31,288 results match your criteria Light-Chain Deposition Disease


Propofol partially attenuates complete freund's adjuvant-induced neuroinflammation through inhibition of the ERK1/2/NF-κB pathway.

J Cell Biochem 2018 Dec 9. Epub 2018 Dec 9.

Department of Anesthesiology, Quzhou Hospital of Zhejiang University School of Medicine, Quzhou, China.

Peripheral inflammation in male C57BL/6 mice was induced by intraplantar injection of 20 μL complete freund's adjuvant (CFA) in the left hind paw. Mice were randomly divided into three groups: Sham, CFA, and propofol+CFA. Mechanical allodynia was assessed by von Frey analysis, and heat hyperalgesia was detected by exposure of the plantar surface to a beam of radiant heat. Read More

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December 2018

Plumbagin induces autophagy and apoptosis of SMMC-7721 cells in vitro and in vivo.

J Cell Biochem 2018 Dec 9. Epub 2018 Dec 9.

Department of Physiology, Guangxi University of Chinese Medicine, Nanning, Guangxi, China.

Plumbagin (PL), an active naphthoquinone compound, has been demonstrated to be a potential anticancer agent. However, the underlying anticancer mechanism is not fully understood. In this study, the human hepatocellular carcinoma (HCC) SMMC-7721 cell line was studied in an in vitro model. Read More

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December 2018

Effect of miR-21/TLR4/NF-κB pathway on myocardial apoptosis in rats with myocardial ischemia-reperfusion.

Eur Rev Med Pharmacol Sci 2018 Nov;22(22):7928-7937

Department of Cardiology, The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University, Wenzhou, China.

Objective: The aim of the study was to investigate the influences of micro ribonucleic acid (miR)-21 and downstream Toll-like receptor 4 (TLR4)/nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) pathway on myocardial apoptosis induced by myocardial ischemia-reperfusion (I/R) injury in rats.

Materials And Methods: Recombinant adeno-associated virus rAAV9-ZsGreen-pre-miR-21 and blank control virus were constructed. A total of 48 Sprague-Dawley (SD) rats were randomly divided into S1 group (open chest only), S2 group (transfection with blank virus + open chest), I/R1 group (transfection with blank virus + 6 d of myocardial I/R), and I/R2 group (transfection with miR-21 + 6 d of myocardial I/R). Read More

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November 2018

Inhibition of autophagy enhances adenosine‑induced apoptosis in human hepatoblastoma HepG2 cells.

Oncol Rep 2018 Nov 30. Epub 2018 Nov 30.

Department of Gastroenterology, The Second Affiliated Hospital, Shantou University Medical College, Shantou, Guangdong 515041, P.R. China.

In cancer research, autophagy acts as a double‑edged sword: it increases cell viability or induces cell apoptosis depending upon the cell context and functional status. Recent studies have shown that adenosine (Ado) has cytotoxic effects in many tumors. However, the role of autophagy in Ado‑induced apoptosis is still poorly understood. Read More

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November 2018

α‑lipoic acid protects against carbon tetrachloride‑induced liver cirrhosis through the suppression of the TGF‑β/Smad3 pathway and autophagy.

Mol Med Rep 2018 Dec 4. Epub 2018 Dec 4.

Spleen, Stomach and Hepatobiliary Department, The First Affiliated Hospital, Henan University of Chinese Medicine, Zhengzhou, Henan 450004, P.R. China.

α‑lipoic acid (ALA) is a naturally occurring antioxidant with protective effects against various hepatic injuries. The aim of the present study was to investigate the mechanisms by which ALA protects the liver from carbon tetrachloride (CCl4)‑induced liver cirrhosis. The widely used liver cirrhosis rat model was established via an intraperitoneal injection of 2 mg/kg 50% CCl4, three times/week for 8 weeks. Read More

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December 2018

Panax Notoginseng Saponins Attenuate Myocardial Ischemia Reperfusion Injury via the HIF-1α/BNIP3 Pathway of Autophagy.

J Cardiovasc Pharmacol 2018 Nov 30. Epub 2018 Nov 30.

Department of Cardiovascular medicine, the Affiliated Hospital of Shaoxing University, Shaoxing 312000, PR China.

Background And Objective: Panax Notoginseng Saponins (PNS) is a formula of Chinese Medicine commonly used for treating ischemia myocardial in China. However, its mechanism of action is yet unclear. This study investigated the effect and the mechanism of PNS on myocardial ischemia-reperfusion injury via the hypoxia-inducible factor 1α(HIF-1α)/bcl-2/adenovirus E1B19kDa-interacting protein3(BNIP3) pathway of autophagy. Read More

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November 2018

Neuronal autophagy aggravates microglial inflammatory injury by downregulating CX3CL1/fractalkine after ischemic stroke.

Neural Regen Res 2019 Feb;14(2):280-288

Department of Basic Medicine, Medical School, Kunming University of Science and Technology, Kunming, Yunnan Province, China.

