3,255 results match your criteria Mechanisms of Development [Journal]


Xenopus slc7a5 is essential for notochord function and eye development.

Mech Dev 2019 02 6;155:48-59. Epub 2019 Jan 6.

Department of Pharmacology and Toxicology, Kyorin University School of Medicine, 6-20-2, Shinkawa, Mitaka, Tokyo 181-8611, Japan. Electronic address:

slc7a5 (also known as LAT1), largely accepted as an amino acid transporter, has been shown to play important roles in cancer and developmental processes. Because knockout mice of Slc7a5 are embryonically lethal due to placental defects, it is difficult to evaluate its role in early development. In this study, expression and function of slc7a5 were evaluated in Xenopus laevis embryos that develop without a placenta. Read More

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http://dx.doi.org/10.1016/j.mod.2019.01.001DOI Listing
February 2019

MoD Special issue on "Developmental Biology in Latin America".

Mech Dev 2018 12;154

Dept Biologia Celular e do Desenvolvimento, Instituto de Ciências Biomédicas, USP, São Paulo, Brasil.

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http://dx.doi.org/10.1016/j.mod.2018.11.003DOI Listing
December 2018

MiR-203a is differentially expressed during branching morphogenesis and EMT in breast progenitor cells and is a repressor of peroxidasin.

Mech Dev 2019 02 1;155:34-47. Epub 2018 Dec 1.

Stem Cell Research Unit, Biomedical Center, Department of Anatomy, Faculty of Medicine, School of Health Sciences, University of Iceland, Iceland; Department of Laboratory Hematology, Landspitali - University Hospital, Iceland. Electronic address:

MicroRNAs regulate developmental events such as branching morphogenesis, epithelial to mesenchymal transition (EMT) and its reverse process mesenchymal to epithelial transition (MET). In this study, we performed small RNA sequencing of a breast epithelial progenitor cell line (D492), and its mesenchymal derivative (D492M) cultured in three-dimensional microenvironment. Among the most downregulated miRNAs in D492M was miR-203a, a miRNA that plays an important role in epithelial differentiation. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S09254773183009
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http://dx.doi.org/10.1016/j.mod.2018.11.002DOI Listing
February 2019
11 Reads

Znfl1s are essential for patterning the anterior-posterior axis of zebrafish posterior hindbrain by acting as direct target genes of retinoic acid.

Mech Dev 2019 02 23;155:27-33. Epub 2018 Nov 23.

Department of Pediatric, Jingjiang People's Hospital Affiliated to Yangzhou University, Jingjiang 214500, China. Electronic address:

RA (retinoic acid) signaling is essential for the patterning the hindbrain of vertebrates. Although hundreds of potential RA targets genes are identified, the ones other than hox genes playing roles in patterning anterior-posterior axis of hindbrain by mediating RA signaling remains largely unknown. Previously, we reported that znfl1s play essential roles in the formation of posterior neuroectoderm in zebrafish embryos. Read More

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http://dx.doi.org/10.1016/j.mod.2018.11.001DOI Listing
February 2019

γ-Tubulin small complex formation is essential for early zebrafish embryogenesis.

Mech Dev 2018 12 3;154:145-152. Epub 2018 Jul 3.

Structural and Molecular Biology Laboratory, Department of Biology, Faculty of Sciences, University of Chile, Santiago, Chile. Electronic address:

The centrosomal protein γ-tubulin is part of the cytoplasmic γ-tubulin small (γ-TuSCs) and large complexes (γ-TuRCs). Both, molecular and cellular evidence indicate that γ-tubulin plays a central role in microtubule nucleation and mitotic spindle formation. However, the molecular mechanisms of complex formation and subsequent biological roles in animal development remain unclear. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S09254773183006
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http://dx.doi.org/10.1016/j.mod.2018.06.006DOI Listing
December 2018
11 Reads

Bone regeneration after traumatic skull injury in Xenopus tropicalis.

Mech Dev 2018 12 2;154:153-161. Epub 2018 Jul 2.

Group for the Study of Developmental Processes (GDeP), Department of Cell Biology, Faculty of Biological Sciences, University of Concepción, Chile; Laboratory of Development and Evolution (LADE), University of Concepción, Chile. Electronic address:

The main purpose of regenerative biology is to improve human health by exploiting cellular and molecular mechanisms favoring tissue repair. In recent years, non-mammalian vertebrates have emerged as powerful model organisms to tackle the problem of tissue regeneration. Here, we analyze the process of bone repair in metamorphosing Xenopus tropicalis tadpoles subjected to traumatic skull injury. Read More

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http://dx.doi.org/10.1016/j.mod.2018.06.007DOI Listing
December 2018

Non-obstructive vas deferens and epididymis loss in cystic fibrosis rats.

Mech Dev 2019 02 2;155:15-26. Epub 2018 Nov 2.

