20,081 results match your criteria Listeria Monocytogenes


Transbilayer Movement of Sphingomyelin Precedes Catastrophic Breakage of Enterobacteria-Containing Vacuoles.

Curr Biol 2020 Jun 26. Epub 2020 Jun 26.

MRC Laboratory of Molecular Biology, Division of Protein and Nucleic Acid Chemistry, Francis Crick Avenue, Cambridge CB2 0QH, UK; University of Cambridge, Department of Medicine, Addenbrooke's Hospital, Cambridge CB2 0QQ, UK. Electronic address:

Pathogenic bacteria enter the cytosol of host cells through uptake into bacteria-containing vacuoles (BCVs) and subsequent rupture of the vacuolar membrane [1]. Bacterial invaders are sensed either directly, through cytosolic pattern-recognition receptors specific for bacterial ligands, or indirectly, through danger receptors that bind host molecules displayed in an abnormal context, for example, glycans on damaged BCVs [2-4]. In contrast to damage caused by Listeria monocytogenes, a Gram-positive bacterium, BCV rupture by Gram-negative pathogens such as Shigella flexneri or Salmonella Typhimurium remains incompletely understood [5, 6]. Read More

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http://dx.doi.org/10.1016/j.cub.2020.05.083DOI Listing

Periprosthetic Joint Infection with Listeria monocytogenes: A Case Report.

JBJS Case Connect 2020 Apr-Jun;10(2):e1900489

1Rothman Orthopaedic Institute at Thomas Jefferson University, Philadelphia, Pennsylvania.

Case: A 67-year-old immunocompromised woman presented with 2 weeks of atraumatic knee pain 4 months after a primary total knee arthroplasty. The patient had a history of consuming unpasteurized dairy products and horse rearing. The patient underwent a successful irrigation, debridement, polyethylene liner exchange, and adjunct antibiotic therapy for a periprosthetic joint infection (PJI). Read More

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http://dx.doi.org/10.2106/JBJS.CC.19.00489DOI Listing

Inactivation of Listeria monocytogenes and Salmonella Typhimurium in strawberry juice enriched with strawberry polyphenols.

J Sci Food Agric 2020 Jul 10. Epub 2020 Jul 10.

Institute of Microbiology, Faculty of Biochemistry, Chemistry and Pharmacy (FBQF), National University of Tucumán (UNT).

Background: To extract and identify the low molecular weight phenolic fractions (LMPFs) from Albion (LMPF-A) and Camarosa (LMPF-C) strawberry cultivars, investigate their antibacterial activities against Listeria monocytogenes and Salmonella Typhimurium cocktails in vitro and in vivo using strawberry juice as food model; and also determine the antibacterial mechanism.

Results: Quercetin was identified as a principal compound in both phenolic fractions and their MIC and MBC values were around 600 and 950 μg mL against S. Typhimurium and L. Read More

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http://dx.doi.org/10.1002/jsfa.10653DOI Listing

Th1 responses in vivo require cell-specific provision of OX40L dictated by environmental cues.

Nat Commun 2020 Jul 9;11(1):3421. Epub 2020 Jul 9.

Institute of Immunology and Immunotherapy, College of Medical and Dental Sciences, University of Birmingham, Birmingham, UK.

The OX40-OX40L pathway provides crucial co-stimulatory signals for CD4 T cell responses, however the precise cellular interactions critical for OX40L provision in vivo and when these occur, remains unclear. Here, we demonstrate that provision of OX40L by dendritic cells (DCs), but not T cells, B cells nor group 3 innate lymphoid cells (ILC3s), is critical specifically for the effector Th1 response to an acute systemic infection with Listeria monocytogenes (Lm). OX40L expression by DCs is regulated by cross-talk with NK cells, with IFNγ signalling to the DC to enhance OX40L in a mechanism conserved in both mouse and human DCs. Read More

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http://dx.doi.org/10.1038/s41467-020-17293-3DOI Listing

Response surface optimization of phenolic compounds extraction from camu-camu (Myrciaria dubia) seed coat based on chemical properties and bioactivity.

J Food Sci 2020 Jul 9. Epub 2020 Jul 9.

Department of Food Engineering, State University of Ponta Grossa, Ponta Grossa, Brazil.

Food companies should comply with the requirements of a zero-waste concept to adapt to the circular economy requirements. In fruit companies, usually seeds are discarded without proper utilization and extraction of the bioactive compounds. Fruit seeds are sources of chemical compounds that can be extracted, studied, and applied in high value-added products. Read More

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http://dx.doi.org/10.1111/1750-3841.15327DOI Listing

Acute neonatal Listeria monocytogenes infection causes long-term, organ-specific changes in immune cell subset composition.

Eur J Microbiol Immunol (Bp) 2020 Jun 19. Epub 2020 Jun 19.

