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Prev Nutr Food Sci 2019 Mar 31;24(1):70-74. Epub 2019 Mar 31.

Department of Animal Resource, Daegu University, Gyeongbuk 38453, Korea.

This study was conducted to investigate the antimicrobial activities of different solvent extracts of (MS) leaves against nine selected pathogenic microorganisms. The disc diffusion method was used to assess antimicrobial activity and determine minimum inhibitory concentrations (MIC). Methanol, and chloroform extracts showed significant inhibitory activity against and . Read More

Int J Food Microbiol 2019 Apr 5;300:31-42. Epub 2019 Apr 5.

Department of Food Science and Technology, Science and Research Branch, Islamic Azad University, P.O. Box 1476714171, Tehran, Iran.

Starch nanocrystal (S-NC) was produced after sulfuric acid hydrolysis of potato starch granules and then characterized by laser diffraction particle size analyzer, scanning electron microscopy (SEM) and X-ray diffraction (XRD). Response surface methodology (RSM) was applied to optimize S-NC (2-10%) concentration, sour lemon peel extract (SLPE, 2.5-12. Read More

J Glob Antimicrob Resist 2019 Apr 18. Epub 2019 Apr 18.

Departamento de Microbiologia Geral, Instituto de Microbiologia Paulo de Góes, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brasil. Electronic address:

Objectives: The aim of this study is to report the draft genome sequence of the bacteriocinogenic strain Enterococcus faecium E86. Bacteriocins are prokaryotic peptides or proteins with antimicrobial activity. The genome information may then contribute to the identification of the enterocins produced by this strain that exhibits inhibitory activity against the foodborne pathogen Listeria monocytogenes and vancomycin-resistant enterococci (VRE) involved in human infections, among other bacterial genera and species. Read More

Int J Biol Macromol 2019 Apr 17. Epub 2019 Apr 17.

Department of Chemical Sciences, University of Naples "Federico II", Complesso Universitario Monte S. Angelo, Via Cintia 4, 80126 Naples, Italy.

The probiotic features of strain GM newly isolated from Tunisian spontaneously fermented goat milk and identified as Bacillus tequilensis-GM were assessed. Strain GM showed high resistance to saliva (90.64%), gastric juice (88. Read More

AMB Express 2019 Apr 19;9(1):54. Epub 2019 Apr 19.

Department of Animal Production, College of Food and Agricultural Sciences, King Saud University, P.O Box 2460, Riyadh, 11451, Saudi Arabia.

The effects of packaging atmosphere, storage temperature and oregano essential oil (EO) on growth of Listeria monocytogenes on ready-to-eat smoked turkey were studied. Smoked turkey slices were inoculated with a strain of Listeria monocytogenes Scott A (5.95, 5. Read More

Microb Pathog 2019 Apr 16. Epub 2019 Apr 16.

Laboratory of Molecular Biology, Genomics and Bioinformatics, Faculty of Nature and Life Science, Hassiba Benbouali University, Chlef, Hay Essalam, 02000, Algeria.

A new exopolysaccharide (EPS) was produced by the Lactococcus lactis F-mou strain (LT898177.1) isolated from the Sahrawi camel milk in the Bir-Naam region, Algeria. The most influential production parameters were screened by the Plackett-Burman design for enhancing EPS yield utilizing the Mech-Degla juice as a low-cost raw material. Read More

J Appl Microbiol 2019 Apr 19. Epub 2019 Apr 19.

Medicinal and Natural Products Chemistry Research Center, Shiraz University of Medical Sciences, Department of Medicinal Chemistry, Faculty of Pharmacy, Shiraz, Iran.

Aims: The aim of this study was to develop a multiplex touchdown PCR (multiplex TD-PCR) for rapid and simultaneous detection of four major foodborne pathogens to avoid mispriming and unwanted production during gene amplification. Touchdown PCR is the modified form of standard PCR, which enhances specificity, sensitivity.

Methods And Results: For this reason, a multiplex TD-PCR assay with a pre-enrichment step was developed to detect four foodborne pathogens namely Escherichia coli O157:H7, Listeria monocytogenes, Staphylococcus aureus, and Salmonella enterica serovar Enteritidis in pure culture and raw milk samples. Read More

Food Res Int 2019 Jun 8;120:425-431. Epub 2019 Mar 8.

