86,727 results match your criteria Macromolecular rapid communications[Journal]


Structural deciphering of the NG2/CSPG4 proteoglycan multifunctionality.

FASEB J 2018 Dec 14:fj201801670R. Epub 2018 Dec 14.

Centre for Molecular and Translational Oncology (COMT), University of Parma, Parma, Italy.

The chondroitin sulfate proteoglycan 4 ( CSPG4) gene encodes a transmembrane proteoglycan (PG) constituting the largest and most structurally complex macromolecule of the human surfaceome. Its transcript shows an extensive evolutionary conservation and, due to the elaborated intracellular processing of the translated protein, it generates an array of glycoforms with the potential to exert variant-specific functions. CSPG4-mediated molecular events are articulated through the interaction with more than 40 putative ligands and the concurrent involvement of the ectodomain and cytoplasmic tail. Read More

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December 2018

Sugar Concentration and Arrangement on the Surface of Glycopolymer Micelles Affect the Interaction with Cancer Cells.

Biomacromolecules 2018 Dec 14. Epub 2018 Dec 14.

Centre for Advanced Macromolecular Design (CAMD), School of Chemistry , University of New South Wales , Sydney NSW 2052 , Australia.

Glycopolymer-coated nanoparticles have attracted significant interest over the past few years, because of their selective interaction with carbohydrate receptors found on the surface of cells. While the type of carbohydrate determines the strength of the ligand-receptor interaction, the presentation of the sugar can be highly influential as the carbohydrate needs to be accessible in order to display good binding. To shine more light on the relationship between nanoparticle structure and cell uptake, we have designed several micelles based on fructose containing block copolymers, which are selective to GLUT5 receptors found on breast cancer cell lines. Read More

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December 2018

Macromolecular crowding as a means to assess the effectiveness of chondrogenic media.

J Tissue Eng Regen Med 2018 Dec 14. Epub 2018 Dec 14.

Regenerative, Modular & Developmental Engineering Laboratory (REMODEL), Biomedical Sciences Building, National University of Ireland Galway (NUI Galway), Galway, Ireland.

Chondrocyte-based tissue engineering requires in vitro cell expansion, which is associated with phenotypic losses, decrease in collagen II synthesis and increase in collagen I synthesis. Another major obstacle in clinical translation of chondrocyte-based therapies is the lack of extracellular matrix (ECM) in the engineered cartilage substitutes. Various research and commercially available media claim that can maintain chondrogenic phenotype, whilst macromolecular crowding (MMC) has been shown to increase tissue-specific ECM deposition and to maintain cell phenotype in vitro. Read More

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December 2018

Collagen proton fraction from ultrashort echo time magnetization transfer (UTE-MT) MRI modelling correlates significantly with cortical bone porosity measured with micro-computed tomography (μCT).

NMR Biomed 2018 Dec 14:e4045. Epub 2018 Dec 14.

Department of Radiology, University of California, San Diego, CA, USA.

Intracortical bone porosity is a key microstructural parameter that determines bone mechanical properties. While clinical MRI visualizes the cortical bone with a signal void, ultrashort echo time (UTE) MRI can acquire high signal from cortical bone, thus enabling quantitative assessments. Magnetization transfer (MT) imaging combined with UTE-MRI can indirectly assess protons in the bone collagenous matrix, which are inversely related to porosity. Read More

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December 2018

PLGA/PLCA casting and PLGA/PDPA electrospinning bilayer film for prevention of postoperative adhesion.

J Biomed Mater Res B Appl Biomater 2018 Dec 13. Epub 2018 Dec 13.

Sichuan Key Laboratory of Medical Imaging and Department of Radiology, Affiliated Hospital of North Sichuan Medical College, Nanchong, China.

Postoperative adhesion is a common complication and preventing adhesions during or immediately after operation is particularly important. The application of solid barrier materials represents the most successful clinical strategy to prevent postoperative adhesion. However, a simple physical barrier effect might be insufficient in preventing adhesion satisfactorily. Read More

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December 2018

Integrated internal standards: A sample prep-free method for better precision in microchip CE.

Electrophoresis 2018 Dec 13. Epub 2018 Dec 13.

BIOS-Lab on a Chip Group, MESA+ Institute of Nanotechnology, TechMed Centre and Max Planck Center for Complex Fluid Dynamics, University of Twente, Enschede, Overijssel, The Netherlands.

Point-of-care systems based on microchip capillary electrophoresis require single-use, disposable microchips pre-filled with all necessary solutions so an untrained operator only needs to apply the sample and perform the analysis. While microchip fabrication can be (and has been) standardized, some manufacturing differences between microchips are unavoidable. To improve analyte precision without increasing device costs or introducing additional error sources, we recently proposed the use of integrated internal standards (ISTDs): ions added to the BGE in small concentrations which form system peaks in the electropherogram that can be used as a measurement reference. Read More

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December 2018

Generalized Model of the Linear Theory of Electromigration and its Application to Electrokinetic Chromatography: Theory and Software PeakMaster 6 - Next Generation.