1Department Experimental Immunology, Helmholtz Centre for Infection Research, Braunschweig, Germany.

Listeria monocytogenes (Lm) is a food-borne pathogen with a high chance of infecting neonates, pregnant women, elderly and immunocompromised individuals. Lm infection in neonates can cause neonatal meningitis and sepsis with a high risk of severe neurological and developmental sequelae and high mortality rates. However, whether an acute neonatal Lm infection causes long-term effects on the immune system persisting until adulthood has not been fully elucidated. Read More

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http://dx.doi.org/10.1556/1886.2020.00007DOI Listing

Endogenous Endophthalmitis Caused by Isolated Listeria Monocytogenes Infection.

Ocul Immunol Inflamm 2020 Jul 9:1-5. Epub 2020 Jul 9.

Department of Ophthalmology, University Hospitals Leuven , Leuven, Belgium.

Purpose: The purpose of this report is to present an extremely rare case of endogenous Listeria monocytogenes endophthalmitis.

Methods: A 72-year-old woman was diagnosed with endogenous endophthalmitis. Listeria monocytogenes was identified by culture of aqueous humor. Read More

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http://dx.doi.org/10.1080/09273948.2020.1769140DOI Listing

Role of base excision repair in Listeria monocytogenes DNA stress survival during infections.

J Infect Dis 2020 Jul 9. Epub 2020 Jul 9.

Department of Microbiology and Parasitology, School of Medicine, Zhejiang University, Hangzhou, China.

Background: Base excision repair (BER), consisting mostly of lesion-specific DNA glycosylases and apurinic/apyrimidinic (AP) endonucleases, is one of the most important DNA repair mechanisms for repair of single nucleobase lesions generated by reactive oxygen and nitrogen species as part of an immune response against bacterial infections. However, few studies have addressed the contribution of BER to bacterial virulence and Listeria monocytogenes BER has thus far remained completely uncharacterized.

Methods: Analysis of the L. Read More

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http://dx.doi.org/10.1093/infdis/jiaa412DOI Listing

Saccharothrix algeriensis NRRL B-24137, the first non-Streptomyces actinobacterium, produces holomycin after cystine feeding.

Arch Microbiol 2020 Jul 8. Epub 2020 Jul 8.

Laboratoire de Biologie des Systèmes Microbiens (LBSM), Ecole Normale Supérieure de Kouba, Alger, Algeria.

Saccharothrix algeriensis NRRL B-24137 is an actinobacterium isolated from Algerian Saharan soil. This strain has the ability to produce several dithiolopyrrolone antibiotic derivatives depending on the precursors added to the culture medium. This group of antibiotics is known for their potent antimicrobial and anticancer activities. Read More

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http://dx.doi.org/10.1007/s00203-020-01971-3DOI Listing

Enzymatic characterization and structure-function relationship of two chitinases, LmChiA and LmChiB, from .

Heliyon 2020 Jul 1;6(7):e04252. Epub 2020 Jul 1.

Graduate Program in Biomedical Sciences, Faculty of Allied Health Sciences, Thammasat University, Pathum Thani, Thailand.

possesses two chitinases (LmChiA and LmChiB) belonging to glycoside hydrolase family 18 (GH18). In this study, two chitinase genes ( and ) from 10403S were cloned and their biochemical characteristics were studied. Using colloidal chitin as substrate, both chitinases exhibited maximum catalytic activity at pH 6-7 with optimum temperature at 50 °C. Read More

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http://dx.doi.org/10.1016/j.heliyon.2020.e04252DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7334433PMC

Microbiological Quality of Cooked Chicken: Results of Monitoring in England (2013-17).

J Food Prot 2020 Jul 7. Epub 2020 Jul 7.

Public Health England Colindale 61 Colindale Ave UNITED KINGDOM London NW9 5HT.

Results from monitoring of the microbiological quality of 2,721 samples of ready-to-eat cooked chicken collected between 2013 to 2017 in England were reviewed: 70% of samples were from retail, catering or manufacture and 30% were imported and collected at English ports. Samples were tested for a range of bacterial pathogens and indicator organisms. Six samples (<1%) had unsatisfactory levels of pathogens which were potentially injurious to health. Read More

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http://dx.doi.org/10.4315/JFP-20-187DOI Listing

Effectiveness of a Novel, Rechargeable, Polycationic N-halamine Antibacterial Coating on Listeria Monocytogenes Survival in Food Processing Environments.

J Food Prot 2020 Jul 7. Epub 2020 Jul 7.

University of Manitoba Elizabeth Dafoe Library Associate Professor Food and Human Nutritional Sciences 13 Freedman crescent CANADA Winnipeg Manitoba R3T 2N2 2044746658.

