2,604 results match your criteria MHR: Basic Science of Reproductive Medicine [Journal]


Autophagy regulates abnormal placentation induced by folate deficiency in mice.

Mol Hum Reprod 2019 Apr 11. Epub 2019 Apr 11.

Laboratory of Reproductive Biology, School of Public Health and Management, Chongqing Medical University, Box 197, No. 1 Yixueyuan Road, Yuzhong District, 400016, Chongqing, PR China and Joint International Research Laboratory of Reproduction & Development, Chongqing Medical University, Box 197, No. 1 Yixueyuan Road, Yuzhong District, 400016, Chongqing, PR China.

Folate deficiency has been linked to a wide range of pregnancydisorders. Most researchabout folate-deficiency has focused on the embryo itself, little attention has been paid to possible effects on theplacenta. According to our results, themorphology of the placenta, endocrine function and the expression of genes involved in placental differentiation were all abnormal in folated-deficient mice on days 10, 12 and 14 of pregnancy. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1093/molehr/gaz022DOI Listing

Cisplatin- and cyclophosphamide-induced primordial follicle depletion is caused by direct damage to oocytes.

Mol Hum Reprod 2019 Apr 6. Epub 2019 Apr 6.

Development and Stem Cells Program, Monash Biomedicine Discovery Institute, and Department of Anatomy and Developmental Biology, Monash University, Clayton, 3800, Australia.

It is well established that DNA-damaging chemotherapies can cause infertility and ovarian endocrine failure by depleting the ovarian reserve of primordial follicles. Currently, no effective pharmacological therapies exist for the preservation of long-term fertility and ovarian function in female cancer patients, due to a limited understanding of the mechanisms of chemotherapy-induced follicle depletion. This study investigated the cellular targets, molecular mechanisms, and temporal course of ovarian reserve depletion following treatment with commonly used chemotherapeutic drugs. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1093/molehr/gaz020DOI Listing

Platelet-derived microparticles from recurrent miscarriage associated with antiphospholipid antibody syndrome influence behaviours of trophoblast and endothelial cells.

Mol Hum Reprod 2019 Apr 3. Epub 2019 Apr 3.

Department of Obstetrics and Gynaecology, Shandong Provincial Hospital Affiliated to Shandong University, 324 Jingwu Street, Jinan, Shandong 250021, China.

Platelet-derived microparticles (PMPs) are a type of microparticle budding from platelets undergoing activation or apoptosis in many autoimmune diseases, including antiphospholipid antibody syndrome (APS). PMPs may also contribute to recurrent recurrent miscarriage, although the exact mechanism is unclear. The aim of this study was to determine the potential biological mechanism by which abnormal PMP activation may affect recurrent miscarriage. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1093/molehr/gaz019DOI Listing
April 2019
2 Reads
3.747 Impact Factor

Emerging concepts of shear stress in placental development and function.

Mol Hum Reprod 2019 Apr 1. Epub 2019 Apr 1.

Academic department of Obstetrics and Gynaecology, Level 9, Worsley building, University of Leeds, LS2 9JT.

Blood flow, and the force it generates, is critical to placental development and function throughout pregnancy. This mechanical stimulation of cells by the friction generated from flow is called shear stress (SS) and is a fundamental determinant of vascular homeostasis, regulating remodelling and vasomotor tone. This review describes how SS is fundamental to the establishment and regulation of the blood flow through the uteroplacental and fetoplacental circulations. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1093/molehr/gaz018DOI Listing

Pro-inflammatory cytokine-induced microRNA-212-3p expression promotes myocyte contraction via methyl-CpG-binding protein 2: a novel mechanism for infection-related preterm parturition.

Mol Hum Reprod 2019 Mar 20. Epub 2019 Mar 20.

Department of Obstetrics, Shanghai Obstetrics and Gynecology Hospital of Fudan University, Huang Pu District, Shanghai 200011, People's Republic of China.

Preterm labour is a common pregnancy complication contributing to major maternal and fetal morbidity and mortality. We have found microRNA (miR)-212-3p, a potential infection-associated molecule, was significantly over-expressed during human preterm labour. However, the mechanism remains unknown. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1093/molehr/gaz005DOI Listing

The DNA methylation profile of human spermatogonia at single-cell- and single-allele-resolution refutes its role in spermatogonial stem cell function and germ cell differentiation.

Mol Hum Reprod 2019 Mar 20. Epub 2019 Mar 20.

Institute of Human Genetics, University Medical Center of the Johannes Gutenberg University Mainz, Mainz, Germany.

Human spermatogonial stem cells (hSSCs) have potential in fertility preservation of pre-pubertal boys or in treatment of male adults suffering from meiotic arrest. Prior to therapeutic application, in-vitro propagation of rare hSSCs is mandatory. As the published data points to epigenetic alterations in long-term cell culture of spermatogonia (SPG), an initial characterisation of their DNA methylation state is important. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1093/molehr/gaz017DOI Listing

ATM expression is attenuated by promoter hypermethylation in human ovarian endometriotic stromal cells.

Mol Hum Reprod 2019 Mar 14. Epub 2019 Mar 14.

Department of Obstetrics and Gynecology, Faculty of Medicine, Oita University, Oita 879-5593, Japan.

A number of genes involved in the pathogenesis of endometriosis are silenced by the hypermethylation of their promoter regions. We assessed the effect and mechanism of the DNA demethylating agent 5-aza-2'-deoxycytidine (5-aza-dC) (10 μM) on the cell cycle in human endometriotic cyst stromal cells (ECSCs) and normal endometrial stromal cells (NESCs) by flow cytometry. The DNA methylation status of G2/M checkpoint regulators were investigated by methylation-specific polymerase chain reaction (PCR). Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1093/molehr/gaz016DOI Listing
March 2019
2 Reads

MicroRNA mimics that target the placental renin-angiotensin system inhibit trophoblast proliferation.