Electrophoresis 2018 Dec 13. Epub 2018 Dec 13.

Faculty of Science, Department of Physical and Macromolecular Chemistry, Charles University in Prague, Prague, Czech Republic.

The linear theory of electromigration, including the first-order nonlinear approximation, is generalized to systems with any equilibria fast enough to be considered instantaneous in comparison with the time-scale of peak movement. For example, this theory is practically applied in the electrokinetic chromatography (EKC) mode of the capillary zone electrophoresis. The model enables the calculation of positions and shapes of analyte and system peaks without restricting the number of selectors, the complexation stoichiometry, or simultaneous acid-base equilibria. Read More

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December 2018

Structure and function of Rnt1p: An alternative to RNAi for targeted RNA degradation.

Wiley Interdiscip Rev RNA 2018 Dec 11:e1521. Epub 2018 Dec 11.

Macromolecular Crystallography Laboratory, National Cancer Institute, Frederick, Maryland.

The double-stranded RNA-binding protein (dsRBP) family controls RNA editing, stability, and function in all eukaryotes. The central feature of this family is the recognition of a generic RNA duplex using highly conserved double-stranded RNA-binding domain (dsRBD) that recognizes the characteristic distance between the minor grooves created by the RNA helix. Variations on this theme that confer species and functional specificities have been reported but most dsRBPs retain their capacity to bind generic dsRNA. Read More

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December 2018

Layered calcium phenylphosphonate: a hybrid material for a new generation of nanofillers.

Beilstein J Nanotechnol 2018 20;9:2906-2915. Epub 2018 Nov 20.

SYNPO, akciová společnost, S. K. Neumanna 1316, 532 07 Pardubice, Czech Republic.

The use of nanosheets of layered calcium phenylphosphonate as a filler in a polymeric matrix was investigated. Layered calcium phenylphosphonate (CaPhP), with chemical formula CaCHPO∙2HO, is a hybrid organic-inorganic material that exhibits a hydrophobic character due to the presence of phenyl groups on the surface of the layers. In this paper, various CaPhP synthesis methods were studied with the aim of obtaining a product most suitable for its subsequent exfoliation. Read More

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November 2018
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Characterization of sulfated polysaccharide from Laurencia obtusa and its apoptotic, gastroprotective and antioxidant activities.

Int J Biol Macromol 2018 Dec 10. Epub 2018 Dec 10.

Laboratoire de Développement Chimique, Galénique et Pharmacologique des Médicaments (LR12ES09), Unité de Pharmacologie Marine, Faculté de Pharmacie de Monastir, Université de Monastir, 5000 Monastir, Tunisia. Electronic address:

This study was designed to characterize the physico-chemical properties of the sulfated polysaccharide (SP) isolated from the red alga Laurencia obtusa and to evaluate its apoptotic, gastroprotective and antioxidant activities. The different macromolecular characteristics of SP were determined by size exclusion chromatography combined with multi-angle laser light-scattering detection (SEC-MALLS), Fourier transform infrared spectroscopy (FTIR) analysis and nuclear magnetic resonance spectroscopy (H NMR and C NMR). The native molecular weight of the extracted polysaccharide is high (≥336,900 g·mol). Read More

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December 2018

Triggered disassembly and reassembly of actin networks induces rigidity phase transitions.

Soft Matter 2018 Dec 13. Epub 2018 Dec 13.

Department of Physics and Biophysics, University of San Diego, San Diego, USA.

Non-equilibrium soft materials, such as networks of actin proteins, have been intensely investigated over the past decade due to their promise for designing smart materials and understanding cell mechanics. However, current methods are unable to measure the time-dependent mechanics of such systems or map mechanics to the corresponding dynamic macromolecular properties. Here, we present an experimental approach that combines time-resolved optical tweezers microrheology with diffusion-controlled microfluidics to measure the time-evolution of microscale mechanical properties of dynamic systems during triggered activity. Read More

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December 2018

Bridging the spatiotemporal scales of macromolecular transport in crowded biomimetic systems.

Soft Matter 2018 Dec 13. Epub 2018 Dec 13.

Department of Physics and Biophysics, University of San Diego, 5998 Alcala Park, San Diego, CA 92110, USA.

Crowding plays a key role in the transport and conformations of biological macromolecules. Gene therapy, viral infection, and transfection require DNA to traverse the crowded cytoplasm, including the cytoskeletal network of filamentous proteins. Given the complexity of cellular crowding, the dynamics of biological molecules can be highly dependent on the spatiotemporal scale probed. Read More

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December 2018

Structural characterization and molecular dynamics simulations of the caprine and bovine solute carrier family 11 A1 (SLC11A1).

J Comput Aided Mol Des 2018 Dec 12. Epub 2018 Dec 12.

Section of Cell Biology and Biophysics, Department of Biology, School of Sciences, National and Kapodistrian University of Athens, Panepistimiopolis, 15701, Athens, Greece.

