393 results match your criteria Mededelingen Rijksuniversiteit te Gent. Fakulteit van de Landbouwkundige en Toegepaste Biologische Wetenschappen[Journal]


Effect of Propionibacterium acidipropionici on the growth of Yersinia enterocolitica.

Meded Rijksuniv Gent Fak Landbouwkd Toegep Biol Wet 2001 ;66(3b):651-4

Chair of Industrial and Food Microbiology, University Of Warmia and Mazury, Pl. Cieszyński 1, 10-957 Olsztyn, Poland.

The antibacterial effect of Propionibacterium acidipropionici culture on a whey medium was determined in preparations subjected to cell inactivation with UV radiation and chloroform treatment; in concentrated biomass, centrifuged and suspended in sterile water; in a preparation subjected to cell inactivation with chloroform. The test strain was Yersinia enterocolitica 8. The preparations and culture were added, as biopreservatives, to broth media where the test strain was cultured, checking periodically the population development. Read More

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July 2005
2 Reads

Effect of high pressure on the survival rate and antibacterial activity of Bifidobacterium strains.

Meded Rijksuniv Gent Fak Landbouwkd Toegep Biol Wet 2001 ;66(3b):647-50

Chair of Industrial and Food Microbiology, University Of Warmia and Mazury, Pl. Cieszyński 1, 10-957 Olsztyn, Poland.

The effect of pressures of 1000, 700 and 400 MPa/15 min on the survival rate of Bifidobacterium infantis 1/1 was studied. 24 h milk cultures, Garsch cultures, and yogurt--to which centrifuged cell biomass of these bacteria was introduced--were subjected to pressurization. It was found that a pressure of 1000 MPa inactivated populations from 10(7) to 10(9) cfu/ml in all the environments examined. Read More

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July 2005
2 Reads

Influence of vibrations on fat-phase of milk.

Authors:
M Warmińska A Kruk

Meded Rijksuniv Gent Fak Landbouwkd Toegep Biol Wet 2001 ;66(3b):643-6

University of Warmia and Mazury in Olsztyn, Institute of Dairy Science and Technology Development, Heweliusza 1, bl. 31, 10-957 Olsztyn, Poland.

Model studies are presented on the influence of vertical and horizontal vibrations of different frequency (10 to 60 Hz), constant acceleration 1 g, and constant exposition time 60 min on changes in the lipid fraction of milk. It was found that the changes in the examined characteristics of milk fat were dependent on and intensified with the frequency. Read More

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July 2005
4 Reads

Vibrations in milk transportation.

Authors:
M Warmińska A Kruk

Meded Rijksuniv Gent Fak Landbouwkd Toegep Biol Wet 2001 ;66(3b):639-42

University of Warmia and Mazury in Olsztyn, Institute of Dairy Science and Technology Development, Heweliusza 1, bl. 31, 10-957 Olsztyn, Poland.

During transport of milk in three-cell tank truck, the vibrating acceleration ranged generally on the level of 0.5 to 2g (0.5 to 20 m/s2) at the vibrating frequency 2 to 60 Hz. Read More

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July 2005
2 Reads

Extractibility of the protein and peptide fraction for different types of dry fermented sausages in relation to GPC-analysis.

Meded Rijksuniv Gent Fak Landbouwkd Toegep Biol Wet 2001 ;66(3b):635-8

Department of Animal Production, University of Ghent, Melle, Belgium.

The use of GPC (gel filtration chromatography) for investigating the proteolysis during fermentation and ripening of dry fermented sausages (Northern and Mediterranean type) and the choice of a suitable extraction solvent for this technique was evaluated. Solvents in a wide range of acidity were used in this experiment (pH 0.5-13). Read More

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July 2005
3 Reads

Extraction and PCR analysis of soy DNA in chocolate.

Meded Rijksuniv Gent Fak Landbouwkd Toegep Biol Wet 2001 ;66(3b):631-4

AgriFing, Centre for Applied Research and Services, Hogeschool Gent, Voskenslaan 270, B-9000 Gent, Belgium.

