J Biotechnol 2019 21;306S:100001. Epub 2018 Dec 21.
Department of Environmental Chemistry and Engineering, Tokyo Institute of Technology, G1-14, Nagatsuta-cho, Midori-ku, Yokohama 226-8502, Japan.
The influence of metal removal with a chelating reagent, ethylenediaminetetraacetic acid (EDTA), and metal reconstitution on the activity of particulate methane monooxygenase (pMMO) from Methylosinus trichosporium OB3b toward methane oxidation to methanol was investigated. For this study, a membrane fraction containing pMMO and bacterial cell membrane components was prepared. pMMO activity was assessed with two different reductants, nicotinamide adenine dinucleotide (NADH) and 2,3,5,6-tetramethyl hydroquinone (duroquinol). Read More