606,945 results match your criteria Lymphocytic Choriomeningitis


TroCCL4, a CC chemokine of Trachinotus ovatus, is involved in the antimicrobial immune response.

Fish Shellfish Immunol 2018 Dec 3. Epub 2018 Dec 3.

State Key Laboratory of Marine Resource Utilization in South China Sea, Hainan University, PR China.

CC chemokines are a large subfamily of chemokines that play an important role in the innate immune system. To date, several CC chemokines have been identified in fish species; however, the activities and functions of these putative chemokines remain ambiguous in teleosts, especially in the golden pompano, Trachinotus ovatus. Here, we characterized CC chemokine ligand 4 from T. Read More

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December 2018
2 Reads

Endonuclease G modulates the alternative splicing of deoxyribonuclease 1 mRNA in human CD4 T lymphocytes and prevents the progression of apoptosis.

Biochimie 2018 Dec 3. Epub 2018 Dec 3.

Institute of Biomedical Chemistry, Pogodinskaya St 10/8, 119121, Moscow, Russia.

Apoptotic endonucleases act cooperatively to fragment DNA and ensure the irreversibility of apoptosis. However, very little is known regarding the potential regulatory links between endonucleases. Deoxyribonuclease 1 (DNase I) inactivation is caused by alternative splicing (AS) of DNase I pre-mRNA skipping exon 4, which occurs in response to EndoG overexpression in cells. Read More

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December 2018
1 Read

Gene landscape and correlation between B-cell infiltration and programmed death ligand 1 expression in lung adenocarcinoma patients from The Cancer Genome Atlas data set.

PLoS One 2018 6;13(12):e0208459. Epub 2018 Dec 6.

Graduate Institute of Medical Sciences, College of Medicine, Taipei Medical University, Taipei, Taiwan.

Tumor-infiltrating lymphocytes are related to positive clinical prognoses in numerous cancer types. Programmed death ligand 1 (PD-L1), a mediator of the PD-1 receptor, plays an inhibitory role in cancer immune responses. PD-L1 upregulation can impede infiltrating T-cell functions in lung adenocarcinoma (LUAD), a lung cancer subtype. Read More

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December 2018
1 Read

Loss of ARHGEF1 causes a human primary antibody deficiency.

J Clin Invest 2018 Dec 6. Epub 2018 Dec 6.

ARHGEF1 is a RhoA-specific guanine nucleotide exchange factor expressed in hematopoietic cells. We used whole-exome sequencing to identify compound heterozygous mutations in ARHGEF1, resulting in the loss of ARHGEF1 protein expression in two primary-antibody-deficient siblings presenting with recurrent severe respiratory tract infections and bronchiectasis. Both ARHGEF1-deficient patients showed an abnormal B cell immunophenotype, with a deficiency in marginal-zone and memory B cells and an increased frequency of transitional B cells. Read More

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December 2018

Platelet to lymphocyte ratio predicts all-cause mortality in patients with carotid arterial disease.

Rom J Intern Med 2018 Dec 1. Epub 2018 Dec 1.

TOBB Economy and Technology University, Cardiology Department, Ankara, Turkey.

Background: Platelet to lymphocyte ratio (PLR) has been demonstrated as an risk and prognostic marker in many of cardiovascular diseases. Relationship between PLR and severity of carotid stenosis has been shown. The aim of our study was to investigate the relationship between PLR and all cause mortality in patients with carotid arterial disease. Read More

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December 2018

The association between markers of tumor cell metabolism, the tumor microenvironment and outcomes in patients with colorectal cancer.

Int J Cancer 2018 Dec 6. Epub 2018 Dec 6.

Unit of Experimental Therapeutics, Institute of Cancer Science, University of Glasgow, Glasgow, UK.

Tumor cell anaerobic metabolism has been reported to be a prognostic factor in colorectal cancer. The present study investigated the association between monocarboxylate transporter (MCT) 1, MCT 2, lactate dehydrogenase (LDH) 1 and LDH 5, the tumor microenvironment, and outcome in patients with colorectal cancer. A cohort of 150 patients with stage I-III CRC were utilised to assess tumor cell expression of MCT-1, MCT-2, LDH-1 and LDH5 by immunohistochemistry. Read More

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December 2018

Impact of HLA allele-KIR pairs on HIV clinical outcome in South Africa.

J Infect Dis 2018 Dec 6. Epub 2018 Dec 6.

Department of Paediatrics, University of Oxford, Oxford, United Kingdom.

