7,624,529 results match your criteria Metabolism: clinical and experimental[Journal]

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[The study of the metabolism of iodotyrosines included in the iodized milk protein in rats].

Vopr Pitan 2018 11;87(3):12-17. Epub 2018 May 11.

The Fund for the Development of Science, Moscow.

In the course of evolution in animals and humans, a complex and effective system for providing the body with iodine in the form of various organic and inorganic compounds was developed. The metabolism of inorganic iodine has been studied quite well, in contrast to the mechanism of assimilation of its organic compounds. Among the latter, iodotyrosines, which are part of iodinated milk proteins, are of particular interest. Read More

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http://dx.doi.org/10.24411/0042-8833-2018-10037DOI Listing

NAD metabolism as a target for metabolic health: have we found the silver bullet?

Diabetologia 2019 Feb 16. Epub 2019 Feb 16.

Department of Nutrition and Movement Sciences, NUTRIM School for Nutrition and Translational Research in Metabolism, Maastricht University, Universiteitssingel 50, P.O. Box 616, 6200 MD, Maastricht, the Netherlands.

NAD has gone in and out of fashion within the scientific community a number of times since its discovery in the early 1900s. Over the last decade, NAD has emerged as a potential target for combatting metabolic disturbances and the mitochondrial dysfunction that is mediated through sirtuin (SIRT) enzymes. The beneficial metabolic effects of the NAD/SIRT axis have triggered an increased interest in NAD as an enhancer of energy metabolism. Read More

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http://dx.doi.org/10.1007/s00125-019-4831-3DOI Listing
February 2019

Omega-3 fatty acid-derived mediators that control inflammation and tissue homeostasis.

Int Immunol 2019 Feb 17. Epub 2019 Feb 17.

Laboratory for Metabolomics, RIKEN Center for Integrative Medical Sciences, Suehiro-cho, Tsurumi-ku, Yokohama, Kanagawa, Japan.

Omega-3 polyunsaturated fatty acids (PUFAs), including eicosapentaenoic acid, docosapentaenoic acid and docosahexaenoic acid, display a wide range of beneficial effects in humans and animals. Many of the biological functions of PUFAs are mediated via bioactive metabolites produced by fatty acid oxygenases such as cyclooxygenases, lipoxygenases and cytochrome P450 monooxygenases. Liquid chromatography-tandem mass spectrometry-based mediator lipidomics revealed a series of novel bioactive lipid mediators derived from omega-3 PUFAs. Read More

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http://dx.doi.org/10.1093/intimm/dxz001DOI Listing
February 2019

Metabolic role of cGMP in S. cerevisiae: The murine phosphodiesterase-5 activity affects yeast cell proliferation by altering the cAMP/cGMP equilibrium.

FEMS Yeast Res 2019 Feb 17. Epub 2019 Feb 17.

Department of Biology and Biotechnology "C. Darwin", Sapienza University of Rome, Piazzale A. Moro 5 00185 Rome, Italy.

In higher eukaryotes cAMP and cGMP are signal molecules of major transduction pathways while phosphodiesterases (PDE) are a superfamily of cAMP/cGMP hydrolysing enzymes, modulatory components of these routes. Saccharomyces cerevisiae harbours two genes for PDE: Pde2 is a high affinity cAMP-hydrolysing enzyme, while Pde1 can hydrolyse both cAMP and cGMP. To gain insight into the metabolic role of cGMP in the physiology of yeast, the murine Pde5a1 gene encoding a specific cGMP-hydrolysing enzyme, was expressed in S. Read More

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http://dx.doi.org/10.1093/femsyr/foz016DOI Listing
February 2019

Update on the impact of type 2 diabetes mellitus on bone metabolism and material properties.

Endocr Connect 2019 Mar;8(3):R55-R70

Department of Medicine III & Center for Healthy Aging, Technische Universität Dresden, Dresden, Germany.

The prevalence of type 2 diabetes mellitus (T2DM) is increasing worldwide, especially as a result of our aging society, high caloric intake and sedentary lifestyle. Besides the well-known complications of T2DM on the cardiovascular system, the eyes, kidneys and nerves, bone strength is also impaired in diabetic patients. Patients with T2DM have a 40-70% increased risk for fractures, despite having a normal to increased bone mineral density, suggesting that other factors besides bone quantity must account for increased bone fragility. Read More

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http://dx.doi.org/10.1530/EC-18-0456DOI Listing

Review: The role of zinc in the endocrine system.

Pak J Pharm Sci 2019 Jan;32(1):231-239

Selcuk University, Medical School, Department of Physiology, Konya, Turkey.

Zinc is essential in the regulation of a variety of physiological and biochemical events in the organism. It plays a critical role in maintaining the cell membrane integrity, protein-carbohydrate-lipid metabolism, immune system, wound injury and in the regulation of a number of other biological processes associated with normal growth and development. Physiological and biochemical levels of many hormones are affected by zinc metabolism. Read More

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January 2019

Statins for primary prevention in adults aged 75 years and older: A nationwide population-based case-control study.

Atherosclerosis 2019 Feb 2;283:28-34. Epub 2019 Feb 2.

Department of Endocrinology and Metabolism, Kyung Hee University Hospital at Gangdong, Kyung Hee University School of Medicine, Seoul, Republic of Korea. Electronic address:

Background And Aims: There is inadequate evidence to establish statin treatment for primary prevention in the elderly. This study evaluated whether statins are beneficial for primary prevention of cardiovascular disease (CVD) and all-cause death in adults aged ≥ 75 years.