Department of Pediatrics, Emory University School of Medicine, Atlanta, GA, USA. Electronic address:

This study utilizes morphological and mechanistic endpoints to characterize the onset of bilateral atresia of the vas deferens in a recently derived cystic fibrosis (CF) rat model. Embryonic reproductive structures, including Wolffian (mesonephric) duct, Mullerian (paramesonephric) duct, mesonephric tubules, and gonad, were shown to mature normally through late embryogenesis, with involution of the vas deferens and/or epididymis typically occurring between birth and postnatal day 4 (P4), although timing and degree of atresia varied. No evidence of mucus obstruction, which is associated with pathology in other CF-affected tissues, was observed at any embryological or postnatal time point. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S09254773183013
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http://dx.doi.org/10.1016/j.mod.2018.10.002DOI Listing
February 2019
12 Reads

Cochlear connexin 30 homomeric and heteromeric channels exhibit distinct assembly mechanisms.

Mech Dev 2019 02 5;155:8-14. Epub 2018 Oct 5.

GIGA-Neurosciences, Unit of Cell and Tissue Biology, University of Liège, C.H.U. B36, B-4000 Liège, Belgium.

Many of the mutations in GJB2 and GJB6, which encode connexins 26 and 30 (Cx26 and Cx30), impair the formation of membrane channels and cause autosomal syndromic and non-syndromic hearing loss. In cochlear non-sensory supporting cells, Cx26 and Cx30 form two types of homomeric and heteromeric gap junctions. The biogenesis processes of these channels occurring in situ remain largely unknown. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S09254773183014
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http://dx.doi.org/10.1016/j.mod.2018.10.001DOI Listing
February 2019
1 Read

Cog4 is required for protrusion and extension of the epithelium in the developing semicircular canals.

Mech Dev 2019 02 1;155:1-7. Epub 2018 Oct 1.

Institute of Neuroscience, University of Oregon, Eugene, OR 97403, USA. Electronic address:

The semicircular canals in the inner ear sense angular acceleration. In zebrafish, the semicircular canals develop from epithelial projections that grow toward each other and fuse to form pillars. The growth of the epithelial projections is driven by the production and secretion of extracellular matrix components by the epithelium. Read More

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http://dx.doi.org/10.1016/j.mod.2018.09.003DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6226009PMC
February 2019
1 Read

Jitterbug/Filamin and Myosin-II form a complex in tendon cells required to maintain epithelial shape and polarity during musculoskeletal system development.

Mech Dev 2018 12 10;154:309-314. Epub 2018 Sep 10.

Program in Human Genetics, Institute of Biomedical Sciences, Biomedical Neurosciences Institute, Department of Neuroscience, Facultad de Medicina, Universidad de Chile, Santiago, Chile. Electronic address:

During musculoskeletal system development, mechanical tension is generated between muscles and tendon-cells. This tension is required for muscle differentiation and is counterbalanced by tendon-cells avoiding tissue deformation. Both, Jbug/Filamin, an actin-meshwork organizing protein, and non-muscle Myosin-II (Myo-II) are required to maintain the shape and cell orientation of the Drosophila notum epithelium during flight muscle attachment to tendon cells. Read More

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http://dx.doi.org/10.1016/j.mod.2018.09.002DOI Listing
December 2018
1 Read

Evolutionary history of Mo25 gene in plants, a component of RAM/MOR signaling network.

Mech Dev 2018 10 9;153:64-73. Epub 2018 Sep 9.

Evolution and Diversity II Laboratory, Centro de Ciências Naturais e Humanas, Universidade Federal do ABC, Alameda da Universidade, s/n°, São Bernardo do Campo 09606-045, Brazil. Electronic address:

Change in cell morphogenesis is an important feature for proper development of eukaryotes. It is necessary for cell polarity and asymmetry and is essential for asymmetric cell division. RAM/MOR is a conserved signaling network that coordinates cell polarity determinants important for asymmetric cell division and cell polarity establishment. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S09254773183007
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http://dx.doi.org/10.1016/j.mod.2018.09.001DOI Listing
October 2018
5 Reads

Roles of basal keratinocytes in actinotrichia formation.

Mech Dev 2018 10 6;153:54-63. Epub 2018 Sep 6.

Graduate School of Frontier Biosciences, Osaka University, Yamada-oka 1-3, Suita, Osaka 565-0871, Japan. Electronic address:

The embryonic fins and the tip of adult fins of teleost fish are supported by rows of straight, unmineralized fibrils called actinotrichia. The proximal ends of the actinotrichia are bundled and the mineralized bones called lepidotrichia are made along them. Since malformation in actinotrichia causes wavy fin bones, the correct configuration of actinotrichia is essential for the correct construction of the fin shape. Read More

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http://dx.doi.org/10.1016/j.mod.2018.08.010DOI Listing
October 2018

The Period protein homolog LIN-42 regulates germline development in C. elegans.

Mech Dev 2018 10 23;153:42-53. Epub 2018 Aug 23.

Department of Biology, Colgate University, Hamilton, NY 13346, USA. Electronic address:

Germline stem cells are maintained in the distal region of the C. elegans gonad. These cells undergo mitotic divisions, and GLP-1/Notch signaling dictates whether they remain in this state. Read More

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http://dx.doi.org/10.1016/j.mod.2018.08.008DOI Listing
October 2018

Gain-of-function Shh mutants activate Smo cell-autonomously independent of Ptch1/2 function.

Mech Dev 2018 10 23;153:30-41. Epub 2018 Aug 23.