Background: HLA class I contributes to HIV immune control through antigen presentation to both cytotoxic T lymphocytes (CTLs) and natural killer (NK) cells. However, by contrast with investigations of CTL, studies of NK cells in HIV control through HLA-killer immunoglobulin-like receptor (KIR) interactions remain sparse in African cohorts.

Methods: 312 treatment naïve, chronically HIV-infected adults were recruited from South Africa, and the effects of HLA-KIR pairs on clinical outcome were statistically analyzed. Read More

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December 2018
1 Read

Overview of non-coding mutations in chronic lymphocytic leukemia.

Mol Oncol 2018 Dec 6. Epub 2018 Dec 6.

Division of Hematology, Oncology Institute of Southern Switzerland, Bellinzona, Switzerland.

Chronic lymphocytic leukemia (CLL) is the most frequent leukemia type in which the genetic alterations influencing the clinico-biological course are not entirely understood. CLL has a heterogeneous course, with some patients showing an indolent course, and others experiencing an aggressive course. Whole-genome sequencing (WGS) and whole-exome sequencing (WES) studies identified recurrently mutated genes in CLL, and profiled its clonal evolution patterns. Read More

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December 2018
1 Read

Rebound effect associated with JAK inhibitor use in the treatment of alopecia areata.

J Eur Acad Dermatol Venereol 2018 Dec 6. Epub 2018 Dec 6.

Department of Dermatology, Tufts Medical Center, Boston, MA.

Alopecia areata (AA) is a common autoimmune disease driven by Th1 cytokines characterized by non-scarring hair loss. Mouse models have demonstrated that IFN-γ-producing NKG2D+ CD8+ cytotoxic T lymphocytes (CTLs) are essential for disease pathogenesis, along with JAK-STAT dependent cytokines IFN-γ and IL-15, which induce autoreactive T cell activation. This article is protected by copyright. Read More

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December 2018
1 Read

High renal DC-SIGN cell density is associated with severe renal lesions and poor prognosis in patients with Immunoglobulin A Nephropathy.

Histopathology 2018 Dec 5. Epub 2018 Dec 5.

Division of Nephrology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology Wuhan, Hubei, China.

Background And Objectives: In this observational cohort study, we assessed the prognostic value of DC-SIGN cells in pathogenesis and progression of IgA nephropathy (IgAN).

Design, Setting, Participants, & Measurements: A total of 139 adult IgAN patients were enrolled in this study from June 2009 to June 2010. We characterized DC-SIGN cells by immunohistochemistry or immunofluorescence in renal biopsy tissue. Read More

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December 2018

CD8 cytotoxic T lymphocytes in cancer immunotherapy: A review.

J Cell Physiol 2018 Nov 22. Epub 2018 Nov 22.

Department of Anatomy, School of Medicine, Kurdistan University of Medical Sciences, Sanandaj, Iran.

CD8 cytotoxic T lymphocytes (CTLs) are preferred immune cells for targeting cancer. During cancer progression, CTLs encounter dysfunction and exhaustion due to immunerelated tolerance and immunosuppression within the tumor microenvironment (TME), with all favor adaptive immune-resistance. Cancer-associated fibroblasts (CAFs), macrophage type 2 (M2) cells, and regulatory T cells (Tregs) could make immunologic barriers against CD8 T cell-mediated antitumor immune responses. Read More

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November 2018
1 Read

Berberis lycium Royle fruit extract mitigates oxi-inflammatory stress by suppressing NF-κB/MAPK signalling cascade in activated macrophages and Treg proliferation in splenic lymphocytes.

Inflammopharmacology 2018 Dec 6. Epub 2018 Dec 6.

Pharmacology and Toxicology Lab, Food and Nutraceutical Division, CSIR-Institute of Himalayan Bioresource Technology, Palampur, HP, 176061, India.

Although Berberis plant species have been advocated as immune modulators, information regarding their mechanism(s) of action is limited. Therefore, in the present study we assessed the efficacy of Berberis lycium Royle fruit extract (BLFE) in the attenuation of lipopolysaccharide (LPS)-induced oxi-inflammatory aggravation and concanavalin A (Con-A)-induced proliferation in murine peritoneal macrophages and lymphocytes, respectively. BLFE strongly suppressed production of the oxidative and inflammatory effector molecules nitric oxide (NO), reactive oxygen species (ROS), inducible nitric oxide synthase (iNOS), inflammatory cytokines (TNF-α/IL-6/IL-1β/IFN-γ) as well as chemokines (MCP-1 and RANTES), with a concomitant enhancement in heme oxygenase-1 (HO-1) and IL-10 levels. Read More

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December 2018
1 Read

Villous lymphocytes in splenic large B-cell lymphoma with diffuse red pulp infiltration.