Mol Hum Reprod 2019 Apr;25(4):218-227

Priority Research Centre for Reproductive Sciences, School of Biomedical Sciences and Pharmacy, Faculty of Health and Medicine, University of Newcastle, Callaghan, New South Wales, Australia.

In early gestation, the human placental renin-angiotensin system (RAS) is upregulated and plays a role in placental development. Among other functions, signalling through the angiotensin II type 1 receptor (AT1R) initiates proliferation. Many microRNAs (miRNAs) targeting placental RAS mRNAs are downregulated at this time. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1093/molehr/gaz010DOI Listing

Differential cell death decisions in the testis: evidence for an exclusive window of ferroptosis in round spermatids.

Mol Hum Reprod 2019 Mar 13. Epub 2019 Mar 13.

Priority Research Centre for Reproductive Science, School of Environmental and Life Sciences, Discipline of Biological Sciences, University of Newcastle, Callaghan, New South Wales, Australia.

Oxidative stress is a major aetiology in many pathologies, including that of male infertility. Recent evidence in somatic cells has linked oxidative stress to the induction of a novel cell death modality termed ferroptosis. However, the induction of this iron-regulated, caspase-independent cell death pathway has never been explored outside of the soma. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1093/molehr/gaz015DOI Listing
March 2019
3.747 Impact Factor

Investigating the role of CD44 and hyaluronate in embryo-epithelial interaction using an in vitro model.

Mol Hum Reprod 2019 Mar 13. Epub 2019 Mar 13.

Maternal and Fetal Health Centre and Division of Developmental Biology and Medicine, Faculty of Biology, Medicine and Health, University of Manchester, Manchester Academic Health Sciences Centre, St Mary's Hospital, Manchester, UK.

Implantation failure is an important impediment to increasing success rates in assisted reproductive technologies. Knowledge of the cascade of morphological and molecular events at implantation remains limited. Cell surface CD44 and hyaluronate (HA) have been reported in the uterus, but a role in intercellular interaction at implantation remains to be evaluated. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1093/molehr/gaz011DOI Listing

Dysregulated miR-142, -33b, and -423 in granulosa cells target TGFBR1 and SMAD7: a possible role in polycystic ovary syndrome.

Mol Hum Reprod 2019 Mar 13. Epub 2019 Mar 13.

Department of Reproductive Medicine, The Second Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan, China.

It is well established that microRNA (miRNA) expression profiles are altered in patients with polycystic ovary syndrome (PCOS). In addition, abnormal transforming growth factor beta (TGFB) signaling in granulosa cells is related to the pathological conditions of PCOS. However, the function of dysregulated miRNAs in PCOS is still unclear. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1093/molehr/gaz014DOI Listing
March 2019
1 Read
3.747 Impact Factor

Adipose tissue-derived stem cells boost vascularization in grafted ovarian tissue by growth factor secretion and differentiation into endothelial cell lineages.

Mol Hum Reprod 2019 Apr;25(4):184-193

Pôle de Recherche en Gynécologie, Institut de Recherche Expérimentale et Clinique, Université Catholique de Louvain, Av. E. Mounier 52, Brussels, Belgium.

Adipose tissue-derived stem cells (ASCs) have multilineage differentiation potential, proangiogenic properties, and the ability to enhance vascularization in xenografted human ovarian tissue. The aim of the present study was to identify the mechanisms behind the proangiogenic effects of ASCs. For this purpose, severe combined immunodeficient (SCID) mice were grafted with frozen-thawed human ovarian tissue. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1093/molehr/gaz008DOI Listing
April 2019
2 Reads

Modulatory mechanisms of sliding of nine outer doublet microtubules for generating planar and half-helical flagellar waves.

Authors:
Sumio Ishijima

Mol Hum Reprod 2019 Mar 2. Epub 2019 Mar 2.

School of Life Science and Technology, Tokyo Institute of Technology, O-okayama, Tokyo, Japan.

It has been shown that sperm flagellar motility is generated and modulated by metachronal sliding and two types of synchronous sliding of the outer doublet microtubules. Metachronal sliding propagates around the axoneme circumferentially from one doublet to another along the flagellum, whereas the two types of synchronous sliding occur synchronously throughout an extended region along the doublet microtubules. Oscillatory synchronous sliding occurs between most pairs of the nine doublet microtubules, whereas non-oscillatory synchronous sliding occurs between a specific pair of the nine doublet microtubules. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1093/molehr/gaz012DOI Listing
March 2019
2 Reads

A single post-ovulatory dose of ulipristal acetate impairs post-fertilization events in mice.

Mol Hum Reprod 2019 Mar 2. Epub 2019 Mar 2.

Instituto de Biología y Medicina Experimental (IBYME-CONICET), Buenos Aires, Argentina.

Ulipristal acetate (UPA) is a selective progesterone receptor modulator used for emergency contraception that has proven to be highly effective in preventing pregnancy when taken up to 120 h after unprotected sexual intercourse. Even though it may act mainly by delaying or inhibiting ovulation, additional effects of UPA on post-fertilization events cannot be excluded. Therefore, the aim of this study was to determine whether a single post-ovulatory dose of UPA could prevent pregnancy using the mouse as a pre-clinical model. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1093/molehr/gaz013DOI Listing
March 2019
3 Reads

Isoform-specific GSK3A activity is negatively correlated with human sperm motility.

Mol Hum Reprod 2019 Apr;25(4):171-183

Kent State University, Kent, OH, USA.