Department of Molecular and Cell Biology, 16 Barker Hall, 3204, University of California, Berkeley, CA 94720, USA. Electronic address:

Sonic Hedgehog (Shh) signaling is characterized by non-cell autonomy; cells expressing Shh do not respond to the ligand. Here, we identify several Shh mutations that can activate the Hedgehog (Hh) pathway cell-autonomously. Cell-autonomous pathway activation requires the extracellular cysteine rich domain of Smoothened, but is otherwise independent of the Shh receptors Patched1 and -2. Read More

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http://dx.doi.org/10.1016/j.mod.2018.08.009DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6165682PMC
October 2018
6 Reads

Fishing forward and reverse: Advances in zebrafish phenomics.

Mech Dev 2018 12 18;154:296-308. Epub 2018 Aug 18.

Department of Cell and Developmental Biology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA. Electronic address:

Understanding how the genome instructs the phenotypic characteristics of an organism is one of the major scientific endeavors of our time. Advances in genetics have progressively deciphered the inheritance, identity and biological relevance of genetically encoded information, contributing to the rise of several, complementary omic disciplines. One of them is phenomics, an emergent area of biology dedicated to the systematic multi-scale analysis of phenotypic traits. Read More

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http://dx.doi.org/10.1016/j.mod.2018.08.007DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6289646PMC
December 2018

Single transcript imaging to assay gene expression in wholemount Drosophila melanogaster tissues.

Mech Dev 2018 10 14;153:10-16. Epub 2018 Aug 14.

TIFR Centre for Interdisciplinary Sciences, Tata Institute of Fundamental Research Hyderabad, 36/P, Gopanpally, Serlingampally Mandal, Hyderabad 500107, Telangana, India. Electronic address:

Single molecule Fluorescence in situ Hybridization (smFISH) for mRNA provides a powerful quantitative handle on expression from endogenous gene loci. While the method has been widely applied in cells in culture, applications to primary tissue samples remain fewer, and often use involved cryosectioning. Even apart from quantitative access to absolute transcript counts in specific tissue volumes, many other advantages of smFISH can be envisaged in tissue samples. Read More

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http://dx.doi.org/10.1016/j.mod.2018.08.006DOI Listing
October 2018
1 Read

Methylation status of the putative Pax6 promoter in olive ridley sea turtle embryos with eye defects: An initial approach.

Mech Dev 2018 12 12;154:287-295. Epub 2018 Aug 12.

Laboratory of Molecular Biology, Centro de Investigación en Alimentación y Desarrollo (CIAD), Avenida Sábalo Cerritos s/n, Mazatlán, Sinaloa 82110, Mexico. Electronic address:

Normal development involves the interplay of genetic and epigenetic regulatory mechanisms. Pax6 is an eye-selector factor responsible for initiating the regulatory cascade for the development of the eyes. For the olive ridley sea turtle (Lepidochelys olivacea), a threatened species, eye malformations have been reported. Read More

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http://dx.doi.org/10.1016/j.mod.2018.08.005DOI Listing
December 2018

An in vitro method for studying subcellular rearrangements during cell polarization in Drosophila melanogaster hemocytes.

Mech Dev 2018 12 7;154:277-286. Epub 2018 Aug 7.

Centro de Regulación del Genoma, Facultad de Ciencias, Universidad de Chile, Santiago 7800024, Chile. Electronic address:

Thanks to the power of Drosophila genetics, this animal model has been a precious tool for scientists to uncover key processes associated to innate immunity. The fly immune system relies on a population of macrophage-like cells, also referred to as hemocytes, which are highly migratory and phagocytic, and can easily be followed in vivo. These cells have shown to play important roles in fly development, both at the embryonic and pupal stages. Read More

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http://dx.doi.org/10.1016/j.mod.2018.08.003DOI Listing
December 2018
1 Read

Exogenous Vitamin D signaling alters skeletal patterning, differentiation, and tissue integration during limb regeneration in the axolotl.

Mech Dev 2018 10 7;153:1-9. Epub 2018 Aug 7.

Department of Biology, University of Massachusetts Boston, Boston, MA, USA. Electronic address:

Urodele amphibians such as the axolotl regenerate complete limbs as adults, and understanding how the "blueprint", or pattern, of the regenerate is established and manipulated are areas of intense interest. Nutrient signaling plays an important role in pattern formation during regeneration. Retinoic acid signaling is the most characterized pathway during this process. Read More

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http://dx.doi.org/10.1016/j.mod.2018.08.004DOI Listing
October 2018
1 Read

Gli2 is required for the induction and migration of Xenopus laevis neural crest.

Mech Dev 2018 12 4;154:219-239. Epub 2018 Aug 4.

Instituto Superior de Investigaciones Biológicas (INSIBIO, CONICET-UNT), San Miguel de Tucumán, Argentina; Instituto de Biología "Dr. Francisco D. Barbieri", Facultad de Bioquímica, Química y Farmacia, Universidad Nacional de Tucumán, San Miguel de Tucumán, Argentina. Electronic address:

The neural crest (NC) is a multipotent migratory embryonic population that is formed during late gastrulation and gives rise to a wide array of derivatives, including cells from the peripheral nervous system (PNS), the craniofacial bones and cartilages, peripheral glial cells, and melanocyte cells, among others. In this work we analyzed the role of the Hedgehog signaling pathway effector gli2 in Xenopus NC. We provide evidence that the gli2 gene is expressed in the prospective, premigratory and migratory NC. Read More

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http://dx.doi.org/10.1016/j.mod.2018.07.010DOI Listing
December 2018
3 Reads

Characterizing the embryonic development of B. hygida (Diptera: Sciaridae) following enzymatic treatment to permeabilize the serosal cuticle.