The goal of this research was to evaluate the efficacy of a novel rechargeable non-leaching polycationic N-halamine coating applied to stainless steel (STEEL) food contact surfaces to reduce Listeria monocytogenes contamination on ready-to-eat (RTE) foods. For this purpose, four L. monocytogenes strains were inoculated onto the charged (C; chlorine-activated) or uncharged (NC) N-halamine coated STEEL coupon surfaces (intact vs. Read More

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http://dx.doi.org/10.4315/JFP-20-084DOI Listing

TLR2 and endosomal TLR-mediated secretion of IL-10 and immune suppression in response to phagosome-confined Listeria monocytogenes.

PLoS Pathog 2020 Jul 7;16(7):e1008622. Epub 2020 Jul 7.

Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, California, United States of America.

Listeria monocytogenes is a facultative intracellular bacterial pathogen that escapes from phagosomes and induces a robust adaptive immune response in mice, while mutants unable to escape phagosomes fail to induce a robust adaptive immune response and suppress the immunity to wildtype bacteria when co-administered. The capacity to suppress immunity can be reversed by blocking IL-10. In this study, we sought to understand the host receptors that lead to secretion of IL-10 in response to phagosome-confined L. Read More

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http://dx.doi.org/10.1371/journal.ppat.1008622DOI Listing

Force-Averaging DLVO Model Predictions of the Adhesion Strengths Quantified for Pathogenic Listeria monocytogenes EGDe Grown under Variable pH Stresses.

Langmuir 2020 Jul 7. Epub 2020 Jul 7.

The roles of the bacterial surface biopolymers of pathogenic Listeria monocytogenes EGDe grown at variable pH conditions in governing their adhesion to a model surface of silicon nitride were investigated using atomic force microscopy (AFM) under water. Our results indicated that the adhesion forces were the highest for cells cultured in media adjusted to pH 7, followed by a 1.39, 1. Read More

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http://dx.doi.org/10.1021/acs.langmuir.0c01500DOI Listing

Assessment of the Antimicrobial, Antioxidant, and Antiproliferative Potential of subps. Essential Oil.

Foods 2020 Jul 1;9(7). Epub 2020 Jul 1.

Laboratory of Applied Microbiology and Biotechnology, Department of Molecular Biology & Genetics, Democritus University of Thrace, GR-68100 Alexandroupolis, Greece.

The aim of the present study was to investigate the antimicrobial potential of subps. essential oil (EO) against common food spoilage and pathogenic microorganisms and evaluate its antioxidant and antiproliferative activity. The EO was isolated by steam distillation and analyzed by GC/MS. Read More

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http://dx.doi.org/10.3390/foods9070860DOI Listing

Antimicrobial Activity of Five Apitoxins from on Two Common Foodborne Pathogens.

Antibiotics (Basel) 2020 Jun 30;9(7). Epub 2020 Jun 30.

Laboratorio de Higiene Inspección y Control de Alimentos, Departamento de Química Analítica, Nutrición y Bromatología, Universidad de Santiago de Compostela, 27002 Lugo, Spain.

Antimicrobial resistance is one of today's major public health challenges. Infections caused by multidrug-resistant bacteria have been responsible for an increasing number of deaths in recent decades. These resistant bacteria are also a concern in the food chain, as bacteria can resist common biocides used in the food industry and reach consumers. Read More

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http://dx.doi.org/10.3390/antibiotics9070367DOI Listing

Virulence, Antimicrobial Resistance and Environmental Persistence: A Review.

Pathogens 2020 Jun 30;9(7). Epub 2020 Jun 30.

SAMRC Microbial Water Quality Monitoring Centre, University of Fort Hare, Alice 5700, South Africa.

is a ubiquitous opportunistic pathogen responsible for the well-known listeriosis disease. This bacterium has become a common contaminant of food, threatening the food processing industry. Once consumed, the pathogen is capable of traversing epithelial barriers, cellular invasion, and intracellular replication through the modulation of virulence factors such as internalins and haemolysins. Read More

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http://dx.doi.org/10.3390/pathogens9070528DOI Listing

Adsorption of plantaricin Q7 on montmorillonite and application in feedback regulation of plantaricin Q7 synthesis by Q7.

Eng Life Sci 2019 Jan 15;19(1):57-65. Epub 2018 Nov 15.

School of Chemistry and Chemical Engineering Harbin Institute of Technology Harbin Heilongjiang P. R. China.

Kieselguhr, bentonite, and montmorillonite were investigated as potential carriers of plantaricin Q7. Highest level of adsorption of plantaricin Q7 was obtained with montmorillonite. Meanwhile, visible inhibition zones were observed against for montmorillonite adsorbed with plantaricin Q7. Read More

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http://dx.doi.org/10.1002/elsc.201800086DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6999430PMC
January 2019

Risk-Benefit Assessment of Foods.

