14,367 results match your criteria Leydig Cell Tumors


The methanolic extract of Guibourtia tessmannii (caesalpiniaceae) and selenium modulate cytosolic calcium accumulation, apoptosis and oxidative stress in R2C tumour Leydig cells: Involvement of TRPV1 channels.

Andrologia 2018 Dec 10:e13216. Epub 2018 Dec 10.

Department of Physiology, School of Medical Sciences, Health Campus, Universiti Sains Malaysia, Kelantan, Malaysia.

This study evaluated the effects of the methanolic extract of Guibourtia tessmannii (GT) and selenium (Se) on cell viability, intracellular calcium concentration ([Ca ] ), apoptosis and oxidative stress through transient receptor potential vanilloid 1 (TRPV1) channel activity in CCL-97 (R2C) tumour Leydig cells. The cells were divided into nine groups and treated as follows: (a)-Control, (b)-Capsazepine (CPZ, 0.1 mM, a TRPV1 channel blocker), (c)-Capsaicin (CAP, 0. Read More

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December 2018

Steroidogenic effects of extract on the levels of steroidogenic enzymes in mouse Leydig cells.

Anim Cells Syst (Seoul) 2018 8;22(6):407-414. Epub 2018 Nov 8.

Department of Urology, Chung-Ang University College of Medicine, Seoul, Republic of Korea.

In this study, we investigated the steroidogenic effect of extract on mouse TM3 Leydig cells, which produce male hormones by increasing the levels of steroidogenic enzymes. Steroidogenic enzymes are involved in the production of testosterone in the testis. To date, the steroidogenic effect of has not been reported. Read More

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November 2018

Effect of antiepileptic drug (Topiramate) and cold pressed ginger oil on testicular genes expression, sexual hormones and histopathological alterations in mice.

Biomed Pharmacother 2018 Dec 5;110:409-419. Epub 2018 Dec 5.

Agricultural Biochemistry Department, Faculty of Agriculture, Zagazig University, Zagazig, 44519, Egypt. Electronic address:

Sexual dysfunction in the epileptic patient is difficult to confirm whether it is ailment or therapy related. Antiepileptic drugs often use in reproductive age, through reproductive progress and maturation. On the other side, cold-pressed oils are rich in bioactive phytochemicals with health-promoting traits. Read More

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December 2018
2.023 Impact Factor

Ontogenesis of human fetal testicular steroidogenesis at early gestational age.

Steroids 2018 Dec 4;141:96-103. Epub 2018 Dec 4.

Department of Women's and Children's Health, Pediatric Endocrinology Unit, Karolinska Institute & University Hospital, Stockholm, Sweden. Electronic address:

The onset of steroidogenesis in human fetal testes (HFT) during the first trimester is poorly investigated. One important unresolved question is the ontogeny of steroidogenic enzymes and formation of steroidogenic pathways in the HFT at early pregnancy. Our aim was to explore steroidogenesis, the expression of steroidogenic enzymes and their maturation in the HFT at gestational weeks (GW) 8-12. Read More

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December 2018

Ethylene dimethane sulfonate (EDS) ablation of Leydig cells in adult rat depletes testosterone resulting in epididymal sperm granuloma: Testosterone replacement prevents granuloma formation.

Reprod Biol 2018 Dec 5. Epub 2018 Dec 5.

Department of Biology, Texas Woman's University, Denton, Texas, USA. Electronic address:

Sperm granuloma may develop in the epididymis following vasectomy or chemical insults. Inflammation due to sperm granuloma causes abdominal and scrotal pain. Prolonged and persistent inflammation in the epididymis due to sperm granuloma may lead to infertility. Read More

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December 2018

Vitamin C ameliorates the adverse effects of dexamethasone on sperm motility, testosterone level, and spermatogenesis indexes in mice.

Hum Exp Toxicol 2018 Dec 9:960327118816137. Epub 2018 Dec 9.

Department of Biology, Faculty of Science, Arak University, Arak, Iran.

Background:: Dexamethasone (DEX) is a common medicine that is capable of causing malformation in the male reproductive system. The aim of this study was to investigate the effect of vitamin C (Vit-C) on spermatogenesis indexes and daily sperm production (DSP) in adult mice treated with DEX.

Methods:: Male Naval Medical Research Institute (NMRI) mice were divided into four groups: Control, DEX (7 mg/kg/day), Vit-C (100 mg/kg/day), and DEX +Vit-C and treated for 7 days with intraperitoneal injection. Read More

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December 2018

Fetal Leydig cells dedifferentiate and serve as adult Leydig stem cells.

Development 2018 Dec 5;145(23). Epub 2018 Dec 5.