Mech Dev 2018 12 4;154:270-276. Epub 2018 Aug 4.

Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo, Avenida do Café, sem número, Monte Alegre, 14040-903 Ribeirão Preto, SP, Brazil. Electronic address:

Understanding the evolution of the developmental programs active during dipteran embryogenesis depends on comparative studies. As a counterpoint to the intensively investigated and highly derived cyclorrhaphan flies that include the model organism Drosophila melanogaster, we are studying the basal Diptera Bradysia hygida, a member of the Sciaridae family that is amenable to laboratory cultivation. Here we describe the B. Read More

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http://dx.doi.org/10.1016/j.mod.2018.08.002DOI Listing
December 2018
14 Reads

Latin American contributions to the neural crest field.

Mech Dev 2018 10 4;153:17-29. Epub 2018 Aug 4.

Departamento de Biologia Celular, Embriologia e Genética, Centro de Ciências Biológicas, Universidade Federal de Santa Catarina, Campus Universitário - Trindade, 88040-900 Florianópolis, SC, Brazil. Electronic address:

The neural crest (NC) is one of the most fascinating structures during embryonic development. Unique to vertebrate embryos, these cells give rise to important components of the craniofacial skeleton, such as the jaws and skull, as well as melanocytes and ganglia of the peripheral nervous system. Worldwide, several groups have been studying NC development and specifically in the Latin America (LA) they have been growing in numbers since the 1990s. Read More

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http://dx.doi.org/10.1016/j.mod.2018.07.009DOI Listing
October 2018
1 Read

Formation and dynamics of cytoplasmic domains and their genetic regulation during the zebrafish oocyte-to-embryo transition.

Mech Dev 2018 12 2;154:259-269. Epub 2018 Aug 2.

Department of Biology, Facultad de Ciencias, Universidad de Chile, Santiago, Chile. Electronic address:

Establishment and movement of cytoplasmic domains is of great importance for the emergence of cell polarity, germline segregation, embryonic axis specification and correct sorting of organelles and macromolecules into different embryonic cells. The zebrafish oocyte, egg and zygote are valuable material for the study of cytoplasmic domains formation and dynamics during development. In this review we examined how cytoplasmic domains form and are relocated during zebrafish early embryogenesis. Read More

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http://dx.doi.org/10.1016/j.mod.2018.08.001DOI Listing
December 2018
1 Read

FGF8 and Shh promote the survival and maintenance of multipotent neural crest progenitors.

Mech Dev 2018 12 1;154:251-258. Epub 2018 Aug 1.

Laboratório de Plasticidade e Diferenciação de Células da Crista Neural, Departamento de Biologia Celular, Embriologia e Genética, Centro de Ciências Biológicas, Universidade Federal de Santa Catarina, Campus Universitário - Trindade, 88040-900 Florianópolis, SC, Brazil. Electronic address:

The developmental mechanisms that control the building of the complex head of vertebrates and particularly, facial skeletogenesis, remain poorly known. Progenitor cells derived from the embryonic neural crest (NC) are the major constituents and players of facial tissue development. Deciphering the cellular and molecular machinery that controls NC cell (NCC) differentiation into bone, cartilage, fat and other mesenchymal tissues, is thus a main issue for understanding vertebrate facial variations. Read More

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http://dx.doi.org/10.1016/j.mod.2018.07.012DOI Listing
December 2018

Translating genetic, biochemical and structural information to the calpain view of development.

Mech Dev 2018 12 1;154:240-250. Epub 2018 Aug 1.

Instituto de Ciências Biomédicas, Federal University of Rio de Janeiro and Institute of Molecular Entomology, Brazil.

Embryonic development repeatedly deploys a finite number of signaling pathways to control a multitude of processes such as patterning, growth and differentiation. Diversity in gene expression resulting from these signals depends on the epigenetic landscape as well as the network of interactions between different pathways at a given time. A third mechanism to generate diversity from a sole signal is to modify downstream pathway effectors by modulatory protein activity. Read More

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http://dx.doi.org/10.1016/j.mod.2018.07.011DOI Listing
December 2018

Tracking morphological complexities of organ development in culture.

Mech Dev 2018 12 29;154:179-192. Epub 2018 Jul 29.

Department of Anatomy, School of Medicine, Faculty of Medicine, Pontificia Universidad Católica de Chile, Santiago, Chile. Electronic address:

Organogenesis is one of the most striking process during development. During this period, organ primordia pass throughout several stages in which the level of organisation increases in complexity to achieve the final organ architecture. Organ culture, a method in which an isolated organ is explanted and maintained ex-vivo, is an excellent tool for following the morphological dynamics during development. Read More

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http://dx.doi.org/10.1016/j.mod.2018.07.005DOI Listing
December 2018
2.440 Impact Factor

Tricin enhances osteoblastogenesis through the regulation of Wnt/β-catenin signaling in human mesenchymal stem cells.

