancer Molecular Pathology of School of Medicine, Griffith University, Gold Coast, Australia.

Tissue microarray technology could allow immunohistochemical staining or in situ hybridization on hundreds of different tissue samples simultaneously. It allows faster analysis and considerably reducing costs incurred in staining. The technique also provides a high-throughput analysis of multiple tissues for the different types of research. Read More

Lancet Oncol 2018 03 7;19(3):382-393. Epub 2018 Feb 7.

Sun Yat-sen University Cancer Center, State Key Laboratory of Oncology in South China, Collaborative Innovation Center of Cancer Medicine, Guangzhou, China. Electronic address:

Background: Gene expression patterns can be used as prognostic biomarkers in various types of cancers. We aimed to identify a gene expression pattern for individual distant metastatic risk assessment in patients with locoregionally advanced nasopharyngeal carcinoma.

Methods: In this multicentre, retrospective, cohort analysis, we included 937 patients with locoregionally advanced nasopharyngeal carcinoma from three Chinese hospitals: the Sun Yat-sen University Cancer Center (Guangzhou, China), the Affiliated Hospital of Guilin Medical University (Guilin, China), and the First People's Hospital of Foshan (Foshan, China). Read More

Bioinformatics 2018 05;34(10):1767-1773

Department of Surgery, Mackay Memorial Hospital, Taipei, Taiwan.

Motivation: The aim of precision medicine is to harness new knowledge and technology to optimize the timing and targeting of interventions for maximal therapeutic benefit. This study explores the possibility of building AI models without precise pixel-level annotation in prediction of the tumor size, extrathyroidal extension, lymph node metastasis, cancer stage and BRAF mutation in thyroid cancer diagnosis, providing the patients' background information, histopathological and immunohistochemical tissue images.

Results: A novel framework for objective evaluation of automatic patient diagnosis algorithms has been established under the auspices of the IEEE International Symposium on Biomedical Imaging 2017- A Grand Challenge for Tissue Microarray Analysis in Thyroid Cancer Diagnosis. Read More

Eur Arch Otorhinolaryngol 2018 Feb 9;275(2):535-543. Epub 2017 Dec 9.

Department of Oral Pathology, College of Dentistry, Gangneung-Wonju National University, Gangneung, South Korea.

Purpose: Human papilloma virus (HPV) is the main source of cervical cancer. Many recent studies have revealed the prevalence and prognosis of HPV associated with oropharyngeal squamous cell carcinoma, but fewer reports have evaluated HPV in oral squamous cell carcinoma (OSCC). The purpose of this study was to determine the prevalence and prognosis of HPV associated with OSCC according to HPV and tumor types. Read More

Br J Cancer 2017 Dec 7;117(12):1837-1845. Epub 2017 Nov 7.

Department of Surgery (A), Heinrich-Heine-University and University Hospital Duesseldorf, Moorenstr. 5, Duesseldorf 40225, Germany.

Background: Medullary thyroid carcinoma (MTC) is a rare and challenging endocrine malignancy. Once spread, the therapeutic options are limited and the outcome poor. For these patients, the identification of new druggable biological markers is of great importance. Read More

Sci Rep 2017 09 7;7(1):10932. Epub 2017 Sep 7.

Department of Thoracic Surgery, Korea Cancer Center Hospital, Korea Institute of Radiological and Medical Sciences, Seoul, Korea.

Radioresistance is a challenge in the treatment of esophageal squamous cell carcinoma (ESCC). MicroRNAs (miRNAs) are known to play an important role in the functional modification of cancer cells and recent studies have reported miRNA-mediated radiotherapy resistance. However, further research is necessary to reveal the regulation mechanisms, and treatment strategies using miRNA are yet to be established for ESCC. Read More

Cancer Immunol Immunother 2017 Dec 30;66(12):1619-1629. Epub 2017 Aug 30.

Pathology, University of Helsinki, HUSLAB, and Helsinki University Hospital, P.O Box 21, 00014 University of Helsinki, Helsinki, Finland.