Ischemic stroke often induces excessive neuronal autophagy, resulting in brain damage; meanwhile, inflammatory responses stimulated by ischemia exacerbate neural injury. However, interactions between neuronal autophagy and microglial inflammation following ischemic stroke are poorly understood. CX3CL1/fractalkine, a membrane-bound chemokine expressed on neurons, can suppress microglial inflammation by binding to its receptor CX3CR1 on microglia. Read More

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February 2019

Drak/STK17A drives neoplastic glial proliferation through modulation of MRLC signaling.

Cancer Res 2018 Dec 10. Epub 2018 Dec 10.

Department of Pharmacology, Emory University

Glioblastoma (GBM) and lower grade gliomas (LGG) are the most common primary malignant brain tumors and are resistant to current therapies. Genomic analyses reveal that signature genetic lesions in GBM and LGG include copy gain and amplification of chromosome 7, amplification, mutation, and overexpression of receptor tyrosine kinases (RTK) such as EGFR, and activating mutations in components of the PI-3 kinase (PI3K) pathway. In Drosophila melanogaster, constitutive co-activation of RTK and PI3K signaling in glial progenitor cells recapitulates key features of human gliomas. Read More

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December 2018

Varying magnesium concentration elicits changes in inflammatory response in human umbilical vein endothelial cells (HUVECs).

Magnes Res 2018 Dec 10. Epub 2018 Dec 10.

Division of Nutritional Sciences, School of Biosciences, The University of Nottingham, United Kingdom.

The aims of this study were to determine whether low concentrations of magnesium in vitro exacerbated the human umbilical vein endothelial cell (HUVEC) response to inflammatory challenge, and whether expression of the nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) through the toll-like receptor 4 (TLR4) played a role in this process. HUVECs were incubated with different concentrations of Mg (low- 0.1mM, control- 1mM, high- 5mM) for 72 h before being stimulated with bacterial lipopolysaccharide (LPS) for 4 h. Read More

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December 2018

Review: Fluid biomarkers in the human prion diseases.

Mol Cell Neurosci 2018 Dec 4. Epub 2018 Dec 4.

MRC Prion Unit at University College London (UCL), UCL Institute of Prion Diseases, UCL, London W1W 7FF, United Kingdom of Great Britain and Northern Ireland. Electronic address:

The human prion diseases are a diverse set of often rapidly progressive neurodegenerative conditions associated with abnormal forms of the prion protein. We review work to establish diagnostic biomarkers and assays that might fill other important roles, particularly those that could assist the planning and interpretation of clinical trials. The field now benefits from highly sensitive and specific diagnostic biomarkers using cerebrospinal fluid: detecting by-products of rapid neurodegeneration or specific functional properties of abnormal prion protein, with the second generation real time quaking induced conversion (RT-QuIC) assay being particularly promising. Read More

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December 2018

Current understanding of conventional and novel Co-Expression patterns of mammalian sarcomeric myosin heavy chains and light chains.

Authors:
Peter J Reiser

Arch Biochem Biophys 2018 Dec 5. Epub 2018 Dec 5.

Division of Biosciences, College of Dentistry, The Ohio State University, 305 West 12th Avenue, Columbus, OH, 43210, USA. Electronic address:

A central tenet of muscle physiology that has accrued from several decades of intense investigations is that myosin, and the vast set of isoforms that constitute its six subunits, is a major regulator of contractile properties of smooth, cardiac and skeletal muscle. Two frequent questions are (1) how many myosin heavy chain (MyHC) isoforms and myosin light chain (MLC) isoforms are expressed in mammalian striated muscles and (2) which isoforms of MyHC and MLC are expressed, at the protein level, with each other - that is, what patterns of co-expression exist in single striated muscle fibers? The answer to the former question is straightforward: eleven MyHC isoforms and nine MLC isoforms, are expressed in a developmentally-regulated and muscle-specific manner. The answer to the latter question, on the other hand, is not clear-cut. Read More

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December 2018

38Missing-in-metastasis protein promotes internalization of magnetic nanoparticles via association with clathrin light chain and Rab7.

Biochim Biophys Acta Gen Subj 2018 Dec 6. Epub 2018 Dec 6.

State Key Laboratory of Bioelectronics, Jiangsu Key Laboratory for Biomaterials and Devices, School of Biological Science and Medical Engineering, Southeast University, Nanjing 210096, PR China; Collaborative Innovation Center of Suzhou Nano Science and Technology, Suzhou 215123, PR China. Electronic address:

Background: Magnetic nanoparticles (MNPs) have been widely used in biomedical applications. Proper control of the duration of MNPs in circulation promises to improve further their applications, in particularly drug delivery. It is known that the uptake of tissue-associated MNPs is mainly carried out by macrophages. Read More

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December 2018

Texture analysis of cardiovascular magnetic resonance cine images differentiates aetiologies of left ventricular hypertrophy.

Clin Radiol 2018 Oct 24. Epub 2018 Oct 24.