EFSA J 2019 Sep 17;17(Suppl 2):e170917. Epub 2019 Sep 17.

Food is an elementary requirement for human life, providing nutrients and essential energy needed for optimal health. But at the same time, food can also be a vehicle of hazardous substances or pathogens that could affect human health negatively. Risk-benefit assessment (RBA) of foods, a relatively new methodology for decision support, integrates nutrition, toxicology, microbiology, chemistry and human epidemiology for a comprehensive health impact assessment. Read More

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http://dx.doi.org/10.2903/j.efsa.2019.e170917DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7015517PMC
September 2019

Quantitative risk assessment of  in a traditional RTE product.

EFSA J 2019 Sep 17;17(Suppl 2):e170906. Epub 2019 Sep 17.

Ready to Eat (RTE) cooked meat products are among the most consumed RTE food subcategories in the EU. They are also associated with the highest number of listeriosis cases per year. Contamination with may arise from post-processing and its growth is often supported by the pH and water activity of the product. Read More

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http://dx.doi.org/10.2903/j.efsa.2019.e170906DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7015512PMC
September 2019

Draft Genome Sequence of Listeria monocytogenes Serovar 1/2a Strain IZSAM_Lm_14-16064, Isolated from an Italian Cooked Ham in 2014.

Microbiol Resour Announc 2020 Jul 2;9(27). Epub 2020 Jul 2.

National Reference Laboratory for Listeria monocytogenes, Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise G. Caporale, Teramo, Italy.

In this report, the draft genome sequence of serovar 1/2a strain IZSAM_Lm_14-16064, isolated in Italy from a cooked ham, is announced. The genome is similar to that of a clinical strain isolated in 2014. Read More

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http://dx.doi.org/10.1128/MRA.00558-20DOI Listing

Effect of dietary Lactobacilli mixture on Listeria monocytogenes infection and virulence property in broilers.

Poult Sci 2020 Jul 24;99(7):3655-3662. Epub 2020 Apr 24.

Department of Animal Production, Henan University of Science and Technology, Luoyang 471023, China; National Engineering Research Center of Biological Feed, Beijing 100008, China. Electronic address:

The present study aimed to investigate the effect of probiotic Lactobacilli addition on Listeria monocytogenes load, inflammatory reaction, and virulence properties in broilers from 1 to 14 D of age. A total of 480 broiler chicks were randomly allocated to 4 treatments of 6 replicates each. All birds were infected with L. Read More

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http://dx.doi.org/10.1016/j.psj.2020.03.058DOI Listing

Genomic characterization of the most barotolerant Listeria monocytogenes RO15 strain compared to reference strains used to evaluate food high pressure processing.

BMC Genomics 2020 Jul 2;21(1):455. Epub 2020 Jul 2.

Institute of Biotechnology, University of Helsinki, Helsinki, Finland.

Background: High pressure processing (HPP; i.e. 100-600 MPa pressure depending on product) is a non-thermal preservation technique adopted by the food industry to decrease significantly foodborne pathogens, including Listeria monocytogenes, from food. Read More

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http://dx.doi.org/10.1186/s12864-020-06819-0DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7331262PMC

Distribution and Diversity of Glycocin Biosynthesis Gene Clusters Beyond Firmicutes.

Glycobiology 2020 Jul 2. Epub 2020 Jul 2.

CSIR-Institute of Microbial Technology, Sector 39A, Chandigarh 160036, India.

Glycocins are the ribosomally synthesized glycosylated bacteriocins discovered and characterized in firmicutes, only. These peptides have antimicrobial activity against several pathogenic bacteria, including Streptococcus pyrogenes, Methicillin-resistant Staphylococcus aureus and food-spoilage bacteria Listeria monocytogenes. Glycocins exhibit immunostimulatory properties and make a promising source of new-antibiotics and food preservatives akin to Nisin. Read More

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http://dx.doi.org/10.1093/glycob/cwaa061DOI Listing

Borderline resistance to oxacillin in after treatment with sub-lethal sodium hypochlorite concentrations.

Heliyon 2020 Jun 21;6(6):e04070. Epub 2020 Jun 21.

Institute of Animal Hygiene and Veterinary Public Health, University of Leipzig, Leipzig, Germany.

Surface disinfectants are regularly used in prophylactic and infection control measures. Concern has been raised whether residues of sub-inhibitory disinfectant concentrations may constitute a selective pressure and could contribute to the development of strains which are tolerant and/or resistant to biocides including antibiotics. The current study investigated whether (. Read More

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http://dx.doi.org/10.1016/j.heliyon.2020.e04070DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7317233PMC

Estimating Listeria monocytogenes Growth in Ready-to-Eat Chicken Salad Using a Challenge Test for Quantitative Microbial Risk Assessment.