The manner in which to discriminate between genetically modified (GM) versus non-modified foodstuffs is based on the presence of newly introduced genes at the protein or DNA level. Current available methods are almost exclusively based on the polymerase chain reaction (PCR). These methods consist of three steps: DNA isolation, amplification of the desired DNA fragment and visualisation of the obtained amplification products. Read More

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July 2005
4 Reads

Assessment of results replicability of staphylococci identification in lactic acid cheese under various diagnostic methods.

Authors:
I Steinka

Meded Rijksuniv Gent Fak Landbouwkd Toegep Biol Wet 2001 ;66(3b):627-30

Department of Commodity and Cargo Science, Gdynia Maritime Academy, Poland.

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July 2005
3 Reads

Synergistic and antagonistic properties of selected cultures of lactic acid and propionic acid bacteria. Part I. Fermentation activity.

Meded Rijksuniv Gent Fak Landbouwkd Toegep Biol Wet 2001 ;66(3b):621-5

Institute of Dairy Science and Technology Development, University of Warmia and Mazury in Olsztyn, Heweliusza 1/207, 10-957 Olsztyn, Poland.

The objective of this work was to study the dynamics of fermentation activity of selected cultures of lactic acid and propionic acid bacteria used in the cheese production. At the same time, it was decided to follow their interactions. The studies were conducted in two model systems with CH-N-19, Nizostar, and their associated cultures in two media. Read More

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July 2005
5 Reads

Synergistic and antagonistic properties of selected cultures of lactic acid and propionic acid bacteria. Part II. Proteolytic activity.

Meded Rijksuniv Gent Fak Landbouwkd Toegep Biol Wet 2001 ;66(3b):617-20

Institute of Dairy Science and Technology Development, University of Warmia and Mazury in Olsztyn, Heweliusza 1 (bl. 31), 10-957 Olsztyn, Poland.

The objective of this work was to study the dynamics of proteolytic activity for selected cultures of propionic acid and lactic acid bacteria used in the rennet cheese production. The studies were carried out in two model systems, with CH-N-19, Nizostar and their associated cultures in two media differing in the nitrogen compound structure. The addition of coagulating enzyme to the medium accelerated the proteolytic processes occurring upon the contribution of the examined cultures but did not influence the changes in the aminopeptidase specificity towards the substrates used (Leu-pNA for LAB and Pro-pNA for PAB). Read More

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July 2005
3 Reads

Activity of adventitious Enterococcus strains on model curdled caprine milk: microbial growth and evolution of concentration of organic acids and lactose throughout time.

Meded Rijksuniv Gent Fak Landbouwkd Toegep Biol Wet 2001 ;66(3b):613-6

Escola Superior de Biotecnologia, Universidade Católica Portuguesa, Rua Dr. António Bernardino de Almeida, P-4200-072 Porto, Portugal.

Enterococci offer a few unique advantages when compared with other lactic acid bacteria in that they are resistant to phages, stable lactose fermenters, tolerant to relatively high concentrations of sodium chloride and optimal growers at relatively high temperatures (Franz et al., 1999). Such genus occurs and grows in a variety of cheeses, especially artisanal cheeses produced in Southern Europe usually from raw milk; they are claimed to play a major role in ripening and aroma development in such cheeses (Freitas et al. Read More

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July 2005
4 Reads

Advances of the WITY system: yeast performance evaluation during the production of sweet wine using Mavrodafni and Carbarnet sauvignon.

Meded Rijksuniv Gent Fak Landbouwkd Toegep Biol Wet 2001 ;66(3b):609-12

Department of Oenology & Spirits Technology, Technological Educational Institute of Athens (TEI), Ag. Spyridona Street, 12210 Egaleo, Athens, Greece.

The production of sweet wine from the Mavrodafni and Cabernet sauvignon varieties was explored. External inoculation with oenological yeast strains was employed. The performance of the two yeast strains were studied under batch and continuous mode and in free and immobilised states. Read More

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July 2005
3 Reads

Evaluation of the antioxidant activity and ascorbic acid content during the room temperature storage of apple subjected to a combined preservation technology.

Meded Rijksuniv Gent Fak Landbouwkd Toegep Biol Wet 2001 ;66(3b):605-8

Institute of Food Science and Technology-CNR, Via Roma 52, 83100 Avellino, Italy.

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July 2005
3 Reads

Use of lactic and propionic acid bacteria in the production of fermented parsley juice.