In mouse and bovine sperm, GSK3 activity is inversely proportional to motility. Targeted disruption of the GSK3A gene in testis results in normal spermatogenesis, but mature sperm present a reduced motility, rendering male mice infertile. On the other hand, GSK3B testis-specific KO is fertile. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1093/molehr/gaz009DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6440577PMC
April 2019
2 Reads

Generation of immortalized human endometrial stromal cell lines with different endometriosis risk genotypes.

Mol Hum Reprod 2019 Apr;25(4):194-205

Department of Obstetrics and Gynaecology and Gynaecology Research Centre, Royal Women's Hospital, University of Melbourne, Cnr Grattan Street and Flemington Road, Parkville, Victoria, Australia.

Endometriotic lesions are composed in part of endometrial-like stromal cells, however, there is a shortage of immortalized human endometrial stromal cultures available for research. As genetic factors play a role in endometriosis risk, it is important that genotype is also incorporated into analysis of pathological mechanisms. Human telomerase reverse transcriptase (hTERT) immortalization (using Lenti-hTERT-green fluorescent protein virus) took place following genotype selection; 13 patients homozygous for either the risk or non-risk 'other' allele for one or more important endometriosis risk single nucleotide polymorphism on chromosome 1p36. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1093/molehr/gaz006DOI Listing
April 2019
3 Reads

Genes upregulated in the amnion at labour are bivalently marked by activating and repressive histone modifications.

Mol Hum Reprod 2019 Apr;25(4):228-240

Mothers and Babies Research Centre, Hunter Medical Research Institute, New Lambton Heights, New South Wales, Australia.

Inflammatory genes are expressed increasingly in the foetal membranes at late gestation triggering birth. Here we have examined whether epigenetic histone modifications contribute to the upregulation of proinflammatory genes in the amnion in late pregnancy and at labour. Amnion samples were collected from early pregnancy, at term in the absence of labour and after spontaneous birth. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1093/molehr/gaz007DOI Listing
April 2019
5 Reads

The role of oxygen in regulating microRNAs in control of the placental renin-angiotensin system.

Mol Hum Reprod 2019 Apr;25(4):206-217

Priority Research Centre for Reproductive Sciences, School of Biomedical Sciences and Pharmacy, Faculty of Health and Medicine, University of Newcastle, University Drive, Callaghan, New South Wales, Australia.

Human placental renin-angiotensin system (RAS) expression is highest in early gestation, at a time when placental oxygen tension is at its lowest (1-3%), and promotes placental development. Some miRNAs predicted to target RAS mRNAs are downregulated in early gestation. We tested the hypothesis that low oxygen suppresses expression of miRNAs that target placental RAS mRNAs, thus increasing concentrations of RAS mRNAs. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1093/molehr/gaz004DOI Listing
April 2019
2 Reads

Combined proteomic and miRNome analyses of mouse testis exposed to an endocrine disruptors chemicals mixture reveals altered toxicological pathways involved in male infertility.

Mol Hum Reprod 2019 Mar;25(3):156-169

Department of Cellular and Molecular Biology, Centro de Investigaciones Biologicas (CIB-CSIC), Madrid, Spain.

The increase in male idiopathic infertility has been associated with daily exposure to endocrine disruptors chemicals (EDCs). Nevertheless, the mechanisms of action in relation to dysregulating proteins and regulatory microRNAs are unknown. We combined proteomic and miRNome analyses of mouse testis chronically exposed to low doses of a define mixture of EDCs [phthalates: bis(2-ethylhexyl), dibutyl and benzyl-butyl; 4-nonylphenol and 4-tert-octylphenol], administered in the drinking water from conception until adulthood (post-natal Day 60/75) and compared them with no-exposed control mice. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1093/molehr/gaz003DOI Listing
March 2019
3 Reads
3.747 Impact Factor

Temporal patterns of gene regulation and upstream regulators contributing to major developmental transitions during Rhesus macaque preimplantation development.

Mol Hum Reprod 2019 Mar;25(3):111-123

Department of Animal Science and Reproductive and Developmental Sciences Program, Michigan State University, East Lansing, MI, USA.

The preimplantation period of life in mammals encompasses a tremendous amount of restructuring and remodeling of the embryonic genome and reprogramming of gene expression. These vast changes support metabolic activation and cellular processes that drive early cleavage divisions and enable the creation of the earliest primitive cell lineages. A major question in mammalian embryology is how such vast, sweeping changes in gene expression are orchestrated, so that changes in gene expression are exactly appropriate to meet the developmental needs of the embryo over time. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1093/molehr/gaz001DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6417155PMC
March 2019
3 Reads

Involvement of endoplasmic reticulum stress in regulation of endometrial stromal cell invasiveness: possible role in pathogenesis of endometriosis.

Mol Hum Reprod 2019 Mar;25(3):101-110

Department of Obstetrics and Gynecology, Samsung Medical Center, Sungkyunkwan University School of Medicine, 50 Irwon-dong, Gangnam-gu, Seoul, Korea.

Endoplasmic reticulum (ER) stress is known to reduce invasiveness in some cancer cells by inhibiting the AKT/mTOR pathway. A previous study from our laboratory suggested that ER stress is promoted by progesterone in human endometrial cells, which suggests that progesterone may inhibit endometrial cell invasiveness by up-regulating ER stress. Therefore, aberrant ER stress in response to progesterone may contribute to the altered invasiveness found in endometriotic tissues. Read More

View Article

Download full-text PDF

Source
https://academic.oup.com/molehr/advance-article/doi/10.1093/
Publisher Site
http://dx.doi.org/10.1093/molehr/gaz002DOI Listing
March 2019
10 Reads

Membrane-bound Glucose regulated protein 78 interacts with alpha-2-macroglobulin to promote actin reorganization in sperm during epididymal maturation.