Mech Dev 2018 08 2;152:38-43. Epub 2018 Jul 2.

Department of Orthopedic, The Fifth Hospital of Harbin, No. 27 Jiankang Road, Harbin 150040, Heilongjiang, China. Electronic address:

Mesenchymal stem cells (MSCs) are multipotent stromal cells that are capable of differentiate into multilineage cell types including bone in vitro and in vivo.

Aim Of The Study: They have been widely developed as a therapeutic approach for bone tissue repair and regeneration. However, the efficiency of lineage specific differentiation still needs improvement. Read More

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http://dx.doi.org/10.1016/j.mod.2018.07.001DOI Listing
August 2018
2 Reads

Mitochondrial permeability transition pore in sea urchin female gametes.

Mech Dev 2018 12 25;154:208-218. Epub 2018 Jul 25.

Departamento de Biologia Molecular, Universidade Federal da Paraíba, João Pessoa, Paraíba, Brazil. Electronic address:

Mitochondrial permeability transition pore (MPTP) has been associated to calcium homeostasis and reactive oxygen species (ROS) generation in several cell types. While extensively investigated in somatic cells, there are few data regarding MPTP phenomenon in gametes. The aim of the present work was to investigate MPTP occurrence in sea urchin female gametes. Read More

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http://dx.doi.org/10.1016/j.mod.2018.07.008DOI Listing
December 2018
11 Reads

Pdgf1aa regulates zebrafish neural crest cells migration through Hif-1 in an oxygen-independent manner.

Mech Dev 2018 12 18;154:203-207. Epub 2018 Jul 18.

Laboratorio de Biología del Desarrollo, Departamento de Ciencias Biológicas, Facultad de Ciencias de la Vida, Universidad Andrés Bello, Santiago, Chile. Electronic address:

The transcription factor Hif-1α regulates epithelial to mesenchymal transition and neural crest cell chemotaxis in Xenopus. Hif-1α is only stabilised under low oxygen levels, and the in vivo stabilisation of this factor in neural crest cells is poorly understood. Multiple oxygen-independent Hif-1α regulators have been described in cell cultures and cancer models. Read More

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http://dx.doi.org/10.1016/j.mod.2018.07.007DOI Listing
December 2018

Transcriptional cross-regulation of Irre Cell Recognition Module (IRM) members in the Drosophila pupal retina.

Mech Dev 2018 12 18;154:193-202. Epub 2018 Jul 18.

Department of Cellular and Molecular Biology, Ribeirão Preto Medical School, University of São Paulo, Ribeirão Preto, Brazil. Electronic address:

Cell adhesion molecules play a central role in morphogenesis, as they mediate the complex range of interactions between different cell types that result in their arrangement in multicellular organs and tissues. How their coordinated dynamic expression in space and time - an essential requirement for their function - is regulated at the genomic and transcriptional levels constitutes an important, albeit still little understood question. The Irre Cell Recognition Module (IRM) is a highly conserved phylogenetically group of structurally related single pass transmembrane glycoproteins belonging to the immunoglobulin superfamily that in Drosophila melanogaster are encoded by the genes roughest (rst), kin-of-irre (kirre), sticks-and-stones (sns) and hibris (hbs). Read More

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http://dx.doi.org/10.1016/j.mod.2018.07.006DOI Listing
December 2018
1 Read

Ric-8A, a GEF for heterotrimeric G-proteins, controls cranial neural crest cell polarity during migration.

Mech Dev 2018 12 31;154:170-178. Epub 2018 Jul 31.

Laboratory of Signaling and Development (LSD), Chile; Group for the Study of Developmental Processes (GDeP), Department of Biochemistry and Molecular Biology, Faculty of Biological Sciences, University of Concepción, Casilla 160-C, Concepción, Chile. Electronic address:

The neural crest (NC) is a transient embryonic cell population that migrates extensively during development. Ric-8A, a guanine nucleotide exchange factor (GEF) for different Gα subunits regulates cranial NC (CNC) cell migration in Xenopus through a mechanism that still remains to be elucidated. To properly migrate, CNC cells establish an axis of polarization and undergo morphological changes to generate protrusions at the leading edge and retraction of the cell rear. Read More

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http://dx.doi.org/10.1016/j.mod.2018.07.004DOI Listing
December 2018
2 Reads

A catenin-dependent balance between N-cadherin and E-cadherin controls neuroectodermal cell fate choices.

Mech Dev 2018 08 14;152:44-56. Epub 2018 Jul 14.

Division of Biology and Biological Engineering 139-74, California Institute of Technology, Pasadena, CA 91125, United States of America. Electronic address:

Characterizing endogenous protein expression, interaction and function, this study identifies in vivo interactions and competitive balance between N-cadherin and E-cadherin in developing avian (Gallus gallus) neural and neural crest cells. Numerous cadherin proteins, including neural cadherin (Ncad) and epithelial cadherin (Ecad), are expressed in the developing neural plate as well as in neural crest cells as they delaminate from the newly closed neural tube. To clarify independent or coordinate function during development, we examined their expression in the cranial region. Read More

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http://dx.doi.org/10.1016/j.mod.2018.07.003DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6112866PMC
August 2018
2 Reads

Mechanisms of stress-related muscle atrophy in fish: An ex vivo approach.