Methods: A nationwide, nested case-control study was conducted in Korea. Read More

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http://dx.doi.org/10.1016/j.atherosclerosis.2019.01.030DOI Listing
February 2019

Development of the LC-MS/MS method for determining the p-cresol level in plasma.

J Pharm Biomed Anal 2019 Jan 31;167:149-154. Epub 2019 Jan 31.

Department of Bioanalysis and Drugs Analysis, Faculty of Pharmacy with the Laboratory Medicine Division, Medical University of Warsaw, 1 Banacha Street, 02-097 Warsaw, Poland. Electronic address:

p-Cresol is a protein-bound uremic retention solute that originates in the intestine through bacterial metabolism and accumulates throughout the body in case of kidney failure. To date, there has been no method to analyze unconjugated p-cresol concentration in the blood with a limit of detection lower than 75 pg. Thus, the aim of this study was to develop and validate a novel liquid chromatography-tandem mass spectrometry method for the determination of unconjugated p-cresol in plasma with a lower detection limit than what has been determined using previously described methods. Read More

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http://dx.doi.org/10.1016/j.jpba.2019.01.041DOI Listing
January 2019

Antioxidative enzymes activity and thiol metabolism in three leafy vegetables under Cd stress.

Ecotoxicol Environ Saf 2019 Feb 14;173:214-224. Epub 2019 Feb 14.

Hefei Institutes of Physical Science, Chinese Academy of Sciences, Hefei 230031, China.

The enrichment of Cadmium in vegetables is threatening human health. The study aimed to screen Cd low-enriched leafy vegetables and explore whether antioxidative enzymes and heavy metal chelators are synergistic defensive mechanisms. In this paper, the Cd accumulation and translocation of garland chrysanthemum (Chrysanthemum coronarium L. Read More

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http://dx.doi.org/10.1016/j.ecoenv.2019.02.026DOI Listing
February 2019

Impact of acute sleep restriction on cerebral glucose metabolism during recovery non-rapid eye movement sleep among individuals with primary insomnia and good sleeper controls.

Sleep Med 2019 Jan 4;55:81-91. Epub 2019 Jan 4.

Department of Psychiatry, Center for Sleep and Circadian Science, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA.

Background: Restricting time in bed improves insomnia symptoms, but the neural mechanisms for this effect are unknown. Total and partial acute sleep restriction may be useful paradigms for elucidating these effects. We examined the impact of acute sleep restriction on cerebral glucose metabolism during non-rapid eye movement (NREM) sleep in individuals with primary insomnia (n = 17) and good sleep (n = 19). Read More

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http://dx.doi.org/10.1016/j.sleep.2018.12.007DOI Listing
January 2019

Succession of organics metabolic function of bacterial community in response to addition of earthworm casts and zeolite in maize straw composting.

Bioresour Technol 2019 Feb 4;280:229-238. Epub 2019 Feb 4.

State Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science, Chinese Academy of Sciences, Nanjing 210008, China. Electronic address:

Succession and metabolism functions of bacterial communities were determined in maize straw composting with earthworm casts and zeolite addition by using high-throughput sequencing, Biolog and PICRUSt. Results showed that earthworm casts and zeolite addition increased the temperature, decreased NH contents and affected bacterial community structure. The relative abundances of Firmicutes and Betaproteobacteria increased with earthworm casts and zeolite addition in the late stage. Read More

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http://dx.doi.org/10.1016/j.biortech.2019.02.015DOI Listing
February 2019

Co-metabolic degradation of the antibiotic ciprofloxacin by the enriched bacterial consortium XG and its bacterial community composition.

Sci Total Environ 2019 Feb 4;665:41-51. Epub 2019 Feb 4.

Department of Molecular Biosciences and Bioengineering, University of Hawaii at Manoa, Honolulu, HI 96822, USA.

Ciprofloxacin is a broad spectral and highly refractory antibiotic. It is an emerging pollutant. This study aimed to utilise co-metabolism as a means to degrade ciprofloxacin by a bacterial consortium. Read More

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http://dx.doi.org/10.1016/j.scitotenv.2019.01.322DOI Listing
February 2019

H NMR-based metabolomic analysis of nine organophosphate flame retardants metabolic disturbance in Hep G2 cell line.

Sci Total Environ 2019 Feb 4;665:162-170. Epub 2019 Feb 4.

Key Laboratory of Microbial Technology for Industrial Pollution Control of Zhejiang Province, College of Environment, Zhejiang University of Technology, Hangzhou, Zhejiang 310032, China. Electronic address:

Organophosphate flame retardants (OPFRs) are frequently found in the environment and could be adversely affecting organisms. In fact, nine OPFRs have been shown to cause endocrine disruptions, but information on the metabolism-perturbing properties of these OPFRs remains unclear. In this study, the H-nuclear magnetic resonance (NMR) based metabolomic method was applied to evaluate the metabolic disturbances caused by these nine OPFRs. Read More

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http://dx.doi.org/10.1016/j.scitotenv.2019.02.055DOI Listing
February 2019

Microbial metabolic strategies for overcoming low-oxygen in naturalized freshwater reservoirs surrounding the Athabasca Oil Sands: A proxy for End-Pit Lakes?

Sci Total Environ 2019 Feb 8;665:113-124. Epub 2019 Feb 8.

Great Lakes Institute for Environmental Research, 401 Sunset Ave, University of Windsor, Windsor, Ontario N9B 3P4, Canada.