Risk Anal 2020 Jul 1. Epub 2020 Jul 1.

CIISA - Centro de Investigação Interdisciplinar em Sanidade Animal, Faculdade de Medicina Veterinária, Universidade de Lisboa, Avenida da Universidade Técnica, Lisboa, 1300-477, Portugal.

Currently, there is a growing preference for convenience food products, such as ready-to-eat (RTE) foods, associated with long refrigerated shelf-lives, not requiring a heat treatment prior to consumption. Because Listeria monocytogenes is able to grow at refrigeration temperatures, inconsistent temperatures during production, distribution, and at consumer's household may allow for the pathogen to thrive, reaching unsafe limits. L. Read More

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http://dx.doi.org/10.1111/risa.13546DOI Listing

Listeria monocytogenes prevalence varies more within fields than between fields or over time on conventionally farmed New York produce fields.

J Food Prot 2020 Jul 1. Epub 2020 Jul 1.

University of Rochester.

Past studies have shown that the on-farm distribution of Listeria monocytogenes is affected by environmental factors (e.g., weather). Read More

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http://dx.doi.org/10.4315/JFP-20-120DOI Listing

Antibacterial Activity of Four Plant Extracts Extracted from Traditional Chinese Medicinal Plants against and subsp. serovar Enteritidis.

Microorganisms 2020 Jun 26;8(6). Epub 2020 Jun 26.

Queen's University Belfast, Institute for Global Food Security, Belfast BT9 5DL, Northern Ireland, UK.

The worldwide ethnobotanical use of four investigated plants indicates antibacterial properties. The aim of this study was to screen and determine significant antibacterial activity of four plant extracts in vitro and in a poultry digest model. Using broth microdilution, the concentrations at which four plant extracts inhibited and over 24 hours was determined. Read More

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http://dx.doi.org/10.3390/microorganisms8060962DOI Listing

Listeria monocytogenes growth kinetics in refrigerated ready-to-eat dips and dip components.

PLoS One 2020 30;15(6):e0235472. Epub 2020 Jun 30.

Division of Food Processing Science and Technology, U. S. Food and Drug Administration, Bedford Park, Illinois, United States of America.

Refrigerated ready-to-eat (RTE) dips often have pH and water activity combinations conducive to the proliferation of foodborne pathogens, including Listeria monocytogenes. This study conducted product assessments of five refrigerated RTE dips: baba ghanoush, guacamole, hummus, pesto, and tahini, along with individual dip components including avocado, basil, chickpeas, cilantro, eggplant, garlic, and jalapeno pepper. Dips and dip components were inoculated with 2 log CFU/g of L. Read More

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http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0235472PLOS

Chemiluminescence assay for Listeria monocytogenes based on Cu/Co/Ni ternary nanocatalyst coupled with penicillin as generic capturing agent.

Luminescence 2020 Jun 30. Epub 2020 Jun 30.

Key Laboratory of Optic-electric Sensing and Analytical Chemistry for Life Science, MOE; Shandong Key Laboratory of Biochemical Analysis; Key Laboratory of Analytical Chemistry for Life Science in Universities of Shandong; College of Marine Science and Biological Engineering; Institute of Life Sciences and Biomass Resources; College of Chemistry and Molecular Engineering; Qingdao University of Science and Technology, Qingdao, China.

Bacterial pathogens control is important in seafood production. In this study, a Cu/Co/Ni ternary nanoalloy (Cu/Co/Ni TNA) is synthesized with oleylamine reducing method. It is found that Cu/Co/Ni TNA greatly enhances the chemiluminescence (CL) signal of hydroxylamine-O-sulfonicacid (HOSA)-luminol system. Read More

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http://dx.doi.org/10.1002/bio.3908DOI Listing

Short communication: Antimicrobial potential of Lactobacillus plantarum strains isolated from Slovak raw sheep milk cheeses.

J Dairy Sci 2020 Jun 26. Epub 2020 Jun 26.

Institute of Animal Physiology, Center of Biosciences of the Slovak Academy of Sciences, Šoltésovej 4-6, 040 01 Košice, Slovakia.

The objective of this study was to screen Lactobacillus plantarum strains isolated from the traditional Slovak raw sheep milk cheese for their inhibitory potential. Seventy-two strains were obtained from samples of raw sheep milk and raw sheep milk cheeses collected from April 2017 to September 2018, in 23 geographical areas of Eastern Slovakia, by inoculation of de Man, Rogosa, and Sharpe agar plates (Oxoid, Basingstoke, UK). Using both the genus- and species-specific PCR methods, 43 strains were identified as Lactobacillus spp. Read More

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http://dx.doi.org/10.3168/jds.2019-17862DOI Listing

Biological properties of sea buckthorn (Hippophae rhamnoides L.) derived products.