Mol Hum Reprod 2019 Mar;25(3):137-155

Department of Gamete Immunobiology, ICMR-National Institute for Research in Reproductive Health, Mumbai, India.

Glucose regulated protein 78 (GRP78) is expressed on cell surface in exceptional conditions as seen in cancer cells and macrophages. We have reported its membrane localization in sperm. The functional significance of its surface localization in sperm is an enigma. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1093/molehr/gay055DOI Listing
March 2019
4 Reads

Gonadotrophin-mediated miRNA expression in testis at onset of puberty in rhesus monkey: predictions on regulation of thyroid hormone activity and DLK1-DIO3 locus.

Mol Hum Reprod 2019 Mar;25(3):124-136

Department of Obstetrics, Gynecology and Reproductive Sciences, University of Pittsburgh School of Medicine and Magee-Womens Research Institute, 204 Craft Avenue, Pittsburgh, PA, USA.

Molecular mechanisms responsible for the initiation of primate spermatogenesis remain poorly characterized. Previously, 48 h stimulation of the testes of three juvenile rhesus monkeys with pulsatile LH and FSH resulted in down-regulation of a cohort of genes recognized to favor spermatogonia stem cell renewal. This change in genetic landscape occurred in concert with amplification of Sertoli cell proliferation and the commitment of undifferentiated spermatogonia to differentiate. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1093/molehr/gay054DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6396851PMC
March 2019
4 Reads

ZEB2, a master regulator of the epithelial-mesenchymal transition, mediates trophoblast differentiation.

Mol Hum Reprod 2019 Feb;25(2):61-75

Department of Obstetrics and Gynecology, Division of Maternal Fetal Medicine and Surgery and Center for Abnormal Placentation, Hackensack University Medical Center, Hackensack, NJ, USA.

Study Question: Does the upregulation of the zinc finger E-box binding homeobox 2 (ZEB2) transcription factor in human trophoblast cells lead to alterations in gene expression consistent with an epithelial-mesenchymal transition (EMT) and a consequent increase in invasiveness?

Summary Answer: Overexpression of ZEB2 results in an epithelial-mesenchymal shift in gene expression accompanied by a substantial increase in the invasive capacity of human trophoblast cells.

What Is Known Already: In-vivo results have shown that cytotrophoblast differentiation into extravillous trophoblast involves an epithelial-mesenchymal transition. The only EMT master regulatory factor which shows changes consistent with extravillous trophoblast EMT status and invasive capacity is the ZEB2 transcription factor. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1093/molehr/gay053DOI Listing
February 2019
21 Reads

Exosome-mediated intracellular signalling impacts the development of endometriosis-new avenues for endometriosis research.

Mol Hum Reprod 2019 Jan;25(1):2-4

Robinson Research Institute and Adelaide Medical School, The University of Adelaide, Adelaide, South Australia, Australia.

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1093/molehr/gay050DOI Listing
January 2019
3 Reads

Phenotypic variations in transferred progeny due to genotype of surrogate mother.

Mol Hum Reprod 2019 Feb;25(2):88-99

Department of Experimental Animal Genetic Resources, Laboratory of the Genetics of Experimental Animals, Institute of Cytology and Genetics SB RAS, Novosibirsk, Russia.

Study Question: Does the genotype of the surrogate mother modulate the body composition and immunity of her offspring?

Summary Answer: C57BL/6J (B6) progenies carried by immunodeficient NOD SCID (NS) mothers had increased adaptive but decreased innate, immune responsiveness in comparison with the same genotype offspring carried by immunocompetent mothers, B6 and BALB/c (C); the B6 progenies carried by the same genotype mothers also showed higher body fat than the others.

What Is Known Already: Differences in the major histocompatibility complex (MHC) genes between mother and foetus is considered as an important factor in prenatal embryo development, whereas the impact of such dissimilarity on the phenotype of the mature progeny is unclear.

Study Design, Size, Duration: Transplantation of two-cell mouse embryos into recipient females of the different MHC (H2) genotypes was used as an approach to simulate three variants of the immunogenic mother-foetus interaction: (i) bidirectional immunogenic dialogue between B6 (H2b haplotype) embryos and C (H2d haplotype) surrogate mother; (ii) one-way immunogenic interaction between B6 embryos and immunodeficient NS (H2g7 haplotype) surrogate mother and (iii) reduced immunogenetic dialogue between embryos and surrogate mother of the same H2b haplotype resulting in only a maternal response to HY antigens of male foetuses. Read More

View Article

Download full-text PDF

Source
http://fdslive.oup.com/www.oup.com/pdf/production_in_progres
Publisher Site
http://dx.doi.org/10.1093/molehr/gay052DOI Listing
February 2019
26 Reads

MHR | driving reproductive science.

Authors:

Mol Hum Reprod 2019 Jan;25(1)

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1093/molehr/gay051DOI Listing
January 2019
2 Reads

Macrophages alternatively activated by endometriosis-exosomes contribute to the development of lesions in mice.

Mol Hum Reprod 2019 Jan;25(1):5-16

Department of Obstetrics and Gynecology, Qilu Hospital of Shandong University, Jinan, China.

Study Question: Do exosomes play a role in the pathogenesis of endometriosis in a murine model?

Summary Answer: Exosomes from endometriosis (EMS) can alternatively activate macrophages and thus contribute to the development of lesions in mice.

