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Front Immunol 2022 9;13:856977. Epub 2022 Jun 9.

Department of Immunology and Microbiology, University of Colorado Anschutz Medical Campus, Aurora, CO, United States.

Naïve T cell activation in secondary lymphoid organs such as lymph nodes (LNs) occurs upon recognition of cognate antigen presented by antigen presenting cells (APCs). T cell activation requires cytoskeleton rearrangement and sustained interactions with APCs. Enabled/vasodilator-stimulated phosphoprotein (Ena/VASP) proteins are a family of cytoskeletal effector proteins responsible for actin polymerization and are frequently found at the leading edge of motile cells. Read More

Front Microbiol 2022 10;13:885502. Epub 2022 Jun 10.

College of Food Science and Biotechnology, Zhejiang Gongshang University, Hangzhou, China.

Various pathogenic and spoilage bacteria frequently coexist in meat processing environments and can form multispecies biofilms, causing significant health and economic issues. Despite the prevalence and coexistence, only less is known about possible interactions between (LM) and spoilers like species, and their community-wide resistance against natural preservatives. This study evaluates the interactions between mono- or dual-species biofilms formed by LM and (PL), as well as the sensitivity of these bacteria in dual-species biofilms to ε-polylysine hydrochloride (ε-PLH) alone or combined with cinnamon essential oil (CEO). Read More

Front Microbiol 2022 8;13:925291. Epub 2022 Jun 8.

College of Food Science and Technology, Yunnan Agricultural University, Kunming, China.

Moringin [4-(α-L-rhamnosyloxy) benzyl isothiocyanate] is an isothiocyanate from seeds. It is the bioactivated form of the glucosinolate precursor glucomoringin with various health benefits. However, few studies have examined the antibacterial activity of moringin. Read More

Front Microbiol 2022 9;13:917964. Epub 2022 Jun 9.

Laboratory of Microbiology and Technology of Marine Products (MICROTEC), Instituto de Investigacións Mariñas (IIM-CSIC), Vigo, Spain.

is considered a foodborne pathogen of serious concern capable of forming multispecies biofilms with other bacterial species, such as spp., adhered onto stainless steel (SS) surfaces. In an attempt to link the biofilms' morphology and resistance to biocides, dual-species biofilms of , in co-culture with either , , or , were assayed to ascertain their morphological characteristics and resistance toward benzalkonium chloride (BAC) and neutral electrolyzed water (NEW). Read More

Front Microbiol 2022 9;13:894334. Epub 2022 Jun 9.

Department of Preventive Medicine, University of the Basque Country (UPV/EHU), San Sebastián, Spain.

Background: Listeriosis continues to be one of the most important notifiable foodborne diseases. Nonetheless, in Spain, there are few data on the molecular epidemiology of infections in recent years.

Aim: To describe clinical features and the molecular epidemiology of human listeriosis over an 11-year period (2010-2020) in Gipuzkoa, Northern Spain. Read More

Infection 2022 Jun 26. Epub 2022 Jun 26.

Unidad de Gestión Clínica Interniveles de Prevención, Promoción y Vigilancia de La Salud, Hospital Universitario Reina Sofía, Córdoba, Spain.

Background: Listeriosis presents high rates of mortality but prognostic factors for early prevention are not well established. The aim of this study was to analyse factors associated with in-hospital and early mortality of adults after recovery from severe infection caused by Listeria monocytogenes.

Methods: All cases of listeriosis notified in the province of Granada from January 2005 to December 2021, including 9 centres, were included. Read More

J Appl Microbiol 2022 Jun 25. Epub 2022 Jun 25.

School of Food Science and Engineering, South China University of Technology, Guangzhou, China.

Aims: The aim of the current study was to investigate the effect of plasma-mediated oxidative stress on the post-treatment viability of Listeria monocytogenes at the physiological and molecular levels.

Methods And Results: 10 CFU/mL L. monocytogenes in 10 mL phosphate-buffered saline (PBS) were treated with atmospheric nonthermal plasma for 0, 30, 60, 90 and 120 s, respectively. Read More

Int J Food Microbiol 2022 Jun 10;378:109784. Epub 2022 Jun 10.

Department of Veterinary Sciences, University of Turin, Largo Braccini 2, Grugliasco, 10095 Turin, Italy.