Acta Sci Pol Technol Aliment 2020 Apr-Jun;19(2):195-205

Department of Fruit Growing, Viticulture and Enology, Slovak University of Agriculture in Nitra, Slovakia.

Background: Sea buckthorn is a good and possible source of a wide range of bioactive compounds with     a positive effect on the human body, especially polyphenols and carotenoids.

Methods: The present study aimed to evaluate the antioxidant and antimicrobial activities of Hippophae rhamnoides L. products - 100% oil, 100% juice, dry berries, and tea (dry berries, leaves, and twigs). Read More

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http://dx.doi.org/10.17306/J.AFS.0809DOI Listing

[Listeriosis of the Central nervous system].

Ter Arkh 2019 Nov 15;91(11):38-44. Epub 2019 Nov 15.

Infectious clinical hospital №2.

Object: To study the main causes of severe course and high mortality in patients with nervous form of listeriosis.

Materials And Methods: The analysis of the course of Listeria meningoencephalitis (LM) in 36 patients aged from 9 to 85 years, who were treated in the Infectious clinical hospital No. 2 DZM (IKB No. Read More

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http://dx.doi.org/10.26442/00403660.2019.11.000360DOI Listing
November 2019

Synthesis and characterization of starch nanocellulosic films incorporated with Eucalyptus globulus leaf extract.

Int J Food Microbiol 2020 Jun 20;332:108765. Epub 2020 Jun 20.

Dr. S. S. Bhatnagar University Institute of Chemical Engineering & Technology, Panjab University, Chandigarh, India.

Carrot cellulose nano fibers (CNF) have been extracted from carrot pulp using acid hydrolysis process. The size of the CNF was in the range of 6.33-58. Read More

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http://dx.doi.org/10.1016/j.ijfoodmicro.2020.108765DOI Listing

Incorporation of apricot (Prunus armeniaca) kernel essential oil into chitosan films displaying antimicrobial effect against Listeria monocytogenes and improving quality indices of spiced beef.

Int J Biol Macromol 2020 Jun 25;162:838-844. Epub 2020 Jun 25.

Key Laboratory of Geospatial Technology for the Middle and Lower Yellow River Regions, Ministry of Education, Henan University, Kaifeng 475004, China.

The spiced beef is exposed to the invading of spoilage bacteria including Listeria monocytogenes. In the present study, the antimicrobial effects against L. monocytogenes of the chitosan (CS) films incorporated with apricot (Prunus armeniaca) kernel essential oil (AKEO) at 0. Read More

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http://dx.doi.org/10.1016/j.ijbiomac.2020.06.220DOI Listing

Differential modulation of fitness, virulence and transcription of virulence-associated genes in response to the presence of different microorganisms.

Appl Environ Microbiol 2020 Jun 26. Epub 2020 Jun 26.

Agricultural University of Athens, Department of Food Science and Human Nutrition, Laboratory of Food Quality Control and Hygiene, Iera odos 75, GR11855, Athens, Greece

Interactions between and food-associated or environmental bacteria are critical not only for the growth but also for a number of key biological processes of the microorganism. In this regard, limited information exists on the impact of other microorganisms on the virulence of In this study, the growth of was evaluated as single culture or in co-culture with and in tryptic soy broth (10°C/10 days and 37°C/24 hours). Transcriptional levels of 9 key virulence genes () and invasion efficiency and intracellular growth in Caco-2 cells, were determined for following grown in mono- or in co-culture for 3 days at 10°C or 9 hours at 37°C. Read More

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http://dx.doi.org/10.1128/AEM.01165-20DOI Listing

The sug operon involves in resistance to quaternary ammonium compounds in Listeria monocytogenes EGD-e.

Appl Microbiol Biotechnol 2020 Jun 25. Epub 2020 Jun 25.

Institute of Food Safety and Nutrition, Jinan University, Guangzhou, China.

In recent years, an increasing number of Listeria monocytogenes strains with resistance to quaternary ammonium compounds (QACs) have been reported. However, the genetic basis for QACs resistance in L. monocytogenes remains poorly understood. Read More

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http://dx.doi.org/10.1007/s00253-020-10741-6DOI Listing
June 2020
3.337 Impact Factor

Estimating deaths from foodborne disease in the UK for 11 key pathogens.

BMJ Open Gastroenterol 2020 Jun;7(1)

Information Team, West Suffolk NHS Foundation Trust, Bury St Edmunds, UK.

Objective: To estimate the number of deaths from foodborne disease in the UK from 11 key pathogens.

Design: Four different models were developed using data from a range of sources. These included enhanced surveillance, outbreaks, death certificates and hospital episode statistics data. Read More

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http://dx.doi.org/10.1136/bmjgast-2020-000377DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7319714PMC

Coordination of transcriptional and translational regulations in human epithelial cells infected by .