Int J Hematol 2018 Dec 5. Epub 2018 Dec 5.

Department of Pathology, National Cheng Kung University Hospital, College of Medicine, National Cheng Kung University, Tainan, Taiwan.

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December 2018

PD-1 and PD-L1 Expression in Male Breast Cancer in Comparison with Female Breast Cancer.

Target Oncol 2018 Dec 5. Epub 2018 Dec 5.

Department of Pathology, University Medical Center Utrecht, PO Box 85500, 3508 GA, Utrecht, The Netherlands.

Background: Male breast cancer is rare, as it represents less than 1% of all breast cancer cases. In addition, male breast cancer appears to have a different biology than female breast cancer. Programmed death-1 (PD-1) and its ligand, programmed death-ligand 1 (PD-L1), seem to have prognostic and predictive values in a variety of cancers, including female breast cancer. Read More

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December 2018
1 Read

Prognostic Value of Lymphocyte Activation Gene-3 (LAG-3) Expression in Esophageal Squamous Cell Carcinoma.

J Cancer 2018 20;9(22):4287-4293. Epub 2018 Oct 20.

State Key Laboratory of Oncology in South China, Department of Experimental Research, Sun Yat-sen University Cancer Center, No. 651 Dongfeng East Road, Guangzhou 510060, China.

Esophageal squamous cell carcinoma (ESCC) is a malignant epithelial tumor with a high incidence in East Asia and the Middle East. The outcomes for ESCC patients are usually not optimal due to the recurrence and metastasis. This study is aim to examine the expression and the prognostic value of LAG-3 in ESCC. Read More

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October 2018
1 Read

Predictors of Residual T1 High Grade on Re-Transurethral Resection in a Large Multi-Institutional Cohort of Patients with Primary T1 High-Grade/Grade 3 Bladder Cancer.

J Cancer 2018 20;9(22):4250-4254. Epub 2018 Oct 20.

Division of Urology, European Institute of Oncology, Milan, Italy.

The aim of this multi-institutional study was to identify predictors of residual high-grade (HG) disease at re-transurethral resection (reTUR) in a large cohort of primary T1 HG/Grade 3 (G3) bladder cancer patients. A total of 1155 patients with primary T1 HG/G3 bladder cancer from 13 academic institutions that underwent a reTUR within 6 weeks after first TUR were evaluated. Logistic regression analysis was performed to assess the association of predictive factors with residual HG at reTUR. Read More

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October 2018
1 Read

Sex differences of inflammation in target organs, induced by intraperitoneal injection of lipopolysaccharide, depend on its dose.

J Inflamm Res 2018 8;11:431-445. Epub 2018 Nov 8.

Department of Pathology, Federal State Budgetary Institution "Science Research Institute of Human Morphology", Moscow, Russia.

Purpose: The aim of our research was to study sex differences and the severity of inflammatory changes in target organs and the peculiarities of immunological disorders when low and high doses of lipopolysaccharide (LPS) were administered to rats.

Methods: Male and female 2- to 3-month-old Wistar rats (200-250 g) were injected intraperitoneally with LPS in one of two doses: 1.5 or 15 mg/kg. Read More

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November 2018
1 Read

Sodium selenite inhibits deoxynivalenol-induced injury in GPX1-knockdown porcine splenic lymphocytes in culture.

Sci Rep 2018 Dec 5;8(1):17676. Epub 2018 Dec 5.

College of Veterinary Medicine, Sichuan Agricultural Uniiversity, Sichuan Province Key Laboratory of Animal Disease and Human Health, Key Laboratory of Environmental Hazard and Human Health of Sichuan Province, Chengdu, 611130, China.

Deoxynivalenol (DON) is a cytotoxic mycotoxin that can cause cell damages. The main effect is to inhibit protein synthesis. Oxidative stress is one of the effects of DON. Read More

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December 2018

Cryopreservation of primary B cells minimally influences their signaling responses.

Sci Rep 2018 Dec 5;8(1):17651. Epub 2018 Dec 5.

Centre for Molecular Medicine Norway, Nordic EMBL Partnership, University of Oslo and Oslo University Hospital, Oslo, Norway.