Department of Developmental Genetics, Institute of Advanced Medicine, Wakayama Medical University (WMU), Kimiidera, Wakayama 641-8509, Japan.

Previous studies have established that fetal Leydig cells (FLCs) and adult Leydig cells (ALCs) show distinct functional characteristics. However, the lineage relationship between FLCs and ALCs has not been clarified yet. Here, we reveal that a subset of FLCs dedifferentiate at fetal stages to give rise to ALCs at the pubertal stage. Read More

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December 2018

Biphasic effects of perfluorooctanoic acid on steroidogenesis in mouse Leydig tumour cells.

Reprod Toxicol 2018 Nov 30;83:54-62. Epub 2018 Nov 30.

Key Laboratory of Urban Environment and Health, Institute of Urban Environment, Chinese Academy of Sciences, Xiamen 361021, China. Electronic address:

Perfluorooctanoic acid (PFOA) is a persistent organic pollutant, which may possess endocrine disrupting properties. Herein, we investigated the possible mechanism(s) of toxicity and steroidogenesis in mouse Leydig cells. MLTC-1 (mouse Leydig tumour cells) cells were exposed to 0, 50, 100 or 200 μM PFOA for 48 h to ascertain their effects on the nuclear (membrane) receptor responses, steroidogenesis pathway and related regulated gene expression and steroid hormone secretion profiles. Read More

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November 2018
3 Reads

Immunolocalization of RFamide-related peptide 3 in a desert rodent Gerbillus tarabuli during seminiferous epithelium cycle.

Tissue Cell 2018 Dec 20;55:1-12. Epub 2018 Sep 20.

USTHB, University of Sciences and Technology of Houari Boumediene, Biological Sciences Faculty, Aride Area Research Laboratory, Algiers, Algeria.

Gerbillus tarabuli is a nocturnal seasonal breeder desert rodent with a main breeding season in spring and summer, and sexual quiescence in winter. This species is an interesting model for studying testis function in rodents. Therefore, the present study was performed firstly to investigate the stages of seminiferous epithelium cycle of Gerbillus tarabuli with a histological, morphometric and statistical study. Read More

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December 2018
1 Read

Down-regulation of steroidogenesis-related genes and its accompanying fertility decline in streptozotocin-induced diabetic male rats: ameliorative effect of metformin.

Andrology 2018 Dec 4. Epub 2018 Dec 4.

Department of Physiology, School of Medical Sciences, Universiti Sains Malaysia, Kubang Kerian, Malaysia.

Background: Metformin has long been used for glycemic control in diabetic state. Recently, other benefits of metformin beyond blood glucose regulation have emerged.

Objectives: To investigate the effect of metformin on the expression of testicular steroidogenesis-related genes, spermatogenesis, and fertility of male diabetic rats. Read More

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December 2018
1 Read

Ovarian Sex Cord-Stromal Tumors: Reflections on a 40-Year Experience With a Fascinating Group of Tumors, Including Comments on the Seminal Observations of Robert E. Scully, MD.

Authors:
Robert H Young

Arch Pathol Lab Med 2018 Dec;142(12):1459-1484

From the James Homer Wright Pathology Laboratories, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts.

Context.—: This year being the 60th anniversary of the publication of the excellent book Endocrine Pathology of the Ovary by John McLean Morris, MD, and Robert E. Scully, MD, the writer reflects on that work and in particular the remarkable contributions of its second author to our knowledge in this area. Read More

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December 2018
4 Reads

Subnutrition Effects During Pregnancy and Lactation on Mitosis, Apoptosis, and Androgen Receptor Expression in the Rat Testis.

Reprod Domest Anim 2018 Nov 30. Epub 2018 Nov 30.

Area of Histology and Embryology, Department of Morphology and Development, Veterinary Faculty, University of Uruguay.

In order to study the effect of undernutrition during gestation on the testicular development in rats and its impact on mitosis, apoptosis and the relative abundance of androgen receptor expression, twenty primiparous 3 months old Sprague-Dawley (Rattus norvegicus) rats, weighing 246 ± 4,0 grams when the experiment began, were mated by the same male. Control group (CG), n=10, fed ad libitum with water and rat chow and restricted group (RG, n=10), fed throughout pregnancy and until birth with 40% of the ad libitum maternal daily feed intake. Litters from both groups suckled for 25 days, RG with 14 pups/litter and CG with 8 pups/litter. Read More

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November 2018
2 Reads

Sexual Enhancing Effect of in Stress-Exposed Rats by Improving Dopaminergic and Testicular Functions.

Biomed Res Int 2018 25;2018:6452965. Epub 2018 Oct 25.

Department of Physiology, Faculty of Medicine, Khon Kaen University, 40002, Thailand.