RNA Biol 2020 Jun 25:1-16. Epub 2020 Jun 25.

Bacterial Infection & RNA Destiny Group, Institut de biologie de l'ENS (IBENS), École normale supérieure, CNRS, INSERM, Université PSL , Paris, France.

The invasion of mammalian cells by intracellular bacterial pathogens reshuffles their gene expression and functions; however, we lack dynamic insight into the distinct control levels that shape the host response. Here, we have addressed the respective contribution of transcriptional and translational regulations during a time-course of infection of human intestinal epithelial cells by an epidemic strain of , using transcriptome analysis paralleled with ribosome profiling. Upregulations were dominated by early transcriptional activation of pro-inflammatory genes, whereas translation inhibition appeared as the major driver of downregulations. Read More

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http://dx.doi.org/10.1080/15476286.2020.1777380DOI Listing

Rapidly dynamic host cell heterogeneity in bacterial adhesion governs susceptibility to infection by .

Mol Biol Cell 2020 Jun 25:mbcE19080454. Epub 2020 Jun 25.

Department of Biology, University of Washington, Seattle, WA 98185-1800.

Interactions between host cells and individual pathogenic bacteria determine the clinical severity of disease during systemic infection in humans. Vascular endothelial cells, which line the lumen of blood vessels, represent a critical barrier for a bacterium in the bloodstream. These cells adopt myriad phenotypes that may modulate their susceptibility to infection, however the precise determinants of their heterogeneity in susceptibility are not known. Read More

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http://dx.doi.org/10.1091/mbc.E19-08-0454DOI Listing

A role for Taok2 in Listeria monocytogenes vacuolar escape.

J Infect Dis 2020 Jun 25. Epub 2020 Jun 25.

Unité de Recherche Yersinia, Institut Pasteur, Paris, France.

The bacterial pathogen Listeria monocytogenes (Lm) invades host cells, ruptures its internalization vacuole and reaches the cytosol for replication. A high-content siRNA microscopy screen allowed us to identify epithelial cell factors involved in Lm vacuolar rupture, among them the serine/threonine kinase Taok2. Kinase activity inhibition using a specific drug validated a role for Taok2 in favoring Lm cytoplasmic access. Read More

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http://dx.doi.org/10.1093/infdis/jiaa367DOI Listing

Listeriolysin O Pore-Forming Activity Is Required for ERK1/2 Phosphorylation During Infection.

Front Immunol 2020 3;11:1146. Epub 2020 Jun 3.

Key Laboratory of Applied Technology on Green-Eco-Healthy Animal Husbandry of Zhejiang Province, China-Australia Joint Laboratory for Animal Health Big Data Analytics, Zhejiang Provincial Engineering Laboratory for Animal Health Inspection & Internet Technology, College of Animal Science and Technology & College of Veterinary Medicine of Zhejiang A&F University, Hangzhou, China.

Listeriolysin O (LLO) is a cholesterol-dependent cytolysin that mediates escape of from phagosomes and enables the bacteria to grow within the host. LLO is a versatile tool allowing to trigger several cellular responses. In this study, rapid phosphorylation of ERK1/2 on Caco-2 cells caused by infection was demonstrated to be highly dependent on LLO activity. Read More

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http://dx.doi.org/10.3389/fimmu.2020.01146DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7283531PMC

Adjuvanting Allergen Extracts for Sublingual Immunotherapy: Calcitriol Downregulates CXCL8 Production in Primary Sublingual Epithelial Cells.

Front Immunol 2020 9;11:1033. Epub 2020 Jun 9.

Laboratory of Immunology, Department of Virology, Parasitology and Immunology, Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium.

Application of allergens onto the sublingual epithelium is used to desensitize allergic individuals, a treatment known as sublingual immunotherapy. However, the response of sublingual epithelial cells to house dust mite allergen and potential tolerance-promoting adjuvants such as Toll-like receptor (TLR) ligands and calcitriol has not been investigated. In order to study this, primary sublingual epithelial cells were isolated from dogs and cultured . Read More

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http://dx.doi.org/10.3389/fimmu.2020.01033DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7295906PMC

Application of yeast surface display system in expression of recombinant pediocin PA-1 in Saccharomyces cerevisiae.

Folia Microbiol (Praha) 2020 Jun 24. Epub 2020 Jun 24.

Department of Molecular and Environmental Biotechnology, Faculty of Biology and Biotechnology, University of Science, 227 Nguyen Van Cu St, Dist 5, Ho Chi Minh City, Vietnam.