Department of Food Science and Technology, University of Georgia, 1109 Experiment Street, Griffin, GA 30223, USA. Electronic address:

Activated persulfate, a relatively new advanced oxidation process, has gained attention for its potential to ensure fresh produce safety. One of the major advantages is to avoid the formation of toxic chlorinated disinfection byproducts which are concerns for chlorine-based sanitizers. This study was aimed to investigate the efficacy of ferrous and alkaline activated persulfate in inactivating Escherichia coli O157:H7 and Listeria monocytogenes, with the primary focus on the effect of initial persulfate concentration, the effect of gradual addition of ferrous ion, and the stability of activated persulfate. Read More

Foodborne Pathog Dis 2019 Apr 18. Epub 2019 Apr 18.

1 Excellence Research Laboratory on Natural Products, Department of Microbiology, Faculty of Science and Natural Product Research Center of Excellence, Prince of Songkla University, Hat Yai, Thailand.

Consumer concerns toward chemical preservatives have resulted in increased search for healthy green alternative. In this study, the antioxidant activity and antibacterial effects of ethanolic leaf extract against a serious foodborne pathogen, was evaluated. Total phenolic and flavonoid contents of the extract were 11. Read More

Iran J Microbiol 2019 Feb;11(1):7-12

Department of Microbiology, Faculty of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.

Background And Objectives: Due to the widespread distribution of in environmental and animal sources and serious clinical complications in human, this study was aimed to isolate from water and clinical specimens by culture and PCR methods and to investigate the presence of and virulence genes.

Materials And Methods: Water and clinical samples of vaginal and fecal were screened for the presence of by phenotypic and standard biochemical tests. PCR amplification was performed on extracted DNA using primers based on the and genes. Read More

Proc Natl Acad Sci U S A 2019 Apr 17. Epub 2019 Apr 17.

Department of Biomedical Engineering, Columbia University, New York, NY 10027;

Synthetic biology is transforming therapeutic paradigms by engineering living cells and microbes to intelligently sense and respond to diseases including inflammation, infections, metabolic disorders, and cancer. However, the ability to rapidly engineer new therapies far outpaces the throughput of animal-based testing regimes, creating a major bottleneck for clinical translation. In vitro approaches to address this challenge have been limited in scalability and broad applicability. Read More

J Food Prot 2019 Apr 17:815-825. Epub 2019 Apr 17.

Department of Food Science and Technology, University of California, One Shields Avenue, Davis, California 95616, USA (ORCID: https://orcid.org/0000-0002-1911-752X [L.J.H.]).

During harvest, pistachios are hulled, separated in water into floater and sinker streams (in large part on the basis of nut density), and then dried before storage. Higher prevalence and levels of were previously observed in floater pistachios, but contributing factors are unclear. To examine the behavior of pathogens on hulled pistachios during simulated drying delays, floater and sinker pistachios collected from commercial processors were inoculated at 1 or 3 log CFU/g with cocktails of and in some cases O157:H7 or and incubated for up to 30 h at 37°C and 90% relative humidity. Read More

Foodborne Pathog Dis 2019 Apr 17. Epub 2019 Apr 17.

4 Department of Food Science, Fu Jen Catholic University, New Taipei City, Taiwan.

seeds contain many bioactive compounds commonly used in traditional Chinese medicine. In this study, the antibacterial activity and possible mechanism of seed ethanol extract (PCEE) against foodborne pathogens were investigated. Both methicillin-resistant (MRSA) and had similar minimum inhibitory concentrations and minimum bactericidal concentrations of PCEE at 50 and 100 μg/mL, respectively. Read More

BMC Infect Dis 2019 Apr 16;19(1):326. Epub 2019 Apr 16.

Bacteriology Laboratory, Department of Genetics and Laboratory Medicine, Geneva University Hospitals, rue Gabrielle Perret-Gentil 4, 1211, Geneva 14, Switzerland.

Background: Endograft infection is a rare but extremely dangerous complication of aortic repair (25-100% of mortality). We describe here the first case of Listeria monocytogenes abdominal periaortitis associated with a vascular graft. We also discuss the differential diagnosis of periaortitis and provide a literature review of L. Read More

Cells 2019 Apr 15;8(4). Epub 2019 Apr 15.