Phospho flow is a powerful approach to detect cell signaling aberrations, identify biomarkers and assess pharmacodynamics, and can be performed using cryopreserved samples. The effects of cryopreservation on signaling responses and the reproducibility of phospho flow measurements are however unknown in many cell systems. Here, B lymphocytes were isolated from healthy donors and patients with the B cell malignancy chronic lymphocytic leukemia and analyzed by phospho flow using phospho-specific antibodies targeting 20 different protein epitopes. Read More

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December 2018

Correction: Synergistic activation of pro-inflammatory type-2 CD8  T lymphocytes by lipid mediators in severe eosinophilic asthma.

Mucosal Immunol 2018 Dec 5. Epub 2018 Dec 5.

Respiratory Medicine Unit and Oxford Biomedical Research Centre, Nuffield Department of Medicine, John Radcliffe Hospital, University of Oxford, Oxford, UK.

This article was originally published under standard licence, but has now been made available under a [CC BY 4.0] license. The PDF and HTML versions of the paper have been modified accordingly. Read More

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December 2018
2 Reads

Tumor-draining lymph nodes are pivotal in PD-1/PD-L1 checkpoint therapy.

JCI Insight 2018 Dec 6;3(23). Epub 2018 Dec 6.

Department of Immunohematology and Blood Transfusion, Leiden University Medical Center (LUMC), Leiden, Netherlands.

PD-1/PD-L1 checkpoint therapy for cancer is commonly considered to act by reactivating T cells in the tumor microenvironment. Here, we present data from 2 mouse tumor models demonstrating an essential involvement of tumor-draining lymph nodes in PD-1 and PD-L1 therapeutic efficacy. Immune activation induced by checkpoint treatment was predominantly observed in the tumor-draining, but not nondraining, lymph nodes and was reflected in local accumulation of CD8+ T cells. Read More

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December 2018
1 Read

Deep learning for detecting tumour-infiltrating lymphocytes in testicular germ cell tumours.

J Clin Pathol 2018 Dec 5. Epub 2018 Dec 5.

Nuffield Department of Surgical Sciences and NIHR Oxford Biomedical Research Centre, University of Oxford, Oxford, UK.

Aims: To evaluate if a deep learning algorithm can be trained to identify tumour-infiltrating lymphocytes (TILs) in tissue samples of testicular germ cell tumours and to assess whether the TIL counts correlate with relapse status of the patient.

Methods: TILs were manually annotated in 259 tumour regions from 28 whole-slide images (WSIs) of H&E-stained tissue samples. A deep learning algorithm was trained on half of the regions and tested on the other half. Read More

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December 2018

Isolation and characterization of camelid single-domain antibodies against HER2.

BMC Res Notes 2018 Dec 5;11(1):866. Epub 2018 Dec 5.

Human Health Therapeutics Research Centre, National Research Council Canada, 100 Sussex Drive, Ottawa, ON, K1A 0R6, Canada.

Objective: To isolate and characterize novel high-affinity llama single-domain antibodies against human HER2.

Results: We immunized a llama with human HER2, constructed a phage-displayed VH library from the lymphocytes of the animal, and isolated six unique HER2-specific VHs by panning. All six VHs were unique at the amino acid level and were clonally unrelated, as reflected by their distinct CDR3 lengths. Read More

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December 2018

Association between autophagy and inflammation in patients with rheumatoid arthritis receiving biologic therapy.

Arthritis Res Ther 2018 Dec 5;20(1):268. Epub 2018 Dec 5.

Rheumatology and Immunology Center, China Medical University Hospital, No. 2, Yude Road, Taichung, 40447, Taiwan.

Background: Increasing evidence indicates a pathogenic role of deregulated autophagy in rheumatoid arthritis (RA). We examined the relationship between autophagy and inflammatory parameters in patients with RA receiving biologic therapy.

Methods: In 72 patients with RA and 20 healthy control subjects (HC), autophagosome levels were determined by the mean fluorescence intensity (MFI) of autophagosomotropic dye incorporated into circulating immune cells, and p62 expression levels in immune cells were measured by flow cytometry. Read More

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December 2018
1 Read

Tumour associated lymphocytes in the pleural effusions of patients with mesothelioma express high levels of inhibitory receptors.

BMC Res Notes 2018 Dec 5;11(1):864. Epub 2018 Dec 5.

National Centre for Asbestos Related Diseases, School of Medicine and Pharmacology, University of Western Australia, Nedlands, Western Australia, Australia.

Objective: Pleural effusion (PE) is a common feature of malignant pleural mesothelioma. These effusions typically contain lymphocytes and malignant cells. We postulated that the PE would be a source of lymphocytes for analysis of tumor immune milieu. Read More

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December 2018

Immunological effects of the intraparenchymal administration of allogeneic and autologous adipose-derived mesenchymal stem cells after the acute phase of middle cerebral artery occlusion in rats.