Pediocin PA-1 is a bacteriocin that shows strongly anti-microbial activity against some Gram-positive pathogens such as Listeria monocytogenes, Staphylococcus aureus, and Enterococcus faecalis. With the broad inhibitory spectrum as well as high-temperature stability, pediocin has a potential application in the food preservation and pharmaceutical industry. Pediocin has been studied to express in many heterologous expression systems such as Escherichia coli, Saccharomyces cerevisiae, and Pichia pastoris as a free peptide. Read More

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http://dx.doi.org/10.1007/s12223-020-00804-6DOI Listing

Probiotic Properties of New Lactobacillus Strains Intended to Be Used as Feed Additives for Monogastric Animals.

Probiotics Antimicrob Proteins 2020 Jun 23. Epub 2020 Jun 23.

Institute of Fermentation Technology and Microbiology, Faculty of Biotechnology and Food Sciences, Lodz University of Technology, Wólczańska 171/173, 90-924, Łódź, Poland.

The study aimed to evaluate the safety and probiotic properties of selected Lactobacillus strains, which are intended to be fed to monogastric animals. The Lactobacillus spp. appeared to be safe since they did not degrade mucus and did not exhibit β-haemolysis. Read More

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http://dx.doi.org/10.1007/s12602-020-09674-3DOI Listing

Analysis of the Relationship of Microbial Contamination with Temperature and Cleaning Frequency and Method of Domestic Refrigerators in Japan.

J Food Prot 2020 Jul;83(7):1234-1240

Faculty of Agriculture, Kindai University, 3327-204, Nakamachi, Nara-shi, Nara 631-8505, Japan (ORCID: https://orcid.org/0000-0002-9651-9856 [M.M.]).

Abstract: Hygiene management of domestic refrigerators is an important aspect of food poisoning prevention. The aim of the present study was to confirm the relationship between microbial contamination and hygiene management by measuring microbial levels and investigating temperature and cleaning frequency and method of domestic refrigerators in Japan. We analyzed three internal sections (the egg compartment, bottom shelf, and vegetable drawer) of 100 domestic refrigerators in Japan. Read More

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http://dx.doi.org/10.4315/0362-028X.JFP-19-322DOI Listing

IκBNS and IL-6 expression is differentially established in the uterus of pregnant healthy and infected mice.

Heliyon 2020 Jun 13;6(6):e04122. Epub 2020 Jun 13.

Laboratorio de Inmunología Innata, Departamento de Inmunología, Escuela Nacional de Ciencias Biológicas - Instituto Politécnico Nacional (ENCB-IPN), Mexico City, Mexico.

During pregnancy, NF-κB plays an important role for embryo implantation and the onset of labor. Regulated IL-6 production, under transcriptional control of NF-κB, is essential for a successful pregnancy outcome and the atypical regulator IκBNS is involved in this process. Previously, we showed that IκBNS negatively regulates IL-6 in uterine tissues during mouse estrous cycle. Read More

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http://dx.doi.org/10.1016/j.heliyon.2020.e04122DOI Listing
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7301180PMC

The Role of Biofilms in the Development and Dissemination of Microbial Resistance within the Food Industry.

Foods 2020 Jun 21;9(6). Epub 2020 Jun 21.

Micalis Institute, INRAE, AgroParisTech, Université Paris-Saclay, 78350 Jouy-en-Josas, France.

Biofilms are multicellular sessile microbial communities embedded in hydrated extracellular polymeric matrices. Their formation is common in microbial life in most environments, while those formed on food-processing surfaces are of considerable interest in the context of food hygiene. Biofilm cells express properties that are distinct from planktonic ones, in particular, notorious resistance to antimicrobial agents. Read More

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http://dx.doi.org/10.3390/foods9060816DOI Listing

Interferes with Host Cell Mitosis through Its Virulence Factors InlC and ActA.

Toxins (Basel) 2020 Jun 20;12(6). Epub 2020 Jun 20.

Group of Molecular Microbiology, i3S-Instituto de Investigação e Inovação em Saúde, IBMC, Universidade do Porto, 4200-135 Porto, Portugal.

is among the best-characterized intracellular pathogens. Its virulence factors, and the way they interfere with host cells to hijack host functions and promote the establishment and dissemination of the infection, have been the focus of multiple studies over the last 30 years. During cellular infection, was shown to induce host DNA damage and delay the host cell cycle to its own benefit. Read More

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http://dx.doi.org/10.3390/toxins12060411DOI Listing

Antimicrobial resistance profile of Salmonella spp. and Listeria monocytogenes isolated from products marketed on the border of Brazil with Argentina and Uruguay.

J Food Prot 2020 Jun 23. Epub 2020 Jun 23.

Universidade Federal de Pelotas.

We aimed to perform serotyping and the antimicrobial resistance profile of Salmonella spp. and Listeria monocytogenes strains isolated from raw meats imported illegally into Brazil along the borders of Argentina and Uruguay. Distinct isolates of Salmonella spp. Read More

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http://dx.doi.org/10.4315/JFP-20-176DOI Listing