The success and sustainability of aquatic ecosystems are driven by the complex, cooperative metabolism of microbes. Ecological engineering strategies often strive to harness this syntrophic synergy of microbial metabolism for the reclamation of contaminated environments worldwide. Currently, there is a significant knowledge gap in our understanding of how the natural microbial ecology overcomes thermodynamic limitations in recovering contaminated environments. Read More

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http://dx.doi.org/10.1016/j.scitotenv.2019.02.032DOI Listing
February 2019

Phenanthrene-triggered tricarboxylic acid cycle response in wheat leaf.

Sci Total Environ 2019 Feb 8;665:107-112. Epub 2019 Feb 8.

Stockbridge School of Agriculture, University of Massachusetts, Amherst, MA 01003, USA.

Tricarboxylic acid cycle (TCA cycle) is the most effective energy metabolism pathway in higher plants and animals. However, there is no information about its response in plants under environmental stress, especially under polycyclic aromatic hydrocarbons (PAHs) pollution. Here, this study is the first to discuss the intermediate and related enzyme changes in TCA cycle in plants. Read More

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http://dx.doi.org/10.1016/j.scitotenv.2019.02.119DOI Listing
February 2019

Oxidized alginate hydrogels with the GHK peptide enhance cord blood mesenchymal stem cell osteogenesis: a paradigm for metabolomics-based evaluation of biomaterial design.

Acta Biomater 2019 Feb 14. Epub 2019 Feb 14.

Biological Systems Engineering Laboratory, Department of Chemical Engineering & Chemical Technology, Imperial College London, London, UK. Electronic address:

Oxidized alginate hydrogels are appealing alternatives to natural alginate due to their favourable biodegradability profiles and capacity to self-crosslink with amine containing molecules facilitating functionalization with extracellular matrix cues, which enable modulation of stem cell fate, achieve highly viable 3-D cultures, and promote cell growth. Stem cell metabolism is at the core of cellular fate (proliferation, differentiation, death) and metabolomics provides global metabolic signatures representative of cellular status, being able to accurately identify the quality of stem cell differentiation. Herein, umbilical cord blood mesenchymal stem cells (UCB MSCs) were encapsulated in novel oxidized alginate hydrogels functionalized with the glycine-histidine-lysine (GHK) peptide and differentiated towards the osteoblastic lineage. Read More

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http://dx.doi.org/10.1016/j.actbio.2019.02.017DOI Listing
February 2019

Quantitative label-free mass spectrometry using contralateral and adjacent breast tissues reveal differentially expressed proteins and their predicted impacts on pathways and cellular functions in breast cancer.

J Proteomics 2019 Feb 14. Epub 2019 Feb 14.

Genetics Department, Federal University of Parana, Curitiba, Brazil. Electronic address:

Proteins play an essential role in the biological processes associated with cancer. Their altered expression levels can deregulate critical cellular pathways and interactive networks. In this study, the mass spectrometry-based label-free quantification followed by functional annotation was performed to investigate the most significant deregulated proteins among tissues of primary breast tumor (PT) and axillary metastatic lymph node (LN) and corresponding non-tumor tissues contralateral (NCT) and adjacent (ANT) from patients diagnosed with invasive ductal carcinoma. Read More

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http://dx.doi.org/10.1016/j.jprot.2019.02.007DOI Listing
February 2019

High level of dietary soybean oil affects the glucose and lipid metabolism in large yellow croaker Larimichthys crocea through the insulin-mediated PI3K/AKT signaling pathway.

Comp Biochem Physiol B Biochem Mol Biol 2019 Feb 14. Epub 2019 Feb 14.

The Key Laboratory of Mariculture (Ministry of Education), The Key Laboratory of Aquaculture Nutrition and Feeds (Ministry of Agriculture), Fisheries College, Ocean University of China, Qingdao 266003, China; Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, Wen Hai Road, Qingdao 266237, China.

The present study was conducted to investigate the metabolic responses of glucose and lipid in large yellow croaker Larimichthys crocea (initial weight, 36.80 ± 0.39 g) to high level of dietary soybean oil. Read More

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http://dx.doi.org/10.1016/j.cbpb.2018.12.003DOI Listing
February 2019

Molecular and functional characterization of sirt4 and sirt6 in Megalobrama amblycephala under high glucose metabolism.

Comp Biochem Physiol B Biochem Mol Biol 2019 Feb 14. Epub 2019 Feb 14.

Wuxi Fisheries College, Nanjing Agricultural University, Wuxi 214081, China; Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Ministry of Agriculture, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081, China.

We analyzed the sequences of sirt4 and sirt6 and their changes in expression after oral glucose administration in blunt snout bream (Megalobrama amblycephala). We cloned sirt4 and sirt6 and found that their full-length cDNA sequences were 1530 bp and 1723 bp, respectively; their amino acid sequences were 93% and 92% identical to Danio rerio. Megalobrama amblycephala were fed a high glucose solution (3 g/kg). Read More

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http://dx.doi.org/10.1016/j.cbpb.2019.01.010DOI Listing
February 2019

The expression pattern of calcium signaling-related genes during smoltification of Salmo salar in productive conditions.

Comp Biochem Physiol B Biochem Mol Biol 2019 Feb 14. Epub 2019 Feb 14.

Instituto de Ciencias Marinas y Limnológicas, Universidad Austral de Chile, Valdivia, Chile; Centro Fondap de Investigación de Altas Latitudes (IDEAL), Universidad Austral de Chile, Valdivia, Chile. Electronic address:

Variations in the mRNA expression of hepatic and muscle genes that are related to calcium signaling were analyzed by real-time qPCR in farmed Atlantic salmon (Salmo salar L. 1758) to determine changes in expression between parr and smolt stages. These organs were selected due to their close relationship with calcium signaling and metabolism (e. Read More

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http://dx.doi.org/10.1016/j.cbpb.2019.02.002DOI Listing
February 2019

Inhibitory ASIC2-mediated calcineurin/NFAT against colorectal cancer by triterpenoids extracted from Rhus chinensis Mill.