In this study, we aimed to assess the effect and possible underlying mechanism of leaves extract on male sexual behaviors in stress-exposed rats. Male Wistar rats were orally given extract at doses of 25, 100, and 200 mg/kg BW before 12-hour-immobilization exposure for 14 days. Sexual behaviors, serum testosterone and corticosterone levels, TH-positive cells density in nucleus accumbens (NAc) and ventral tegmental area (VTA), MAO-B activity in NAc and medial preoptic area (MPOA), testis histology together with phosphodiesterase type-5 ( PDE-5) activity, and endothelial nitric oxide synthase (eNOS) expression in penis were evaluated after treatment. Read More

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October 2018
2 Reads

Perfluorododecanoic acid blocks rat Leydig cell development during prepuberty.

Chem Res Toxicol 2018 Nov 29. Epub 2018 Nov 29.

Perfluorododecanoic acid (PFDoA) has been used as a surfactant and may have reproductive toxicity. However, whether PFDoA influences Leydig cell development during prepuberty remains unknown. In the present study, 21-day-old male Sprague Dawley rats were gavaged 0, 5 or 10 mg/kg PFDoA from postnatal day 21 to 35. Read More

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November 2018
3 Reads

Ectopic Organogenesis after Allotransplantation of Freshly Removed or Cryopreserved Neonatal Testicle under the Renal Capsule in Rats.

Bull Exp Biol Med 2018 Dec 28;166(2):268-273. Epub 2018 Nov 28.

All-Russian Institute of Scientific and Technical Information, Russian Academy of Sciences, Moscow, Russia.

In experiments on white outbred male rats, a freshly removed (20 experiments) or cryopreserved (10 experiments) testicle from newborn rats (1-2 days after birth) was transplanted under the renal capsule after bilateral orchiectomy. In all experiments with transplantation of freshly removed testicle, it was engrafted. In 3 months, histological examination revealed the formation of mature seminiferous tubules, but spermatogenesis was blocked at the stage of spermatogonia; groups of proliferating Leydig cells in the loose connective tissue between the tubules were also seen. Read More

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December 2018
2 Reads

Utilisation of gonadotrophin-releasing hormone (GnRH) analogue to differentiate ovarian from adrenal hyperandrogenism in postmenopausal women.

Endocrinol Diabetes Metab Case Rep 2018 Nov 22;2018. Epub 2018 Nov 22.

Endocrinology, South Tipperary General Hospital, Clonmel, Ireland.

Postmenopausal hyperandrogenism is a relatively rare diagnosis resulting from excess androgen production from the adrenals or ovaries. The exclusion of malignant causes is a priority. Laboratory tests and imaging are utilised to help differentiate the source of excess androgens. Read More

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November 2018
10 Reads

Protective effect of vitamin E and vitamin C alone and in combination on testicular damage induced by sodium metabisulphite in rats: A stereological study.

Andrologia 2018 Nov 26:e13193. Epub 2018 Nov 26.

Endocrinology and Metabolism Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.

The existing investigation was directed to consider the protective role of vitamin C and E alone and in combination on sodium metabisulphite-induced damage on testicular. Experimental animals were received sodium metabisulphite (520 mg/kg) alone and in combination with vitamin E (100 mg/kg), vitamin C (100 mg/kg) and vitamin E + C, while the control groups received 0.9% saline solution and olive oil (the solvent of the vitamin E). Read More

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November 2018
7 Reads

Effects of spermatogenic cycle on Stem Leydig cell proliferation and differentiation.

Mol Cell Endocrinol 2018 Nov 23. Epub 2018 Nov 23.

Department of Anesthesiology, Perioperative Medicine, The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University, Wenzhou, Zhejiang, 325027, China; Department of Gynaecology and Obstetrics, The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University, Wenzhou, Zhejiang, 325027, China; Zhejiang Province Key Lab of Anesthesiology, The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University, Wenzhou, Zhejiang, 325027, China; Department of Biochemistry and Molecular Biology, Johns Hopkins Bloomberg School of Public Health, Baltimore, MD, 21205, USA. Electronic address:

We reported previously that stem Leydig cells (SLC) on the surfaces of rat testicular seminiferous tubules are able to differentiate into Leydig cells. The proliferation and differentiation of SLCs seem likely to be regulated by niche cells, including nearby germ and Sertoli cells. Due to the cyclical nature of spermatogenesis, we hypothesized that the changes in the germ cell composition of the seminiferous tubules as spermatogenesis proceeds may affect tubule-associated SLC functions. Read More

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November 2018
9 Reads

All-trans retinoic acid disrupts development in ex vivo cultured fetal rat testes. II: Modulation of mono-(2-ethylhexyl) phthalate toxicity.