What Is Known Already: The pathogenesis of endometriosis, an inflammatory disease, possibly involves peritoneal macrophages. Exosomes are recognized as a new communicator among cells and a key modulator in several inflammatory diseases. Read More

View Article

Download full-text PDF

Source
https://academic.oup.com/molehr/advance-article/doi/10.1093/
Publisher Site
http://dx.doi.org/10.1093/molehr/gay049DOI Listing
January 2019
17 Reads

Studying the nuts and bolts of spermatozoa.

Authors:
Allan Pacey

Mol Hum Reprod 2018 Dec;24(12):565-566

Department of Oncology and Metabolism, University of Sheffield, Level 4, The Jessop Wing, Tree Root Walk, Sheffield, UK.

View Article

Download full-text PDF

Source
https://academic.oup.com/molehr/advance-article/doi/10.1093/
Publisher Site
http://dx.doi.org/10.1093/molehr/gay047DOI Listing
December 2018
13 Reads

RHOA activity in expanding blastocysts is essential to regulate HIPPO-YAP signaling and to maintain the trophectoderm-specific gene expression program in a ROCK/actin filament-independent manner.

Mol Hum Reprod 2019 Feb;25(2):43-60

Institute for Biogenesis Research, Department of Anatomy, Biochemistry and Physiology, John A. Burns School of Medicine, University of Hawaii at Manoa, Honolulu, HI, USA.

Study Question: What molecular signals are required to maintain the functional trophectoderm (TE) during blastocyst expansion of the late stage of preimplantation development?

Summary Answer: The activity of ras homology family member A (RHOA) GTPases is necessary to retain the expanded blastocyst cavity and also to sustain the gene expression program specific to TE.

What Is Known Already: At the early stages of preimplantation development, the precursor of the TE lineage is generated through the molecular signals that integrate RHOA, RHO-associated coiled-coil containing protein kinase (ROCK), the apicobasal cell polarity, and the HIPPO-Yes-associated protein (YAP) signaling pathway. By contrast, molecular mechanisms regulating the maintenance of the TE characteristics at the later stage, which is crucial for blastocyst hatching and implantation, are scarcely understood. Read More

View Article

Download full-text PDF

Source
https://academic.oup.com/molehr/advance-article/doi/10.1093/
Publisher Site
http://dx.doi.org/10.1093/molehr/gay048DOI Listing
February 2019
7 Reads

Enhanced expression of TACE contributes to elevated levels of sVCAM-1 in endometriosis.

Mol Hum Reprod 2019 Feb;25(2):76-87

Department of Obstetrics and Gynecology, Medical University of Vienna, Waehringer Guertel 18-20, Vienna, Austria.

Study Question: Are increased sVCAM-1 and sICAM-1 levels associated with tumor necrosis factor-alpha-converting enzyme (TACE) activity in endometriosis?

Summary Answer: Here we provide the first functional evidence that induced TACE activity in human endometriotic epithelial cells is at least in part responsible for the enhanced release of sVCAM-1 from these cells.

What Is Known Already: We and others have shown that serum-soluble (s)VCAM-1 levels are significantly higher in women with endometriosis, compared to disease-free controls. Experimental evidence exists suggesting a role of sICAM-1 and sVCAM-1 in the pathogenesis of endometriosis. Read More

View Article

Download full-text PDF

Source
https://academic.oup.com/molehr/advance-article/doi/10.1093/
Publisher Site
http://dx.doi.org/10.1093/molehr/gay042DOI Listing
February 2019
15 Reads

Probing human sperm metabolism using 13C-magnetic resonance spectroscopy.

Mol Hum Reprod 2019 Jan;25(1):30-41

Academic Unit of Reproductive & Developmental Medicine, Department of Oncology and Metabolism, University of Sheffield, Level 4, The Jessop Wing, Tree Root Walk, Sheffield, UK.

Study Question: Can 13C-Magnetic Resonance Spectroscopy (MRS) of selected metabolites provide useful information about human sperm metabolism and how glycolysis or oxidative phosphorylation are used by different sperm populations?

Summary Answer: Sperm populations, prepared by density gradient centrifugation (DGC) and incubated with either 13Cu-glucose, 13Cu-fructose or 13C1-pyruvate, showed consistent evidence of metabolism generating principally lactate and more intermittently bicarbonate, and significantly more lactate was produced from 13Cu-glucose by vital or motile sperm recovered from the 40/80% interface compared to those from the pellet, which could not be accounted for by differences in the non-sperm cells present.

What Is Known Already: Previous studies have focused on CO2 or other specific metabolite production by human sperm and there remains considerable debate about whether glycolysis and/or oxidative phosphorylation is the more important pathway for ATP production in sperm.

Study Design, Size, Duration: Sperm populations were prepared by DGC and subjected to 13C-MRS to answer the following questions. Read More

View Article

Download full-text PDF

Source
https://academic.oup.com/molehr/advance-article/doi/10.1093/
Publisher Site
http://dx.doi.org/10.1093/molehr/gay046DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6314230PMC
January 2019
27 Reads

MicroRNAs involved in the HMGA2 deregulation and its co-occurrence with MED12 mutation in uterine leiomyoma.

Mol Hum Reprod 2018 Nov;24(11):556-563

Department of Clinical Genetics, Vejle Hospital, Institute of Regional Health Research, University of Southern Denmark, DK, Denmark.

Study Question: Can the mediator complex subunit 12 (MED12) mutation and high mobility group AT-hook 2 (HMGA2) overexpression co-occurrence be explained by the alternative mechanism of HMGA2 dysregulation in uterine leiomyomas (UL)?

Summary Answer: The co-occurrence of MED12 mutation and HMGA2 overexpression, and a negative correlation of five validated or predicted microRNAs that target HMGA2 were reported.