A large subset of oropharyngeal squamous cell carcinomas (OPSCCs) is associated with HPV infection and has better outcome than non-viral-related tumors. Various malignancies also carry a role for TLRs, key activators of inflammation and innate immunity. We examined the expression of TLRs in OPSCC, and their association with HPV status and treatment outcome. Read More

J Huazhong Univ Sci Technolog Med Sci 2017 Jun 6;37(3):319-325. Epub 2017 Jun 6.

Cancer Biology Research Center & Key Laboratory of the Ministry of Education, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China.

Esophageal cancer is a common malignant tumor, whose pathogenesis and prognosis factors are not fully understood. This study aimed to discover the gene clusters that have similar functions and can be used to predict the prognosis of esophageal cancer. The matched microarray and RNA sequencing data of 185 patients with esophageal cancer were downloaded from The Cancer Genome Atlas (TCGA), and gene co-expression networks were built without distinguishing between squamous carcinoma and adenocarcinoma. Read More

Oncotarget 2017 Jun;8(24):38755-38766

School of Biomedical Sciences, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Pokfulam, Hong Kong SAR, China.

Metastasis is the most lethal hallmark of esophageal squamous cell carcinoma (ESCC). The aim of the study is to identify key signaling pathways that control metastasis in ESCC. Highly invasive ESCC sublines (designated I3 cells) were established through three rounds of selection of cancer cells invading through matrigel-coated chambers. Read More

Clin Cancer Res 2016 Oct 28;22(19):4880-4889. Epub 2016 Jun 28.

Hamon Center for Therapeutic Oncology Research, University of Texas Southwestern Medical Center, Dallas, Texas. Simmons Cancer Center, University of Texas Southwestern Medical Center, Dallas, Texas. Department of Pathology, University of Texas Southwestern Medical Center, Dallas, Texas.

Purpose: Most non-small cell lung cancers (NSCLC) are now diagnosed from small specimens, and classification using standard pathology methods can be difficult. This is of clinical relevance as many therapy regimens and clinical trials are histology dependent. The purpose of this study was to develop an mRNA expression signature as an adjunct test for routine histopathologic classification of NSCLCs. Read More

Oncotarget 2016 May;7(18):26604-16

State Key Laboratory of Oncology in South China, Collaborative Innovation Center for Cancer Medicine, Sun Yat-sen University Cancer Center, Guangzhou, Guangdong, China.

Nasopharyngeal carcinoma (NPC) is well known as one of the most common malignancies in southern China and Southeast Asia. However, the mechanisms underlying NPC progression remain poorly understood. Herein, through overlapping the differentially expressed genes from 3 microarray data sets with the human kinome, we identified PBK, a serine-threonine kinase, is highly upregulated and has not been intensively investigated in NPC. Read More

Eur J Nucl Med Mol Imaging 2016 Jul 26;43(7):1267-77. Epub 2016 Jan 26.

Department of Nuclear Medicine and Endocrine Oncology, Maria Skłodowska-Curie Memorial Cancer Center and Institute of Oncology, Gliwice Branch, Wybrzeze Armii Krajowej 15, 44-101, Gliwice, Poland.

Purpose: Following the nuclear accidents in Chernobyl and later in Fukushima, the nuclear community has been faced with important issues concerning how to search for and diagnose biological consequences of low-dose internal radiation contamination. Although after the Chernobyl accident an increase in childhood papillary thyroid cancer (PTC) was observed, it is still not clear whether the molecular biology of PTCs associated with low-dose radiation exposure differs from that of sporadic PTC.

Methods: We investigated tissue samples from 65 children/young adults with PTC using DNA microarray (Affymetrix, Human Genome U133 2. Read More

PLoS One 2016 25;11(1):e0147409. Epub 2016 Jan 25.

Integrated Head and Neck Oncology Program, Mazumdar Shaw Centre for Translational Research, Mazumdar Shaw Medical Centre, Narayana Health, Bangalore, Karnataka, India.

The head and neck squamous cell carcinoma (HNSCC) transcriptome has been profiled extensively, nevertheless, identifying biomarkers that are clinically relevant and thereby with translational benefit, has been a major challenge. The objective of this study was to use a meta-analysis based approach to catalog candidate biomarkers with high potential for clinical application in HNSCC. Data from publically available microarray series (N = 20) profiled using Agilent (4X44K G4112F) and Affymetrix (HGU133A, U133A_2, U133Plus 2) platforms was downloaded and analyzed in a platform/chip-specific manner (GeneSpring software v12. Read More

J Oral Pathol Med 2016 May 5;45(5):329-37. Epub 2015 Oct 5.