J Ethnopharmacol 2019 Feb 14. Epub 2019 Feb 14.

Department of Pharmaceutics, Shanghai Eighth People's Hospital, Jiangsu University, Shanghai 200235, China. Electronic address:

Ethnopharmacological Relevance: Studies have shown that the etiology and pathogenesis of colorectal cancer are closely related to the tumor microenvironment, and the cancer tissue is still in the state of "energy deficit" and has to promote energy generation through high glycolysis. Rhus chinensis Mill is a Chinese herbal medicine used to treat various types of solid tumors in China. Colorectal cancer (CRC) is a heterogeneous disease group caused by abnormal changes in glucose metabolism resulted in lactic acid production, which remodels acidosis. Read More

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http://dx.doi.org/10.1016/j.jep.2019.02.029DOI Listing
February 2019

Chemical genetic screen identifies Gapex-5/GAPVD1 and STBD1 as novel AMPK substrates.

Cell Signal 2019 Feb 14. Epub 2019 Feb 14.

Nestlé Institute of Health Sciences SA, Ecole Polytechnique Fédérale de Lausanne (EPFL), Innovation Park, bâtiment G, 1015 Lausanne, Switzerland; School of Life Sciences, EPFL, 1015 Lausanne, Switzerland. Electronic address:

AMP-activated protein kinase (AMPK) is a key regulator of cellular energy homeostasis, acting as a sensor of energy and nutrient status. As such, AMPK is considered a promising drug target for treatment of medical conditions particularly associated with metabolic dysfunctions. To better understand the downstream effectors and physiological consequences of AMPK activation, we have employed a chemical genetic screen in mouse primary hepatocytes in an attempt to identify novel AMPK targets. Read More

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http://dx.doi.org/10.1016/j.cellsig.2019.02.001DOI Listing
February 2019

Control and regulation of the pyrophosphate-dependent glucose metabolism in Entamoeba histolytica.

Mol Biochem Parasitol 2019 Feb 14. Epub 2019 Feb 14.

Departamento de Bioquímica, Instituto Nacional de Cardiología Ignacio Chávez, Ciudad de México, 14080, Mexico.

Entamoeba histolytica has neither Krebs cycle nor oxidative phosphorylation activities; therefore, glycolysis is the main pathway for ATP supply and provision of carbon skeleton precursors for the synthesis of macromolecules. Glucose is metabolized through fermentative glycolysis, producing ethanol as its main end-product as well as some acetate. Amoebal glycolysis markedly differs from the typical Embden-Meyerhof-Parnas pathway present in human cells: (i) by the use of inorganic pyrophosphate, instead of ATP, as the high-energy phospho group donor; (ii) with one exception, the pathway enzymes can catalyze reversible reactions under physiological conditions; (iii) there is no allosteric regulation and sigmoidal kinetic behavior of key enzymes; and (iv) the presence of some glycolytic and fermentation enzymes similar to those of anaerobic bacteria. Read More

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http://dx.doi.org/10.1016/j.molbiopara.2019.02.002DOI Listing
February 2019

Obeticholic acid differentially regulates hepatic injury and inflammation at different stages of D-galactosamine/lipopolysaccharide-evoked acute liver failure.

Eur J Pharmacol 2019 Feb 14. Epub 2019 Feb 14.

Department of Toxicology, Anhui Medical University, Hefei, 230032, China. Electronic address:

The farnesoid X receptor (FXR) is a ligand-activated transcription factor that regulates genes involved in bile acid metabolism. Accumulating data demonstrate that FXR has an anti-inflammatory activity. The present study aimed to investigate the effect of obeticholic acid (OCA), a novel synthetic FXR agonist, on D-galactosamine (GalN)/lipopolysaccharide (LPS)-evoked acute liver injury. Read More

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http://dx.doi.org/10.1016/j.ejphar.2019.02.011DOI Listing
February 2019

Diabetic Hypoglycaemia during Ramadan Fasting: A Trans-National Observational Real-World Study.

Diabetes Res Clin Pract 2019 Feb 14. Epub 2019 Feb 14.

Diabetes Unit, Amiri Hospital, Ministry of Health, Kuwait City, Kuwait. Electronic address:

Objectives: To describe the risk of hypoglycaemia during Ramadan and determine its risk factors, and the impact of hypoglycaemia on patients' behaviour.

Methods: A cross-sectional multi-country observational study, with data captured within 6 weeks after Ramadan 2015. Patients' and disease characteristics and its management, the risk of hypoglycaemia and patients' response to hypoglycaemia were recorded RESULTS: A cohort of 1759 patients; majority with type 2 diabetes mellitus from North Africa, Arabian Gulf, Saudi Arabia, and the Indian subcontinent. Read More

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http://dx.doi.org/10.1016/j.diabres.2019.01.039DOI Listing
February 2019

Extracellular glucose-dependent IPSC enhancement by leptin in fast-spiking to pyramidal neuron connections via JAK2-PI3K pathway in the rat insular cortex.

Neuropharmacology 2019 Feb 14. Epub 2019 Feb 14.