Natural Resistance-Associated Macrophage Proteins are a family of transmembrane divalent metal ion transporters, with important implications in life of both bacteria and mammals. Among them, the Solute Carrier family 11 member A1 (SLC11A1) has been implicated with susceptibility to infection by Mycobacterium avium subspecies paratuberculosis (MAP), potentially causing Crohn's disease in humans and paratuberculosis (PTB) in ruminants. Our previous research had focused on sequencing the mRNA of the caprine slc11a1 gene and pinpointed polymorphisms that contribute to caprine SLC11A1's susceptibility to infection by MAP in PTB. Read More

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December 2018

Design, synthesis, and anticancer activity evaluation of irreversible allosteric inhibitors of the ubiquitin-conjugating enzyme Ube2g2.

Medchemcomm 2018 Nov 20;9(11):1818-1825. Epub 2018 Aug 20.

Macromolecular Crystallography Laboratory , National Cancer Institute , 1050 Boyles Street , Frederick , MD 20702 , USA . Email: ; Email:

The RING finger-dependent ubiquitin ligase (E3) gp78, known as the tumor autocrine motility factor receptor, contributes to tumor progression. The protein interacts with its cognate ubiquitin-conjugating enzyme (E2), Ube2g2, its RING domain and a unique region called G2BR that strongly binds to E2. The binding of G2BR to Ube2g2 allosterically enhances the binding of RING to E2, and the binding of RING triggers the departure of G2BR from E2 also in an allosteric fashion. Read More

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November 2018

Effect of storage and aging conditions on the flexural strength and flexural modulus of CAD/CAM materials.

Dent Mater J 2018 Dec 11. Epub 2018 Dec 11.

Department of Restorative Dentistry, Araraquara Faculty of Dentistry, Sao Paulo State University.

Computer-aided design/computer-aided manufacturing (CAD/CAM) materials were submitted to thermocycling, to identify changes in mechanical behavior. Four CAD/CAM materials were divided in four subordinate groups (n=9): (1) dry out for 7 days, (2) distilled water at 37C for 7 days, (3) 60,000 thermocycles, and (4) 120,000 thermocycles. Following thermocycling, samples were submitted to three-point bending test. Read More

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December 2018

Silicon nanosheets as co-initiators for diaryliodonium induced radical and cationic polymerization.

Nanotechnology 2018 Nov 26. Epub 2018 Nov 26.

Technische Universitat Munchen Fakultat fur Chemie, Garching, 85747, GERMANY.

A system of silicon nanosheets and a diaryliodonium salt was found to initiate cationic and radical polymerizations. The polymerization relies on a syngergistic interaction between the silicon nanomaterial and the diaryliodonium salt, whereby the silicon nanomaterial acts as a co-initiator, inducing the decomposition of the diaryliodonium salt. The decomposition products, in turn, are able to initiate both cationic and radical polymerizations thereby enabling a mild and straightforward reaction procedure to obtain a variety of polymer/nanomaterial composites with cationically and radically polymerizable monomers. Read More

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November 2018

Gas-Phase Dynamics of Collision Induced Unfolding, Collision Induced Dissociation, and Electron Transfer Dissociation-Activated Polymer Ions.

J Am Soc Mass Spectrom 2018 Dec 6. Epub 2018 Dec 6.

Mass Spectrometry Laboratory, MolSys Research unit, Quartier Agora, University of Liège, Allée du Six Aout 11, B-4000, Liège, Belgium.

Polymer characterizations are often performed using mass spectrometry (MS). Aside from MS and different tandem MS (MS/MS) techniques, ion mobility-mass spectrometry (IM-MS) has been recently added to the inventory of characterization technique. However, only few studies have focused on the reproducibility and robustness of polymer IM-MS analyses. Read More

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December 2018

Bacterial FtsZ protein forms phase-separated condensates with its nucleoid-associated inhibitor SlmA.

EMBO Rep 2018 Dec 6. Epub 2018 Dec 6.

Centro de Investigaciones Biológicas, Consejo Superior de Investigaciones Científicas (CSIC), Madrid, Spain

Macromolecular condensation resulting from biologically regulated liquid-liquid phase separation is emerging as a mechanism to organize intracellular space in eukaryotes, with broad implications for cell physiology and pathology. Despite their small size, bacterial cells are also organized by proteins such as FtsZ, a tubulin homolog that assembles into a ring structure precisely at the cell midpoint and is required for cytokinesis. Here, we demonstrate that FtsZ can form crowding-induced condensates, reminiscent of those observed for eukaryotic proteins. Read More

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December 2018

Regulation of Ribosome Biogenesis in Skeletal Muscle Hypertrophy.

Physiology (Bethesda) 2019 Jan;34(1):30-42

The Center for Muscle Biology, College of Health Sciences, University of Kentucky , Lexington, Kentucky.