Meded Rijksuniv Gent Fak Landbouwkd Toegep Biol Wet 2001 ;66(3b):601-4

Chair of Industrial and Food Microbiology, University of Warmia and Mazury, Plac Cieszyński 1, 10-957 Olsztyn, Poland.

Taking into consideration the proliferation rate of Propionibacterium sp. cells, pH changes and sensory properties of the fermented product obtained, selection of propionic acid bacteria (PAB) was made in order to determine their usefulness for the production of fermented parsley juice. The analysis included 12 strains, belonging to the following species: P. Read More

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July 2005
4 Reads

Effect of Lactobacillus plantarum bacteriocin on gram-negative rods.

Meded Rijksuniv Gent Fak Landbouwkd Toegep Biol Wet 2001 ;66(3b):597-600

Chair of Industrial and Food Microbiology, University Of Warmia and Mazury, PI. Cieszyński 1, 10-957 Olsztyn, Poland.

The antibacterial effect of bacteriocins synthesized by Lactobacillus plantarum isolated from fermented carrot juice on Enterobacteriaceae rods coming from food was studied in the experiment. Their antibacterial activity was determined employing the diffusion method, well technique. The results show that the bacteriocins examined were characterized by a different effect on test strains whose development was inhibited at the activity level from 256AU/ml to 4AU/mL. Read More

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July 2005
4 Reads

Influence of temperature and acidity of milk on the degree of transfer of coagulating preparations from milk to the cheese grain.

Meded Rijksuniv Gent Fak Landbouwkd Toegep Biol Wet 2001 ;66(3b):593-6

Institute of Food Biotechnology, University Of Warmia and Mazury, Heewliusz Street 1, 10-759 Olsztyn, Poland.

The effect of temperature, milk acidity and time of curd holding on the degree of passage of enzymic coagulating preparations, was studied. It was found that the degree of the passage of the examined preparations was increasing together with the rise of temperature and milk acidity. Any significant effect of the curd's holding on the quantity of the preparation in the cheese grain was not found. Read More

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July 2005
3 Reads

Survival rate of preserved cultures contained in baker's vaccine.

Meded Rijksuniv Gent Fak Landbouwkd Toegep Biol Wet 2001 ;66(3b):589-92

Chair of Industrial and Food Microbiology, University of Warmia and Mazury in Olsztyn, Plac Cieszyński 1, 10-957 Olsztyn, Poland.

The studies comprised the preparation and preservation, by the method of spray-drying, of baker's vaccine composed of lactic acid bacteria. The selection of particular strains was conducted taking into consideration the fermentation activity and growth dynamics of Lactobacillus rods isolated from plant material. Mixed vaccine was composed of chosen monocultures, characterized by the highest acidifying activity (4 strains). Read More

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July 2005
2 Reads

Identification of whey proteins in tradional Bulgarian yougurt.

Meded Rijksuniv Gent Fak Landbouwkd Toegep Biol Wet 2001 ;66(3b):585-8

Sofia University, Department of General and Industrial Microbiology, 1000 Sofia, Bulgaria.

Functional foods hold a great promise for future trends in human nutrition. Consumption of milk and milk products have a pronounced probiotic effects together with the expected modification of allergenic properties of milk due to the process of fermentation. The proteolytic system of lactic acid bacteria consists a cell wall bound proteinase and several intracellular peptidases, and can contribute to the liberation of bioactive peptides. Read More

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July 2005
2 Reads

Coalescence stability of alpha-lactalbumin-stabilised emulsion.

Authors:
J Leman T Dolgań

Meded Rijksuniv Gent Fak Landbouwkd Toegep Biol Wet 2001 ;66(3b):581-4

Institute of Food Biotechnology, University Of Warmia and Mazury, 10-759 Olsztyn, Poland.

The effects of acidity (pH 5.0 to 7.0) and protein concentration (0. Read More

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July 2005
3 Reads

Effect of environmental factors on the droplet size of o/w emulsion stabilised by beta-lactoglobulin.

Authors:
J Leman T Dolgan

Meded Rijksuniv Gent Fak Landbouwkd Toegep Biol Wet 2001 ;66(3b):577-80

Chair of Food Biotechnology, University of Warmia and Mazury, 10-724 Olsztyn, Poland.