Centre for Biotechnology, University of Turku and Åbo Akademi University, Turku, Finland.

Background: CIP2A, an inhibitor of PP2A tumour suppressor function, is a widely overexpressed biomarker of aggressive disease and poor therapy response in multiple human cancer types.

Methods: CIP2A and DPPA4 copy number alterations and expression were analysed by fluorescence in situ hybridisation (FISH) and immunohistochemistry (IHC) in different cell lines and a tissue microarray of 52 HNSCC patients. Results were correlated with patient survival and other clinicopathological data. Read More

ACM BCB 2015 Sep;2015:393-402

Department of Biomedical Engineering Georgia Institute of Technology Atlanta, GA 1-404-385-5059.

Increased understanding of the transcriptomic patterns underlying head and neck squamous cell carcinoma (HNSCC) can facilitate earlier diagnosis and better treatment outcomes. Integrating knowledge from multiple studies is necessary to identify fundamental, consistent gene expression signatures that distinguish HNSCC patient samples from disease-free samples, and particularly for detecting HNSCC at an early pathological stage. This study utilizes feature integration and heterogeneous ensemble modeling techniques to develop robust models for predicting HNSCC disease status in both microarray and RNAseq datasets. Read More

Tumour Biol 2016 Feb 27;37(2):1671-81. Epub 2015 Aug 27.

Cancer Research Center, The Key Laboratory of Brain and Spinal Cord Injury of Liaoning Province, Laboratory Animal Center, The First Affiliated Hospital of Liaoning Medical University, Jinzhou, 121001, China.

Rho signaling component, α-catulin, is a cytoskeletal linker protein and plays an important role in apoptotic and senescence resistance, cytoskeletal reorganization, mobility, invasion, and epithelial to mesenchymal transition (EMT) of cancer cells. Here, we transfected α-catulin-expressing plasmid into head and neck squamous cell carcinoma (HNSCC) cell and examined the phenotypes and relevant molecules. α-catulin expression was detected on tissue microarray containing squamous epithelium, dysplasia, and cancer of head and neck by immunohistochemistry. Read More

Genes Chromosomes Cancer 2015 Apr 29;54(4):249-59. Epub 2014 Dec 29.

Serviço de Anatomia Patológica, Instituto Português de Oncologia Francisco Gentil, Lisboa, Portugal & Instituto de Anatomia Patológica, Faculdade de Medicina de Lisboa, Portugal.

Diverse microarray and sequencing technologies have been widely used to characterize molecular changes in malignant epithelial cells in salivary neoplasms. Such gene expression studies to identify markers and targets in tumor cells are, however, compromised by the cellular heterogeneity of these tumors and by the difficulties to accrue matching controls representing normal salivary glands. Seventeen samples of primary salivary epithelial-myoepithelial carcinoma along with tissue from six normal major salivary glands were microdissected from paraffin-embedded tissue. Read More

Lin Chung Er Bi Yan Hou Tou Jing Wai Ke Za Zhi 2014 Jul;28(13):975-8

Objective: To filtrate and prove the different microRNAs (miRs) profiles in nasopharyngeal carcinoma.

Method: Screening the different expressions of miRs between nasopharyngeal carcinoma and the inflammatory tissues by the application of expression profiling of chip high-throughput and large-scale microarray analysis. Then we used RT-QPCR technology to prove the accuracy of screening results. Read More

Head Neck Pathol 2015 Jun 19;9(2):223-35. Epub 2014 Sep 19.