The ribosome is the enzymatic macromolecular machine responsible for protein synthesis. The rates of protein synthesis are primarily dependent on translational efficiency and capacity. Ribosome biogenesis has emerged as an important regulator of skeletal muscle growth and maintenance by altering the translational capacity of the cell. Read More

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January 2019

Disparity in anomalous diffusion of proteins searching for their target DNA sites in a crowded medium is controlled by the size, shape and mobility of macromolecular crowders.

Soft Matter 2018 Dec 12. Epub 2018 Dec 12.

School of Computational and Integrative Sciences, Jawaharlal Nehru University, New Delhi-110067, India.

Using extensive computer simulations, we analyzed the role of physical properties of molecular crowding agents such as size, shape and mobility in the target search dynamics of DNA binding proteins. Our main result is that the sub-diffusive dynamics of a protein inside a crowded medium strongly depends on the crowder properties and also on the protein's mode of diffusion. For instance, while scanning the DNA one-dimensionally, the protein dynamics does not vary with the change in crowder properties. Read More

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December 2018

Cross 1,3-dipolar cycloadditions of C,N-cyclic azomethine imines with an N-benzyl azomethine ylide: facile access to fused tricyclic 1,2,4-hexahydrotriazines.

Org Biomol Chem 2018 Dec 12. Epub 2018 Dec 12.

College of Chemistry and Chemical Engineering, Xinxiang University, Xinxiang 453000, P. R. China. and MOE Key Laboratory of Macromolecular Synthesis and Functionalization, Zhejiang University, Hangzhou 310058, P. R. China.

A cross 1,3-dipolar cycloaddition of two different ylides between C,N-cyclic azomethine imines with an in situ-generated nonstabilized azomethine ylide from an N-benzyl precursor was realized. The reactions afforded a clean and facile access to diverse fused tricyclic 1,2,4-hexahydrotriazines in high yields (up to 96%). The chemical structures of the typical compounds were confirmed by X-ray single-crystal structure analysis. Read More

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December 2018

Miles to go (mtgo) encodes FNDC3 proteins that interact with the chaperonin subunit CCT3 and are required for NMJ branching and growth in Drosophila.

Dev Biol 2019 Jan 25;445(1):37-53. Epub 2018 Oct 25.

Department of Developmental and Cell Biology, School of Biological Sciences, University of California, Irvine, Irvine, CA 92697-2300, USA. Electronic address:

Analysis of mutants that affect formation and function of the Drosophila larval neuromuscular junction (NMJ) has provided valuable insight into genes required for neuronal branching and synaptic growth. We report that NMJ development in Drosophila requires both the Drosophila ortholog of FNDC3 genes; CG42389 (herein referred to as miles to go; mtgo), and CCT3, which encodes a chaperonin complex subunit. Loss of mtgo function causes late pupal lethality with most animals unable to escape the pupal case, while rare escapers exhibit an ataxic gait and reduced lifespan. Read More

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January 2019

Functional Microdomains in Heart's Pacemaker: A Step Beyond Classical Electrophysiology and Remodeling.

Front Physiol 2018 27;9:1686. Epub 2018 Nov 27.

Department of Medicine, School of Medicine and Public Health, University of Wisconsin-Madison, Madison, WI, United States.

Spontaneous beating of the sinoatrial node (SAN), the primary pacemaker of the heart, is initiated, sustained, and regulated by a complex system that integrates ion channels and transporters on the cell membrane surface (often referred to as "membrane clock") with subcellular calcium handling machinery (by parity of reasoning referred to as an intracellular "Ca clock"). Stable, rhythmic beating of the SAN is ensured by a rigorous synchronization between these two clocks highlighted in the coupled-clock system concept of SAN timekeeping. The emerging results demonstrate that such synchronization of the complex pacemaking machinery at the cellular level depends on tightly regulated spatiotemporal signals which are restricted to precise sub-cellular microdomains and associated with discrete clusters of different ion channels, transporters, and regulatory receptors. Read More

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November 2018

Scalable Manufacturing of Enteric Encapsulation Systems for Site-Specific Oral Insulin Delivery.

Biomacromolecules 2018 Dec 11. Epub 2018 Dec 11.

Oral drug delivery is a more favored mode of administration because of its ease of administration, high patient compliance, and low healthcare costs. However, no oral protein formulations are commercially available currently due to the hostile gastrointestinal (GI) barriers resulting in insignificant oral bioavailability of macromolecular drugs. Herein, we used insulin as a model protein drug, insulin-loaded N-(2-hydroxy)-propyl-3-trimethylammonium chloride modified chitosan (HTCC)/sodium tripolyphosphate (TPP) nanocomplex (NC) as a nanocore was further encapsulated into enteric Eudragit L100-55 material through two-step flash nanocomplexation (FNC) process in a reliable and scalable manner, forming our NC-in-Eudragit composite particles (NE). Read More

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December 2018
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Quantification of the electrostatic and covalent interaction between whey proteins and low methoxyl pectin using pfg-NMR diffusometry.