Listeria monocytogenes is considered a major challenge for the food industry as it can persist for long periods in food processing plants by forming biofilms. The aims of this study were: i) to assess the biofilm producing ability of 57 Listeria monocytogenes isolates previously subjected to whole-genome sequencing (WGS); ii) to compare the levels of biofilm formation with the presence or absence of biofilm associated genes. To determine the presence or absence of a known set of biofilm associated genes, a comparative genomic analysis was performed on each strain. Read More

J Food Prot 2022 Jun 24. Epub 2022 Jun 24.

Tokyo University of Marine Science and Technology, Department of Food Science and Technology 4-5-7, Konan, Minato-ku JAPAN Tokyo 1088477.

Although essential oils (EOs) exhibit antimicrobial properties, its application is limited owing to their strong volatility and poor water solubility. Emulsification is a valid strategy for improving chemical stability. In this study, we prepared a mustard essential oil (MO) emulsion with egg yolk lecithin and evaluated its antimicrobial activity against Listeria monocytogenes in vitro and in cheese curd. Read More

Klin Lab Diagn 2022 Jun;67(6):362-368

FSBI «Academy of Postgraduate Education within FNKC FMBA of Russia».

Results from research on isolation, identification, and study of biological properties of L. monocytogenes clinical isolates and Listeria spp test strains are presented. Peculiarities of modern research methods for indicating and identifying pathogenic listeria to improve the quality of laboratory studies of clinical material are studied. Read More

Afr J Lab Med 2022 23;11(1):1482. Epub 2022 May 23.

Infection Control Services Laboratory, National Health Laboratory Services, Johannesburg, South Africa.

Background: The 2017-2018 listeriosis outbreak in South Africa warranted testing for in food products and processing environments. Diagnostic tests are needed to accurately differentiate from other species.

Objective: The study assessed the performance of the commonly used tests in our setting to accurately identify . Read More

Pathogens 2022 Jun 16;11(6). Epub 2022 Jun 16.

Department of Nutrition and Food Science, University of Maryland, College Park, MD 20742, USA.

Despite its low morbidity, listeriosis has a high mortality rate due to the severity of its clinical manifestations. The source of human listeriosis is often unclear. In this study, we investigate the ability of machine learning to predict the food source from which clinical isolates originated. Read More

Pathogens 2022 Jun 8;11(6). Epub 2022 Jun 8.

Food Microbiology Unit, Istituto Zooprofilattico Sperimentale del Lazio e della Toscana "M. Aleandri", 00178 Rome, Italy.

Listeriosis is currently the fifth most common foodborne disease in Europe. Most cases are sporadic; however, outbreaks have also been reported. Compared to other foodborne infections, listeriosis has a modest incidence but can cause life-threatening complications, especially in elderly or immunocompromised people and pregnant women. Read More

Nanomaterials (Basel) 2022 Jun 7;12(12). Epub 2022 Jun 7.

Centro Universitario de los Altos, Laboratorio de Investigación en Nanomateriales, Agua y Energía, Departamento de Ingeniería, Universidad de Guadalajara, Av. Rafael Casillas Aceves 1200, Tepatitlán de Morelos 47600, Mexico.

TiO, ZnO, and their combination (TiO-ZnO) at different molar ratios and pH values (Ti-Zn A and B 3:1, 1:1, and 1:3) via the sol-gel method were characterized by SEM, XRD, UV-Vis, and FT-IR. Moreover, antibacterial tests of the nanoparticles were conducted against (), (), (), and (). The indirect bandgap of the Ti-Zn binary oxide synthesized in the basic process at molar ratios of 3:1, 1:1, and 1:3 exhibited a higher eV (3. Read More

Molecules 2022 Jun 9;27(12). Epub 2022 Jun 9.

Laboratory of Materials Molecules and Applications (LMMA), Preparatory Institute of Scientific and Technical Studies (IPEST), University of Carthage, Tunis 2070, Tunisia.

This study aimed to evaluate the effects of thyme ( L.), rosemary ( L.), and ammoides ( L. Read More

Foods 2022 Jun 15;11(12). Epub 2022 Jun 15.

School of Nutrition and Food Sciences, Louisiana State University Agricultural Center, Baton Rouge, LA 70803, USA.

is a serious public health hazard responsible for the foodborne illness listeriosis. is ubiquitous in nature and can become established in food production facilities, resulting in the contamination of a variety of food products, especially ready-to-eat foods. Effective and risk-based environmental monitoring programs and control strategies are essential to eliminate in food production environments. Read More

Foods 2022 Jun 8;11(12). Epub 2022 Jun 8.