What Is Known Already: The recent stratification of UL, according to recurrent and mutually exclusive genomic alterations affecting HMGA2, MED12, fumarate hydratase (FH) and collagen type IV alpha 5-alpha 6 (COL4A5-COL4A6) pointed out the involvement of distinct molecular pathways. However, the mechanisms of regulation involving these drivers are poorly explored. Read More

View Article

Download full-text PDF

Source
https://academic.oup.com/molehr/article/24/11/556/5078567
Publisher Site
http://dx.doi.org/10.1093/molehr/gay037DOI Listing
November 2018
7 Reads

Down-regulation of long non-coding RNA MALAT1 inhibits granulosa cell proliferation in endometriosis by up-regulating P21 via activation of the ERK/MAPK pathway.

Mol Hum Reprod 2019 Jan;25(1):17-29

Center for Reproductive Medicine, Department of Gynecology and Obstetrics, Nanfang Hospital, Southern Medical University, Guangzhou, People's Republic of China.

Study Question: Is there a specific mechanism underlying the association between lung adenocarcinoma transcript 1 (MALAT1) and endometriosis-related infertility?

Summary Answer: The down-regulation of MALAT1 in endometriosis granulosa cells (GCs) may have an adverse effect on the growth and development of oocytes by inhibiting GC proliferation, due to cell cycle-dependent mechanisms that enhance P21 expression through activation of the extracellular signal-regulated kinase (ERK)/mitogen-activated protein kinase (MAPK) pathway.

What Is Known Already: The association between endometriosis and infertility is well supported throughout the literature, and endometriosis per se and its surgical treatment have an adverse effect on the ovarian reserve and on oocyte development. MALAT1, one of the most extensively expressed and evolutionarily conserved transcripts, has been implicated to play a role in human development and many diseases. Read More

View Article

Download full-text PDF

Source
https://academic.oup.com/molehr/advance-article/doi/10.1093/
Publisher Site
http://dx.doi.org/10.1093/molehr/gay045DOI Listing
January 2019
9 Reads

Activation of latent transforming growth factor-β1, a conserved function for pregnancy-specific beta 1-glycoproteins.

Mol Hum Reprod 2018 Dec;24(12):602-612

Department of Pathology, Uniformed Services University of the Health Sciences, Bethesda, MD, USA.

Study Question: Do all 10 human pregnancy-specific beta 1-glycoproteins (PSGs) and murine PSG23 activate latent transforming growth factor-β1 (TGF-β1)?

Summary Answer: All human PSGs and murine PSG23 activated latent TGF-β1.

What Is Known Already: Two of the 10 members of the PSG1 family, PSG1 and PSG9, were previously shown to activate the soluble small latent complex of TGF-β1, a cytokine with potent immune suppressive functions.

Study Design, Size, Duration: Recombinant PSGs were generated and tested for their ability to activate the small latent complex of TGF-β1 in a cell-free ELISA-based assay and in a bioassay. Read More

View Article

Download full-text PDF

Source
https://academic.oup.com/molehr/advance-article/doi/10.1093/
Publisher Site
http://dx.doi.org/10.1093/molehr/gay044DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6262632PMC
December 2018
14 Reads

Concordance between different trophectoderm biopsy sites and the inner cell mass of chromosomal composition measured with a next-generation sequencing platform.

Mol Hum Reprod 2018 Dec;24(12):593-601

Department of Obstetrics and Gynecology, National Taiwan University Hospital and College of Medicine, No. 8, Chung-Shan South Road, Taipei City, Taiwan (ROC).

Study Question: In PGS, does chromosomal constitution differ among trophectoderm (TE) biopsy sites and between them and the inner cell mass (ICM)?

Summary Answer: The ploidy concordance between ICM and TE was independent of whether the biopsy site in the TE was near to or far from the ICM.

What Is Known Already: TE biopsies are considered less harmful to developing embryos than blastomere biopsies. Removal of multi-cellular samples permits high-resolution next-generation sequencing (Veriseq NGS) to detect aneuploidy present in a minority of cells (mosaicism of diploid and aneuploid cells). Read More

View Article

Download full-text PDF

Source
https://academic.oup.com/molehr/advance-article/doi/10.1093/
Publisher Site
http://dx.doi.org/10.1093/molehr/gay043DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6262631PMC
December 2018
14 Reads

Mammalian sperm contain two factors for calcium release and egg activation: Phospholipase C zeta and a cryptic activating factor.

Authors:
Keith T Jones

Mol Hum Reprod 2018 10;24(10):465-468

Faculty of Sciences, University of Adelaide, 5005, South Australia, Australia, and School of Biological Sciences, Faculty of Environmental and Life Sciences, University of Southampton, UK.

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1093/molehr/gay038DOI Listing
October 2018
2 Reads

Age-related gene expression profiles of immature human oocytes.

Mol Hum Reprod 2018 10;24(10):469-477

Center for Reproductive Medicine, Academic Medical Center, University of Amsterdam, Meibergdreef 9, Amsterdam, The Netherlands.

Study Question: What is the difference between the gene expression profiles of single human germinal vesicle (GV) oocytes from women of different ages?

Summary Answer: There were no statistically significant differences in gene expression profiles of human GV oocytes from women of different ages (range: 25-43).

What Is Known Already: It is well established that reproductive capacity declines as women age, which is attributed to oocyte quality since this decline is counterbalanced in older women receiving young donor oocytes. Altered gene expression of human oocytes at different stages of development in relation to female age is one of the suggested mechanisms that could explain the decrease in oocyte quality. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1093/molehr/gay036DOI Listing
October 2018
3 Reads
3.750 Impact Factor

Janus kinase JAK1 maintains the ovarian reserve of primordial follicles in the mouse ovary.