Magn Reson Chem 2018 Dec 10. Epub 2018 Dec 10.

Particle and Interfacial Technology group, Department of Green Chemistry and Technology, Faculty of Bioscience Engineering, Ghent University, Coupure Links 653, 9000, Gent, Belgium.

As interaction between proteins and polysaccharides is expected to change the average diffusion coefficient and the distribution width, it is believed that NMR diffusometry can be a useful tool to study and quantify the interaction between whey protein isolate (WPI) and low methoxy pectin (LMP). For this purpose, the influence of pH, dry heat treatment duration, and WPI to LMP ratio on WPI and LMP interaction was evaluated using pfg-NMR. At pH 5. Read More

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December 2018
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Soil Type and Cyanobacteria Species Influence the Macromolecular and Chemical Characteristics of the Polysaccharidic Matrix in Induced Biocrusts.

Microb Ecol 2018 Dec 8. Epub 2018 Dec 8.

Department of Agrifood Production and Environmental Sciences (DISPAA), University of Florence, Florence, Italy.

Inoculation of soils with cyanobacteria is proposed as a sustainable biotechnological technique for restoration of degraded areas in drylands due to the important role that cyanobacteria and their exopolysaccharides (EPS) play in the environment. So far, few studies have analyzed the macromolecular and chemical characteristics of the polysaccharidic matrix in induced cyanobacterial biocrusts and the scarce existing studies have mainly focused on sandy soil textures. However, the characteristics of the cyanobacterial polysaccharidic matrix may greatly depend on soil type. Read More

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December 2018

Ubiquitin-Proteasome System Is Required for Efficient Replication of Singapore Grouper Iridovirus.

Front Microbiol 2018 26;9:2798. Epub 2018 Nov 26.

College of Marine Sciences, South China Agricultural University, Guangzhou, China.

The ubiquitin-proteasome system (UPS) serves as the major intracellular pathway for protein degradation and plays crucial roles in several cellular processes. However, little is known about the potential actions of the UPS during fish virus infection. In this study, we elucidated the possible roles of UPS in the life cycle of Singapore grouper iridovirus (SGIV); a large DNA virus that usually causes serious systemic diseases with high mortality in groupers. Read More

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November 2018

Macromolecular-clustered facial amphiphilic antimicrobials.

Nat Commun 2018 12 7;9(1):5231. Epub 2018 Dec 7.

Department of Chemistry and Biochemistry, Columbia, SC, 29208, United States.

Bacterial infections and antibiotic resistance, particularly by Gram-negative pathogens, have become a global healthcare crisis. We report the design of a class of cationic antimicrobial polymers that cluster local facial amphiphilicity from repeating units to enhance interactions with bacterial membranes without requiring a globally conformational arrangement associated with highly unfavorable entropic loss. This concept of macromolecular architectures is demonstrated with a series of multicyclic natural product-based cationic polymers. Read More

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December 2018
10.742 Impact Factor

Multifunctional hybrid 3D network based on hyaluronic acid and a copolymer containing pendant spiroacetal moieties.

Int J Biol Macromol 2018 Dec 5. Epub 2018 Dec 5.

"Gr. T. Popa" University of Medicine and Pharmacy, Iasi, Romania.

The elasticity, soft consistency and similarity to the human body are important characteristics of the gels. Such similarities allow the hydrogels to be biocompatible and, as a consequence, they are very attractive to be applied as biomaterials. This study presents a new strategy for developing a biohybrid complex based on a natural/synthetic polymer conjugate as gel type structure enriched with quercetin, which can be a relevant biomaterial for its antimicrobial properties. Read More

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December 2018

Folding of poly-amino acids and intrinsically disordered proteins in overcrowded milieu induced by pH change.

Int J Biol Macromol 2018 Dec 5. Epub 2018 Dec 5.

Institute of Cytology of the Russian Academy of Sciences, Laboratory of Structural Dynamics, Stability and Folding of Proteins, Tikhoretsky av. 4, St. Petersburg, Russia; Peter the Great St. Petersburg Polytechnic University, Department of Biophysics, Polytechnicheskaya av. 29, St. Petersburg, Russia. Electronic address:

pH-induced structural changes of the synthetic homopolypeptides poly-E, poly-K, poly-R, and intrinsically disordered proteins (IDPs) prothymosin α (ProTα) and linker histone H1, in concentrated PEG solutions simulating macromolecular crowding conditions within the membrane-less organelles, were characterized. The conformational transitions of the studied poly-amino acids in the concentrated PEG solutions depend on the polymerization degree of these homopolypeptides, the size of their side chains, the charge distribution of the side chains, and the crowding agent concentration. The results obtained for poly-amino acids are valid for IDPs having a significant total charge. Read More

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December 2018

Analysis of discrete local variability and structural covariance in macromolecular assemblies using Cryo-EM and focused classification.

Ultramicroscopy 2018 Nov 29. Epub 2018 Nov 29.