Toxicol Sci 2018 Nov 26. Epub 2018 Nov 26.

Department of Pathology and Laboratory Medicine, Brown University, Providence, Rhode Island, USA.

Humans are universally exposed to low levels of phthalate esters (phthalates), which are used to plasticize polyvinyl chloride. Phthalates exert adverse effects on the development of seminiferous cords in the fetal testis through unknown toxicity pathways. To investigate the hypothesis that phthalates alter seminiferous cord development by disrupting retinoic acid signaling in the fetal testis, gestational day 15 fetal rat testes were exposed for 1-3 days to 10-6 M all-trans retinoic acid (ATRA) alone or in combination with 10-6 to 10-4 M mono-(2-ethylhexyl) phthalate (MEHP) in ex vivo culture. Read More

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November 2018

Characterization, recellularization, and transplantation of rat decellularized testis scaffold with bone marrow-derived mesenchymal stem cells.

Stem Cell Res Ther 2018 Nov 21;9(1):324. Epub 2018 Nov 21.

Tissue Engineering Lab, Department of Anatomical Sciences, School of Medicine, Shiraz University of Medical Sciences, Zand St., Shiraz, Fars, 7134845794, Iran.

Background: Regenerative medicine potentially offers the opportunity for curing male infertility. Native extracellular matrix (ECM) creates a reconstruction platform to replace the organs. In this study, we aimed to evaluate the efficiency of the testis decellularized scaffold as a proper niche for stem cell differentiation toward testis-specific cell lineages. Read More

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November 2018
2 Reads

Expression analysis of multifunctional RNA-binding protein hnRNP K during development of mammalian testis.

Pol J Vet Sci 2018 Jun;21(2):343-351

College of Life Science, Xinyang Normal University, Xinyang 464000, China.

Heterogeneous nuclear ribonucleoprotein K (hnRNP K), is a multifunctional protein that participates in a variety of regulatory processes of signal transduction and gene expression. To further characterize the significance of hnRNP K in different male germ cells, we investigated the expression profiles of hnRNP K at different developmental stages in pig and rat testes, and conducted a comparative analysis of expression patterns between these two species. In porcine testis development, both the mRNA and protein level of hnRNP K were down-regulated from 3 months to 8 months. Read More

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A Contemporary Review of Common Adult Non-germ Cell Tumors of the Testis and Paratestis.

Surg Pathol Clin 2018 Dec 17;11(4):739-758. Epub 2018 Oct 17.

Department of Pathology, Stanford University School of Medicine, 300 Pasteur Drive, L235, Stanford, CA 94305, USA. Electronic address:

This article provides a comprehensive review of non-germ cell tumors of the testis and paratestis in adults, incorporating the latest 2016 World Health Organization updates. Clinical features, gross pathologic findings, key morphologic details, immunohistochemical profiles, and differential diagnoses are covered, with an emphasis on how to resolve commonly encountered, and sometimes difficult, differential diagnoses. Read More

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December 2018
1 Read

Novel Compound Heterozygous Variants in the Gene in a Genetically Male Patient with Female External Genitalia.

J Clin Res Pediatr Endocrinol 2018 Nov 16. Epub 2018 Nov 16.

Department of Pediatrics, First Affiliated Hospital of Xinjiang Medical University, Urumqi 830054, Xinjiang Uygur Autonomous Region, China.

Objectives: The gene encodes a G-protein coupled receptor that plays a pivotal role in sexual differentiation in males, ovarian development in females, and in fertility via its interaction with luteinizing hormone (LH) and chorionic gonadotropin (CG). Inactive variants of the gene cause Leydig cell hypoplasia (LCH), which is a rare disease and one of the causes of disorder of sexual differentiation (DSD) in males. The aim of this work is to study the clinical and molecular characteristics of a 2-year-9-month old patient with type 1 LCH. Read More

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November 2018
4 Reads

Diagnostic pitfalls in ovarian androgen-secreting (Leydig cell) tumours: case series.

J Obstet Gynaecol 2018 Nov 14:1-6. Epub 2018 Nov 14.

d Institute of Endocrinology , Prague , Czech Republic.

Leydig cell tumours of the ovary are rare and represent a diagnostic challenge not only due to their sporadic incidence but also due to the seemingly normal imaging. We present three cases of pre- and postmenopausal women who were presented with severe clinical signs of hyperandogenism where modern imaging modalities (including computed tomography (CT), magnetic resonance imaging (MRI) and positron-emission tomography combined with computed tomography (PET-CT)) failed to identify the tumour. Two patients underwent non-expert ultrasound, CT and MRI examination with uniform conclusion that ovaries are of normal appearance. Read More

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November 2018
3 Reads

Quantification of Leydig cells and stromal hyperplasia in the postmenopausal ovary of women with endometrial carcinoma.