J Transl Med 2018 Dec 5;16(1):339. Epub 2018 Dec 5.

Department of Pediatrics, Navy General Hospital, No. 6, Fucheng Road, Haidian District, Beijing, 100048, China.

Background: Adipose-derived mesenchymal stem cell (ADMSC) therapy can promote recovery from cerebral ischemia; however, more information regarding appropriate sources of ADMSCs is required. This study was aimed at analyzing the immunogenicity of rat ADMSCs by comparing the immunological effects of intraparenchymal administration of allogeneic ADMSCs (allo-ADMSCs) and autologous ADMSCs (auto-ADMSCs) after the acute phase of middle cerebral artery occlusion (MCAO) in rats.

Methods: Allo- or auto-ADMSCs from rats (1 × 10 cells) were transplanted into Lewis rats 8 days post MCAO. Read More

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December 2018

Effects of Viola tricolor Flower Hydroethanolic Extract on Lung Inflammation in a Mouse Model of Chronic Asthma.

Iran J Allergy Asthma Immunol 2018 Oct 14;17(5):409-417. Epub 2018 Oct 14.

Iranian Center of Neurological Research, Neuroscience Institute, Imam Khomeini Hospital, Tehran University of Medical Sciences, Tehran, Iran AND Department of Physiology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.

Asthma is a chronic inflammatory disease of the lungs driven by T cell activation. Viola tricolor L. as a traditional medical herb could suppress activated T lymphocytes and has been used empirically for asthma remedy. Read More

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October 2018

Fluorescently Tagged CCL19 and CCL21 to Monitor CCR7 and ACKR4 Functions.

Int J Mol Sci 2018 Dec 4;19(12). Epub 2018 Dec 4.

Biotechnology Institute Thurgau (BITg) at the University of Konstanz, CH-8280 Kreuzlingen, Switzerland.

Chemokines are essential guidance cues orchestrating cell migration in health and disease. Cognate chemokine receptors sense chemokine gradients over short distances to coordinate directional cell locomotion. The chemokines CCL19 and CCL21 are essential for recruiting CCR7-expressing dendritic cells bearing pathogen-derived antigens and lymphocytes to lymph nodes, where the two cell types meet to launch an adaptive immune response against the invading pathogen. Read More

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December 2018
1 Read

[Sjögren's Syndrome: Early diagnosis and effective treatment].

Authors:
Torsten Witte

Dtsch Med Wochenschr 2018 Dec 3;143(24):1778-1782. Epub 2018 Dec 3.

Klinik für Immunologie und Rheumatologie der Medizinischen Hochschule Hannover.

Primary Sjögren's syndrome (pSS) is an autoimmune disease affecting the salivary and lachrymal glands. The patients complain of symptoms of dry eyes and dry mouth, but up to 50 % may additionally develop extraglandular manifestations such as arthritis, vasculitis, polyneuropathy, pulmonary fibrosis or interstitial nephritis. Most therapeutic studies on the glandular manifestations of pSS failed to meet their primary endpoints, possibly since many of the patients had already advanced and irreversible disease. Read More

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December 2018
1 Read

HMGB1 blockade significantly improves luminal fibrous obliteration in a murine model of bronchiolitis obliterans syndrome.

Transpl Immunol 2018 Nov 30. Epub 2018 Nov 30.

Department of Surgery and Science, Graduate School of Medical Sciences, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka-shi, Fukuoka 812-8582, Japan.

Background: Although high-mobility group box-1 (HMGB1), which is a nuclear protein, was reported to enhance the allogeneic responses in transplantation, the effect of HMGB1 on bronchiolitis obliterans syndrome (BOS) is unknown.

Methods: A murine heterotopic tracheal transplantation model was used. Protein concentrations of HMGB1, interferon-γ (IFN-γ), interleukin (IL)-10, and IL-17 were analyzed in the isografts, allografts, controls, and HMGB1-neutralizing antibody administered allografts (n = 6; Days 1, 3, 5, 7, 14, 21, and 28). Read More

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November 2018
1 Read

Targeted multigene deep sequencing of Bruton tyrosine kinase inhibitor-resistant chronic lymphocytic leukemia with disease progression and Richter transformation.

Cancer 2018 Dec 3. Epub 2018 Dec 3.

Department of Hematopathology, The University of Texas MD Anderson Cancer Center, Houston, Texas.