Institute of Agri-food Processing and Nutrition, Beijing Key Laboratory of Agricultural Products of Fruits and Vegetables Preservation and Processing, Key Laboratory of Vegetable Postharvest Processing, Ministry of Agriculture and Rural Affairs, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, China.

Microbial community distribution in vegetables can affect their quality. This study analyzed the distribution of the microbial community at various stages during processing and storage with the microbial diversity analysis, and evaluated the correlation between the dominant bacteria and sensory quality of lettuce using correspondence analysis with multiple regression analysis. Results showed that the process of washing, cutting, then disinfection and dewatering could change the community distribution and dominant bacteria in lettuce, and maintain better texture, morphology, aroma, color qualities of lettuce. Read More

Biology (Basel) 2022 Jun 6;11(6). Epub 2022 Jun 6.

Veterinary Faculty, University of Thessaly, 43100 Karditsa, Greece.

A cross-sectional study was performed in 325 sheep and 119 goat dairy farms in Greece. Samples of bulk-tank milk were examined by standard microbiological techniques for spp. was isolated from one (0. Read More

Antibiotics (Basel) 2022 May 27;11(6). Epub 2022 May 27.

Institute of Food Sciences, CNR-ISA, Via Roma, 64, 83100 Avellino, Italy.

This work aimed to evaluate the chemical composition of the essential oils (EOs) of two cultivars of and their antibiofilm activity against the food pathogens , , , and . The crystal violet assay ascertained the susceptibility of the bacterial biofilms, while the MTT assay let to evaluations of the metabolic changes occurring in the bacterial cells within biofilms. Their chemical composition indicated some sulfuric compounds (i. Read More

NPJ Vaccines 2022 Jun 23;7(1):61. Epub 2022 Jun 23.

Department of Pharmacology, Physiology, & Cancer Biology, Sidney Kimmel Medical College, Thomas Jefferson University, Philadelphia, PA, 19107, USA.

Strategies to augment immunity to self/neoantigens expressed by cancers are urgently needed to expand the proportion of patients benefiting from immunotherapy, particularly for GI cancers where only a fraction of patients respond to immunotherapies. However, current vaccine strategies are limited by poor immunogenicity, pre-existing vector-specific immunity, and vaccine-induced vector-specific immunity. Here, we examined a prime-boost strategy using a chimeric adenoviral vector (Ad5. Read More

Mol Cell 2022 Jun 17. Epub 2022 Jun 17.

Department of Biochemistry and Molecular Biology, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China; Department of Immunology & National Key Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences (CAMS) & Peking Union Medical College, Beijing, China; State Key Laboratory of Experimental Hematology, National Clinical Research Center for Blood Diseases, Haihe Laboratory of Cell Ecosystem, Institute of Hematology & Blood Diseases Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Tianjin 300020, China. Electronic address:

Glycolysis facilitates the rapid recall response of CD8 memory T (Tm) cells. However, it remains unclear whether Tm cells uptake exogenous glucose or mobilize endogenous sugar to fuel glycolysis. Here, we show that intracellular glycogen rather than extracellular glucose acts as the major carbon source for the early recall response. Read More

Microbiol Spectr 2022 Jun 23:e0071022. Epub 2022 Jun 23.

Department of Food Science and Nutrition, Zhejiang Universitygrid.13402.34, Hangzhou, China.

Listeria monocytogenes (Lm) is a foodborne pathogen that can cause severe human illness. Standard control measures for restricting bacterial growth, such as refrigeration, are often inadequate as Lm grows well at low temperatures. To identify genes involved in growth at low temperatures, a powerful functional genomics method Tn-seq was performed in this study. Read More

JAC Antimicrob Resist 2022 Jun 17;4(3):dlac061. Epub 2022 Jun 17.

University of Tartu, Institute of Technology, 50411 Tartu, Estonia.

Background: Listeriosis is a food-borne disease caused by the Gram-positive Bacillota (Firmicute) bacterium . Clinical isolates are often resistant to clinically used lincosamide clindamycin, thus excluding clindamycin as a viable treatment option.