Mol Hum Reprod 2018 Nov;24(11):533-542

School of Environmental and Life Sciences, University of Newcastle, Callaghan, NSW, Australia.

Study Question: Is the Janus kinase and signal transducer and activator of transcription (JAK-STAT) signalling pathway involved in ovarian follicle development and primordial follicle activation?

Summary Answer: JAK1 is a key factor involved in the regulation of primordial follicle activation and maintenance of the ovarian reserve.

What Is Known Already: A series of integrated, intrinsic signalling pathways (including PI3K/AKT, mTOR and KITL) are responsible for regulating the ovarian reserve of non-growing primordial follicles and ultimately female fertility. The JAK-STAT signal transduction pathway is highly conserved with established roles in cell division and differentiation. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1093/molehr/gay041DOI Listing
November 2018
4 Reads
3.750 Impact Factor

Transcriptional landscape changes during human embryonic stem cell derivation.

Mol Hum Reprod 2018 Nov;24(11):543-555

Ghent-Fertility and Stem cell Team (G-FaST), Department for Reproductive Medicine, Ghent University Hospital, Ghent, Belgium.

Study Question: What are the transcriptional changes occurring during the human embryonic stem cell (hESC) derivation process, from the inner cell mass (ICM) to post-ICM intermediate stage (PICMI) to hESC stage, that have downstream effects on pluripotency states and differentiation?

Summary Answer: We reveal that although the PICMI is transcriptionally similar to the hESC profile and distinct from ICM, it exhibits upregulation of primordial germ cell (PGC) markers, dependence on leukemia inhibitory factor (LIF) signaling, upregulation of naïve pluripotency-specific signaling networks and appears to be an intermediate switching point from naïve to primed pluripotency.

What Is Known Already: It is currently known that the PICMI exhibits markers of early and late-epiblast stage. It is suggested that hESCs acquire primed pluripotency features due to the upregulation of post-implantation genes in the PICMI which renders them predisposed towards differentiation cues. Read More

View Article

Download full-text PDF

Source
https://academic.oup.com/molehr/advance-article/doi/10.1093/
Publisher Site
http://dx.doi.org/10.1093/molehr/gay039DOI Listing
November 2018
33 Reads

The functional anatomy of the human spermatozoon: relating ultrastructure and function.

Authors:
David Mortimer

Mol Hum Reprod 2018 Dec;24(12):567-592

Oozoa Biomedical Inc., Caulfeild Village, West Vancouver, BC, Canada.

The Internet, magazine articles, and even biomedical journal articles, are full of cartoons of spermatozoa that bear minimal resemblance to real spermatozoa, especially human spermatozoa, and this had led to many misconceptions about what spermatozoa look like and how they are constituted. This review summarizes the historical and current state of knowledge of mammalian sperm ultrastructure, with particular emphasis on and relevance to human spermatozoa, combining information obtained from a variety of electron microscopic (EM) techniques. Available information on the composition and configuration of the various ultrastructural components of the spermatozoon has been related to their mechanistic purpose and roles in the primary aspects of sperm function and fertilization: motility, hyperactivation, capacitation, the acrosome reaction and sperm-oocyte fusion. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1093/molehr/gay040DOI Listing
December 2018
3 Reads

Does the molecular and metabolic profile of human granulosa cells correlate with oocyte fate? New insights by Fourier transform infrared microspectroscopy analysis.

Mol Hum Reprod 2018 Nov;24(11):521-532

9.baby, Family and Fertility Center, Via Dante, 15, Bologna, Italy.

Study Question: Does the molecular and metabolic profile of human mural granulosa cells (GCs) correlate with oocyte fate?

Summary Answer: A close relation between the metabolic profile of mural GCs and the fate of the corresponding oocyte was revealed by the analysis of selected biomarkers defined by GC Fourier transform infrared microspectroscopy (FTIRM) analysis.

What Is Known Already: In ART, oocyte selection is mainly based on the subjective observation of its morphological features; despite recent efforts, the success rate of this practice is still unsatisfactory. FTIRM is a well-established vibrational technique recently applied to evaluate oocytes quality in several experimental models, including human. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1093/molehr/gay035DOI Listing
November 2018
11 Reads

ADAM8 localizes to extravillous trophoblasts within the maternal-fetal interface and potentiates trophoblast cell line migration through a β1 integrin-mediated mechanism.

Mol Hum Reprod 2018 10;24(10):495-509

British Columbia Children's Hospital Research Institute, 950 West 28th Ave, Vancouver, Canada.

Study Question: Does A Disintegrin And Metalloproteinase 8 (ADAM8) control extravillous trophoblast (EVT) differentiation and migration in early human placental development?

Summary Answer: ADAM8 mRNA preferentially localizes to invasive HLA-G-positive trophoblasts, associates with the acquirement of an EVT phenotype and promotes trophoblast migration through a mechanism requiring β1-integrin.

What Is Known Already: Placental establishment in the first trimester of pregnancy requires the differentiation of progenitor trophoblasts into invasive EVTs that produce a diverse repertoire of proteases that facilitate matrix remodeling and activation of signaling pathways important in controlling cell migration. While multiple ADAM proteases, including ADAM8, are highly expressed by invasive trophoblasts, the role of ADAM8 in controlling EVT-related processes is unknown. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1093/molehr/gay034DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6154767PMC
October 2018
3 Reads

Transcriptome analysis of rhesus monkey failed-to-mature oocytes: deficiencies in transcriptional regulation and cytoplasmic maturation of the oocyte mRNA population.