Bart's Heart Centre, London, UK; Institute of Cardiovascular Science, University College London, UK.

Aim: To investigate whether unenhanced cardiovascular magnetic resonance (CMR) balanced steady state free precession (bSSFP) cine images could be analysed using textural analysis (TA) software to differentiate different aetiologies of disease causing increased myocardial wall thickness (left ventricular hypertrophy [LVH]) and indicate the severity of myocardial tissue abnormality.

Materials And Methods: A mid short axis unenhanced cine frame of 216 patients comprising 50 cases of hypertrophic cardiomyopathy (HCM; predominantly Left ventricular outflow tract obstruction [LVOTO] subtype), 52 cases of cardiac amyloid (CA; predominantly AL: light chain subtype), 68 cases of aortic stenosis (AS), 15 hypertensive patients with LVH (HTN+LVH), and 31 healthy volunteers (HV) underwent TA of the CMR cine images (CMRTA) using TexRAD (TexRAD Ltd, Cambridge, UK). Among the HV, 16/31 were scanned twice to form a test-retest reproducibility cohort. Read More

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October 2018

Two Double-hit Lymphomas Cases: A Molecular Cytogenetic Approach.

J Assoc Genet Technol 2018 ;44(4):141-145

Allina Health, Minneapolis, MN.

Objectives: Double-hit lymphomas represent 5% of cases of diffuse large B-cell lymphomas (DLBCL). They are currently recognized as highgrade B-cell lymphomas (HGBCL) with rearrangements of MYC and BCL2 and/or BCL6 by the 2016 WHO classification. One of these rearrangements is the translocation of the BCL2 gene (18q21. Read More

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January 2018
2 Reads

Activation of GPR120 promotes the metastasis of breast cancer through the PI3K/Akt/NF-κB signaling pathway.

Anticancer Drugs 2018 Dec 4. Epub 2018 Dec 4.

Department of Laboratory Medicine, The First Affiliated Hospital with Nanjing Medical University, Nanjing, Jiangsu Province 210029, China.

G-protein-coupled receptor 120 (GPR120) plays an important role in regulating lipid and glucose metabolism as a long-chain unsaturated fatty acid receptor. However, it has been widely accepted that omega-3 polyunsaturated fatty acids are not dependent on the activation of GPR120 to exert anti-tumor activity. Therefore, the role of GPR120 in tumor development has not yet been elucidated. Read More

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December 2018
3 Reads

The Combination of Omega-3 Stearidonic Acid and Docetaxel Enhances Cell Death over Docetaxel Alone in Human Prostate Cancer Cells.

J Cancer 2018 11;9(23):4536-4546. Epub 2018 Nov 11.

Department of Anatomy, Physiology, and Pharmacology, College of Veterinary Medicine, Auburn University, Auburn, AL.

: Docetaxel (DOC), or Taxotere, is an anthracycline antibiotic used to treat multiple types of cancer. It is a first-line chemotherapy treatment for patients with metastasized, hormone-resistant prostate cancer (PCa) or for patients with high-risk, localized PCa that could benefit from early chemotherapy treatment. Previously, we showed that stearidonic acid (SDA), an omega-3 fatty acid, enhances the cytotoxicity of doxorubicin (DOX) in human PCa cells. Read More

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November 2018
1 Read

Promotion of Cell Proliferation through Inhibition of Cell Autophagy Signalling Pathway by Rab3IP is Restrained by MicroRNA-532-3p in Gastric Cancer.

J Cancer 2018 22;9(23):4363-4373. Epub 2018 Oct 22.

Department of General Surgery, Nanfang Hospital, Southern Medical University, Guangdong Provincial Engineering Technology Research Center of Minimally Invasive Surgery, Guangzhou 510515, China.

RAB3A-interacting protein (Rab3IP) is known to be involved in cancer; however, its function during the proliferation of gastric cancer (GC) cells remains unknown. Therefore, this study aimed to explore the potential function of Rab3IP in GC. The expression of Rab3IP and its clinical pathology value were determined by quantitative real-time PCR and immunohistochemistry. Read More

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October 2018

Prognostic value of TIGAR and LC3B protein expression in nasopharyngeal carcinoma.

Cancer Manag Res 2018 12;10:5605-5616. Epub 2018 Nov 12.

Department of Oncology, The Affiliated Hospital of Southwest Medical University, Luzhou 646000, People's Republic of China,

Purpose: Autophagy, the process responsible for degrading cytoplasmic organelles to sustain cellular metabolism, has been associated with cancer initiation and progression. As TP53-induced glycolysis and apoptosis regulator () is among the important genes that can regulate autophagy, we aimed to investigate the correlation between the expression levels of TIGAR and the autophagy-related protein microtubule-associated protein 1 light chain 3 (), as well as their association with clinical outcomes, in nasopharyngeal carcinoma (NPC) patients.