Paul-Ehrlich-Institut, Vice President´s Research Group 1: Molecular Allergology; 63225 Langen (Hesse), Germany.

Fusion proteins incorporating the TLR5-ligand flagellin are currently undergoing clinical trials as vaccine candidates for many diseases. We recently reported a flagellin:allergen fusion protein containing the TLR5-ligand flagellin A (FlaA) from and the major birch pollen allergen Bet v 1 (rFlaA:Betv1) to prevent allergic sensitization in an experimental mouse model. This study analyzes the signaling pathways contributing to rFlaA:Betv1-mediated pro- and anti-inflammatory cytokine secretion and cell metabolism in myeloid dendritic cells (mDCs) in vitro. Read More

Microb Biotechnol 2019 Apr 15. Epub 2019 Apr 15.

Molecular Food Microbiology Laboratory, Department of Food Science, Purdue University, West Lafayette, IN, USA.

Listeria monocytogenes has been implicated in a number of outbreaks including the recent largest outbreak in South Africa. Current methods for prevention of foodborne L. monocytogenes infection are inadequate, thus raising a need for an alternative strategy. Read More

J Appl Genet 2019 Apr 15. Epub 2019 Apr 15.

Food and Drug Department, University of Parma, Parco Area delle Scienze 49/A, Parma, Italy.

Over the last decade, several methods based on genomic DNA have been developed for the identification and genotyping of prokaryotic and eukaryotic organisms. These genomic methods differ regarding taxonomic range, discriminatory power, reproducibility, and ease of interpretation and standardization. The amplified fragment length polymorphism (AFLP) technique is a very powerful DNA fingerprinting technique for DNA of any source or complexity, varying in both size and base composition. Read More

J Antimicrob Chemother 2019 Apr 15. Epub 2019 Apr 15.

NHC Key Laboratory of Food Safety Risk Assessment, China National Center for Food Safety Risk Assessment, Beijing 100021, People's Republic of China.

Objectives: Our aim was to determine the antimicrobial susceptibilities of 2862 Listeria monocytogenes cultured from various foods in China and to use WGS to characterize the antimicrobial resistance and virulence genotypes of those expressing a resistance phenotype.

Methods: The susceptibilities of 2862 L. monocytogenes were determined by broth microdilution. Read More

J Bacteriol 2019 Apr 15. Epub 2019 Apr 15.

Department of Biochemistry and Molecular Biology, The Pennsylvania State University, University Park, Pennsylvania, USA

Bacterial lipoproteins are globular proteins anchored to the extracytoplasmic surfaces of cell membranes through lipidation at a conserved -terminal cysteine. Lipoproteins contribute to an array of important cellular functions for bacteria, as well as being a focal point for innate immune system recognition through binding to Toll-like receptor 2 (TLR2) heterodimer complexes. Although lipoproteins are conserved among nearly all classes of bacteria, the presence and type of α-amino-linked acyl chain is highly variable and even strain specific within a given bacterial species. Read More

Molecules 2019 Apr 3;24(7). Epub 2019 Apr 3.

School of Science, Anhui Agricultural University, Hefei 230036, China.

To develop new antibacterial agents, a series of novel triazole-containing pyrazole ester derivatives were designed and synthesized and their biological activities were evaluated as potential topoisomerase II inhibitors. Compound exhibited the most potent antibacterial activity with Minimum inhibitory concentration (MIC) alues of 4 µg/mL, 2 µg/mL, 4 µg/mL, and 0.5 µg/mL against , , , and , respectively. Read More

Microb Pathog 2019 Apr 12. Epub 2019 Apr 12.

School of Medical Instrument and Food Engineering, University of Shanghai for Science and Technology, Shanghai, 200093, PR China. Electronic address:

Live attenuated bacteria is a promising candidate vector for the delivery of vaccines in clinic trials. In the field of aquaculture industry, live vector vaccine also could provide long-term and effective protection against fish bacterial diseases. In our previous work, we demonstrated attenuated Listeria monocytogenes (Lm) had the potential to be an aquaculture vaccine vector in cellular level and zebrafish model. Read More

Front Microbiol 2019 28;10:612. Epub 2019 Mar 28.