Department of Pharmacology, Nihon University School of Dentistry, 1-8-13 Kanda-Surugadai, Chiyoda-ku, Tokyo, 101-8310, Japan; Division of Oral and Craniomaxillofacial Research, Dental Research Center, Nihon University School of Dentistry, 1-8-13 Kanda-Surugadai, Chiyoda-ku, Tokyo, 101-8310, Japan; Molecular Dynamics Imaging Unit, RIKEN Centre for Life Science Technologies, 6-7-3 Minatojima-minamimachi, Chuo-ku, Kobe, 650-0047, Japan. Electronic address:

Leptin is produced in the adipocytes and plays a pivotal role in regulation of energy balance by controlling appetite and metabolism. Leptin receptors are widely distributed in the brain, especially in the hypothalamus, hippocampus, and neocortex. The insular cortex (IC) processes gustatory and visceral information, which functionally correlate to feeding behavior. Read More

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http://dx.doi.org/10.1016/j.neuropharm.2019.02.021DOI Listing
February 2019

Efficacy of Injection of Freshly Collected Autologous Adipose Tissue Into Perianal Fistulas in Patients With Crohn's Disease.

Gastroenterology 2019 Feb 14. Epub 2019 Feb 14.

Department of Surgery, Pelvic Floor Unit, Aarhus University Hospital, Aarhus, Denmark.

Background And Aims: Perianal fistulas are common in patients with Crohn´s disease (CD). Injections of cultured autologous and allogeneic adipose tissue-derived stem cells have been shown to heal CD-associated fistulas. Unfortunately, this treatment is time-consuming and expensive. Read More

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http://dx.doi.org/10.1053/j.gastro.2019.02.005DOI Listing
February 2019

Nitro-fatty acids protect against steatosis and fibrosis during development of nonalcoholic fatty liver disease in mice.

EBioMedicine 2019 Feb 13. Epub 2019 Feb 13.

Department of Internal Medicine, Michigan Medicine, Ann Arbor, MI, USA. Electronic address:

Background: Nonalcoholic fatty liver disease (NAFLD) and resulting nonalcoholic steatohepatitis (NASH) are reaching global epidemic proportions. Lack of non-invasive diagnostic tools and effective therapies constitute two of the major hurdles for a bona fide treatment and a reversal of NASH progression and/or regression of the disease. Nitro-oleic acid (OA-NO) has been proven effective in multiple experimental models of inflammation and fibrosis. Read More

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http://dx.doi.org/10.1016/j.ebiom.2019.02.019DOI Listing
February 2019

The role of the Hint1 protein in the metabolism of phosphorothioate oligonucleotides drugs and prodrugs, and the release of HS under cellular conditions.

Biochem Pharmacol 2019 Feb 14. Epub 2019 Feb 14.

Department of Bioorganic Chemistry, Centre of Molecular and Macromolecular Studies, Polish Academy of Sciences, Lodz, 91-063, Poland.

Phosphorothioate oligonucleotides (PS-oligos) containing sulfur atom attached in a nonbridging position to the phosphorus atom at one or more internucleotide bond(s) are often used in medicinal applications. Their hydrolysis in cellular media proceeds mainly from the 3'-end, resulting in the appearance of nucleoside 5'-O-phosphorothioates ((d)NMPS), whose further metabolism is poorly understood. We hypothesize that the enzyme responsible for (d)NMPS catabolism could be Hint1, an enzyme that belongs to the histidine triad (HIT) superfamily and is present in all organisms. Read More

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http://dx.doi.org/10.1016/j.bcp.2019.02.018DOI Listing
February 2019

The brown adipose tissue glucagon receptor is functional but not essential for control of energy homeostasis in mice.

Mol Metab 2019 Feb 5. Epub 2019 Feb 5.

Lunenfeld-Tanenbaum Research Institute, Mt. Sinai Hospital, Toronto, Ontario, Canada; Department of Medicine, University of Toronto, Toronto, Ontario, Canada. Electronic address:

Objective: Administration of glucagon (GCG) or GCG-containing co-agonists reduces body weight and increases energy expenditure. These actions appear to be transduced by multiple direct and indirect GCG receptor (GCGR)-dependent mechanisms. Although the canonical GCGR is expressed in brown adipose tissue (BAT) the importance of BAT GCGR activity for the physiological control of body weight, or the response to GCG agonism, has not been defined. Read More

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http://dx.doi.org/10.1016/j.molmet.2019.01.011DOI Listing
February 2019

Pnpla3 silencing with antisense oligonucleotides ameliorates nonalcoholic steatohepatitis and fibrosis in Pnpla3 I148M knock-in mice.

Mol Metab 2019 Feb 5. Epub 2019 Feb 5.

Department of Molecular and Clinical Medicine, University of Gothenburg, Sweden; Clinical Nutrition Unit, Department of Medical and Surgical Sciences, Magna Graecia University, Catanzaro, Italy; Cardiology Department, Sahlgrenska University Hospital, Gothenburg, Sweden. Electronic address:

Objective: Nonalcoholic fatty liver disease (NAFLD) is becoming a leading cause of advanced chronic liver disease. The progression of NAFLD, including nonalcoholic steatohepatitis (NASH), has a strong genetic component, and the most robust contributor is the patatin-like phospholipase domain-containing 3 (PNPLA3) rs738409 encoding the 148M protein sequence variant. We hypothesized that suppressing the expression of the PNPLA3 148M mutant protein would exert a beneficial effect on the entire spectrum of NAFLD. Read More

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http://dx.doi.org/10.1016/j.molmet.2019.01.013DOI Listing
February 2019

Demethylation of the Protein Phosphatase PP2A Promotes Demethylation of Histones to Enable Their Function as a Methyl Group Sink.

Mol Cell 2019 Feb 1. Epub 2019 Feb 1.