Methods: We detected the expressions of TIGAR and LC3B in 182 NPC tissue samples via immunohistochemical staining. Read More

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November 2018

Metformin ameliorated methotrexate-induced hepatorenal toxicity in rats in addition to its antitumor activity: two birds with one stone.

J Inflamm Res 2018 8;11:421-429. Epub 2018 Nov 8.

Department of Tropical, Faculty of Medicine, Tanta University, Tanta, Egypt,

Methotrexate (MTX) is a drug used in treatment of various malignancies. Unfortunately, it leads to life-threatening complications including hepatorenal toxicity. Previous studies revealed the protective effects of metformin (MET) on hepatorenal toxicity in other models in addition to its anticancer effects. Read More

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November 2018
1 Read

Cell-based immunotherapy approaches for multiple myeloma.

Br J Cancer 2018 Dec 6. Epub 2018 Dec 6.

Department of Hematology, Oncology and Rheumatology, Heidelberg University, Heidelberg, Germany.

Despite the arrival of novel therapies, multiple myeloma (MM) remains incurable and new treatment options are needed. Chimeric antigen receptor (CAR) T cells are genetically modified T cells that express a CAR directed against specific tumour antigens. CAR T cells are able to kill target tumour cells and may result in long-lasting immune responses in vivo. Read More

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December 2018

17 β-Estradiol Oxidative Stress Attenuation and Autophagy-Induced Dopaminergic Neuroprotection.

Cell J 2019 Apr 18;21(1):1-6. Epub 2018 Nov 18.

Department of Clinical Biochemistry, Faculty of Medicine, Ilam University of Medical Sciences, Ilam, Iran.

Objective: Degeneration of dopaminergic neurons in the substantia nigra of the brain stem is the main pathological aspect of Parkinson's disease (PD). 17 β-estradiol (E2) has neuroprotective effects on substantia nigra, however, the underlined mechanism is not well-known. In this study, we evaluated the neuroprotective effects of E2 in the ovariectomized 6-hydroxydopamine- (6-OHDA) rat model of PD. Read More

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Cr(VI)-Induced Autophagy Protects L-02 Hepatocytes from Apoptosis Through the ROS-AKT-mTOR Pathway.

Cell Physiol Biochem 2018 30;51(4):1863-1878. Epub 2018 Nov 30.

Department of Health Toxicology, Xiangya School of Public Health, Central South University, Changsha,

Background/aims: Hexavalent chromium [Cr(VI)] pollution has become a global concern for both ecosystems and human health. Our previous study revealed Cr(VI) could induce both apoptosis and autophagy in L-02 hepatocytes. Here, we sought to explore the underlying mechanism of Cr(VI)-induced autophagy and its exact role in cell death. Read More

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November 2018

LC3A, LC3B and Beclin-1 Expression in Gastric Cancer.

Anticancer Res 2018 Dec;38(12):6827-6833

Department of Pathology, Democritus University of Thrace and University General Hospital of Alexandroupolis, Alexandroupolis, Greece.

Background: The current study examined the key proteins involved in autophagosome formation and their prognostic role in gastric cancer.

Materials And Methods: Paraffin-embedded tissues from 121 consecutive patients treated with surgery for gastric cancer were analyzed immunohistochemically for the expression of autophagic proteins microtubule-associated proteins 1A/1B light chain 3A and 3B (LC3A, LC3B) and beclin-1 (encoded by BECN1 gene). Assessment of proliferative index using E3 ubiquitin-protein ligase (MIB1) and correlations with histopathological parameters and overall survival were performed. Read More

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December 2018

Approach to the treatment of the older, unfit patient with myeloma from diagnosis to relapse: perspectives of a US hematologist and a geriatric hematologist.

Hematology Am Soc Hematol Educ Program 2018 Nov;2018(1):88-96

Jerome Lipper Multiple Myeloma Center, LeBow Institute for Myeloma Therapeutics, Medical Oncology, Dana-Farber Cancer Institute, Harvard Medical School, Boston, MA.

Mrs. A. is a 73-year-old woman who has developed increasing fatigue and lower back pain over the past year. Read More

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November 2018

Treatment approach for the older, unfit patient with myeloma from diagnosis to relapse: perspectives of a European hematologist.

Hematology Am Soc Hematol Educ Program 2018 Nov;2018(1):83-87

Jerome Lipper Multiple Myeloma Center, LeBow Institute for Myeloma Therapeutics, Dana-Farber Cancer Institute, Harvard Medical School, Boston, MA.

Mrs. A. is a 73-year-old woman who has developed increasing fatigue and lower back pain over the past year. Read More

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November 2018

The deregulation of STIM1 and store operative calcium entry (SOCE) impaired aortic smooth muscle cells contractility in aortic medial degeneration.

Biosci Rep 2018 Nov 30. Epub 2018 Nov 30.