Background: In a proportion of patients with chronic lymphocytic leukemia (CLL), resistance to Bruton tyrosine kinase (BTK) inhibitors (BTKi) is attributed to acquired BTK/phospholipase C gamma 2 (PLCG2) mutations. However, knowledge regarding additional genetic lesions associated with BTK/PLCG2 mutations, and gene mutations in patients lacking BTK/PLCG2 mutations, is limited.

Methods: Using targeted deep sequencing, mutations in 29 genes associated with CLL and/or the BCR signaling pathway were assessed in patients with CLL who developed resistance to BTK inhibition with ibrutinib/acalabrutinib at a single institution. Read More

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December 2018

The pequi pulp oil (Caryocar brasiliense Camb.) provides protection against aging-related anemia, inflammation and oxidative stress in Swiss mice, especially in females.

Genet Mol Biol 2018 Nov 29. Epub 2018 Nov 29.

Departmento de Genética e Morfologia, Instituto de Ciências Biologicas, Universidade de Brasília, Brasilia, DF, Brazil.

Continued exposure to reactive oxygen species and inflammation are the rationale behind aging theories and associated diseases. Scientific evidence corroborates the ethnomedicinal use of the oil of pequi (Caryocar brasiliense Camb.), a typical Brazilian Cerrado fruit, against oxidative damage to biomolecules and inflammation. Read More

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November 2018
5 Reads

Label-Free Identification of Lymphocyte Subtypes Using Three-Dimensional Quantitative Phase Imaging and Machine Learning.

J Vis Exp 2018 Nov 19(141). Epub 2018 Nov 19.

Department of Physics, Korea Advanced Institute of Science and Technology; KAIST Institute for Health Science and Technology, Korea Advanced Institute of Science and Technology; Tomocube, Inc.;

We describe here a protocol for the label-free identification of lymphocyte subtypes using quantitative phase imaging and machine learning. Identification of lymphocyte subtypes is important for the study of immunology as well as diagnosis and treatment of various diseases. Currently, standard methods for classifying lymphocyte types rely on labeling specific membrane proteins via antigen-antibody reactions. Read More

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November 2018

Autologous canine immunotherapy: short-time generated dendritic cells loaded with canine transmissible venereal tumor-whole lysate.

Immunopharmacol Immunotoxicol 2018 Dec 3:1-7. Epub 2018 Dec 3.

a Virology and Immunology Laboratory, Biological Science Faculty , Autonomous University of Nuevo León (AUNL) , San Nicolás de los Garza , México.

Objective: The aim of the present study was to evaluate the therapeutic potential of autologous DCs loaded with whole tumor cell lysate of CTVT generated under a simplified and rapid procedure in vitro production process, in a vulvar submucosal model of CTVT in dogs.

Materials And Methods: We generated a model of intravulvar CTVT in dogs. A CTVT lysate antigen was prepared according to the method of 1-butanol and after administered with complete Freund's adjuvant via subcutaneous in female healthy dogs and challenge with CTVT cells to corroborate the immunogenicity. Read More

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December 2018
1 Read

Tumor Lysate-Loaded Lipid Hybrid Nanovaccine Collaborated with an Immune Checkpoint Antagonist for Combination Immunotherapy.

Adv Healthc Mater 2018 Dec 3:e1800837. Epub 2018 Dec 3.

Tongji School of Pharmacy, Huazhong University of Science and Technology, Wuhan, 430030, China.

Cancer vaccines have shown great potential for treating different types of cancer. However, the application of vaccination still presents two major challenges. One is efficiency of antigen delivery, and the other is dealing with immune tolerance accompanied with tumor development. Read More

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December 2018

Incidence of and risk factors for major haemorrhage in patients treated with ibrutinib: An integrated analysis.

Br J Haematol 2018 Dec 2. Epub 2018 Dec 2.

The University of Texas MD Anderson Cancer Center, Houston, TX, USA.

Ibrutinib, a Bruton tyrosine kinase inhibitor, is approved for treatment of various B-cell malignancies. In ibrutinib clinical studies, low-grade haemorrhage was common, whereas major haemorrhage (MH) was infrequent. We analysed the incidence of and risk factors for MH from 15 ibrutinib clinical studies (N = 1768), including 4 randomised controlled trials (RCTs). Read More

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December 2018

A benzoxazole derivative PO-296 inhibits T lymphocyte proliferation by the JAK3/STAT5 signal pathway.

J Cell Biochem 2018 Dec 2. Epub 2018 Dec 2.

Center of Science and Research, Chengdu Medical College, Chengdu, Sichuan, China.