The effect of environmental factors (protein concentration, pH, ionic strength) on emulsification properties of beta-lactoglobulin (beta-Lg) was analysed. The protein was obtained from phospholipoprotein-free whey 25-fold concentrated by ultrafiltration (10 kDa cut off) after precipitation of alpha-lactalbumin (alpha-La) with 6% trichloroacetic acid. The Sauter diameter D(vs) of oil droplets in the emulsion stabilised by beta-Lg was found to increase with increases in the protein concentration in the range of 0. Read More

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July 2005
2 Reads

The sensory properties of gouda-type cheese depending on the degree of paracasein degradation.

Meded Rijksuniv Gent Fak Landbouwkd Toegep Biol Wet 2001 ;66(3b):573-6

University of Warmia and Mazury in Olsztyn, Institute of Dairy Science and Technology Development, Heweliusza 1, 10-957 Olsztyn, Poland.

The proteolysis in cheeses produced with working starter was different than in those produced with concentrate. Different dynamics of proteolysis (N-soluble) and peptolysis (N-amino acid) during ripening resulted in different sensory properties of gouda-type cheeses. Visible differences occurred in eye formation and texture. Read More

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July 2005
4 Reads

Characterisation of lab in typical Salento Pecorino cheese.

Meded Rijksuniv Gent Fak Landbouwkd Toegep Biol Wet 2001 ;66(3b):569-72

Institute of Research for Agriculture and Food Biotechnology, CNR, Via Prov. Le Lecce-Monteroni, 73100 Lecce, Italy.

Twenty-nine strains of Lactic Acid Bacteria isolated from the typical Pecorino cheese of the Salento area of Italy, were identified and grouped according to their genetic similarity. A preliminary characterisation of the strains was conducted by means of morphological and biochemical analysis, but molecular approaches were necessary for the clear identification of the species. For the species detection, the amplification and sequencing of the 16S rDNA gene was employed In addition, restriction analysis of amplified rDNA (ARDRA) and PCR and AFLP fingerprinting enabled inter- and intra-specific variation to be estimated UPGMA cluster analysis was used to divide the strains into distinct clusters which corresponded with the species delineation obtained by molecular identification. Read More

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July 2005
4 Reads

On the enzymatic hydrolysis of whey proteins brought about by cardosin a from Cynara carduncunlus.

Meded Rijksuniv Gent Fak Landbouwkd Toegep Biol Wet 2001 ;66(3b):565-8

Escola Superior de Biotecnologia, Universidade Católica Portuguesa, Rua Dr. António Bernardino de Almeida, 4200-072 Porto, Portugal.

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July 2005
3 Reads

Applications of probiotics.

Authors:
B Degeest

Meded Rijksuniv Gent Fak Landbouwkd Toegep Biol Wet 2001 ;66(3b):557-61

Yakult Belgium NV, Science & PR department, Joseph Wybranlaan 40, B-1070 Brussels, Belgium.

Although not all action mechanisms of probiotics are fully understood, different beneficial health effects related to the consumption of probiotics are proven and accepted by the scientific world. These effects have to be put in the framework of a healthy diet, prevention and an improvement of the general well being. In this short overview, some health-beneficial effects of the regular ingestion of the probiotic strain Lactobacillus casei Shirota by both healthy persons and subjects with specific complaints is reported. Read More

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July 2005
5 Reads

In situ delivery of therapeutic cytokines by genetically engineered Lactococcus lactis.

Authors:
L Steidler

Meded Rijksuniv Gent Fak Landbouwkd Toegep Biol Wet 2001 ;66(3b):551, 553-6

VIS - University of Gent, Dept. Molecular Biology, KL. Ledeganckstraat 35, B-9000 Gent, Belgium.

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July 2005
2 Reads

Antimicrobial potential of probiotic lactic acid bacteria.

Authors:
L Avonts L De Vuyst

Meded Rijksuniv Gent Fak Landbouwkd Toegep Biol Wet 2001 ;66(3b):543-50

Research Group of Industrial Microbiology, Fermentation Technology and Downstream Processing, Department of Applied Biological Sciences, Vrije Universiteit Brussel, B-1050 Brussels, Belgium.