Hum Pathol 2018 Nov 11. Epub 2018 Nov 11.

Department of Pathology, Radboud University Medical Center, Nijmegen, 6525, GA, The Netherlands.

Endometrioid endometrial carcinomas (EECs) are correlated with high serum levels of androgens and estrogen. We hypothesized that Leydig cells and ovarian stromal hyperplasia contribute to postmenopausal ovarian androgen production, and are observed more frequently in EEC patients. Ovaries of postmenopausal women with EEC (n=36) or non-endometrioid endometrial carcinoma (NEEC) (n=19) were examined for the presence of hilar Leydig cells, and compared with ovaries resected for benign conditions (n=22). Read More

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November 2018
7 Reads

Delayed Puberty by Ziram Is Associated with Down Regulation of Testicular Phosphorylated AKT1 and SIRT1/PGC-1α Signaling.

Chem Res Toxicol 2018 Nov 27. Epub 2018 Nov 27.

Department of Obstetrics and Gynecology , The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University , 109 Xueyuan West Road , Wenzhou , Zhejiang 325027 , China.

Ziram is a dimethyldithiocarbamate fungicide, which may influence the male reproductive system as a potential endocrine disruptor. We interrogated the disruption of ziram on rat progenitor Leydig cell development. Prepubertal male Sprague-Dawley rats were orally treated with 0, 2, 4, or 8 mg/kg ziram for 2 weeks. Read More

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November 2018
5 Reads
3.530 Impact Factor

Vitamin A Promotes Leydig Cell Differentiation via Alcohol Dehydrogenase 1.

Front Endocrinol (Lausanne) 2018 29;9:644. Epub 2018 Oct 29.

Guangdong Provincial Key Laboratory of Bioengineering Medicine, Department of Cell Biology, Jinan University, Guangzhou, China.

Vitamin A (retinol) is important for multiple functions in mammals. In testis, the role of vitamin A in the regulation of testicular functions is clearly involved in rodents. It is essential for sperm production. Read More

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October 2018
9 Reads

Malignant ascites occurs most often in patients with high-grade serous papillary ovarian cancer at initial diagnosis: a retrospective analysis of 191 women treated at Bayreuth Hospital, 2006-2015.

Arch Gynecol Obstet 2018 Nov 10. Epub 2018 Nov 10.

Institute of Pathology, Klinikum Bayreuth, Preuschwitzer Strasse 101, 95445, Bayreuth, Germany.

Background: Malignant ascites often develops in patients with ovarian cancer, but there is a lack of more detailed characterization of the different histological subtypes.

Methods: Ascites specimens from patients with ovarian cancer who were treated at Bayreuth Hospital from 2006 to 2015, with follow-up until December 2016, were reevaluated retrospectively.

Results: A total of 191 women (mean age 64 years, range 48-79) were included, of whom 180 (94. Read More

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November 2018
9 Reads

Triclosan Suppresses Testicular Steroidogenesis via the miR-6321/JNK/ Nur77 Cascade.

Cell Physiol Biochem 2018 9;50(6):2029-2045. Epub 2018 Nov 9.

Key Lab of Birth Defects and Reproductive Health of National Health and Family Planning Commission, Chongqing Population and Family Planning Science and Technology Research Institute, Chongqing,

Background/aims: Triclosan (TCS), a broad-spectrum antibacterial and antifungal compound and an endocrine disruptor, has anti-androgenic properties and could adversely affect male reproduction and fertility.

Methods: To elucidate the underlying roles of miRNAs and the MAPK pathway in TCS-mediated repression of testicular steroidogenesis, Sprague-Dawley male rats were dosed daily with TCS for 31 days, and TM3 cells were exposed to TCS for 24 h after the pretreatments with the activator of JNK, Nur77 siRNA, or recombinant lentivirus vector for Nur77. Tissues and/or cells were analyzed by several techniques including transmission electron microscopy, lentivirus production, overexpression, gene silencing, luciferase reporter assay, chromatin immunoprecipitation, western blot, and real-time PCR. Read More

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December 2018
5 Reads

The efficacy of chronic zinc oxide nanoparticles using on testicular damage in the streptozotocin-induced diabetic rat model.

Acta Histochem 2018 Nov 6. Epub 2018 Nov 6.

Anatomy & Embryology Department, Faculty of Veterinary Medicine, Zagazig University, 44519, Zagazig, Egypt.