Immunosuppressants have shown striking achievements in treating autoimmune diseases in recent years. It is urgent to develop more immunosuppressants to provide more options for patients. PO-296 [2-(6-chlorobenzo[d]oxazol-2-yl)-4,5,6,7-tetrahydro-2H-indazol-3-ol] was identified as a novel benzoxazole derivative. Read More

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December 2018

Comparison of 5 different scoring methods in the evaluation of inflammatory infiltration (tumor infiltrating lymphocytes - TILs) in superficial spreading and nodular melanoma.

Pigment Cell Melanoma Res 2018 Dec 1. Epub 2018 Dec 1.

Institute of Pathology, First Faculty of Medicine, Charles University and General University Hospital in Prague, Czech Republic.

The objective of our study is a comparison of five different scoring methods of tumor infiltrating lymphocytes (TILs) assessment in a group of 213 cases of superficial spreading and nodular melanoma. The scoring methods include i) Clark scoring; ii) Melanoma Institute Australia system; iii) scoring system used in the study of Saldanha et al.; iv) scoring system used in the TCGA study and modified by Park et al. Read More

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December 2018

The correlation and prognostic value of serum levels of soluble programmed death protein 1 (sPD-1) and soluble programmed death-ligand 1 (sPD-L1) in patients with hepatocellular carcinoma.

Cancer Immunol Immunother 2018 Dec 1. Epub 2018 Dec 1.

State Key Laboratory of Oncology in South China, Collaborative Innovation Center for Cancer Medicine, Sun Yat-sen University Cancer Center, Guangzhou, 510060, Guangdong, People's Republic of China.

Background: Blocking the programmed death protein 1 (PD-1)/programmed death-ligand 1 (PD-L1) pathway in hepatocellular carcinoma (HCC) is a very promising approach in immunotherapy. However, the correlation and prognostic values of serum soluble PD-1 and PD-L1 (sPD-1/sPD-L1) have not been explored conjointly in HCC patients.

Methods: This study retrospectively included 120 HCC patients receiving radical resection. Read More

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December 2018
4 Reads

Cytotoxic and genotoxic effects induced by enrofloxacin-based antibiotic formulation Floxagen in two experimental models of bovine cells in vitro: peripheral lymphocytes and cumulus cells.

Environ Sci Pollut Res Int 2018 Nov 30. Epub 2018 Nov 30.

IGEVET, Instituto de Genética Veterinaria "Ing. Fernando N Dulout" (UNLP-CONICET-CONICET LA PLATA), Facultad de Ciencias Veterinarias - UNLP, Universidad Nacional de La Plata - CONICET, Calle 60 y 118, B1904AMA, La Plata, Buenos Aires, Argentina.

The in vitro effect of enrofloxacin (EFZ) was tested on two experimental somatic bovine cells in vitro: peripheral lymphocytes (PLs) and cumulus cells (CCs). The cytotoxicity and genotoxicity of this veterinary antibiotic were assessed using 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assays, single-cell gel electrophoresis (SCGE) assay, and cytokinesis-block micronucleus cytome (CBMN cyt) assay. Cells were treated during 24 h, and three concentrations were tested (50 μg/mL, 100 μg/mL, 150 μg/mL). Read More

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November 2018

Immunological status of chronically exposed persons with increased level of TCR mutations.

Radiat Environ Biophys 2018 Dec 1. Epub 2018 Dec 1.

South Ural State Medical University, Chelyabinsk, Russia.

Dose- and dose rate-dependent increase in the number of СD3СD16CD56-lymphocytes and lysosomal activity of neutrophils was noted in peripheral blood of the Techa riverside residents with increased level of TCR gene-mutated T lymphocytes long after chronic exposure of predominantly red bone marrow (mean exposure dose was 0.89 ± 0.09 Gy, individual dose range was 0. Read More

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December 2018

Immunological modulation following bone marrow-derived mesenchymal stromal cells and Th17 lymphocyte co-cultures.

Inflamm Res 2018 Nov 30. Epub 2018 Nov 30.

Osteoarthritis Research Unit, Department of Medicine, University of Montreal Hospital Research Center (CRCHUM), 900 rue Saint-Denis, R11.424, Montreal, QC, H2X 0A9, Canada.

Objective And Design: The objective of the study is to uncover the influence of human bone marrow-derived mesenchymal stem cells (BM-MSCs) on the generation of Th17 lymphocytes in co-cultures of both BM-MSCs and T cells.

Materials And Methods: BM-MSCs, characterized according to the international society for cellular therapy (ISCT) criteria, were co-cultured with T cells isolated from peripheral blood. The expression levels of IL-17 receptor, RORγt and IL-23 receptor were evaluated using flow cytometry. Read More

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November 2018

Cytologic Analysis of Epstein-Barr Virus-Associated T/Natural Killer-Cell Lymphoproliferative Diseases.