Objectives: We have established newly developed lincosamide iboxamycin as a potential novel antilisterial agent. Read More

J Med Microbiol 2022 Jun;71(6)

Clinical Unit of Infectious Diseases, Microbiology and Preventive Medicine, Infectious Diseases. Virgen del Rocío University Hospital, Institute of Biomedicine of Seville (IBIS), University of Seville/CSIC/University Hospital Virgen del Rocío, Seville, Spain.

Microorganisms produce a wide variety of volatile organic compounds (VOCs) as products of their metabolism and some of them can be specific VOCs linked to the microorganism's identity, which have proved to be helpful for the diagnosis of infection via odour fingerprinting. The aim of this study was to determine the VOCs produced and consumed to characterize the volatile metabolism of seven isolates of different clonal complexes (CCs) of . For this purpose, dichloromethane extracts from the thioglycolate broth medium were analysed by gas chromatography coupled to mass spectrometry (GC/MS). Read More

J Food Prot 2022 Jun 20. Epub 2022 Jun 20.

Cornell University Department of Food Science 347 Stocking Hall UNITED STATES Ithaca New York 14853 6072275903.

While public health risk assessments for Listeria monocytogenes (Lm) have been published for different foods, firm-level decision making on interventions targeting Lm involves considerations of both public health and enterprise risks. Smoked seafood is a ready-to-eat product with a high incidence of Lm contamination and associated with several recalls. We thus used cold-smoked salmon as a model product to develop a decision support tool (the Regulatory and Recall Risk [3R] Model) to estimate (i) baseline regulatory and recall (RR) risks (i. Read More

BMC Microbiol 2022 Jun 18;22(1):160. Epub 2022 Jun 18.

Department of Disease Control, School of Veterinary Medicine, University of Zambia, Lusaka, 10101, Zambia.

Background: The food industry is increasingly becoming more scrutinized, given the frequency and intensity with which zoonotic diseases are being reported. Pathogen tracking has become more applicable with regards food safety. It is in this regard that the present study was formulated to track Listeria species. Read More

Fish Shellfish Immunol 2022 Jun 15;127:119-128. Epub 2022 Jun 15.

State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, Ningbo University, Ningbo, 315211, China; Laboratory of Biochemistry and Molecular Biology, School of Marine Sciences, Ningbo University, Ningbo, 315211, China; Key Laboratory of Aquacultural Biotechnology Ministry of Education, Ningbo University, Ningbo, 315211, China. Electronic address:

Peptidoglycan recognition proteins (PGRPs) belong to a member of pattern-recognition receptors (PRRs), which proposed as antibacterial protein. The present study investigated the antibacterial effect of BpPGRP5 in great blue-spotted mudskipper (Boleophthalmus pectinirostris). BpPGRP5 transcript was detected in all tested tissues with the highest expression level in spleen, and its expression was significantly upregulated in spleen, intestine, and kidney following Aeromonas veronii infection. Read More

Lett Appl Microbiol 2022 Jun 18. Epub 2022 Jun 18.

Department of Biotechnology, Faculty of Advanced Sciences and Technology, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran.

This study was done to find exopolysaccharide(EPS)-producing lactic acid bacteria (LAB) against foodborne pathogens. Isolated LAB were screened to find the ones with the ability to produce antibacterial EPS against foodborne pathogens. Among tested EPSs, EPS of AS20(1) isolate showed inhibitory effects on the growth of Listeria monocytogenes (MIC= 0. Read More

Sci Immunol 2022 Jun 17;7(72):eabf1861. Epub 2022 Jun 17.

Department of Oncology, Microbiology and Immunology, University of Fribourg, 1700 Fribourg, Switzerland.

The gastrointestinal (GI) tract constitutes an essential barrier against ingested microbes, including potential pathogens. Although immune reactions are well studied in the lower GI tract, it remains unclear how adaptive immune responses are initiated during microbial challenge of the oral mucosa (OM), the primary site of microbial encounter in the upper GI tract. Here, we identify mandibular lymph nodes (mandLNs) as sentinel lymphoid organs that intercept ingested (Lm). Read More

Sci Immunol 2022 Jun 17;7(72):eabp8632. Epub 2022 Jun 17.

Oral Immunity and Infection Section, NIDCR, NIH, Bethesda, MD, USA.

In this issue of , Barreto de Albuquerque track immune responsiveness to the foodborne pathogen during oral infection. Their findings extend the notion of compartmentalized immunity within the gastrointestinal tract to the oral cavity and provide previously unkown insights into regional specialization of oral immunity. Read More