Antibacterial activity is an important characteristic of probiotics. A possible way to achieve this antibacterial activity is through the production of bacteriocins. This study shows that the commercial probiotic strains Lactobacillus johnsonii LA1 and Lactobacillus casei YIT 9029 produce bacteriocins. Read More

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July 2005
6 Reads

A quality analysis of commercial probiotic products.

Meded Rijksuniv Gent Fak Landbouwkd Toegep Biol Wet 2001 ;66(3b):535, 537-42

Laboratory of Microbiology, Ghent University.

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July 2005
2 Reads

Growth of Salmonella in egg white.

Meded Rijksuniv Gent Fak Landbouwkd Toegep Biol Wet 2001 ;66(3b):531-4

Agricultural Research Centre - Ghent, Department of Animal Product Quality and Transformation Technology, Belgium.

Six non-S. Enteritidis strains and eleven S. Enteritidis strains of which 8 were isolated from eggs, were tested on their growth behaviour in fresh eggs and in eggs of 2 and 3 weeks old hold at 20 degrees C. Read More

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July 2005
3 Reads

Inhibitory effects of spices and herbs towards Shigella sonnei and S. flexneri.

Meded Rijksuniv Gent Fak Landbouwkd Toegep Biol Wet 2001 ;66(3b):523-30

Laboratory of Food Microbiology and Food Preservation, Faculty of Agricultural and Applied Biology Sciences, University of Gent, Gent, Belgium.

The use of spices and herbs, their essential oils or their active compounds as means of control of pathogens constitutes an alternative to chemical additives In the present study the antibacterial activities of cloves, thyme, oregano, rosemary and basil on Shigella have been established. Although in a model system in agar media addition of 1% basil could contribute to the 'hurdle' principle and delay outgrowth of Shigella sp. this was not confirmed in real food conditions: the presence of 1% basil did not affect growth of Shigella in potato puree at 22 degrees C or survival at 7 degrees C in spaghetti sauce. Read More

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July 2005
3 Reads

Spore-formers in foods and the food processing chain.

Authors:
M C te Giffel

Meded Rijksuniv Gent Fak Landbouwkd Toegep Biol Wet 2001 ;66(3b):517-22

NIZO food research, Department of Processing, Quality & Safety, PO Box 20, 6710 BA Ede, The Netherlands.

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July 2005
2 Reads

Biosynthesis of exopolysaccharides by two strains of Lactobacillus bulgaricus in whey-based media.

Meded Rijksuniv Gent Fak Landbouwkd Toegep Biol Wet 2001 ;66(3b):511-6

Plovdiv University, Department of Biochemistry & Microbiology, Plovdiv-4000, P.O. Box 1014, Bulgaria.

Exopolysaccharides /EPS/ produced by lactic acid bacteria /LAB/ play a role in the rheology and texture of fermented milks (Cerning 1990) and could also provide a new source of safe additives for use in various food products. The physical properties of EPS, such as rheology and behaviour, depend on several factors, including sugar composition, type of sugar linkages, the presence of organic or inorganic substituents, the degree of polymerization and the length of the side chains. On the other hand certain physiological properties of lactic bacteria are of particular importance in the mechanism of their probiotic functioning: metabolism leading to accumulation of organic acids and other fermentation products in the media; resistance to those metabolites; competitive assimilation of the major nutrients of the media. Read More

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July 2005
2 Reads

Effect of high pressure on some properties of lactic acid bacteria present in kefir.

Meded Rijksuniv Gent Fak Landbouwkd Toegep Biol Wet 2001 ;66(3b):503-9

Institute of Food Biotechnology, University of Warmia and Mazury, Olsztyn, Poland.

The aim of the work was to determine the effect of pressurization under 700 and 1000 MPa on the following: biochemical and antibacterial activity of individual strains of streptococci and lactobacilli, isolated from kefir inoculum, and of kefir microflora. After pressurization under 1000 MPa, the studied microorganisms lost their ability to inhibit the growth of the test strains, preserving the aroma-producing ability at the same time. The pressure of 1000 MPa caused also the changes in conformation of lactic acid, produced by the bacteria in skim milk culture. Read More

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July 2005
3 Reads

Current trends in predictive modelling of microbial lag phenomena.