Cardiovascular surgery, Renmin hospital of Wuhan university, No. 99 ZhangZhiDong Street Wuchang District, Wuhan, Hubei Province, China, Wuhan, China

Background:  Micro-array analysis of clinical aortic samples suggested a potential role for STIM1 in the modulation of aortic medial degeneration (AMD), despite the uncertainty about STIM1 in normal aortic smooth muscle cells (ASMCs). Here, we aimed to explore changes in STIM1 expression in AMD, and the possible mechanisms. Methods: An AMD model was established using auto-delivery of angiotensin II into ApoE-/- mice. Read More

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November 2018
2.637 Impact Factor

[Bone seeking tracers' scintigraphy for the diagnosis of transthyretin cardiac amyloidosis].

Medicina (B Aires) 2018 ;78(6):395-398

Sección Medicina Nuclear, Servicio de Diagnóstico por Imágenes, Instituto Cardiovascular de Buenos Aires (ICBA), Buenos Aires, Argentina.

Transthyretin cardiac amyloidosis (ATTR) is a restrictive cardiomyopathy that leads to heart failure in considerable number of patients. Early diagnosis allows specific treatment options. However, ATTR diagnosis is complex and requires invasive procedures. Read More

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January 2018

The molecular mechanism of induction of unfolded protein response by Chlamydia.

Biochem Biophys Res Commun 2018 Nov 28. Epub 2018 Nov 28.

Centers for Disease Control and Prevention (CDC), Atlanta, GA, USA; Morehouse School of Medicine, Atlanta, GA, USA. Electronic address:

The unfolded protein response (UPR) contributes to chlamydial pathogenesis, as a source of lipids and ATP during replication, and for establishing the initial anti-apoptotic state of host cell that ensures successful inclusion development. The molecular mechanism(s) of UPR induction by Chlamydia is unknown. Chlamydia use type III secretion system (T3SS) effector proteins (e. Read More

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November 2018

Nicotinamide Promotes Cell Survival and Differentiation as Kinase Inhibitor in Human Pluripotent Stem Cells.

Stem Cell Reports 2018 Nov 15. Epub 2018 Nov 15.

Centre of Reproduction, Development & Aging, Faculty of Health Sciences, University of Macau, Taipa, Macau, China; Institute of Translational Medicine, Faculty of Health Sciences, University of Macau, Taipa, Macau, China. Electronic address:

Nicotinamide, the amide form of vitamin B3, is widely used in disease treatments and stem cell applications. However, nicotinamide's impact often cannot be attributed to its nutritional functions. In a vitamin screen, we find that nicotinamide promotes cell survival and differentiation in human pluripotent stem cells. Read More

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November 2018

Lower number of 5-hydroxymethylcytosine-expressing cells in plasma cell myeloma than in reactive plasma cell hyperplasia: a useful immunohistochemical approach for identification of neoplastic plasma cells.

Pathology 2018 Nov 28. Epub 2018 Nov 28.

Department of Pathology, Shinshu University School of Medicine, Matsumoto, Japan. Electronic address:

Plasma cell myeloma (PCM) is a haematological malignancy defined by aberrant proliferation of plasma cells in the bone marrow. For the diagnosis of PCM, immunostaining of light chains or surface marker analyses by flow cytometry is needed but sometimes difficult. Aberrant methylation at the carbon-5 position of cytosine results in 5-methylcytosine (5-mC), which is a well-known epigenetic hallmark of cancer in mammals. Read More

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November 2018

Proresolving Lipid Mediators Resolvin D1 and Protectin D1 Isomer Attenuate Neointimal Hyperplasia in the Rat Carotid Artery Balloon Injury Model.

J Surg Res 2019 Jan 18;233:104-110. Epub 2018 Aug 18.

Division of Vascular Surgery, Department of Surgery, Graduate School of Medicine, The University of Tokyo, Tokyo, Japan.

Background: Specialized proresolving mediators from ω-3 polyunsaturated fatty acid may control resolution of inflammation. We evaluated the influence of two specialized proresolving mediators, resolvin D1 (RvD1) and protectin D1 isomer (PD1 iso) on neointimal hyperplasia after balloon injury.

Materials And Methods: Sprague Dawley male rats at 12-14 wk of age were injured as a model of balloon angioplasty. Read More

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January 2019

Cerebrospinal fluid biomarkers of neurofibrillary tangles and synaptic dysfunction are associated with longitudinal decline in white matter connectivity: A multi-resolution graph analysis.

Neuroimage Clin 2018 Oct 23. Epub 2018 Oct 23.

Department of Biostatistics and Medical Informatics, University of Wisconsin - Madison, Madison, WI, USA; Department of Computer Science, University of Wisconsin - Madison, Madison, WI, USA.

In addition to the development of beta amyloid plaques and neurofibrillary tangles, Alzheimer's disease (AD) involves the loss of connecting structures including degeneration of myelinated axons and synaptic connections. However, the extent to which white matter tracts change longitudinally, particularly in the asymptomatic, preclinical stage of AD, remains poorly characterized. In this study we used a novel graph wavelet algorithm to determine the extent to which microstructural brain changes evolve in concert with the development of AD neuropathology as observed using CSF biomarkers. Read More

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October 2018

[Clinical Analysis of Maintenance Therapy with Thalidomine and Bortezomib for Multiple Myeloma].