Salk Institute for Biological Studies, 10010 N Torrey Pines Rd, La Jolla, CA 92037, USA. Electronic address:

Single-particle electron cryo-microscopy and computational image classification can be used to analyze structural variability in macromolecules and their assemblies. In some cases, a particle may contain different regions that each display a range of distinct conformations. We have developed strategies, implemented within the Frealign and cisTEM image processing packages, to focus-classify on specific regions of a particle and detect potential covariance. Read More

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November 2018

Calcium lactate as an attractive compound to partly replace salt in blue-veined cheese.

J Dairy Sci 2018 Oct 24. Epub 2018 Oct 24.

VetAgro Sup, 89 Avenue de l'Europe, 63370 Lempdes, France; Université Clermont Auvergne, INRA, Mixed Research Unit on Cheese (UMRF), F-15000 Aurillac, France.

In addition to their high sodium content, cheeses are thought to induce an acid load to the body, which is associated with deleterious effects on consumers' health. Our objective was to explore the use of alkalinizing salts in partial substitution of NaCl to reduce both the sodium content and the acid-forming potential of cheese, without altering its sensory properties. Blue-veined cheeses were produced under industrial conditions, using brine salting followed by dry salting with a 4:1 (wt/wt) mixture of calcium lactate:NaCl or calcium citrate: NaCl. Read More

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October 2018

Architectural Dynamics of CaMKII-Actin Networks.

Biophys J 2018 Nov 10. Epub 2018 Nov 10.

The Francis Crick Institute, London, United Kingdom.

Calcium-calmodulin-dependent kinase II (CaMKII) has an important role in dendritic spine remodeling upon synaptic stimulation. Using fluorescence video microscopy and image analysis, we investigated the architectural dynamics of rhodamine-phalloidin stabilized filamentous actin (F-actin) networks cross-linked by CaMKII. We used automated image analysis to identify F-actin bundles and crossover junctions and developed a dimensionless metric to characterize network architecture. Read More

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November 2018

Novel non-nucleotidic STING agonists for cancer immunotherapy.

Future Med Chem 2018 Dec 10. Epub 2018 Dec 10.

Department of Neurosurgery, Harvey Cushing Neuro-Oncology Laboratories, Brigham & Women's Hospital, Harvard Medical School, Boston, MA 02115, USA.

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December 2018

Shear flow simulations of smectic liquid crystals based on the Gay-Berne fluid and the soft sphere string-fluid.

Phys Chem Chem Phys 2018 Dec 6. Epub 2018 Dec 6.

Department of Materials and Environmental Chemistry, Arrhenius Laboratory, Stockholm University, 106 91 Stockholm, Sweden. and Department of Chemistry, Ångström Laboratory, Uppsala University, Box 538 751 21, Uppsala, Sweden and Centre of Advanced Research in Bionanoconjugates and Biopolymers, Petru Poni Institute of Macromolecular Chemistry Aleea Grigore Ghica-Voda, 41A, 700487 Iasi, Romania.

We have studied the shear flow of the smectic A phase of three coarse grained liquid crystal model systems, namely two versions of the Gay-Berne fluid and the soft sphere string-fluid. At low shear rates, the orientation where the smectic layers are parallel to the shear plane and the orientation parallel to the vorticity plane are both stable in all the systems. In one of the Gay-Berne fluids, there is a transition from the orientation parallel to the shear plane to the orientation parallel to the vorticity plane. Read More

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December 2018
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Phytol ameliorated benzo(a)pyrene induced lung carcinogenesis in Swiss albino mice via inhibition of oxidative stress and apoptosis.

Environ Toxicol 2018 Dec 5. Epub 2018 Dec 5.

Department of Biotechnology, Alagappa University (Science Campus), Karaikudi, Tamil Nadu, India.

In the present study, the modulatory effect of phytol against benzo(a)pyrene [B(a)P] induced lung carcinogenesis was investigated in Swiss albino mice. During the experimental period, phytol treatment showed no adverse toxic effect and mortality to the experimental animals. Lung tumor was observed in B(a)P treated group and also in animals post-treated with low concentration (50 mg/kg) of phytol. Read More

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December 2018
3 Reads

Advances in coarse-grained modeling of macromolecular complexes.

Curr Opin Struct Biol 2018 Nov 30;52:119-126. Epub 2018 Nov 30.

Department of Chemistry, The University of Chicago, Chicago, IL, USA. Electronic address:

Recent progress in coarse-grained (CG) molecular modeling and simulation has facilitated an influx of computational studies on biological macromolecules and their complexes. Given the large separation of length-scales and time-scales that dictate macromolecular biophysics, CG modeling and simulation are well-suited to bridge the microscopic and mesoscopic or macroscopic details observed from all-atom molecular simulations and experiments, respectively. In this review, we first summarize recent innovations in the development of CG models, which broadly include structure-based, knowledge-based, and dynamics-based approaches. Read More

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November 2018
2 Reads

Application of combined solid-state NMR and DFT calculations for the study of piracetam polymorphism.