Testicular impairment is a common complication of Diabetes mellitus (DM). Zinc Oxide Nanoparticles (ZnO NPs) are a novel agent for Zn delivery with antidiabetic and antioxidant activities. However, few reports were recorded on it. Read More

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November 2018
4 Reads

Radiation effects on male fertility.

Andrology 2018 Nov 9. Epub 2018 Nov 9.

Department of Radiotherapy, Policlinico Umberto I "Sapienza" University of Rome, Rome, Italy.

Background: Spermatogenesis is a process of dynamic cell differentiation. Ionizing radiation impairs spermatogenesis, and spermatogonia are more radiosensitive than spermatocytes or spermatids. Consistent with this assumption and due to improvement in tumor curability, nowadays, fertility preservation represents a public health need. Read More

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November 2018

HIF 1 inhibits StAR transcription and testosterone synthesis in murine Leydig cells.

J Mol Endocrinol 2018 Oct 1. Epub 2018 Oct 1.

L Zhu, Biochemisty, Institute of Nautical Medicine, Nantong, China.

Hypoxia-inducible factor-1 (HIF1) is a critical transcription factor involved in cell response to hypoxia. Under physiological conditions, its a subunit is rapidly degraded in most tissues except testes. HIF1 is stably expressed in Leydig cells, which are the main source of testosterone for male, and might bind to the promoter region of steroidogenic acute regulatory protein (Star), which is necessary for the testosterone synthesis, according to software analysis. Read More

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October 2018
3 Reads

Virilising ovarian tumors: a single center experience.

Endocr Connect 2018 Nov 1. Epub 2018 Nov 1.

N Shah, Mumbai, 400012, India.

Aims: Literature on virilising ovarian tumors (VOT) is limited to case reports and series reporting single pathological type. We have analyzed the clinical, hormonal, radiological, histological, management and outcome data of VOT.

Materials And Methods: This retrospective study was conducted at a tertiary health care center from Western India. Read More

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November 2018
3 Reads

Fsh stimulates Leydig cell Wnt5a production, enriching zebrafish type A spermatogonia.

J Endocrinol 2018 Dec;239(3):351-363

Reproductive Biology Group, Division Developmental Biology, Department of Biology, Institute of Biodynamics and Biocomplexity, Faculty of Science, University of Utrecht, NL-3584 CH Utrecht, The Netherlands.

Follicle-stimulating hormone (Fsh) modulates vertebrate spermatogenesis by regulating somatic cell functions in the testis. We have found previously that zebrafish Fsh stimulated the differentiating proliferation of type A undifferentiated spermatogonia (Aund) in an androgen-independent manner by regulating the production of growth factors and other signaling molecules in both Sertoli (SCs) and Leydig cells (LCs). For example, Fsh triggered the release of Igf3 that subsequently activated β-catenin signaling to promote the differentiating proliferation of Aund. Read More

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December 2018
2 Reads

Molecular hydrogen may enhance the production of testosterone hormone in male infertility through hormone signal modulation and redox balance.

Med Hypotheses 2018 Dec 6;121:6-9. Epub 2018 Sep 6.

Department of Environmental Medical Biology, Wonju College of Medicine, Yonsei University, Wonju, Gangwon 26426, Republic of Korea; Institute for Poverty Alleviation and International Development, Yonsei University, Wonju, Gangwon 26493, Republic of Korea. Electronic address:

Since the discovery of molecular hydrogen (H) as a selective scavenger of free radicals like reactive oxygen species (ROS) and reactive nitrogen species (RNS), numerous studies have proved the potential application of H in therapeutic and preventative medicine. Moreover, H can regulate the intracellular signal as a signal modulator. However, it is still unclear in cell signaling involved in testosterone hormone production. Read More

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December 2018
8 Reads

Dicyclohexyl phthalate blocks Leydig cell regeneration in adult rat testis.

Toxicology 2018 Nov 1;411:60-70. Epub 2018 Nov 1.

Department of Anesthesiology, The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University, Wenzhou, Zhejiang 325027, China. Electronic address:

Dicyclohexyl phthalate (DCHP) is a phthalate plasticizer with a ring structure in the alcohol moiety. The objective to the current study was to determine the effects of DCHP on Leydig cell regeneration in the adult rat-testis. Adult male Sprague Dawley rats received intraperitoneally an injection of ethane dimethane sulfone (EDS) to eliminate all Leydig cells in the testis and then were divided into 4 groups of 0 (control), 10, 100, and 1000 mg/kg/day DCHP. Read More

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November 2018
3 Reads
3.620 Impact Factor

Testis sparing surgery for small testicular masses and frozen section assessment.

Cent European J Urol 2018 16;71(3):304-309. Epub 2018 Aug 16.