Meded Rijksuniv Gent Fak Landbouwkd Toegep Biol Wet 2001 ;66(3b):495-502

BioTeC - Bioprocess Technology and Control, Katholieke Universiteit Leuven, Kasteelpark Arenberg 22, B-3001 Heverlee.

This paper summarises recent trends in predictive modelling of microbial lag phenomena. The lag phase is approached from both a qualitative and quantitative point of view. Major modelling approaches and experimental results are critically assessed. Read More

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July 2005
4 Reads

A combination of high oxygen atmosphere and equilibrium modified atmosphere packaging to improve the keeping quality of red fruits.

Meded Rijksuniv Gent Fak Landbouwkd Toegep Biol Wet 2001 ;66(3b):487-94

Ghent University, Department of Food technology and Nutrition, Laboratory of Food Microbiology and Food Preservation, Coupure Links 653, 9000 Gent, Belgium.

When high-barrier material is used to package fresh whole fruit in high oxygen atmospheres, shelf life is limited due to the accumulation of its produced CO2 and its creation of an anaerobic atmosphere. In this study, the effect of the combination of High Oxygen Atmosphere and Equilibrium Modified Atmosphere Packaging was tested on the shelf life of two non-climacteric red fruits, particularly strawberries and raspberries, at 7 degrees C. Herefor, the following packaging systems are compared: the conventional method of packaging in a macroperforated high-barrier film (air conditions) (1), Equilibrium Modified Atmosphere (EMA, i. Read More

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July 2005
4 Reads

The impedimetric technique for quality and hygiene control in the brewery.

Meded Rijksuniv Gent Fak Landbouwkd Toegep Biol Wet 2001 ;66(3b):481-6

Hogeschool Gent, Department Industrial Sciences, Voskenslaan 270, B-9000 Gent, Belgium.

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July 2005
3 Reads

Fish quality assessment with ion mobility based gas detector.

Meded Rijksuniv Gent Fak Landbouwkd Toegep Biol Wet 2001 ;66(3b):475-80

Food and Health Research Centre, University of Kuopio, Box 1627, FIN-70211 Kuopio, Finland.

Measurement of the mixture of extractable volatile compounds of the gills of vendace was used in the fish freshness evaluation (Coregonus albula L.). MGD-1 gas detector was used for the detection of volatile compound profiles of hexane extracts of gills. Read More

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July 2005
4 Reads

Use of cloned DNA fragments as reference materials for event specific quantification of genetically modified organisms (GMOs).

Meded Rijksuniv Gent Fak Landbouwkd Toegep Biol Wet 2001 ;66(3b):469-72

Department for Plant Genetics and Breeding, CLO-Gent, Caritasstraat 21, B-9090 Melle, Belgium.

For the quantification of genetically modified organisms (GMOs) in foods and feeds, real-time PCR is currently the most widely applied technique. To obtain a % of GMO, a GMO-specific target sequence is quantified relatively to a species-specific sequence. The correctness and reliability of the obtained quantitative results fully depend on the reference materials used as standards for setting up external calibration curves. Read More

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July 2005
3 Reads

Effect of a bacterial xylanase and/or antibiotic growth promoter on zootechnical performances of piglets fed arabinoxylan rich diets.

Meded Rijksuniv Gent Fak Landbouwkd Toegep Biol Wet 2001 ;66(3b):467-8

Beldem S.A., rue Bourrie 12, B-5300 Andenne, Belgium.

272 cross bred piglets (Saw (wbp x pbz) x Boar (Hamp x Pietr) were fed a basal diet rich in arabinoxylans from 29 days old to 84 days old. In the prestarter period (29 to 42 d) the basal diet was formulated with 27% wheat, 10% barley, 10% maize, 18% soybean meal and 22% milk products and the analytical content was adjusted at 13.81 MJ/kg metabolisable energy, 19. Read More

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July 2005
2 Reads

Molecular characterisation of Arabidopsis lines tagged with a promoterless gus gene.

Meded Rijksuniv Gent Fak Landbouwkd Toegep Biol Wet 2001 ;66(3b):463-6

Vakgroep Moleculaire Genetica, Departement Plantengenetica, Vlaams Internuniversitair lnstituut voor Biotechnologie (VIB), Universiteit Gent, B-9000 Gent, Belgium.