Solid State Nucl Magn Reson 2018 Nov 22;97:17-24. Epub 2018 Nov 22.

Faculty of Pharmacy with the Laboratory Medicine Division, Medical University of Warsaw, Department of Biomaterials Chemistry, Chair of Analytical Chemistry and Biomaterials, Banacha 1, 02-093 Warsaw, Poland.

Piracetam, a popular nootropic drug, widely used in the treatment of age-associated mental decline and disorders of the nervous system such as Alzheimer's disease and dementia exists under normal pressure in three polymorphic forms (P1, P2 and P3) of different stability. In this work the relative stability of piracetam polymorphs depending on the temperature was studied using the ssNMR spectroscopy combined with ab initio DFT calculations. The ssNMR spectroscopy enabled the analysis of polymorphic phase transition in the case of pure active substance as well as polymorphic form identification in the analysis of the commercial solid dosage formulations. Read More

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November 2018
1 Read

Macromolecular crowding tunes protein stability by manipulating solvent accessibility.

Chembiochem 2018 Dec 3. Epub 2018 Dec 3.

Universitat Konstanz, Chemie, Universitaetsstraße 10, 78457, Konstanz, GERMANY.

In all intracellular processes, protein structure and dynamics are subjected to the influence of macromolecular crowding (MC). Here, the impact of different types and sizes of MC agents on the model protein BsCspB are comprehensively investigated under thermal as well as chemical denaturation. We consistently reveal a distinct stabilization of BsCspB in dependence on the MC concentration but not on viscosity, polarity or size of MC agent used. Read More

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December 2018
2 Reads

Multivalent Ligand Binding to Cell Membrane Antigens: Defining the Interplay of Affinity, Valency, and Expression Density.

J Am Chem Soc 2018 Dec 3. Epub 2018 Dec 3.

Nature uses multivalency to govern many biological processes. The development of macromolecular and cellular therapies has largely been dependent on engineering similar polyvalent interactions to enable effective targeting. Such therapeutics typically utilize high-affinity binding domains that have the propensity to recognize both antigen-overexpressing tumors and normal-expressing tissues, leading to "on-target, off-tumor" toxicities. Read More

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December 2018
1 Read

Monitoring Metallo-Macromolecular Assembly Equilibria by Ion Mobility-Mass Spectrometry.

Macromol Rapid Commun 2018 Nov 30:e1800667. Epub 2018 Nov 30.

Departments of Chemistry and Polymer Science, The University of Akron, Akron, OH, 44325, USA.

Ion mobility-mass spectrometry (IM-MS) allows the separation of isomeric and isobaric species on the basis of their size, shape, and charge. The fast separation timescale (ms) and high sensitivity of these measurements make IM-MS an ideally suitable method for monitoring changes in macromolecular structure, such as those occurring in interconverting terpyridine-based metallosupramolecular self-assemblies. IM-MS is used to verify the elemental composition (size) and architecture (shape) of the self-assembled products. Read More

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November 2018

Human RNF113A participates of pre-mRNA splicing in vitro.

J Cell Biochem 2018 Dec 2. Epub 2018 Dec 2.

Department of Cell and Developmental Biology, Institute of Biomedical Sciences, University of São Paulo, São Paulo, Brazil.

Pre-messenger RNA (mRNA) splicing is an essential step in the control of eukaryotic gene expression. During splicing, the introns are removed from the gene transcripts as the exons are ligated to create mature mRNA sequences. Splicing is performed by the spliceosome, which is a macromolecular complex composed of five small nuclear RNAs (snRNAs) and more than 100 proteins. Read More

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December 2018

Biomolecular force fields: where have we been, where are we now, where do we need to go and how do we get there?

J Comput Aided Mol Des 2018 Nov 30. Epub 2018 Nov 30.

Department of Chemistry, University of Massachusetts, Amherst, MA, 01003, USA.

In this perspective, we review the theory and methodology of the derivation of force fields (FFs), and their validity, for molecular simulations, from their inception in the second half of the twentieth century to the improved representations at the end of the century. We examine the representations of the physics embodied in various force fields, their accuracy and deficiencies. The early days in the 1950s and 60s saw FFs first introduced to analyze vibrational spectra. Read More

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November 2018

Original Macromolecular Architectures Based on poly(ε-caprolactone) and poly(ε-thiocaprolactone) Grafted onto Chitosan Backbone.

Int J Mol Sci 2018 Nov 29;19(12). Epub 2018 Nov 29.

BioTeam/ICPEES-ECPM, UMR CNRS 7515, Université de Strasbourg, 25 Rue Becquerel, 67087 Strasbourg CEDEX 2, France.

Polyester and/or polythioester grafted chitosan copolymers were synthesized. For that, poly(ε-caprolactone) (PCL), poly(ε-thiocaprolactone) (PTCL), and their copolymers were first synthesized by ring opening polymerization. Copolymers with caprolactone:thiocaprolactone (CL:TCL) molar ratios of 2:1, 1:1, 1:2 were synthesized. Read More

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November 2018

tRNA Translocation by the Eukaryotic 80S Ribosome and the Impact of GTP Hydrolysis.