Mol Hum Reprod 2018 10;24(10):478-494

Department of Animal Science and Reproductive and Developmental Sciences Program, Michigan State University, East Lansing, MI, USA.

Study Question: Which different pathways and functions are altered in rhesus monkey oocytes that fail to mature after an ovulatory stimulus?

Summary Answer: Failed to mature (FTM) oocytes complete a large portion of the transition in transcriptome composition associated with normal maturation, but also manifest numerous differences that indicate incomplete transcriptional repression and cytoplasmic maturation affecting multiple processes.

What Is Known Already: Oocyte maturation defects contribute to unexplained female infertility. Failure of some oocytes to undergo germinal vesicle breakdown or progress to second meiotic metaphase in response to an ovulatory stimulus can limit the number of high quality oocytes available for ART. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1093/molehr/gay032DOI Listing
October 2018
7 Reads

Piezo1 channels are mechanosensors in human fetoplacental endothelial cells.

Mol Hum Reprod 2018 10;24(10):510-520

Leeds Institute of Cardiovascular and Metabolic Medicine, LIGHT Laboratories, University of Leeds, 6 Clarendon Way, Leeds, UK.

Study Question: Does the shear stress sensing ion channel subunit Piezo1 have an important mechanotransduction role in human fetoplacental endothelium?

Summary Answer: Piezo1 is present and functionally active in human fetoplacental endothelial cells, and disruption of Piezo1 prevents the normal response to shear stress.

What Is Known Already: Shear stress is an important stimulus for maturation and function of placental vasculature but the molecular mechanisms by which the force is detected and transduced are unclear. Piezo1 channels are Ca2+-permeable non-selective cationic channels which are critical for shear stress sensing and maturation of murine embryonic vasculature. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1093/molehr/gay033DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6311101PMC
October 2018
2 Reads

A Dolce & Gabbana model in every ART clinic?

Mol Hum Reprod 2018 09;24(9):431-432

Reproductive and Developmental Biology, School of Medicine, Ninewells Hospital and Medical School, University of Dundee, Dundee, UK.

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1093/molehr/gay030DOI Listing
September 2018
2 Reads

Regulation of the human placental (pro)renin receptor-prorenin-angiotensin system by microRNAs.

Mol Hum Reprod 2018 09;24(9):453-464

School of Biomedical Sciences and Pharmacy, Faculty of Health and Medicine, University of Newcastle, Callaghan, NSW, Australia.

Study Question: Are any microRNAs (miRNAs) that target the placental renin-angiotensin system (RAS) in the human placenta suppressed in early gestation?

Summary Answer: Overall, 21 miRNAs with predicted RAS mRNA targets were less abundant in early versus term placentae and nine were more highly expressed.

What Is Known Already: Regulation of human placental RAS expression could alter placental development and therefore normal pregnancy outcome. The expression of genes encoding prorenin (REN), angiotensinogen, (pro)renin receptor, angiotensin converting enzyme 2, and the angiotensin II type 1 receptor are highest in early gestation, at a time when oxygen tension is at its lowest. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1093/molehr/gay031DOI Listing
September 2018
6 Reads

A novel transcriptional network for the androgen receptor in human epididymis epithelial cells.

Mol Hum Reprod 2018 09;24(9):433-443

Human Molecular Genetics Program, Lurie Children's Research Center, Chicago, IL, USA.

Study Question: What is the transcriptional network governed by the androgen receptor (AR) in human epididymis epithelial (HEE) cells from the caput region and if the network is tissue-specific, how is this achieved?

Summary Answer: About 200 genes are differentially expressed in the caput HEE cells after AR activation; the AR transcriptional network is tissue-specific and may be mediated in part by distinct AR co-factors including CAAT-enhancer binding protein beta (CEBPB) and runt-related transcription factor 1 (RUNX1).

What Is Known Already: Little is known about the AR transcriptional program genome wide in HEE cells, nor its co-factors in those cells. AR has been best studied in the prostate gland epithelium and prostate cancer cell lines, due to the important role of this factor in prostate cancer. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1093/molehr/gay029DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6454485PMC
September 2018
9 Reads

Education, education, education-now more than ever?

Mol Hum Reprod 2018 08;24(8):426-429

Reproductive and Developmental Biology, School of Medicine, Ninewells Hospital and Medical School, University of Dundee, Dundee, UK.

The generation of scientists and physicians that took part in starting the whole era of modern-assisted reproduction is currently close to retirement or has already left research. A new generation is about to take over and the profession is facing a massive transgenerational transition. Since current treatments have reached a plateau in success rates and costs, new research and development is required to further advance the field. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1093/molehr/gay028DOI Listing
August 2018
18 Reads

IGF2-derived miR-483-3p contributes to macrosomia through regulating trophoblast proliferation by targeting RB1CC1.

Mol Hum Reprod 2018 09;24(9):444-452

State Key Laboratory of Reproductive Medicine, Institute of Toxicology, Nanjing Medical University, Nanjing, China.

Study Question: What is the role of insulin-like growth factor 2 (IGF2)-derived miR-483-3p in macrosomia?

Summary Answer: IGF2-derived intronic miR-483-3p is overexpressed in macrosomia placentas, and miR-483-3p prompts HTR-8/SVneo extravillous trophoblast cell line proliferation through down-regulation of its target RB1 inducible coiled-coil 1 (RB1CC1).

What Is Known Already: Macrosomia is a common pregnancy-associated disease and causes a number of adverse maternal and perinatal outcomes. The development of macrosomia is reportedly attributable to over proliferation of the placental cells. Read More

View Article

Download full-text PDF

Source
http://dx.doi.org/10.1093/molehr/gay027DOI Listing
September 2018
19 Reads