Promoter trapping has been performed through integration of a T-DNA containing a promoterless beta-glucuronidase (gus) reporter gene into the genome of Arabidopsis thaliana. A collection of T-DNA-tagged Arabidopsis lines has been produced with the vector pdeltagusBin19. Part of this collection was screened for lines with specific gus expression patterns. Read More

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July 2005
1 Read

The aerobic stability of silages influenced by metabolites of lactic acid bacteria.

Meded Rijksuniv Gent Fak Landbouwkd Toegep Biol Wet 2001 ;66(3b):459-61

Institute for Agrobiotechnology Tulln, (IFA-Tulln), Department for Environmental Biotechnology, Konrad Lorenz Str. 20, 3430 Tulln, Austria.

The stability of silages after opening the silo depends strongly on the products formed by the microorganism flora present during the conservation process. A silage additive developed at IFA-Tulln showed best results on different crops. In the starting phase of silage fermentation, acetic acid was formed by the heterofermentative lactic acid bacteria L. Read More

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July 2005
4 Reads

Characterisation of the gastrointestinal bacterial community in pigs fed fermented liquid feed and dry feed: composition and fermentation capacity (phenotypic fingerprint).

Meded Rijksuniv Gent Fak Landbouwkd Toegep Biol Wet 2001 ;66(3b):455-8

Danish Institute of Agricultural Sciences, Department of Animal Nutrition and Physiology, Research Centre Foulum, P.O. Box 50, DK-8830 Tjele, Denmark.

Feeding pigs with fermented liquid feed (FLF) has been shown to reduce the number of enteropathogens such as Salmonella and Brachyospira hyodysenteriae as well as coliform bacteria in general in the gastrointestinal tract (GIT). Also the commensal bacterial populations have been shown to respond to the use of FLF, e.g. Read More

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July 2005
2 Reads

In vitro polyploidy induction in Rhododendron simsii-hybrids.

Meded Rijksuniv Gent Fak Landbouwkd Toegep Biol Wet 2001 ;66(3b):451-4

Department of Plant Genetics and Breeding, Caritasstaat 21, 9090 Melle, Belgium.

Three in vitro protocols for the induction of tetraploid Rhododendron simsii hybrids were evaluated. The most successful technique was a daily application of mitotic poison on the apical meristem of seedlings. However, this technique was rather labour intensive. Read More

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July 2005
2 Reads

Ploidy analysis of azalea flower colour sports.

Meded Rijksuniv Gent Fak Landbouwkd Toegep Biol Wet 2001 ;66(3b):447-9

Department of plant production, University of Gent, Gent, Belgium.

Flower colour variegation is not only a phenomenon of importance to horticulture, the phenotype involved is also often used as a scientific model system for the study of complex gene regulation processes. In the course of such studies on azalea, we observed a correlation between flower colour patterns, flower morphology and somatic polyploidy. Using high-resolution flow cytometry of nuclear DNA, the ploidy level was determined in flowers of different azalea sport families. Read More

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July 2005
3 Reads

Wheat transformation with glutenin gene.

Meded Rijksuniv Gent Fak Landbouwkd Toegep Biol Wet 2001 ;66(3b):443-6

Centre de Recherches agronomiques- Département de Biotechnologie, Chaussée de Charleroi 234, 5030 Gembloux, Belgium.

The rheological properties of wheat grains are associated with the composition of the starchy endosperm in high molecular weight (HMW) glutenin proteins. The HMW glutenin 1xDy12 subunit gene was co introduced with the screenable bar and the reporter gus marker genes in the commercial spring wheat Minaret cultivar (cv). The gene of interest and the marker genes were carried by two separated plasmids, the pBS10BH1 and the pAhC25 respectively. Read More

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July 2005
3 Reads

Characterization of carotenoid biosynthesis in Rhododendron luteum.

Meded Rijksuniv Gent Fak Landbouwkd Toegep Biol Wet 2001 ;66(3b):439-41

Department of Plant Genetics and Breeding, Centre for Agricultural Research-Gent, Caritasstraat 21, B-9090 Melle.