Department of Biochemistry, University of Texas Southwestern Medical Center, 5323 Harry Hines Boulevard, Dallas, TX 75390-9038, USA. Electronic address:

Dysregulation of chromatin methylation is associated with defects in cellular differentiation as well as a variety of cancers. How cells regulate the opposing activities of histone methyltransferase and demethylase enzymes to set the methylation status of the epigenome for proper control of gene expression and metabolism remains poorly understood. Here, we show that loss of methylation of the major phosphatase PP2A in response to methionine starvation activates the demethylation of histones through hyperphosphorylation of specific demethylase enzymes. Read More

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http://dx.doi.org/10.1016/j.molcel.2019.01.012DOI Listing
February 2019

Comments on: "A Meta-Analysis of the Association between Microrna-196A2 and Risk of Ischemic Stroke and Coronary Artery Disease in Asian Population".

J Stroke Cerebrovasc Dis 2019 Feb 13. Epub 2019 Feb 13.

Metabolic Disorders Research Center, Endocrinology and Metabolism Molecular-Cellular Sciences Institute, Tehran University of Medical Sciences, Tehran, Iran.

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http://dx.doi.org/10.1016/j.jstrokecerebrovasdis.2019.01.014DOI Listing
February 2019

Change in body mass index from childhood onwards and risk of adult cardiovascular disease.

Trends Cardiovasc Med 2019 Feb 5. Epub 2019 Feb 5.

Center for Clinical Research and Disease Prevention, Bispebjerg and Frederiksberg Hospital, The Capital Region, Copenhagen, Denmark; Novo Nordisk Foundation Center for Basic Metabolic Research, Human Genomics and Metagenomics in Metabolism, University of Copenhagen, Denmark.

Childhood obesity adversely affects the structure and function of the cardiovascular system, but the relationship between excessive weight gain during childhood and adult cardiovascular disease (CVD) is not fully understood. This review summarizes evidence for associations of change in body mass index (BMI) from childhood onwards with CVD outcomes. We found that excessive gain in BMI from childhood onwards was consistently associated with the presence of CVD risk factors, with increased risks of coronary heart disease, and there were suggestions of associations with stroke, atrial fibrillation and heart failure, but a lack of evidence precludes firm conclusions. Read More

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http://dx.doi.org/10.1016/j.tcm.2019.01.011DOI Listing
February 2019

Analysis of the genetic architecture of energy balance and its major determinants dry matter intake and energy-corrected milk yield in primiparous Holstein cows.

J Dairy Sci 2019 Feb 13. Epub 2019 Feb 13.

Department of Animal Sciences, Functional Breeding Group, Georg-August-University Göttingen, D-37077 Göttingen, Germany; Center for Integrated Breeding Research, Georg-August-University Göttingen, D-37077 Göttingen, Germany.

The focus of modern dairy cow breeding programs has shifted from being mainly yield based toward balanced goals that increasingly consider functional traits such as fertility, metabolic stability, and longevity. To improve these traits, a less pronounced energy deficit postpartum is considered a key challenge. On the other hand, feed efficiency and methane emissions are gaining importance, possibly leading to conflicts in the design of breeding goals. Read More

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http://dx.doi.org/10.3168/jds.2018-15480DOI Listing
February 2019

Symposium review: Re-evaluation of National Research Council energy estimates in calf starters.

Authors:
J D Quigley

J Dairy Sci 2019 Feb 13. Epub 2019 Feb 13.

Nurture Research Center, Provimi North America, Cargill Animal Nutrition, Brookville, OH 45309. Electronic address:

Provision of nutrients in appropriate amounts to meet nutrient requirements for growth, production, and reproduction is the basis for modern animal nutrition. Ration formulation systems predict nutrient requirements based on numerous inputs and then predict nutrient supply based on predicted intake and nutrient content of feeds. Energy systems are used to predict energy supply based on gross caloric content of feeds followed by adjustments for digestion and metabolism of ingested energy. Read More

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http://dx.doi.org/10.3168/jds.2018-15367DOI Listing
February 2019

Strategies for glycemic control in nonobese and obese type 2 diabetic patients with coronary artery disease.

Int J Cardiol 2019 Feb 7. Epub 2019 Feb 7.

Department of Diabetes, Endocrinology, and Metabolism, Center Hospital, National Center for Global Health and Medicine, Tokyo, Japan.

Background: This study aimed to assess strategies of insulin-providing (IP) or insulin-sensitizing (IS) therapy for glycemic control in nonobese diabetic patients with coronary artery disease (CAD) with possibly higher cardiovascular risk and lower insulin secretion than obese diabetic patients with CAD.

Methods: We used data from the Bypass Angioplasty Revascularization Investigation 2 Diabetes (BARI 2D) trial to calculate hazard ratio (HR) with 95% confidence interval (95%CI) for outcome events in patients with type 2 diabetes and CAD using Cox proportional hazard models. The comparison between the IP and IS groups was performed using the randomized design of the BARI 2D trial separately for nonobese (n = 1021) and obese (n = 1319) patients. Read More

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http://dx.doi.org/10.1016/j.ijcard.2019.02.008DOI Listing
February 2019

Switchable sniff-cam (gas-imaging system) based on redox reactions of alcohol dehydrogenase for ethanol and acetaldehyde in exhaled breath.

Talanta 2019 May 27;197:249-256. Epub 2018 Dec 27.

Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, 1-5-45 Yushima, Bunkyo-ku, Tokyo 113-8510, Japan; Department of Biomedical Devices and Instrumentation, Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University, 2-3-10 Kanda-Surugadai, Chiyoda-ku, Tokyo 101-0062, Japan. Electronic address:

Measuring the volatile organic compounds (VOCs) released from a human is a promising method for noninvasive disease screening and metabolism assessment. Selectively imaging multiple VOCs derived from human breath and skin gas is expected to improve current gas analysis techniques. In this study, a gas-imaging system (sniff-cam) that can be used to simultaneously image the concentration distribution of multiple VOCs, namely, ethanol (EtOH) and acetaldehyde (AcH), was developed. Read More

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http://dx.doi.org/10.1016/j.talanta.2018.12.070DOI Listing

Simultaneously quantifying intracellular FAD and FMN using a novel strategy of intrinsic fluorescence four-way calibration.

Talanta 2019 May 26;197:105-112. Epub 2018 Dec 26.

State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, Hunan, China. Electronic address:

The simultaneous quantitative analysis of intracellular metabolic coenzymes flavin adenine dinucleotide (FAD) and flavin mononucleotide (FMN) is of interest because they participate in many electron-transfer reactions of metabolism. But, the simultaneous quantitative analysis of FAD and FMN is hard to be achieved by traditional analytical methods. This paper proposes a novel strategy of intrinsic fluorescence coupled with four-way calibration method for simultaneous quantitative analysis of intracellular metabolic coenzymes FAD and FMN. Read More

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http://dx.doi.org/10.1016/j.talanta.2018.12.076DOI Listing

The impacts of warming on the toxicity of carbon nanotubes in mussels.

Mar Environ Res 2019 Jan 29. Epub 2019 Jan 29.

Department of Biology & Center for Environmental and Marine Studies (CESAM), University of Aveiro, 3810-193, Aveiro, Portugal. Electronic address:

With the increased production and research on nanoparticles, the presence of carbon nanotubes (CNTs) in aquatic systems is very likely to increase. Although it has been shown that CNTs may cause toxicity in marine organisms, to our knowledge, the possible impacts under global temperature increase is still unknown. For this reason, biochemical and physiological impacts induced in Mytilus galloprovincialis due to the presence of functionalized multi-walled CNTs (f-MWCNTs) and increased temperature were investigated in the present study. Read More

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http://dx.doi.org/10.1016/j.marenvres.2019.01.013DOI Listing
January 2019

Metabolic rewiring beyond Warburg in chronic lymphocytic leukemia: How much do we actually know?

Crit Rev Oncol Hematol 2019 Feb 19;134:65-70. Epub 2018 Dec 19.

Lady Davis Institute for Medical Research and Segal Cancer Center, Jewish General Hospital, 3755 Cote Ste. Catherine Road, Montreal, Quebec, Canada, H3T 1E2; Department of Oncology, McGill University, Montreal, Quebec, Canada. Electronic address:

Chronic Lymphocytic Leukemia (CLL) is the most common adult leukemia in the western world. CLL consists of the accumulation of malignant B-cells in the blood stream and homing tissues. Although treatable, this disease is not curable, and resistance or relapse is often present. Read More

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http://dx.doi.org/10.1016/j.critrevonc.2018.12.003DOI Listing
February 2019

Antenatal melatonin modulates an enhanced antioxidant/pro-oxidant ratio in pulmonary hypertensive newborn sheep.

Redox Biol 2019 Jan 29;22:101128. Epub 2019 Jan 29.

Programa de Fisiopatología, Instituto de Ciencias Biomédicas (ICBM), Facultad de Medicina, Universidad de Chile, Av. Salvador 486, Providencia 7500922, Santiago, Chile; International Center for Andean Studies (INCAS), Universidad de Chile, Baquedano s/n, Putre, Chile. Electronic address:

Chronic hypobaric hypoxia during fetal and neonatal life induces neonatal pulmonary hypertension. Hypoxia and oxidative stress are driving this condition, which implies an increase generation of reactive oxygen species (ROS) and/or decreased antioxidant capacity. Melatonin has antioxidant properties that decrease oxidative stress and improves pulmonary vascular function when administered postnatally. Read More

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http://dx.doi.org/10.1016/j.redox.2019.101128DOI Listing
January 2019

The influence of temperature on the effects induced by Triclosan and Diclofenac in mussels.

Sci Total Environ 2019 Jan 16;663:992-999. Epub 2019 Jan 16.

Instituto de Ciencias del Mar ICM-CSIC, E-08003 Barcelona, Spain.

Little is known about the consequences of exposure to pharmaceuticals and personal care products (PPCPs) in elevated temperatures associated with climate change. To increase the knowledge on this topic, Mytilus galloprovincialis mussels were exposed to 1.0μg/L of either the antimicrobial Triclosan (TCS) or the anti-inflammatory drug Diclofenac (DIC), at control (17°C) and 4°C raised (21°C) temperatures for 28days. Read More

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http://dx.doi.org/10.1016/j.scitotenv.2019.01.189DOI Listing
January 2019

Dietary supplementation with strawberry induces marked changes in the composition and functional potential of the gut microbiome in diabetic mice.

J Nutr Biochem 2019 Jan 18;66:63-69. Epub 2019 Jan 18.

Department of Nutrition and Integrative Physiology, College of Health, University of Utah, Salt Lake City, UT 84112, USA. Electronic address:

Gut microbiota contributes to the biological activities of berry anthocyanins by transforming them into bioactive metabolites, and anthocyanins support the growth of specific bacteria, indicating a two-way relationship between anthocyanins and microbiota. In the present study, we tested the hypothesis that strawberry supplementation alters gut microbial ecology in diabetic db/db mice. Control (db/+) and diabetic (db/db) mice (7 weeks old) consumed standard diet or diet supplemented with 2. Read More

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http://dx.doi.org/10.1016/j.jnutbio.2019.01.004DOI Listing
January 2019

Unsaturated fatty acids from flaxseed oil and exercise modulate GPR120 but not GPR40 in the liver of obese mice: a new anti-inflammatory approach.