Zhongguo Shi Yan Xue Ye Xue Za Zhi 2018 Dec;26(6):1668-1674

Tianjin Medical University Cancer Institute and Hospital, National Clinical Research Center for Cancer, Tianjing Municipal Key Laboratory of Cancer Prevention and Therapy, Tianjin Municipal Clinical Research Center for Cancer,Tianjin 300060, China.E-mail:

Objective: To evaluate the therapeutic effect and adverse reactions of the maintenance therapies with Thalidomine or Bortezomib in the patients with newly diagnosed multiple myeloma (MM), so as to provide a reference for clinical treatment.

Methods: A retrospective analysis was conducted to compare the progression-free survival (PFS), overall survival (OS) and adverse reaction rate of 23 MM patients received the maintenance therapies of Bortezomib and of 68 MM patients received maintenance therapy of Thalidomine.

Results: The maintenance therapy with Bortezomib could extend the PFS of MM patients as compared with Thalidomine (PFS rate of patients on the maintenance therapy of Bortezomib in 12th, and 24th month was 100%, 88. Read More

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December 2018

The Persisting Significance of Oligoclonal Bands in the Dawning Era of Kappa Free Light Chains for the Diagnosis of Multiple Sclerosis.

Int J Mol Sci 2018 Nov 29;19(12). Epub 2018 Nov 29.

Clinical Neuroimmunology and Neurochemistry, Department of Neurology, Carl-Neuberg-Str. 1, 30625 Hannover, Germany.

The latest revision of the McDonald criteria of 2017 considers the evidence of an intrathecal immunoglobulin (IgG) synthesis as a diagnostic criterion for dissemination in time in multiple sclerosis. While the detection of oligoclonal bands is considered as the gold standard, determination of kappa free light chains might be a promising tool as a less technically demanding and cost saving method. However, data on the direct comparison between kappa free light chains and oligoclonal bands are limited and no study to date has used the highly sensitive method of polyacrylamide gels with consecutive silver staining for the demonstration of oligoclonal bands. Read More

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November 2018

Generation by phage display and characterization of drug-target complex-specific antibodies for pharmacokinetic analysis of biotherapeutics.

MAbs 2018 Dec 5:1-13. Epub 2018 Dec 5.

a Bio-Rad, Antibody Division , Puchheim , Germany.

Anti-idiotypic antibodies play an important role in pre-clinical and clinical development of therapeutic antibodies, where they are used for pharmacokinetic studies and for the development of immunogenicity assays. By using an antibody phage display library in combination with guided in vitro selection against various marketed drugs, we generated antibodies that recognize the drug only when bound to its target. We have named such specificities Type 3, to distinguish them from the anti-idiotypic antibodies that specifically detect free antibody drug or total drug. Read More

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December 2018

Pharmacodynamic Models of Differential Bortezomib Signaling Across Several Cell Lines of Multiple Myeloma.

CPT Pharmacometrics Syst Pharmacol 2018 Dec 4. Epub 2018 Dec 4.

Department of Pharmaceutical Sciences, University at Buffalo, SUNY, Buffalo, New York, USA.

The heterogeneous polyclonal nature of multiple myeloma complicates the identification of protein biomarkers predictive of drug response. In this study, a pharmacodynamic systems modeling approach was used to link in vitro bortezomib exposure and myeloma cell death. The exposure-response was integrated through a network of important protein biomarker dynamics activated by bortezomib in four myeloma cell lines. Read More

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December 2018
1 Read

Lymphoid Stress Surveillance Response Contributes to Vitiligo Pathogenesis.

Front Immunol 2018 20;9:2707. Epub 2018 Nov 20.

Department of Dermatology, University of Tartu, Tartu, Estonia.

Vitiligo is a chronic multifactorial depigmentation disorder characterized by the destruction and functional loss of melanocytes. Although a direct cytotoxic T cell attack is thought to be responsible for melanocyte damage, the events leading to the loss of self-tolerance toward melanocytic antigens are not understood. This research aimed to identify novel cellular and molecular factors that participate in vitiligo pathogenesis through the application of gene expression and immunofluorescence analysis of skin biopsy samples along with immunophenotyping of circulating cells. Read More

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November 2018
1 Read

Inhibition of autophagy by chloroquine makes chemotherapy in nasopharyngeal carcinoma more efficient.

Auris Nasus Larynx 2018 Dec 1. Epub 2018 Dec 1.

Division of Otolaryngology - Head and Neck Surgery, Graduate School of Medicine, Kanazawa University, Kanazawa, Japan. Electronic address:

Objectives: A combination of platinum-based chemotherapy and radiotherapy is the standard treatment for nasopharyngeal carcinoma (NPC). However, the efficacy of chemotherapy has reached a plateau. Many autophagy studies suggest that autophagy can either promote or suppress to cancer progression. Read More

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December 2018

Glycolysis inhibition via mTOR suppression is a key step in cardamonin-induced autophagy in SKOV3 cells.