Laboratorio de Microbiología y Probióticos, Instituto de Nutrición y Tecnología de los Alimentos (INTA), Universidad de Chile, Santiago, Chile.

is a foodborne pathogen that can cause severe disease in susceptible humans. This microorganism has the ability to adapt to hostile environmental conditions such as the low temperatures used by the food industry for controlling microorganisms. Bacteria are able to adjust their transcriptional response to adapt to stressful conditions in order to maintain cell homeostasis. Read More

Int J Food Microbiol 2019 Apr 2;300:1-7. Epub 2019 Apr 2.

Postgraduate Program in Agroindustrial Science and Technology, Agroindustrial Technology and Science Department, Eliseu Maciel Faculty of Agronomy, Federal University of Pelotas (UFPel), 96010-900, P.O. Box 354, Pelotas, RS, Brazil. Electronic address:

The objective of this study was to compare the gene expression levels of the agr locus and prfA gene during adhesion and biofilm formation by four L. monocytogenes isolates (2 biofilm-forming and 2 non-forming) on stainless steel and polystyrene surfaces at different temperatures (10 °C, 20 °C and 37 °C), and times (8 h, 12 h, 24 h and 48 h). The agrA and prfA genes were expressed at higher levels than the agrBCD genes. Read More

Meat Sci 2019 Apr 4;154:29-36. Epub 2019 Apr 4.

Laboratory of Microorganisms and Biomolecules (LMB), Centre of Biotechnology of Sfax, Road of Sidi Mansour Km 6, P.O. Box 1177, Sfax 3018, Tunisia. Electronic address:

The aim of this work was to evaluate the effect of different concentrations of probiotic strain Lactobacillus plantarum TN8 on the quality and safety of raw minced beef after 10 days of refrigerated storage. The obtained results showed that the incorporation of the probiotic strain can inhibit the proliferation of spoilage microorganisms, such Listeria monocytogenes and Salmonella spp., delay the lipid oxidation, improve texture parameters, and extend the shelf life of these products during storage. Read More

Biomedicines 2019 Apr 11;7(2). Epub 2019 Apr 11.

Gamaleya National Research Center of Epidemiology and Microbiology, 123098 Moscow, Russia.

HGF (hepatocyte growth factor)/HGFR (HGF receptor) signaling pathway is a key pathway in liver protection and regeneration after acute toxic damage. toxin InlB contains a HGFR-interacting domain and is a functional analog of HGF. The aim of this work was to evaluate the hepatoprotective activity of the InlB HGFR-interacting domain. Read More

Molecules 2019 Apr 11;24(7). Epub 2019 Apr 11.

Department of Life Science, Department of Energy Storage/Conversion Engineering of Graduate School, and Hydrogen and Fuel Cell Research Center, Chonbuk National University, Jeollabuk-do 54896, Korea.

1,4-Naphthoquinones have antibacterial activity and are a promising new class of compound that can be used to treat bacterial infections. The goal was to improve effective antibacterial agents; therefore, we synthesized a new class of naphthoquinone hybrids, which contain phenylamino-phenylthio moieties as significant counterparts. Compound was modified as a substituted aryl amide moiety, which enhanced the antibacterial activity of earlier compounds and . Read More

Int J Food Microbiol 2019 Mar 28;299:64-70. Epub 2019 Mar 28.

Department of Physiology, Biochemistry and Pharmacology, College of Veterinary Medicine, King Faisal University, Al-Ahsa 31982, Saudi Arabia.

Essential oils extracted by hydro-distillation form date palm spathe (byproduct from date palm plants) were tested for their antibacterial activity against some food-borne pathogens. Listeria monocytogenes ATCC 7644, Staphylococcus aureus ATCC 29243, Salmonella enterica subsp. enterica serovar Enteritidis ATCC 13076 and E. Read More

FEMS Microbiol Lett 2019 Apr 12. Epub 2019 Apr 12.

Department of Plant and Microbial Biology, North Carolina State University, Raleigh, NC 27695, USA.

Several outbreaks of listeriosis have implicated fresh produce but genetic factors required for growth of Listeria monocytogenes on produce remain poorly characterized. Based on the fact that β-lactam antibiotics only kill bacterial cells that are growing, we hypothesized that ampicillin selection can enrich for L. monocytogenes mutants unable to grow on produce. Read More

Braz J Microbiol 2019 Apr 11. Epub 2019 Apr 11.