J Nutr Biochem 2018 Dec 12;66:52-62. Epub 2018 Dec 12.

Nutritional Genomics Laboratory, LabGeN, School of Applied Sciences, UNICAMP, São Paulo, Brazil; Nutrigenomics and Lipids Research Center, CELN, School of Applied Sciences, UNICAMP, São Paulo, Brazil. Electronic address:

GPR120 and GPR40 were recently reported as omega-3 (ω3) receptors with anti-inflammatory properties. Physical exercise could increase the expression of these receptors in the liver, improving hepatic metabolism in obesity and type 2 diabetes. Our aim was to investigate GPR120/40 in the liver of lean and obese mice after acute or chronic physical exercise, with or without the supplementation of ω3 rich flaxseed oil (FS), as well as assess the impact of exercise and FS on insulin signaling and inflammation. Read More

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http://dx.doi.org/10.1016/j.jnutbio.2018.12.003DOI Listing
December 2018

SELDI-TOF MS Analysis of Hepatocellular Carcinoma in an Australian Cohort.

J Surg Res 2019 Feb 13;238:127-136. Epub 2019 Feb 13.

Cancer Surgery and Metabolism Research Group, University of Sydney, Kolling Institute of Medical Research, St Leonards, NSW, Australia; Upper GI Surgical Unit, Royal North Shore Hospital and North Shore Private Hospital, St Leonards, NSW, Australia; Discipline of Surgery, The University of Sydney, Camperdown, NSW, Australia.

Background: Hepatocellular carcinoma (HCC) is a common cause of cancer death worldwide. Resection offers the best chance of long-term survival, but a consistent adverse prognostic factor is the presence of microvascular invasion (MVI). In this study, surface-enhanced laser desorption/ionization time-of-flight mass spectrometry (SELDI-TOF MS), a high throughput method of analyzing complex samples, was used to explore differentially expressed proteins between HCC and adjacent nontumour liver tissue (ANLT). Read More

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http://dx.doi.org/10.1016/j.jss.2019.01.008DOI Listing
February 2019

Chronic exposure to microcystin-LR reduces thyroid hormone levels by activating p38/MAPK and MEK/ERK signal pathway.

Ecotoxicol Environ Saf 2019 Feb 13;173:142-148. Epub 2019 Feb 13.

Department of Geriatric Endocrinology, Geriatric Hospital of Nanjing Medical University, Luojia Road 30, Nanjing 210024, China. Electronic address:

Microcystin-LR (MC-LR) is the most toxic and abundant microcystin that produced by cyanobacteria. Previous studies have shown MC-LR had acute toxic to thyroid, however, the mechanism is still unclear, and the effect of long-term, low-dose MC-LR on thyroid remains uncertain. In this study, we investigated the chronic, low-dose effect of MC-LR on mouse thyroid tissues and thyroid hormone metabolism. Read More

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http://dx.doi.org/10.1016/j.ecoenv.2019.02.024DOI Listing
February 2019

Alterations in fingerprints of polychlorinated biphenyls in benthic biota at the Portland Harbor Superfund Site (Oregon, USA) suggest metabolism.

Chemosphere 2019 Feb 11;223:74-82. Epub 2019 Feb 11.

Washington State Department of Ecology, North 4601 Monroe Street, Spokane, WA 99205, USA.

In order to understand the sources and fate of polychlorinated biphenyls (PCBs) in several species of benthic biota, including clams (Corbicula fluminea), oligochaetes (Lumbriculus variegatus), and mussels (Margaritifera falcata and Anodonta nuttalliana) at the Portland Harbor Superfund Site (PHSS), their congener fingerprints were examined. First, diagnostic ratios of congeners known to be metabolizable vs. recalcitrant in the cytochrome P450 (CYP) pathway were significantly lower in biota than in its co-located sediment, indicating metabolism may have occurred. Read More

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http://dx.doi.org/10.1016/j.chemosphere.2019.02.039DOI Listing
February 2019

Metabolic shift of Staphylococcus aureus under sublethal dose of methicillin in the presence of glucose.

J Pharm Biomed Anal 2019 Feb 8;167:140-148. Epub 2019 Feb 8.

Department of Chemistry and Biochemistry, Miami University, Oxford, OH, 45056, USA; Department of Human Sciences, The Ohio State University, Columbus, OH, 43210, USA. Electronic address:

Traditional strategies in developing novel drugs to treat antibiotic-resistant S. aureus have not been very successful to date, therefore, there is an urgent need for creative usage of existing agents that can treat and control S. aureus infection. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S07317085183247
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http://dx.doi.org/10.1016/j.jpba.2019.02.010DOI Listing
February 2019
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H NMR metabolomic analysis of skin and blubber of bottlenose dolphins reveal a functional metabolic dichotomy.

Comp Biochem Physiol Part D Genomics Proteomics 2019 Feb 10;30:25-32. Epub 2019 Feb 10.

Departamento de Recursos del Mar, Cinvestav Unidad Mérida, Mérida, Yucatán 97310, Mexico; CONACYT, Mexico. Electronic address:

The common bottlenose dolphin (Tursiops truncatus) is a carnivorous cetacean thriving in marine environment that is one of the apex predators of the marine food web. They are found in coastal and estuarine ecosystems which are known to be sensitive to environmental impacts. Dolphins are considered sentinel organisms for monitoring the health of coastal marine ecosystems in their role as predators that can bioaccumulate contaminants. Read More

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http://dx.doi.org/10.1016/j.cbd.2019.02.004DOI Listing
February 2019
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