Department of Otolaryngology and Head and Neck Oncology Program, Winship Cancer Institute of Emory University, Emory University School of Medicine, Atlanta, GA, USA,

The role of molecular methods in the diagnosis of head and neck cancer is rapidly evolving and holds great potential for improving outcomes for all patients who suffer from this diverse group of malignancies . However, there is considerable debate as to the best clinical approaches, particularly for Next Generation Sequencing (NGS). The choices of NGS methods such as whole exome, whole genome, whole transcriptomes (RNA-Seq) or multiple gene resequencing panels, each have strengths and weakness based on data quality, the size of the data, the turnaround time for data analysis, and clinical actionability. Read More

Int J Radiat Oncol Biol Phys 2014 Apr;88(5):1203-11

Department of Otolaryngology, Asan Medical Center, University of Ulsan College of Medicine, Seoul, Republic of Korea; Biomedical Research Institute, Korea Institute of Science and Technology, Seoul, Republic of Korea. Electronic address:

Purpose: There are limited therapeutic options for patients with recurrent head and neck cancer after radiation therapy failure. To assess the use of tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) as a salvage chemotherapeutic agent for recurrent cancer after radiation failure, we investigated the effect of clinically relevant cumulative irradiation on TRAIL-induced apoptosis.

Methods And Materials: Using a previously established HN3 cell line from a laryngeal carcinoma patient, we generated a chronically irradiated HN3R isogenic cell line. Read More

J Transl Med 2014 Jan 6;12. Epub 2014 Jan 6.

Department of Microbiology, Immunology and Tropical Medicine, School of Medicine and Health Science, George Washington University, Washington, DC, USA.

Background: Nasopharyngeal carcinoma (NPC) is a solid tumor of the head and neck. Multimodal therapy is highly effective when NPC is detected early. However, due to the location of the tumor and the absence of clinical signs, early detection is difficult, making a biomarker for the early detection of NPC a priority. Read More

Zhonghua Bing Li Xue Za Zhi 2013 Jul;42(7):446-50

Department of Pathology, Shanghai Medical College, Fudan University, Shanghai 200032, China.

Objective: Six1 and Six4 are expressed in several tumors, and associated with tumor progress and poor prognosis. The aim of this study was to investigate the expression of Six1 and Six4 in esophageal squamous cell carcinoma (ESCC), and to evaluate their correlation with the clinicopathological factors and prognosis.

Methods: Tissue microarray technology and immunohistochemical method (EnVision) were used to detect the expression of Six1 and Six4 in the tumor tissues and corresponding adjacent normal epithelium of esophagus from 292 ESCC patients. Read More

Cancer Control 2013 Apr;20(2):97-110

Radiation Oncology Program, H Lee Moffitt Cancer Center & Research Institute, Tampa, FL 33612, USA.

Background: Squamous cell carcinoma and adenocarcinoma account for more than 90% of all esophageal cancer cases. Although the incidence of squamous cell carcinoma has declined, the incidence of adenocarcinoma has risen due to increases in obesity and gastroesophageal reflux disease.

Methods: The authors examine the role of radiation therapy alone (external beam and brachytherapy) for the management of esophageal cancer or combined with other modalities. Read More

BMC Cancer 2013 Mar 21;13:137. Epub 2013 Mar 21.

Experimental Clinical Research Center at the Max-Delbrueck-Center for Molecular Medicine, Charité Campus Buch, Lindenbergerweg 80, Berlin, 13125, Germany.

Background: We attempted to identify novel biomarkers and therapeutic targets for esophageal squamous cell carcinoma by gene expression profiling of frozen esophageal squamous carcinoma specimens and examined the functional relevance of a newly discovered marker gene, WDR66.

Methods: Laser capture microdissection technique was applied to collect the cells from well-defined tumor areas in collaboration with an experienced pathologist. Whole human gene expression profiling of frozen esophageal squamous carcinoma specimens (n = 10) and normal esophageal squamous tissue (n = 18) was performed using microarray technology. Read More

Oral Dis 2014 Mar 6;20(2):178-85. Epub 2013 Mar 6.

Medicine and Oral Surgery Department, Dental Sciences Group - Health Sciences Research Centre, Higher Institute of Health Sciences (ISCSN), CESPU, Paredes, Portugal.

Objectives: To evaluate the expression of epidermal growth factor receptor (EGFR) and phosphorylated EGFR (pEGFR), in oral squamous cell carcinomas (OSCC). We examined their utility as prognostic markers by relating to clinicopathological characteristics and the clinical outcome.