Department of Urology, Frimley Health NHS Foundation Trust, Wexham Park Hospital Slough, Berkshire, United Kingdom.

Introduction: We present our experience with patients who had suspected testicular masses, managed by a frozen section assessment and testicular sparing surgery.

Material And Methods: We performed a retrospective review of all patients over the last 5 years, who underwent a frozen section assessment and testicular sparing surgery for small testicular lesions. The frozen section assessment was compared with the final histology. Read More

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August 2018
2 Reads

Infectivity of adeno-associated virus serotypes in mouse testis.

BMC Biotechnol 2018 Nov 1;18(1):70. Epub 2018 Nov 1.

Institute for Stem Cell Biology and Regenerative Medicine, GKVK Campus, Bellary Road, Bengaluru, 560065, India.

Background: Recombinant adeno-associated viruses (AAVs) are emerging as favoured transgene delivery vectors for both research applications and gene therapy. In this context, a thorough investigation of the potential of various AAV serotypes to transduce specific cell types is valuable. Here, we rigorously tested the infectivity of a number of AAV serotypes in murine testis by direct testicular injection. Read More

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November 2018
1 Read

Regulation of anti-Müllerian hormone (AMH) in males and the associations of serum AMH with the disorders of male fertility.

Asian J Androl 2018 Oct 26. Epub 2018 Oct 26.

Center for Reproductive Medicine, Department of Obstetrics and Gynecology, Peking University Third Hospital, Beijing 100191, China.

Anti-Müllerian hormone (AMH) is a functional marker of fetal Sertoli cells. The germ cell number in adults depends on the number of Sertoli cells produced during perinatal development. Recently, AMH has received increasing attention in research of disorders related to male fertility. Read More

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October 2018
6 Reads

A novel testis-specific long noncoding RNA, Tesra, activates the Prss42/Tessp-2 gene during mouse spermatogenesis.

Biol Reprod 2018 Oct 30. Epub 2018 Oct 30.

Graduate School of Life Science, Hokkaido University, Sapporo 060-0810, Japan.

The progression of spermatogenesis is precisely controlled by meiotic stage-specific genes, but the molecular mechanism for activation of such genes is still elusive. Here we found a novel testis-specific long noncoding RNA (lncRNA), Tesra, that was specifically expressed in the mouse testis at the Prss/Tessp gene cluster on chromosome 9. Tesra was transcribed downstream of Prss44/Tessp-4, starting within the gene, as a 4,435-nucleotide transcript and developmentally activated at a stage similar to that for Prss/Tessp genes. Read More

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October 2018
10 Reads

Chlorpyrifos Induction of Testicular-Cell Apoptosis through Generation of Reactive Oxygen Species and Phosphorylation of AMPK.

J Agric Food Chem 2018 Nov 15;66(47):12455-12470. Epub 2018 Nov 15.

College of Animal Science and Technology , Northwest A&F University , Yangling , Shaanxi 712100 , China.

Chlorpyrifos (CPF) is the most frequently applied insecticide. Aside from effects on the neuronal cholinergic system, previous studies suggested a potential relationship between CPF exposure and male infertility; however, the molecular mechanism remains elusive. The aim of this study was to investigate the toxic effect of CPF on testicular cells and the potential mechanism via in vitro and in vivo experiments. Read More

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November 2018
2 Reads

Rare case of occult testosterone-producing ovarian tumor that was diagnosed by selective venous hormone sampling.

Reprod Med Biol 2018 Oct 21;17(4):504-508. Epub 2018 Jun 21.

Department of Obstetrics and Gynecology Sapporo Medical University School of Medicine Sapporo Japan.

Case: A 32 year old woman was referred because of secondary amenorrhea, hirsutism, and voice deepening.

Outcome: The blood testosterone level was markedly high. A transvaginal ultrasound revealed a small region in the left ovary, but whether or not it was a tumor was unclear. Read More

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October 2018
1 Read

Cyanidin-3- O-glucoside at Low Doses Protected against 3-Chloro-1,2-propanediol Induced Testis Injury and Improved Spermatogenesis in Male Rats.

J Agric Food Chem 2018 Oct 30. Epub 2018 Oct 30.

Department of Food Science and Engineering, Institute of Food Safety and Nutrition, Guangdong Engineering Technology Center of Food Safety Molecular Rapid Detection , Jinan University , Guangzhou 510632 , PR China.

In recent decades, the capability of mankind spermatogenesis is declining due to various threats. Anthocyanins as colorful polyphenols possess beneficial functions for the organisms, including Leydig cells, but their effects on male spermatogenesis remain underexplored. In our study, the protective effect of cyanidin-3- O-glucoside (C3G) was investigated on the 3-chloro-1,2-propanediol (3-MCPD) caused rat spermatogenic disorders. Read More

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October 2018
5 Reads

A perivascular niche for multipotent progenitors in the fetal testis.