Departamento de Biotecnología y Ciencias Alimentarias, Instituto Tecnológico de Sonora, 5 de Febrero 818 sur, Col. Centro, 85000, Ciudad Obregón, Sonora, Mexico.

This study focuses on the prevalence of Listeria monocytogenes (Lm) in pork meat and on inert surfaces from slaughterhouses in Sonora, Mexico. A total of 21 Lm were obtained from 103 samples, giving a prevalence of 20.3%. Read More

J Cell Biol 2019 Apr 11. Epub 2019 Apr 11.

Department of Biological Sciences, North Carolina State University, Raleigh, NC

RIPK3, a key mediator of necroptosis, has been implicated in the host defense against viral infection primary in immune cells. However, gene expression analysis revealed that RIPK3 is abundantly expressed not only in immune organs but also in the gastrointestinal tract, particularly in the small intestine. We found that orally inoculated , a bacterial foodborne pathogen, efficiently spread and caused systemic infection in -deficient mice while almost no dissemination was observed in wild-type mice. Read More

J Cell Physiol 2019 Apr 10. Epub 2019 Apr 10.

Department of Immunology, School of Basic Medical Sciences, Fudan University, Shanghai, China.

The metabolism of immune cells reprograms inflammatory responses to protect against infection by pathogenic microorganisms, but the immune effects of glycolysis and the oxidative phosphorylation (OXPHOS) metabolic pathway remain unclear. Herein, the effects of glycolysis or OXPHOS on the neutrophils and T cells were investigated using a pharmacological approach in mice. 2-Deoxy-d-glucose (2-DG), which blocks the key enzyme hexokinase of glycolysis, and dimethyl malonate (DMM), which blocks the key element succinate of OXPHOS, both efficiently expanded the population of neutrophils, but significantly inhibited tumor necrosis factor a secretion and reactive oxygen species (ROS) production. Read More

Front Microbiol 2019 26;10:563. Epub 2019 Mar 26.

Department of Agricultural and Food Sciences, Alma Mater Studiorum, University of Bologna, Cesena, Italy.

This work aimed to evaluate the potential of 15 nisin producing strains, isolated from dairy products, for the fermentation of soymilk and carrot juice. In particular, the acidification and the production of nisin in the food matrices were recorded. Moreover, three strains (LBG2, FBG1P, and 3LC39), that showed the most promising results were further scrutinized for their anti- activity and volatile molecules profile during fermentation of soymilk and carrot juice. Read More

PLoS One 2019 10;14(4):e0215017. Epub 2019 Apr 10.

Division of Biological Science and Technology, Yonsei University, Wonju, Republic of Korea.

Listeria monocytogenes is a foodborne pathogen that causes listeriosis and can lead to serious clinical problems, such as sepsis and meningitis, in immunocompromised patients and neonates. Due to a growing number of antibiotic-resistant L. monocytogenes strains, listeriosis can steadily become refractory to antibiotic treatment. Read More

Foodborne Pathog Dis 2019 Apr 10. Epub 2019 Apr 10.

1 Public Health Laboratories, Washington State Department of Health, Shoreline, Washington.

The Washington State Department of Health Public Health Laboratories (WAPHL) has tested 11,501 samples between 2007 and 2017 for a foodborne disease using a combination of identification, serotyping, and subtyping tools. During this period there were 8037 total clinical and environmental samples tested by pulsed-field gel electrophoresis (PFGE), including 512 foodborne disease clusters and 2176 PFGE patterns of Salmonella enterica subsp. enterica. Read More

Proteins 2019 Apr 10. Epub 2019 Apr 10.

High Pressure and Synchrotron Radiation Physics Division, Bhabha Atomic Research Centre, Mumbai, Maharashtra 400085, India.

Peptidase E (PepE) is a nonclassical serine peptidase with a Ser-His-Glu catalytic triad. It is specific for dipeptides with an N-terminal aspartate residue (Asp-X dipeptidase activity). Its homolog from Listeria monocytogenes (PepElm) has a Ser-His-Asn "catalytic triad". Read More

Int J Med Microbiol 2019 Mar 28. Epub 2019 Mar 28.