Cell Rep 2018 Dec;25(10):2676-2688.e7

Institut für Medizinische Physik und Biophysik, Charité - Universitätsmedizin Berlin, Charitéplatz 1, 10117 Berlin, Germany. Electronic address:

Translocation moves the tRNA⋅mRNA module directionally through the ribosome during the elongation phase of protein synthesis. Although translocation is known to entail large conformational changes within both the ribosome and tRNA substrates, the orchestrated events that ensure the speed and fidelity of this critical aspect of the protein synthesis mechanism have not been fully elucidated. Here, we present three high-resolution structures of intermediates of translocation on the mammalian ribosome where, in contrast to bacteria, ribosomal complexes containing the translocase eEF2 and the complete tRNA⋅mRNA module are trapped by the non-hydrolyzable GTP analog GMPPNP. Read More

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December 2018
2 Reads

Macromolecular crowding tunes 3D collagen architecture and cell morphogenesis.

Biomater Sci 2018 Dec 5. Epub 2018 Dec 5.

Bioengineering, University of California San Diego Jacobs School of Engineering, La Jolla, California, USA.

Collagen I is the primary extracellular matrix component of most solid tumors and influences metastatic progression. Collagen matrix engineering techniques are useful for understanding how this complex biomaterial regulates cancer cell behavior and for improving in vitro cancer models. Here, we establish an approach to tune collagen fibril architecture using PEG as an inert molecular crowding agent during gelation and cell embedding. Read More

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December 2018
1 Read

The dynamical interplay between a megadalton peptide nanocage and solutes probed by microsecond atomistic MD; implications for design.

Phys Chem Chem Phys 2018 Dec 5. Epub 2018 Dec 5.

School of Biochemistry, Biomedical Sciences Building, University Walk, Bristol, BS8 1TD, UK. and BrisSynBio, Life Sciences Building, Tyndall Avenue, Bristol, BS8 1TQ, UK.

Understanding the assembly and dynamics of protein-based supramolecular capsids and cages is of fundamental importance and could lead to applications in synthetic biology and biotechnology. Here we present long and large atomistic molecular dynamics simulations of de novo designed self-assembling protein nanocages (SAGEs) in aqueous media. Microsecond simulations, comprised of ≈42 million atoms for three pre-formed SAGEs of different charges, in the presence of solutes and solvent have been completed. Read More

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December 2018
1 Read

Importance of Binding Affinity for the Activity of a Metallodendritic Chemical Nuclease.

Pharmaceutics 2018 Dec 3;10(4). Epub 2018 Dec 3.

Department of Medicinal and Applied Chemistry, Kaohsiung Medical University, Shih-Chuan 1st Road 100, Kaohsiung 80708, Taiwan.

A family of (2-pyridyl)amino-modified poly(amidoamine) dendrimer Cu complexes was prepared, and their chemical nuclease activities and binding affinity () levels for DNA plasmid were investigated. The values of the G2 to G6 apodendrimers for DNA plasmid were found to be 7.4, 23, 48, 70, and 280 µM, respectively, using ethidium bromide (EtBr) displacement experiments. Read More

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December 2018

Research on the Application of MWCNTs/PLA Composite Material in the Manufacturing of Conductive Composite Products in 3D Printing.

Micromachines (Basel) 2018 Nov 30;9(12). Epub 2018 Nov 30.

School of Materials Science and Engineering, Southwest Jiaotong University, Chengdu 610031, China.

As an advanced manufacturing technology that has been developed in recent years, three-dimensional (3D) printing of macromolecular materials can create complex-shaped components that cannot be realized by traditional processing. However, only a few types of macromolecular materials are suitable for 3D printing: the structure must have a single function, and manufacturing macromolecular functional devices is difficult. In this study, using poly lactic acid (PLA) as a matrix, conductive composites were prepared by adding various contents of multi-walled carbon nanotubes (MWCNTs). Read More

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November 2018
1.286 Impact Factor

Enhancing CO Adsorption and Separation Properties of Aluminophosphate Zeolites by Isomorphous Heteroatom Substitutions.

ACS Appl Mater Interfaces 2018 Dec 4. Epub 2018 Dec 4.

Department of Physical and Macromolecular Chemistry, Faculty of Science , Charles University in Prague , Prague 2 128 43 , Czech Republic.

Mg, Co-substituted aluminophosphate zeolites with ERI framework topology (denoted as MgAPO-ERI and CoAPO-ERI) have been synthesized under hydrothermal conditions by using N, N, N', N'-tetramethyl-1,6-hexanediamine as organic template. Their CO adsorption properties are investigated in comparison to those of the pure aluminophosphate counterpart AlPO-ERI. CoAPO-ERI shows the highest CO uptake of 57. Read More

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December 2018