Nat Commun 2018 10 30;9(1):4519. Epub 2018 Oct 30.

Division of Reproductive Sciences, Cincinnati Children's Hospital Medical Center, 3333 Burnet Avenue, MLC 7045, Cincinnati, OH, 45229, USA.

Androgens responsible for male sexual differentiation in utero are produced by Leydig cells in the fetal testicular interstitium. Leydig cells rarely proliferate and, hence, rely on constant differentiation of interstitial progenitors to increase their number during fetal development. The cellular origins of fetal Leydig progenitors and how they are maintained remain largely unknown. Read More

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October 2018
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Chemokine-like receptor 1 deficiency leads to lower bone mass in male mice.

Cell Mol Life Sci 2018 Oct 29. Epub 2018 Oct 29.

Laboratory for Reproductive Health, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen, 518055, China.

The adipokine Chemerin and its receptor, chemokine-like receptor 1 (CMKLR1), are associated with osteoblastogenic differentiation of mesenchymal stem cells (MSCs) and osteoclastogenic differentiation of osteoclast precursors in vitro, suggesting that CMKLR1 would affect the bone mineral density (BMD). However, the role of CMKLR1 on BMD in vivo remains unknown. Here, using CMKLR1 knockout mouse model, we unveiled that CMKLR1 effected the amount of Leydig cells in testis and regulated androgen-dependent bone maintenance in male mice, which exhibited lower serum testosterone levels, thereby reducing the trabecular bone mass. Read More

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October 2018
5 Reads
5.810 Impact Factor

C-Type Natriuretic Peptide/Natriuretic Peptide Receptor 2 Is Involved in Cell Proliferation and Testosterone Production in Mouse Leydig Cells.

World J Mens Health 2018 Oct 23. Epub 2018 Oct 23.

College of Basic Medical Science, Jiujiang, China.

Purpose: This study investigated the role of natriuretic peptide receptor 2 (NPR2) on cell proliferation and testosterone secretion in mouse Leydig cells.

Materials And Methods: Mouse testis of different postnatal stages was isolated to detect the expression C-type natriuretic peptide (CNP) and its receptor NPR2 by quantitative reverse transcription polymerase chain reaction (RT-qPCR). Leydig cells isolated from mouse testis were cultured and treated with shNPR2 lentiviruses or CNP. Read More

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October 2018
6 Reads

Haploid Germ Cells Generated in Organotypic Culture of Testicular Tissue From Prepubertal Boys.

Front Physiol 2018 9;9:1413. Epub 2018 Oct 9.

Department of Gynecology-Andrology, Cliniques Universitaires Saint-Luc, Brussels, Belgium.

While in mice various studies have described the completion of spermatogenesis using either organotypic culture of prepubertal testicular tissue or 3D culture of isolated cells, in humans it has not been possible to achieve germ cell differentiation from immature testicular tissue (ITT). In our study, we evaluated the ability of human ITT to differentiate via a long-term organotypic culture of frozen-thawed 1 mm testicular fragments from five prepubertal boys in two different culture media. Tissue and supernatants were analyzed at regular intervals up to day 139. Read More

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October 2018
4 Reads

Sertoli-Leydig Cell Tumour and Mutation: A Case Report and Review of the Literature.

Case Rep Obstet Gynecol 2018 25;2018:7927362. Epub 2018 Sep 25.

St George's Hospital and the Royal Marsden Hospital, UK.

Sertoli-Leydig cell tumours of the ovary (SLCT) are rare tumours predominantly caused by mutations in the gene. We present a patient with a unilateral SLCT who had an underlying germline gene mutation. We discuss the underlying pathology, risks, and screening opportunities available to those with a mutation in this gene as SLCT is only one of a multitude of other tumours encompassing syndrome. Read More

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September 2018

Testicular adrenal rest tumour in an adult patient with congenital adrenal hyperplasia: a case report and review of literature.

J Surg Case Rep 2018 Oct 16;2018(10):rjy255. Epub 2018 Oct 16.

Department of Urology, National Hospital of Sri Lanka, Colombo, Sri Lanka.

Late presentations of testicular adrenal rest tumours (TART) are rarely seen in males with congenital adrenal hyperplasia, and are often misdiagnosed as primary testicular germ cell tumours. We report a case of bilateral TART in an adult patient with congenital adrenal hyperplasia who had defaulted treatment. He presented with a gradually increasing swelling of the left testis and genital examination revealed a large hard indurated swelling of both epididymes more prominent on the left side. Read More

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October 2018
6 Reads