Mech Dev 2018 12 5;154:162-169. Epub 2018 Jul 5.

Molecular Biology and Tissue Culture Laboratory, Centro de Investigación en Alimentación y Desarrollo, Avenida Sábalo Cerritos s/n, Mazatlán, Sinaloa, Mexico. Electronic address:

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http://dx.doi.org/10.1016/j.mod.2018.07.002DOI Listing
December 2018
15 Reads

Notch signaling in the division of germ layers in bilaterian embryos.

Mech Dev 2018 12 22;154:122-144. Epub 2018 Jun 22.

Universidad de Buenos Aires, Consejo Nacional de Investigaciones Científicas y Técnicas, Instituto de Biología Celular y Neurociencias "Prof. E. De Robertis" (IBCN), Facultad de Medicina, Laboratorio de Embriología Molecular "Prof. Dr. Andrés E. Carrasco", Buenos Aires, Argentina. Electronic address:

Bilaterian embryos are triploblastic organisms which develop three complete germ layers (ectoderm, mesoderm, and endoderm). While the ectoderm develops mainly from the animal hemisphere, there is diversity in the location from where the endoderm and the mesoderm arise in relation to the animal-vegetal axis, ranging from endoderm being specified between the ectoderm and mesoderm in echinoderms, and the mesoderm being specified between the ectoderm and the endoderm in vertebrates. A common feature is that part of the mesoderm segregates from an ancient bipotential endomesodermal domain. Read More

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http://dx.doi.org/10.1016/j.mod.2018.06.005DOI Listing
December 2018

Superoxide dismutase 1 expression is modulated by the core pluripotency transcription factors Oct4, Sox2 and Nanog in embryonic stem cells.

Mech Dev 2018 12 19;154:116-121. Epub 2018 Jun 19.

Laboratorio de Regulación Génica en Células Madre, Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina; Instituto de Química Biológica (IQUIBICEN), Universidad de Buenos Aires - CONICET, Buenos Aires, Argentina; Departamento de Fisiología y Biología Molecular y Celular, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina. Electronic address:

Redox homeostasis is vital for cellular functions and to prevent the detrimental consequences of oxidative stress. Pluripotent stem cells (PSCs) have an enhanced antioxidant system which supports the preservation of their genome. Besides, reactive oxygen species (ROS) are proposed to be involved in both self-renewal maintenance and in differentiation in embryonic stem cells (ESCs). Read More

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http://dx.doi.org/10.1016/j.mod.2018.06.004DOI Listing
December 2018
5 Reads

Anisotropy of cell division and epithelial sheet bending via apical constriction shape the complex folding pattern of beetle horn primordia.

Mech Dev 2018 08 18;152:32-37. Epub 2018 Jun 18.

Graduate School of Bioagricultural Sciences, Nagoya University, Nagoya, Aichi 464-8601, Japan; Faculty of Environmental Earth Sciences, Hokkaido University, Sapporo, Hokkaido 060-0810, Japan. Electronic address:

Insects can dramatically change their outer morphology at molting. To prepare for this drastic transformation, insects generate new external organs as folded primordia under the old cuticle. At molting, these folded primordia are physically extended to form their final outer shape in a very short time. Read More

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http://dx.doi.org/10.1016/j.mod.2018.06.003DOI Listing
August 2018
2 Reads

A role for Lin-28 in growth and metamorphosis in Drosophila melanogaster.

Mech Dev 2018 12 14;154:107-115. Epub 2018 Jun 14.

Departamento de Ciencias Biologicas, Facultad de Ciencias de la Vida, Universidad Andres Bello, Viña del Mar, Chile. Electronic address:

Insect metamorphosis has been a classic model to understand the role of hormones in growth and timing of developmental transitions. In addition to hormones, transitions in some species are regulated by genetic programs, such as the heterochronic gene network discovered in C. elegans. Read More

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http://dx.doi.org/10.1016/j.mod.2018.06.002DOI Listing
December 2018
18 Reads
2.440 Impact Factor

Functional roles of the Ripply-mediated suppression of segmentation gene expression at the anterior presomitic mesoderm in zebrafish.

Mech Dev 2018 08 4;152:21-31. Epub 2018 Jun 4.

Division of Life Science, Graduate School of Science and Engineering, Saitama University, Shimo-Okubo, Sakura-ku, Saitama, Saitama 338-8570, Japan. Electronic address:

Somites sequentially form with a regular interval by the segmentation from the anterior region of the presomitic mesoderm (PSM). The expression of several genes involved in the somite segmentation is switched off at the transition from the anterior PSM to somites. Zebrafish Ripply1, which down-regulates a T-box transcription factor Tbx6, is required for the suppression of segmentation gene expression. Read More

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http://dx.doi.org/10.1016/j.mod.2018.06.001DOI Listing

MicroRNAs and the neural crest: From induction to differentiation.

Mech Dev 2018 12 31;154:98-106. Epub 2018 May 31.