BMC Complement Altern Med 2018 Dec 4;18(1):317. Epub 2018 Dec 4.

Department of Pharmacy, Fujian Provincial Maternity and Children's Hospital, Affiliated Hospital of Fujian Medical University, 18 Daoshan Road, Fuzhou, 350001, Fujian, China.

Background: Autophagy occurs in cells that undergoing nutrient deprivation. Glycolysis rapidly supplies energy for the proliferation of cancer cells. Cardamonin inhibits proliferation and enhances autophagy by mTORC1 suppression in ovarian cancer cells. Read More

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December 2018

Real-Time Monitoring of a Botulinum Neurotoxin Using All-Carbon Nanotube-Based Field-Effect Transistor Devices.

Sensors (Basel) 2018 Dec 3;18(12). Epub 2018 Dec 3.

Department of Chemistry, KAIST, 291 Daehak-ro, Yuseong-gu, Daejeon 34141, Korea.

The possibility of exposure to botulinum neurotoxin (BoNT), a powerful and potential bioterrorism agent, is considered to be ever increasing. The current gold-standard assay, live-mouse lethality, exhibits high sensitivity but has limitations including long assay times, whereas other assays evince rapidity but lack factors such as real-time monitoring or portability. In this study, we aimed to devise a novel detection system that could detect BoNT at below-nanomolar concentrations in the form of a stretchable biosensor. Read More

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December 2018

The ER Stress Inducer l-Azetidine-2-Carboxylic Acid Elevates the Levels of Phospho-eIF2α and of LC3-II in a Ca-Dependent Manner.

Cells 2018 Nov 30;7(12). Epub 2018 Nov 30.

Laboratory for Molecular and Cellular Signaling, Department of Cellular and Molecular Medicine & Leuven Kanker Instituut, KU Leuven, Campus Gasthuisberg O/N-1 B-802, Herestraat 49, BE-3000 Leuven, Belgium.

Accumulation of misfolded proteins in the endoplasmic reticulum (ER) activates the unfolded protein response (UPR) to reduce protein load and restore homeostasis, including via induction of autophagy. We used the proline analogue l-azetidine-2-carboxylic acid (AZC) to induce ER stress, and assessed its effect on autophagy and Ca homeostasis. Treatment with 5 mM AZC did not induce poly adenosine diphosphate ribose polymerase (PARP) cleavage while levels of binding immunoglobulin protein (BiP) and phosphorylated eukaryotic translation initiation factor 2α (eIF2α) increased and those of activating transcription factor 6 (ATF6) decreased, indicating activation of the protein kinase RNA-like ER kinase (PERK) and the ATF6 arms of the UPR but not of apoptosis. Read More

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November 2018

A brief introduction of IgG-like bispecific antibody purification: Methods for removing product-related impurities.

Authors:
Yifeng Li

Protein Expr Purif 2018 Dec 1;155:112-119. Epub 2018 Dec 1.

Technology and Process Development (TPD), WuXi Biologics, 288 Fute Zhong Road, Waigaoqiao Free Trade Zone, Shanghai, 200131, China. Electronic address:

Bispecific antibodies (bsAbs) are antibodies that can simultaneously bind two distinct targets or epitopes. Their dual-targeting capacity offers expanded therapeutic potential. Currently there is a strong interest in design and production of bsAbs to achieve improved efficacy through novel mechanisms of action. Read More

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December 2018
7 Reads

MicroRNA-29 enhances autophagy and cleanses exogenous mutant αB-crystallin in retinal pigment epithelial cells.

Exp Cell Res 2018 Dec 1. Epub 2018 Dec 1.

School of Optometry and Ophthalmology and the Eye Hospital, Wenzhou Medical University, PR China; State Key Laboratory of Optometry, Ophthalmology, and Visual Science, Wenzhou, Zhejiang, 325027, PR China. Electronic address:

Retinal pigment epithelial cells (RPEs), a pigmented cell layer in the outer retina, are constantly exposed to photo-oxidative stress. Autophagy relieves the stress by removing oxidative protein adducts, protein aggregates, and damaged mitochondria. We previously found that miR-29 is downregulated in choroid/RPE tissue in a model of exudative age-related macular degeneration (AMD), suggesting that miR-29 deficiency may contribute to autophagy inhibition and AMD progression. Read More

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December 2018
1 Read

Haematological response and overall survival in two consecutive Dutch patient cohorts with AL amyloidosis diagnosed between 2008 and 2016.

Amyloid 2018 Dec 4:1-7. Epub 2018 Dec 4.

a Department of Haematology , Utrecht University, University Medical Centre Utrecht , Utrecht , The Netherlands.

Background: Although survival has improved in recent decades, the short-term prognosis of patients with immunoglobulin light chain (AL) amyloidosis remains grim. We aimed to assess overall survival (OS) of AL amyloidosis patients by comparing cohorts in two consecutive time periods.