Materials And Methods: We analysed 74 primary OSCC and examined immunohistochemical expression of EGFR and pEGFR (phosphorylated at tyrosine 1173) using tissue microarray technology. Read More

Thyroid 2013 Nov 19;23(11):1383-9. Epub 2013 Sep 19.

1 Department of Pathology and Laboratory Medicine, University of Pittsburgh Medical Center , Pittsburgh, Pennsylvania.

Background: Follicular variant of papillary thyroid carcinoma (FVPTC) shares features of papillary (PTC) and follicular (FTC) thyroid carcinomas on a clinical, morphological, and genetic level. MicroRNA (miRNA) deregulation was extensively studied in PTCs and FTCs. However, very limited information is available for FVPTC. Read More

J Clin Oncol 2012 Nov 8;30(33):4104-10. Epub 2012 Oct 8.

Molecular Cancer Research, University Medical Center Utrecht, PO Box 85500, 3508 GA Utrecht, the Netherlands.

Purpose: Current assessment of lymph node metastasis in patients with head and neck squamous cell carcinoma is not accurate enough to prevent overtreatment. The aim of this study was validation of a gene expression signature for distinguishing metastasizing (N+) from nonmetastasizing (N0) squamous cell carcinoma of the oral cavity (OSCC) and oropharynx (OPSCC) in a large multicenter cohort, using a diagnostic DNA microarray in a Clinical Laboratory Improvement Amendments/International Organization for Standardization-approved laboratory.

Methods: A multigene signature, previously reported as predictive for the presence of lymph node metastases in OSCC and OPSCC, was first re-evaluated and trained on 94 samples using generic, whole-genome, DNA microarrays. Read More

Eur J Surg Oncol 2013 Jan 25;39(1):17-23. Epub 2012 Jul 25.

Department of Surgery, The Netherlands Cancer Institute - Antoni van Leeuwenhoek Hospital, Plesmanlaan 121, 1066 CX Amsterdam, The Netherlands.

Aim: To evaluate current literature on gene expression profiling in oesophageal cancer.

Methods: We performed a review of the literature (2000-2010) on prognostication and prediction using gene expression analysis in oesophageal cancer.

Results: Seventeen papers comprising 638 patients were included. Read More

J Oral Pathol Med 2012 Oct 4;41(9):667-74. Epub 2012 Apr 4.

Department of Pathology, Forensic Sciences, University Hospital and School of Medicine of Santiago de Compostela, Servicio de Anatomia Patológica, Hospital Clinico de Santiago, Santiago de Compostela, Spain.

Introduction: Carbonic anhydrases (CAs), a group of ubiquitously expressed metalloenzymes, are involved in numerous physiological and pathological processes, including tumorigenicity. Specifically, CA-IX has been primarily found in hypoxic tumor tissues.

Material And Methods: This is a retrospective study of tumors from the Tissue Bank of the Pathology Department of the University Hospital of Santiago de Compostela. Read More

J Oral Pathol Med 2012 Aug 14;41(7):559-67. Epub 2012 Mar 14.

Medicine and Oral Surgery Department, Dental Sciences Group-Health Sciences Research Centre, Instituto Superior de Ciências da Saúde Norte, Paredes, Portugal.

Objective: Our aim was to evaluate the expression of several molecules that regulate growth, the cell cycle and signalling pathways including EGFR, p53, p16 and p27 in oral squamous cell carcinomas (OSCC). We examined their utility as prognostic markers by relating to clinicopathological characteristics and the clinical outcome.

Patients And Methods: Using tissue microarray technology, we analysed 67 primary OSCC and examined immunohistochemical expression of EGFR, p53, p16 and p27. Read More

Pathol Oncol Res 2011 Sep 9;17(3):765-9. Epub 2011 May 9.

Department of Stomatology, First People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.

Recent studies have determined that gene expression profiling using microarray technology can be used to identify tumor-related molecules. The objective of this study was to screen the differentially expressed genes between pleomorphic adenoma (PA) and the normal tissue adjacent to PA using cDNA microarrays and to further validate the differentially expressed genes by real-time PCR. In this study, we selected five pairs of PA and the surrounding normal salivary gland tissues. Read More