Instituto de Biología Molecular y Celular de Rosario (IBR), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET) - Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario (UNR), Ocampo y Esmeralda, S2000EZP Rosario, Argentina. Electronic address:

MicroRNAs are small noncoding RNAs that can control gene expression by base pairing to partially complementary mRNAs. Regulation by microRNAs plays essential roles in diverse biological processes such as neural crest formation during embryonic development. The neural crest is a multipotent cell population that develops from the dorsal neural fold of vertebrate embryos in order to migrate extensively and differentiate into a variety of tissues. Read More

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http://dx.doi.org/10.1016/j.mod.2018.05.009DOI Listing
December 2018

The neuromuscular junction of Xenopus tadpoles: Revisiting a classical model of early synaptogenesis and regeneration.

Mech Dev 2018 12 26;154:91-97. Epub 2018 May 26.

Neuromuscular Studies Laboratory (NeSt Lab), Center for Advanced Microscopy, Faculty of Biological Sciences, Department of Cell Biology, Universidad de Concepción, Concepción, Chile. Electronic address:

The frog neuromuscular junction (NMJ) has been extensively used as a model system to dissect the mechanisms involved in synapse formation, maturation, maintenance, regeneration, and function. Early NMJ synaptogenesis relies on a combination of cell-autonomous and interdependent pre/postsynaptic communication processes. Due to their transparency, comparatively easy manipulation, and remarkable regenerative abilities, frog tadpoles constitute an excellent model to study NMJ formation and regeneration. Read More

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http://dx.doi.org/10.1016/j.mod.2018.05.008DOI Listing
December 2018
5 Reads

Regulation and function of p53: A perspective from Drosophila studies.

Mech Dev 2018 12 23;154:82-90. Epub 2018 May 23.

Instituto de Agrobiotecnología del Litoral, Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Universidad Nacional del Litoral (UNL), Santa Fe 3000, Argentina; Facultad de Bioquímica y Ciencias Biológicas, Universidad Nacional del Litoral (UNL), Santa Fe 3000, Argentina. Electronic address:

Tp53 is a central regulator of cellular responses to stress and one of the most frequently mutated genes in human cancers. P53 is activated by a myriad of stress signals and drives specific cellular responses depending on stress nature, cell type and cellular context. Additionally to its classical functions in regulating cell cycle arrest, apoptosis and senescence, newly described non-canonical functions of p53 are increasingly coming under the spotlight as important functions not only for its role as a tumour suppressor but also for its non-cancer associated activities. Read More

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http://dx.doi.org/10.1016/j.mod.2018.05.007DOI Listing
December 2018

Distinct roles for the cell adhesion molecule Contactin2 in the development and function of neural circuits in zebrafish.

Mech Dev 2018 08 17;152:1-12. Epub 2018 May 17.

Division of Biological Sciences and Bond Life Sciences Center, University of Missouri, Columbia, MO 65211, USA. Electronic address:

Contactin2 (Cntn2)/Transient Axonal Glycoprotein 1 (Tag1), a neural cell adhesion molecule, has established roles in neuronal migration and axon fasciculation in chick and mouse. In zebrafish, antisense morpholino-based studies have indicated roles for cntn2 in the migration of facial branchiomotor (FBM) neurons, the guidance of the axons of the nucleus of the medial longitudinal fascicle (nucMLF), and the outgrowth of Rohon-Beard (RB) central axons. To study functions of Cntn2 in later stages of neuronal development, we generated cntn2 mutant zebrafish using CRISPR-Cas9. Read More

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http://dx.doi.org/10.1016/j.mod.2018.05.005DOI Listing
August 2018
4 Reads

G-quadruplex in animal development: Contribution to gene expression and genomic heterogeneity.

Mech Dev 2018 12 18;154:64-72. Epub 2018 May 18.

Instituto de Biología Molecular y Celular de Rosario (IBR), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario, Esmeralda y Ocampo, (S2000EZP) Rosario, Santa Fe, Argentina. Electronic address:

During animal development, gene expression is orchestrated by specific and highly evolutionarily conserved mechanisms that take place accurately, both at spatial and temporal levels. The last decades have provided compelling evidence showing that chromatin state plays essential roles in orchestrating most of the stages of development. The DNA molecule can adopt alternative structures different from the helical duplex architecture. Read More

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https://linkinghub.elsevier.com/retrieve/pii/S09254773183000
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http://dx.doi.org/10.1016/j.mod.2018.05.004DOI Listing
December 2018
4 Reads

Fos metamorphoses: Lessons from mutants in model organisms.

Mech Dev 2018 12 5;154:73-81. Epub 2018 Jun 5.

Developmental Neurobiology and Neurophysiology Department, Instituto de Neurobiología, Universidad Nacional Autónoma de México, Campus UNAM Juriquilla, Querétaro c.p.76230, Mexico. Electronic address:

The Fos oncogene gene family is evolutionarily conserved throughout Eukarya. Fos proteins characteristically have a leucine zipper and a basic region with a helix-turn-helix motif that binds DNA. In vertebrates, there are several Fos homologs. Read More

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http://dx.doi.org/10.1016/j.mod.2018.05.006DOI Listing
December 2018
2 Reads

Imaging transcription factors dynamics with advanced fluorescence microscopy methods.

Mech Dev 2018 12 16;154:60-63. Epub 2018 May 16.

Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina; CONICET-Universidad de Buenos Aires, Instituto de Química Biológica (IQUIBICEN), Buenos Aires, Argentina. Electronic address:

Pluripotent stem cells (PSCs) are capable of self-renewing and producing all cell types derived from the three germ layers in response to developmental cues, constituting an important promise for regenerative medicine. Pluripotency depends on specific transcription factors (TFs) that induce genes required to preserve the undifferentiated state and repress other genes related to differentiation. The transcription machinery and regulatory components such as TFs are recruited dynamically on their target genes making it essential exploring their dynamics in living cells to understand the transcriptional output. Read More

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http://dx.doi.org/10.1016/j.mod.2018.05.003DOI Listing
December 2018
1 Read

Genetic interaction between Gli3 and Ezh2 during limb pattern formation.

Mech Dev 2018 06 2;151:30-36. Epub 2018 May 2.

Program in Developmental and Stem Cell Biology, The Hospital for Sick Children, Toronto, ON M5G 0A4, Canada; Department of Molecular Genetics, University of Toronto, Toronto, ON M5S 1A8, Canada; Division of Orthopaedics, The Hospital for Sick Children, University of Toronto, Toronto, ON M5G 1X8, Canada. Electronic address:

Anteroposterior polarity of the early limb bud is essential for proper skeletal pattern formation. In order to establish anterior identity, hedgehog signalling needs to be repressed by GLI3 repressor activity, although the mechanism of repression is not well defined. Here we describe genetic interaction between Gli3 and Enhancer of Zeste 2 (Ezh2) that encodes the histone methyltransferase subunit of Polycomb Repressive Complex 2. Read More

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http://dx.doi.org/10.1016/j.mod.2018.05.002DOI Listing
June 2018
5 Reads

Prmt1 regulates craniofacial bone formation upstream of Msx1.

Mech Dev 2018 08 1;152:13-20. Epub 2018 May 1.

Center for Craniofacial Molecular Biology, Herman Ostrow School of Dentistry, University of Southern California, Los Angeles, CA, USA. Electronic address:

Protein arginine methylation has been recently identified as an important form of post-translational modification (PTM). It is carried out by the protein arginine methyltransferase (PRMT) family of enzymes, which in mammals consists of nine members. Among them, PRMT1 is the major arginine methyltransferase and participates in transcription, signal transduction, development and cancer. Read More

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http://dx.doi.org/10.1016/j.mod.2018.05.001DOI Listing
August 2018
8 Reads

CTCF knockout reveals an essential role for this protein during the zebrafish development.

Mech Dev 2018 12 1;154:51-59. Epub 2018 May 1.

Instituto de Fisiología Celular, Departamento de Genética Molecular, Universidad Nacional Autónoma de México, Ciudad Universitaria, Ciudad de México, Mexico.. Electronic address:

Chromatin regulation and organization are essential processes that regulate gene activity. The CCCTC-binding factor (CTCF) is a protein with different and important molecular functions related with chromatin dynamics. It is conserved since invertebrates to vertebrates, posing it as a factor with an important role in the physiology. Read More

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http://dx.doi.org/10.1016/j.mod.2018.04.006DOI Listing
December 2018
6 Reads

The biology and evolution of the Dilp8-Lgr3 pathway: A relaxin-like pathway coupling tissue growth and developmental timing control.

Mech Dev 2018 12 30;154:44-50. Epub 2018 Apr 30.

Integrative Biomedicine Laboratory, CEDOC, Chronic Diseases Research Center, NOVA Medical School | Faculdade de Ciências Médicas, Universidade Nova de Lisboa, Lisbon, Portugal; Instituto de Investigaciones Bioquímicas de Bahía Blanca (INIBIBB), CONICET and Departamento de Biología, Bioquímica y Farmacia, Universidad Nacional del Sur, Camino La Carrindanga km7, Bahía Blanca, Argentina. Electronic address:

Many insects, like cockroaches, moths, and flies, can regenerate tissues by extending the growth-competent phases of their life cycle. The molecular and cellular players mediating this coordination between tissue growth and developmental timing have been recently discovered in Drosophila. The insulin/relaxin-like peptide, Dilp8, was identified as a factor communicating abnormal growth status of Drosophila larval imaginal discs to the neuroendocrine centers that control the timing of the onset of metamorphosis. Read More

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http://dx.doi.org/10.1016/j.mod.2018.04.005DOI Listing
December 2018
1 Read

Regulation mechanisms and implications of sperm membrane hyperpolarization.

Mech Dev 2018 12 22;154:33-43. Epub 2018 Apr 22.

Laboratory of Cell Signal Transduction Networks, Instituto de Biología Molecular y Celular de Rosario (IBR-CONICET-UNR), Rosario 2000, Argentina; Laboratorio de Especialidades Reproductivas, Facultad de Ciencias Bioquimicas y Farmacéuticas, UNR, Rosario 2000, Argentina. Electronic address:

Mammalian sperm are unable to fertilize the egg immediately after ejaculation. In order to gain fertilization competence, they need to undergo a series of biochemical and physiological modifications inside the female reproductive tract, known as capacitation. Capacitation correlates with two essential events for fertilization: hyperactivation, an asymmetric and vigorous flagellar motility, and the ability to undergo the acrosome reaction. Read More

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http://dx.doi.org/10.1016/j.mod.2018.04.004DOI Listing
December 2018
2 Reads