Methods: Data were collected and compared on 126 patients from two tertiary referral centres in The Netherlands during the time periods 2008-2012 and 2013-2016. Read More

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December 2018
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Bortezomib-based chemotherapy can improve renal and tubular functions in patients with light chain-associated Fanconi syndrome.

Ann Hematol 2018 Dec 4. Epub 2018 Dec 4.

Department of Hematology, Peking Union Medical College Hospital, Peking Union Medical College and Chinese Academy of Medical Sciences, Beijing, People's Republic of China.

Light chain-associated Fanconi syndrome (LCFS) is a disorder of renal proximal tubule due to immunoglobulin light chains. Cases of LCFS are rare and mostly sporadically reported, and treatment of this entity is still controversial. This single-center retrospective study included 22 patients diagnosed with LCFS in Peking Union Medical College Hospital. Read More

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December 2018

Three-Dimensional Organoids Reveal Therapy Resistance of Esophageal and Oropharyngeal Squamous Cell Carcinoma Cells.

Cell Mol Gastroenterol Hepatol 2019 14;7(1):73-91. Epub 2018 Sep 14.

Department of Digestive Surgery, Breast and Thyroid Surgery, Kagoshima University Graduate School of Medical and Dental Sciences, Kagoshima, Japan. Electronic address:

Background & Aims: Oropharyngeal and esophageal squamous cell carcinomas, especially the latter, are a lethal disease, featuring intratumoral cancer cell heterogeneity and therapy resistance. To facilitate cancer therapy in personalized medicine, three-dimensional (3D) organoids may be useful for functional characterization of cancer cells . We investigated the feasibility and the utility of patient-derived 3D organoids of esophageal and oropharyngeal squamous cell carcinomas. Read More

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September 2018

The roles of prostaglandin F in regulating the expression of matrix metalloproteinase-12 via an insulin growth factor-2-dependent mechanism in sheared chondrocytes.

Signal Transduct Target Ther 2018 23;3:27. Epub 2018 Nov 23.

College of Life and Health Sciences, Northeastern University, Shenyang, 110819 P. R. China.

Osteoarthritis (OA) was recently identified as being regulated by the induction of cyclooxygenase-2 (COX-2) in response to high fluid shear stress. Although the metabolic products of COX-2, including prostaglandin (PG)E, 15-deoxy-Δ-PGJ (15d-PGJ), and PGF, have been reported to be effective in regulating the occurrence and development of OA by activating matrix metalloproteinases (MMPs), the roles of PGF in OA are largely overlooked. Thus, we showed that high fluid shear stress induced the mRNA expression of MMP-12 via cyclic (c)AMP- and PGF-dependent signaling pathways. Read More

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November 2018
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Dynein Light Chain DLC-1 Facilitates the Function of the Germline Cell Fate Regulator GLD-1 in .

Genetics 2018 Dec 3. Epub 2018 Dec 3.

University of Montana

Developmental transitions of germ cells are often regulated at the level of post-transcriptional control of gene expression. In the germline, stem and progenitor cells exit the proliferative phase and enter meiotic differentiation to form gametes essential for fertility. The RNA-binding protein GLD-1 is a cell fate regulator that promotes meiosis and germ cell differentiation during development by binding to and repressing translation of target mRNAs. Read More

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December 2018

Diagnosis of cardiac amyloidosis: a systematic review on the role of imaging and biomarkers.

BMC Cardiovasc Disord 2018 Dec 4;18(1):221. Epub 2018 Dec 4.

3rd Cardiology Department, Ippokrateion General Hospital of Thessaloniki, Konstantinoupoleos 49, 55 642, Thessaloniki, GR, Greece.

Background: Cardiac Amyloidosis (CA) pertains to the cardiac involvement of a group of diseases, in which misfolded proteins deposit in tissues and cause progressive organ damage. The vast majority of CA cases are caused by light chain amyloidosis (AL) and transthyretin amyloidosis (ATTR). The increased awareness of these diseases has led to an increment of newly diagnosed cases each year. Read More

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December 2018
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Brain-originated peptides as possible biochemical markers of traumatic brain injury in cerebrospinal fluid post-mortem examination.

Folia Neuropathol 2018 ;56(2):97-103

The release of brain-originated peptides such as tau protein (MAPT), S-100β, neurofilament light chain (NFL), and glial fibrillary acidic protein (GFAP) into the cerebrospinal fluid (CSF) has been positively correlated with head injuries in clinical and basic research. In this study, we wanted to examine if selected CSF biomarkers (GFAP, NFL, and myelin basic protein - MBP) of head injury may be useful in post-mortem examination and diagnosis of forensic cases. The study was carried out using cases of head injury and cases of sudden death (cardiopulmonary failure, no injuries of the head as control group) provided by forensic pathologists at the Department of Forensic Medicine, Medical University of Warsaw. Read More

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January 2018