In the color chart of Rhododendron simsii hybrids (Belgian pot azalea), yellow is not yet present, although this color is wanted by growers and consumers. Carotenoid pigments, which are absent in azalea, are responsible for this yellow color of the flower petals. The first objective of this project is characterizing the pathway of carotenoid biosynthesis in the yellow flowers of Rhododendron luteum. Read More

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July 2005
3 Reads

Gene flow from a large scale release of genetically modified herbicide tolerant and hybrid winter oilseed rape (Brassica napus L.).

Meded Rijksuniv Gent Fak Landbouwkd Toegep Biol Wet 2001 ;66(3b):437-8

Department of Plant Genetics and Breeding, Centre for Agricultural Research - Gent., Caristasstraat 21, B-9090 Melle, Belgium.

One of the major concerns regarding the cultivation of transgenic crops is the uncontrolled spread of transgenes among fields or to related wild species. To address this concern a long-term study has been set up to assess the amount of gene flow that is occurring from a large scale field trial of genetically modified herbicide tolerant and hybrid (GMHTH) oilseed rape. During the first year of the project, outcrossing of the herbicide tolerance gene from a large scale release of GMHTH winter oilseed rape to a non-transgenic oilseed rape crop in the vicinity and to wild relatives in the environment has been monitored. Read More

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July 2005
5 Reads

Improvement of sorghum transformation efficiency for increasing nutritional quality.

Meded Rijksuniv Gent Fak Landbouwkd Toegep Biol Wet 2001 ;66(3b):435-6

Laboratory of Plant Genetics, Institute of Molecular Biology and Biotechnology, Vrije Universiteit Brussel, Paadenstraat 65, B-1640 Genesius Rode, Belgium.

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July 2005
3 Reads

Addition of formic acid or starter cultures to liquid feed. Effect on pH, microflora composition, organic acid concentration and ammonia concentration.

Meded Rijksuniv Gent Fak Landbouwkd Toegep Biol Wet 2001 ;66(3b):431-4

Danish Institute of Agricultural Sciences, Department of Animal Nutrition and Physiology, Research Centre Foulum, P.O. Box 50, 8830 Tjele, Denmark.

Some of the charateristics of good quality fermented liquid feed (FLF) are low pH, high numbers of lactic acid bacteria, and low numbers of enterobacteria. In order to test strategies to avoid a proliferation of enterobacteria during the initial phase of FLF elaboration, two in vitro studies were carried out. Addition of various doses of formic acid or two different starter cultures were tested. Read More

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July 2005
2 Reads

The presence of Acetobacter sp. in ensiled forage crops and ensiled industrial byproducts.

Meded Rijksuniv Gent Fak Landbouwkd Toegep Biol Wet 2001 ;66(3b):427-30

ID TNO Animal Nutrition, P.O. Box 65, 8200 AB, Lelystad, The Netherlands.

The presence of acetic acid bacteria (AAB) in whole crop maize silage, whole crop wheat silage, pressed sugar beet pulp silage, grass silage and brewer's grains silage was investigated. AAB could be isolated from whole crop maize silage, whole crop wheat silage and pressed sugar beet pulp silage, but could not be detected in grass silage (> 100 silo's tested) or brewer's grains silage (5 silo's tested). Thirty AAB isolates were characterized to genus level. Read More

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July 2005
3 Reads

Refining of soy bean oil: detection of DNA.

Meded Rijksuniv Gent Fak Landbouwkd Toegep Biol Wet 2001 ;66(3b):417-24

AgriFing, Centre for Applied Research and Services, Hogeschool Gent, Voskenslaan 270, B-9000 Gent, Belgium.

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July 2005
3 Reads

The drl1 gene, a signal identified by transposon tagging is important for leaf growth.

Meded Rijksuniv Gent Fak Landbouwkd Toegep Biol Wet 2001 ;66(3b):409-15

Vakgroep Moleculaire Genetica, Departement Plantengenetica, Vlaams Interuniversitair Instituut voor Biotechnologie (VIB), Universiteit Gent, B-9000 Gent, Belgium.

Control of leaf shape and size was studied at the molecular level by a mutational approach in Arabidopsis thaliana. Mutations that affected leaf growth allowed the identification of genes that are important in that morphological process. The Activator/Dissociation (Ac/Ds) transposable element system of maize was modified to be used as mutagen of the Arabidopsis genome. Read More

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July 2005
2 Reads