Department of Genetics, School of Biological Sciences, Madurai Kamaraj University, Madurai, 625021, Tamil Nadu, India. Electronic address:

Membrane vesicles (MVs) are produced by various Gram positive and Gram negative pathogenic bacteria and play an important role in virulence. In this study, the membrane vesicles (MVs) of L. monocytogenes were isolated from the culture supernatant. Read More

J Food Sci Technol 2019 Mar 7;56(3):1613-1621. Epub 2019 Feb 7.

1Institute for Bioanalysis, Department of Applied Sciences, Coburg University of Applied Sciences and Arts, Friedrich-Streib-Str. 2, 96450 Coburg, Germany.

Nisin is frequently added as food additive to soft cheese to increase food safety against foodborne pathogens like The goal of this study was the extension of the antimicrobial activity of nisin in sour curd cheese (SCC) by self-releasing adsorbed nisin from Neusilin UFL2 over production-based pH shift. First, the antimicrobial activity of nisin adsorbed to Neusilin UFL2 (UFL2-N) and free nisin was investigated in BHI broth at a pH range from 7.5 to 4. Read More

Int J Food Microbiol 2019 Mar 29;299:39-46. Epub 2019 Mar 29.

Department of Animal Science, Iowa State University, Ames, IA, USA; Interdepartmental Microbiology Graduate Program, Iowa State University, Ames, IA, USA. Electronic address:

Listeria monocytogenes is a food-borne pathogen responsible for the disease listeriosis and is commonly isolated from food and food production facilities. Many L. monocytogenes strains contain plasmids, though the contributions of plasmids to survival in food production environments are unknown. Read More

Int J Biol Macromol 2019 Apr 3;132:888-896. Epub 2019 Apr 3.

Laboratory of Microorganisms and Biomolecules (LMB), Centre of Biotechnology of Sfax, Road of Sidi Mansour Km 6, P.O. Box 1177, Sfax 3018, Tunisia. Electronic address:

Stress associated Protein from Lobularia maritima (LmSAP) transcripts showed up-regulation in response to wounding, Methyl-jasmonate acid, Picolinic acid, and ethephon treatments. The antioxidant activities of the purified LmSAP protein were evaluated using various in vitro and in vivo assays. LmSAP has an excellent DPPH scavenging ability and exhibits notable reducing power capacity and led to a decrease in TBARS values. Read More

Res Microbiol 2019 Apr 3. Epub 2019 Apr 3.

INRS-Institut Armand-Frappier, Research Laboratories in Sciences Applied to Food, Canadian Irradiation Centre. 531 boulevard des Prairies. Laval, Quebec, Canada, H7V 1B7. Electronic address:

This paper presents the effects of the composition of different media (i.e., Tryptic soy broth (TSB), Brain heart infusion (BHI), Listeria enrichment broth (LEB), Fraser broth (FB) and University of Vermont medium (UVM)) on the detection of a short peptide fragment PepD specific to the p60 protein (p60) of Listeria. Read More

Appl Environ Microbiol 2019 Apr 5. Epub 2019 Apr 5.

Department of Agricultural Biotechnology, Center for Food and Bioconvergence, Research Institute for Agricultural and Life Sciences, Seoul National University, Seoul 08826, Republic of Korea

In this study, we developed a washing system capable of decontaminating fresh produce by combining the Spindle, which detaches microorganisms on sample surfaces, and a 222-nm krypton-chlorine (KrCl) excilamp (Sp-Ex), and investigated their decontamination effect against O157:H7, Typhimurium, and on apple ( Borkh.) and bell pepper ( L.) surfaces. Read More

Foodborne Pathog Dis 2019 Apr 5. Epub 2019 Apr 5.

1 Department of Experimental Pathology, Immunology and Microbiology, Faculty of Medicine, American University of Beirut, Beirut, Lebanon.

Background: Foodborne diseases are still a major health issue in Lebanon, although some steps have been taken forward in food safety. To this purpose, PulseNet Lebanon, a foodborne diseases tracking network, was established in 2009, through the collaboration between the Ministry of Public Health (MoPH) and the American University of Beirut (AUB).

Materials And Methods: Three papers published regarding the PulseNet project were summarized. Read More

Int J Food Microbiol 2019 Mar 28;299:23-32. Epub 2019 Mar 28.