Authors:
Akihiro Yachie

Front Pediatr 2018 16;6:327. Epub 2018 Nov 16.

Department of Pediatrics, School of Medicine, Graduate School of Medical Sciences, Kanazawa University, Kanazawa, Japan.

Rapid, precise diagnosis of Epstein-Barr virus-associated T lymphocyte or natural killer cell lymphoproliferative diseases is clinically important to prevent disease progression and avoid fatal outcomes for patients. In addition to detecting increased copy numbers of Epstein-Barr virus, identification of the lymphocyte subpopulation targeted by the virus infection is crucial to reaching the final diagnosis. However, these procedures are laborious and require large amounts of sample. Read More

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November 2018
1 Read

Functional Heterogeneity of CD4 Tumor-Infiltrating Lymphocytes With a Resident Memory Phenotype in NSCLC.

Front Immunol 2018 16;9:2654. Epub 2018 Nov 16.

Sanquin Research, Department of Hematopoiesis, and Landsteiner Laboratory, Amsterdam UMC, University of Amsterdam, Amsterdam, Netherlands.

Resident memory T cells (T) inhabit peripheral tissues and are critical for protection against localized infections. Recently, it has become evident that CD103 T are not only important in combating secondary infections, but also for the elimination of tumor cells. In several solid cancers, intratumoral CD103CD8 tumor infiltrating lymphocytes (TILs), with T properties, are a positive prognostic marker. Read More

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November 2018

The assessment of radio-adaptive response in graves' hyperthyroidism patients following radioactive iodine uptake.

World J Nucl Med 2018 Oct-Dec;17(4):270-274

Department of Medical Physics Radiobiology and Radiation Protection, Babol University of Medical Sciences, Babol, Iran.

Low doses of radiation affect the response of cells to higher doses; this phenomenon is called radio-adaptive response, which leads to increased resistance to subsequent higher doses. We have investigated the adaptive response using 0.37 MBq priming dose of I-131 followed by 296-444 MBq challenging dose in peripheral human lymphocyte cells. Read More

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December 2018

The effect of and α-linolenic acid on oxidant/antioxidant biomarkers of human peripheral blood mononuclear cells.

Indian J Pharmacol 2018 Jul-Aug;50(4):177-184

Department of Basic Science, School of Veterinary, Shiraz University, Shiraz, Iran.

Objectives: Various pharmacological effects including antioxidant property of L. were reported previously. In the present study, the effect of the extract of the plant and its constituent, α-linolenic acid (ALA), on oxidant and antioxidant markers of PHA/non-stimulated human mononuclear cells was evaluated. Read More

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December 2018

Herpes simplex encephalitis and pseudotumour cerebri due to ısotretinoin.

J Pak Med Assoc 2018 Dec;68(12):1833-11835

Department of Internal Medicine, Medical School, Firat University, Elazig Turkey.

Isotretinoin can have significant adverse reactions on multiple systems. We report a unique case of pseudotumour cerebri and herpes encephalitis resulting due to isotretinoin. A 19-year-old female patient was admitted to our clinic, presenting with headache, nausea, vomiting and fever since three days. Read More

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December 2018
4 Reads

Human preprocalcitonin self-antigen generates TAP-dependent and -independent epitopes triggering optimised T-cell responses toward immune-escaped tumours.

Nat Commun 2018 Nov 30;9(1):5097. Epub 2018 Nov 30.

INSERM UMR 1186, Integrative Tumour Immunology and Genetic Oncology, Gustave Roussy, EPHE, PSL, Fac. de Médecine, Univ. Paris-Sud, Université Paris-Saclay, 94805, Villejuif, France.

Tumours often evade CD8 T-cell immunity by downregulating TAP. T-cell epitopes associated with impaired peptide processing are immunogenic non-mutated neoantigens that emerge during tumour immune evasion. The preprocalcitonin (ppCT) neoepitope belongs to this category of antigens. Read More

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November 2018
1 Read

A Rare Mosaic Karyotype of 45,X/46,X,psu idic(Y)(p11.32)/46,XY with SHOX Haploinsufficiency, External Male Genitalia, and Short Stature.

Sex Dev 2018 Dec 1. Epub 2018 Dec 1.

In this case study, we describe a 3-year-old boy who was referred to the Inonu University Hospital with short stature complaint. His height was 86 cm (-2.96 SDS), weight was 12 kg (-2. Read More

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December 2018
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