School of Applied Sciences, Edinburgh Napier University, United Kingdom.

Listeria monocytogenes is sporadically detected on a range of ready to eat fresh produce lines, such as spinach and rocket, and is a threat to public health. However, little is known about the diversity of L. monocytogenes present on fresh produce and their potential pathogenicity. Read More

Microbiol Resour Announc 2019 Mar 14;8(11). Epub 2019 Mar 14.

Departamento de Tecnología de Alimentos, Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA), Madrid, Spain

Here, we present the draft genome sequences of seven strains isolated during three independent studies carried out in three stages of a poultry meat production chain. The genome sequences of these strains obtained from different stages can help to understand the possible transmission of . Read More

Vet World 2019 Jan 17;12(1):90-96. Epub 2019 Jan 17.

Department of Nutrition, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.

Aim: The aim of this study was to evaluate the antibacterial and chemical effect of essential oil (EO), nisin, , and their combination against both and in Iranian white cheese model.

Materials And Methods: Chemical compositions of EO were analyzed by gas chromatography-mass spectrometry. After production of Iranian white cheese, minimum inhibitory concentration (MIC) and minimum bactericidal concentration of EO and nisin and agar spot test of against were evaluated. Read More

Infect Immun 2019 Apr 1. Epub 2019 Apr 1.

Department of Biology, Indiana State University, Terre Haute, Indiana 47809

Cyclic di-adenosine monophosphate (c-di-AMP) is a recently discovered second messenger in bacteria. The cellular level of c-di-AMP in is predicted to be controlled by the synthase DacA and two putative phosphodiesterases, GdpP and Pde2. To investigate the role of c-di-AMP in , we generated null mutants in each of these proteins by gene deletion. Read More

PLoS One 2019 1;14(4):e0214641. Epub 2019 Apr 1.

Department of Food Technology, Prince of Songkla University, Hat Yai, Songkla, Thailand.

Prophages are commonly found in Listeria genomes, potentially enhancing survival or fitness of Listeria spp. Currently, there is still limited information on the distribution of prophages among Listeria isolates of different allelic types and from various sources. In this study, by using mitomycin C induction, prophages were found in 23/144 isolates (16. Read More

Biomed Res Int 2019 26;2019:6937837. Epub 2019 Feb 26.

Laboratoire de Microorganismes et Biomolécules Actives (LR03ES03), Faculté des Sciences de Tunis, Université Tunis El Manar, Campus Universitaire, 2092, Tunis, Tunisia.

Tomatoes and tomato based-foods contain beneficial microorganisms and various organic acids that have important nutritional values for human. The objective of this study was to access the physiochemical properties of fermented tomatoes juices and to evaluate the competitiveness of lactic acid bacteria (LAB) against , , and spp., in artificially contaminated tomato juice. Read More

Front Microbiol 2019 12;10:437. Epub 2019 Mar 12.

Dipartimento di Scienze del Suolo, della Pianta e degli Alimenti, Università di Bari Aldo Moro, Bari, Italy.

The goal of this study was to investigate the adaptation of Scott A cells to treatments with sublethal doses of antimicrobials (ethanol, citral, carvacrol, E-2-hexenal and thyme essential oil). The survival of cells was not affected by the antimicrobials at the concentrations assayed, with the exception of ethanol (1% v/v) and thyme essential oil (100 mg/L), which decreased cell viability from 8.53 ± 0. Read More

J Dairy Sci 2019 Mar 27. Epub 2019 Mar 27.

Department of Health, Animal Science and Food Safety, Università degli Studi di Milano, Via Celoria 10, IT-20133, Milan, Italy.

In the present study, 33 brands of mozzarella cheese (pasteurized cow milk mozzarella obtained by direct acidification through the addition of food-grade citric acid or obtained by natural acidification through the addition of thermophilic starter cultures, mozzarella for pizza mainly obtained by addition of citric acid, and pasteurized buffalo milk mozzarella obtained by adding microbial rennet) were characterized for the factors potentially influencing the growth of Listeria monocytogenes (microbial populations, moisture, pH, and organic acids). Then, the growth potential of L. monocytogenes in mozzarella was investigated by challenge tests performed at different temperatures. Read More